CN107384821A - A kind of fine and close culture medium for Bacteria Culture and preparation method thereof - Google Patents
A kind of fine and close culture medium for Bacteria Culture and preparation method thereof Download PDFInfo
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- 239000001963 growth medium Substances 0.000 title claims abstract description 43
- 241000894006 Bacteria Species 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 238000012136 culture method Methods 0.000 title abstract description 3
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 45
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 38
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 36
- 239000012266 salt solution Substances 0.000 claims abstract description 36
- 229920001817 Agar Polymers 0.000 claims abstract description 35
- 239000008272 agar Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000000284 extract Substances 0.000 claims abstract description 20
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims abstract description 19
- 229940006091 aloe polysaccharide Drugs 0.000 claims abstract description 19
- 235000015278 beef Nutrition 0.000 claims abstract description 19
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 19
- 229960003067 cystine Drugs 0.000 claims abstract description 19
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000203 mixture Substances 0.000 claims abstract description 19
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 19
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 19
- 239000012137 tryptone Substances 0.000 claims abstract description 19
- 239000012138 yeast extract Substances 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 18
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 18
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 18
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims abstract description 18
- 235000019799 monosodium phosphate Nutrition 0.000 claims abstract description 18
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims abstract description 18
- 239000008367 deionised water Substances 0.000 claims abstract description 17
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 8
- 235000010419 agar Nutrition 0.000 claims description 8
- -1 aloe polysaccharide Substances 0.000 claims description 8
- 235000004554 glutamine Nutrition 0.000 claims description 8
- 239000002504 physiological saline solution Substances 0.000 claims description 8
- 241000186000 Bifidobacterium Species 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims 1
- NFIYTPYOYDDLGO-UHFFFAOYSA-N phosphoric acid;sodium Chemical compound [Na].OP(O)(O)=O NFIYTPYOYDDLGO-UHFFFAOYSA-N 0.000 claims 1
- 239000006041 probiotic Substances 0.000 abstract description 14
- 235000018291 probiotics Nutrition 0.000 abstract description 14
- 230000001954 sterilising effect Effects 0.000 abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 7
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 abstract description 2
- 238000012937 correction Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 230000036541 health Effects 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000186660 Lactobacillus Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241001608472 Bifidobacterium longum Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000020167 acidified milk Nutrition 0.000 description 1
- 230000000675 anti-caries Effects 0.000 description 1
- 229940009291 bifidobacterium longum Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of fine and close culture medium for Bacteria Culture and preparation method thereof, is related to bacteria culture media and its preparing technical field, and the fine and close culture medium is made up of the raw material of following mass percent：Tryptone 1.7 1.9%, beef extract 0.5 0.7%, yeast extract powder 0.1 0.2%, aloe polysaccharide 1.7 1.9%, magnesium sulfate 0.1 0.2%, glutamine 0.5 0.6%, 30% yolk salt solution 4.8 5.1%, agar 1.3 1.5%, potassium nitrate 0.2 0.3%, sodium dihydrogen phosphate 0.2 0.3%, cystine 1.1 1.3%, surplus is deionized water；Its preparation is that the composition in addition to 30% yolk salt solution is added in deionized water, and heating is boiled to being completely dissolved, and is cooled down, and corrects pH value, high pressure steam sterilization, and heating is dissolved, and is cooled down, and is added 30% yolk salt solution, is shaken up, produce.Nutriment enriches in fine and close culture medium prepared by the present invention, the growth of strain and total plate count during Bacteria Culture can be improved, a large amount of viable bacteria thalline are provided to prepare probiotics, and by microbionation to the fine and close culture medium, its motion can be effectively observed.
Description
Technical field
The present invention relates to bacteria culture media and its preparing technical field, and in particular to a kind of fine and close training for Bacteria Culture
Support base and preparation method thereof.
Background technology
Probiotics is that one kind is colonized in human body intestinal canal, in reproductive system, can produce definite health efficacy so as to improve host
Microecological balance, the general name of the microorganism of the work to be played an important role to health, it is widely used in food, health products and medicine
In production.In general, probiotics is with significantly balance and improvement gut flora, prevention and treatment diarrhoea, raising machine
Body immunity, pre- hypo-allergenic, combination simultaneously exclude the functions such as toxin, pre- anti-caries.Research finds that probiotics is in human body intestinal canal
Quantity plays health care to probiotics and therapeutic efficiency plays decisive role.Except probiotics breeds in vivo, in process
Neutralize storage period to ensure its survival and keep outside the factors such as bioactivity, enough viable counts are Probiotic supplement product hairs
Wave the prerequisite of its validity.
The current research direction for improving probiotics viable bacteria sum be concentrated mainly on zymotechnique optimization and compound bacteria it is highly dense
Degree fermentation.Benefit materials are added into fermentation medium can not only improve the viable count of probiotics, and acidified milk can also have concurrently beneficial
The function of material.Chinese invention patent (Application No. 200910038553.5) discloses a kind of side for improving number of live bacteria of probiotics
Method, it is to add Cordceps militaris extract in conventional lactic acid bacteria fermentation medium, nourishing Northern cordyceps enrich, and can be the life of probiotics
It is long that abundant nutrition is provided, can significantly increase the viable count of Lactic Acid from Fermentation Broth bacterium, viable count of lactobacillus from 1.5 ×
108cfu/ml brings up to 1.3 × 109cfu/ml.Xu Haiyan etc. studies xylo-oligosaccharide to lactobacillus, Bifidobacterium and gut flora
The influence of growth.As a result 1% xylo-oligosaccharide is to Lactobacillus plantarum, Lactobacillus rhamnosus, bifidobacterium longum and youth bifid bar
Bacterium has obvious proliferation function.
Current proliferation of probiotics technology achieves certain achievement, and it is a kind of high that benefit materials are added into fermentation medium
The method of effect, the easy viable count for improving probiotics.Fermenting and producing cost can be not only reduced, can even be contracted in some situations
Short fermentation period.
The content of the invention
It is an object of the invention to provide a kind of fine and close culture medium for Bacteria Culture.
To realize object above, the present invention is achieved by the following technical programs：
A kind of fine and close culture medium for Bacteria Culture, the fine and close culture medium by following mass percent raw material group
Into：Tryptone 1.7-1.9%, beef extract 0.5-0.7%, yeast extract powder 0.1-0.2%, aloe polysaccharide 1.7-1.9%, sulphur
Sour magnesium 0.1-0.2%, glutamine 0.5-0.6%, 30% yolk salt solution 4.8-5.1%, agar 1.3-1.5%, potassium nitrate
0.2-0.3%, sodium dihydrogen phosphate 0.2-0.3%, cystine 1.1-1.3%, surplus are deionized water.
Preferably, the fine and close culture medium is made up of the raw material of following mass percent：Tryptone 1.85%, beef extract
0.61%th, yeast extract powder 0.12%, aloe polysaccharide 1.82%, magnesium sulfate 0.11%, glutamine 0.57%, 30% yolk salt
Water 5.00%, agar 1.40%, potassium nitrate 0.21%, sodium dihydrogen phosphate 0.25%, cystine 1.24%, surplus are deionization
Water.
Preferably, the fine and close culture medium is used for the culture of Bifidobacterium.
Preferably, the pH of the fine and close culture medium is 7.0-7.5.
Preferably, the preparation method of the 30% yolk salt solution is as follows：Egg is immersed in 3-4h in 70-80% ethanol,
Yolk is taken under sterile working, the physiological saline cooled down is added after autoclaving, is configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and is cooled down, and corrects pH value, 121 DEG C of high pressures
Steam sterilizing 10-20min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, cools down, and adds 30% yolk salt solution, shakes up, produce.
Preferably, it is cooled to 20-30 DEG C in the step S1.
Preferably, it is cooled to 45-55 DEG C in the step S2.
Beneficial effect：Nutriment enriches in fine and close culture medium prepared by the present invention, it is possible to increase during Bacteria Culture
The growth of strain and total plate count, shorten cultivation cycle, a large amount of viable bacteria thalline are provided to prepare probiotics, and by bacterium
It is inoculated on the fine and close culture medium, its motion can be effectively observed.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making
The every other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.
Embodiment 1：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.85%th, beef extract 0.61%, yeast extract powder 0.12%, aloe polysaccharide 1.82%, magnesium sulfate 0.11%, glutamine
0.57%th, 30% yolk salt solution 5.00%, agar 1.40%, potassium nitrate 0.21%, sodium dihydrogen phosphate 0.25%, cystine
1.24%, surplus is deionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 75% ethanol
3.5h, yolk is taken under sterile working, add after autoclaving the physiological saline cooled down, be configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 25 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 15min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 50 DEG C, adds 30% yolk salt solution, shakes up, produce.
Embodiment 2：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.80%th, beef extract 0.65%, yeast extract powder 0.15%, aloe polysaccharide 1.78%, magnesium sulfate 0.15%, glutamine
0.52%th, 30% yolk salt solution 4.90%, agar 1.38%, potassium nitrate 0.23%, sodium dihydrogen phosphate 0.20%, cystine
1.15%, surplus is deionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 75% ethanol
4h, yolk is taken under sterile working, add after autoclaving the physiological saline cooled down, be configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 30 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 15min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 50 DEG C, adds 30% yolk salt solution, shakes up, produce.
Obtained fine and close culture medium is used for the culture of Bifidobacterium.
Embodiment 3：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.76%th, beef extract 0.57%, yeast extract powder 0.18%, aloe polysaccharide 1.85%, magnesium sulfate 0.10%, glutamine
0.50%th, 30% yolk salt solution 5.05%, agar 1.50%, potassium nitrate 0.25%, sodium dihydrogen phosphate 0.22%, cystine
1.18%, surplus is deionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 75% ethanol
3h, yolk is taken under sterile working, add after autoclaving the physiological saline cooled down, be configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 25 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 20min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 55 DEG C, adds 30% yolk salt solution, shakes up, produce.
Obtained fine and close culture medium is used for the culture of Bifidobacterium.
Embodiment 4：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.70%th, beef extract 0.55%, yeast extract powder 0.10%, aloe polysaccharide 1.80%, magnesium sulfate 0.18%, glutamine
0.55%th, 30% yolk salt solution 4.95%, agar 1.30%, potassium nitrate 0.20%, sodium dihydrogen phosphate 0.20%, cystine
1.20%, surplus is deionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 70% ethanol
4h, yolk is taken under sterile working, add after autoclaving the physiological saline cooled down, be configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 30 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 15min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 45 DEG C, adds 30% yolk salt solution, shakes up, produce.
Embodiment 5：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.7%th, beef extract 0.7%, yeast extract powder 0.1%, aloe polysaccharide 1.9%, magnesium sulfate 0.1%, glutamine 0.6%,
30% yolk salt solution 4.8%, agar 1.5%, potassium nitrate 0.2%, sodium dihydrogen phosphate 0.3%, cystine 1.1%, surplus are to go
Ionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 3h in 80% ethanol, under sterile working
Yolk is taken, the physiological saline cooled down is added after autoclaving, is configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 20 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 10min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 45 DEG C, adds 30% yolk salt solution, shakes up, produce.
Obtained fine and close culture medium is used for the culture of Bifidobacterium.
Embodiment 6：
A kind of fine and close culture medium for Bacteria Culture, it is made up of the raw material of following mass percent：Tryptone
1.9%th, beef extract 0.5%, yeast extract powder 0.2%, aloe polysaccharide 1.7%, magnesium sulfate 0.2%, glutamine 0.5%,
30% yolk salt solution 5.1%, agar 1.3%, potassium nitrate 0.3%, sodium dihydrogen phosphate 0.2%, cystine 1.3%, surplus are to go
Ionized water.Wherein, the preparation method of 30% yolk salt solution is as follows：Egg is immersed in 4h in 70% ethanol, under sterile working
Yolk is taken, the physiological saline cooled down is added after autoclaving, is configured to 30% yolk salt solution.
It is as follows for the preparation method of the fine and close culture medium of Bacteria Culture, step：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate,
Sodium dihydrogen phosphate, cystine are added in deionized water, and heating is boiled to being completely dissolved, and are cooled to 30 DEG C, correction pH value is extremely
7.0-7.5,121 DEG C of high pressure steam sterilization 20min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 55 DEG C, adds 30% yolk salt solution, shakes up, produce.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (7)
1. a kind of fine and close culture medium for Bacteria Culture, it is characterised in that the fine and close culture medium is by following mass percent
Raw material composition：Tryptone 1.7-1.9%, beef extract 0.5-0.7%, yeast extract powder 0.1-0.2%, aloe polysaccharide 1.7-
1.9%th, magnesium sulfate 0.1-0.2%, glutamine 0.5-0.6%, 30% yolk salt solution 4.8-5.1%, agar 1.3-1.5%,
Potassium nitrate 0.2-0.3%, sodium dihydrogen phosphate 0.2-0.3%, cystine 1.1-1.3%, surplus are deionized water.
2. as claimed in claim 1 be used for Bacteria Culture fine and close culture medium, it is characterised in that the fine and close culture medium by with
The raw material composition of lower mass percent：Tryptone 1.85%, beef extract 0.61%, yeast extract powder 0.12%, aloe polysaccharide
1.82%th, magnesium sulfate 0.11%, glutamine 0.57%, 30% yolk salt solution 5.00%, agar 1.40%, potassium nitrate
0.21%th, sodium dihydrogen phosphate 0.25%, cystine 1.24%, surplus are deionized water.
3. it is used for the fine and close culture medium of Bacteria Culture as claimed in claim 1, it is characterised in that the fine and close culture medium is used for
The culture of Bifidobacterium.
4. it is used for the fine and close culture medium of Bacteria Culture as claimed in claim 1, it is characterised in that the pH of the fine and close culture medium
For 7.0-7.5.
5. it is used for the fine and close culture medium of Bacteria Culture as claimed in claim 1, it is characterised in that the 30% yolk salt solution
Preparation method is as follows：Egg is immersed in 3-4h in 70-80% ethanol, yolk is taken under sterile working, after adding autoclaving
The physiological saline of cooling, it is configured to 30% yolk salt solution.
6. the preparation method of the fine and close culture medium for Bacteria Culture as described in claim 1-5 is any, it is characterised in that step
It is rapid as follows：
S1：By tryptone, beef extract, yeast extract powder, aloe polysaccharide, magnesium sulfate, glutamine, agar, potassium nitrate, phosphoric acid
Sodium dihydrogen, cystine are added in deionized water, and heating is boiled to being completely dissolved, and is cooled down, and corrects pH value, 121 DEG C of high steams
Sterilize 10-20min, obtains agar mixture；
S2：Agar mixture is dissolved in heating, is cooled to 45-55 DEG C, adds 30% yolk salt solution, shakes up, produce.
7. it is used for the preparation method of the fine and close culture medium of Bacteria Culture as claimed in claim 6, it is characterised in that the step
20-30 DEG C is cooled in S1.
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| CN (1) | CN107384821A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998032874A1 (en) * | 1997-01-23 | 1998-07-30 | Minnesota Mining And Manufacturing Company | Growth medium for selective detection of staphylococcus |
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2017
- 2017-07-27 CN CN201710621534.XA patent/CN107384821A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998032874A1 (en) * | 1997-01-23 | 1998-07-30 | Minnesota Mining And Manufacturing Company | Growth medium for selective detection of staphylococcus |
Non-Patent Citations (3)
| Title |
|---|
| 戴必胜 等: "中草药和芦荟多糖对肉仔鸡肠道微生态、免疫功能及生产性能的影响", 《中国家禽》 * |
| 蒋林 等: "芦荟乙酰化甘露聚糖对肉仔鸡肠道主要菌群、小肠微绒毛密度、免疫功能及生产性能的影响", 《中国兽医学报》 * |
| 谢正旸 等主编: "《福建科学技术出版社》", 30 April 1994, 福建科学技术出版社 * |
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