CN107271690A - A kind of proteantigen detecting system and detection method of quality control - Google Patents
A kind of proteantigen detecting system and detection method of quality control Download PDFInfo
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- CN107271690A CN107271690A CN201710642994.0A CN201710642994A CN107271690A CN 107271690 A CN107271690 A CN 107271690A CN 201710642994 A CN201710642994 A CN 201710642994A CN 107271690 A CN107271690 A CN 107271690A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
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Abstract
The present invention proposes a kind of proteantigen detecting system and detection method of quality control, the proteantigen in thing is examined to detect target by a proteantigen probe, so that detection time is shorter, accuracy of detection is higher, it is simple to operate, and it is easy to those skilled in the art to analyze the testing result of storage, reduce testing cost；Further, quality control is carried out to the reaction member for being coated with proteantigen probe in above-mentioned proteantigen detecting system, qualified reaction member can be filtered out to detect proteantigen, the accuracy of proteantigen detection is improved, further, by being coated with reaction member in advance, and its quality is controlled, the time of detection proteantigen is shortened, the sensitivity of detection is improved, the professional technique requirement also corresponding reduction to operating personnel.
Description
Technical field
The present invention relates to bioscience research field, more particularly to a kind of system for detecting proteantigen and detection quality
Control method.
Background technology
Existing proteantigen detection is typically to be used to purchase in laboratory environment by the operating personnel Jing Guo professional training
Buy or homemade kit and combine such as fluorescence microscope instrument and complete.There is covered before dispatching from the factory to be measured in kit
The corresponding antibody of target protein antigen in sample.Testing sample usually requires to pre-process by centrifugation etc. in laboratory, this
Outside, during part detection also needs to buy the secondary antibody crossed of fluorescence labeling to complete, and test experience result is typically observation kit
The change of color, or obtained with fluorescence microscopy fluorescent brightness, achieved if necessary to by hand can also record result.
Based on this, the present invention proposes a kind of system for detecting proteantigen and detection method of quality control, operation letter
Single, accuracy of detection is higher, and has carried out quality control to the reaction member in the system of the detection proteantigen, and screening is closed
The reaction member for being coated with proteantigen probe of lattice, improves the accuracy of detection.
The content of the invention
It is an object of the invention to provide the system of detection proteantigen and detection method of quality control so that during detection
Between it is shorter, accuracy of detection is higher, and simple to operate, requires relatively low to the professional technique of operating personnel.
In order to achieve the above object, one aspect of the present invention provides a kind of proteantigen detecting system, for detecting mesh
Mark examines the proteantigen in thing, it is characterised in that including reaction member, measuring unit and memory cell；The reaction
Unit is coated with a proteantigen probe, and the target for being opposite in the reaction member examines the proteantigen in thing
Detected, the measuring unit is used to measuring proteantigen data message in the reaction member, and stored to
The memory cell.
Optionally, in above-mentioned proteantigen detecting system, the reaction member includes a reaction chamber, the protein
Antigen probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in above-mentioned proteantigen detecting system, the proteantigen probe includes and the protein
The corresponding protein antibody of antigen.
Optionally, in above-mentioned proteantigen detecting system, also include at least on the detecting electrode of the reaction member
One layer of closure.
In order to achieve the above object, another aspect of the invention provides a kind of to the progress quality control of any of the above-described reaction member
The method of system, it is characterised in that including：
On the detecting electrode that proteantigen probe is coated in a reaction member to be detected；
First liquid is placed in the reaction member, first liquid include deionized water, ultra-pure water, PBS solution,
Any of PBS-T solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and obtained relative to the phase place change of pumping signal, and with this
The data message of reaction member to be detected, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductance point
One in amount and capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, the data letter of the qualified reaction member is contrasted
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, in addition to：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, weight
The reaction member newly is coated with using the proteantigen probe, the proteantigen probe includes anti-with the protein
Former corresponding protein antibody；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and obtained relative to the phase place change of pumping signal, and with this
The data message of reaction member to be detected, the data message includes impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, the data letter of the qualified reaction member is contrasted
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, the proteantigen probe coating institute is reused
The step of stating reaction member includes：
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Each reaction member does the scanning of frequency electrical conductivity, and recording responses electric current and phase difference simultaneously select several values in scanning result,
It is used as the characteristic parameter CS of the reaction member to be detected0；
The first liquid is poured out, the proteantigen probe solution of the first concentration is placed in inside reaction member to be detected, pressed
At 10-40 degrees Celsius, relative humidity is places in 70-90 space, the proteantigen probe solution includes within 0-120 hours
Protein antibody solution corresponding with the proteantigen；First concentration is relative with the concentration of the target detection thing
Should；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, and recording responses electric current and phase difference simultaneously select several values in scanning result, are used as this
The characteristic parameter CS of reaction member1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, by 0-120 hours in 20-30 degrees Celsius, phase
It is placement in 70-100 space to humidity；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, and recording responses electric current and phase difference simultaneously select several values in scanning result, are used as this
The characteristic parameter CS of reaction member2；And
The first liquid is poured out, examines thing to be put into the target of known the first concentration for including proteantigen to be detected anti-
Answer in unit, perform inspection, three coating parameter CS are obtained respectively0、CS1、CS2It is used as the reaction member under the first concentration
Parameter is coated with, each parameter corresponding standing time, temperature and humidity is obtained.
Optionally, in above-mentioned reaction member method of quality control, the first liquid is poured out, the protein of the first concentration is resisted
Former probe solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is 70-90
Space in place the step of after also include：
Swashing for 10Hz~10MHz is applied to the reaction member to be detected of the proteantigen probe solution containing the first concentration
Encourage signal.
Optionally, in above-mentioned reaction member method of quality control, the proteantigen probe coating institute is reused
The step of stating reaction member to be detected also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
In summary, the present invention proposes a kind of proteantigen detecting system, by being coated with advance in the reaction cell
One proteantigen probe is to examine the proteantigen in thing to detect target so that detection time is shorter, and detection is smart
Degree is higher, and the professional technique of operating personnel is required to reduce, simple to operate；Further, target inspection thing is placed in described anti-
Answer in unit, it is increased with the proteantigen probe contact area, add detection accuracy；Surveyed by measuring unit
The proteantigen data message in the reaction member is measured, and data message is stored to memory cell, testing result
It is easy to achieve, and is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
Brief description of the drawings
Fig. 1 is the proteantigen detecting system structural representation in one embodiment of the present invention；
Fig. 2 is the flow chart for the method that quality control is carried out to the reaction member of embodiment in Fig. 1；
Fig. 3 re-starts the method stream of coating protein matter antigen probe for the reaction member of embodiment in Fig. 2 when unqualified
Cheng Tu.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- reaction members.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that, accompanying drawing uses very simplified form and equal
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of proteantigen detecting system, for detecting that target examines the proteantigen in thing, ginseng
Examine Fig. 1, including reaction member 3, measuring unit 1 and memory cell 2；The reaction member 3 is coated with proteantigen spy
Pin, the target for being opposite in the reaction member 3 examines the proteantigen in thing to be detected, the measuring unit 1
For measuring the proteantigen data message in the reaction member 3, and stored to the memory cell 2.
It is preferred that, the reaction member 3 in the present embodiment includes a reaction chamber, and the proteantigen probe is coated in described
On one detecting electrode of the bottom of reaction chamber, the detecting electrode is connected with the measuring unit 1.In detection process, target inspection
Test thing to react in the reaction chamber of reaction member 3 with the proteantigen probe, the reaction of 1 pair of measuring unit is obtained
The proteantigen data message obtained is measured, and is stored into the memory cell 2.
Optionally, the data message of the proteantigen in the present invention includes but is not limited to the impedance of the reaction member
The impedance value of the reaction member is transmitted to a memory cell 2 and stored by value changes, detection module 12.Proteantigen with
Antibody antigen reaction occurs for protein antibody, can change the thickness of the detecting electrode of the reaction member, therefore, reaction member
Impedance value can change, it is preferred that in one embodiment of the invention, the change in impedance value of reaction member 3 be detected, by the reaction
The impedance value of unit 3 is transmitted to a memory cell 2 and stored.
Optionally, the change in impedance value for the reaction member 3 that those skilled in the art can be in the present embodiment, analysis is obtained
Testing result, the testing result includes but is not limited to examine proteantigen species, the quantity and just in thing for the target
Reason condition etc.；Optionally, can also according to the antibody antigen response analysis composition of proteantigen etc., the present invention to this
Any limitation is not done.
Specifically, the reaction member is coated with proteantigen probe, and the proteantigen probe can with it is described
Target examines the proteantigen in thing to occur antibody antigen reaction, and the proteantigen probe includes but is not limited to be albumen
Matter antibody, the present invention does not do any limitation to this.
Optionally, at least one layer of closure is also included on the detecting electrode of the reaction member 3.The closure is typically adopted
Prepared with unrelated protein, prevent other materials are adsorbed onto on detecting electrode from being impacted to testing result, to improve the standard of detection
True property.
, can be with specifically, with reference to Fig. 2, quality control is carried out to the reaction member in above-mentioned proteantigen detecting system
Filter out qualified reaction member to detect proteantigen, improve the accuracy of proteantigen detection, further, lead to
Advance coating protein matter antigen probe is crossed, and its quality is controlled, the time of detection proteantigen is shortened, to operation
The professional technique requirement of personnel also corresponding reduction.
Specifically, the method for carrying out quality control to the reaction member in above-mentioned proteantigen detecting system includes：
Step S1：On the detecting electrode that proteantigen probe is coated in a reaction member to be detected；
Step S2：First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS
Any of solution, PBS-T solution and Buffer B；
Step S3：One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, is treated described
Detect that the other end of reaction member measures the size of current of response signal, and relative to the phase place change of pumping signal, and with
This obtains the data message of reaction member to be detected；
Optionally, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductive component and electric capacity
One in component or any combination；The present invention does not do any limitation to this；
Step S4：The data message of a qualified reaction member is obtained by step S1-S3, the qualified reaction member is contrasted
Data message it is whether consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected
It is qualified.
Optionally, in one embodiment of the present invention, when the qualified reaction member data message with it is described to be detected
When the data message of reaction member is inconsistent, reuses proteantigen probe solution and be coated with the reaction member, the egg
White matter antigen probe solution includes protein antibody solution corresponding with the proteantigen, and the present invention does not make any limit to this
System；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and obtained relative to the phase place change of pumping signal, and with this
The data message of reaction member to be detected, the data message includes impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；
The data message of a qualified reaction member is obtained using above-mentioned steps, the data letter of the qualified reaction member is contrasted
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in one embodiment of the present invention, with reference to Fig. 3, the proteantigen probe coating institute is reused
Reaction member to be detected is stated to comprise the following steps.
Step Sa：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and several for selecting scanning result to each reaction member
Value, is used as the characteristic parameter CS of the reaction member to be detected0；
Step Sb：The first liquid is poured out, the proteantigen probe solution of the first concentration is placed in reaction member to be detected
Inside, by 0-120 hours at 20-30 degrees Celsius, relative humidity is places in 70-90 space, the proteantigen probe
Solution includes the solution of protein antibody corresponding with the proteantigen；Optionally, it 0 hour is the first concentration to place
The moment that proteantigen probe solution is placed in inside reaction member to be detected.First concentration and the target detection thing
Concentration is corresponding.
It is preferred that, it is molten that the proteantigen probe solution includes protein antibody corresponding with the proteantigen
Liquid；The protein antibody can examine the proteantigen in thing to react with the target, can change the reaction member 3
Impedance value, in one embodiment of the invention, the change by the impedance value of the reaction member to be detected is anti-to the protein
Original is detected；Optionally, can according to the antibody antigen response analysis composition and concentration of proteantigen etc., this hair
It is bright not do any limitation to this.
Step Sc：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member to be detected, was taken the photograph by 0-120 hours in 20-30
Family name's degree, relative humidity is placement in 70-100 space；
Step Se：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected2；
Step Sf：The first liquid is poured out, examines thing to be put into the target of known the first concentration for including proteantigen
In reaction member to be detected, inspection is performed, three coating parameter CS are obtained respectively0、CS1、CS2It is used as reaction member under the concentration
Coating parameter, obtain each parameter corresponding standing time, temperature and humidity.
Optionally, step Sg is also included after Sb steps：To treating for the proteantigen probe solution containing the first concentration
Detect that reaction member applies 10Hz~10MHz pumping signal.Be easy to be internally formed significant thermograde in reaction member and
Caused local fluid flow, coating speed of the increase proteantigen probe solution on detecting electrode, the present invention to this not
Do any limitation.
Optionally, in one embodiment of the invention, reuse the proteantigen probe and be coated with the reaction to be detected
The step of unit, also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Reaction member is cleaned by cleaning liquid, other particulate matters or ion in filtering reaction member can increase
Plus coating precision, so as to improve the accuracy of detection of reaction member.
The target for being applied to the first concentration by filtering out examines the reaction member of thing, and the target to first concentration is examined
The proteantigen tested in thing is detected, on the one hand in advance to reaction member coating protein matter antigen probe, reduces detection
Time, on the other hand, the qualified reaction member selected by step Sa- steps Sf can obtain corresponding standing time, temperature
And humidity, increase the accuracy of detection, meanwhile, testing process is simple, and the professional technique to operating personnel requires to reduce, so that
Testing cost is reduced, with feasibility.
In summary, the present invention proposes a kind of proteantigen detecting system, by being coated with advance in the reaction cell
One proteantigen probe is to examine the proteantigen in thing to detect target so that detection time is shorter, and detection is smart
Degree is higher, and the professional technique of operating personnel is required to reduce, simple to operate；Further, target inspection thing is placed in described anti-
Answer in unit, it is increased with the proteantigen probe contact area, add detection accuracy；Surveyed by measuring unit
The proteantigen data message in the reaction member is measured, and data message is stored to memory cell, testing result
It is easy to achieve, and is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
The preferred embodiments of the present invention are above are only, any restriction effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
1. a kind of proteantigen detecting system, for detecting that target examines the proteantigen in thing, it is characterised in that including
Reaction member, measuring unit and memory cell；The reaction member is coated with a proteantigen probe, for being opposite to
Stating the target in reaction member examines the proteantigen in thing to be detected, the measuring unit is used to measure the reaction list
Proteantigen data message in member, and stored to the memory cell.
2. proteantigen detecting system as claimed in claim 1, it is characterised in that the reaction member includes a reaction
Chamber, the proteantigen probe is coated on a detecting electrode of the bottom of the reaction chamber, the detecting electrode with it is described
Measuring unit is connected.
3. proteantigen detecting system as claimed in claim 2, it is characterised in that the proteantigen probe include with
The corresponding protein antibody of the proteantigen.
4. proteantigen detecting system as claimed in claim 2, it is characterised in that on the detecting electrode of the reaction member
Also include at least one layer of closure.
5. a kind of method that any reaction member to as described in claim 1-4 carries out quality control, it is characterised in that bag
Include：
On the detecting electrode that proteantigen probe is coated in a reaction member to be detected；
First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS solution, PBS-T
Any of solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected
The other end of member measures the size of current of response signal, and obtains to be checked relative to the phase place change of pumping signal, and with this
Survey reaction member data message, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductive component with
And one in capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with
Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified.
6. method of quality control as claimed in claim 5, it is characterised in that also include：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, make again
The reaction member is coated with the proteantigen probe, the proteantigen probe includes and the proteantigen pair
The protein antibody answered；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected
The other end of member measures the size of current of response signal, and obtains to be checked relative to the phase place change of pumping signal, and with this
The data message of reaction member is surveyed, the data message includes impedance, electrical conductivity, resistive component, inductive component and electric capacity point
One in amount or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with
Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified；If differing
Cause, repeat the above steps, until the reaction member to be detected is qualified.
7. method of quality control as claimed in claim 6, it is characterised in that reuse the proteantigen probe coating
The step of reaction member to be detected, includes：
First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to each
Reaction member does the scanning of frequency electrical conductivity, and recording responses electric current and phase difference simultaneously select several values in scanning result, as
The characteristic parameter CS of the reaction member to be detected0；
The first liquid is poured out, the proteantigen probe solution of the first concentration is placed in inside reaction member to be detected, by 0-120
Hour, relative humidity is placed in 70-90 space, the proteantigen probe solution includes and institute at 10-40 degrees Celsius
State the corresponding protein antibody solution of proteantigen；First concentration is corresponding with the concentration of the target detection thing；
First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction
Unit does frequency impedance scanning, and recording responses electric current and phase difference simultaneously select several values in scanning result, are used as the reaction
The characteristic parameter CS of unit1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, it is relatively wet by 0-120 hours at 20-30 degrees Celsius
Spend in the space for 70-100 and place；
First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction
Unit does frequency impedance scanning, and recording responses electric current and phase difference simultaneously select several values in scanning result, are used as the reaction
The characteristic parameter CS of unit2；And
The first liquid is poured out, examines thing to be put into reaction to be detected the target of known the first concentration for including proteantigen single
In first, inspection is performed, three coating parameter CS are obtained respectively0、CS1、CS2It is used as the coating of the reaction member under the first concentration
Parameter, obtains each parameter corresponding standing time, temperature and humidity.
8. method of quality control as claimed in claim 7, it is characterised in that pour out the first liquid, by the albumen of the first concentration
Matter antigen probe solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is
Also include after the step of being placed in 70-90 space：
10Hz~10MHz excitation letter is applied to the reaction member to be detected of the proteantigen probe solution containing the first concentration
Number.
9. method of quality control as claimed in claim 7, it is characterised in that reuse the proteantigen probe coating
The step of reaction member to be detected, also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected
Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected
Unit is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected
Unit is cleaned.
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