CN107432359A - A kind of preparation method of prebiotics ginger sugar - Google Patents
A kind of preparation method of prebiotics ginger sugar Download PDFInfo
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- CN107432359A CN107432359A CN201710744920.8A CN201710744920A CN107432359A CN 107432359 A CN107432359 A CN 107432359A CN 201710744920 A CN201710744920 A CN 201710744920A CN 107432359 A CN107432359 A CN 107432359A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/38—Sucrose-free products
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Abstract
The invention belongs to food processing field, and in particular to a kind of preparation method of prebiotics ginger sugar, mainly using sucrose and ginger as raw material, its preparation method includes the expansion culture of Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma；The preparation of sucrose ginger liquid；Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma are activated and be transferred in sucrose ginger liquid；Filtered and sterilized by plate and frame filter press；Concentration packaging.For the present invention using multiple-microorganism fermentation ginger sugar liquid, cellulose, starch in ginger produce cellulase by trichoderma respectively and Sai Shi aspergillus produces Amylase Hydrolysis, discharges the more active ingredients of ginger, avoids the loss of active ingredient in traditional decoction method；Sucrose changes into FOS by microorganism fermentation of Aspergillus niger, by protaminobacter ruber microbe conversion into palatinose, thus has the effect of prebiotics；Meanwhile the present invention is produced to human body beneficial to ferment class material in fermentation process, adds product function feature and application.
Description
Technical field
The invention belongs to food processing technology field, and in particular to a kind of preparation method of prebiotics ginger sugar.
Background technology
Ginger (Classification system Zingiber officinale Roscoe), is the fresh of Zingiber herbaceos perennial ginger
Rhizome, alias, which has race, hundred peppery clouds, hooks dress refers to, because ground is pungent, hot and cold boy, fresh ginger, honey process ginger.Rhizome (rhizoma zingiberis), the bolt of ginger
Skin (ginger peel), leaf (ginger leaves) can be used as medicine.Ginger has diverging, preventing or arresting vomiting, cough-relieving and other effects in traditional Chinese medicine and pharmacy.CORTEX ZINGIBERIS RHIZOMAE is pungent
It is cool, control skin edema, the row severe edema due to hypofunction of the spleen；Ginger juice pungent-warm, pungent scattered stomach cold strength is strong, is used for vomitting；Rhizoma zingiberis pungent-warm, middle benefit gas are instigated
Cold, Back to Yang Tongmai, warm malaria strength is big, and baked ginger acrid flavour hardship does not walk table in walking, and temperature is lower burnt cold；Baked ginger charcoal is warm-natured, relatively homoiothermy
/ cold；The bitter temperature of ginger is simmered, relatively warming intestines and stomach is cold.The pungent and scattered temperature of ginger, Energy benefit taste, it is apt to middle benefit gas stopping nausea and vomiting by lowering the adverse flow of QI, dehumidify the ruffian that disappears, only
Cough eliminating the phlegm, using stopping nausea and vomiting by lowering the adverse flow of QI as length.Therefore, researcher suggests, usually to have some the food containing ginger more, can be to a certain extent
Ensure the health of people.Ginger sugar is the tasty Han nationality's famous cake of delicious, rises in southern china earliest, is to refine ginger with ginger
Juice and brown sugar are mixed.With the raising that people's health is realized, ginger sugar, which makes, to be increasingly able to finely, and ginger sugar becomes people's happiness
The health food of love.But presently commercially available ginger sugar, the loss of active ingredient is caused using decoction method, while mouthfeel is single, work(
Effect shows slightly single.
The content of the invention
It is an object of the present invention to for the problem of above-mentioned ginger sugar nutritional ingredient is few, effect is single, there is provided one kind can make ginger
The fully prebiotics ginger sugar of release active ingredient.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of preparation method of prebiotics ginger sugar, is mainly made up of the raw material of following parts by weight：Sucrose 65-75 parts and ginger 25-35
Part, its preparation method comprises the following steps：
（1）Expand culture transformed bacteria：Expand culture Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma respectively, it is standby；
（2）It is prepared by raw material：By weight, claim ginger and clean, defibrination is used after it is crushed into 2-5mm particle with Chinese medicine crusher
Machine is milled to 60-100 mesh, obtains ginger liquid；Then sucrose is weighed by weight, and is added into ginger liquid, obtains system, then toward body
SystemIn be slowly added into pure water, while stir until obtaining the sucrose ginger liquid that solid content is 48-55%, this is system；
（3）Conversion：Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma, wherein Sai Shi aspergillus, red protamin bar are taken respectively
The amount of bacterium, aspergillus niger and trichoderma is respectively step（2）Obtained system1-1.5%, 0.5-1%, 1.5-2% and 0.5- of quality
1%, it is placed in after above strain is mixed in activating solution and carries out activation 10-15min, obtain activated spawn, then turns activated spawn
It is connected to step（2）8-12h is converted in obtained sucrose ginger liquid, obtained double prebioticses and the mixing conversion fluid of ginger zymolyte, this
For system；
（4）Filter sterilization：By step（3）Obtained systemFiltered by plate and frame filter press, its pressure is during filtering
0.4-0.45Mpa, obtain the filtrate of light transmittance >=80%, then by filtrate steam pressure be 0.45-0.55Mpa, temperature be
Sterilization 6-8s is carried out under conditions of 116-128 DEG C；Bacteria-free filtrate is obtained, this is system；
（5）Concentration packaging：By step（4）Obtained system25-40s, obtained concentrate are concentrated with vacuum scraper inspissator
As prebiotics ginger sugar, is finally packed.
In the present invention, further instruction, step（1）Described in the expansion cultural method of Sai Shi aspergillus be：By quality
Percentage, sucrose 10-12%, soluble starch 5-8%, soy meal 3-5%, potassium dihydrogen phosphate 0.12-0.15%, yeast is taken to carry respectively
Thing 1-2% and water 70-85% are taken, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains mixed liquor；Then will be mixed
Close under conditions of liquid is placed in 121-125 DEG C and carry out sterilizing 60-90min, it is cooled to 40-42 DEG C after taking-up, obtain culture medium；
Then Sai Shi aspergillus is inoculated in culture medium, its inoculum concentration is 1-3% by mass percentage, and its throughput is 4-8L/ during culture
Min, it is centrifuged after cultivating 36-44h, taking precipitate, sediment is carried out into washing obtains Sai Shi Aspergillus 2-5 times
Body.
In the present invention, further instruction, step（1）Described in the expansion cultural method of protaminobacter ruber be：Press
Mass percent, sucrose 5-8%, phytone 2-4%, yeast extract 1-1.5%, beef extract 1-2%, beancake powder 2- are taken respectively
4%th, disodium hydrogen phosphate 0.12-0.15% and water 80-95%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains
Mixed liquor；Then sterilize 60-90min under conditions of mixed liquor being placed in into 121-125 DEG C, it is cooled to 33-35 DEG C after taking-up,
Culture medium is obtained, protaminobacter ruber is then inoculated in culture medium, its inoculum concentration is 2-2.5% by mass percentage, during culture
Its throughput is 3-5L/min, is centrifuged after cultivating 48-56h, taking precipitate, and washing is carried out to sediment and is obtained for 2-5 times
Protaminobacter ruber thalline.
In the present invention, further instruction, step（1）Described in the expansion cultural method of aspergillus niger be：By quality hundred
Divide ratio, take sucrose 15-18%, corn steep liquor 2-3%, bean cake powder 6-10%, potassium dihydrogen phosphate 0.2-0.3%, magnesium sulfate 0.2- respectively
0.4% and water 72-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains mixed liquor；Then by mixed liquor
Sterilizing 60-90min is carried out under conditions of being placed in 121-125 DEG C, it is cooled to 28-32 DEG C after taking-up, obtains culture medium, then
Aspergillus niger is inoculated in culture medium, its inoculum concentration is 1-2% by mass percentage, and its throughput is 6-9L/min during culture, culture
Centrifuged after 40-48h, taking precipitate, washing is carried out to sediment and produces aspergillus strain 2-5 times.
In the present invention, further instruction, step（1）Described in the expansion cultural method of trichoderma be：By quality percentage
Than taking sucrose 8-11%, soy meal 3-5%, corn flour 1-3%, potassium dihydrogen phosphate 0.15-0.25%, wood chip end 2-3% and water respectively
75-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains mixed liquor；Then mixed liquor is placed in 121-
Sterilize 60-90min under conditions of 125 DEG C, it is cooled to 30-33 DEG C after taking-up, obtains culture medium, is then inoculated in trichoderma
Culture medium, its inoculum concentration are 1-2% by mass percentage, and its throughput is 5-7L/min during culture, and culture culture 50-56h is carried out
Centrifugation, taking precipitate, washing is carried out to sediment and obtains Trichoderma body 2-5 times.
In the present invention, further instruction, step（3）Described in double prebioticses be FOS and palatinose.
In the present invention, further instruction, step（3）Described activating solution is 5-8% aqueous sucrose solutions, and its volume accounts for
SystemThe 1-2% of volume.
In the present invention, further instruction, step（5）Described in concentrate, its concentration condition is：85-105 DEG C of temperature,
Vacuum bears 0.07- and bears 0.08Mpa.
The preparation method of the yeast extract comprises the following steps：
（1）Yeast emulsion is placed in self-dissolving tank, and is heated to 60-70 DEG C, yeast emulsion is maintained under conditions of 60-70 DEG C
Self-dissolving 8-10h, obtains system；
（2）Regulating step（1）Obtained systemPH to 6-7, be subsequently added into glusulase and 6-8h be hydrolyzed, obtain system,
Wherein, the addition of glusulase is 0.20-0.25g in every 1L yeast emulsions；
（3）Toward step（2）In obtained system8-10h is hydrolyzed in middle addition ficin and bromelain, obtains
To system, wherein, the addition of ficin and bromelain is 0.20-0.25g in 1L yeast emulsions；
（4）By step（3）Obtained system80-85 DEG C is heated to, makes systemContinue 40- under conditions of 80-85 DEG C
60min, then it is centrifuged, take supernatant, then supernatant is spray-dried, produce yeast extract.
The preparation method of the yeast extract comprises the following steps：
（1）Yeast emulsion is placed in self-dissolving tank, and is heated to 60-70 DEG C, yeast emulsion is maintained under conditions of 60-70 DEG C
Self-dissolving 8-10h, obtains system；
（2）Regulating step（1）Obtained systemPH to 6-7, be subsequently added into glusulase and 6-8h be hydrolyzed, obtain system,
Wherein, the addition of glusulase is 0.20-0.25g in every 1L yeast emulsions；
（3）Toward step（2）In obtained system8-10h is hydrolyzed in middle addition ficin and bromelain, obtains
To system, wherein, the addition of ficin and bromelain is 0.20-0.25g in 1L yeast emulsions；
（4）By step（3）Obtained system80-85 DEG C is heated to, makes systemContinue 40- under conditions of 80-85 DEG C
60min, then it is centrifuged, take supernatant；
（5）By step（4）The condition that obtained supernatant is placed in less than 60 DEG C is concentrated under reduced pressure, and obtains solid matter weight content and is
28%-32% primary concentrate；Then condition of the primary concentrate below 55 DEG C is concentrated under reduced pressure, that is, obtains solid matter weight
Measure the yeast extract that content is 60%-70%.
The present invention has the advantage that compared with prior art：
1. the effect of preparation method of the present invention can make ginger is not fully exerted, the health care also with prebiotics effect and ferment
Effect.The present invention is produced using ginger and sucrose as raw material by one-step fermentation, using multiple-microorganism fermentation ginger sugar liquid, in ginger
Cellulose, starch produce cellulase and Sai Shi aspergillus by trichoderma respectively and produce Amylase Hydrolysis, discharge ginger it is more effectively into
Point, avoid the loss of active ingredient in traditional decoction method.Sucrose changes into FOS, quilt by microorganism fermentation of Aspergillus niger
Protaminobacter ruber microbe conversion is into palatinose, and FOS is to the profitable strain in enteron aisle such as Bifidobacterium Bifidum, lactic acid bar
The selective proliferation function such as bacterium, makes profitable strain be taken advantage in enteron aisle, suppresses the growth of harmful bacteria, meanwhile, oligomeric fruit
Sugar can only be absorbed by enteric bacteria due to can not directly be digested and assimilated by human body, therefore its calorific value is low, will not cause obesity,
Play a part of fat-reducing indirectly；Palatinose is a kind of and the strong carbohydrate of disaccharides substance decomposition enzyme compatibility, can stabilize sugarcane
Influence of the disaccharides such as sugar, maltose to blood glucose fluctuation, rise so as to suppress blood glucose, while play the role of to suppress accumulation of fat, pa
Lashing wire is sugared or a kind of excellent Bifidobacterium increment factor, though can not by the enzyme system of human body and most microorganisms profit
With, but utilization can be decomposed by the Bifidobacterium in human body intestinal canal, promote the growth and breeding of Bifidobacterium, maintain enteron aisle
Microecological balance, be advantageous to the health of human body.Meanwhile the present invention is produced to human body beneficial to ferment class in fermentation process
Material, add product function feature and application.
2. the present invention is with the product of the raw material production high nutritive value of cheap and simple, more effects, preparation method is simple, tool
There is great promotional value.
Embodiment
The invention provides providing a kind of preparation method of prebiotics ginger sugar, for make the purpose of the present invention, technical scheme and
Effect is clearer, clear and definite, and the present invention is described in more detail below.It should be appreciated that specific embodiment described herein
Only to explain the present invention, it is not intended to limit the present invention.
In following examples, Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma are enlarged training by the following method
Support,
The expansion cultural method of Sai Shi aspergillus is：
By mass percentage, sucrose 10-12%, soluble starch 5-8%, soy meal 3-5%, potassium dihydrogen phosphate 0.12- are taken respectively
0.15%th, yeast extract 1-2% and water 70-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, is mixed
Close liquid；Then sterilizing 60-90min is carried out under conditions of mixed liquor being placed in into 121-125 DEG C, it is cooled to 40-42 after taking-up
DEG C, obtain culture medium；Then Sai Shi aspergillus is inoculated in culture medium, its inoculum concentration is 1-3% by mass percentage, during culture its
Throughput is 4-8L/min, it is centrifuged after cultivating 36-44h, taking precipitate, and sediment is carried out into washing produces for 2-5 times
To Sai Shi aspergillus thalline.
The expansion cultural method of protaminobacter ruber is：
By mass percentage, sucrose 5-8%, phytone 2-4%, yeast extract 1-1.5%, beef extract 1-2%, soya-bean cake are taken respectively
Powder 2-4%, disodium hydrogen phosphate 0.12-0.15% and water 80-95%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content,
Obtain mixed liquor；Then sterilize 60-90min under conditions of mixed liquor being placed in into 121-125 DEG C, it is cooled to 33- after taking-up
35 DEG C, culture medium being obtained, protaminobacter ruber is then inoculated in culture medium, its inoculum concentration is 2-2.5% by mass percentage,
Its throughput is 3-5L/min during culture, is centrifuged after cultivating 48-56h, taking precipitate, and washing 2-5 times is carried out to sediment
Obtain protaminobacter ruber thalline.
The expansion cultural method of aspergillus niger is：
By mass percentage, take respectively sucrose 15-18%, corn steep liquor 2-3%, bean cake powder 6-10%, potassium dihydrogen phosphate 0.2-0.3%,
Magnesium sulfate 0.2-0.4% and water 72-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains mixed liquor；Connect
And sterilizing 60-90min is carried out under conditions of mixed liquor is placed in into 121-125 DEG C, it is cooled to 28-32 DEG C after taking-up, obtain
Culture medium, aspergillus niger is then inoculated in culture medium, its inoculum concentration is 1-2% by mass percentage, and its throughput is 6- during culture
9L/min, centrifuged after cultivating 40-48h, taking precipitate, washing is carried out to sediment and produces aspergillus strain 2-5 times.
The expansion cultural method of trichoderma is：
By mass percentage, take respectively sucrose 8-11%, soy meal 3-5%, corn flour 1-3%, potassium dihydrogen phosphate 0.15-0.25%,
Wood chip end 2-3% and water 75-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains mixed liquor；Then will
Mixed liquor sterilizes 60-90min under conditions of being placed in 121-125 DEG C, it is cooled to 30-33 DEG C after taking-up, obtains culture medium, connects
And trichoderma is inoculated in culture medium, its inoculum concentration is 1-2% by mass percentage, and its throughput is 5-7L/min during culture, culture
Culture 50-56h is centrifuged, taking precipitate, and washing is carried out to sediment and obtains Trichoderma body 2-5 times.
Embodiment 1
A kind of preparation method of prebiotics ginger sugar provided by the invention：
A kind of preparation method of prebiotics ginger sugar, is mainly made up of the raw material of following parts by weight：25 parts of 65 parts of sucrose and ginger, it is made
Preparation Method comprises the following steps：
（1）Expand culture transformed bacteria：Expand culture Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma respectively, it is standby；
（2）It is prepared by raw material：By weight, claim ginger and clean, defibrination is used after it is crushed into 2-5mm particle with Chinese medicine crusher
Machine is milled to 60 mesh, obtains ginger liquid；Then sucrose is weighed by weight, and is added into ginger liquid, obtains system, then toward system
In be slowly added into pure water, while stir until obtaining the sucrose ginger liquid that solid content is 48%, this is system；
（3）Conversion：Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma, wherein Sai Shi aspergillus, red protamin bar are taken respectively
The amount of bacterium, aspergillus niger and trichoderma is respectively step（2）Obtained system1%, 0.5%, 1.5% and the 0.5% of quality, by above bacterium
It is placed in after kind mixing in 5% aqueous sucrose solution, wherein, the volume of 5% aqueous sucrose solution accounts for systemThe 1% of volume, is activated
Activated spawn is obtained after 10min, activated spawn is then transferred to step（2）8h is converted in obtained sucrose ginger liquid, is obtained
The mixing conversion fluid of FOS, palatinose and ginger zymolyte, this is system；
（4）Filter sterilization：By step（3）Obtained systemFiltered by plate and frame filter press, during filtering, its pressure is
0.4Mpa, the filtrate of light transmittance >=80% is obtained, then by filtrate in the condition that steam pressure is 0.45Mpa, temperature is 116 DEG C
Under carry out sterilization 6s；Bacteria-free filtrate is obtained, this is system；
（5）Concentration packaging：By step（4）Obtained systemWith vacuum scraper inspissator temperature be 85 DEG C, vacuum be negative
Concentration 25s is carried out under conditions of 0.078Mpa, obtained concentrate is prebiotics ginger sugar, is finally packed.
Embodiment 2
A kind of preparation method of prebiotics ginger sugar provided by the invention：
A kind of preparation method of prebiotics ginger sugar, is mainly made up of the raw material of following parts by weight：30 parts of 70 parts of sucrose and ginger, it is made
Preparation Method comprises the following steps：
（1）Expand culture transformed bacteria：Expand culture Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma respectively, it is standby；
（2）It is prepared by raw material：By weight, claim ginger and clean, defibrination is used after it is crushed into 2-5mm particle with Chinese medicine crusher
Machine is milled to 80 mesh, obtains ginger liquid；Then sucrose is weighed by weight, and is added into ginger liquid, obtains system, then toward system
In be slowly added into pure water, while stir until obtaining the sucrose ginger liquid that solid content is 51%, this is system；
（3）Conversion：Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma, wherein Sai Shi aspergillus, red protamin bar are taken respectively
The amount of bacterium, aspergillus niger and trichoderma is respectively step（2）Obtained system1.2%, 0.8%, 1.8% and the 0.8% of quality, by more than
It is placed in after strain mixing in 6% aqueous sucrose solution, wherein, the volume of 6% aqueous sucrose solution accounts for systemThe 1.5% of volume, is lived
Activated spawn is obtained after changing 13min, activated spawn is then transferred to step（2）10h is converted in obtained sucrose ginger liquid, is obtained
FOS, the mixing conversion fluid of palatinose and ginger zymolyte, this is system；
（4）Filter sterilization：By step（3）Obtained systemFiltered by plate and frame filter press, during filtering, its pressure is
0.3Mpa, the filtrate of light transmittance >=80% is obtained, then by filtrate under conditions of steam pressure is 0.5Mpa, temperature is 120 DEG C
Carry out sterilization 7s；Bacteria-free filtrate is obtained, this is system；
（5）Concentration packaging：By step（4）Obtained systemWith vacuum scraper inspissator temperature be 95 DEG C, vacuum be negative
Concentration 32s is carried out under conditions of 0.075Mpa, obtained concentrate is prebiotics ginger sugar, is finally packed.
Embodiment 3
A kind of preparation method of prebiotics ginger sugar provided by the invention：
A kind of preparation method of prebiotics ginger sugar, is mainly made up of the raw material of following parts by weight：35 parts of 75 parts of sucrose and ginger, it is made
Preparation Method comprises the following steps：
（1）Expand culture transformed bacteria：Expand culture Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma respectively, it is standby；
（2）It is prepared by raw material：By weight, claim ginger and clean, defibrination is used after it is crushed into 2-5mm particle with Chinese medicine crusher
Machine is milled to 100 mesh, obtains ginger liquid；Then sucrose is weighed by weight, and is added into ginger liquid, obtains system, then toward systemIn be slowly added into pure water, while stir until obtaining the sucrose ginger liquid that solid content is 55%, this is system；
（3）Conversion：Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma, wherein Sai Shi aspergillus, red protamin bar are taken respectively
The amount of bacterium, aspergillus niger and trichoderma is respectively step（2）Obtained system1.5%, 1%, 2% and the 1% of quality, above strain is mixed
It is placed in after conjunction in 8% aqueous sucrose solution, wherein, the volume of 8% aqueous sucrose solution accounts for systemThe 2% of volume, carry out activation 15min
After obtain activated spawn, activated spawn is then transferred to step（2）12h is converted in obtained sucrose ginger liquid, what is obtained is oligomeric
The mixing conversion fluid of fructose, palatinose and ginger zymolyte, this is system；
（4）Filter sterilization：By step（3）Obtained systemFiltered by plate and frame filter press, during filtering, its pressure is
0.45Mpa, the filtrate of light transmittance >=80% is obtained, then by filtrate in the condition that steam pressure is 0.55Mpa, temperature is 128 DEG C
Under carry out sterilization 8s；Bacteria-free filtrate is obtained, this is system；
（5）Concentration packaging：By step（4）Obtained systemWith vacuum scraper inspissator temperature be 105 DEG C, vacuum be negative
Concentration 40s is carried out under conditions of 0.08Mpa, obtained concentrate is prebiotics ginger sugar, is finally packed.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (8)
1. a kind of preparation method of prebiotics ginger sugar, it is characterised in that be mainly made up of the raw material of following parts by weight：Sucrose 65-
75 parts and ginger 25-35 parts, its preparation method comprise the following steps：
（1）Expand culture transformed bacteria：Expand culture Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma respectively, it is standby；
（2）It is prepared by raw material：By weight, claim ginger and clean, defibrination is used after it is crushed into 2-5mm particle with Chinese medicine crusher
Machine is milled to 60-100 mesh, obtains ginger liquid；Then sucrose is weighed by weight, and is added into ginger liquid, obtains system, then it is past
SystemIn be slowly added into pure water, while stir until obtaining the sucrose ginger liquid that solid content is 48-55%, this is system；
（3）Conversion：Sai Shi aspergillus, protaminobacter ruber, aspergillus niger and trichoderma, wherein Sai Shi aspergillus, red protamin bar are taken respectively
The amount of bacterium, aspergillus niger and trichoderma is respectively step（2）Obtained system1-1.5%, 0.5-1%, 1.5-2% and 0.5- of quality
1%, it is placed in after above strain is mixed in activating solution and carries out activation 10-15min, obtain activated spawn, then turns activated spawn
It is connected to step（2）8-12h is converted in obtained sucrose ginger liquid, obtained double prebioticses and the mixing conversion fluid of ginger zymolyte, this
For system；
（4）Filter sterilization：By step（3）Obtained systemFiltered by plate and frame filter press, its pressure is during filtering
0.4-0.45Mpa, obtain the filtrate of light transmittance >=80%, then by filtrate steam pressure be 0.45-0.55Mpa, temperature be
Sterilization 6-8s is carried out under conditions of 116-128 DEG C；Bacteria-free filtrate is obtained, this is system；
（5）Concentration packaging：By step（4）Obtained system25-40s is concentrated with vacuum scraper inspissator, obtained concentrate is i.e.
For prebiotics ginger sugar, finally packed.
A kind of 2. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（1）Described in match
The expansion cultural method of family name's aspergillus is：By mass percentage, sucrose 10-12%, soluble starch 5-8%, soy meal 3- are taken respectively
5%th, potassium dihydrogen phosphate 0.12-0.15%, yeast extract 1-2% and water 70-85%, above raw material, which are mixed and are sufficiently stirred, to be made
Solid content dissolves, and obtains mixed liquor；Then sterilizing 60-90min is carried out under conditions of mixed liquor being placed in into 121-125 DEG C, is taken out
After it is cooled to 40-42 DEG C, obtain culture medium；Then Sai Shi aspergillus is inoculated in culture medium, its inoculum concentration presses quality percentage
Than for 1-3%, its throughput is 4-8L/min during culture, it is centrifuged after cultivating 36-44h, taking precipitate, by sediment
Wash 2-5 times and obtain Sai Shi aspergillus thalline.
A kind of 3. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（1）Described in it is red
The expansion cultural method of color protamin bacillus is：By mass percentage, sucrose 5-8%, phytone 2-4%, yeast leaching are taken respectively
Cream 1-1.5%, beef extract 1-2%, beancake powder 2-4%, disodium hydrogen phosphate 0.12-0.15% and water 80-95%, above raw material are mixed
Merging, which is sufficiently stirred, dissolves solid content, obtains mixed liquor；Then sterilize 60- under conditions of mixed liquor being placed in into 121-125 DEG C
90min, it is cooled to 33-35 DEG C after taking-up, obtain culture medium, protaminobacter ruber is then inoculated in culture medium, it connects
Kind of amount be 2-2.5% by mass percentage, and its throughput is 3-5L/min during culture, is centrifuged after culture 48-56h, takes precipitation
Thing, washing is carried out to sediment and obtains protaminobacter ruber thalline 2-5 times.
A kind of 4. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（1）Described in it is black
The expansion cultural method of aspergillus is：By mass percentage, sucrose 15-18%, corn steep liquor 2-3%, bean cake powder 6-10%, phosphorus are taken respectively
Acid dihydride potassium 0.2-0.3%, magnesium sulfate 0.2-0.4% and water 72-85%, above raw material, which are mixed and are sufficiently stirred, makes solid content
Dissolving, obtains mixed liquor；Then sterilizing 60-90min is carried out under conditions of mixed liquor being placed in into 121-125 DEG C, makes it after taking-up
28-32 DEG C is cooled to, culture medium is obtained, aspergillus niger is then inoculated in culture medium, its inoculum concentration is 1-2% by mass percentage,
Its throughput is 6-9L/min during culture, is centrifuged after cultivating 40-48h, taking precipitate, and washing 2-5 times is carried out to sediment
Produce aspergillus strain.
A kind of 5. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（1）Described in wood
Mould expansion cultural method is：By mass percentage, sucrose 8-11%, soy meal 3-5%, corn flour 1-3%, di(2-ethylhexyl)phosphate are taken respectively
Hydrogen potassium 0.15-0.25%, wood chip end 2-3% and water 75-85%, above raw material, which are mixed and are sufficiently stirred, dissolves solid content, obtains
To mixed liquor；Then sterilize 60-90min under conditions of mixed liquor being placed in into 121-125 DEG C, it is cooled to 30-33 after taking-up
DEG C, culture medium is obtained, trichoderma is then inoculated in culture medium, its inoculum concentration is 1-2% by mass percentage, and it is ventilated during culture
Measure as 5-7L/min, culture culture 50-56h is centrifuged, taking precipitate, and washing is carried out to sediment and obtains trichoderma 2-5 times
Thalline.
A kind of 6. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（3）Described in
Double prebioticses are FOS and palatinose.
A kind of 7. preparation method sugar of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（3）Described
Activating solution is 5-8% aqueous sucrose solutions, and its volume accounts for systemThe 1-2% of volume.
A kind of 8. preparation method of prebiotics ginger sugar according to claim 1, it is characterised in that：Step（5）Described in it is dense
Contracting, its concentration condition are：85-105 DEG C of temperature, vacuum bear 0.07- and bear 0.08Mpa.
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| CN112075523A (en) * | 2020-07-27 | 2020-12-15 | 福建好日子食品有限公司 | Formula and preparation method of edible fermented ginger candy |
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