CN107231982A - Plant protection method and application - Google Patents
Plant protection method and application Download PDFInfo
- Publication number
- CN107231982A CN107231982A CN201710455653.2A CN201710455653A CN107231982A CN 107231982 A CN107231982 A CN 107231982A CN 201710455653 A CN201710455653 A CN 201710455653A CN 107231982 A CN107231982 A CN 107231982A
- Authority
- CN
- China
- Prior art keywords
- plant
- vegetable
- soil
- vaccine
- bacterium powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 239000002689 soil Substances 0.000 claims abstract description 64
- 241000894006 Bacteria Species 0.000 claims abstract description 51
- 229960005486 vaccine Drugs 0.000 claims abstract description 36
- 244000005700 microbiome Species 0.000 claims abstract description 29
- 239000003905 agrochemical Substances 0.000 claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 5
- 230000001681 protective effect Effects 0.000 claims abstract description 4
- 238000003306 harvesting Methods 0.000 claims abstract description 3
- 238000009331 sowing Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 110
- 201000010099 disease Diseases 0.000 claims description 36
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 36
- 235000013311 vegetables Nutrition 0.000 claims description 36
- 239000000843 powder Substances 0.000 claims description 34
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims description 24
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims description 23
- 229930014456 matrine Natural products 0.000 claims description 23
- 229920001542 oligosaccharide Polymers 0.000 claims description 19
- 150000002482 oligosaccharides Chemical class 0.000 claims description 19
- 241000726221 Gemma Species 0.000 claims description 17
- 108091006088 activator proteins Proteins 0.000 claims description 14
- 239000007921 spray Substances 0.000 claims description 13
- 241000607479 Yersinia pestis Species 0.000 claims description 12
- 238000007598 dipping method Methods 0.000 claims description 12
- 238000004080 punching Methods 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 4
- 239000002917 insecticide Substances 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 241000194108 Bacillus licheniformis Species 0.000 claims 1
- 241000219112 Cucumis Species 0.000 claims 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims 1
- 235000021384 green leafy vegetables Nutrition 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 10
- 239000000126 substance Substances 0.000 abstract description 10
- 239000000575 pesticide Substances 0.000 abstract description 9
- 230000008635 plant growth Effects 0.000 abstract description 5
- 241000721047 Danaus plexippus Species 0.000 abstract description 2
- 238000012271 agricultural production Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 description 26
- 238000010790 dilution Methods 0.000 description 26
- 244000131316 Panax pseudoginseng Species 0.000 description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 18
- 230000000694 effects Effects 0.000 description 13
- 239000008139 complexing agent Substances 0.000 description 11
- 239000006041 probiotic Substances 0.000 description 10
- 235000018291 probiotics Nutrition 0.000 description 10
- 238000002791 soaking Methods 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 7
- 240000003768 Solanum lycopersicum Species 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 6
- 241000180649 Panax notoginseng Species 0.000 description 6
- 235000003143 Panax notoginseng Nutrition 0.000 description 6
- 241000700605 Viruses Species 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 239000005416 organic matter Substances 0.000 description 6
- 208000035240 Disease Resistance Diseases 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 150000001768 cations Chemical class 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000002708 enhancing effect Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000033228 biological regulation Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 229940023143 protein vaccine Drugs 0.000 description 4
- 241001124076 Aphididae Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 206010027146 Melanoderma Diseases 0.000 description 3
- 208000031888 Mycoses Diseases 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 241000918585 Pythium aphanidermatum Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000014509 gene expression Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000000411 inducer Substances 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000002686 phosphate fertilizer Substances 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000228197 Aspergillus flavus Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 244000302544 Luffa aegyptiaca Species 0.000 description 2
- 235000009814 Luffa aegyptiaca Nutrition 0.000 description 2
- 241001330975 Magnaporthe oryzae Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 244000302512 Momordica charantia Species 0.000 description 2
- 235000009811 Momordica charantia Nutrition 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000228150 Penicillium chrysogenum Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241000255969 Pieris brassicae Species 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 2
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 2
- 241001249129 Scirpophaga incertulas Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 208000030961 allergic reaction Diseases 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000005784 autoimmunity Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 238000007596 consolidation process Methods 0.000 description 2
- 230000004665 defense response Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- -1 phosphate anion Chemical class 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 229940031937 polysaccharide vaccine Drugs 0.000 description 2
- 229940072033 potash Drugs 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
- 235000015320 potassium carbonate Nutrition 0.000 description 2
- 229910001414 potassium ion Inorganic materials 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- ZSBXGIUJOOQZMP-BHPKHCPMSA-N sophoridine Chemical compound C1CC[C@@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-BHPKHCPMSA-N 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- XVPBINOPNYFXID-JARXUMMXSA-N 85u4c366qs Chemical compound C([C@@H]1CCC[N@+]2(CCC[C@H]3[C@@H]21)[O-])N1[C@@H]3CCCC1=O XVPBINOPNYFXID-JARXUMMXSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000405758 Betapartitivirus Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 108090000565 Capsid Proteins Proteins 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102100023321 Ceruloplasmin Human genes 0.000 description 1
- 241000258920 Chilopoda Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 1
- 208000021661 Elimination disease Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 101000668058 Infectious salmon anemia virus (isolate Atlantic salmon/Norway/810/9/99) RNA-directed RNA polymerase catalytic subunit Proteins 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 1
- 235000018365 Momordica dioica Nutrition 0.000 description 1
- 108700001237 Nucleic Acid-Based Vaccines Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 235000002789 Panax ginseng Nutrition 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000239226 Scorpiones Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- AAGFPTSOPGCENQ-UHFFFAOYSA-N Sophocarpin I Natural products C1CCC2CN3C(=O)C=CCC3C3C2N1CCC3 AAGFPTSOPGCENQ-UHFFFAOYSA-N 0.000 description 1
- IGXQFUGORDJEST-UHFFFAOYSA-N Sophocarpine Natural products O=C1C=CCC2C3CCCC4CCCC(CN12)C34 IGXQFUGORDJEST-UHFFFAOYSA-N 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 241000271897 Viperidae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 230000003288 anthiarrhythmic effect Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 229940031567 attenuated vaccine Drugs 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 229960001212 bacterial vaccine Drugs 0.000 description 1
- 239000010231 banlangen Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000007799 cork Substances 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000010429 evolutionary process Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000020764 fibrinolysis Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- QMGGMESMCJCABO-UHFFFAOYSA-N n-oxysophocarpine Chemical compound C12C3CCC[N+]2([O-])CCCC1CN1C3CC=CC1=O QMGGMESMCJCABO-UHFFFAOYSA-N 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229940023146 nucleic acid vaccine Drugs 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229930015582 oxymatrine Natural products 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000018528 secretion by tissue Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- AAGFPTSOPGCENQ-JLNYLFASSA-N sophocarpine Chemical compound C1CC[C@H]2CN3C(=O)C=CC[C@@H]3[C@@H]3[C@H]2N1CCC3 AAGFPTSOPGCENQ-JLNYLFASSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
Landscapes
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of plant protection method and application, this method includes improving planting soil with microorganism live bacteria, carries out the total process protective from sowing to harvesting to plant by plant vaccine and plant-derived agricultural chemicals.It this method solve application unicity of the domestic biotechnology in plant protection at present; the problem of product of each biological field is independently used; by various biotechnology compatibility applications; between various biotechnologys each other monarch, minister, help, performance is acted synergistically, form complete bio technical plant protection system；Play few with most of chemical fertilizer, reduce most of chemical pesticide, the micro-ecological environment for improving soil, improving plant growth, improve agricultural product quality, volume increase；So as to improve agricultural production supply, increase economic efficiency.This method is simple and easy to apply to be grasped, it is established that a complete plant protection system based on biotechnology.
Description
Technical field
The present invention relates to technical field of life science, plant protection is carried out more particularly, to a kind of integrated use biotechnology
Method and application.
Background technology
Plant Protection is related to the science such as botany, zoology, microbiology, agroecology, information science, research
The regularity of occurrence and development of harmful organism and the technology for proposing the comprehensive regulation.
Pseudo-ginseng also known as pseudo-ginseng, the famous pharmacy man Li Shizhen (1518-1593 A.D.) of the Ming Dynasty are called " invaluable ".Pseudo-ginseng is one in Chinese medicine
Jewel, Qing Dynasty's pharmaceutical texts《A Supplement to the Compendium of Materia Medica》Described in：" ginseng qi-tonifying first, pseudo-ginseng enriches blood first, taste with and work(also
Deng, therefore claim Panax pseudoginseng, it is the most precious person in Chinese medicine.Modern pharmacological research shows that pseudo-ginseng has coronary artery dilator flow, anxious
Property myocardial ischemia, anti-arrhythmia is anti-oxidant, anti-aging, suppresses platelet function and promotes fibrinolysis activity.Pseudo-ginseng is to enhancing people
Body immunity, blood-supplementing blood-nourishing, anti-aging and antifatigue, the antitumor effect having had, to cardiovascular and cerebrovascular disease systemic control
There is remarkable efficacy.It can be seen that, the production and processing of pseudo-ginseng are the significant things related to people's health care.
Pseudo-ginseng directly as food or can be processed into various medicines, the use of agricultural chemicals and fertilizer as medical and edible dual purpose plant
It must be carried out in strict accordance with national standard, so, the preventing and controlling for the pest and disease damage in notoginseng planting are just particularly important.
Likewise, the daily required vegetables of people also will avoid using agricultural chemicals as far as possible, so, equally deposited for various vegetables
Problem is prevented and treated in pest and disease damage.
And current biotechnology is mostly relatively simple for the protection application of plant, do not set up one it is complete with
Plant protection system based on biotechnology.
The content of the invention
It is an object of the invention to provide a kind of plant protection method, set up one it is complete based on biotechnology
Plant protection system.
To achieve the above object, technical scheme is as follows：
A kind of plant protection method, this method includes improving planting soil with microorganism live bacteria, passes through plant vaccine
The total process protective from sowing to harvesting is carried out to plant with plant-derived agricultural chemicals.
Preferably, it is described to improve planting soil with microorganism live bacteria and be included in the soil of planting plants and apply micro- life
Thing viable bacteria.
Preferably, the microorganism live bacteria includes gemma bacterium powder, lactic acid bacteria powder, many slime mould powder, wax-like bacillus powder or ground
Clothing bacillus powder
Preferably, the plant vaccine includes oligosaccharide kind vaccine or activator protein class vaccine.
Preferably, the plant-derived agricultural chemicals is the agricultural chemicals containing matrine.
Application of the upper plant protection method in notoginseng planting.
Preferably, gemma bacterium powder is applied in the soil of plantation pseudo-ginseng, the spore content of the gemma bacterium powder is 2*
1010CFU/g；Before pseudo-ginseng is colonized, every mu of soil inoculation gemma bacterium powder 500-1000g；During Panax notoginseng Growth, bud will be contained
The liquid of spore bacterium powder is by pouring root, root dipping, and punching is applied acts on pseudo-ginseng plant with the mode of foliage-spray；
Panax notoginseng seeds are dressed seed using plant vaccine, soaked seed, pouring root, the behaviour of foliage-spray are carried out to pseudo-ginseng plant
Make；
The prevention and control of plant diseases, pest control is carried out to pseudo-ginseng plant using the insecticide containing matrine.
Application of the above-mentioned plant protection method in vegetable cultivation.
Preferably, gemma bacterium powder is applied in the soil of planting vegetable, the spore content of the gemma bacterium powder is 2*
1010CFU/g；Before vegetable permanent planting, every mu of soil inoculation gemma bacterium powder 500-1000g；During vegetable growth, bud will be contained
The liquid of spore bacterium powder is by pouring root, root dipping, and punching is applied acts on vegetable plant strain with the mode of foliage-spray；
Vegetable seeds is dressed seed using plant vaccine, soaked seed, pouring root, the behaviour of foliage-spray are carried out to vegetable plant strain
Make；
The prevention and control of plant diseases, pest control is carried out to vegetable plant strain using the insecticide containing matrine.
Beneficial effects of the present invention are as follows：
Country's biotechnology is relatively simple in the application of plant protection at present, and the product of each biological field is independently operated, this
Inventive method is by various biotechnology compatibility applications, it is established that a complete plant protection based on biotechnology
System.
Embodiment
In order to illustrate more clearly of the present invention, with reference to preferred embodiment, the present invention is described further.Ability
Field technique personnel should be appreciated that following specifically described content is illustrative and be not restrictive, and this should not be limited with this
The protection domain of invention.Unreceipted actual conditions person in embodiment, the condition advised according to normal condition or manufacturer is carried out.Institute
It is the conventional products that can be obtained by commercially available purchase with reagent or the unreceipted production firm person of instrument.
When biotechnology is used for plant protection by the country at present, application mode is relatively simple, and the present inventor is by
Monarch, minister are cured, helps, prescription theory is applied among plant protection, plant protection is treated as an organism, each biological skill
Art itself is bioactive substance, is the similar or identical organic prescription of one group of structure, each biotechnology compatibility is used, and is produced
The synergy of plant protection, it is established that a complete system that plant protection is carried out based on biotechnology.
The biotechnology that the inventive method is related to includes the use of microorganism live bacteria, the use of plant vaccine and plant source
The use of property biological pesticide, according to the individual instances of different plants, three kinds of above-mentioned biotechnologys of integrated use, realize one it is complete
Whole plant protection system.
Soil is irrigated, using chemical fertilizer and agricultural chemicals due to unreasonable, causes that soil nutrient is unbalance, organic matter declines, soil plate
Knot is serious, causes medicinal material, fruit tree, flowers, and the disease such as vegetables increases, and seriously injures the healthy of people.Therefore, protecting field
Soil improvement turns into a kind of inevitable choice of sustainable agriculture development.
The crumb structure of soil is the important indicator of soil fertility, and the destruction of soil granular structure causes soil water-retaining, guarantor
Fertile ability and permeability reduction, cause soil hardening.
The content of organic matter is an important indicator of soil fertility and crumb structure, and the reduction of organic matter causes soil
It is hardened.The soil organism is the important component of soil granular structure.The decomposition of the soil organism is the activity with microorganism
Come what is realized.Excessively applied into soil after nitrogenous fertilizer, the Nitrogen supplying of microorganism increases by 1 part, and the carbon accordingly consumed is increased by
25 parts, the carbon consumed derives from the soil organism, and the content of organic matter is low, the activity of microorganism is influenceed, so as to influence soil
The formation of crumb structure, causes soil hardening.
Soil granular structure is that electronegative soil clay particle and organic matter are formed by the polyvalent cation connection of positively charged
's.Soil particle is connected into bulky grain formation soil granular structure by polyvalent cation in key bridge form.Cation in soil
Based on the calcium of divalent, magnesium ion, when excessively applying phosphate fertilizer into soil, calcium, magnesium etc. in the phosphate anion and soil in phosphate fertilizer
Cation combines to form insoluble phosphate, that is, wastes phosphate fertilizer, soil granular structure is destroyed again, cause soil hardening.
When excessively applying potash fertilizer into soil, the potassium ion displacement property in potash fertilizer is especially strong, can will form soil granular knot
The polyvalent cation of structure is cemented out, and the potassium ion of monovalence is acted on without key bridge, and the key bridge of soil granular structure is destroyed
, crumb structure is also just destroyed, soil hardening is caused.
Solving the method for soil hardening --- selection applies microorganism live bacteria and nurses one's health soil.It is nontoxic to crop, have no side effect,
Can break soil hardening, chesson, improve the gas permeability of soil, promote soil microbial activities, enhancing soil rich water penetration,
Pest and disease damage is reduced, so as to reduce the consumption of agricultural chemicals or microbial manure is applied into soil, the secretion of microorganism can be molten
The phosphate in soil is solved, phosphorus element is discharged, meanwhile, also potassium and micro- cation release are come out, with key bridge shape
Formula recovers crumb structure, eliminates soil hardening.Improved soil is incremental process, can by apply the organic fertilizer that become thoroughly decomposed come
Slowly realize.
Microorganism can produce substantial amounts of gas during fermentation, and the gas that active strong microbial inoculum is produced is more,
These bubbles make soil become soft.
In microbial-bacterial fertilizer contain substantial amounts of beneficial bacterium, after bacterial manure is manured into soil, beneficial microbe rapidly in soil with
Geometric progression procreation diffusion, and functional microorganism metabolite, can improve the physicochemical character of soil, improve hydrophily, inhale
Attached property, enhancing permeability and retain water and nutrients ability, microbial-bacterial fertilizer not only contain a large amount of fixed nitrogen, phosphorus decomposing, potassium decomposing active bacteria, also contained
There are humic acid, organic matter and various trace elements, so as to make farming increasing both production and income, and be improved the effect of quality.
Matrine is extracted and is made through organic solvents such as ethanol by the dry root of legume kuh-seng, plant, fruit, is
Alkaloid.Generally Banlangen, its main component has a variety of biologies such as matrine, sophocarpine, N-Oxysophocarpine, Sophoridine
Alkali, with matrine, oxymatrine concentration highest.
Matrine is a kind of botanical pesticide, the characteristics of with specificity, natural sex, only biological produces work to specific
With, can be decomposed rapidly in the Nature, final product be carbon dioxide and water.Secondly matrine is active to harmful organism
Plant endogenous chemical substance, composition is not single, but not close many of the close multigroup and chemical constitution of chemical results
The combination of group, complements each other, plays a role jointly.3rd, matrine make it be difficult to lead because a variety of chemical substance collective effects
Cause nuisance to develop immunity to drugs, can be used for a long time.4th, corresponding insect directly will not be killed completely, but control insect
Biotic population quantity will not badly influence the production and procreation of the plant population.Matrine and general high poison, the change of high residue
Learn to farm medicine obvious differences, be very green, environmentally friendly.
In recent years, with the development of scientific research, it was demonstrated that plant has immune resistance, and this immune resistance can be by nature
A variety of in boundary lure the anti-factor or elicitor to induce or activate.Plant is reform of nature circle environment in long-term evolutionary process
Change and resist the invasion of pathogenic microorganism and form the functional mass of various autoimmune, the autoimmunity resistance of plant can
It is divided into physics to be immunized and chemo-immunity.The immune cuticula for referring mainly to the formation of the positions such as blade face of physics, wax coat, wounded tissue week
The cork tissue to be formed is enclosed, the height lignifying of plant tissue, the various trees slurry and resin of tissue secretion etc. defends the micro- life of cause of disease
The invasive ability of thing；Chemo-immunity is primarily referred to as secondary substance that plant formed in itself to the killing of some pathogens or suppresses to make
With.
The essence of vaccine is that a certain antigen component is acted on into organism, and excitating organism is produced to the carrier of the antigen or antigen
Raw immune response, so as to protect body infecting from cause of disease, the carrier format of this antigen or antigen is to claim vaccine, vaccine
It was found that the development to human history is significant, threatens the elimination of the variola virus of the mankind to open the mankind and fought using vaccine
Win new era of disease, make people more believe firmly vaccine to control and the effect of elimination disease.In broad terms, plant virus epidemic disease
Seedling not only includes virus and its component, in addition to the similar animal vaccine of those modes of action, inducible plant strengthen antiviral energy
The material of power.
Plant disease partitivirus venereal disease evil, fungal disease and bacterial disease.Plant disease occurs in various plants, its
Route of transmission is similar, specific.Plant virus vaccine include attenuated vaccine, bacterial vaccine, protein vaccine, nucleic acid vaccine,
Polysaccharide vaccine, small molecule vaccine.Wherein, protein vaccine includes activator protein vaccine and virus capsid protein vaccine, activator protein
It is from a variety of fungies such as Alternariaspp, sheath blight fungus, Aspergillus flavus, botrytis, rice blast fungus, Penicillium notatum, Trichoderma, sickle-like bacteria
Middle screening, the class novel protein isolated and purified out, the albumen are improved and planted mainly by molecular immune system in activated plant body
Thing autoimmunity, by exciting a series of metabolic regulations in plant, promotes plant roots cauline leaf to grow and improve chlorophyll
Content, so as to reach the purpose improved as yield.Polysaccharide vaccine is grabbed including oligosaccharide, and oligosaccharide is one in nature
Class has the complex carbohydrates of biological regulation function, and some oligosaccharides can strengthen the ability of plant disease-resistant pathogenic microorganism,
One of effective vegetative practice will be turned into by plant is obtained systemic disease resistance using it as inducer.
It is at present, domestic that by plant immune inducible protein exciton anti-disease vaccines, referred to as albumen lures anti-in agricultural chemicals classification
Agent, albumen inducer is that a class can excite the distinctive signal albumen of plant defense response gene expression and allergic reaction, its energy
By exciting the disease-resistant functional gene of plant itself to express, strengthen the immunocompetence of plant for disease, promote plant growth.Albumen
The disease resistance mechanisms of the mechanism of action of inducer similar animal immune in nature, belong to a kind of new, spectrum, efficient, multi-functional
Biological pesticide.Over nearly 20 years, found from pathogenic strain a variety of with induction plant spectral resistance and somatotrophic sharp
Send out subclass albumen.Mainly include allergen protein, hidden ground albumen and activator protein.Exciton can cause the induction of plant disease resistance genes
Expression, the generation of disease-resistance substance, Apoptosis and allergic reaction etc., these reactions prevent or limited generation and the hair of disease
Exhibition, mitigates the extent of injury to plant.
Activator protein is sieved from the pathogen of Botrytis cinerea, Alternariaspp, Aspergillus flavus, rice blast fungus, Penicillium notatum, sheath blight fungus
The albuminoid exciton select, separate, being purified into.The mechanism of action shows that activator protein induces the signal transduction of plant, causes plant
A series of related enzyme activity increases and the enhancing of gene expression amount, excite a series of metabolic regulations in plant, promote in object
The growth of plant roots cauline leaf, raising chlorophyll content, enhancing plant improve crop yield to the resistance of pest and disease damage.
In recent years, it is found that most protein surfaces are all connected with the oligonucleotide chain that number is not waited, these oligosaccharides in organism
All there is important function during many vital movements, it has been found that the oligosaccharides not only combined with protein has extensive
Biological effect, free oligosaccharides also has important biological effect in many life processes, and some oligosaccharides can conduct
A kind of signaling molecule regulates and controls the defense response grown with plant resistant adverse circumstance of plant.The species of oligosaccharides plant vaccine：β-
The sugar of Portugal seven, the oligosaccharides obtained by chitin and chitosan, oligogalacturonans, wooden poly oligosaccharide, lipopolysaccharides, peptide oligosaccharides.
The plant acquired immunity that plant vaccine is excited has that disease-resistant spectrum is wide, the duration is longer and induction or exciton kind
The features such as class is more, oligosaccharide is a class signaling molecule of international latest find, with regulating plant growth, development, diseases prevention and anti-
Function in terms of disease, can induce the immune system resistance and regulating plant growth of plant, in particular according to separate sources
Oligosaccharide is to the action character of different pathogens, so as to develop the baptism oligosaccharide agricultural chemicals of all kinds of diseases, therefore such
Product cost is low, and drug effect is high, and the five poisonous creatures: scorpion, viper, centipede, house lizard, toad are nuisanceless, therefore the either manufacturing enterprise of oligosaccharides biological pesticide, or proportion of crop planting
Therefore person will obtain huge economic benefit.
Microorganism live bacteria improved soil is used, strengthens the disease resistance of plant using plant vaccine, compatibility uses plant-derived agriculture
Medicine, collaboration plays a protective role to every kind of plant.
Microorganism live bacteria include microbial-bacterial fertilizer, microbial bacterial agent, microbial bacterial agent include single microbial inoculum, composite bacteria agent and
Bacterium powder, microorganism live bacteria can eliminate soil-borne disease, prevent and treat sprout term disease, prevent dead root.
Microorganism probiotic complexing agent in the application is the complexing agent of " gold medal " brand；Plant vaccine is the Chinese Academy of Agricultural Sciences
The plant vaccine that Institute of Plant Protection Langfang agricultural chemicals pilot plant is produced.
Embodiment 1
By microorganism live bacteria, the protection system to pseudo-ginseng that plant vaccine and plant-derived agricultural chemicals are built, the master of pseudo-ginseng
Disease is wanted to include black spot, Northern leaf spot, damping-off, powdery mildew, gray mold and aphid etc..
1. the use of microorganism live bacteria
1) the consolidation phase of notoginseng planting, Soil K+adsorption is carried out to plot, according to testing result, selection spore content 2*
1010CFU/g microorganism live bacteria gemma bacterium powder.
Application method：1 kilogram of every mu of bacterium powder, which stirs, is spread fertilizer over the fields, or farm manure is applied together；
2) before pseudo-ginseng field planting, microorganism probiotic complexing agent is diluted with 500-1000 times of clear water；
3) pouring root, root dipping are carried out during Panax notoginseng Growth with the dilution of probiotics complexing agent, punching is applied and industry face is sprayed；
Pouring root, root dipping, probiotics complexing agent is used with 300---600 times of clear water dilution；
Operation, every mu of use 1 are applied in punching --- 2 kilograms of gemma bacterium powder；
Foliage-spray, 300 are used by probiotics complexing agent --- 600 times of clear water dilution, in reproductive stage every ten days
Spray once, be used in conjunction 2 times, volume increase 10% --- 30%, and can significantly modify quality；
2. plant vaccine application method
Plant vaccine, which is mainly amino-oligosacchride or the wettable powder of toVerticilliumdahliaActivitie Activitie S of Relative activator protein, can prevent and treat pseudo-ginseng plant
Fungus disease, bacterium class disease, virus type disease, the black spot of such as pseudo-ginseng, Northern leaf spot, damping-off, root rot, white powder,
Gray mold；
Application method：Oligosaccharide kind, activator protein class plant vaccine, can be used alone, also can compatibility use；
1. with planting, seed dressing operation is carried out with the aqueous solution of every liter of 5-20 grams of Activating protein-1 containing toVerticilliumdahliaActivitie Activitie S of Relative；
2. seed soaking, root dipping or transplanting, 600 are used by amino-oligosacchride --- 800 times of clear water dilution, dilution is used to soaking seed,
Root dipping or transplanting；
3. pouring root, 600 are used by amino-oligosacchride --- and 800 times of clear water dilution, dilution is used for pouring root；
4. industry face is sprayed, and amino-oligosacchride is used into 600 --- and 800 times of clear water dilution, dilution is sprayed for industry face；From three
Seven seedlings are unearthed to be sprayed once every 7 days, was used in conjunction three times, can prevent the most of disease of pseudo-ginseng；
5. more than 2 years pseudo-ginseng seedlings are in annual rainy season, and Yunnan Province is 4 --- in June, toVerticilliumdahliaActivitie Activitie S of Relative activator protein is used
600 --- 1000 times of clear water dilution, dilution is used to spray, was sprayed once every 7 days, be used in conjunction three times, pseudo-ginseng can be prevented big
Some Disease；
3. plant-derived biological pesticide
Contain matrine in agricultural chemicals, the characteristics of matrine has specificity, natural sex is decomposed rapidly, most in the Nature
End-product is carbon dioxide and water, and matrine has insecticidal activity, bactericidal activity, effectively reduces Pesticide use；
Pest control object：Aphid, cabbage caterpillar, yellow rice borer rice leaf the moon, the white pretty young woman in the U.S. etc.；
Application method：Insect pest aqueous matrine solution when in the initial stage of that insect population is low, 1000 are used by matrine --- and 2000 times of clear water are dilute
Release, spraying operation is carried out with dilution；
The larval phase increase dosage of insect pest serious phase, is sprayed once, continuously using secondary for every seven days.
Embodiment 2
By microorganism live bacteria, the protection system to vegetables that plant vaccine and plant-derived agricultural chemicals are built.
Vegetables in the present embodiment are tomato.
1. the use of microorganism live bacteria
Microorganism live bacteria can eliminate soil-borne disease, prevent and treat sprout term disease, the anti-dead root of plant.
1) the consolidation phase of tomato planting, Soil K+adsorption is carried out to plot, according to testing result, selection spore content 2*
1010CFU/g microorganism live bacteria gemma bacterium powder；
Application method：1 kilogram of every mu of bacterium powder, which stirs, is spread fertilizer over the fields, or farm manure is applied together；
2) before tomato field planting, microorganism probiotic complexing agent is diluted with 500-1000 times of clear water, dilution is then used
Seed soaking；
3) during tomato growth, pouring root, root dipping are carried out with the dilution of probiotics complexing agent, punching is applied and industry face is sprayed；
Pouring root, root dipping, probiotics complexing agent is diluted with 300 times of clear water, and dilution carries out pouring root or root dipping operation；
Punching is applied, every mu of use 1 --- 300 times of dilutions of 2 kilograms of probiotics complexing agent；
Foliage-spray, 300 are used by probiotics complexing agent --- and 600 times of clear water dilution, dilution is every in reproductive stage
Sprayed once, be used in conjunction 2 times every ten days, volume increase 10% --- 30%, and can significantly modify quality；
2. plant vaccine application method
Plant vaccine is mainly the wettable powder of amino-oligosacchride or toVerticilliumdahliaActivitie Activitie S of Relative activator protein, can prevent and treat tomato plants
Fungus disease, bacterium class disease, virus type disease, the black spot of such as tomato, Northern leaf spot, damping-off, root rot, white powder,
Gray mold；
Application method：Oligosaccharide kind, activator protein class plant vaccine can be used alone, also can compatibility use；
1. with planting, every liter carries out seed dressing operation with the aqueous solution of 0.8-1.0 milligrams of activator protein containing toVerticilliumdahliaActivitie Activitie S of Relative；
2. soak seed, amino-oligosacchride is diluted with 600-800 times of clear water, dilution temperature is 30 DEG C, to the kind of tomato
Son, soaks seed 6-8 hours；
When 3. transplanting, root dipping operation is carried out with 600-800 times of dilution of amino-oligosacchride；
4., can with the dilution or toVerticilliumdahliaActivitie Activitie S of Relative activator protein of 600-800 times of amino-oligosacchride after leaf is unearthed or transplanted
The clear water dilution of 1000-2000 times of WP carries out spraying operation to blade face, and every 7 days once, continuous to use three times；
3. plant-derived biological pesticide
Agricultural chemicals selects aqueous matrine solution；
The characteristics of matrine has specificity, natural sex, decomposes rapidly in the Nature, final product be carbon dioxide and
Water, matrine has insecticidal activity, bactericidal activity, effectively reduces Pesticide use；
Pest control object：Aphid, cabbage caterpillar, yellow rice borer rice leaf the moon, the white pretty young woman in the U.S. etc.；
Application method：Insect population lowstand, 1000 are used by matrine --- and 2000 times of clear water dilution, dilution is used to spray behaviour
Make；
Insect density is big, 2-3 age of larva when, sprayed with 600-800 times of aqueous matrine solution；
Embodiment 3
It is same as Example 2, the difference is that the vegetables of protection are capsicum.
Embodiment 4
It is same as Example 2, the difference is that the vegetables of protection are eggplant.
Embodiment 5
It is same as Example 2, the difference is that the vegetables of protection are muskmelon.
Embodiment 6
It is same as Example 2, the difference is that the vegetables of protection are watermelon, and in seed soaking step, during the seed soaking of watermelon seed
Between be 12-24 hours.
Embodiment 7
It is same as Example 2, the difference is that the vegetables of protection are sponge gourd, and in seed soaking step, during the seed soaking of sponge gourd seed
Between be 2-3 days, and often change within 8-10 hours a dilution.
Embodiment 8
It is same as Example 2, the difference is that the vegetables of protection are balsam pear, and in seed soaking step, during the seed soaking of seed of bitter gourd
Between be 2-3 days, and often change within 8-10 hours a dilution.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of plant protection method, it is characterised in that this method includes improving planting soil with microorganism live bacteria, passes through
Plant vaccine and plant-derived agricultural chemicals carry out the total process protective from sowing to harvesting to plant.
2. according to the method described in claim 1, it is characterised in that the microorganism live bacteria, which improves planting soil, to be included
Apply microorganism live bacteria in the soil of planting plants.
3. method according to claim 2, it is characterised in that the microorganism live bacteria includes gemma bacterium powder, lactic acid bacteria powder,
Many slime mould powder, wax-like bacillus powder or bacillus licheniformis powder.
4. according to the method described in claim 1, it is characterised in that the plant vaccine includes oligosaccharide kind vaccine or activator protein
Class vaccine.
5. according to the method described in claim 1, it is characterised in that the plant-derived agricultural chemicals is the agricultural chemicals containing matrine.
6. application of the plant protection method as claimed in claim 1 in vegetable cultivation.
7. application according to claim 6, it is characterised in that apply gemma bacterium powder in the soil of planting vegetable, described
The spore content of gemma bacterium powder is 2*1010CFU/g；Before vegetable permanent planting, every mu of soil inoculation gemma bacterium powder 500-1000g；
During vegetable growth, by the liquid containing gemma bacterium powder by pouring root, root dipping, punching is applied acts on vegetable with the mode of foliage-spray
Dish plant；
Vegetable seeds is dressed seed using plant vaccine, soaked seed, pouring root, the operation of foliage-spray are carried out to vegetable plant strain；
The prevention and control of plant diseases, pest control is carried out to vegetable plant strain using the insecticide containing matrine.
8. application of the plant protection method as claimed in claim 1 in vegetable cultivation.
9. application according to claim 8, it is characterised in that
Apply gemma bacterium powder in the soil of planting vegetable, the spore content of the gemma bacterium powder is 2*1010CFU/g；In vegetables
Before field planting, every mu of soil inoculation gemma bacterium powder 500-1000g；During vegetable growth, the liquid containing gemma bacterium powder is led to
Cross pouring root, root dipping, punching is applied acts on vegetable plant strain with the mode of foliage-spray；
Vegetable seeds is dressed seed using plant vaccine, soaked seed, pouring root, the operation of foliage-spray are carried out to vegetable plant strain；
The prevention and control of plant diseases, pest control is carried out to vegetable plant strain using the insecticide containing matrine.
10. application according to claim 8 or claim 9, it is characterised in that the vegetables include leaf vegetables or melon dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710455653.2A CN107231982A (en) | 2017-06-16 | 2017-06-16 | Plant protection method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710455653.2A CN107231982A (en) | 2017-06-16 | 2017-06-16 | Plant protection method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107231982A true CN107231982A (en) | 2017-10-10 |
Family
ID=59987636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710455653.2A Pending CN107231982A (en) | 2017-06-16 | 2017-06-16 | Plant protection method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107231982A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168804A (en) * | 2018-08-31 | 2019-01-11 | 云南五佳生物科技有限公司 | A kind of comprehensive prevention method of flue-cured tobacco common disease and pest |
| CN109452065A (en) * | 2018-11-01 | 2019-03-12 | 青岛市农业科学研究院 | A kind of organic tea fertilizing and planting method |
| CN109906903A (en) * | 2019-04-25 | 2019-06-21 | 盐城市新洋农业试验站(江苏沿海地区农业科学研究所新洋试验站) | Perennial radix cynanchi bungei plants field processing method |
| CN113940241A (en) * | 2021-10-29 | 2022-01-18 | 山西省药品审评中心 | Cultivation method for ecological control of radix bupleuri |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391876A (en) * | 2011-09-29 | 2012-03-28 | 山东靠山生物科技有限公司 | Composite biological soil modifier and application thereof |
| CN104782365A (en) * | 2015-04-10 | 2015-07-22 | 韦永梁 | Cowpea planting pest and disease damage green prevention and control method |
| CN105154364A (en) * | 2015-09-21 | 2015-12-16 | 中绿三品生态科技(北京)有限公司 | Probiotics used for agricultural planting and preparing and using method thereof |
| CN106489514A (en) * | 2016-12-19 | 2017-03-15 | 重庆信首科技有限公司 | A kind of insect prevention implantation methods of booth vegetable |
-
2017
- 2017-06-16 CN CN201710455653.2A patent/CN107231982A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391876A (en) * | 2011-09-29 | 2012-03-28 | 山东靠山生物科技有限公司 | Composite biological soil modifier and application thereof |
| CN104782365A (en) * | 2015-04-10 | 2015-07-22 | 韦永梁 | Cowpea planting pest and disease damage green prevention and control method |
| CN105154364A (en) * | 2015-09-21 | 2015-12-16 | 中绿三品生态科技(北京)有限公司 | Probiotics used for agricultural planting and preparing and using method thereof |
| CN106489514A (en) * | 2016-12-19 | 2017-03-15 | 重庆信首科技有限公司 | A kind of insect prevention implantation methods of booth vegetable |
Non-Patent Citations (3)
| Title |
|---|
| 丁安伟，王振月: "《中药资源综合利用与产品开发》", 30 April 2013 * |
| 常庆: "《白色农业 三色农业与原生态农业》", 31 January 2007 * |
| 科学技术部农村科技司: "《生物技术在种植业中的应用与实践》", 31 December 2015 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168804A (en) * | 2018-08-31 | 2019-01-11 | 云南五佳生物科技有限公司 | A kind of comprehensive prevention method of flue-cured tobacco common disease and pest |
| CN109452065A (en) * | 2018-11-01 | 2019-03-12 | 青岛市农业科学研究院 | A kind of organic tea fertilizing and planting method |
| CN109906903A (en) * | 2019-04-25 | 2019-06-21 | 盐城市新洋农业试验站(江苏沿海地区农业科学研究所新洋试验站) | Perennial radix cynanchi bungei plants field processing method |
| CN113940241A (en) * | 2021-10-29 | 2022-01-18 | 山西省药品审评中心 | Cultivation method for ecological control of radix bupleuri |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103964973B (en) | The special seed dressing of peanut | |
| CN103300069A (en) | Biological bactericide composition and application thereof to wilt | |
| CN107667737A (en) | A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage | |
| BG67257B1 (en) | Bacterial strain of bacillus amyloliquefaciens subsp. plantarum bs89 as a means of increasing plant productivity and their protection against diseases | |
| CN103283725A (en) | Sterilization composition containing amino-oligosaccharin and validamycin and application of pesticide composition | |
| CN107231982A (en) | Plant protection method and application | |
| CN102318638A (en) | Corn seed coating agent | |
| CN102633570A (en) | Special active biological medicine fertilizer for hot pepper | |
| Sathiyabama et al. | Suppression of dry root rot disease caused by Rhizoctonia bataticola (Taub.) Butler in chickpea plants by application of thiamine loaded chitosan nanoparticles | |
| CN102907458B (en) | Biological mosquitocide and preparation method thereof | |
| CN102388885A (en) | Chitosan oligosaccharide composition for improving quality of crops, as well as application and method thereof | |
| RU2216173C2 (en) | Method for obtaining preparation for presowing treatment of seeds and vegetating plants of agricultural varieties, fruit trees and berry bushes | |
| CN103875712A (en) | Safe pleietrepie rice seed treating agent | |
| CN107372615A (en) | A kind of seed dressing and its preparation method and application | |
| CN106719846A (en) | A kind of complex ecological agricultural chemicals and preparation method thereof | |
| CN106490049A (en) | A kind of composite pesticide for growing vegetables and preparation method thereof | |
| CN101569310B (en) | Formulation and application of green fruit/vegetable plant regulator | |
| KR101639552B1 (en) | Forest Ginseng Cultivated From Wild Ginseng Berry And Cultivation Method Thereof | |
| CN110754362B (en) | Culture medium and culture method of selenium-rich anoectochilus roxburghii | |
| CN102219602B (en) | Novel biocontrol and plant vigor complexing agent and preparation method thereof | |
| CN106386827A (en) | Plant disease bactericide high in safety and efficiency, and preparation method thereof | |
| CN106489995A (en) | A kind of biopesticide synergist and preparation method thereof | |
| Biswas et al. | Use of Amritpani: An Excellent Bio-Enhancer for Sustainable Agriculture: An Overview | |
| RU2640286C1 (en) | Fibre flax growing method | |
| Taimooz | Behavior of some nanomaterials in improving the growth of onion plant, Allium cepa and its effect on Pythium aphanidermatum. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171010 |
|
| RJ01 | Rejection of invention patent application after publication |