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CN107365355B - Anti-wrinkle peptide and cosmetic comprising same - Google Patents

Anti-wrinkle peptide and cosmetic comprising same Download PDF

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Publication number
CN107365355B
CN107365355B CN201710665855.XA CN201710665855A CN107365355B CN 107365355 B CN107365355 B CN 107365355B CN 201710665855 A CN201710665855 A CN 201710665855A CN 107365355 B CN107365355 B CN 107365355B
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skin
wrinkle
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CN107365355A (en
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罗海哨
余作取
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Wenzhou Qianrui Biotechnology Co ltd
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Wenzhou Qianrui Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations

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  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Gerontology & Geriatric Medicine (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

The present invention relates to an active peptide having an anti-wrinkle effect and a cosmetic comprising the same. Specifically, the invention discloses SEQ ID NO 1 and SEQ ID NO:2, alone or in combination, for anti-wrinkle activity and a cosmetic comprising the peptides, wherein the cosmetic comprises in weight%: 1 and/or SEQ ID NO:2, 0.0005-0.001 of peptide, 0.5-2.5 of retinol, 0.5-2.5 of tremella extract, 2.0-4.0 of purslane extract, 2.0-4.0 of 1, 3-propanediol, 4.0-6.0 of polyglutamic acid, 1.5-3.5 of rosehip oil, 2.0-4.0 of soybean lecithin, 5.0-15.0 of resveratrol, 0.4-0.8 of carbomer, 2.0-4.0 of glucan, 1.0-2.0 of lactobionic acid and 1.0-2.0 of nicotinamide. The cosmetic has the effects of resisting skin wrinkles, resisting skin aging and the like.

Description

Anti-wrinkle peptide and cosmetic comprising same
Technical Field
The present invention relates to non-natural peptides with anti-wrinkle effect and their use in cosmetic and personal care compositions.
Background
Skin aging is a phenomenon of skin aging caused by natural factors or non-natural factors. After birth, the skin tissue of a person is developed increasingly, the function of the person is active gradually, and the person starts to degenerate when the person reaches a certain age, and the degeneration is gradually performed unconsciously. The growth period of skin tissues generally ends at about 25 years old, which is called as 'bending angle of skin' by some people, since then, the growth and the aging are carried out simultaneously, the elastic fibers of the skin gradually become thick, and the aging of the skin is slowly and obviously in the early aging period of 40-50 years, although the aging degree can be different from person to person.
Skin aging is the result of the combined action of the intrinsic physiological mechanism of human aging and the damage caused by external wind, sunshine, environmental pollution and the like. Reasonable nutrition can partially inhibit physiological aging, but is difficult to protect the skin from the external environment. Premature aging of the skin can only be inhibited by timely supplementation of nutrients.
While the balance of body nutrients is important to maintaining healthy skin and may not rejuvenate skin, ignoring this will greatly accelerate skin aging. Certain nutrient deficiencies, such as vitamin C, vitamin B complexes, and essential fatty acids, are known to cause various dermatitis and other skin disorders. If slightly absent (which is common but often overlooked), it may not cause clinical symptoms, but affects the ability of the skin to heal and renew.
Skin aging is usually manifested in skin elasticity, skin wrinkles and roughness, and moisturizing intensity. The simple use of moisturizers in daily life is obviously insufficient for preventing skin aging. Environmental, sunlight, pressure and other factors can cause free radicals to greatly damage skin and promote skin aging to accelerate. At this time, the skin is also prone to spots, fine lines and the like, so that an antioxidant skin care product is required. Aging causes body aging, slow skin metabolism, and degeneration of collagen and elastic fiber of skin.
French patent FR2945939 discloses a new use of a tyrosine-arginine dipeptide which restores skin elasticity, resists skin sagging, is used to prevent and/or treat skin aging, wrinkles, stretch marks, etc., improves dermal and epidermal density, restores dermal and epidermal volume.
Japanese patent application JP2011020951 discloses a preparation for improving skin laxity, which contains pelargonidin (pelargonidin) or eggplant.
International patent application WO2017022091 discloses a preparation for improving skin laxity and aging due to skin cavitation, rosemary extract and licorice extract being capable of attracting adipose stem cells, thereby causing skin laxity or aging due to cavitation of dermis.
Chinese patent No. CN105708732 discloses a composition comprising at least one selected from amines, amides and polyols as an active ingredient, which has an anti-aging effect.
Chinese invention patent CN105407866 discloses the use of a combination comprising collagen, preferably marine collagen or a hydrolysed form thereof, and vitamin B3, said use having at least one skin benefit selected from the group consisting of lightening wrinkles, promoting skin plumping or elastic condition of the skin, improving skin firmness and smoothness, improving skin hydration and improving skin radiance. The invention also relates to a combination of active agents comprising at least collagen or a hydrolysed form thereof, vitamin B3 and a tremella extract and to a related cosmetic method.
Chinese patent CN104903346 discloses a synthetic peptide, which has 20-39 amino acid residues and has the function of preventing and/or alleviating skin aging.
Chinese invention patent CN104394876 discloses a composition for preventing skin aging, which comprises tea water derived from fresh tea leaves inactivated by enzyme.
Chinese patent No. 103957998 discloses a skin anti-aging composition, in particular a skin anti-aging composition comprising myricetin or a glycoside thereof and at least one LXR α agonist skin aging refers to the appearance or manifestation of any one or more of wrinkles or sagging, poor skin barrier such as dryness, scalp itching or uneven skin tone such as age spots.
Chinese invention patent CN103655398 discloses an anti-skin aging skin cream, which is prepared from the following raw materials in parts by weight: 5-10 parts of rose hip oil, 3-7 parts of pearl powder, 25-45 parts of deionized water, 4-8 parts of ginseng extract, 3-6 parts of vaseline, 2-5 parts of liquorice, 4-7 parts of xanthan gum, 3-5 parts of glycerol, 2-5 parts of sodium hydroxide, 4-8 parts of squalane, 2-6 parts of hexadecanol, 3-5 parts of glycerin monostearate, 4-6 parts of stearic acid, 4-8 parts of ascorbic acid fatty acid ester, 1-3 parts of xanthum gum and 2-4 parts of triethanolamine. It can repair necrotic skin in deep layer, moisturize skin, brighten skin color, and remove wrinkles.
With the improvement of the living standard, people, especially women, pay more and more attention to the external appearance of the people, so that the beauty cosmetics capable of resisting the wrinkle skin are expected by people at present.
Disclosure of Invention
The present inventors have conducted intensive studies and screened peptide libraries in order to develop novel peptides having anti-wrinkle effects. Finally, the present inventors have found a novel peptide having excellent anti-wrinkle efficacy, ultimately achieving the object of the present invention.
An object of the present invention is to provide a peptide having an anti-wrinkle effect.
It is another object of the present invention to provide a composition for anti-wrinkle.
It is yet another object of the present invention to provide a method for combating wrinkles.
To achieve the object of the present invention, in a first aspect of the present invention, there is provided a peptide selected from the group consisting of:
(i) a peptide comprising or consisting of amino acid residues as follows:
Arg-Ala-Ala-Asp-Cys-Gln-Met-Pro-Cys-Leu-Ala (RAADCQMPCLA) (SEQ ID NO: 1); or
(ii) A peptide comprising or consisting of amino acid residues as follows:
Asp-Ala-Cys-Gln-His-Leu-Ala-Pro-Ser-Thr(DACQHLAPST)(SEQ ID NO:2)。
the peptide of the present invention may also be referred to as "peptide.03 peptide".
According to a preferred aspect of the present invention, there is provided a combination of peptides comprising the peptide combination of SEQ ID NO. 1 and SEQ ID NO. 2.
According to a second aspect of the present invention, there is provided an anti-wrinkle composition comprising the peptide of SEQ ID No. 1 of the present invention and a cosmetically acceptable carrier.
According to a preferred aspect of the present invention, there is provided an anti-wrinkle composition comprising the amino acid sequence of SEQ ID No. 1 and SEQ ID NO:2 and a cosmetically acceptable carrier.
According to a third aspect of the present invention, there is provided the use of a peptide of SEQ ID NO. 1 of the present invention for anti-wrinkle.
According to a preferred aspect of the present invention, there is provided SEQ ID NO 1 and SEQ ID NO:2 for use in combating wrinkles.
The present invention also provides a cosmetic for anti-wrinkle, comprising:
(a) individual peptides or combinations of peptides of the invention;
(b) an antioxidant;
(C) a humectant;
(d) a vegetable oil;
(e) lecithin;
(f) a preservative;
(g) essential oil; and
(h) and (5) purifying the water.
These and other aspects of the present invention will become apparent to those skilled in the art upon reading the following detailed description of the invention, including the exemplary embodiments and examples.
Two peptides are claimed in the present invention, namely peptide (i) having SEQ ID NO:1 or peptide (ii) having SEQ ID NO: 2. When these peptides are used in anti-wrinkle compositions, the inhibition provides an anti-wrinkle benefit.
In addition, by experimentation, it was not only demonstrated that each of these two peptides reduces sebum secretion, but also that they interact synergistically when used together.
As used herein, all terms are intended to have their ordinary meaning in the art unless otherwise specifically defined. The term "amino acid" is intended to include naturally occurring amino acids as well as non-naturally occurring amino acids, and broadly includes all small molecules having one carboxyl group and at least one primary or secondary amine group capable of forming a peptide bond. The term "peptide" is intended to include all molecules having at least one peptide bond, and thus includes dipeptides, tripeptides, oligopeptides and polypeptides having up to about 20 amino acid residues. The term "peptide" also includes structures other than amino acids having one or more linkers, spacers, or terminal groups.
The peptides of the invention are provided in a cosmetically acceptable carrier. The carrier may be hydrophobic or hydrophilic. Suitable hydrophobic carriers include, for example, waxy nonionic materials commonly used in cosmetics, such as esters and ethers of fatty alcohols and fatty acids, with carbon chain lengths of C4 to C22, preferably C8 to C18, most preferably C12 to C18.
Examples of fatty hydrophobic carriers include isopropyl myristate, isopropyl palmitate, octyl palmitate, isopropyl lanolate, acetylated lanolin alcohol, benzoate esters of C12-C15 alcohols, cetyl octanoate, cetyl palmitate, myristyl myristate, myristyl lactate, cetyl acetate, propylene glycol dicaprylate/decanoate, decyl oleate, acetylated lanolin, heptanoic stearate, diisostearate malate, octyl hydroxystearate, isopropyl isostearate, and the like.
Suitable hydrophilic carrier solutions may be, for example, glycols and alkoxylated glycols commonly used in cosmetics, including ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, and the like.
The anti-wrinkle composition may be formulated as a nutritional liquid, a serum, a cosmetic, a cream, a lotion, a serum, a spray, a stick, and other forms known to those skilled in the art. Creams and lotions are currently the preferred product forms.
In a cosmetically acceptable carrier, the concentration of the peptide may range from 1. mu.M to 200. mu.M, preferably from 10ppb to 150. mu.M, more preferably from 20. mu.M to 100. mu.M, most preferably from 36. mu.M to 50. mu.M.
Anti-wrinkle compositions typically comprise a carrier solution as described above at a level of from about 0.01% to about 90% by weight, preferably from about 0.1% to about 50% by weight, more preferably from about 0.1% to about 20% by weight, and most preferably from about 1% to about 10% by weight.
Examples of the antioxidant include butylhydroxyanisole, dibutylhydroxytoluene, sodium hydrogen sulfite, ascorbic acid and salts thereof, glutathione peroxidase, glutathione-S-transferase, catalase, superoxide dismutase, thioredoxin, taurine, thiotaurine, hypotaurine, vitamin E, and the like. Among them, ascorbic acid, glutathione, vitamin E and taurine are preferable. These antioxidants may be used in combination of 1 or more than 2, and preferably are a combination of vitamin E and taurine. When the antioxidant is added to the composition for external application to skin (for example, an anti-wrinkle nutrient solution) of the present invention, the addition ratio is not particularly limited, but is usually 0.001 to 6% by weight, preferably 0.01 to 3% by weight, and more preferably 0.2 to 2% by weight, based on the total amount of the composition for external application to skin.
Examples of the humectant include amino acids such as alanine, serine, leucine, isoleucine, threonine, glycine, proline, hydroxyproline, aspartic acid, arginine, and theanine, and derivatives thereof; polyhydric alcohols such as ethylene glycol, 1, 3-propanediol, 1, 3-butanediol, diethylene glycol, triethylene glycol, tetraethylene glycol, dipropylene glycol, di-1, 3-propanediol, and polyethylene glycol; glycol ethers such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, dipropylene glycol monoethyl ether, and dipropylene glycol monopropyl ether; sugar alcohols such as mannitol, sorbitol, erythritol, xylitol, and trehalose; phospholipids such as lecithin and hydrogenated lecithin; mucopolysaccharides such as sodium hyaluronate, sodium acetylhyaluronate, heparin analogues, sodium chondroitin sulfate, etc.; high molecular compounds such as collagen, elastin, keratin, chitin, chitosan, gelatin, polyglutamic acid, etc.; sodium lactate, sodium pyrrolidone carboxylate, etc.; chamomile extract, aloe vera extract, witch hazel extract, rosemary extract, thyme extract, tea extract, purslane extract, and other plant extracts. Among them, glycine, 1, 3-propanediol, dipropylene glycol, di-1, 3-propanediol, diethylene glycol monoethyl ether, sodium hyaluronate, sodium acetylhyaluronate, sodium pyrrolidone carboxylate, and polyglutamic acid are preferable. These humectants may be used in 1 kind or in combination of 2 or more kinds, and are preferably 1, 3-propanediol and polyglutamic acid. When the moisturizing agent is added to the composition for external application to skin (e.g., an anti-wrinkle nutrient solution) of the present invention, the proportion thereof is not particularly limited, but is usually 0.001 to 40% by weight, preferably 0.1 to 30% by weight, and more preferably 5 to 20% by weight, based on the total amount of the composition for external application to skin (e.g., an anti-wrinkle nutrient solution).
The vegetable oil is selected from rose hip oil, sweet almond oil, rose oil, jojoba oil, wheat germ oil, carrot oil, avocado oil, castor oil, avocado oil, coconut oil, palm oil, cocoa butter, palm kernel oil, soybean oil, rice bran oil, aloe oil, veronica officinalis, chenopodium album butter, shea butter, peanut oil, corn oil, macadamia nut oil, Chinese chestnut oil, seabuckthorn oil, linseed oil, passion flower oil, calendula, mango kernel oil and rose hip oil, and is preferably one or a combination of two or more of sweet almond oil, wheat germ oil and aloe oil. Preferably, the vegetable oil in the composition of the invention is wheat germ oil. When the vegetable oil is added to the composition for external application to skin (e.g., an anti-wrinkle nutrient solution) of the present invention, the amount of the vegetable oil is not particularly limited, but is usually 0.001 to 10% by weight, preferably 0.5 to 8% by weight, and more preferably 1.0 to 8.0% by weight, based on the total amount of the composition for external application to skin (e.g., an anti-wrinkle nutrient solution).
As preservative, it is selected from benzyl alcohol, phenethyl alcohol, phenoxyethanol and lauroyl arginine ethyl ester, preferably lauroyl arginine ethyl ester. When the antiseptic is added to the composition for external application to skin (e.g., an anti-wrinkle nutrient solution) of the present invention, the addition ratio is not particularly limited, but is usually 0.001 to 5% by weight, preferably 0.1 to 5% by weight, and more preferably 0.2 to 3% by weight, based on the total amount of the composition for external application to skin (e.g., an anti-wrinkle nutrient solution).
As the lecithin, it is preferably soybean lecithin. When lecithin is added to the composition for external application to skin (e.g., an anti-wrinkle nutrient solution) of the present invention, the addition ratio is not particularly limited, but is usually 0.01 to 5% by weight, preferably 0.1 to 5% by weight, and more preferably 0.2 to 2% by weight, based on the total amount of the composition for external application to skin (e.g., an anti-wrinkle nutrient solution).
Preferably, the cosmetic of the invention comprises 2.0-4.0% by weight of vitamin E, 0.5-2.5% by weight of taurine, 0.5-2.5% by weight of wheat germ oil, 2.0-4.0% by weight of purslane extract, 2.0-4.0% by weight of 1,3 propylene glycol, 4.0-6.0% by weight of polyglutamic acid, 1.5-3.5% by weight of avocado oil, 2.0-4.0% by weight of soybean lecithin, 5.0-12.0% by weight of resveratrol, 0.4-0.8% by weight of carbomer, 2.0-4.0% by weight of dextran, 1.0-2.0% by weight of lactobionic acid and 1.0-2.0% by weight of nicotinamide.
More preferably, the cosmetic of the invention comprises 0.0005 to 0.001% by weight of the amino acid sequence of SEQ ID NO:1 peptide and/or seq id NO:2 peptides. Also preferably, the cosmetic of the present invention further comprises a suitable amount of ethyl lauroyl arginate and the balance of water.
Further preferably, the cosmetic of the present invention comprises, in wt%:
SEQ ID NO:1 of peptide 0.001, retinol 0.5-2.5, tremella extract 0.5-2.5, purslane extract 2.0-4.0, 1,3 propylene glycol 2.0-4.0, polyglutamic acid 4.0-6.0, rosehip oil 1.5-3.5, soybean lecithin 2.0-4.0, resveratrol 5.0-15.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-2.0, nicotinamide 1.0-2.0 and the balance of water.
Still further, the cosmetic of the present invention comprises, in wt%:
SEQ ID NO:2, 0.001 of peptide, 0.5-2.5 of retinol, 0.5-2.5 of tremella extract, 2.0-4.0 of purslane extract, 2.0-4.0 of 1, 3-propanediol, 4.0-6.0 of polyglutamic acid, 1.5-3.5 of rosehip oil, 2.0-4.0 of soybean lecithin, 5.0-15.0 of resveratrol, 0.4-0.8 of carbomer, 2.0-4.0 of glucan, 1.0-2.0 of lactobionic acid, 1.0-2.0 of nicotinamide and the balance of water.
Still further, the cosmetic of the present invention comprises, in weight%:
SEQ ID NO:1, peptide 0.0005 of SEQ ID NO:2, 0.0005 of peptide, 0.5-2.5 of retinol, 0.5-2.5 of tremella extract, 2.0-4.0 of purslane extract, 2.0-4.0 of 1, 3-propanediol, 4.0-6.0 of polyglutamic acid, 1.5-3.5 of rosehip oil, 2.0-4.0 of soybean lecithin, 5.0-15.0 of resveratrol, 0.4-0.8 of carbomer, 2.0-4.0 of glucan, 1.0-2.0 of lactobionic acid, 1.0-2.0 of nicotinamide and the balance of water.
As used herein, "comprising" is synonymous with "including," "containing," or "characterized by," and is inclusive or open-ended and does not exclude additional unrecited elements or method steps. The term "comprising" in any of the expressions herein, particularly in describing the method, use or product of the invention, is to be understood as including those products, methods and uses which consist essentially of and consist of the recited components or elements or steps. The invention illustratively described herein suitably may be practiced in the absence of any element or elements, limitation or limitations which is not specifically disclosed herein.
The instructions contained in the anti-wrinkle product of the present invention relating to the anti-wrinkle product may contain the following: effects and efficacies (e.g. anti-wrinkles), methods of application (e.g. even application to the skin, e.g. face and neck), and possible side effects, etc.
The terms and expressions which have been employed herein are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the invention claimed. Thus, it should be understood that although the present invention has been specifically disclosed by preferred embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.
For a more clear illustration of the invention, reference is now made in detail to the following examples, which are intended to be purely exemplary of the invention and are not to be interpreted as limiting the application.
Examples
The following examples are intended to illustrate the invention in a non-limiting manner.
Example 1: effect on type I collagen Synthesis
Human skin fibroblasts were plated on 48-well plates at 1 × 105cell/ml, cultured for one day so that cell fusion reaches about 60%, and cultured on FBS-free medium for another day. DMEM medium without FBS was prepared in A, B, C, D four groups, group A with deionized water, group B with the peptide of SEQ ID NO:1 to give a concentration of 10ppm, group C with the peptide of SEQ ID NO:2 to give a concentration of 10ppm, and group D with the peptides of SEQ ID NO:1 and SEQ ID NO:2, respectively, to give a concentration of 5 ppm. Collecting cultured human skin fibroblasts, washing with phosphate buffer solution, transferring into above four groups of culture medium, and culturing in CO2The culture was carried out in an incubator for 48 hours. To evaluate the expression of Procollagen, the culture broth obtained by the above culture method was quantitatively analyzed using a type I Procollagen C-peptide Kit (Procollagen type I C-peptide (PIP) EIA Kit, Cat #. MK101, Takara, Japan). In a coated 96-well plate provided in the kit, an anti-collagen antibody as a primary antibody was treated with the supernatant at 37 ℃ for 90 minutes, then washed 3 times with TPBS (0.1% Tween 20in PBS), and after an anti-mouse IgG (mouse IgG) as a secondary antibody was reacted for 90 minutes, washed 3 times with TPBS. After an alkaline phosphatase substrate solution (1mg/mL p-nitrophenyl phosphate-diethanolamine buffer) was reacted at room temperature for 30 minutes, absorbance at a wavelength of 405nm was measured with a microplate reader.
TABLE 1
Group of Test specimen PIP(ng/ml)
A Control group 100
B Peptide SEQ ID NO 110 ppm 120
C 2 peptide 10ppm of SEQ ID NO 116
D SEQ ID NO. 1 peptide 5ppm + SEQ ID NO. 2 peptide 5ppm 141
According to the data, compared with a blank control, the two peptides can obviously increase the synthesis of the type I collagen, and the two peptides obtain an effect which is obviously better than that of a single peptide under the condition that the total protein concentration is the same, so that the two peptides have a synergistic effect on promoting the synthesis of the type I collagen.
Example 2: preparation of anti-wrinkle nutrient solution
Composition (I) Content (wt%)
The peptides of the present invention (SEQ ID NO:1) 0.001
Retinol 1.5
Tremella extract 1.5
Purslane extract 3.0
1,3 propanediol 3.0
Polyglutamic acid 5.0
Rose fruit oil 2.5
Soybean lecithin 3.0
Resveratrol 10.0
Carbomer 0.6
Glucan 3.0
Lactobionic acid 1.0
Nicotinamide 1.0
Purified water Residual amount
Total of 100
Dissolving carbomer in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into anti-wrinkle nutritional liquid.
Example 3: preparation of anti-wrinkle nutrient solution
Composition (I) Content (wt%)
The peptides of the present invention (SEQ ID NO: 2) 0.001
Retinol 0.5
Tremella extract 0.5
Purslane extract 2.0
1,3 propanediol 2.0
Polyglutamic acid 3.0
Rose fruit oil 1.5
Soybean lecithin 2.0
Resveratrol 5.0
Carbomer 0.4
Glucan 2.0
Lactobionic acid 2.0
Nicotinamide 2.0
Purified water Residual amount
Total of 100
Dissolving carbomer in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into anti-wrinkle nutritional liquid.
Example 4: preparation of anti-wrinkle nutrient solution
Figure BDA0001371759080000101
Figure BDA0001371759080000111
Dissolving carbomer in purified water, adding 1, 3-propylene glycol, stirring, adding other components, stirring, and making into anti-wrinkle nutritional liquid.
Example 5
Skin tightening efficacy test:
volunteers 30 (age 30 to 50 years)
Grouping: randomly dividing 30 persons into 3 groups of 10 persons, and administering two test articles to each person, wherein one test article is a commercial reference article, and the other test article is the anti-wrinkle nutrient solution prepared in the examples 2 to 4;
the application method comprises cleaning face in the morning and evening every day, applying appropriate amount of above wrinkle-resisting nutrient solution on the left face, and applying control containing no active ingredient on the right face.
Cycle of 28 days
The measuring instrument comprises a German CK MPA580 elasticity tester and a VISIA skin analysis tester of CANFIELD
Volunteers recorded each person's experimental data using MPA580 elasticity tester, VISIA skin analysis tester on day 28. The experimental data are as follows in table 2:
TABLE 2
Figure BDA0001371759080000121
As can be seen from the table above, the anti-wrinkle nutrient solutions of examples 2, 3 and 4 have relatively obvious anti-wrinkle effects, and the anti-wrinkle effect of the peptide of the invention on skin is proved to have certain practical effects. However, after the anti-wrinkle nutrient solution in example 4 is used for four weeks, the improvement of the skin elasticity and the wrinkle depth are better than those in examples 2 and 3, and the obvious synergistic anti-wrinkle effect is shown.
The present invention has been illustrated by the description of the preferred embodiments above, but it is to be understood that variations and modifications can be made on these embodiments by the skilled person without departing from the spirit or scope of the present invention as expressed in the following claims.
Sequence listing
<110> Winzhou Qianri Biotechnology Ltd
<120> anti-wrinkle peptide and cosmetic comprising the same
<130>2
<160>2
<170>PatentIn version 3.5
<210>1
<211>11
<212>PRT
<213> Artificial sequence
<220>
<223>sq1
<400>1
Arg Ala Ala Asp Cys Gln Met Pro Cys Leu Ala
1 5 10
<210>2
<211>10
<212>PRT
<213> Artificial sequence
<220>
<223>sq2
<400>2
Asp Ala Cys Gln His Leu Ala Pro Ser Thr
1 5 10

Claims (9)

1. A peptide, referred to as "peptide.03 peptide", consisting of SEQ ID NO: 1.
2. A combination of peptides comprising SEQ ID NO:1 and SEQ ID NO: 2.
3. A composition for anti-wrinkle comprising the peptide of claim 1, or a combination comprising the peptide of claim 2.
4. Use of the peptide of claim 1 for the preparation of a composition for anti-wrinkle.
5. Use according to claim 4, wherein the composition is in the form of a cosmetic product.
6. A cosmetic having an anti-wrinkle effect, comprising: SEQ ID NO:1 and SEQ ID NO:2, retinol, tremella extract, purslane extract, 1, 3-propanediol, polyglutamic acid, rose hip oil, soybean lecithin, resveratrol, carbomer, dextran, lactobionic acid, and nicotinamide.
7. The cosmetic according to claim 6, comprising in weight%:
SEQ ID NO:1 of peptide 0.001, retinol 0.5-2.5, tremella extract 0.5-2.5, purslane extract 2.0-4.0, 1,3 propylene glycol 2.0-4.0, polyglutamic acid 4.0-6.0, rosehip oil 1.5-3.5, soybean lecithin 2.0-4.0, resveratrol 5.0-15.0, carbomer 0.4-0.8, glucan 2.0-4.0, lactobionic acid 1.0-2.0, nicotinamide 1.0-2.0 and the balance of water.
8. The cosmetic according to claim 6, comprising in weight%:
SEQ ID NO:2, 0.001 of peptide, 0.5-2.5 of retinol, 0.5-2.5 of tremella extract, 2.0-4.0 of purslane extract, 2.0-4.0 of 1, 3-propanediol, 4.0-6.0 of polyglutamic acid, 1.5-3.5 of rosehip oil, 2.0-4.0 of soybean lecithin, 5.0-15.0 of resveratrol, 0.4-0.8 of carbomer, 2.0-4.0 of glucan, 1.0-2.0 of lactobionic acid, 1.0-2.0 of nicotinamide and the balance of water.
9. The cosmetic according to claim 6, comprising in weight%:
SEQ ID NO:1, peptide 0.0005 of SEQ ID NO:2, 0.0005 of peptide, 0.5-2.5 of retinol, 0.5-2.5 of tremella extract, 2.0-4.0 of purslane extract, 2.0-4.0 of 1, 3-propanediol, 4.0-6.0 of polyglutamic acid, 1.5-3.5 of rosehip oil, 2.0-4.0 of soybean lecithin, 5.0-15.0 of resveratrol, 0.4-0.8 of carbomer, 2.0-4.0 of glucan, 1.0-2.0 of lactobionic acid, 1.0-2.0 of nicotinamide and the balance of water.
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CN113057932A (en) * 2021-03-19 2021-07-02 李雪云 Collagen skin care product for improving skin wrinkles
CN114560912B (en) * 2022-03-28 2023-10-20 碧悦星泽(北京)生物医药科技有限公司 Polypeptide with anti-wrinkle function, compound and application thereof

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