CN107259293A - A kind of method that natural is prepared from clam worm - Google Patents

Abstract

The invention discloses a method for preparing natural antioxidants from clam worms. The clam worms are used as raw materials. After the clam worms are stored in a seawater damp environment, they are rinsed with deionized water, homogenized, ultrasonicated, soaked overnight, and then boiled until The clam worm skeleton is milky white, and after filtering to remove impurities, it is concentrated and freeze-dried to obtain a product. It adopts water-phase immersion extraction, decoction separation method, ultrafiltration membrane concentration and freeze-drying technology, without using other chemical reagents, simple operation process, good process stability and high extraction rate. The anti-oxidation product manufactured by the invention is stable in storage, has good anti-oxidation activity, and can obviously remove free radicals such as superoxide anions. Therefore, this product has good research and application prospects in medicine, health care and other aspects.

Description

A method for preparing natural antioxidant from clamworm

technical field

The invention belongs to the field of biological products and relates to a method for preparing natural antioxidants from clam worms.

Background technique

In taxonomy, clam worms belong to the phylum Annelida, polychaetes, wandering orders, and nereisidae, commonly known as sea worms, sea maggots, sea centipedes, and sea leeches. There are more than 80 kinds of clamworms in my country, and the economic species and varieties used for breeding mainly include: Japanese clamworm, spiny clamworm, double-tooth clamworm, etc. It likes to live in coastal beaches with fresh water inflow, and in the sand and mud of the middle intertidal zone to the subtidal zone. The larvae eat plankton, and the adults feed on humus. Lugworm has high nutritional value and is widely used in aquaculture. Nereid (Nereid), the English name—Clamworm, belongs to the phylum Annelids, a family of Polychaete Phyllophthora order, and has high economic value.

The clamworm body is long cylindrical, symmetrical on both sides, with a pointed rear end, and has many (more than 80 to 200) body segments. Can be divided into head, trunk and tail. The head is well developed and consists of two main parts, the preoral lobe and the perioral segment. The preoral lobe is a round triangular or conical fleshy lobe protruding in front of the mouth, with 2 pairs of simple round eyes, 1-2 protruding head tentacles and two large segmented tentacles on the ventral side of the front end. Perioral segment is a large annular segment, with a horizontally long mouth on the ventral surface, with 3 to 4 pairs of tentacles on both sides, a muscular snout that can protrude from the mouth, and a pair of chitinous mandibles at the front of the snout, the surface of the snout Smooth or chitinous indentations or soft papillae; the trunk has many segments with similar structure, and each segment has protruding fleshy flat protrusions on both sides, that is, verrucous feet. Verrucous feet are mostly bilobed with internal spines and external spines or sickle-shaped compound setae; the tail is the last segment or segments of the insect body, also known as anal segment, with a pair of anal palps, and the anus opens at the anal segment end back. The bristles have venomous glands, and the skin will be red, swollen and painful when pierced.

Nereis is rich in nutrition, not only is the bait organism that fish and shrimps eat, but also can be used as excellent bait. Coastal residents also regard lugworms with mature gonads as nutritional treasures. After drying, the boiled soup is as white as milk, with extremely delicious taste and high concentration, known as "natural monosodium glutamate". It is crispy and crispy after deep-fried, and it is a delicacy with wine. No matter domestically or exported, clamworms are very popular. In recent years, more researches on clamworm are some active ingredients extracted from it, such as bacteriostatic peptide, nereistin and so on. ,

In addition, because the clamworm has good environmental adaptability, some researchers regard it as an ecological key species to indicate the degree of environmental pollution.

The literature also reported a set of anti-oxidation system in the clam worm body. It was reported that the clam worm can prevent the poisoning of lead ions very well and has a good antioxidant function, but the article did not report how to prepare the antioxidant system into an anti-oxidation system. Methods and reports of oxidant preparations. The purpose of this invention is to provide a method for preparing natural antioxidants from clam worms, so as to make up for the shortcomings of existing reports.

Contents of the invention

The purpose of this invention is to provide a method for preparing natural antioxidants from clam worms. The prepared natural antioxidants have the effect of scavenging free radicals, and can be used for antioxidant protection of medicine and food, and can also be used as food additives and health products It is used to scavenge free radicals in the body and prevent body aging.

The present invention uses clamworm as the only raw material, such as japonica thorny clamworm, spiny clamworm, double-tooth clamworm, etc. The raw material is a natural marine organism, which is a food material with rich nutritional value, and does not need to add other toxic and side effects during the preparation process. Chemical reagents and additives, the preparation process is green and environmentally friendly, there is no environmental pollution, the product is green, degradable and absorbable, safe and has no side effects.

The raw materials used in the present invention are clam worms, such as Japanese thorn clam worms, spiny clam worms, double-tooth clam worms, etc., but are not limited to these three clam worm raw materials, and the preparation techniques of all clam worms in the present invention are identical.

The raw materials used in the present invention are fresh and healthy clam worms, which are treated in a humid seawater environment, temporarily raised in clean sea water, rinsed with deionized water, and surface impurities and mucus are removed, and then used in the preparation of clam worm natural antioxidants in the present invention .

In the present invention, the clam worm is used for 1-7 days for storage and treatment in a seawater damp environment, and for 1-3 days for temporary cultivation with seawater, rinses with deionized water, removes surface impurities and mucus, and quickly removes them for use. For the preparation of natural antioxidants of clamworm, it can also be stored at -20°C for later use.

In the present invention, the clam worm is first cut into segments, then homogenized with a tissue homogenizer, further ultrasonically crushed, and then deionized water is added to the system after crushing, left to soak for extraction, separated by heating, and filtered to obtain the first Crude extract and first extraction residue.

In the present invention, the clam worm is cut and then homogenized, the speed is 5000-10000 rpm, 10-15 min, and the ultrasonic crushing is further 10-20 min. After the crushing, the deionized water added to the system is 10-100 times the weight of the clam worm , the standing temperature is 4 ℃-45 ℃, the soaking extraction time is 8-20 h, and the heating separation condition is 60-90 min at boiling temperature.

In the present invention, add deionized water to the first extraction residue, let it stand for 1-5 hours, add deionized water 5-50 times the weight of clamworm, boil for 5-30 minutes, filter to obtain the second crude Extraction solution and second extraction residue.

In the present invention, the first crude extract and the second crude extract are combined, left standing at a low temperature of 4°C-10°C for 5-10 hours, filtered to remove incompatible substances, and the filtrate is collected, concentrated with an ultrafiltration membrane, freeze-dried, and sealed for storage. Obtain the clam worm natural antioxidant product of the present invention.

The invention adopts water-phase immersion extraction, decoction separation method, ultrafiltration membrane concentration and freeze-drying technology, does not use other chemical reagents, has simple operation process, good process stability and high extraction rate. The antioxidant product produced by the invention is stable in storage, has good antioxidant activity, and can obviously remove free radicals such as superoxide anions, so the product has good research and application prospects in medicine, health care and the like. And further illustrate the process of the present invention with the following specific examples.

detailed description

Nereist worms are stored in a damp environment in seawater, and after being temporarily raised in seawater, 1 portion of fresh clamworms is weighed and rinsed with deionized water to remove surface impurities and blood. Cut the clam worms into segments with scissors, homogenate, and sonicate. Soak 30 parts of deionized water at 37 ℃ overnight, boil the clam worm soaked overnight for 30 min, add 15 parts of deionized water after cooling and continue to boil until the skeleton of clam worm appears white, filter the mixture 3 times with double-layer gauze to remove completely Impurities, the collected filtrate was left to stand at low temperature for 1-5 hours, concentrated and then freeze-dried, and the obtained product was stored in a refrigerator at 4 °C.

The following several specific examples are taken as examples to further provide the manufacturing method of the present invention.

Example 1

The Nereis japonica was placed in a damp environment of seawater for 1 day, and the seawater was temporarily raised for 1 day. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 5,000 rpm for 15 minutes after shearing the clamworm individual, and ultrasonically break it for 10 minutes. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is brown viscous, and the yield is 13.63%.

Example 2

The clam worms were placed in a humid seawater environment for 7 days, and then temporarily raised in seawater for 3 days. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 10,000 rpm for 25 min and ultrasonically break for 20 min. Wash and soak 30 parts of deionized water overnight, decoct for 1 hour, add 15 parts of deionized water after cooling and continue to boil for 20 minutes, mix the two decoctions, cool and stand at 4 °C for 5 hours, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is reddish-brown viscous, and the yield is 10.80%.

Example 3

The clam worm was placed in the seawater damp environment for 3 days, and the seawater was temporarily raised for 2 days. Take 1 part of clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 5,000 rpm for 15 minutes after shearing the clamworm individual, and ultrasonically break it for 10 minutes. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is reddish-brown viscous, and the yield is 11.68%.

Example 4

Nereis japonica, Nereis spp., and Peris bidentata were respectively placed in a humid seawater environment for 3 days, and seawater was temporarily raised for 2 days. Take 1 part of the mixed clamworm, wash it with deionized water, and remove surface impurities and blood. Homogenize at 10,000 rpm for 20 min and ultrasonically break for 20 min. Wash and soak 30 parts of deionized water overnight, decoct for 1 h, add 15 parts of deionized water after cooling and continue to boil for 20 min, mix the two decoctions, cool and stand at 4 °C for 5 h, concentrate and freeze-dry. Promptly obtain the clam worm antioxidant product, the product is dark reddish brown viscous, and the yield is 19.66%.

Antioxidant Research

In the present invention, the scavenging rate of the product to superoxide anion and hydroxyl radical has been explored, and the result icon is as follows:

Figure 1. Scavenging of superoxide anion by Nereis worm extract. Figure 2. The scavenging of hydroxyl radicals by Nereis worm extract.

The experimental results prove that as the concentration of the material increases, its scavenging rate for superoxide anion and hydroxyl radicals increases, reaching 60% at 40 mg/ml, indicating that it has a significant antioxidant effect, so It has good research and application prospects in medicine and health care.

Claims (4)

1. the invention discloses a kind of method that natural is prepared from clam worm, it is characterized in that using clam worm as raw material, it is husky Silkworm is after the dark and damp environment storage processing of seawater, deionized water rinsing, homogenate, ultrasound, after soaked overnight, boils until clam worm bone Frame is presented after milkiness, filtering and impurity removing matter, and concentrated frozen is dried, and obtains product.

2. the manufacture method of the natural according to claim 1, it is characterized in that using clam worm as raw material, such as Japan Thorn clam worm, thorniness enclose clam worm, perinereis aibihitensis Grube etc., but be not limited to these three clam worm raw materials, and all clam worms are in the present invention Technology of preparation method is identical.

3. the manufacture method of the natural according to claim 1, it is characterized in that clam worm passes through dark and damp environmental treatment 1-7 days, using aqueous phase soak extraction, decoct separation method and obtain through refining standby polyphenoils.

4. the manufacture method of the natural according to claim 1, it is characterized in that 30 parts of deionized waters clean immersion After overnight, 1h is decocted, adds 15 parts of deionized water to continue to boil 20 min after cooling.