CN107397042A - A kind of composite type animal enteron aisle regulation preparation and preparation method thereof - Google Patents
A kind of composite type animal enteron aisle regulation preparation and preparation method thereof Download PDFInfo
- Publication number
- CN107397042A CN107397042A CN201710637612.5A CN201710637612A CN107397042A CN 107397042 A CN107397042 A CN 107397042A CN 201710637612 A CN201710637612 A CN 201710637612A CN 107397042 A CN107397042 A CN 107397042A
- Authority
- CN
- China
- Prior art keywords
- preparation
- clostridium butyricum
- enteron aisle
- fermentation
- acidophilus
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 43
- 241001465754 Metazoa Species 0.000 title claims abstract description 28
- 230000033228 biological regulation Effects 0.000 title claims abstract description 13
- 239000002131 composite material Substances 0.000 title claims abstract description 13
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 32
- 239000006041 probiotic Substances 0.000 claims abstract description 21
- 235000018291 probiotics Nutrition 0.000 claims abstract description 21
- 206010012735 Diarrhoea Diseases 0.000 claims abstract description 19
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 18
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 18
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 15
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims abstract description 11
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 9
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 9
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 238000013329 compounding Methods 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 18
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 235000015278 beef Nutrition 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
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- 235000018417 cysteine Nutrition 0.000 claims description 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 6
- 229910052564 epsomite Inorganic materials 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 6
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 6
- 229920000053 polysorbate 80 Polymers 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- 241000165940 Houjia Species 0.000 claims description 3
- 229920001202 Inulin Polymers 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
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- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 3
- 229940029339 inulin Drugs 0.000 claims description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
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- 230000003712 anti-aging effect Effects 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 210000001842 enterocyte Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000004051 gastric juice Anatomy 0.000 description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 2
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- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
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- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
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- 230000015572 biosynthetic process Effects 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
- 150000004652 butanoic acids Chemical class 0.000 description 1
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- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
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- 238000003304 gavage Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
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- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
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- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a kind of composite type animal enteron aisle regulation preparation and preparation method thereof, mainly it is made up of probiotics and prebiotics compounding, the probiotics includes clostridium butyricum and lactobacterium acidophilus, and prebiotics includes lichee polyphenol and glutamine.Compound micro-ecological preparation of the present invention, promote clostridium butyricum and lactobacillus acidophilus in enteron aisle internal breeding using lichee polyphenol and glutamine, the effect of so as to play bidirectional modulation gut flora.It has good stability the compound micro-ecological preparation, can adjust microbial population of animal intestinal tract, improves animal disease resistant ability, reduces diarrhea rate and the death rate, and can keep higher survival rate, is adapted to the demand of feed industry and livestock breeding industry.
Description
Technical field
The invention belongs to additive for microbe feedstuff technical field, and in particular to a kind of composite type animal enteron aisle regulation preparation and its
Preparation method.
Background technology
For a long time, the abuse of antibiotic brings serious threat to the health of animal husbandry and the mankind.Probiotics is made
For a kind of emerging feed addictive and Substitutes For Antibiotic, have and directly fill into exogenous beneficial bacterium, adjust animal itself intestines
Road microecological balance, suppress harmful bacteria, strengthen immunity of organisms, so as to promote healthy growth of animal etc. to act on.It is domestic
Outer scholar has carried out substantial amounts of research to it, and applies in animal-breeding, and many studies and clinical applications are found, probiotics exists
Animal intestinal tract microorganism species balance is adjusted, prevention disease, improves food conversion, improves animal immunizing power, improves animal productiong
Performance and preserve the ecological environment etc. that there are other additives irreplaceable.Therefore Speeding up development, the new nothing of popularization
Public hazards, can the probiotics product of effectively disease preventing and treating can promote aquaculture healthy and sustainable development.
In recent years, in the market is various based on all kinds of feed addictive species for improving animal intestinal tract health, such as probiotics, widow
Sugar, acidulant, butyric acid class and glutamine etc., market are probiotics and synbiotic product of the existing part for animal-breeding,
But granulation or swelling temperature are very high during feed manufacturing, easily cause probiotics to inactivate, probiotic effects are difficult to play, because
This is, it is necessary to screen the bacterial strain that probiotic effects can be also played under the conditions of some can survive or inactivate under the high temperature conditions.Butyric acid shuttle
Bacterium has adjustment intestinal colony balance, promotes intestinal beneficial flora propagation, strengthen immunologic function as a kind of new probiotic bacterium,
The functions such as prebiotic product and growth promotion are produced in enteron aisle, while clostridium butyricum produces endogenous spore, stability is good, can be heat-resisting resistance to
Acid, bile tolerance, do not influenceed by hydrochloric acid in gastric juice, bile acid etc., thus by alimentary canal non-inactivation, can preserve at room temperature in vitro 3 years with
On do not fail, it is with the obvious advantage in Feed Manufacturing is processed and is stored, be the desirable strain of feeding probiotics, and clostridium butyricum pair
Part antibiotic such as penicillin, ampicillin, streptomysin, gentamicin, chloramphenicol etc. have certain resistance, and many experiments are demonstrate,proved
Bright it is without any side effects, has been widely used for the additive and vegetable fertilizer of medicine, functional health-care food and animal feed
Material.
Lactic acid bacteria as microorganisms feed additive be most widely used, one kind that effect is best.China's agricultural
It is largely lactic acid bacteria in the feed level microbe additive that portion announces, it is the main fungal component of many animals alimentary canal, energy
Form normal flora.In the multiple-microorganism of intestines and stomach 400, lactic acid bacteria accounts for 10%, is the Major Members of normal intestinal flora.
Physiological function is mainly shown as known to lactic acid bacteria：Invasion and field planting of the pathogen to enteron aisle are prevented, suppresses pathogen and anti-sense
Dye, the microecological balance of enteron aisle, prevention and the generation for suppressing tumour are maintained, strengthen immunity of organisms, promote digestion, synthesizing amino
Acid and vitamin, cholesterol is reduced, suppresses endotoxic generation, anti-aging and radioresistance etc..Wherein with lactobacillus, bifid
Bacillus is representative, and has been found clinically treat and prevent some intestines problems of humans and animals or play health care
Effect.Lactobacillus acidoilus have the ability of well tolerable hydrochloric acid in gastric juice and drug concentration in bile simultaneously as the most important beneficial bacterium of lactic acid bacteria
Have inhibitory action to pathogenic Escherichia coli, be the good standby bacterial strain of probiotics and be important health promotion because
Son, its special acidophilus performance can preferably help enteron aisle to resist disease, and maintain enteron aisle normal function.
Because plant polyphenol has unique functional activity, it has also become the focus researched and developed now, and produced in reality
In obtained widely applying.Lichee polyphenol not only have general polyphenol characteristic and physiological function, such as it is anti-oxidant, antitumor,
Antiviral, antimicrobial, anti-aging, immunity etc. is improved, and there is special anti-inflammatory and antiallergy and anti-infectious work
With being mainly manifested in by improving intestine immunity ability, it is disorderly to improve function of intestinal canal, so as to improve enteric microorganism profitable strain
Quantity；Anti-oxidation stress, antiallergy；Lichee polyphenol and its metabolin such as some special phenolic acid have the effect of anti-inflammatory,
Poisonous, harmful and stink substance content in animal wastes can be substantially reduced, so as to improve environmental sanitation.Glutamine is small
The main energy sources of enterocyte metabolism.In vivo study shows, while glutamine provides energy to enterocyte, can promote intestines
Road cell repair, intestinal villi recover and maintain normal Gut mucosal immune function, so as to help to maintain the complete of intestinal mucosa
Whole property and be advantageous to fully absorbing for intestinal health and nutriment.Although lichee polyphenol and the wholesome work of glutamine
With well-established by the numerous people, but ground zero is gone back in the research in terms of Animal diseases are prevented.If by probiotics, lichee polyphenol
Optimize with glutamine compatibility, play its coordinative role, there is growth promotion and strengthen endogenous and exogenous probiotic active
Double action, it can effectively strengthen the work of intestine immunity；With significantly anti-oxidation stress and improve function of intestinal canal disorder, increase
Intestinal beneficial flora, it can certainly will well improve intestinal health, reduce intestines problem, its using effect or better than antibiotic
With single antibiotic, while there is not yet the problems such as medicament residue.Develop the compound microecological of feeding probiotics and lichee polyphenol
Preparations have important research and production application value, and it provides a new approaches and new way for the sound development of animal husbandry
Footpath.
The content of the invention
To solve and making up problems of the prior art and defect, the present invention provides a kind of composite type animal enteron aisle and adjusted
Agent and preparation method thereof is controlled, resulting preparation can reduce diarrhea of mouse rate, improve antioxidant ability of organism and promote intestines
The formation of road dominant microflora.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of composite type animal enteron aisle adjusts preparation, is formed by probiotics and prebiotics compounding, the probiotics includes butyric acid shuttle
Bacterium (Clostridium butyricum)With lactobacillus acidophilus (Lactobacillus acidophilus), the prebiotics is
Lichee polyphenol and glutamine.
It is a further object of the invention to provide a kind of preparation method of composite type animal enteron aisle regulation preparation, technical side
Case is as follows：
A kind of preparation method of composite type animal enteron aisle regulation preparation, specific implementation step are as follows：
S1. the culture of clostridium butyricum and lactobacterium acidophilus：Clostridium butyricum and lactobacterium acidophilus' seed are trained from test tube slant respectively
Support in base and be inoculated into the conical flask equipped with fluid nutrient medium, 48h is cultivated at 37 DEG C；
S2. ferment：The clostridium butyricum of logarithmic phase will be in S1 and lactobacterium acidophilus are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature are 37 DEG C, fermentation time 20h, and refrigerated centrifuge collects thalline after fermentation；
S3. raw material mix：By the clostridium butyricum and lactobacterium acidophilus' thalline in mass ratio 1 of gained in S2:1 mixing, Ran Houjia
Enter lichee polyphenol and glutamine mixes, the addition of lichee polyphenol and glutamine is 5wt%；
S4. it is freeze-dried：Continue to add 10wt% skimmed milks, 5wt% trehaloses, 10wt% into raw material of the S3 after well mixed
Maltodextrin and 5wt% inulin are as cryoprotector, pre-freeze after stirring, and it is dry that mixture then is transferred quickly into freezing
Dry machine is freezed, and compound formulation is made.
Further, clostridium butyricum Liquid Culture based formulas is in step S1：Peptone 10.0g, beef extract powder 10.0g,
Cysteine 0.5g, yeast extract 3.0g, glucose 5.0g, CH3COONa·3H2O 3.0g, NaCl 5.0g, soluble starch
1.0g and distilled water 1L, pH 6.5-7.0,121 DEG C of autoclaving 15-20min.
Further, lactobacterium acidophilus' Liquid Culture based formulas is in step S1：Peptone 10.0g, beef extract powder 8.0g,
Yeast extract 4.0g, glucose 20.0g, K2HPO42.0g, Na3C6H5O72.0g, CH3COONa·3H2O 5.0g,
MgSO4.7H2O 0.2g, MnSO4·4H2O 0.05g, Tween 80 1ml and distilled water 1L, pH 5.5-6.0,121 DEG C of high pressures are gone out
Bacterium 15-20min.
Further, clostridium butyricum fermentative medium formula is in step S2：Glucose 12.0-15.0g, peptone
12.0-15.0g, yeast extract 10.0-12.0g, beef extract powder 5.0-8.0g, corn flour 5.0-7.0g, cysteine 0.5-
1.0g, NaCl 0.5-2.0g, CH3COONa3H2O 1.0-3.0g, MgSO4·7H2O 1.0-2.0g, (NH4)2SO4 1.0-
2.0g and distilled water IL, pH value 6.8-7.2,121 DEG C of autoclaving 15-20min.
Further, lactobacterium acidophilus' fermentative medium formula is in step S2：Glucose 16.0-20.0g, peptone
10.0-12.0g, yeast extract 6.0-8.0g, corn flour 10.0-12.0g, CH3COONa·3H2O 5.0-7.0g, Na3C6H5O7
2.0-3.0g, MgSO4·7H2O 0.5-1.0g, MnSO4·4H2O 0.5-1.0g, Tween 80 0.5-1.0ml and distilled water 1L,
PH 5.5-6.0,121 DEG C of autoclaving 15-20min.
Further, after fermentation clostridium butyricum bacterial strain concentration up to 107CFU/ml, lactobacterium acidophilus' bacterial strain concentration are reachable
1010CFU/ml, after drying in product viable count up to 109/ gram more than.
Further, centrifugal condition is in step S2：Temperature is 4 DEG C, rotating speed 4000rpm, centrifugation time 5min.
Further, condition is freeze-dried in step S2 is：Pre-freezing temperature is -80 DEG C, pre-freeze time 2h；Cryogenic temperature-
82 DEG C, pressure 3pa.
Third object of the present invention is, there is provided composite type animal enteron aisle adjusts preparation in terms of animal diarrhea preventing and treating
Using.
Compared with prior art, the present invention has the beneficial effect that：
1) clostridium butyricum in the present invention and lactobacterium acidophilus can be carried to greatest extent using the method mixed after separately fermentation
High strain fermentation amount.
2) a variety of probiotics and the efficient compatibility of prebiotics, feature synergy is served, body intestine immunity can be strengthened
Effect, significantly anti-oxidation stress and can improve that function of intestinal canal is disorderly, and increase enteron aisle dominant microflora content and optimization enteron aisle are micro-
Biological flora structure, animal disease resistant ability is improved, reduce diarrhea rate and the death rate.
Brief description of the drawings
Fig. 1 is mouse duodenal histopathology HE stained slice figures.
Specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, but the present invention is not limited in any form,
It is all to do all any modification, equivalent and improvement within spirit and principle, should be included in protection scope of the present invention it
It is interior.Unless stated otherwise, the reagent of the invention used, method and apparatus is the art conventional reagent, methods and apparatus.Remove
Non-specifically illustrate, following examples institute is purchased in market using reagent and material.
A kind of preparation of composite type animal enteron aisle regulation preparation of case study on implementation 1
1.1 materials prepare
(1) bacterial strain
Clostridium butyricum (Clostridium butyricum), deposit number are GIMCC GIM 1.676, lactobacillus acidophilus
(Lactobacillus acidophilus), deposit number are that GIMCC GIM 1.730 are purchased from Guangdong Microbes Inst bacterium
Kind collection.
(2) lichee polyphenol
By being extracted in lychee exocarp, purity is more than 90%.
(3) culture medium
1. lactobacterium acidophilus' Liquid Culture based formulas is：Peptone 10.0g, beef extract powder 8.0g, yeast extract 4.0g, glucose
20.0g K2HPO42.0g, Na3C6H5O72.0g, CH3COONa·3H2O 5.0g, MgSO4.7H2O 0.2g, MnSO4·4H2O
0.05g, Tween 80 1ml and distilled water 1L, pH 5.5-6.0,121 DEG C of autoclaving 15-20min.
2. clostridium enrichment liquid body culture medium：Peptone 10.0g, beef extract powder 10.0g, cysteine 0.5g, yeast extract
3.0g, glucose 5.0g, CH3COONa·3H2O 3.0g, NaCl 5.0g, soluble starch 1.0g and distilled water 1L, pH 6.5-
7.0,121 DEG C of autoclaving 15-20min.
3. lactobacterium acidophilus' fermentation medium：Glucose 16.0-20.0g, peptone 10.0-12.0g, yeast extract 6.0-
8.0g, corn flour 10.0- 12.0g, CH3COONa·3H2O 5.0-7.0g, Na3C6H5O7 2.0-3.0g, MgSO4·7H2O
0.5-1.0g, MnSO4·4H2O 0.5-1.0g, Tween-80 0.5-1.0mL and distilled water 1L, pH 5.5-6.0,121 DEG C of high pressures
Sterilize 15-20min.
4. clostridium butyricum fermentation medium：Glucose 12.0-15.0g, peptone 12.0-15.0g, yeast extract 10.0-
12.0g, beef extract powder 5.0-8.0g, corn flour 5.0-7.0g, cysteine 0.5-1.0g, NaCI 0.5-2g, CH3COONa·
3H2O 1.0-3.0g, MgSO4·7H2O 1.0-2.0g, (NH4)2SO4 1.0-2.0g and distilled water 1L, pH value 6.8-7.2,21
DEG C autoclaving 15-20min.
1.2 preparation method
A kind of preparation method of composite type animal enteron aisle regulation preparation, specific implementation step are as follows：
S1. the culture of clostridium butyricum and lactobacterium acidophilus：Clostridium butyricum and lactobacterium acidophilus' seed are trained from test tube slant respectively
Support in base and be inoculated into the conical flask equipped with fluid nutrient medium, 48h is cultivated at 37 DEG C；
S2. ferment：The clostridium butyricum of logarithmic phase will be in S1 and lactobacterium acidophilus are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature are 37 DEG C, fermentation time 20h, and lactobacillus acidophilus clump count is made as 1010~1011Cfu/mL bacterium
Liquid, clostridium butyricum total plate count are 106~107Cfu/mL bacterium solution, refrigerated centrifuge collects thalline, wherein centrifugal condition after fermentation
For：Temperature is 4 DEG C, rotating speed 4000rpm, centrifugation time 5min, and freeze-drying condition is：Pre-freezing temperature is -80 DEG C, pre-freeze
Time 2h；- 82 DEG C of cryogenic temperature, pressure 3pa；
S3. raw material mix：By the clostridium butyricum and lactobacterium acidophilus' thalline in mass ratio 1 of gained in S2:1 mixing, Ran Houjia
Enter lichee polyphenol and glutamine mixes, the addition of lichee polyphenol and glutamine is 5wt%；
S4. it is freeze-dried：Continue to add 10wt% skimmed milks, 5wt% trehaloses, 10wt% into raw material of the S3 after well mixed
Maltodextrin and 5wt% inulin are as cryoprotector, pre-freeze after stirring, and it is dry that mixture then is transferred quickly into freezing
Dry machine is freezed, and compound formulation is made.
The viable count for measuring compound micro-ecological preparation is 1.52 × 109/ gram.
Application of the compound micro-ecological preparation of case study on implementation 2 in terms of animal diarrhea preventing and treating
2.1 diarrhea of mouse are tested
（1）Experiment process
SPF level kunming mices, after resting 7 days, it is randomly divided into 4 experiment process groups：Model group, clostridium butyricum group, acidophilus lactic acid
Bacterium adjusts preparation group.Experimental period is 14 d, and 400mg/kg is pressed in experiment the 1st d to the 14th d.Bw gives given the test agent, as
Contrast, model group give the physiological saline of equivalent.Castor oil medicine gavage mouse is utilized in the 10th d of experiment, establishes mouse abdomen
Rush down model, measure diarrhoea index.14th d, collection blood separation serum, gathers ileum and aseptic collection excrement.Serum is used to resist
Oxidation index（SOD, GSH-Px and CAT）Measure, ileum are used to make HE stained slices, carry out intestinal tissue morphological observation,
Excrement be used for gut flora PCR-denaturing gradient gel electrophoresis (polymerase chain reaction,
Denaturing gradient gel electrophoresis, PCR-DGGE) detection, intestine microbial diversity observation.
（2）The processing of experimental data
All experimental datas are analyzed using SPSS17.0 statistical softwares.Multigroup is compared using one-way analysis of variance (One-
Way Anova), compare two-by-two using Duncan methods.As a result represented with mean ± standard deviation,p<0.05 is that difference has conspicuousness meaning
Justice.
（3）Experimental result
Mouse test results as shown in table 1 below, table 2, table 3 and table 4, in table the different letter of same row represent significant difference (p<
0.05)：
The diarrhoea index of table 1
| Group | Start the time (min) of suffering from diarrhoea | Loose stools weight (g) | Diarrhoea average time | Loose stool rate (%) | Diarrhoea inhibiting rate (%) |
| Model group | 75.42±8.62a | 0.58±0.06b | 8.62±1.40c | 0.58±0.06b | - |
| Clostridium butyricum group | 84.50±5.03a | 0.53±0.08b | 7.50±1.58 bc | 0.54±0.03b | 13 |
| Lactobacillus acidophilus group | 96.37±8.10b | 0.53±0.03b | 6.55±1.81ab | 0.53±0.09ab | 24 |
| Adjust preparation group | 117.33±15.06c | 0.42±0.42a | 5.55±1.01a | 0.43±0.04a | 36 |
Table 2：Antioxidant Indexes
Table 3：Intestinal tissue morphological index
The intestine microbial diversity index analysis of table 4
| Group | Richness（n） | The uniformity | Shannon indexes |
| Model group | 17.00±2.19a | 0.88±0.02a | 2.59±0.09a |
| Clostridium butyricum group | 21.50±2.43ab | 0.88±0.02ab | 2.68±0.05ab |
| Lactobacillus acidophilus group | 23.67±3.21b | 0.90±0.05 ab | 2.85±0.02 ab |
| Adjust preparation group | Richness（n） | The uniformity | Shannon indexes |
As seen from the above table, regulation preparation embodies obvious anti diar rhea, antioxidant effect to mouse castor oil Diarrhea Model, and imitates
Fruit is better than single use probiotics；Duodenal V/C indexes can be increased, be shown in Table 3, intestines can be promoted to a certain extent
The abilities of digestive and absorption in road；As shown in table 4, flora richness in enteron aisle, the uniformity, Shannon indexes, Neng Gouzeng can be increased
Add the quantity of gut flora, increase the diversity of flora, strengthen the stability of Tiny ecosystem.
From the above results, the present invention is obtained two-way in gut flora by specific prebiotics compatibility probiotics
Regulation aspect has the enteron aisle adjustment preparation of significantly preventing and treating diarrhoea effect.
The enteron aisle regulation preparation of the present invention is embodied in diarrhea of mouse experiment：Diarrhea of mouse rate can effectively be suppressed,
Inhibiting rate suffer from diarrhoea up to 36%, effect is better than single use probiotics；Antioxidant ability of organism can be effectively improved, as SOD in serum,
GSH-Px and CAT vigor；Duodenal V/C indexes are improved, the abilities of digestive and absorption of enteron aisle can be promoted to a certain extent；Increase
Add enteron aisle dominant microflora content and optimization mouse intestinal microbial bacteria group structure.
Claims (10)
1. a kind of composite type animal enteron aisle adjusts preparation, it is characterised in that formed by probiotics and prebiotics compounding, it is described prebiotic
Bacterium bag contains clostridium butyricum and lactobacillus acidophilus, and the prebiotics is lichee polyphenol and glutamine.
2. the preparation method of enteron aisle regulation preparation described in claim 1, it is characterised in that specific implementation step is as follows：
S1. the culture of clostridium butyricum and lactobacterium acidophilus：Clostridium butyricum and lactobacterium acidophilus' seed are trained from test tube slant respectively
Support in base and be inoculated into the conical flask equipped with fluid nutrient medium, 48h is cultivated at 37 DEG C；
S2. ferment：The clostridium butyricum of logarithmic phase will be in S1 and lactobacterium acidophilus are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature are 37 DEG C, fermentation time 20h, and refrigerated centrifuge collects thalline after fermentation；
S3. raw material mix：By the clostridium butyricum and lactobacterium acidophilus' thalline in mass ratio 1 of gained in S2:1 mixing, Ran Houjia
Enter lichee polyphenol and glutamine mixes, the addition of lichee polyphenol and glutamine is 5wt%；
S4. it is freeze-dried：Continue to add 10wt% skimmed milks, 5wt% trehaloses, 10wt% into raw material of the S3 after well mixed
Maltodextrin and 5wt% inulin are as cryoprotector, pre-freeze after stirring, and it is dry that mixture then is transferred quickly into freezing
Dry machine is freezed, and compound formulation is made.
3. preparation method according to claim 2, it is characterised in that clostridium butyricum Liquid Culture based formulas is in step S1：
Peptone 10.0g, beef extract powder 10.0g, cysteine 0.5g, yeast extract 3.0g, glucose 5.0g, CH3COONa·3H2O
3.0g, NaCl 5.0g, soluble starch 1.0g and distilled water 1L, pH 6.5-7.0,121 DEG C of autoclaving 15-20min.
4. preparation method according to claim 2, it is characterised in that lactobacterium acidophilus' Liquid Culture based formulas in step S1
For：Peptone 10.0g, beef extract powder 8.0g, yeast extract 4.0g, glucose 20.0g, K2HPO42.0g, Na3C6H5O7
2.0g, CH3COONa·3H2O 5.0g, MgSO4.7H2O 0.2g, MnSO4·4H2O 0.05g, Tween 80 1ml and distilled water
1L, pH 5.5-6.0,121 DEG C of autoclaving 15-20min.
5. preparation method according to claim 2, it is characterised in that clostridium butyricum fermentative medium formula is in step S2：
Glucose 12.0-15.0g, peptone 12.0-15.0g, yeast extract 10.0-12.0g, beef extract powder 5.0-8.0g, corn flour
5.0-7.0g, cysteine 0.5-1.0g, NaCl 0.5-2.0g, CH3COONa3H2O 1.0-3.0g, MgSO4·7H2O
1.0-2.0g (NH4)2SO41.0-2.0g and distilled water IL, pH value 6.8-7.2,121 DEG C of autoclaving 15-20min.
6. preparation method according to claim 2, it is characterised in that lactobacterium acidophilus' fermentative medium formula in step S2
For：Glucose 16.0-20.0g, peptone 10.0-12.0g, yeast extract 6.0-8.0g, corn flour 10.0-12.0g,
CH3COONa·3H2O 5.0-7.0g, Na3C6H5O72.0-3.0g, MgSO4·7H2O 0.5-1.0g, MnSO4·4H2O 0.5-
1.0g, Tween 80 0.5-1.0ml and distilled water 1L, pH 5.5-6.0,121 DEG C of autoclaving 15-20min.
7. preparation method according to claim 2, it is characterised in that clostridium butyricum bacterial strain concentration is up to 10 after fermentation7CFU/
Ml, lactobacterium acidophilus' bacterial strain concentration is up to 1010CFU/ml, after drying in product viable count up to 109/ gram more than.
8. preparation method according to claim 2, it is characterised in that centrifugal condition is in step S2：Temperature is 4 DEG C, rotating speed
For 4000rpm, centrifugation time 5min.
9. preparation method according to claim 2, it is characterised in that freeze-drying condition is in step S2：Pre-freezing temperature for-
80 DEG C, pre-freeze time 2h；- 82 DEG C of cryogenic temperature, pressure 3pa.
10. application of the composite type animal enteron aisle regulation preparation in terms of animal diarrhea preventing and treating described in claim 1.
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Cited By (9)
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| CN108676739A (en) * | 2018-04-10 | 2018-10-19 | 凯磊（北京）科技有限公司 | A kind of method that clostridium butyricum prepares composite probiotics preparations with lactobacillus acidophilus combined ferment |
| CN109097306A (en) * | 2018-08-30 | 2018-12-28 | 湛江恒兴养殖技术服务有限公司 | Lactic acid bacteria growth-promoting agent and its preparation method and application |
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| CN110353099A (en) * | 2019-09-02 | 2019-10-22 | 河南牧业经济学院 | A kind of production method of clostridium butyricum and clostridium butyricum feed addictive |
| CN111494398A (en) * | 2020-04-02 | 2020-08-07 | 广州大学 | Application of lychee pulp polyphenol QRR and its composition in the preparation of preparations for improving intestinal microecology |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234621A (en) * | 2010-04-21 | 2011-11-09 | 嘉兴活力达生物科技有限公司 | Freeze-drying protective agent for lactic acid bacteria |
| CN103652322A (en) * | 2012-09-21 | 2014-03-26 | 临沂思科生物科技有限公司 | Composite probiotics feed additive containing lactic acid bacteria and preparation method thereof |
| CN105685970A (en) * | 2016-02-01 | 2016-06-22 | 北京东方兴企食品工业技术有限公司 | Compound nutritious food capable of improving whole digestive tract |
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
-
2017
- 2017-07-31 CN CN201710637612.5A patent/CN107397042A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102234621A (en) * | 2010-04-21 | 2011-11-09 | 嘉兴活力达生物科技有限公司 | Freeze-drying protective agent for lactic acid bacteria |
| CN103652322A (en) * | 2012-09-21 | 2014-03-26 | 临沂思科生物科技有限公司 | Composite probiotics feed additive containing lactic acid bacteria and preparation method thereof |
| CN103652322B (en) * | 2012-09-21 | 2016-02-10 | 临沂思科生物科技有限公司 | A kind of preparation method of lactobacteria-containing composite probiotics feed additive |
| CN105685970A (en) * | 2016-02-01 | 2016-06-22 | 北京东方兴企食品工业技术有限公司 | Compound nutritious food capable of improving whole digestive tract |
| CN106479924A (en) * | 2016-10-31 | 2017-03-08 | 湖北绿雪生物科技有限公司 | The preparation of a kind of Clostridium butyricum and clostridium butyricum active bacteria preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| 张军丽等: ""复合菌-荔枝多酚对小鼠抗腹泻效果及抗氧化性的影响"", 《食品工业科技》 * |
| 涂杜: "丁酸梭菌及荔枝多酚联合应用对腹泻小鼠抗氧化功能的影响", 《畜牧与饲料科学》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676739A (en) * | 2018-04-10 | 2018-10-19 | 凯磊（北京）科技有限公司 | A kind of method that clostridium butyricum prepares composite probiotics preparations with lactobacillus acidophilus combined ferment |
| CN108522873A (en) * | 2018-04-20 | 2018-09-14 | 安徽天邦生物技术有限公司 | A kind of lactic acid bacteria and combination of prebiotics product improving piglet growth performance |
| CN109097306A (en) * | 2018-08-30 | 2018-12-28 | 湛江恒兴养殖技术服务有限公司 | Lactic acid bacteria growth-promoting agent and its preparation method and application |
| CN109566893A (en) * | 2019-01-31 | 2019-04-05 | 江苏微康生物科技有限公司 | A kind of preparation method of the probiotic additive applied to livestock-raising |
| CN110353099A (en) * | 2019-09-02 | 2019-10-22 | 河南牧业经济学院 | A kind of production method of clostridium butyricum and clostridium butyricum feed addictive |
| CN111494398A (en) * | 2020-04-02 | 2020-08-07 | 广州大学 | Application of lychee pulp polyphenol QRR and its composition in the preparation of preparations for improving intestinal microecology |
| CN111513202A (en) * | 2020-05-29 | 2020-08-11 | 中农极润生物科技(北京)有限公司 | Microecological preparation and preparation method and application thereof |
| CN114642260A (en) * | 2022-05-24 | 2022-06-21 | 仙乐健康科技股份有限公司 | Litchi ferment, and preparation method and application thereof |
| CN116687979A (en) * | 2023-06-28 | 2023-09-05 | 中国人民解放军陆军军医大学 | Application of intestinal microorganisms in stabilizing intestinal glutamine and preparing medicines for treating cancers |
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