CN107261061A

CN107261061A - A kind of Chinese medicine composition for treating malignant tumour, preparation method and its preparation

Info

Publication number: CN107261061A
Application number: CN201710489050.4A
Authority: CN
Inventors: 邓圣平
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-06-23
Filing date: 2017-06-23
Publication date: 2017-10-20
Anticipated expiration: 2037-06-23
Also published as: CN107261061B; WO2018233213A1; TW201904594A; HK1247513A2

Abstract

The invention discloses a kind of Chinese medicine composition, its preparation method and its preparation for treating malignant tumour.The bulk drug of the Chinese medicine composition is constituted：Common Camptotheca Fruit, brucea fruit oil, Chinese blister beetle (system), the root of langdu (system), the rhizoma bolbostemmae, Glabrous Sarcandra Herb, selfheal, curcuma zedoary, the Radix Astragali, the red sage root, Poria cocos, pseudo-ginseng, Phellinus, dandelion, gynostemma pentaphylla, radix glycyrrhizae, tealeaves.The medicine can effectively suppress the existence of cancer cell, the regulation acid-base imbalance internal because malignant tumour causes and redox state are unbalance when for treating malignancy disease, maintain the state stability of vivo environment, promote normal cell metabolism, with good effect, the small advantage of toxic side effect.

Description

Traditional Chinese medicine composition for treating malignant tumor, preparation method and preparation thereof

Technical Field

The invention relates to a traditional Chinese medicine composition, in particular to a medicine composition for treating malignant tumors, and also relates to a preparation method and a preparation thereof.

Background

Malignant tumors are now called cancers (cancer), and it is relatively benign. Cancer is a disease caused by the abnormal regulation and over proliferation of body cells, and is often represented by local masses formed by abnormal proliferation of cells in local tissues. Hyperproliferative cells are called cancer cells, and cancer cells often invade surrounding tissues and can even be transferred to other parts of the body through the body circulation system and/or lymphatic system, namely cancer metastasis. There are many types of cancer, and the severity of the disease depends on the location of the cancer cells, whether metastasis occurs. Once confirmed, treatment is often performed today in combination with surgery, chemotherapy, and radiation therapy. If the cancer is untreated, the end result will generally lead to death.

Cancer is a large group of diseases caused by multiple causes, multiple stages and multiple mutations of normal cells of the body. The etiology of cancer generally includes: the organism can cause the canceration of normal cells of the body under the action of various carcinogenic substances such as environmental pollution, chemical pollution (chemical toxin), ionizing radiation, free radical toxin, microorganism (bacteria, fungi, virus and the like) and metabolic toxin thereof, genetic characteristics, endocrine imbalance, immune dysfunction and the like, and carcinogenic factors. Specifically, the method comprises the following steps:

genetic factors: cancer is not currently a clear inherited disease, but there is increasing evidence that cancer is indeed predisposed to inheritance, particularly in the liver. Studies have shown that genes are the material basis for influencing the inheritance of cancer. Among the genes in the normal human body, one is called protooncogene and the other is called cancer suppressor gene. Protooncogenes, also known as oncogenes, if mutated, can cause genetic disorders that, under the action of internal and external factors, transform normal cells into cancerous cells; the cancer suppressor gene can inhibit or antagonize the function and action of cancer gene, and if the cancer suppressor gene is directly mutated, the cancer suppressor gene can also cause cell growth disorder to cause canceration. Therefore, the protooncogene and the cancer suppressor gene can be said to be contradictory genes, and if either the protooncogene or the cancer suppressor gene conflicts, cancer may occur, which is also a potential factor for causing the inheritance of cancer;

chemical factors are as follows: this is the major cause of malignant tumor development that is currently discovered. More than 1000 chemical substances have been found to have carcinogenic effect in the medical field, and more than thirty chemical substances have close relationship with malignant tumor of human, such as polycyclic aromatic hydrocarbons of 3.4-benzopyrene, mustard gas, benzidine, aniline, azo compounds, nitrosamine compounds, chromium, nickel, zinc, cadmium, arsenic, molybdenum, rod, cotton, vinyl chloride, benzene, etc., which are common carcinogenic substances; there are also some chemical substances that have potential carcinogenic effects on humans, such as pesticides, household chemical cleaners, disinfectants, etc.; in addition, studies have shown that some medical drugs, such as immunosuppressants, hormonal drugs, etc., also have carcinogenic activity. Different chemical substances can cause different malignant tumors, for example, lung cancer can be caused by long-term large-dose chromium contact, lung cancer and nasopharyngeal carcinoma can be caused by nickel contact, skin cancer and liver cancer can be caused by arsenic contact, prostate cancer can be caused by cadmium contact, and scrotal cancer and skin cancer can be easily caused by 3.4-benzopyrene which is mainly eaten in coal ash and coal tar;

physical factors: studies have shown that the main carcinogenic effects in humans and animals are ionizing radiation, X-rays, microwaves, electromagnetic waves, radioactive substances, thermal radiation, sunlight, ultraviolet rays, mechanical stimuli, burning, wounds, etc. Human beings are easy to suffer from leukemia, osteosarcoma, thyroid cancer and other diseases by ionizing radiation, can induce skin squamous cell carcinoma by larger dose of X-ray contact or large dose of ultraviolet irradiation, can easily obtain lung cancer and leukemia by long-term contact with radioactive substances or living in radioactive substance polluted areas for a long time, and easily induce esophageal cancer by eating overheated food for a long time and over-hard food;

biological factors: biological carcinogenic factors can be divided into two types, one is virus, and the other is mould; some viruses can induce malignant tumors after they are latent in human body under certain conditions. The malignant tumor related to virus mainly includes nasopharyngeal carcinoma, cervical carcinoma, leukemia, liver cancer, etc. Grains, nuts, vegetables and the like which are eaten in daily life are easily polluted by various moulds such as kojitoxin, fusarium, alternaria, aspergillus versicolor and the like, so that liver cancer and gastric cancer are induced;

HPV human papillomavirus factors: HPV human papillomaviruses are generally benign, but cause lesions, as well as respiratory papillomatosis (respiratory papillomatosis) and various other cancers;

lifestyle effects: studies have shown that poor lifestyle has a definite impact on cancer pathologies such as smoking, diet, exercise, alcohol, sun exposure, and venereal disease. The occurrence of cancer is also related to the amount of melatonin in the body, and when a long time is needed to stay in a bright environment, the expression amount of melatonin is low, while the incidence rate of cancer is high;

diffusion effect: while the tumor can temporarily solve the self-feeding problem by building blood vessels during its growth, the rapid growth also pushes it to the resource and space starved margin, at which point the tumor spreads and begins to invade the surrounding normal tissue (cancer infiltration) and metastasize to the distant end through blood vessels (cancer metastasis).

Currently, the primary means of treating cancer relies on western medicine, often in combination with surgery, chemotherapy and/or radiotherapy. However, the above treatment methods can also damage healthy cells while killing cancer cells, and patients need to suffer from pain and adverse side effects caused by operations, chemotherapy and radiotherapy, so that the problems of low treatment overdue rate and easy recurrence in western medicine treatment generally exist, and the problem of high medical expense also exists. In recent years, medical attention has been gradually paid to the treatment of malignant tumors by the traditional Chinese medicine therapy, which is increasingly applied clinically due to the advantages of small side effect and low treatment cost and obtains better curative effect.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for treating malignant tumors and further discloses a preparation method thereof.

In order to solve the technical problems, the invention provides a traditional Chinese medicine composition for treating malignant tumors, which comprises the following raw medicines:

preferably, the traditional Chinese medicine composition comprises the following raw medicines:

the invention also discloses a clinically acceptable preparation prepared from the traditional Chinese medicine composition for treating malignant tumors by adding conventional auxiliary materials according to a conventional process.

Preferably, the preparation comprises capsules, granules, patches, injections, mixtures or pills.

The adjuvants include at least one of medical starch, lactose, beta-cyclodextrin, magnesium stearate, oleum Camelliae Japonicae, salicylic acid, white vaseline, glacial acetic acid, solid paraffin, Mel, calx, and distilled water.

The invention also discloses a preparation method of the preparation, which comprises the steps of uniformly mixing selected amounts of common camptotheca fruit, brucea javanica oil, cantharis, radix euphorbiae lantu, bolbostemma, glabrous sarcandra herb, common selfheal fruit-spike, zedoary, astragalus, salvia miltiorrhiza, tuckahoe, pseudo-ginseng, phellinus igniarius, dandelion, fiveleaf gynostemma herb, liquorice and tea leaves, adding conventional auxiliary materials, and preparing the clinically acceptable preparation according to the conventional process.

Preferably, the method for preparing the preparation comprises the following steps:

s1, placing a selected amount of Mylabris and Oryza Glutinosa in a hot pot, heating with slow fire, parching until Oryza Glutinosa turns yellowish brown, taking out, removing Oryza Glutinosa, cooling, removing head, wings and feet of Mylabris, and keeping;

s2, taking a selected amount of radix Euphorbiae Fischerianae tablets, adding rice vinegar, stirring, placing into a pot, parching with slow fire, taking out, cooling, storing in a drying container, and sealing for use;

s3, mixing processed Mylabris and radix Euphorbiae Fischerianae tablet with selected amount of fructus Camptothecae Acuminatae, oleum fructus Bruceae, rhizoma Bolbostematis, herba Pileae Scriptae, Prunellae Spica, Curcumae rhizoma, radix astragali, Saviae Miltiorrhizae radix, Poria, Notoginseng radix, Phellinus Linteus, herba Taraxaci, herba Gynostemmatis, Glycyrrhrizae radix and folium Camelliae sinensis, adding conventional adjuvants, and making into clinically acceptable preparation according to conventional process.

In the step S1, the mass ratio of the cantharis to the sticky rice is 1: 2; in the step S2, the mass ratio of the added radix euphorbiae lantu tablet to the rice vinegar is 10: 3.

most preferably, the preparation method comprises the following steps:

s3, mixing processed Mylabris and radix Euphorbiae Fischerianae tablet with selected amount of fructus Camptothecae Acuminatae, rhizoma Bolbostematis, herba Pileae Scriptae, Prunellae Spica, Curcumae rhizoma, radix astragali, Saviae Miltiorrhizae radix, Poria, Notoginseng radix, Phellinus Linteus, herba Taraxaci, herba Gynostemmatis, Glycyrrhrizae radix and folium Camelliae sinensis, soaking in water, heating to boil and decocting, mixing the decoction with selected amount of oleum fructus Bruceae, separating solid and liquid, collecting filtrate, concentrating with slow fire to obtain concentrated solution with specific gravity of 1.30-1.35, oven drying, adding conventional adjuvants, and making into clinically acceptable capsule; or,

s3, mixing the processed cantharis and radix euphorbiae lantu tablets with selected amount of fructus camptothecae acuminatae, rhizoma bolbostemmae, glabrous sarcandra herb, selfheal, rhizoma zedoariae, astragalus membranaceus, salvia miltiorrhiza, poria cocos, pseudo-ginseng, phellinus igniarius, dandelion, gynostemma pentaphylla, liquorice and tea leaves uniformly, crushing into coarse particles, soaking in water, heating and decocting, collecting decoction, adding selected amount of oleum fructus bruceae, evaporating with slow fire to an evaporation solution with a specific gravity of 0.9-1.1, cooling, adding 60 wt% ethanol, fully mixing, precipitating, taking supernate, recovering ethanol under reduced pressure, concentrating to a concentrated solution with a specific gravity of 1.2-1.5, evaporating with slow fire to a thick high state, drying, adding conventional auxiliary materials, and preparing clinically acceptable granules; or,

s3, pulverizing processed Mylabris and radix Euphorbiae Fischerianae tablet, and selected amount of fructus Camptothecae Acuminatae, rhizoma Bolbostematis, herba Pileae Scriptae, Prunellae Spica, Curcumae rhizoma, radix astragali, Saviae Miltiorrhizae radix, Poria, Notoginseng radix, Phellinus Linteus, herba Taraxaci, herba Gynostemmatis, Glycyrrhrizae radix and folium Camelliae sinensis, and sieving with 200 mesh sieve; decocting Mel in porcelain pot, adding oleum fructus Bruceae, stirring, adding above powders, cooling, and making into clinically acceptable pill; or,

s3, mixing processed Mylabris and radix Euphorbiae Fischerianae tablet with selected amount of fructus Camptothecae Acuminatae, rhizoma Bolbostematis, herba Pileae Scriptae, Prunellae Spica, Curcumae rhizoma, radix astragali, Saviae Miltiorrhizae radix, Poria, Notoginseng radix, Phellinus Linteus, herba Taraxaci, herba Gynostemmatis, Glycyrrhrizae radix, and folium Camelliae sinensis, soaking in water, decocting under heating, collecting decoction, adding selected amount of oleum fructus Bruceae oil, mixing, performing solid-liquid separation, collecting filtrate, concentrating with slow fire to obtain concentrated solution with specific gravity of 1.30-1.35, adding 0.9% sodium chloride solution, and making into clinically acceptable Chinese medicinal injection.

The mass ratio of the added honey to the total amount of the traditional Chinese medicine raw materials is (3-6): 100.

the invention also discloses a patch for treating malignant tumor, which comprises the following raw material medicaments:

1-30 parts of common camptotheca fruit, 1-30 parts of brucea fruit oil, 0.1-0.9 part of cantharides

1-30 parts of radix euphorbiae lantu, 1-30 parts of rhizoma bolbostemmae, and 1-30 parts of glabrous sarcandra herb.

The invention also discloses a method for preparing the patch for treating malignant tumor, which comprises the following steps:

s3, pulverizing processed Mylabris and radix Euphorbiae Fischerianae tablet, and selected amount of fructus Camptothecae Acuminatae, rhizoma Bolbostematis, and herba Pileae Scriptae in pulverizer, and sieving with 200 mesh sieve;

s4, putting quicklime into a container filled with clear water, reacting to obtain limewater, taking out the limewater after the limewater is settled for fifteen days, filling into the container for standby, adding selected amounts of oleum fructus brucease and raw camellia oil into the container filled with limewater, stirring into paste, and finally adding the fine powder prepared in the step S3 to obtain the clinically acceptable patch.

The mass ratio of the quicklime and the clear water added in the step S4 is 1:5, the mass ratio of the taken precipitated limewater to the camellia oil is 1: 1.

the traditional Chinese medicine composition disclosed by the invention is prepared by selecting seventeen traditional Chinese medicine raw materials and re-composing on the basis of a large number of early experimental studies under the guidance of the traditional Chinese medicine theory and in combination with the research results of modern medicine. The traditional Chinese medicine composition takes the common camptotheca fruit, the brucea javanica, the cantharis and the radix euphorbiae lantu as monarch drugs and mainly attacks the noxious evil; the ten medicines of rhizoma bolbostemmae, glabrous sarcandra herb, common selfheal fruit-spike, zedoary, membranous milkvetch root, danshen root, Indian buead, sanchi, phellinus igniarius and mongolian dandelion herb are taken as ministerial medicines to achieve the effects of clearing away heat and toxic materials, removing accumulation, activating blood and dissolving stasis; gynostemma pentaphylla, liquorice and tea are used as adjuvant drugs to achieve the effects of detoxifying and attacking toxin, strengthening body resistance and eliminating evil. Wherein,

and (3) common camptotheca fruit: the camptothecin can change the cancer cells in such morphological ways as degeneration, necrosis, nuclear condensation, dense staining, chromatin condensation, cytoplasmic vacuole and the like, can change the ultrastructure of the cancer cells and host liver cells, and finally leads the cancer cells to be dissolved and destroyed to promote cell death;

brucea javanica: the brucea javanica oil has the most obvious effect on the brucea javanica oil, and the brucea javanica alcohol, brucea javanica alcohol and brucea javanica oil A, D, E have marginal inhibition on the activity of P388 lymphocytes, leukemia DNA polymerase, RNA polymerase, thymidylate synthase (thymidylate-theta), dihydrofolate reductase, phosphoribosyl pyrophosphate aminotransferase and cathepsin, and inhibit RNA and protein synthesis. Brucea javanica picrol has the strongest effect and can inhibit the proliferation of P388 cells and the activity of hexokinase, phosphofructokinase and succinate dehydrogenase in the cells. The daylily lignan A, brucine D, E, brucine A, brucine A, H, F, I, J, L, P, brucine alcohol and dehydrobrucine alcohol contained in the brucea javanica have the activity of resisting P388 lymphatic leukemia. The brucea javanica glycoside AB also has an inhibiting effect on W256 and ECA ehrlich ascites carcinoma;

cantharis: the cantharidin is an active ingredient of cantharis, has strong affinity to liver and cancer cells, can firstly inhibit the synthesis of cancer cell protein, then influence the synthesis of RNA and DNA, and finally inhibit the growth and division of cancer cells;

radix euphorbiae ebracteolatae: bitter, pungent and neutral in taste, has the effects of expelling water and eliminating phlegm, breaking accumulation and killing pests, and the genidi wood in the radix euphorbiae lantu has stronger inhibiting effect on various tumors;

paniculate Bolbostemma rhizome: the tubeimoside I has the effects of clearing heat, reducing phlegm, resolving masses and removing toxicity;

glabrous sarcandra herb: the medicine is pungent and bitter in taste and mild in nature, has the effects of dispelling wind, removing dampness, promoting blood circulation, removing blood stasis, and clearing away heat and toxic materials, and the volatile oil of the glabrous sarcandra herb has certain inhibiting effects on 615 cells of autogenous leukemia, TM755, 615 pulmonary adenosis, 615 white hair breast cancer, AL771 spontaneous ascites type, ehrlich ascites carcinoma, 180 sarcoma, 37 sarcoma and 256 Wacker carcinoma;

selfheal: the beverage is bitter, pungent and cold in taste, has the effects of clearing liver-fire, resolving stagnation, resolving hard mass, relieving swelling and removing toxic substances, has obvious cytotoxicity on cells P388 and L1210 and human tumor cells A-549 due to ursolic acid contained in the selfheal, and has inhibiting effect on Ehrlich ascites tumor and sarcoma S180;

zedoary turmeric: the product is pungent, bitter and warm in taste, has the effects of breaking blood, promoting qi circulation, removing food retention, relieving pain, resisting tumor, killing liver cancer cells cultured in vitro, and inhibiting RNA polymerase activity, so that the DNA absorption spectrum shift and fluorescence intensity are increased;

astragalus root: the astragalus polysaccharide has synergistic effect on the activation of the cancer ascites lymphocytes, CTL cells in the cancer ascites of a user are easily activated by IL-2, the activation time is shortened, and the amplification times are large; in addition, the traditional Chinese medicine composition has an obvious promotion effect on the secretion of TNF (tumor necrosis factor) in vitro by peripheral blood mononuclear cells of normal people and tumor patients, has an enhancement effect on TNF-a and TNF-b, can remarkably enhance the in vitro killing activity of lung cancer infiltrating lymphocytes (TIL), and has a synergistic positive regulation effect on the TIL cells when being used together with ginsenoside;

red sage root: the tanshinone IIA has the effects of cooling blood, reducing swelling, promoting blood circulation, removing blood stasis, nourishing blood, soothing nerves and promoting tissue repair and regeneration, and can inhibit HL-60 cell synthesis by influencing oncogene expression and DNA polymerase activity so as to inhibit cell proliferation and induce cell differentiation;

tuckahoe, poria cocos: the pachyman has obvious inhibition effect on slow-growing type transplanted tumor, and an anti-tumor action mechanism of the pachyman mainly enhances the immune function of an organism, activates an immune monitoring system, improves the immunity of the organism to a cancer cell specific antigen, exerts the anti-cancer capability of the organism, and leads the activated complement to synergistically kill tumor cells by influencing M phi, lymphocytes, humoral factors and the like;

pseudo-ginseng: the ginseng glycosides Rb1, Rd, Rh1 and Rh2 in the pseudo-ginseng can inhibit cultured tumor cells, Rh1 directly acts on cell membranes, and Rh2 can ensure that cancer cells are redifferentiated, induced and reversely converted into non-cancer cells;

phellinus igniarius: slightly bitter and cold in taste, has anticancer effect, and can enhance immunity, induce cancer cell to die, and inhibit proliferation and metastasis of cancer cell;

dandelion: is bitter, sweet and cold in nature, and has the effects of clearing away heat and toxic materials, eliminating stagnation, relieving swelling, eliminating dampness and promoting urination;

gynostemma pentaphylla: sweet taste and warm nature, has the effects of tonifying deficiency, moistening dryness, dispelling pathogenic wind, promoting blood circulation, relieving swelling, removing toxic substance, and resisting tumor, and herba Gynostemmatis can prevent canceration of normal cells, promote self-healing of cells, and guide canceration cells to recover normal. The gynostemma pentaphylla has obvious influence on the CO irradiation effect of human gastric adenocarcinoma (SGC-7901) cells, shows that the survival rate of the cells is improved, the cells in the S phase are obviously increased, and the proportion of the cells in the G1 phase and the G2 phase is reduced; in the gynostemma pentaphylla, sapogenox, can directly kill S180 intratumoral cells. The gypenoside XX1 can significantly inhibit the growth of liver cancer cells in vitro. The gypenoside has inhibitory effect on in vitro cultured lung cancer, metrocarcinoma, and melanoma cells. The panaxadiol and protopanaxadiol in the gynostemma pentaphylla have certain antitumor activity, and the gynostemma pentaphylla polysaccharide has strong inhibiting and killing effects on tumor cells. The gynostemma pentaphylla has the functions of inhibiting tumor cells, proliferating and enhancing immune function, and is mainly reflected in blocking mitosis of cancer cells, interfering the DNA synthesis process and improving mutation capacity of the cancer cells. GPS can make the frequency of sister chromosome interchange increased and obviously reduced caused by cyclophosphamide. The gynostemma pentaphylla can reduce DNA synthesis of rectal adenocarcinoma cells, reduce the number of nuclear divisions and cause cell degeneration and necrosis;

licorice root: sweet taste and mild nature, and has the effects of invigorating qi, invigorating middle warmer, relieving spasm and pain, moistening lung for arresting cough, clearing pathogenic fire, removing toxic substance, and harmonizing drug property;

tea leaf: the Green Tea Polyphenol (GTP) can inhibit aflatoxin B (AFB), benzopyrene BP and the like, regulate immunity, improve the hematopoietic function of marrow damaged by cyclophosphamide, increase the number of white blood cells and red blood cells, and have the function of gene regulation.

The traditional Chinese medicine composition disclosed by the invention is used for carrying out clinical experiments on 56 patients who are diagnosed with advanced primary liver cancer, the symptoms in the treatment effect are completely relieved by 12 patients, partially relieved by 20 patients and stabilized by 22 patients, and the total effective rate is up to 96.4%, so that the traditional Chinese medicine composition disclosed by the invention can effectively control the growth of tumors, inhibit the diffusion of cancer cells, and simultaneously can improve the symptoms of pain in liver regions, body weakness, inappetence and the like of the patients, thereby improving the survival quality of the patients and prolonging the life cycle of the patients. Besides, the medicine of the invention has ideal treatment effect on other tumor diseases such as lung cancer, lymph cancer, gastric cancer, esophagus cancer, bladder cancer, ovarian cancer, prostatic cancer, pancreatic cancer and the like and the rehabilitation treatment of tumor patients who have been subjected to surgery, radiotherapy and chemotherapy.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below. This invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete and will fully convey the concept of the invention to those skilled in the art, and the present invention will only be defined by the appended claims.

Example 1:

the invention relates to a Chinese medicinal composition (capsule preparation) for treating malignant tumor

The traditional Chinese medicine composition of the capsule for treating malignant tumor comprises the following components:

the preparation method of the capsule for treating malignant tumor comprises the following steps:

s1, mixing the components in a mass ratio of 1: 2, placing the cantharis and the sticky rice in a hot pot, heating with slow fire, stir-frying until the sticky rice is yellow brown, taking out, removing the sticky rice, cooling, removing the head, the wings and the feet of the cantharis for standby;

s2, taking radix euphorbiae lantu tablets, adding rice vinegar 0.3 times of the weight of the radix euphorbiae lantu tablets, uniformly stirring, moistening thoroughly, placing in a pot, frying with slow fire, taking out, cooling, storing in a drying container, and sealing for later use;

s3, taking 0.8g of cantharis and 10g of radix euphorbiae lantu tablets after being processed in the steps S1 and S2, 12g of common camptotheca fruit, 6g of rhizoma bolbostemmae, 6g of glabrous sarcandra herb, 12g of common selfheal fruit-spike, 9g of zedoary, 9g of astragalus membranaceus, 8g of salvia miltiorrhiza, 9g of poria cocos, 10g of pseudo-ginseng, 6g of phellinus igniarius, 12g of dandelion, 15g of gynostemma pentaphylla, 6g of liquorice and 6g of tea, adding 500ml of water, soaking for 30 minutes, heating to boiling, decocting for 90 minutes, filtering out decoction liquid, decocting dregs of a decoction for 2 times according to the method, collecting each decoction liquid, adding 12g of brucea javanica oil, uniformly stirring, centrifugally separating, concentrating the separation liquid by slow fire to obtain concentrated liquid with the specific gravity of 1:1 adding beta cyclodextrin to prepare the capsule for treating malignant tumor.

Example 2:

the invention relates to a Chinese medicinal composition (granule) for treating malignant tumor

The traditional Chinese medicine composition of the granules for treating malignant tumors comprises the following components:

the preparation method of the granules for treating malignant tumors comprises the following steps:

s3, taking 0.5g of cantharis, 9g of radix euphorbiae lantu tablets, 12g of common camptotheca fruit, 12g of paniculate bolbostemma rhizome, 15g of glabrous sarcandra herb, 9g of common selfheal fruit-spike, 9g of zedoary, 9g of astragalus root, 5g of salvia miltiorrhiza, 6g of poria cocos, 6g of pseudo-ginseng, 6g of phellinus igniarius, 6g of dandelion, 8g of gynostemma pentaphylla, 9g of liquorice and 12g of tea, crushing the raw materials into coarse particles, placing the coarse particles in a container, adding 500ml of water for soaking for 30 minutes, heating to boil, decocting for 90 minutes, filtering out decoction liquid, decocting dregs for 3 times according to the method, collecting each decoction liquid, adding 12g of brucea javanica oil, evaporating the mixture with slow fire until the specific gravity is 1.0, adding 1.5 times of 60% ethanol for fully mixing after cooling, standing overnight, taking supernatant (filtering if necessary), washing precipitates with 2 times of 60% ethanol to obtain washing liquid, merging the washing liquid and supernatant, recovering the washing liquid under, concentrating to obtain concentrated solution with the specific gravity of 1.2, standing for 36 hours, filtering, evaporating with slow fire to obtain thick liquid, drying, and mixing the concentrated solution with the water according to the mass ratio of 1:1 adding medical starch to prepare the Chinese medicinal granule for treating malignant tumor.

Example 3:

the invention relates to a Chinese medicinal composition (patch) for treating malignant tumor

The traditional Chinese medicine composition of the patch for treating malignant tumors comprises the following components:

camptotheca fruit 12g oleum fructus Bruceae 12g Mylabris 0.8g

Radix Euphorbiae Fischerianae (preparata) 12g, rhizoma Bolbostematis 18g, herba Pileae Scriptae 15 g.

The preparation method of the patch for treating malignant tumor of the embodiment comprises the following steps:

s3, taking 0.8g of cantharis, 12g of radix euphorbiae lantu tablets, 12g of common camptotheca fruit, 18g of rhizoma bolbostemmae and 15g of glabrous sarcandra herb which are processed in the steps S1 and S2, crushing the raw materials in a crusher, sieving the crushed materials with a 200-mesh fine sieve, and sieving the crushed materials into fine powder for later use;

s4, putting quicklime into a container filled with clear water, wherein the mass ratio of the quicklime to the clear water is 1:5, reacting to obtain lime water, taking out 12g of settled lime water after the lime water settles for fifteen days, filling the lime water into the container for standby, adding 12g of raw camellia oil and 12g of oleum fructus brucease into the container filled with lime water, stirring into paste, and finally adding the fine powder prepared in the step S3 to prepare the patch for treating malignant tumors.

Example 4:

the invention relates to a traditional Chinese medicine composition (big honeyed pill) for treating malignant tumor

The big honeyed pill traditional Chinese medicine composition for treating malignant tumor comprises the following components:

the preparation method of the big honeyed pill for treating malignant tumor comprises the following steps:

s3, taking 0.6g of cantharis and 6g of radix euphorbiae lantu tablets after being processed by the steps S1 and S2, 9g of common camptotheca fruit, 9g of rhizoma bolbostemmae, 9g of glabrous sarcandra herb, 12g of common selfheal fruit-spike, 6g of zedoary, 12g of astragalus root, 6g of red-rooted salvia root, 6g of Indian buead, 10g of pseudo-ginseng, 8g of phellinus igniarius, 6g of dandelion, 9g of fiveleaf gynostemma herb, 9g of liquorice and 9g of tea leaves, putting the raw materials into a grinder to be ground, and sieving;

s4, putting 6g of raw honey into a porcelain pot, decocting until 2-3 cm of shredded tobacco can be drawn by hands, adding 9g of oleum fructus bruceae, stirring uniformly, adding the powder prepared in the step S3, mixing uniformly, cooling, and preparing the oral big honeyed pill for treating malignant tumor.

Example 5:

the invention relates to a traditional Chinese medicine composition (small honeyed pill) for treating malignant tumor

The composition of the small honeyed pill for treating malignant tumor in the embodiment comprises:

the preparation method of the small honeyed pill for treating malignant tumor comprises the following steps:

s3, taking 0.8g of cantharis and 6g of radix euphorbiae lantu tablets after being processed by the steps S1 and S2, 10g of common camptotheca fruit, 15g of rhizoma bolbostemmae, 15g of glabrous sarcandra herb, 15g of common selfheal fruit-spike, 7g of zedoary, 7g of astragalus root, 7g of red-rooted salvia root, 7g of tuckahoe, 7g of pseudo-ginseng, 7g of phellinus igniarius, 15g of dandelion, 15g of gynostemma pentaphylla, 7g of liquorice and 7g of tea leaves, putting the raw materials into a grinder to be ground, and sieving the ground materials into powder by;

s4, placing 7g of raw honey into a porcelain pot, decocting until 2-3 cm of shredded tobacco can be drawn by hands, adding 10g of oleum fructus bruceae, stirring, adding the powder prepared in the step S3, mixing, cooling, and making into oral honeyed pill for treating malignant tumor.

Example 6:

the invention relates to a traditional Chinese medicine composition (water-honeyed pill) for treating malignant tumor

The traditional Chinese medicine composition of the water-honeyed pill for treating malignant tumor comprises the following components:

the preparation method of the water-honeyed pill for treating malignant tumor comprises the following steps:

s3, taking 0.2g of cantharis and 4g of radix euphorbiae lantu tablets after being processed by the steps S1 and S2, and taking 4g of common camptotheca fruit, 4g of rhizoma bolbostemmae, 4g of glabrous sarcandra herb, 5g of common selfheal fruit-spike, 4g of zedoary, 6g of astragalus root, 8g of red-rooted salvia root, 12g of tuckahoe, 11g of pseudo-ginseng, 4g of phellinus igniarius, 5g of dandelion, 5g of fiveleaf gynostemma herb, 9g of liquorice and 9g of tea leaves, putting the raw materials into a grinder to be ground, and sieving the;

s4, placing 5g of raw honey into a porcelain pot, decocting until 2-3 cm of shredded tobacco can be drawn by hands, adding 4g of oleum fructus bruceae, stirring, adding the powder prepared in the step S3, mixing, cooling, and making into oral water-honeyed pill for treating malignant tumor.

Example 7:

the invention relates to a Chinese medicinal composition (injection) for treating malignant tumor

The injection for treating malignant tumor in the embodiment comprises the following Chinese medicinal compositions:

the preparation method of the injection for treating malignant tumor comprises the following steps:

s3, taking 0.5g of cantharis and 6g of radix euphorbiae lantu tablets after being processed in the steps S1 and S2, 9g of common camptotheca fruit, 9g of rhizoma bolbostemmae, 9g of glabrous sarcandra herb, 9g of common selfheal fruit-spike, 12g of curcuma zedoary, 6g of astragalus membranaceus, 8g of salvia miltiorrhiza, 6g of poria cocos, 10g of pseudo-ginseng, 8g of phellinus igniarius, 12g of dandelion, 8g of gynostemma pentaphylla, 7g of liquorice and 7g of tea, adding 500ml of water, soaking for 30 minutes, heating to boiling and decocting for 90 minutes, filtering out the decoction liquid, decocting the medicine dregs for 2 times according to the method, collecting each decoction liquid, adding 9g of brucea javanica oil, uniformly stirring, centrifugally separating, concentrating the separation liquid to a concentrated liquid with the specific gravity of 1.35 by slow fire, then adding a sodium chloride solution with the concentration of 0.9% according to.

Examples of the experiments

Experimental example 1 therapeutic Effect on liver cancer

Inhibition experiment for hepatoma tumor cell line

1. Material

1.1 medicine: weighing 12g of common camptotheca fruit, 0.8g of cantharides (prepared), 10g of radix euphorbiae lantu tablets (prepared), 6g of paniculate bolbostemma rhizome, 6g of glabrous sarcandra herb, 12g of common selfheal fruit-spike, 9g of zedoary, 9g of astragalus root, 8g of salvia miltiorrhiza, 9g of poria cocos, 10g of pseudo-ginseng, 6g of phellinus igniarius, 12g of dandelion, 15g of gynostemma pentaphylla, 6g of liquorice and 6g of tea, adding 500ml of water for soaking for 30 minutes, heating to boil, decocting for 90 minutes, filtering decoction liquid, decocting dregs of a decoction for 2 times according to the method, collecting each decoction liquid, adding 12g of brucea javanica oil, uniformly stirring, centrifugally separating, and concentrating the separation liquid with slow fire to obtain concentrated liquid with.

1.2 animals: KM mice, body weight 20. + -.3 g.

1.3 tumor strains: liver cancer HepS 22.

2. Experimental methods

Taking tumor-bearing subcultured mice, removing cervical vertebrae, killing, cutting skin, selecting cancer tissues with good tumor growth and no necrosis or liquefaction, and carrying out the following steps of 1: 3 adding sterile normal saline, preparing cancer cell suspension by using a tissue homogenizer, and inoculating 0.2ml of the cancer cell suspension subcutaneously in the armpit of the experimental mouse. The day after inoculation, the groups were randomized, and 12 were administered.

The experiment is designed to be three experimental groups, a model group and a control group for high, medium and low dose administration.

Wherein the dosage of the high-dose experimental group is 1g/kg body weight, the dosage of the medium-dose experimental group is 0.5g/kg body weight, and the dosage of the low-dose experimental group is 0.1g/kg body weight.

The control group was given cyclophosphamide at 20mg/kg body weight.

The model group was given sterile saline 1g/kg body weight.

Gavage was administered once daily and sacrificed 10 days after administration.

Weighing, picking tumors, weighing, and calculating the tumor inhibition rate according to the following calculation formula:

the tumor inhibition rate (100%) was (1-mean tumor weight in dosing group/mean tumor weight in model group) × 100%.

3. Statistical method

Statistical analysis is carried out on the data by adopting SPSS17.0 software, the measurement data is expressed by X +/-S, and the indexes before and after treatment are compared by adopting t test; the count data was examined by X2. P < 0.05 indicates that the difference is statistically significant.

4. Results of the experiment

Table 1: experimental result of the drug of the invention on HepS22 solid tumor

Administration of each group	Number of mice	Mean tumor weight (g) X. + -. SD	Tumor inhibition ratio (100%)
				Model set	12	3.14±0.16	-
Control group	12	1.57±0.21	50.00
				High dose group	12	1.44±0.28	54.14
Middle dose group	12	1.56±0.32	50.32
				Low dose group	12	1.68±0.44	46.50

Comparison with model groups: p is less than 0.05; comparison with the control group: p is less than 0.05

The experimental results show that: the traditional Chinese medicine composition has an obvious inhibition effect on experimental liver tumors of mice. The tumor inhibition rate of the high-dose group of the traditional Chinese medicine composition is obviously higher than that of a western medicine cyclophosphamide control group, and the traditional Chinese medicine composition has significant difference.

II, clinical experiments of the traditional Chinese medicine composition:

the clinical trial report for the treatment of 87 primary liver cancer patients during 1 month to 2016 12 months in 2014 is as follows:

1. general data

The 56 patients selected in the study are confirmed patients of advanced primary liver cancer, and all the patients meet the diagnosis standard related to the primary liver cancer in the clinical diagnosis and treatment guideline. Of the 56 patients, 33 men and 23 women had an average age of 44.

And (3) clinical staging: 35 cases in II stage and 21 cases in III stage.

2. Method of treatment

The patient takes the capsule prepared in the embodiment 1 of the invention 2 capsules each time, 3 times a day, 1 month is a treatment course, and 3 treatment courses are continuously taken.

3. The judgment standard of the curative effect is as follows:

and (3) complete alleviation: the tumor tissue completely disappears, and the serum alpha-fetoprotein is restored to a normal range;

partial mitigation: the tumor tissue is reduced by more than 50 percent, and the serum alpha-fetoprotein is restored to a normal range;

and (3) stabilizing: the reduction or enlargement of the tumor tissue is less than 25%;

deterioration: tumor tissue enlargement was greater than 20%.

4. The experimental results are as follows:

improvement of clinical symptoms: the improvement rate of the pain of the liver area is 82.5 percent, the improvement rate of the fatigue and weakness is 76.9 percent, and the improvement rate of the inappetence is 83.2 percent.

The treatment effect is as follows: complete remission was 12 cases, accounting for 21.4% of total cases; partial remission was 20 cases, accounting for 35.7% of total cases; the number of stable 22 cases accounts for 39.3 percent of the total cases; the exacerbations were 2 cases, accounting for 3.6% of the total cases. The total effective rate is 96.4%.

The experimental results show that: the traditional Chinese medicine composition can effectively control the growth of tumors, inhibit the diffusion of cancer cells, and simultaneously improve symptoms of liver pain, body weakness, inappetence and the like of patients, thereby improving the survival quality of the patients and prolonging the life cycle of the patients.

Besides liver cancer, the medicine of the invention has ideal treatment effect on other tumor diseases such as lung cancer, lymph cancer, stomach cancer, esophagus cancer, bladder cancer, ovarian cancer, prostatic cancer, pancreatic cancer and the like and the rehabilitation treatment of tumor patients who have undergone operations, radiotherapy and chemotherapy.

The clinical treatment effect of other tumors is evaluated in a unified way:

clinical cure: the original symptoms disappear;

the effect is shown: the original symptom score disappears;

improvement: the original disease condition is relieved;

and (4) invalidation: no change in symptom signs.

Experimental example 2

After 32 patients suffering from gastric cancer are treated by using the capsule prepared in the embodiment 1 of the invention for 3-6 courses of treatment, the treatment effects of the 32 patients suffering from gastric cancer are observed, wherein 18 patients are cured, 8 patients are obviously effective, 5 patients are improved, 1 patient is ineffective, and the total effective rate is 96.9%.

Experimental example 3

After 29 patients with lung cancer are treated by the granule prepared in the embodiment 2 of the invention for 3-5 courses of treatment, the treatment effects of the 29 patients with lung cancer are observed, wherein 22 patients are cured, 5 patients with obvious effect are obtained, 1 patient is improved, 1 patient is not effective, and the total effective rate is 95.5%.

Experimental example 4

After 25 patients with bladder cancer are treated by the granule prepared in the embodiment 2 of the invention for 3-5 courses of treatment, the treatment effects of the 25 patients with bladder cancer are observed, wherein 14 patients are cured, 6 patients with obvious effect are obtained, 3 patients are improved, 2 patients are not effective, and the total effective rate is 92.0%.

Experimental example 5

After 18 cases of patients with ovarian cancer are treated by the granule prepared in the embodiment 2 of the invention for 3-5 courses of treatment, the treatment effects of the 18 cases of patients with ovarian cancer are observed, wherein 11 cases are cured, 3 cases are obviously effective, 3 cases are improved, 1 case is ineffective, and the total effective rate is 94.4%.

Experimental example 6

After 16 patients with prostate cancer are treated by using the patch prepared in the embodiment 3 of the invention for 3-5 courses of treatment, the treatment effect of the 16 patients with prostate cancer is observed, wherein 10 patients are cured, 2 patients with obvious effect are obtained, 4 patients are improved, 0 patient is not obtained, and the total effective rate is 100%.

Experimental example 7

After 30 patients with esophageal cancer are treated by the oral big honeyed pill prepared in the embodiment 4 of the invention for 3-6 courses of treatment, the treatment effects of the 30 patients with esophageal cancer are observed, wherein 8 patients are cured, 7 patients have obvious effects, 3 patients are improved, 2 patients are not effective, and the total effective rate is 93.3%.

Experimental example 8

After 23 cases of patients with lymphoma are treated by using the traditional Chinese medicine compound injection prepared in the embodiment 7 of the invention for 3-6 treatment courses, the treatment effects of the 23 cases of patients with lymphoma are observed, wherein 14 cases are cured, 4 cases are obviously effective, 3 cases are improved, 2 cases are ineffective, and the total effective rate is 91.1%.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are intended to be within the scope of the invention.

Claims

1. A traditional Chinese medicine composition for treating malignant tumors is characterized by comprising the following raw medicines:

2. the traditional Chinese medicine composition for treating malignant tumor according to claim 1, which is prepared from the following raw medicines:

3. the traditional Chinese medicine composition for treating malignant tumor according to claim 1 or 2, wherein the traditional Chinese medicine composition comprises the following raw medicines:

4. the Chinese medicinal composition for treating malignant tumor of any one of claims 1-3, adding conventional adjuvants, and making into clinically acceptable preparation by conventional process.

5. The formulation of claim 4, comprising a capsule, granule, patch, injection, cocktail, or pill.

6. A method for preparing the preparation of claim 4 or 5, which is characterized by comprising the steps of uniformly mixing selected amounts of common camptotheca fruit, brucea javanica oil, cantharis, radix euphorbiae lantu, rhizoma bolbostemmae, glabrous sarcandra herb, common selfheal fruit-spike, zedoary, astragalus mongholicus, red-rooted salvia root, poria cocos, pseudo-ginseng, phellinus igniarius, dandelion, gynostemma pentaphylla, liquorice and tea leaves, adding conventional auxiliary materials, and preparing the clinically acceptable preparation according to a conventional process.

7. The method of preparing the formulation of claim 6, comprising the steps of:

8. The method of preparing the formulation of claim 7, comprising the steps of:

9. A patch for treating malignant tumor is characterized in that the raw medicines of the patch comprise:

10. A method of making a patch according to claim 9 for the treatment of malignant tumors comprising the steps of: