CN107308027A - A kind of succinct production technology of sapphire whitening - Google Patents
A kind of succinct production technology of sapphire whitening Download PDFInfo
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- CN107308027A CN107308027A CN201710563443.5A CN201710563443A CN107308027A CN 107308027 A CN107308027 A CN 107308027A CN 201710563443 A CN201710563443 A CN 201710563443A CN 107308027 A CN107308027 A CN 107308027A
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- whitening
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- 230000002087 whitening effect Effects 0.000 title claims abstract description 48
- 229910052594 sapphire Inorganic materials 0.000 title claims abstract description 43
- 239000010980 sapphire Substances 0.000 title claims abstract description 43
- 238000005516 engineering process Methods 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 26
- 241000195493 Cryptophyta Species 0.000 claims abstract description 23
- 239000002775 capsule Substances 0.000 claims abstract description 23
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 17
- 230000001954 sterilising effect Effects 0.000 claims abstract description 16
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 13
- 235000011187 glycerol Nutrition 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000003306 harvesting Methods 0.000 claims abstract description 6
- 239000003755 preservative agent Substances 0.000 claims abstract description 6
- 230000002335 preservative effect Effects 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 7
- 229960005323 phenoxyethanol Drugs 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 108010001682 Dextranase Proteins 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 abstract description 30
- 108010007013 Melanocyte-Stimulating Hormones Proteins 0.000 abstract description 10
- SFVVQRJOGUKCEG-OPQSFPLASA-N β-MSH Chemical compound C1C[C@@H](O)[C@H]2C(COC(=O)[C@@](O)([C@@H](C)O)C(C)C)=CCN21 SFVVQRJOGUKCEG-OPQSFPLASA-N 0.000 abstract description 10
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 238000003786 synthesis reaction Methods 0.000 abstract description 5
- 210000002615 epidermis Anatomy 0.000 abstract description 4
- 210000002752 melanocyte Anatomy 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 241000287828 Gallus gallus Species 0.000 abstract description 2
- 239000000796 flavoring agent Substances 0.000 abstract description 2
- 235000019634 flavors Nutrition 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 229920012196 Polyoxymethylene Copolymer Polymers 0.000 abstract 1
- 108010069820 Pro-Opiomelanocortin Proteins 0.000 abstract 1
- 102100027467 Pro-opiomelanocortin Human genes 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 17
- 102400000739 Corticotropin Human genes 0.000 description 6
- 101800000414 Corticotropin Proteins 0.000 description 6
- 101000620359 Homo sapiens Melanocyte protein PMEL Proteins 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- IDLFZVILOHSSID-OVLDLUHVSA-N corticotropin Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)NC(=O)[C@@H](N)CO)C1=CC=C(O)C=C1 IDLFZVILOHSSID-OVLDLUHVSA-N 0.000 description 6
- 229960000258 corticotropin Drugs 0.000 description 6
- 239000006071 cream Substances 0.000 description 4
- 230000008099 melanin synthesis Effects 0.000 description 4
- 230000000699 topical effect Effects 0.000 description 4
- 239000000055 Corticotropin-Releasing Hormone Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 102400000740 Melanocyte-stimulating hormone alpha Human genes 0.000 description 2
- 101710200814 Melanotropin alpha Proteins 0.000 description 2
- 101150093308 POMC gene Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 210000002780 melanosome Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- WHNFPRLDDSXQCL-UAZQEYIDSA-N α-msh Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)NC(=O)[C@H](CO)NC(C)=O)C1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UAZQEYIDSA-N 0.000 description 2
- 241001262102 Cystoseira tamariscifolia Species 0.000 description 1
- 241000195522 Fucales Species 0.000 description 1
- 101001091379 Homo sapiens Kallikrein-5 Proteins 0.000 description 1
- 101000661807 Homo sapiens Suppressor of tumorigenicity 14 protein Proteins 0.000 description 1
- 102100034868 Kallikrein-5 Human genes 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000010437 gem Substances 0.000 description 1
- 229910001751 gemstone Inorganic materials 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- WHNFPRLDDSXQCL-UHFFFAOYSA-N α-melanotropin Chemical compound C=1N=CNC=1CC(C(=O)NC(CC=1C=CC=CC=1)C(=O)NC(CCCNC(N)=N)C(=O)NC(CC=1C2=CC=CC=C2NC=1)C(=O)NCC(=O)NC(CCCCN)C(=O)N1C(CCC1)C(=O)NC(C(C)C)C(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(CCSC)NC(=O)C(CO)NC(=O)C(NC(=O)C(CO)NC(C)=O)CC1=CC=C(O)C=C1 WHNFPRLDDSXQCL-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
Landscapes
- Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of succinct production technology of sapphire whitening, this method comprises the following steps：A, harvesting and dry Europe capsule chain algae；Europe capsule chain algae extract is extracted using enzymatic method；Heating inactivates enzyme；Add glycerine and preservative；Sterilising filtration is that can obtain sapphire whitening essence；The sapphire whitening essence of the present invention is a kind of clear liquid with characteristic chicken flavor；The succinct main component is Europe capsule chain algae extract, and the extract obtained using enzymatic method in preparation process of the invention can significantly inhibit the synthesis of POMC (preceding melanotropin)；Prevent melanocyte from producing melanin；The melanin being already present in epidermis is beaten back, with extraordinary whitening function.
Description
Technical field
The present invention relates to cosmetics processing technique field, the succinct production technology of more particularly to a kind of sapphire whitening.
Background technology
Europe capsule chain algae (CYSTOSEIRA TAMARISCIFOLIA) belongs to the brown marine alga of Fucales.It is an introduces a collection
From deep-sea, such as sapphire, blue glare can be sent, colorful is in riotous profusion, the marine alga that can change colour, and we term it " ocean blue
This marine alga of jewel " is found in West Europe：From the south of UK ＆ Ireland and western bank, to Scottish the north, France and
The Hispanic Atlantic Ocean.This unique marine alga can grow into 60 centimeter lengths, when being submerged in water or meeting under wet condition
Send the brilliance of substantially green or blueness.
In terms of its whitening function mainly includes three below：
1st, the synthesis of POMC (preceding melanotropin) is significantly inhibited；
2nd, prevent melanocyte from producing melanin；
3rd, the melanin being already present in epidermis is beaten back.
In order to preferably play the whitening effect of Europe capsule chain algae, the invention provides a kind of succinct production of sapphire whitening
Technique.
The content of the invention
In order to solve the above technical problems, the present invention provides a kind of sapphire whitening succinct production technology.
A kind of succinct production technology of sapphire whitening, comprises the following steps：
A, harvesting and dry Europe capsule chain algae；
B, using enzymatic method extract Europe capsule chain algae extract；
C, heating inactivate enzyme；
D, addition glycerine and preservative；
E, sterilising filtration are that can obtain sapphire whitening essence.
It is preferred that, in described step A, in drying process, drying temperature preferably is 45-55 DEG C, and the time is that 4-6 is small
When.
It is preferred that, in described step B, using in enzymatic method extraction process, the enzyme of selection is protease, amylase and Portugal
The mixed enzyme of dextranase composition.
It is preferred that, in described step C, heating-up temperature is 70-80 DEG C, and the time is 10-20 minutes.
It is preferred that, in described step E, sterilizing is using 0.2 μm of sterilizing filter filtering.
It is preferred that, in described step E, during described sapphire whitening is succinct, the percentage by weight of each raw material is as follows：
Glycerine 48.5-50.5%
Europe capsule chain algae extract 0.8-1.5%
Phenoxyethanol 0.5-1.2%
Water 47.5-49.5%.
It is further preferred that in described step E, during described sapphire whitening is succinct, the percentage by weight of each raw material
It is as follows：
Glycerine 49.5%
Europe capsule chain algae extract 1.0%
Phenoxyethanol 1.0%
Water 48.5%.
Beneficial effects of the present invention are：The sapphire whitening essence of the present invention is a kind of limpid liquid with characteristic chicken flavor
Body；The succinct main component is Europe capsule chain algae extract, the extract obtained using enzymatic method in preparation process of the invention
The synthesis of POMC (preceding melanotropin) can be significantly inhibited；Prevent melanocyte from producing melanin；Beat back and be already present on epidermis
In melanin, with extraordinary whitening function.
Brief description of the drawings
Fig. 1：The compound experiment compares figure of melanotropin (POMC) before reduction.
Fig. 2：Reduce the maturing experiment contrast figure of corticotropin (ACTH).
Fig. 3：Reduce the compound experiment compares figure of PMEL17 genes.
Fig. 4：Reduce the compound experiment compares figure of melanin.
Embodiment
With reference to embodiment, the embodiment to the present invention is described in further detail.Following examples are used for
Illustrate the present invention, but be not limited to the scope of the present invention.
Embodiment 1
A kind of succinct production technology of sapphire whitening, comprises the following steps：
A, harvesting and dry Europe capsule chain algae；
B, using enzymatic method extract Europe capsule chain algae extract；
C, heating inactivate enzyme；
D, addition glycerine and preservative；
E, sterilising filtration are that can obtain sapphire whitening essence.
In described step A, in drying process, drying temperature preferably is 50 DEG C, and the time is 4 hours.
In described step B, using in enzymatic method extraction process, the enzyme of selection is protease, amylase and dextranase
The mixed enzyme of composition.
In described step C, heating-up temperature is 75 DEG C, and the time is 15 minutes.
In described step E, sterilizing is using 0.2 μm of sterilizing filter filtering.
In described step E, during described sapphire whitening is succinct, the percentage by weight of each raw material is as follows：
Glycerine 49.5%
Europe capsule chain algae extract 1.0%
Phenoxyethanol 1.0%
Water 48.5%.
Embodiment 2
A kind of succinct production technology of sapphire whitening, comprises the following steps：
A, harvesting and dry Europe capsule chain algae；
B, using enzymatic method extract Europe capsule chain algae extract；
C, heating inactivate enzyme；
D, addition glycerine and preservative；
E, sterilising filtration are that can obtain sapphire whitening essence.
In described step A, in drying process, drying temperature preferably is 55 DEG C, and the time is 4 hours.
In described step B, using in enzymatic method extraction process, the enzyme of selection is protease, amylase and dextranase
The mixed enzyme of composition.
In described step C, heating-up temperature is 80 DEG C, and the time is 10 minutes.
In described step E, sterilizing is using 0.2 μm of sterilizing filter filtering.
In described step E, during described sapphire whitening is succinct, the percentage by weight of each raw material is as follows：
Glycerine 50.5%
Europe capsule chain algae extract 0.8%
Phenoxyethanol 1.2%
Water 47.5%.
Embodiment 3
A kind of succinct production technology of sapphire whitening, comprises the following steps：
A, harvesting and dry Europe capsule chain algae；
B, using enzymatic method extract Europe capsule chain algae extract；
C, heating inactivate enzyme；
D, addition glycerine and preservative；
E, sterilising filtration are that can obtain sapphire whitening essence.
In described step A, in drying process, drying temperature preferably is 45 DEG C, and the time is 6 hours.
In described step B, using in enzymatic method extraction process, the enzyme of selection is protease, amylase and dextranase
The mixed enzyme of composition.
In described step C, heating-up temperature is 70 DEG C, and the time is 20 minutes.
In described step E, sterilizing is using 0.2 μm of sterilizing filter filtering.
In described step E, during described sapphire whitening is succinct, the percentage by weight of each raw material is as follows：
Glycerine 48.5%
Europe capsule chain algae extract 1.5%
Phenoxyethanol 0.5%
Water 49.5%.
The whitening effect and mechanism of the sapphire whitening essence prepared below by way of specific experiment to embodiment 1 are visited
Beg for, it is specific as follows：
Experiment 1：The compound experiment of melanotropin (POMC) before reduction
Preceding melanotropin (POMC) is a kind of gene, can promote α-MSH secretion, so as to trigger the generation of melanin.
Experimental method：Rebuild the human epidermal of black；The sapphire whitening of topical application 1% essence continuously makes in cream
With 16 days；Pass through the expression of PCR array analysis pomc genes.
As a result：As shown in Figure 1：Sapphire whitening is succinct to be reduced to the POMC influences synthesized compared to blank control group
46%, melanotropin POMC synthesis before sapphire whitening essence can be reduced, therefore, it is possible to reduce α-MSH and melanin production
It is raw.
Experiment 2：Reduce the maturing experiment of corticotropin (ACTH)
Experimental method：Rebuild the human epidermal of black；The sapphire whitening of topical application 1% essence continuously makes in cream
With 16 days；Pass through the expression of PCR array analysis pomc genes.
As a result：As shown in Figure 2：SCTE and ST14 are the genes of encoding proteins enzyme, can participate in preceding melanotropin (POMC) raw
Into corticotropin (ACTH), and activation melanin generation.
Experiment 3：Reduce the compound experiment of PMEL17 genes
The effect of PMEL17 genes：
1st, the activation of melanin：Under ultraviolet irradiation, keratinocyte can discharge preceding melanotropin --- POMC, when
When a-MSH combines its acceptor MC1r, it can activate the synthesis of melanin；2nd, the maturing of melanosome：PMEL17 genes ensure black
The maturation of ferritic；3rd, the synthesis of melanin：Pass through the activity of tyrosinase, melanosome synthesis of melanin.
Experimental method：Rebuild the human epidermal of black；The sapphire whitening of topical application 1% essence continuously makes in cream
With 16 days；Synthesize to analyze the situation of PMEL17 genes by immune labeled analysing protein.
As a result：As shown in Figure 3：The succinct influence to PMEL17 genes of sapphire whitening is reduced compared to blank control group
25%.
Experiment 4：Reduce the compound experiment of melanin
Experimental method：Rebuild the human epidermal of black；The sapphire whitening of topical application 1% essence continuously makes in cream
With 16 days；The coloring of quantitative analysis melanin.
As a result：As shown in Figure 4：The influence of the succinct coloring to melanin of sapphire whitening is compared to blank control group reduction
90%.
In summary：The succinct synthesis by significantly inhibiting POMC (preceding melanotropin) of the sapphire whitening of the present invention；It is anti-
Only melanocyte produces melanin；Beat back the melanin being already present in epidermis and reach extraordinary whitening effect.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, some improvement and modification can also be made, these improvement and modification
Also it should be regarded as protection scope of the present invention.
Claims (7)
1. the succinct production technology of a kind of sapphire whitening, it is characterised in that comprise the following steps：
A, harvesting and dry Europe capsule chain algae；
B, using enzymatic method extract Europe capsule chain algae extract；
C, heating inactivate enzyme；
D, addition glycerine and preservative；
E, sterilising filtration are that can obtain sapphire whitening essence.
2. the succinct production technology of sapphire whitening as claimed in claim 1, it is characterised in that in described step A, is dried
During, drying temperature preferably is 45-55 DEG C, and the time is 4-6 hours.
3. the succinct production technology of sapphire whitening as claimed in claim 1, it is characterised in that in described step B, is used
In enzymatic method extraction process, the enzyme of selection is the mixed enzyme of protease, amylase and dextranase composition.
4. the succinct production technology of sapphire whitening as claimed in claim 1, it is characterised in that in described step C, heating
Temperature is 70-80 DEG C, and the time is 10-20 minutes.
5. the succinct production technology of sapphire whitening as claimed in claim 1, it is characterised in that in described step E, sterilizing
Filtered using 0.2 μm of sterilizing filter.
6. the succinct production technology of sapphire whitening as claimed in claim 1, it is characterised in that described in described step E
Sapphire whitening it is succinct in, the percentage by weight of each raw material is as follows：
Glycerine 48.5-50.5%
Europe capsule chain algae extract 0.8-1.5%
Phenoxyethanol 0.5-1.2%
Water 47.5-49.5%.
7. the succinct production technology of sapphire whitening as claimed in claim 6, it is characterised in that described in described step E
Sapphire whitening it is succinct in, the percentage by weight of each raw material is as follows：
Glycerine 49.5%
Europe capsule chain algae extract 1.0%
Phenoxyethanol 1.0%
Water 48.5%.
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| FR3074045A1 (en) * | 2017-11-30 | 2019-05-31 | Societe De Courtage Et De Diffusion | COSMETIC COMPOSITION WITH ALGAE EXTRACT CYSTOSEIRA TAMARISCIFOLIA. |
| CN112933011A (en) * | 2021-03-04 | 2021-06-11 | 肌源匙(广东)皮肤管理科技有限公司 | Whitening composition and preparation process and application thereof |
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| FR3074045A1 (en) * | 2017-11-30 | 2019-05-31 | Societe De Courtage Et De Diffusion | COSMETIC COMPOSITION WITH ALGAE EXTRACT CYSTOSEIRA TAMARISCIFOLIA. |
| CN108158865A (en) * | 2018-01-12 | 2018-06-15 | 广州赛莱拉干细胞科技股份有限公司 | The reparation makeup removing breast of Europe capsule chain algae extract |
| CN108379153A (en) * | 2018-04-17 | 2018-08-10 | 广州赛莱拉干细胞科技股份有限公司 | The skin care compositions of Ji Yu Rou sprout capsule chain algae extracts and its application |
| CN112933011A (en) * | 2021-03-04 | 2021-06-11 | 肌源匙(广东)皮肤管理科技有限公司 | Whitening composition and preparation process and application thereof |
| CN112933011B (en) * | 2021-03-04 | 2021-12-28 | 肌源匙(广东)皮肤管理科技有限公司 | Whitening composition and preparation process and application thereof |
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