CN107280014A - It is a kind of to promote the compound probiotic protein powder of Instestinal motility - Google Patents
It is a kind of to promote the compound probiotic protein powder of Instestinal motility Download PDFInfo
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- CN107280014A CN107280014A CN201710652503.0A CN201710652503A CN107280014A CN 107280014 A CN107280014 A CN 107280014A CN 201710652503 A CN201710652503 A CN 201710652503A CN 107280014 A CN107280014 A CN 107280014A
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- 239000000843 powder Substances 0.000 title claims abstract description 55
- 239000006041 probiotic Substances 0.000 title claims abstract description 33
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 33
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 32
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 29
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 29
- 150000001875 compounds Chemical class 0.000 title claims abstract description 24
- 230000004899 motility Effects 0.000 title claims abstract description 22
- CAXNYFPECZCGFK-UHFFFAOYSA-N 2-phenyl-2-pyridin-2-ylacetonitrile Chemical compound C=1C=CC=NC=1C(C#N)C1=CC=CC=C1 CAXNYFPECZCGFK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 10
- 235000013373 food additive Nutrition 0.000 claims abstract description 10
- 239000002778 food additive Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 241001608472 Bifidobacterium longum Species 0.000 claims abstract description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 8
- 229940009291 bifidobacterium longum Drugs 0.000 claims abstract description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 8
- 240000007049 Juglans regia Species 0.000 claims abstract description 7
- 235000009496 Juglans regia Nutrition 0.000 claims abstract description 7
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 7
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 7
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 7
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 7
- 239000005862 Whey Substances 0.000 claims abstract description 7
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 7
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 7
- 235000011869 dried fruits Nutrition 0.000 claims abstract description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 7
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 7
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 7
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 7
- 235000019871 vegetable fat Nutrition 0.000 claims abstract description 7
- 235000020234 walnut Nutrition 0.000 claims abstract description 7
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims abstract description 6
- 229940009289 bifidobacterium lactis Drugs 0.000 claims abstract description 6
- 235000019197 fats Nutrition 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 238000004806 packaging method and process Methods 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 20
- 235000013336 milk Nutrition 0.000 claims description 14
- 239000008267 milk Substances 0.000 claims description 14
- 210000004080 milk Anatomy 0.000 claims description 14
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 238000005238 degreasing Methods 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 7
- 239000003833 bile salt Substances 0.000 claims description 7
- 230000015784 hyperosmotic salinity response Effects 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical group [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 206010034133 Pathogen resistance Diseases 0.000 claims description 5
- 210000000232 gallbladder Anatomy 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 235000019764 Soybean Meal Nutrition 0.000 claims description 4
- 210000000941 bile Anatomy 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000004455 soybean meal Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- DKKCQDROTDCQOR-UHFFFAOYSA-L Ferrous lactate Chemical compound [Fe+2].CC(O)C([O-])=O.CC(O)C([O-])=O DKKCQDROTDCQOR-UHFFFAOYSA-L 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 229960003563 calcium carbonate Drugs 0.000 claims description 3
- 229940092124 calcium citrate malate Drugs 0.000 claims description 3
- MPCMQXRREZMSPJ-UHFFFAOYSA-L calcium;2-hydroxybutanedioate;2-hydroxypropane-1,2,3-tricarboxylic acid;pentahydrate Chemical compound O.O.O.O.O.[Ca+2].[O-]C(=O)C(O)CC([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O MPCMQXRREZMSPJ-UHFFFAOYSA-L 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 239000004225 ferrous lactate Substances 0.000 claims description 3
- 235000013925 ferrous lactate Nutrition 0.000 claims description 3
- 229940037907 ferrous lactate Drugs 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000013406 prebiotics Nutrition 0.000 claims description 3
- 235000020183 skimmed milk Nutrition 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000012137 tryptone Substances 0.000 claims description 3
- 235000019155 vitamin A Nutrition 0.000 claims description 3
- 239000011719 vitamin A Substances 0.000 claims description 3
- 229940045997 vitamin a Drugs 0.000 claims description 3
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 239000011670 zinc gluconate Substances 0.000 claims description 2
- 235000011478 zinc gluconate Nutrition 0.000 claims description 2
- 229960000306 zinc gluconate Drugs 0.000 claims description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 208000024172 Cardiovascular disease Diseases 0.000 abstract description 2
- 208000026106 cerebrovascular disease Diseases 0.000 abstract description 2
- 230000001079 digestive effect Effects 0.000 abstract description 2
- 230000002526 effect on cardiovascular system Effects 0.000 abstract description 2
- 239000003053 toxin Substances 0.000 abstract description 2
- 231100000765 toxin Toxicity 0.000 abstract description 2
- 238000005303 weighing Methods 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 17
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 4
- 210000000936 intestine Anatomy 0.000 description 3
- 108010073771 Soybean Proteins Proteins 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000019710 soybean protein Nutrition 0.000 description 2
- 229960003080 taurine Drugs 0.000 description 2
- NAOLWIGVYRIGTP-UHFFFAOYSA-N 1,3,5-trihydroxyanthracene-9,10-dione Chemical compound C1=CC(O)=C2C(=O)C3=CC(O)=CC(O)=C3C(=O)C2=C1 NAOLWIGVYRIGTP-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
It is a kind of to promote the compound probiotic protein powder of Instestinal motility, it is characterised in that it is with probiotic powder（Bifidobacterium lactis, lactobacillus acidophilus, Lactobacillus casei, bifidobacterium longum, Lactobacillus plantarum）, soyabean protein powder, walnut powder, PURE WHEY, dried fruit juice, white granulated sugar, DHA algal oil, vegetable fat powder, food enrichment, food additives be primary raw material, be made through weighing dispensing, mixing, the technique such as packaging.The compound probiotic protein powder of the present invention, can not only promote Instestinal motility, effectively remove toxin and fat, increase digestive function, and protein content is high, there is certain dietotherapy effect to cardiovascular and cerebrovascular disease.
Description
Technical field
The invention belongs to protein powder field, and in particular to a kind of to promote the compound probiotic protein of Instestinal motility
Powder.
Background technology
Probiotics is that a class can improve the balance of host intestine microorganism species, active material be produced, so as to play prebiotic
The microorganism with certain activity of function, is physiology bacterium particularly important in people and animals' enteron aisle, with micro- in regulation host intestine
The ecological balance of biological flora, promotes the propagation of beneficial bacterium in enteron aisle, suppresses the growth of harmful bacteria to improve in host intestine
Bacterial community, host immunity, reduction host cholesterol level can be strengthened, the important physiological functions such as carcinogen are eliminated, it is right
It is significant in prevention diabetes, heart disease, hypertension, high fat of blood even cancer.
The content of the invention
It is an object of the invention to provide a kind of protein content it is high can promote the compound probiotic albumen of Instestinal motility
Matter powder.
In order to realize above-mentioned purpose, present invention employs following technical scheme：
It is a kind of to promote the compound probiotic protein powder of Instestinal motility, it is characterised in that it is with probiotic powder（Newborn bifid bar
Bacterium, lactobacillus acidophilus, Lactobacillus casei, bifidobacterium longum, Lactobacillus plantarum）, soyabean protein powder, walnut powder, PURE WHEY,
Dried fruit juice, white granulated sugar, DHA algal oil, vegetable fat powder, food enrichment, food additives be primary raw material, through weigh dispensing,
The techniques such as mixing, packaging are made.
Described can promote the compound probiotic protein powder of Instestinal motility, it is characterised in that it is prepared by following steps
Form：
（1）By bifidobacterium lactis kind, lactobacillus acidophilus kind, Lactobacillus casei kind, bifidobacterium longum kind, Lactobacillus plantarum kind with 1：
0.3-0.4：0.35-0.45：0.2-0.3：0.2-0.3 mass ratio is accessed in the degreasing milk medium of 400-500 times of parts by weight,
35-37 DEG C of constant temperature activation culture is preserved to degreasing milk medium curdled milk under the conditions of being placed in 0-5 DEG C；
（2）The bacterial strain of complete curdled milk is inoculated in AST culture mediums with 2-3% inoculum concentration, by reducing pH in culture medium value
Method carries out domestication of acid-resistance, and the pH value of domestication is followed successively by 7.0,6.0,5.5,5.0,4.5；The 35-37 DEG C of culture 45- under pH7.0
48h, is then seeded into pH6.0 and cultivates, and passes on 4-5 times, tames repeatedly in each pH value, and next pH is inoculated after stabilization
Value, obtains domestication of acid-resistance bacterial strain；Domestication of acid-resistance bacterial strain is inoculated in AST culture mediums with 2-3% inoculum concentration, trained by increasing
The method for supporting gallbladder salinity in base carries out bile tolerance domestication, and domestication concentration is followed successively by：0.03%、0.05%、0.075%、0.1%、
0.15%、0.2%、0.3%、0.5%；Each gallbladder salinity is placed in 35-37 DEG C of culture to stable, then activates once, finally with
2-3% inoculum concentration is inoculated into AST culture mediums, is continued the culture of next concentration, is obtained acid and bile salt tolerance bacterial strain；
（3）Acid and bile salt tolerance bacterial strain is inoculated in fermentation tank with 5-8% inoculum concentration, throughput 1：0.8, temperature control is in 35-
38 DEG C, expand culture 48-52h, bacterium number 108-1010Individual/mL, stops fermentation, thalline is collected by centrifugation, and is freeze-dried, obtains prebiotic
Bacterium powder；
（4）Select the soybean of full grains, cleaning, peeling；The soybean cleaned after peeling is added to the 70- of 6-8 times of parts by weight
In 80% ethanol water, 65-75 DEG C of extracting at constant temperature 3-5h, filtering, 40-50 DEG C of drying filter residue obtains defatted soybean meal；Will be de-
Fat dregs of beans is placed in pulverizer and crushed, and crosses 60-80 mesh sieves, obtains bean powder；Bean powder is added to the sodium hydroxide of 6-8 times of parts by weight
In the aqueous solution, 65-75 DEG C of extracting at constant temperature 2-3 times extracts 2-3h, merging filtrate every time；The pH value of filtrate is adjusted to 4.2-
4.6, stand, centrifuge, obtain protein gel；In the deionized water that protein gel is added to 5-6 times of parts by weight, body is adjusted
It is pH to neutrality, steam sterilizing, flash-evaporation dehydration, spray drying obtains soyabean protein powder
（5）By soyabean protein powder, probiotic powder, walnut powder, PURE WHEY, dried fruit juice, white granulated sugar, DHA algal oil and vegetable fat powder
With 1：0.15-0.2：0.2-0.3：0.12-0.15：0.1-0.12：0.08-0.1：0.08-0.1：0.06-0.08 mass ratio is mixed
Close, stirred with 250-300r/min rotating speed, add the food enrichment of 0.2-0.3 times of parts by weight, continue to stir
30-40min, is added the food additives of 0.03-0.04 times of parts by weight, is stirred with 500-600r/min rotating speed, is packed
.
The preparation method of the described compound probiotic protein powder that can promote Instestinal motility, it is characterised in that step（1）
Middle degreasing milk medium is that skimmed milk 10%, glucose 2%-3%, dusty yeast 0.1%-0.2%, surplus are deionized water, and 120 DEG C go out
Bacterium 7-8min, is chilled to room temperature.
The preparation method of the described compound probiotic protein powder that can promote Instestinal motility, it is characterised in that step（2）
In AST culture mediums be sucrose 1%, it is tryptone 0.8%-1%, dusty yeast 0.4%-0.5%, potassium dihydrogen phosphate 0.2%-0.3%, remaining
Measure as deionized water, 120 DEG C of sterilizing 12-15min naturally cool to room temperature.
The preparation method of the described compound probiotic protein powder that can promote Instestinal motility, it is characterised in that step（4）
Middle sodium hydrate aqueous solution is 4-5% sodium hydrate aqueous solution.
The preparation method of the described compound probiotic protein powder that can promote Instestinal motility, it is characterised in that step（5）
Middle food enrichment is calcium carbonate, calcium citrate malate, ferrous lactate, zinc gluconate, taurine, vitamin A, dimension
Raw element D3 is with 1：0.04-0.06：0.03-0.04：0.03-0.04：0.02-0.03：0.015-0.02：0.012-0.015 matter
Amount is formed than being mixed with.
The preparation method of the described compound probiotic protein powder that can promote Instestinal motility, it is characterised in that step（5）
Middle food additives are citric acid.
By above-mentioned technical scheme, the beneficial effects of the invention are as follows：
The compound probiotic protein powder that the present invention is provided, it is with probiotic powder（Bifidobacterium lactis, lactobacillus acidophilus, cheese breast
Bacillus, bifidobacterium longum, Lactobacillus plantarum）, soyabean protein powder, walnut powder, PURE WHEY, dried fruit juice, white granulated sugar, DHA algal
Oil, vegetable fat powder, food enrichment, food additives are primary raw material, are made through weighing the techniques such as dispensing, mixing, packaging；
It, which is rich in soybean protein, soybean protein, contains eight kinds of amino acid necessary to human body, without cholesterol, to cardiovascular and cerebrovascular disease
There is certain dietotherapy effect, there is protect liver, improve blood vessel, anti-aging and reducing blood lipid；The present invention is with bifidobacterium lactis, thermophilic
Lactobacillus lactis, Lactobacillus casei, bifidobacterium longum, Lactobacillus plantarum prepare probiotic powder, and bacterium is tamed by acidproof and bile tolerance
Strain, selects the compound strain of acid and bile salt tolerance, vigor is still powerful after being taken in by human body, can promote Instestinal motility, effectively removes
Toxin and fat, increase digestive function；The compound probiotic protein powder of the present invention, scientific and reasonable, the eating mouth feel of formula collocation
Good, health-care effect is good.
Embodiment
The present embodiment can promote the compound probiotic protein powder of Instestinal motility, and it is prepared from by following steps：
（1）By bifidobacterium lactis kind, lactobacillus acidophilus kind, Lactobacillus casei kind, bifidobacterium longum kind, Lactobacillus plantarum kind with 1：
0.4：0.35：0.2：0.3 mass ratio is accessed in the degreasing milk medium of 500 times of parts by weight, and 37 DEG C of constant temperature activation cultures are to de-
Fat breast culture medium curdled milk, is preserved under the conditions of being placed in 5 DEG C；
（2）The bacterial strain of complete curdled milk is inoculated in AST culture mediums with 3% inoculum concentration, by the side for reducing pH in culture medium value
Method carries out domestication of acid-resistance, and the pH value of domestication is followed successively by 7.0,6.0,5.5,5.0,4.5；37 DEG C of culture 48h under pH7.0, then
It is inoculated into pH6.0 and cultivates, pass on 5 times, tame repeatedly in each pH value, inoculates next pH value after stabilization, obtain resistance to
Sour naturalized strain；Domestication of acid-resistance bacterial strain is inoculated in AST culture mediums with 3% inoculum concentration, it is dense by increasing cholate in culture medium
The method of degree carries out bile tolerance domestication, and domestication concentration is followed successively by：0.03%、0.05%、0.075%、0.1%、0.15%、0.2%、
0.3%、0.5%；Each gallbladder salinity is placed in 37 DEG C of cultures to stable, then activates once, is finally inoculated with 3% inoculum concentration
Into AST culture mediums, continue the culture of next concentration, obtain acid and bile salt tolerance bacterial strain；
（3）Acid and bile salt tolerance bacterial strain is inoculated in fermentation tank with 5% inoculum concentration, throughput 1：0.8, temperature control at 35 DEG C,
Expand culture 48h, bacterium number 1010Individual/mL, stops fermentation, thalline is collected by centrifugation, and is freeze-dried, obtains probiotic powder；
（4）Select the soybean of full grains, cleaning, peeling；Soybean after cleaning is removed the peel is added to the 80% of 8 times of parts by weight
In ethanol water, 75 DEG C of extracting at constant temperature 5h, filtering, 50 DEG C of drying filter residues obtain defatted soybean meal；Defatted soybean meal is placed in crushing
Crushed in machine, cross 80 mesh sieves, obtain bean powder；In the sodium hydrate aqueous solution that bean powder is added to 8 times of parts by weight, 75 DEG C of constant temperature leachings
Carry 3 times, 3h, merging filtrate are extracted every time；The pH value of filtrate is adjusted to 4.5, stood, centrifuges, obtains protein gel；Will
Protein gel is added in the deionized water of 6 times of parts by weight, regulation system pH to neutrality, steam sterilizing, flash-evaporation dehydration, and spraying is dry
It is dry, obtain soyabean protein powder
（5）By soyabean protein powder, probiotic powder, walnut powder, PURE WHEY, dried fruit juice, white granulated sugar, DHA algal oil and vegetable fat powder
With 1：0.2：0.3：0.15：0.12：0.1：0.1：0.08 mass ratio mixing, is stirred with 300r/min rotating speed, added
The food enrichment of 0.3 times of parts by weight, continues to stir 40min, adds the food additives of 0.04 times of parts by weight, with
600r/min rotating speed stirs, packaging.
The step of the present embodiment（1）Middle degreasing milk medium is that skimmed milk 10%, glucose 3%, dusty yeast 0.2%, surplus are
Deionized water, 120 DEG C of sterilizing 8min, is chilled to room temperature.
The step of the present embodiment（2）In AST culture mediums be sucrose 1%, tryptone 1%, dusty yeast 0.5%, biphosphate
Potassium 0.3%, surplus are deionized water, and 120 DEG C of sterilizing 15min naturally cool to room temperature.
The step of the present embodiment（4）Middle sodium hydrate aqueous solution is 5% sodium hydrate aqueous solution.
The step of the present embodiment（5）Middle food enrichment is calcium carbonate, calcium citrate malate, ferrous lactate, Portugal
Grape saccharic acid zinc, taurine, vitamin A, vitamine D3 are with 1：0.06：0.04：0.04：0.03：0.015：0.012 mass ratio is mixed
Conjunction is prepared from.
The step of the present embodiment（5）Middle food additives are citric acid.
Claims (7)
1. a kind of can promote the compound probiotic protein powder of Instestinal motility, it is characterised in that it is with probiotic powder（Newborn bifid bar
Bacterium, lactobacillus acidophilus, Lactobacillus casei, bifidobacterium longum, Lactobacillus plantarum）, soyabean protein powder, walnut powder, PURE WHEY,
Dried fruit juice, white granulated sugar, DHA algal oil, vegetable fat powder, food enrichment, food additives be primary raw material, through weigh dispensing,
The techniques such as mixing, packaging are made.
2. the compound probiotic protein powder of Instestinal motility can be promoted according to claims 1, it is characterised in that its by
Following steps are prepared from：
（1）By bifidobacterium lactis kind, lactobacillus acidophilus kind, Lactobacillus casei kind, bifidobacterium longum kind, Lactobacillus plantarum kind with 1：
0.3-0.4：0.35-0.45：0.2-0.3：0.2-0.3 mass ratio is accessed in the degreasing milk medium of 400-500 times of parts by weight,
35-37 DEG C of constant temperature activation culture is preserved to degreasing milk medium curdled milk under the conditions of being placed in 0-5 DEG C；
（2）The bacterial strain of complete curdled milk is inoculated in AST culture mediums with 2-3% inoculum concentration, by reducing pH in culture medium value
Method carries out domestication of acid-resistance, and the pH value of domestication is followed successively by 7.0,6.0,5.5,5.0,4.5；The 35-37 DEG C of culture 45- under pH7.0
48h, is then seeded into pH6.0 and cultivates, and passes on 4-5 times, tames repeatedly in each pH value, and next pH is inoculated after stabilization
Value, obtains domestication of acid-resistance bacterial strain；Domestication of acid-resistance bacterial strain is inoculated in AST culture mediums with 2-3% inoculum concentration, trained by increasing
The method for supporting gallbladder salinity in base carries out bile tolerance domestication, and domestication concentration is followed successively by：0.03%、0.05%、0.075%、0.1%、
0.15%、0.2%、0.3%、0.5%；Each gallbladder salinity is placed in 35-37 DEG C of culture to stable, then activates once, finally with
2-3% inoculum concentration is inoculated into AST culture mediums, is continued the culture of next concentration, is obtained acid and bile salt tolerance bacterial strain；
（3）Acid and bile salt tolerance bacterial strain is inoculated in fermentation tank with 5-8% inoculum concentration, throughput 1：0.8, temperature control is in 35-
38 DEG C, expand culture 48-52h, bacterium number 108-1010Individual/mL, stops fermentation, thalline is collected by centrifugation, and is freeze-dried, obtains prebiotic
Bacterium powder；
（4）Select the soybean of full grains, cleaning, peeling；The soybean cleaned after peeling is added to the 70- of 6-8 times of parts by weight
In 80% ethanol water, 65-75 DEG C of extracting at constant temperature 3-5h, filtering, 40-50 DEG C of drying filter residue obtains defatted soybean meal；Will be de-
Fat dregs of beans is placed in pulverizer and crushed, and crosses 60-80 mesh sieves, obtains bean powder；Bean powder is added to the sodium hydroxide of 6-8 times of parts by weight
In the aqueous solution, 65-75 DEG C of extracting at constant temperature 2-3 times extracts 2-3h, merging filtrate every time；The pH value of filtrate is adjusted to 4.2-
4.6, stand, centrifuge, obtain protein gel；In the deionized water that protein gel is added to 5-6 times of parts by weight, body is adjusted
It is pH to neutrality, steam sterilizing, flash-evaporation dehydration, spray drying obtains soyabean protein powder
（5）By soyabean protein powder, probiotic powder, walnut powder, PURE WHEY, dried fruit juice, white granulated sugar, DHA algal oil and vegetable fat powder
With 1：0.15-0.2：0.2-0.3：0.12-0.15：0.1-0.12：0.08-0.1：0.08-0.1：0.06-0.08 mass ratio is mixed
Close, stirred with 250-300r/min rotating speed, add the food enrichment of 0.2-0.3 times of parts by weight, continue to stir
30-40min, is added the food additives of 0.03-0.04 times of parts by weight, is stirred with 500-600r/min rotating speed, is packed
.
3. the preparation method of the compound probiotic protein powder that can promote Instestinal motility according to claims 2, it is special
Levy and be, step（1）Middle degreasing milk medium is skimmed milk 10%, glucose 2%-3%, dusty yeast 0.1%-0.2%, surplus to go
Ionized water, 120 DEG C of sterilizing 7-8min, is chilled to room temperature.
4. the preparation method of the compound probiotic protein powder that can promote Instestinal motility according to claims 2, it is special
Levy and be, step（2）In AST culture mediums be sucrose 1%, tryptone 0.8%-1%, dusty yeast 0.4%-0.5%, biphosphate
Potassium 0.2%-0.3%, surplus are deionized water, and 120 DEG C of sterilizing 12-15min naturally cool to room temperature.
5. the preparation method of the compound probiotic protein powder that can promote Instestinal motility according to claims 2, it is special
Levy and be, step（4）Middle sodium hydrate aqueous solution is 4-5% sodium hydrate aqueous solution.
6. the preparation method of the compound probiotic protein powder that can promote Instestinal motility according to claims 2, it is special
Levy and be, step（5）Middle food enrichment is calcium carbonate, calcium citrate malate, ferrous lactate, zinc gluconate, ox
Sulfonic acid, vitamin A, vitamine D3 are with 1：0.04-0.06：0.03-0.04：0.03-0.04：0.02-0.03：0.015-0.02：
0.012-0.015 mass ratio, which is mixed with, to be formed.
7. the preparation method of the compound probiotic protein powder that can promote Instestinal motility according to claims 2, it is special
Levy and be, step（5）Middle food additives are citric acid.
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| CN107912589A (en) * | 2017-12-21 | 2018-04-17 | 广州莱可福生物科技有限公司 | Containing probiotics and enteron aisle and the pressed candy of immunity and preparation method thereof can be improved |
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| CN113207998A (en) * | 2021-05-19 | 2021-08-06 | 南京泛成生物科技有限公司 | Preparation method and application of vegetable protein powder with low FODMAPs (FODMAPs) |
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Application publication date: 20171024 |