CN107354071A - A kind of health care's Antrodia camphorata magma medicinal liquor and preparation method thereof - Google Patents
A kind of health care's Antrodia camphorata magma medicinal liquor and preparation method thereof Download PDFInfo
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- 241001486992 Taiwanofungus camphoratus Species 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 241000411851 herbal medicine Species 0.000 claims abstract description 42
- 239000000284 extract Substances 0.000 claims abstract description 40
- 241000723347 Cinnamomum Species 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000000605 extraction Methods 0.000 claims abstract description 18
- 230000000857 drug effect Effects 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 10
- 239000000470 constituent Substances 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 230000007423 decrease Effects 0.000 claims abstract description 4
- 239000002075 main ingredient Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 38
- 238000000855 fermentation Methods 0.000 claims description 28
- 230000004151 fermentation Effects 0.000 claims description 28
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 19
- 235000013339 cereals Nutrition 0.000 claims description 18
- 235000020097 white wine Nutrition 0.000 claims description 18
- 239000002609 medium Substances 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 11
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 8
- 241000005787 Cistanche Species 0.000 claims description 6
- 240000002948 Ophiopogon intermedius Species 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 244000062793 Sorghum vulgare Species 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 239000000084 colloidal system Substances 0.000 claims description 6
- 238000011049 filling Methods 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 230000003834 intracellular effect Effects 0.000 claims description 6
- 235000019713 millet Nutrition 0.000 claims description 6
- ILRLTAZWFOQHRT-UHFFFAOYSA-N potassium;sulfuric acid Chemical compound [K].OS(O)(=O)=O ILRLTAZWFOQHRT-UHFFFAOYSA-N 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 210000001082 somatic cell Anatomy 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 235000013343 vitamin Nutrition 0.000 claims description 6
- 239000011782 vitamin Substances 0.000 claims description 6
- 229940088594 vitamin Drugs 0.000 claims description 6
- 229930003231 vitamin Natural products 0.000 claims description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000003809 water extraction Methods 0.000 claims description 6
- 241001016310 Epimedium grandiflorum Species 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 5
- 235000018905 epimedium Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 240000006766 Cornus mas Species 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000013028 medium composition Substances 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000002207 metabolite Substances 0.000 abstract description 4
- 230000009466 transformation Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- 241001248610 Ophiocordyceps sinensis Species 0.000 abstract description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 241000209020 Cornus Species 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
- A61K36/296—Epimedium
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/40—Cornaceae (Dogwood family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8968—Ophiopogon (Lilyturf)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of health care's Antrodia camphorata magma medicinal liquor and preparation method thereof, including step 1 main ingredients effect constituents extraction, for obtaining the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay；Step 2 aids in drug effect constituents extraction, and for obtaining extracts of Chinese herbal medicine, extracts of Chinese herbal medicine is used to aid in the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay to give full play to drug effect；Step 3 is mixed, and by the way that the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay and extracts of Chinese herbal medicine are mixed with alcohol, ensures that the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay and extracts of Chinese herbal medicine drug effect do not decline, while be easily absorbed by the body.The present invention greatly improves biological transformation ratio by optimum culture condition, larger improves metabolite active component content, the value that cordyceps sinensis originally has is fully played, and it can prevent fermented and cultured process zymotic fluid from becoming sour rapidly, it is more effective to obtain more single-minded direction metabolites, medicinal liquor effective ingredient is high, and human body easily absorbs, is free from side effects simultaneously.
Description
【Technical field】
The present invention relates to the technical field of functional liquor, particularly a kind of health care's Antrodia camphorata magma medicinal liquor and its preparation side
The technical field of method.
【Background technology】
Chinese medicine undergoes the development of thousands of years, there is very deep understanding to the characteristic of important source material, but due in a lot
The architectural feature of herbal medicine, many effective ingredients can not be extracted utilization, and traditional way is mixed after Chinese herbal medicine is crushed；
Or solid fermentation is carried out, dry, crush, choose, be granulated, pack together with culture medium；Or liquid deep layer fermenting is carried out, by bacterium
Body filters out, then concentrated broth.First by natural resources are limited, and cause to yield poorly, and cost is high, it is difficult to popularization and application, it is full
The sufficient market demand.The more original backwardness of second solid fermentation, biological transformation ratio is low, and pollution rate is high, though certain cost can be reduced,
Because being unable to large-scale production, easily pollute and cause to be lost, be also gradually eliminated.3rd deep fermentation is due to thalline being filtered
Fall, influence some active ingredients possessed by natural material, while the utilization rate of active components of medicine is low.
【The content of the invention】
The purpose of the present invention is exactly to solve the problems of the prior art, proposes a kind of health care's Antrodia camphorata magma medicinal liquor system
Preparation Method, greatly improve biological transformation ratio by optimum culture condition, larger improves metabolite active component content,
The value that cordyceps sinensis originally has has been fully played, and can prevent fermented and cultured process zymotic fluid from becoming sour rapidly, it is more effective to obtain more
Single-minded direction metabolite.
To achieve the above object, the present invention proposes a kind of health care's Antrodia camphorata magma medicinal liquor preparation method, including such as
Lower step：
Step 1 main ingredients imitate constituents extraction, for obtaining the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay；
Step 2 aids in drug effect constituents extraction, and for obtaining extracts of Chinese herbal medicine, extracts of Chinese herbal medicine is used to aid in Cinnamomum kanahirai hay
Camphorata mycelium fermented stoste gives full play to drug effect；
Step 3 is mixed, and by the way that the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay and extracts of Chinese herbal medicine are mixed with alcohol, ensures ox
Antrodia camphorata mycelium fermented stoste and extracts of Chinese herbal medicine drug effect do not decline, while are easily absorbed by the body,
Step 4 is packed, and mixed solution is bottled in gnotobasis.
Preferably, the detailed process that the step 1 prepares the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay is as follows：
A. the culture of Antrodia camphorata slant strains
Take natural Antrodia camphorata to carry out tissue separation, cultivated on slant medium, slant strains are made；Inclined-plane culture
Used culture medium is PDA culture medium, is cultivated 8~13 days at 27 DEG C~29 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein Liquid Culture
The composition and weight of base are as follows：221~245 grams of potato, 23~25 grams of glucose, 5~7 grams of peptone, yeast extract 0.8~
1.6 grams, 0.2~0.3 gram of magnesium sulfate, 0.6~1.7 gram of potassium dihydrogen phosphate, natural pH value, the incubation time 43 at 27 DEG C~29 DEG C
~45 hours, wherein the fluid nutrient medium is in terms of 1000ml；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level of the nutrient solution for filling sterilized processing
In Submerged fermentation device, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet 36-
38g, glucose 26-32g, white sugar 3-5g, potassium dihydrogen sulfate 0.6-1.7g, magnesium sulfate 0.1-0.15g, peptone 6-8g, yeast
Medicinal extract 3-5g, amino acid 0.1-0.2g, vitamin V B1 0.005-0.015g, add water to 1000ml；
Its process is as follows：Liquid spawn, at 27 DEG C~29 DEG C, ventilating ratio 1 are accessed with 8-10% inoculum concentrations:0.5~0.7
And under conditions of culture internal pressure is 0.03-0.05MPa, continuously ferment 40-45 hours, now PH is 4.6~4.8, mycelium
Natural subsidence 100%, one-level, two level, the condition all same of three-level submerged fermentation in aforementioned process；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then it is thin for 40MPa high-pressure homogeneous crusher machine with 3T/H, processing pressure
Cell wall, suspension glue is formed, insulation placement 3~5 hours, make intracellular matter fully ooze out, obtain Cinnamomum kanahirai hay at 32~42 DEG C
Camphorata mycelium fermented stoste；
Preferably, the step 2 prepares extracts of Chinese herbal medicine
Weigh the Chinese medicine material of following weight：Tuber of dwarf lilyturf 73-79g, radix polygonati officinalis 70-74g, dogwood fruit 70-79g, saline cistanche 93-
98g, barrenwort 12-14g, above-mentioned Chinese medicine material is secondary with the water extraction of 10 times of amounts, extracts of Chinese herbal medicine is filtered to obtain, for the first time
Extraction is extracted 120 minutes at 100 DEG C of temperature, and second of extraction is extracted 60 minutes at 100 DEG C；
Preferably, the step 3：Blend after 53~56 degree of pure grains brew former base white wine and obtain finished product.
Preferably, the detailed process that the step 1 prepares the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay is as follows：
A. the culture of Antrodia camphorata slant strains
Take natural Antrodia camphorata to carry out tissue separation, cultivated on slant medium, slant strains are made；Inclined-plane culture
Used culture medium is PDA culture medium, is cultivated 11 days at 28 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein Liquid Culture
The composition and weight of base are as follows：230 grams of potato, 24 grams of glucose, 5.8 grams of peptone, 1.3 grams of yeast extract, magnesium sulfate 0.25
Gram, 0.8 gram of potassium dihydrogen phosphate, natural pH value, incubation time 44 hours at 28 DEG C, wherein the fluid nutrient medium is with 1000ml
Meter；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level of the nutrient solution for filling sterilized processing
In Submerged fermentation device, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet
36.5g, glucose 28g, white sugar 3.8g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.12g, peptone 7g, yeast extract 3.5g, ammonia
Base acid 0.13g, vitamin V B10.007g, adds water to 1000ml；
Its process is as follows：Liquid spawn, at 28 DEG C, ventilating ratio 1 are accessed with 8.6% inoculum concentration:0.6 and culture internal pressure be
Under conditions of 0.04MPa, continuously ferment 43 hours, now PH is 4.7, mycelium natural subsidence 100%, one in aforementioned process
Level, two level, the condition all same of three-level submerged fermentation；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then it is thin for 40MPa high-pressure homogeneous crusher machine with 3T/H, processing pressure
Cell wall, suspension glue is formed, insulation placement 4 hours, make intracellular matter fully ooze out, obtain Cinnamomum kanahirai hay camphorata mycelium at 35 DEG C
Fermenation raw liquid；
Preferably, the step 2 prepares extracts of Chinese herbal medicine
Weigh the Chinese medicine material of following weight：Tuber of dwarf lilyturf 75g, radix polygonati officinalis 72g, dogwood fruit 75g, saline cistanche 95g, barrenwort
13g, above-mentioned Chinese medicine material is secondary with the water extraction of 10 times of amounts, extracts of Chinese herbal medicine is filtered to obtain, extraction is in temperature for the first time
Extracted 120 minutes at 100 DEG C, second of extraction is extracted 60 minutes at 100 DEG C；
Preferably, the step 3：Blend after 55 degree of pure grains brew former base white wine and obtain finished product.
The purpose of the present invention is exactly to solve the problems of the prior art, proposes a kind of health care's Antrodia camphorata magma medicinal liquor,
Make medicinal liquor effective ingredient high, human body easily absorbs, is free from side effects simultaneously.
To achieve the above object, the present invention proposes a kind of health care's Antrodia camphorata magma medicinal liquor, including Antrodia camphorata mycelia
Body fermenation raw liquid, extracts of Chinese herbal medicine, 53~56 degree of pure grains brew former base white wine, the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, in
The proportioning that herbal medicine extract solution, 53~56 degree of pure grains brew former base white wine is 49: 3: 48.
Preferably, former base white wine is brewed including the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 55 degree of pure grains,
The proportioning that the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 55 degree of pure grains brew former base white wine is 49: 3: 48.
The feature and advantage of the present invention will be described in detail by embodiment.
【Embodiment】
A kind of health care's Antrodia camphorata magma medicinal liquor preparation method of the present invention, comprises the following steps：
Step 1 main ingredients imitate constituents extraction, for obtaining the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay；
Step 2 aids in drug effect constituents extraction, and for obtaining extracts of Chinese herbal medicine, extracts of Chinese herbal medicine is used to aid in Cinnamomum kanahirai hay
Camphorata mycelium fermented stoste gives full play to drug effect；
Step 3 is mixed, and by the way that the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay and extracts of Chinese herbal medicine are mixed with alcohol, ensures ox
Antrodia camphorata mycelium fermented stoste and extracts of Chinese herbal medicine drug effect do not decline, while are easily absorbed by the body,
Step 4 is packed, and mixed solution is bottled in gnotobasis.
The detailed process that the step 1 prepares the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay is as follows：
A. the culture of Antrodia camphorata slant strains
Take natural Antrodia camphorata to carry out tissue separation, cultivated on slant medium, slant strains are made；Inclined-plane culture
Used culture medium is PDA culture medium, is cultivated 8~13 days at 27 DEG C~29 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein Liquid Culture
The composition and weight of base are as follows：221~245 grams of potato, 23~25 grams of glucose, 5~7 grams of peptone, yeast extract 0.8~
1.6 grams, 0.2~0.3 gram of magnesium sulfate, 0.6~1.7 gram of potassium dihydrogen phosphate, natural pH value, the incubation time 43 at 27 DEG C~29 DEG C
~45 hours, wherein the fluid nutrient medium is in terms of 1000ml；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level of the nutrient solution for filling sterilized processing
In Submerged fermentation device, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet 36-
38g, glucose 26-32g, white sugar 3-5g, potassium dihydrogen sulfate 0.6-1.7g, magnesium sulfate 0.1-0.15g, peptone 6-8g, yeast
Medicinal extract 3-5g, amino acid 0.1-0.2g, vitamin V B10.005-0.015g, add water to 1000ml；
Its process is as follows：Liquid spawn, at 27 DEG C~29 DEG C, ventilating ratio 1: 0.5~0.7 are accessed with 8-10% inoculum concentrations
And under conditions of culture internal pressure is 0.03-0.05MPa, continuously ferment 40-45 hours, now PH is 4.6~4.8, mycelium
Natural subsidence 100%, one-level, two level, the condition all same of three-level submerged fermentation in aforementioned process；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then it is thin for 40MPa high-pressure homogeneous crusher machine with 3T/H, processing pressure
Cell wall, suspension glue is formed, insulation placement 3~5 hours, make intracellular matter fully ooze out, obtain Cinnamomum kanahirai hay at 32~42 DEG C
Camphorata mycelium fermented stoste；The step 2 prepares the Chinese medicine material that extracts of Chinese herbal medicine weighs following weight：Tuber of dwarf lilyturf 73-
79g, radix polygonati officinalis 70-74g, dogwood fruit 70-79g, saline cistanche 93-98g, barrenwort 12-14g, by above-mentioned Chinese medicine material with 10 times of amounts
Water extraction it is secondary, filter to obtain extracts of Chinese herbal medicine, extraction for the first time is extracted 120 minutes at 100 DEG C of temperature, is carried for the second time
Take is extracted 60 minutes at 100 DEG C；The step 3：Blend after 53~56 degree of pure grains brew former base white wine and obtain finished product.Institute
State step 1 prepare the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay detailed process it is as follows：
A. the culture of Antrodia camphorata slant strains
Take natural Antrodia camphorata to carry out tissue separation, cultivated on slant medium, slant strains are made；Inclined-plane culture
Used culture medium is PDA culture medium, is cultivated 11 days at 28 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein Liquid Culture
The composition and weight of base are as follows：230 grams of potato, 24 grams of glucose, 5.8 grams of peptone, 1.3 grams of yeast extract, magnesium sulfate 0.25
Gram, 0.8 gram of potassium dihydrogen phosphate, natural pH value, incubation time 44 hours at 28 DEG C, wherein the fluid nutrient medium is with 1000ml
Meter；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level of the nutrient solution for filling sterilized processing
In Submerged fermentation device, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet
36.5g, glucose 28g, white sugar 3.8g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.12g, peptone 7g, yeast extract 3.5g, ammonia
Base acid 0.13g, vitamin V B10.007g, adds water to 1000ml；
Its process is as follows：Liquid spawn, at 28 DEG C, ventilating ratio 1 are accessed with 8.6% inoculum concentration:0.6 and culture internal pressure be
Under conditions of 0.04MPa, continuously ferment 43 hours, now PH is 4.7, mycelium natural subsidence 100%, one in aforementioned process
Level, two level, the condition all same of three-level submerged fermentation；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then it is thin for 40MPa high-pressure homogeneous crusher machine with 3T/H, processing pressure
Cell wall, suspension glue is formed, insulation placement 4 hours, make intracellular matter fully ooze out, obtain Cinnamomum kanahirai hay camphorata mycelium at 35 DEG C
Fermenation raw liquid；The step 2 prepares the Chinese medicine material that extracts of Chinese herbal medicine weighs following weight：Tuber of dwarf lilyturf 75g, radix polygonati officinalis 72g, mountain
Cornus 75g, saline cistanche 95g, barrenwort 13g, by above-mentioned Chinese medicine material with 10 times amount water extraction it is secondary, filter Chinese herbal medicine carries
Take liquid, extraction for the first time extract 120 minutes at 100 DEG C of temperature, and second, which is extracted, is extracted 60 minutes at 100 DEG C；Institute
State step 3：Blend after 55 degree of pure grains brew former base white wine and obtain finished product.
A kind of health care's Antrodia camphorata magma medicinal liquor of the present invention, including including the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay, Chinese herbal medicine
Extract solution, 53~56 degree of pure grains brew former base white wine, the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 53~56
The proportioning for spending pure grain brew former base white wine is 49: 3: 48.Including the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 55 degree
Pure grain brews former base white wine, the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 55 degree pure grains brew former base white wine
Match as 49: 3: 48.
Above-described embodiment is the description of the invention, is not limitation of the invention, it is any to simple transformation of the present invention after
Scheme belong to protection scope of the present invention.
Claims (8)
1. a kind of health care's Antrodia camphorata magma medicinal liquor preparation method, it is characterised in that comprise the following steps：
Step 1 main ingredients imitate constituents extraction, for obtaining the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay；
Step 2 aids in drug effect constituents extraction, and for obtaining extracts of Chinese herbal medicine, extracts of Chinese herbal medicine is used to aid in Antrodia camphorata bacterium
Mycelium fermentation stoste gives full play to drug effect；
Step 3 is mixed, and by the way that the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay and extracts of Chinese herbal medicine are mixed with alcohol, ensures Antrodia camphorata
Mycelium fermentation stoste and extracts of Chinese herbal medicine drug effect do not decline, while are easily absorbed by the body,
Step 4 is packed, and mixed solution is bottled in gnotobasis.
A kind of 2. health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 1, it is characterised in that：The step
The detailed process that one, prepares the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay is as follows：
A. the culture of Antrodia camphorata slant strains
Take natural Antrodia camphorata to carry out tissue separation, cultivated on slant medium, slant strains are made；Inclined-plane culture is adopted
Culture medium is PDA culture medium, is cultivated 8~13 days at 27 DEG C~29 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein fluid nutrient medium
Composition and weight are as follows：221~245 grams of potato, 23~25 grams of glucose, 5~7 grams of peptone, 0.8~1.6 gram of yeast extract,
0.2~0.3 gram of magnesium sulfate, 0.6~1.7 gram of potassium dihydrogen phosphate, natural pH value, incubation time 43~45 is small at 27 DEG C~29 DEG C
When, wherein the fluid nutrient medium is in terms of 1000ml；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level deep layer of the nutrient solution for filling sterilized processing
In convertible culture apparatus, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet 36-38g,
Glucose 26-32g, white sugar 3-5g, potassium dihydrogen sulfate 0.6-1.7g, magnesium sulfate 0.1-0.15g, peptone 6-8g, yeast extract
3-5g, amino acid 0.1-0.2g, vitamin V B10.005-0.015g, add water to 1000ml；
Its process is as follows：Liquid spawn, at 27 DEG C~29 DEG C, ventilating ratio 1 are accessed with 8-10% inoculum concentrations:0.5~0.7 and training
Internal pressure is supported under conditions of 0.03-0.05MPa, to continuously ferment 40-45 hours, now PH is 4.6~4.8, and mycelium is naturally heavy
Drop 100%, one-level, two level, the condition all same of three-level submerged fermentation in aforementioned process；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then with 3T/H, processing pressure is 40MPa high pressure homogenizer smudge cells
Wall, suspension glue is formed, insulation placement 3~5 hours, make intracellular matter fully ooze out, obtain Antrodia camphorata at 32~42 DEG C
Mycelium fermentation stoste.
A kind of 3. health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 2, it is characterised in that：The step
Two, prepare extracts of Chinese herbal medicine
Weigh the Chinese medicine material of following weight：Tuber of dwarf lilyturf 73-79g, radix polygonati officinalis 70-74g, dogwood fruit 70-79g, saline cistanche 93-98g are excessive
Sheep leaves of pulse plants 12-14g, above-mentioned Chinese medicine material is secondary with the water extraction of 10 times of amounts, extracts of Chinese herbal medicine is filtered to obtain, extraction for the first time is
Extracted 120 minutes at 100 DEG C of temperature, second of extraction is extracted 60 minutes at 100 DEG C.
A kind of 4. health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 3, it is characterised in that：The step
Three：Blend after 53~56 degree of pure grains brew former base white wine and obtain finished product.
A kind of health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 2, it is characterised in that：The step 1
Culture medium is PDA culture medium used by middle inclined-plane culture, is cultivated 11 days at 28 DEG C；
B. the preparation of liquid spawn
Take and cultivated in the inclined-plane solid spawn liquid medium within of above-mentioned culture, liquid spawn is made；Wherein fluid nutrient medium
Composition and weight are as follows：230 grams of potato, 24 grams of glucose, 5.8 grams of peptone, 1.3 grams of yeast extract, 0.25 gram of magnesium sulfate, phosphorus
0.8 gram of acid dihydride potassium, natural pH value, incubation time 44 hours at 28 DEG C, wherein the fluid nutrient medium is in terms of 1000ml；
C. submerged fermentation expands culture
Liquid spawn is aseptically sequentially ingressed into the one-level, two level, three-level deep layer of the nutrient solution for filling sterilized processing
In convertible culture apparatus, live body bacterium solution is obtained by culture；
Wherein corresponding one-level, two level, three-level submerged fermentation culture medium all same, its composition and weight are：Millet 36.5g, Portugal
Grape sugar 28g, white sugar 3.8g, potassium dihydrogen sulfate 1.2g, magnesium sulfate 0.12g, peptone 7g, yeast extract 3.5g, amino acid
0.13g, vitamin V B10.007g, adds water to 1000ml；
Its process is as follows：Liquid spawn, at 28 DEG C, ventilating ratio 1 are accessed with 8.6% inoculum concentration:0.6 and culture internal pressure be
Under conditions of 0.04MPa, continuously ferment 43 hours, now PH is 4.7, mycelium natural subsidence 100%, one in aforementioned process
Level, two level, the condition all same of three-level submerged fermentation；
D. active somatic cells broken wall
Live body bacterium solution is ground through 3T/H colloid mills, then with 3T/H, processing pressure is 40MPa high pressure homogenizer smudge cells
Wall, suspension glue is formed, insulation placement 4 hours, make intracellular matter fully ooze out at 35 DEG C, obtain Cinnamomum kanahirai hay camphorata mycelium hair
Ferment stoste.
A kind of 5. health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 3, it is characterised in that：The step
Extracts of Chinese herbal medicine is prepared in two
Weigh the Chinese medicine material of following weight：Tuber of dwarf lilyturf 75g, radix polygonati officinalis 72g, dogwood fruit 75g, saline cistanche 95g, barrenwort 13g will be upper
It is secondary with the water extraction of 10 times of amounts to state Chinese medicine material, filters to obtain extracts of Chinese herbal medicine, extraction for the first time is carried at 100 DEG C of temperature
Take 120 minutes, second of extraction is extracted 60 minutes at 100 DEG C.
A kind of 6. health care's Antrodia camphorata magma medicinal liquor preparation method as claimed in claim 4, it is characterised in that：The step
Three：Blend after 55 degree of pure grains brew former base white wine and obtain finished product.
7. a kind of health care's Antrodia camphorata magma medicinal liquor, it is characterised in that carried including the camphorata mycelium fermented stoste of Cinnamomum kanahirai hay, Chinese herbal medicine
Take liquid, 53~56 degree of pure grains to brew former base white wine, the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, extracts of Chinese herbal medicine, 53~56 degree
The proportioning that pure grain brews former base white wine is 49：3：48.
A kind of 8. health care's Antrodia camphorata magma medicinal liquor as claimed in claim 7, it is characterised in that：Including Cinnamomum kanahirai hay camphorata mycelium
Fermenation raw liquid, extracts of Chinese herbal medicine, 55 degree of pure grains brew former base white wine, and the camphorata mycelium fermented stoste of the Cinnamomum kanahirai hay, Chinese herbal medicine carry
It is 49 to take liquid, the proportioning of 55 degree of pure grains brew former base white wine：3：48.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168968A (en) * | 2018-09-20 | 2019-01-11 | 安徽斯普瑞生物科技有限公司 | A kind of Antrodia camphorata submerged fermentation solid culture compound product |
| CN114032155A (en) * | 2021-12-10 | 2022-02-11 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata white spirit |
| CN114149882A (en) * | 2021-12-10 | 2022-03-08 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata and sea cucumber beer |
| CN114231377A (en) * | 2021-12-10 | 2022-03-25 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata and sea cucumber white spirit |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104017702A (en) * | 2014-06-24 | 2014-09-03 | 深圳市仁泰生物科技有限公司 | Preparation method of antrodia cinnamomea health wine |
| CN104232441A (en) * | 2014-09-28 | 2014-12-24 | 陈合龙 | Health caring and preserving cordyceps wine and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017702A (en) * | 2014-06-24 | 2014-09-03 | 深圳市仁泰生物科技有限公司 | Preparation method of antrodia cinnamomea health wine |
| CN104232441A (en) * | 2014-09-28 | 2014-12-24 | 陈合龙 | Health caring and preserving cordyceps wine and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168968A (en) * | 2018-09-20 | 2019-01-11 | 安徽斯普瑞生物科技有限公司 | A kind of Antrodia camphorata submerged fermentation solid culture compound product |
| CN109168968B (en) * | 2018-09-20 | 2021-03-26 | 安徽斯普瑞生物科技有限公司 | Antrodia camphorata submerged fermentation solid culture compound product |
| CN114032155A (en) * | 2021-12-10 | 2022-02-11 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata white spirit |
| CN114149882A (en) * | 2021-12-10 | 2022-03-08 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata and sea cucumber beer |
| CN114231377A (en) * | 2021-12-10 | 2022-03-25 | 大连富森智能科技有限公司 | Method for preparing antrodia camphorata and sea cucumber white spirit |
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