CN107434305A - A kind of water purification agent of rich defect carbon carrier fixation of microbe and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of microbial water purifiers, and in particular relates to a water purifier for immobilizing microorganisms on a defect-rich carbon carrier and a preparation method thereof. The defect-rich carbon carrier is placed in the culture medium, and the compound strain is inoculated into the culture medium at the same time, and the water purifying agent is obtained through shaking co-cultivation. Because the surface of the carbon carrier of the water purifier has oxygen-containing functional groups, it overcomes the hydrophobicity problem of the general carbon carrier, and the surface of the carbon carrier is rich in defects and secondary pore structures, providing more active sites for the adsorption reaction and improving The adsorption efficiency of pollutants is improved; the co-cultivation of defect-rich carbon carriers containing hydrophilic groups and microorganisms is beneficial to the adsorption and fixation of microorganisms by carbon carriers, and at the same time, the organic matter and pollutants adsorbed on the carrier carbon are efficiently utilized and synergistically degraded by microorganisms, respectively. , so the water purifier has the characteristics of being hydrophilic but easy to precipitate, and has the advantages of double purification of carbon adsorption and biodegradation, and is very suitable for large-scale treatment of aquaculture anaerobic sewage.

Description

A water purifying agent for immobilizing microorganisms on a defect-rich carbon carrier and its preparation method

technical field

The invention belongs to the technical field of microbial water purifiers, and in particular relates to a water purifier for immobilizing microorganisms on a defect-rich carbon carrier and a preparation method thereof.

technical background

In the large-scale development of animal husbandry, unified management has reduced costs and improved economic benefits, but serious environmental pollution caused by a large number of concentrated sewage discharges requires urgent treatment. At home and abroad, there are two main types of aquaculture sewage treatment methods: resource utilization and standard discharge, which can be classified into three types: returning to the field, natural treatment and industrial treatment, each of which has its own advantages and disadvantages. The purpose of my country's aquaculture sewage treatment is to control and reduce the harm caused by it, carry out comprehensive utilization, and finally achieve the goals of reduction, recycling, harmless and standard discharge, reduce the impact on crop soil quality and crop quality, and reduce the impact on crop soil quality. Potential pollution risks of water body, soil environment and climate environment.

In recent years, according to the characteristics of agricultural environmental pollutants, researchers have conducted systematic research on the treatment methods and processes of aquaculture sewage, using chemical methods and biological methods to adsorb nitrogen, phosphorus, heavy metal ions and organic molecules. , separation and biological/chemical degradation, a single treatment method has achieved the goal of reduction and resource utilization to a certain extent, but it is difficult to achieve complete harmlessness and national emission standards. In the research process, activated carbon is usually used as an adsorbent for sewage pollutants. It can improve the color and turbidity of sewage, and it also contains macropores, mesopores and micropores, so it is suitable for small molecules and heavy metals. Ions can also undergo adsorption, and can also be pressed into small columnar particles as the carrier of biological water purifiers for the purification of anaerobic biogas slurry in livestock and poultry breeding, and a single bacterial strain is fixed on the carbon carrier to form a bacterial carbon water purifier , its sewage purification effect is often not as ideal as the bacterial carbon purification effect formed by compound strains. The patent with the publication number CN 104261570 A discloses an anaerobic sewage purifier for livestock and poultry breeding, which is composed of a composite microbial strain and an activated carbon carrier. Solid cylindrical activated carbon made by shaping. The coal-based activated carbon used and commercially available carbon materials (activated carbon AC, carbon nanotube CNT, graphite GC, etc.) have many advantages, such as high porosity, large specific surface, good stability, non-toxic and non-polluting-environmentally friendly, etc. ; but its surface has fewer functional groups and does not contain hydrophilic groups (-COOH, -OH), so the dispersion of the purifying agent in water is poor, which hinders full contact with pollutants in sewage, and has hydrogen bonds and Ammonia-nitrogen substances with lone electron pairs are difficult to interact electrostatically and cannot fully exert the adsorption effect of carbon-based supports on pollutants. Therefore, it is very necessary to control the surface affinity/hydrophobicity of carbon materials. At present, the commonly used method is to use concentrated acid (concentrated HNO ₃ , concentrated H ₂ SO ₄ ) to activate the surface of inert carbon and oxidize the surface carbon to form oxygen-containing functional groups (-COOH, -OH, -C=O). Polluted nitrogen oxides and sulfur oxides cause secondary pollution; and use alkali at a high temperature of about 600-900°C to react with carbon in an inert gas environment to etch out secondary pore structures and surface active sites, These methods have harsh reaction conditions and require the reaction vessel to be resistant to acid, alkali and high temperature, and there are many deficiencies in the actual operation and application process.

Contents of the invention

The object of the present invention is to provide a water purifier for immobilizing microorganisms on a defect-rich carbon carrier and a preparation method thereof in view of the deficiencies in the prior art. The carbon carrier used is rich in hydrophilic groups, which makes the purifier have better compatibility with water, overcomes the hydrophobicity problem of general carbon carriers, and is more conducive to the adsorption and fixation of microbial strains. The contact is better, the adsorption efficiency of pollutants is improved, and the adsorption of pollutants is increased due to the rich secondary pore structure and surface active sites. The water purifier for immobilizing microorganisms on the defect-rich carbon carrier has double purification functions of carbon adsorption and biodegradation, and simultaneously realizes the recycling of organic matter by microorganisms and the synergistic and efficient degradation of pollutants.

In order to realize the above-mentioned purpose of the invention, the present invention adopts following technical scheme:

A water purifying agent for immobilizing microorganisms with defect-rich carbon carriers: the defect-rich carbon carrier is placed in Luria-Bertani medium, and the compound strain is inoculated into Luria-Bertani medium at the same time, co-cultivated by shaking and dried to obtain Water purifying agent; the preparation method of described defect-rich carbon carrier comprises the following steps:

(1) Put 90g of mixed alkali of NaOH and KOH in a 200 ml reactor liner, and melt it at 165°C for 2 hours to obtain a clarified molten alkali;

(2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 12 hours to generate defect-rich powder carbon precursor materials;

(3) After the carbon precursor material was washed with water and absolute ethanol in sequence, it was dried at 80°C for 12 hours to obtain a powder carbon precursor;

(4) The powdered carbon precursor was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous and defect-rich carbon powder material (total pore volume about 0.05-0.06 cm ³ /g);

(5) Disperse the porous defect-rich carbon powder material in ethanol, then drop polyethylene glycol into the dispersion liquid, stir well to form a jelly, and press it to make the carbon carrier of the microbial water purifier.

The formula of the Luria-Bertani medium is: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000 mL, pH 7.0.

The mass ratio of NaOH and KOH in the mixed alkali described in step (1) is 46.5:43.5.

A method for preparing a water purifying agent for immobilizing microorganisms with a rich-deficient carbon carrier as described above: 10g of a rich-defective carbon carrier is added to 50-150 ml Luria-Bertani medium, and Bacillus licheniformis , marsh red false Rhodop seudanonas palustris , Chlorella vulgaris , Streptococcus , Thiobacillus Thiooxidant were inoculated into Luria-Bertani medium at a mass ratio of 4:5:1:4:1, and the inoculation amount of the composite strain was 1 -2%, shake and co-culture at 4°C and 150 rpm for 48-72 hours, and finally remove the carbon carrier and dry it in a constant temperature box at 4°C to obtain the water purifier; the effective number of viable bacteria on the carbon carrier is 7×10 ⁸ ～6×10 ⁹ CFU/g, the bacterial carbon binding rate reached 97.8%.

During the co-cultivation process of defect-rich carbon carriers and microorganisms, hydrophilic Gram-negative bacteria (Thiobacillus oxidans/Rhodopseudomonas palustris) are electropositive because the cell surface components are mainly lipopolysaccharides, which can It is adsorbed and fixed on the carbon carrier with negatively charged groups through electrostatic interaction; while the cell wall of Gram-positive bacteria (Chlorella/Bacillus/Streptococcus) is composed of a thick and dense layer of 5 glycine cross-linked peptide polymers Composed of sugar and teichoic acid, the negative charge on the surface makes it hydrophobic. In the early stage of culture and growth, its hydrophobicity can be used to diffuse in the nutrient solution, and a variety of resources can be obtained to meet the needs of proliferation. During the growth process, the hydrophobicity of microorganisms decreases so that It can float freely in water without environmental stress, improve survival ability, and further increase the hydrophilicity of microorganisms in the late growth stage, which is convenient for adsorption and fixation on hydrophilic carbon carriers with negatively charged groups. Carbon supports with functional groups are beneficial to the adsorption and immobilization of microbial strains.

A water purifier for immobilizing microorganisms on a defect-rich carbon carrier as described above is stored at 4°C and placed at room temperature for 8-12 hours before use to restore biological activity and is used in the treatment of aquaculture anaerobic sewage.

The strains used in the above raw materials can be conveniently isolated from nature by those skilled in the art through conventional strain identification and isolation methods, or strains publicly purchased through commercial channels. The strains used in the present invention can be purchased from suppliers such as China Microbial Strains Network - Beijing Beina Chuanglian Biotechnology Research Institute.

Above-mentioned bacterial classification is cultivated according to existing routine method:

Ⅰ Medium for Bacillus licheniformis: tryptone 5g, yeast extract 15g, K ₂ HPO ₄ 3g, glucose 2g, distilled water 1000mL, pH 7.5;

Ⅱ Medium for Rhodopseudomonas palustris: 1.0g NH ₄ Cl, 0.5g K ₂ HPO ₄ , 3.0g NaHCO ₃ , 2.0g yeast extract, 1000 mL distilled water, pH 7.2;

Ⅲ Chlorella common culture solution formula: NH ₄ NO ₃ 50-100 mg, K ₂ HPO ₄ 5 mg, FeC ₆ H ₅ O ₇ 0.1-0.5 mg, seawater 1000 mL;

Streptococcus IV culture medium: beef powder 10.0g, tryptone 20.g, glucose 2.0g, NaHCO ₃ 2.0g, NaCl 2.0g, Na ₂ HPO ₄ 0.4g, distilled water 1000 mL, pH 7.8;

Ⅴ Culture medium for Thiobacillus oxidans: 5.0 g of tryptone, 1.0 g of KH ₂ PO ₄ , 0.5 g of MgSO ₄ ·7H ₂ O, 10 g of glucose, and 1000 mL of distilled water.

The beneficial effects of the present invention are:

1) Put the microbial water purifier of the present invention into the aquaculture anaerobic sewage, the water purifier can use microorganisms to degrade the macromolecular organic matter in the sewage, remove most of the pollutants in the sewage, and achieve the purpose of purifying the sewage;

2) Since the carbon carrier of the microbial water purifier uses organic compounds as raw materials, the surface of the prepared carbon carrier is rich in oxygen-containing functional groups (-OH, -COOH, etc.), and these charged groups interact with the hydrogen protons/hydroxide ions in the water Electrostatic interaction occurs, which makes the carbon carrier and water have better compatibility, overcomes the hydrophobicity problem of general carbon carriers, and has better contact with pollutants; at the same time, the charged groups on the surface of the carbon carrier will interact with nitrogen, The lone pair electrons of phosphorus and sulfur have electrostatic interaction, which improves the adsorption efficiency of pollutants; secondly, the calcination process at 300 °C also improves the purity and porosity of carbon powder, and the molten solid alkali environment makes carbon materials obtain surface defects and The secondary pore structure provides more active sites for the adsorption reaction and increases the adsorption of pollutants;

3) By co-cultivating the hydrophilic carbon carrier and microorganisms, the effective number of viable bacteria on the carbon carrier of the prepared water purifier is 7×10 ⁸ ~ 6×10 ⁹ CFU/gCFU/g, and the bacterial carbon binding rate reaches 97.8 %, which improves the adsorption and fixation efficiency of carbon carrier to microorganisms;

4) The water purifier for immobilizing microorganisms on the defect-rich carbon carrier, because the microorganisms colonized on the activated carbon can efficiently degrade the organic matter adsorbed on the carbon as nutrients, which not only purifies the water quality, but also uses the products of their own metabolism as mutual utilization Nutrient substrate, improve microbial activity, and promote the synergistic degradation of pollutants by microbial flora, so it has the advantages of double purification of carbon adsorption and biodegradation, hydrophilic but easy to precipitate, stable purification effect and easy handling, etc., very suitable for Large-scale treatment of aquaculture anaerobic wastewater.

detailed description

In order to further disclose rather than limit the present invention, the present invention will be further described in detail below in conjunction with examples.

A kind of preparation method of the water purifying agent of rich defect carbon carrier immobilized microorganism, concrete steps are:

1. Preparation of carbon supports with hydrophilic functional groups and defects on the surface

1) Put NaOH+KOH=90g (NaOH:KOH=46.5:43.5, mass ratio) in a 200 ml reactor liner and melt at 165°C for 2 hours to obtain clarified molten lye;

2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 12 hours to generate defect-rich powder carbon precursor material A;

3) Wash the above-mentioned carbon precursor A with water until the pH value of the washing solution is about 7, remove water-soluble impurities, and then centrifuge to obtain the lower precipitate; the lower precipitate is then washed with absolute ethanol to remove water-insoluble organic impurities, and finally centrifuged The precipitate was dried overnight at 80°C to obtain defect-rich powdery carbon precursor sample A;

4) The carbon precursor sample A was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous and defect-rich carbon powder B (total pore volume about 0.05-0.06 cm ³ /g);

5) Put 0.5-1.0 g of porous defect-rich carbon powder B in an agate mortar, add 2-4 ml of ethanol, stir for 30 minutes to disperse the carbon powder evenly in ethanol, and then add 1-2 ml of polyethylene Glycol (PEG400), fully stirred to make the powder carbon into a jelly, put it into the tablet tank of the tablet press (cylindrical, tablet type) or a pressure-resistant mold, and dry it in an environment of 80 ° C for 24 Hours, and finally press molding under 10-15MPa pressure to make the carbon carrier of the microbial water purifying agent.

2. Add 10 g of defect-rich carbon carriers to 50-150 ml Luria-Bertani medium, and add Bacillus licheniformis , Rhodopseudomonas palustris , Chlorella vulgaris , Streptococcus , oxidized Thiobacillus Thiooxidant was inoculated into Luria-Bertani medium at a mass ratio of 4:5:1:4:1, the inoculum amount of the compound strain was 1-2%, and co-cultivated by shaking at 4°C and 150rpm for 48-72h. Finally, remove the carbon carrier and dry it in a constant temperature box at 4°C to obtain a water purifier; the effective number of viable bacteria on the carbon carrier is 7×10 ⁸ to 6×10 ⁹ CFU/g, and the bacterial carbon binding rate reaches 97.8 %; The water purifier stored at 4°C can be placed in the sewage directly for 8-12 hours before use to restore its biological activity.

Example 1

A kind of preparation method of the water purifying agent of rich defect carbon carrier immobilized microorganism, concrete steps are:

(1) Preparation of carbon supports with hydrophilic functional groups and defects on the surface

1) Put NaOH+KOH=90g (NaOH:KOH=46.5:43.5, mass ratio) in a 200 ml reactor liner and melt at 165°C for 2 hours to obtain clarified molten lye;

2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 12 hours to generate defect-rich powder carbon precursor material A;

3) Wash the above-mentioned carbon precursor A with water until the pH value of the washing solution is about 7, remove water-soluble impurities, and then centrifuge to obtain the lower precipitate; the lower precipitate is then washed with absolute ethanol to remove water-insoluble organic impurities, and finally centrifuged The precipitate was dried overnight at 80°C to obtain defect-rich powdery carbon precursor sample A;

4) The carbon precursor A was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous and defect-rich carbon powder material B;

5) Put 0.8 g of porous defective carbon powder B in an agate mortar, add 3 ml of ethanol, stir for 30 minutes to disperse the carbon powder evenly in the ethanol, then add 1.6 ml of polyethylene glycol (PEG400), fully Stir to make the powder carbon into a jelly, put it into the tablet tank of the tablet press (cylindrical, tablet type) or a pressure-resistant mold, dry it in an environment of 80°C for 24 hours, and finally press it under a pressure of 15 MPa. Press down to form the carbon carrier of the microbial water purifier.

(2) Add 10 g of defect-rich carbon carriers to 150 ml of Luria-Bertani medium, and add Bacillus licheniformis , Rhodopseudomonas palustris , Chlorella vulgaris , Streptococcus , sulfur oxide The bacillus Thiobacillus Thiooxidant was inoculated into Luria-Bertani medium at a mass ratio of 4:5:1:4:1, the inoculum amount of the compound strain was 1%, and the co-cultivation was shaken at 4°C and 150 rpm for 72 hours, and finally the carbon was removed. The carrier is dried in a constant temperature box at 4°C to obtain a water purifier; the effective number of viable bacteria on the carbon carrier is 6×10 ⁹ CFU/g, and the bacterial carbon binding rate reaches 97.8%; the net The water agent can be placed in the sewage directly for 10 hours at room temperature to recover its biological activity before use.

Example 2

A kind of preparation method of the water purifying agent of rich defect carbon carrier immobilized microorganism, concrete steps are:

(1) Preparation of carbon supports with hydrophilic functional groups and defects on the surface

1) Put NaOH+KOH=90g (NaOH:KOH=46.5:43.5, mass ratio) in a 200 ml reactor liner and melt at 180°C for 2 hours to obtain clarified molten lye;

2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 10 hours to generate defect-rich powder carbon precursor material A;

3) Wash the above-mentioned carbon precursor A with water until the pH value of the washing solution is about 7, remove water-soluble impurities, and then centrifuge to obtain the lower precipitate; the lower precipitate is then washed with absolute ethanol to remove water-insoluble organic impurities, and finally centrifuged The precipitate was dried overnight at 80°C to obtain defect-rich powdery carbon precursor sample A;

4) The carbon precursor A was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous and defect-rich carbon powder material B;

5) Put 0.5 g of porous defect carbon powder B in an agate mortar, add 2 ml of ethanol, stir for 30 minutes to disperse the carbon powder in the ethanol evenly, then add 1.0 ml of polyethylene glycol (PEG400) to fully Stir to make the powder carbon into a jelly, put it into the tablet tank of the tablet press (cylindrical type, tablet type) or a pressure-resistant mold, dry it in an environment of 80°C for 24 hours, and finally put it under a pressure of 10MPa Press molding to make the carbon carrier of the microbial water purifying agent.

(2) Add 10 g of defect-rich carbon carriers to 100 ml of Luria-Bertani medium, and add Bacillus licheniformis , Rhodopseudomonas palustris , Chlorella vulgaris , Streptococcus , sulfur oxide The bacillus Thiobacillus Thiooxidant was inoculated into Luria-Bertani medium at a mass ratio of 4:5:1:4:1, the inoculation amount of the compound strain was 2%, and the co-cultivation was shaken at 4°C and 150 rpm for 60 hours, and finally the carbon The carrier is dried in a constant temperature box at 4°C to obtain a water purifier; the effective number of viable bacteria on the carbon carrier is 1×10 ⁹ CFU/g, and the bacterial carbon binding rate reaches 97.4%; the net The water agent can be placed in the sewage directly for 10 hours at room temperature to recover its biological activity before use.

Example 3

A kind of preparation method of the water purifying agent of rich defect carbon carrier immobilized microorganism, concrete steps are:

(1) Preparation of carbon supports with hydrophilic functional groups and defects on the surface

1) Put NaOH+KOH=90g (NaOH:KOH=46.5:43.5, mass ratio) in a 200 ml reactor liner and melt at 200°C for 2 hours to obtain clarified molten lye;

2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 8 hours to generate defect-rich powder carbon precursor material A;

3) Wash the above-mentioned carbon precursor A with water until the pH value of the washing solution is about 7, remove water-soluble impurities, and then centrifuge to obtain the lower precipitate; the lower precipitate is then washed with absolute ethanol to remove water-insoluble organic impurities, and finally centrifuged The precipitate was dried overnight at 80°C to obtain defect-rich powdery carbon precursor sample A;

4) The carbon precursor A was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous and defect-rich carbon material B;

5) Put 1.0 g of porous defective carbon powder B in an agate mortar, add 4 ml of ethanol, stir for 30 minutes to disperse the carbon powder in the ethanol evenly, then add 2 ml of polyethylene glycol (PEG400), fully Stir to make the powder carbon into a jelly, then put it into the tablet tank of the tablet press (cylindrical type, tablet type) or a pressure-resistant mold, dry it in an environment of 80°C for 24 hours, and finally put it under a pressure of 15MPa Press molding to make the carbon carrier of the microbial water purifying agent.

(2) Add 10 g of defect-rich carbon carriers to 50 ml of Luria-Bertani medium, and add Bacillus licheniformis , Rhodopseudomonas palustris , Chlorella vulgaris , Streptococcus , sulfur oxide The bacillus Thiobacillus Thiooxidant was inoculated into Luria-Bertani medium at a mass ratio of 4:5:1:4:1, the inoculation amount of the composite strain was 1%, and the co-cultivation was shaken at 4°C and 150 rpm for 48 hours, and finally the carbon was removed. The carrier is dried in a constant temperature box at 4°C to obtain a water purifier; the effective number of viable bacteria on the carbon carrier is 7×10 ⁸ CFU/g, and the bacterial carbon binding rate reaches 97.1%; the net The water agent can be placed in the sewage directly for 10 hours at room temperature to recover its biological activity before use.

Comparative example 1

Basically the same as in Example 1-3, the water purifying agent for immobilizing microorganisms with a rich and defective carbon carrier is prepared, the only difference is that the carbon carrier prepared in the first three steps (1) is different, and the column type sold in the market is used in Comparative Example 1 Activated carbon support (specific surface about 1100m ² /g, pore volume 0.75 cm ³ /g).

Performance Testing:

1. Put activated carbon carriers of different shapes into the aquaculture anaerobic sewage, and compare their adsorption effects. Weigh 100g of activated carbon carriers of various shapes (i.e. granular coal-based activated carbon A, spherical coal-based activated carbon B, cylindrical coal-based activated carbon C, and defect-rich columnar carbon D), and put them into 10L of aquaculture anaerobic sewage. The untreated aquaculture anaerobic sewage was used as a control, and its CODcr, BOD ₅ , ammonia nitrogen, total phosphorus and other technical indicators were measured after 3.5 days, and the adsorption rate of activated carbon to each index in the aquaculture anaerobic sewage was calculated.

The experimental results are shown in Table 1-1. It can be seen from the experimental results that among the first three, the cylindrical coal-based activated carbon has the better adsorption capacity to the aquaculture anaerobic sewage, and its COD, BOD ₅ , ammonia nitrogen, total phosphorus and other adsorption rates are higher than the first two; The adsorption effect of defective columnar carbon is better than that of A, B, and C, and the adsorption rates of COD, BOD ₅ , ammonia nitrogen, and total phosphorus are all significantly improved. It can be seen that the surface oxygen-containing functional groups and surface defects synergistically promote the adsorption of pollutants. The decontamination effect is better than that of commercially available activated carbon of different shapes.

Table 1-1 The effect of different shapes of carbon carriers on the treatment of aquaculture anaerobic sewage

Two, take five aquaculture anaerobic sewage tanks (10 L) with exactly the same size and water quality for testing, the first puts the microbial water purifier of embodiment 1; the second puts the microbial water purifier of embodiment 2; the second Three of them were injected with the microbial water purifier of Example 3; the fourth was injected with the bacterial carbon water purifier of Comparative Example 1; the fifth was not injected with any water purifier, which was used as a blank control group.

100 g of water purifiers were put into the aquaculture anaerobic sewage tanks, and after 3.5 days, the water quality of all the aquaculture anaerobic sewage tanks was measured. The basis of water quality measurement is GB11607-89 National Standard Fishery Water Quality Standard of the People's Republic of China and NY5051-2001 Water Quality of Pollution-free Food Freshwater Aquaculture Water. The measurement indicators and data are shown in Table 1-2 and Table 1-3 below.

Table 1-2 The effect of different bacterial carbon purifiers on the concentration of sewage indicators after 3.5 days

Table 1-3 The removal effect of different bacterial carbon purifiers on pollutants after 3.5 days

It can be seen from the data in the table that the various water quality indicators of Examples 1-3 are better than those of Comparative Example 1, and even more obviously better than that of the control group.

The above is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, and any person skilled in the art may make changes or modifications without creative work within the technical scope disclosed in the present invention. Replacement should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the protection scope defined in the claims.

Claims (5)

1. A water purifying agent rich in defective carbon carrier immobilizing microorganisms, characterized in that: the rich defective carbon carrier is placed in the Luria-Bertani substratum, and the compound bacterial strain is inoculated to the Luria-Bertani substratum simultaneously, through shaking co-cultivation, After drying, the water purifying agent is obtained; the preparation method of the defect-rich carbon carrier comprises the following steps: (1) Put 90g of mixed alkali of NaOH and KOH in a 200 ml reactor liner, and melt it at 165°C for 2 hours to obtain a clarified molten alkali; (2) Using glucose and urea as raw materials, add them to clarified molten lye and react for 12 hours to generate defect-rich powder carbon precursor materials; (3) After the carbon precursor material was washed with water and absolute ethanol in sequence, it was dried at 80°C for 12 hours to obtain a powder carbon precursor; (4) The powdered carbon precursor was calcined in a muffle furnace at 300°C for 2 hours to obtain a porous defect-rich carbon powder material with a total pore volume of 0.05-0.06 cm ³ /g; (5) Disperse the porous defect-rich carbon powder material in ethanol, then drop polyethylene glycol into the dispersion liquid, stir well to form a jelly, and press it to make the carbon carrier of the microbial water purifier. 2. The water purifying agent for immobilizing microorganisms on rich and defective carbon carriers according to claim 1, characterized in that: the formula of the Luria-Bertani medium is: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000g mL, pH 7.0. 3 . The water purifier for immobilizing microorganisms with defect-rich carbon carriers according to claim 1 , wherein the mass ratio of NaOH to KOH in the mixed alkali described in step (1) is 46.5:43.5. 4. A method for preparing a water purifying agent for immobilizing microorganisms with rich defective carbon carriers as claimed in any one of claims 1-3, characterized in that: 10g rich defective carbon carriers are added to 50-150 ml Luria-Bertani for cultivation In the base, Bacillus licheniformis , Rhodopseudomonas palustris , Chlorella vulgaris , Streptococcus , Thiobacillus Thiooxidant were inoculated at a mass ratio of 4:5:1:4:1 Into the Luria-Bertani medium, the inoculum amount of the compound strain is 1-2%, shake culture at 4°C and 150rpm for 48-72h, and finally remove the carbon carrier and place it in a constant temperature box at 4°C to dry it. Water purifying agent; the effective number of viable bacteria on the carbon carrier is 7×10 ⁸ ~6×10 ⁹ CFU/g. 5. The application of a water purifying agent for immobilizing microorganisms on a defect-rich carbon carrier as described in any one of claims 1-3 in the treatment of aquaculture anaerobic sewage.