CN107227321A - A kind of mould proof agent and process for producing same of Progresses of Propionic Acid Production by Microbial Fermentation salt and purposes - Google Patents
A kind of mould proof agent and process for producing same of Progresses of Propionic Acid Production by Microbial Fermentation salt and purposes Download PDFInfo
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- CN107227321A CN107227321A CN201710406760.6A CN201710406760A CN107227321A CN 107227321 A CN107227321 A CN 107227321A CN 201710406760 A CN201710406760 A CN 201710406760A CN 107227321 A CN107227321 A CN 107227321A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 77
- 230000004151 fermentation Effects 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 62
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 230000000813 microbial effect Effects 0.000 title claims abstract description 32
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 29
- 235000019260 propionic acid Nutrition 0.000 title claims abstract description 29
- 150000003839 salts Chemical class 0.000 title abstract description 5
- 239000003795 chemical substances by application Substances 0.000 title abstract 2
- 239000007788 liquid Substances 0.000 claims abstract description 75
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims abstract description 65
- 241000186429 Propionibacterium Species 0.000 claims abstract description 45
- 239000003513 alkali Substances 0.000 claims abstract description 14
- 239000000706 filtrate Substances 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 9
- 239000007921 spray Substances 0.000 claims abstract description 9
- 239000003429 antifungal agent Substances 0.000 claims description 30
- 229940121375 antifungal agent Drugs 0.000 claims description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 19
- 239000000243 solution Substances 0.000 claims description 19
- 238000013190 sterility testing Methods 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000001110 calcium chloride Substances 0.000 claims description 12
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 12
- 235000013305 food Nutrition 0.000 claims description 10
- 241000894007 species Species 0.000 claims description 10
- 241000186426 Acidipropionibacterium acidipropionici Species 0.000 claims description 9
- 239000012670 alkaline solution Substances 0.000 claims description 9
- 238000001694 spray drying Methods 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 235000013339 cereals Nutrition 0.000 claims description 7
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930003270 Vitamin B Natural products 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 6
- 239000000498 cooling water Substances 0.000 claims description 6
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 229940074404 sodium succinate Drugs 0.000 claims description 6
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 6
- JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 6
- 235000019156 vitamin B Nutrition 0.000 claims description 6
- 239000011720 vitamin B Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 4
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 230000036512 infertility Effects 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 230000000855 fungicidal effect Effects 0.000 claims 1
- 239000000417 fungicide Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- RYCLIXPGLDDLTM-UHFFFAOYSA-J tetrapotassium;phosphonato phosphate Chemical compound [K+].[K+].[K+].[K+].[O-]P([O-])(=O)OP([O-])([O-])=O RYCLIXPGLDDLTM-UHFFFAOYSA-J 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 230000003449 preventive effect Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 5
- 238000005265 energy consumption Methods 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 description 2
- NBBJYMSMWIIQGU-UHFFFAOYSA-N Propionic aldehyde Chemical compound CCC=O NBBJYMSMWIIQGU-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000010331 calcium propionate Nutrition 0.000 description 2
- 239000004330 calcium propionate Substances 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- NFIYTPYOYDDLGO-UHFFFAOYSA-N phosphoric acid;sodium Chemical compound [Na].OP(O)(O)=O NFIYTPYOYDDLGO-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- XJMWHXZUIGHOBA-UHFFFAOYSA-N azane;propanoic acid Chemical compound N.CCC(O)=O XJMWHXZUIGHOBA-UHFFFAOYSA-N 0.000 description 1
- ZTQSAGDEMFDKMZ-UHFFFAOYSA-N butyric aldehyde Natural products CCCC=O ZTQSAGDEMFDKMZ-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- BWILYWWHXDGKQA-UHFFFAOYSA-M potassium propanoate Chemical compound [K+].CCC([O-])=O BWILYWWHXDGKQA-UHFFFAOYSA-M 0.000 description 1
- 235000010332 potassium propionate Nutrition 0.000 description 1
- 239000004331 potassium propionate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- XDWXRAYGALQIFG-UHFFFAOYSA-L zinc;propanoate Chemical compound [Zn+2].CCC([O-])=O.CCC([O-])=O XDWXRAYGALQIFG-UHFFFAOYSA-L 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B2/00—Preservation of foods or foodstuffs, in general
- A23B2/70—Preservation of foods or foodstuffs, in general by treatment with chemicals
- A23B2/725—Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
- A23B2/729—Organic compounds; Microorganisms; Enzymes
- A23B2/742—Organic compounds containing oxygen
- A23B2/754—Organic compounds containing oxygen containing carboxyl groups
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Abstract
Description
技术领域technical field
本发明属于饲料防霉剂技术领域，具体地，涉及一种微生物发酵法生产丙酸盐防霉剂生产方法及用途。The invention belongs to the technical field of antifungal agents for feed, and in particular relates to a production method and application of a propionate antifungal agent produced by microbial fermentation.
背景技术Background technique
丙酸及其盐如丙酸钙、丙酸锌、丙酸钾能有效地抑制霉菌、嗜氧芽孢杆菌，而且对人体基本无害，广泛应用在谷物、饲料和食品中。作为一种食品添加剂，是世界卫生组织（WHO）和联合国粮农组织（FAO）批准使用的安全可靠的食品与饲料用防霉剂，在食品工业和畜牧养殖业中使用极为普遍，其对霉菌、酵母菌及细菌等具有广泛的抗菌作用。Propionic acid and its salts such as calcium propionate, zinc propionate, and potassium propionate can effectively inhibit mold and aerobic bacillus, and are basically harmless to humans. They are widely used in grains, feed and food. As a food additive, it is a safe and reliable antifungal agent for food and feed approved by the World Health Organization (WHO) and the Food and Agriculture Organization of the United Nations (FAO). It is widely used in the food industry and animal husbandry. Yeast and bacteria have a wide range of antibacterial effects.
目前丙酸钙和丙酸铵的生产方法多为多用化学方法，但该方法有明显的缺点，如工艺流程复杂、设备繁多，对设备和管道材质的要求较高；反应条件苛刻，能耗高，对环境冲击大。At present, the production methods of calcium propionate and ammonium propionate are mostly multi-purpose chemical methods, but this method has obvious disadvantages, such as complex process flow, various equipment, high requirements for equipment and pipeline materials; harsh reaction conditions, high energy consumption , which has a large impact on the environment.
乳品源丙酸杆菌可以产生丙酸。通过微生物发酵可以生产很多的有机酸，如乳酸、柠檬酸等，且已工业化大生产。此类方法具有生产条件温和，能耗低、环境冲击小的特点。而能生产丙酸及其盐的微生物主要包括乳品源的丙酸杆菌，它们能够利用糖类物质在缺氧的条件下生产大量的丙酸或丙酸盐。另外，利用廉价的糖类物质生产丙酸或其盐，其成本将大大低于由丙醛为原料的化学合成法。Propionibacterium derived from dairy can produce propionic acid. Many organic acids can be produced through microbial fermentation, such as lactic acid, citric acid, etc., and have been industrially produced. This type of method has the characteristics of mild production conditions, low energy consumption, and small environmental impact. The microorganisms capable of producing propionic acid and its salts mainly include the dairy-derived Propionibacterium, which can use carbohydrates to produce a large amount of propionic acid or propionate under anoxic conditions. In addition, the cost of producing propionic acid or its salts by using cheap carbohydrates will be much lower than the chemical synthesis method using propionaldehyde as raw material.
发明内容Contents of the invention
针对现有技术中的缺陷，本发明的目的提供一种微生物发酵法生产丙酸盐防霉剂生产方法，所述方法需要的设备少，工艺流程简单，以替代化学合成法。In view of the defects in the prior art, the object of the present invention provides a method for the production of propionate antifungal agent by microbial fermentation. The method requires less equipment and simple process flow to replace the chemical synthesis method.
根据本发明一方面提供的一种微生物发酵法生产丙酸盐防霉剂生产方法，利用丙酸杆菌通过液态发酵生产丙酸盐，所述方法包括如下步骤：According to a method for producing propionate antifungal agent by microbial fermentation method provided in one aspect of the present invention, propionate is produced by liquid state fermentation using propionibacterium, and the method comprises the following steps:
（1）对丙酸杆菌进行种子扩大培养；(1) Carry out seed expansion cultivation of propionibacterium;
（2）制备丙酸杆菌的液体种；(2) Preparation of liquid species of Propionibacterium;
（3）接液体种于液体培养基进行发酵，生产丙酸；(3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;
（4）发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；(4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;
（5）当丙酸盐含量达到18-25克每升或每千克时，发酵结束；(5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;
（6）将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。(6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.
优选地，所述丙酸杆菌为产丙酸丙酸杆菌（Propionibacterium acidipropionici）DSM 25845。Preferably, the propionibacterium is Propionibacterium acidipropionici (Propionibacterium acidipropionici) DSM 25845.
优选地，所述步骤（1）中对丙酸杆菌进行种子扩大培养方法：Preferably, in the step (1), the method for seed expansion cultivation of propionibacterium:
（1）将菌种室制备的丙酸杆菌摇瓶种子接入到种子罐中培养，得到种子液，取样做无菌检测，其结果不含杂菌时，用冷却水使其降温，备用；(1) Connect the shake flask seeds of Propionibacterium prepared in the strain room to the seed tank for cultivation, obtain the seed liquid, take a sample for sterility testing, and if the result does not contain any bacteria, cool it with cooling water and set aside;
（2）将步骤（1）中制备的种子液分别移入到二级种子罐中，接种量为30-38L，经培养11h，得到OD=0.4-0.6的二级种子液，取样做无菌检测；(2) Transfer the seed liquid prepared in step (1) into the secondary seed tanks, the inoculum size is 30-38L, after culturing for 11 hours, obtain the secondary seed liquid with OD=0.4-0.6, and take samples for sterility test ;
（3）各个不含杂菌的二级种子液接入到其相对应的50m3三级种子罐中培养12-14h，得到OD=0.4-0.6的三级种子液，取样做无菌检测；(3) Put each second-level seed liquid without miscellaneous bacteria into its corresponding 50m 3 third-level seed tank and cultivate for 12-14 hours to obtain a third-level seed liquid with OD=0.4-0.6, and take samples for sterility testing;
（4）步骤（3）中得到不含杂菌的三级种子液分别接入相对应的500m3发酵罐中进行发酵生产，即可。(4) The tertiary seed liquid without miscellaneous bacteria obtained in step (3) is respectively put into corresponding 500m 3 fermenters for fermentation production.
优选地，所述步骤（1）扩大培养每L培养基含有：氯化钙0.5-0.7g、磷酸二氢钾0.5-1.0g、氯化铵0.5-1.0g、氯化钠1.5-2.0g、丁二酸钠0.5-1.0g、酵母粉0.1-0.4g、蛋白胨0.1-0.2g、维生素B10.003g、维生素B30.006g、硫酸锰1.0-2.0mg、硝酸铜0.02-0.04mg、硫酸锌0.1-0.3mg。Preferably, each L medium of step (1) expanded culture contains: calcium chloride 0.5-0.7g, potassium dihydrogen phosphate 0.5-1.0g, ammonium chloride 0.5-1.0g, sodium chloride 1.5-2.0g, Sodium succinate 0.5-1.0g, yeast powder 0.1-0.4g, peptone 0.1-0.2g, vitamin B 1 0.003g, vitamin B 3 0.006g, manganese sulfate 1.0-2.0mg, copper nitrate 0.02-0.04mg, zinc sulfate 0.1-0.3 mg.
优选地，所述步骤（2）液体培养基配方按重量百分比包括：5-15%白糖、5-8%甘油、1-3%玉米粉、2-5%氯化钙、0.1-0.3%磷酸二氢钠，其他为水，初始pH 6.1-6.4。Preferably, the formula of the liquid medium in step (2) includes by weight percentage: 5-15% white sugar, 5-8% glycerin, 1-3% corn flour, 2-5% calcium chloride, 0.1-0.3% phosphoric acid Sodium dihydrogen, others are water, initial pH 6.1-6.4.
优选地，所述步骤（3）碱溶液为氢氧化钠溶液或氢氧化钾溶液或氨水中的一种或多种。Preferably, the alkali solution in step (3) is one or more of sodium hydroxide solution, potassium hydroxide solution or ammonia water.
优选地，所述步骤（3）流加的速度为12-18ml/min。Preferably, the feeding speed in the step (3) is 12-18ml/min.
一方面提供的一种微生物发酵法生产丙酸盐防霉剂用途，所述微生物发酵法生产丙酸盐防霉剂用于谷物、饲料和食品的防霉。On the one hand, it provides a use of a microbial fermentation method to produce a propionate antifungal agent, and the microbial fermentation method produces a propionate antifungal agent for the antimold of grain, feed and food.
与现有技术相比，本发明具有如下的有益效果：Compared with the prior art, the present invention has the following beneficial effects:
（1）本发明提供一种微生物发酵法生产丙酸盐防霉剂生产方法，通过对丙酸杆菌进行种子扩大培养；制备丙酸杆菌的液体种；接液体种于液体培养基进行发酵，生产丙酸；发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；当丙酸盐含量达到18-25克每升或每千克时，发酵结束；将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。本方法只需要很少设备，工艺流程简单，方便易行。同时能耗低、原料来源广、价格低廉，因而大大降低生产成本；(1) The present invention provides a method for the production of propionate antifungal agent by microbial fermentation, by expanding the seed cultivation of Propionibacterium; preparing the liquid species of Propionibacterium; fermenting the liquid species in the liquid medium to produce Propionic acid; alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate; when the content of propionate reaches 18-25 grams per liter or per kilogram, the fermentation ends; the fermentation liquid is filtered through a filter press and taken The filtrate is sent to the spray tower for spray drying, and the dried powder is the propionate product. The method requires only a small amount of equipment, the process flow is simple, and it is convenient and easy to implement. At the same time, low energy consumption, wide sources of raw materials, and low prices greatly reduce production costs;
（2）本发明在发酵丙酸的过程中直接添加碱液，生成丙酸盐，不需要以化学合成的丙酸进行二次加工，脱离化学合成方法所产生的负面影响，节省了工艺流程，节省了成本。(2) In the process of fermenting propionic acid, the present invention directly adds lye to generate propionate, without the need for secondary processing of chemically synthesized propionic acid, which avoids the negative effects of chemical synthesis methods and saves the process flow. Cost savings.
具体实施方式detailed description
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.
本发明的目的提供一种微生物发酵法生产丙酸盐防霉剂生产方法，所述方法需要的设备少，工艺流程简单，以替代化学合成法。The object of the present invention provides a kind of microbial fermentation method to produce propionate antifungal agent production method, the equipment that described method needs is few, and technological process is simple, to replace chemical synthesis method.
根据本发明一方面提供的一种微生物发酵法生产丙酸盐防霉剂生产方法，利用丙酸杆菌通过液态发酵生产丙酸盐，所述方法包括如下步骤：According to a method for producing propionate antifungal agent by microbial fermentation method provided in one aspect of the present invention, propionate is produced by liquid state fermentation using propionibacterium, and the method comprises the following steps:
（1）对丙酸杆菌进行种子扩大培养；(1) Carry out seed expansion cultivation of propionibacterium;
（2）制备丙酸杆菌的液体种；(2) Preparation of liquid species of Propionibacterium;
（3）接液体种于液体培养基进行发酵，生产丙酸；(3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;
（4）发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；(4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;
（5）当丙酸盐含量达到18-25克每升或每千克时，发酵结束；(5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;
（6）将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。(6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.
优选地，所述丙酸杆菌为产丙酸丙酸杆菌（Propionibacteriumacidipropionici）DSM 25845。Preferably, the propionibacterium is Propionibacterium acidipropionici (Propionibacterium acidipropionici) DSM 25845.
优选地，所述步骤（1）中对丙酸杆菌进行种子扩大培养方法：Preferably, in the step (1), the method for seed expansion cultivation of propionibacterium:
（1）将菌种室制备的丙酸杆菌摇瓶种子接入到种子罐中培养，得到种子液，取样做无菌检测，其结果不含杂菌时，用冷却水使其降温，备用；(1) Connect the shake flask seeds of Propionibacterium prepared in the strain room to the seed tank for cultivation, obtain the seed liquid, take a sample for sterility testing, and if the result does not contain any bacteria, cool it with cooling water and set aside;
（2）将步骤（1）中制备的种子液分别移入到二级种子罐中，接种量为30-38L，经培养11h，得到OD=0.4-0.6的二级种子液，取样做无菌检测；(2) Transfer the seed liquid prepared in step (1) into the secondary seed tanks, the inoculum size is 30-38L, after culturing for 11 hours, obtain the secondary seed liquid with OD=0.4-0.6, and take samples for sterility test ;
（3）各个不含杂菌的二级种子液接入到其相对应的50m3三级种子罐中培养12-14h，得到OD=0.4-0.6的三级种子液，取样做无菌检测；(3) Put each secondary seed solution free of bacteria into its corresponding 50m 3 tertiary seed tank and cultivate for 12-14h to obtain a tertiary seed solution with OD=0.4-0.6, and take samples for sterility testing;
（4）步骤（3）中得到不含杂菌的三级种子液分别接入相对应的500m3发酵罐中进行发酵生产，即可。(4) The tertiary seed liquid without miscellaneous bacteria obtained in step (3) is respectively put into corresponding 500m 3 fermenters for fermentation production.
优选地，所述步骤（1）扩大培养每L培养基含有：氯化钙0.5-0.7g、磷酸二氢钾0.5-1.0g、氯化铵0.5-1.0g、氯化钠1.5-2.0g、丁二酸钠0.5-1.0g、酵母粉0.1-0.4g、蛋白胨0.1-0.2g、维生素B10.003g、维生素B30.006g、硫酸锰1.0-2.0mg、硝酸铜0.02-0.04mg、硫酸锌0.1-0.3mg。Preferably, each L medium of step (1) expanded culture contains: calcium chloride 0.5-0.7g, potassium dihydrogen phosphate 0.5-1.0g, ammonium chloride 0.5-1.0g, sodium chloride 1.5-2.0g, Sodium succinate 0.5-1.0g, yeast powder 0.1-0.4g, peptone 0.1-0.2g, vitamin B 1 0.003g, vitamin B 3 0.006g, manganese sulfate 1.0-2.0mg, copper nitrate 0.02-0.04mg, zinc sulfate 0.1-0.3 mg.
优选地，所述步骤（2）液体培养基配方按重量百分比包括：5-15%白糖、5-8%甘油、1-3%玉米粉、2-5%氯化钙、0.1-0.3%磷酸二氢钠，其他为水，初始pH 6.1-6.4。Preferably, the formula of the liquid medium in step (2) includes by weight percentage: 5-15% white sugar, 5-8% glycerin, 1-3% corn flour, 2-5% calcium chloride, 0.1-0.3% phosphoric acid Sodium dihydrogen, others are water, initial pH 6.1-6.4.
优选地，所述步骤（3）碱溶液为氢氧化钠溶液或氢氧化钾溶液或氨水中的一种或多种。Preferably, the alkali solution in step (3) is one or more of sodium hydroxide solution, potassium hydroxide solution or ammonia water.
优选地，所述步骤（3）流加的速度为12-18ml/min。Preferably, the feeding speed in the step (3) is 12-18ml/min.
一方面提供的一种微生物发酵法生产丙酸盐防霉剂用途，所述微生物发酵法生产丙酸盐防霉剂用于谷物、饲料和食品的防霉。On the one hand, it provides a use of a microbial fermentation method to produce a propionate antifungal agent, and the microbial fermentation method produces a propionate antifungal agent for the antimold of grain, feed and food.
与现有技术相比，本发明具有如下的有益效果：Compared with the prior art, the present invention has the following beneficial effects:
（1）本发明提供一种微生物发酵法生产丙酸盐防霉剂生产方法，通过对丙酸杆菌进行种子扩大培养；制备丙酸杆菌的液体种；接液体种于液体培养基进行发酵，生产丙酸；发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；当丙酸盐含量达到18-25克每升或每千克时，发酵结束；将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。本方法只需要很少设备，工艺流程简单，方便易行。同时能耗低、原料来源广、价格低廉，因而大大降低生产成本；(1) The present invention provides a method for the production of propionate antifungal agent by microbial fermentation, by expanding the seed cultivation of Propionibacterium; preparing the liquid species of Propionibacterium; fermenting the liquid species in the liquid medium to produce Propionic acid; alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate; when the content of propionate reaches 18-25 grams per liter or per kilogram, the fermentation ends; the fermentation liquid is filtered through a filter press and taken The filtrate is sent to the spray tower for spray drying, and the dried powder is the propionate product. The method requires only a small amount of equipment, the process flow is simple, and it is convenient and easy to implement. At the same time, low energy consumption, wide sources of raw materials, and low prices greatly reduce production costs;
（2）本发明在发酵丙酸的过程中直接添加碱液，生成丙酸盐，不需要以化学合成的丙酸进行二次加工，脱离化学合成方法所产生的负面影响，节省了工艺流程，节省了成本。(2) In the process of fermenting propionic acid, the present invention directly adds lye to generate propionate, without the need for secondary processing of chemically synthesized propionic acid, which avoids the negative effects of chemical synthesis methods and saves the process flow. Cost savings.
实施例1Example 1
本实施例一方面提供的一种微生物发酵法生产丙酸盐防霉剂生产方法，利用丙酸杆菌通过液态发酵生产丙酸盐，所述方法包括如下步骤：One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:
（1）对丙酸杆菌进行种子扩大培养；(1) Carry out seed expansion cultivation of propionibacterium;
（2）制备丙酸杆菌的液体种；(2) Preparation of liquid species of Propionibacterium;
（3）接液体种于液体培养基进行发酵，生产丙酸；(3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;
（4）发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；(4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;
（5）当丙酸盐含量达到18-25克每升或每千克时，发酵结束；(5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;
（6）将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。(6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.
所述丙酸杆菌为产丙酸丙酸杆菌（Propionibacterium acidipropionici）DSM25845。The propionibacterium is Propionibacterium acidipropionici DSM25845.
所述步骤（1）中对丙酸杆菌进行种子扩大培养方法：In the step (1), the method for expanding the cultivation of propionibacterium seeds:
（1）将菌种室制备的丙酸杆菌摇瓶种子接入到种子罐中培养，得到种子液，取样做无菌检测，其结果不含杂菌时，用冷却水使其降温，备用；(1) Connect the shake flask seeds of Propionibacterium prepared in the strain room to the seed tank for cultivation, obtain the seed liquid, take a sample for sterility testing, and if the result does not contain any bacteria, cool it with cooling water and set aside;
（2）将步骤（1）中制备的种子液分别移入到二级种子罐中，接种量为38L，经培养11h，得到OD=0.4的二级种子液，取样做无菌检测；(2) Transfer the seed liquid prepared in step (1) into secondary seed tanks with an inoculum size of 38 L. After culturing for 11 hours, obtain a secondary seed liquid with OD=0.4, and take samples for sterility testing;
（3）各个不含杂菌的二级种子液接入到其相对应的50m3三级种子罐中培养14h，得到OD=0.4的三级种子液，取样做无菌检测；(3) Put each second-grade seed liquid without miscellaneous bacteria into its corresponding 50m 3 third-grade seed tank and cultivate for 14 hours to obtain a third-grade seed liquid with OD=0.4, and take samples for sterility testing;
（4）步骤（3）中得到不含杂菌的三级种子液分别接入相对应的500m3发酵罐中进行发酵生产，即可。(4) The tertiary seed liquid without miscellaneous bacteria obtained in step (3) is respectively put into corresponding 500m 3 fermenters for fermentation production.
所述步骤（1）扩大培养每L培养基含有：氯化钙0.7g、磷酸二氢钾0.5g、氯化铵1.0g、氯化钠1.5g、丁二酸钠1.0g、酵母粉0.1g、蛋白胨0.2g、维生素B10.003g、维生素B30.006g、硫酸锰1.0-2.0mg、硝酸铜0.02-0.04mg、硫酸锌0.1-0.3mg。In the step (1), each L culture medium contains: calcium chloride 0.7g, potassium dihydrogen phosphate 0.5g, ammonium chloride 1.0g, sodium chloride 1.5g, sodium succinate 1.0g, yeast powder 0.1g , peptone 0.2g, vitamin B 1 0.003g, vitamin B 3 0.006g, manganese sulfate 1.0-2.0mg, copper nitrate 0.02-0.04mg, zinc sulfate 0.1-0.3mg.
所述步骤（2）液体培养基配方按重量百分比包括：15%白糖、5%甘油、3%玉米粉、2%氯化钙、0.3%磷酸二氢钠，其他为水，初始pH 6.1-6.4。The formula of the liquid medium in the step (2) includes by weight percentage: 15% white sugar, 5% glycerin, 3% corn flour, 2% calcium chloride, 0.3% sodium dihydrogen phosphate, the others are water, and the initial pH is 6.1-6.4 .
所述步骤（3）碱溶液为氢氧化钠溶液或氢氧化钾溶液或氨水中的一种或多种。The alkali solution in the step (3) is one or more of sodium hydroxide solution, potassium hydroxide solution or ammonia water.
所述步骤（3）流加的速度为18ml/min。The feeding speed in the step (3) is 18ml/min.
一方面提供的一种微生物发酵法生产丙酸盐防霉剂用途，所述微生物发酵法生产丙酸盐防霉剂用于谷物、饲料和食品的防霉。On the one hand, it provides a use of a microbial fermentation method to produce a propionate antifungal agent, and the microbial fermentation method produces a propionate antifungal agent for the antimold of grain, feed and food.
实施例2Example 2
本实施例一方面提供的一种微生物发酵法生产丙酸盐防霉剂生产方法，利用丙酸杆菌通过液态发酵生产丙酸盐，所述方法包括如下步骤：One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:
（1）对丙酸杆菌进行种子扩大培养；(1) Carry out seed expansion cultivation of propionibacterium;
（2）制备丙酸杆菌的液体种；(2) Preparation of liquid species of Propionibacterium;
（3）接液体种于液体培养基进行发酵，生产丙酸；(3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;
（4）发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；(4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;
（5）当丙酸盐含量达到18-25克每升或每千克时，发酵结束；(5) When the propionate content reaches 18-25 grams per liter or kilogram, the fermentation ends;
（6）将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。(6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.
所述丙酸杆菌为产丙酸丙酸杆菌（Propionibacterium acidipropionici）DSM25845。The propionibacterium is Propionibacterium acidipropionici DSM25845.
所述步骤（1）中对丙酸杆菌进行种子扩大培养方法：In the step (1), the method for expanding the cultivation of propionibacterium seeds:
（1）将菌种室制备的丙酸杆菌摇瓶种子接入到种子罐中培养，得到种子液，取样做无菌检测，其结果不含杂菌时，用冷却水使其降温，备用；(1) Connect the shake flask seeds of Propionibacterium prepared in the strain room to the seed tank for cultivation, obtain the seed liquid, take a sample for sterility testing, and if the result does not contain any bacteria, cool it with cooling water and set aside;
（2）将步骤（1）中制备的种子液分别移入到二级种子罐中，接种量为30L，经培养11h，得到OD=0.6的二级种子液，取样做无菌检测；(2) Transfer the seed solution prepared in step (1) into secondary seed tanks with an inoculum volume of 30 L. After culturing for 11 hours, obtain a secondary seed solution with OD=0.6, and take samples for sterility testing;
（3）各个不含杂菌的二级种子液接入到其相对应的50m3三级种子罐中培养12h，得到OD=0.6的三级种子液，取样做无菌检测；(3) Put each second-level seed liquid without miscellaneous bacteria into its corresponding 50m 3 third-level seed tank and cultivate for 12 hours to obtain a third-level seed liquid with OD=0.6, and take samples for sterility testing;
（4）步骤（3）中得到不含杂菌的三级种子液分别接入相对应的500m3发酵罐中进行发酵生产，即可。(4) The tertiary seed liquid without miscellaneous bacteria obtained in step (3) is respectively put into corresponding 500m 3 fermenters for fermentation production.
所述步骤（1）扩大培养每L培养基含有：氯化钙0.5g、磷酸二氢钾1.0g、氯化铵0.5g、氯化钠2.0g、丁二酸钠0.5g、酵母粉0.4g、蛋白胨0.1g、维生素B10.003g、维生素B30.006g、硫酸锰1.0-2.0mg、硝酸铜0.02-0.04mg、硫酸锌0.1-0.3mg。In the step (1), each L culture medium contains: calcium chloride 0.5g, potassium dihydrogen phosphate 1.0g, ammonium chloride 0.5g, sodium chloride 2.0g, sodium succinate 0.5g, yeast powder 0.4g , peptone 0.1g, vitamin B 1 0.003g, vitamin B 3 0.006g, manganese sulfate 1.0-2.0mg, copper nitrate 0.02-0.04mg, zinc sulfate 0.1-0.3mg.
所述步骤（2）液体培养基配方按重量百分比包括：5%白糖、8%甘油、1%玉米粉、5%氯化钙、0.1%磷酸二氢钠，其他为水，初始pH 6.1-6.4。The formula of the liquid medium in the step (2) includes by weight percentage: 5% white sugar, 8% glycerin, 1% corn flour, 5% calcium chloride, 0.1% sodium dihydrogen phosphate, the others are water, and the initial pH is 6.1-6.4 .
所述步骤（3）碱溶液为氢氧化钠溶液或氢氧化钾溶液或氨水中的一种或多种。The alkali solution in the step (3) is one or more of sodium hydroxide solution, potassium hydroxide solution or ammonia water.
所述步骤（3）流加的速度为12ml/min。The feeding speed in the step (3) is 12ml/min.
一方面提供的一种微生物发酵法生产丙酸盐防霉剂用途，所述微生物发酵法生产丙酸盐防霉剂用于谷物、饲料和食品的防霉。On the one hand, it provides a use of a microbial fermentation method to produce a propionate antifungal agent, and the microbial fermentation method produces a propionate antifungal agent for the antimold of grain, feed and food.
实施例3Example 3
本实施例一方面提供的一种微生物发酵法生产丙酸盐防霉剂生产方法，利用丙酸杆菌通过液态发酵生产丙酸盐，所述方法包括如下步骤：One aspect of this embodiment provides a method for producing propionate antifungal agent by microbial fermentation, using propionibacterium to produce propionate through liquid fermentation, and the method includes the following steps:
（1）对丙酸杆菌进行种子扩大培养；(1) Carry out seed expansion cultivation of propionibacterium;
（2）制备丙酸杆菌的液体种；(2) Preparation of liquid species of Propionibacterium;
（3）接液体种于液体培养基进行发酵，生产丙酸；(3) Connect the liquid seeds to the liquid medium for fermentation to produce propionic acid;
（4）发酵过程流加碱溶液，丙酸与碱反应得到丙酸盐；(4) Alkaline solution is added to the fermentation process, and propionic acid reacts with alkali to obtain propionate;
（5）当丙酸盐含量达到22克每升或每千克时，发酵结束；(5) Fermentation ends when the propionate content reaches 22 grams per liter or kilogram;
（6）将发酵液经过压滤机过滤、取滤液，送入喷雾塔进行喷雾干燥，干燥后的粉剂即为丙酸盐制品。(6) Filter the fermentation liquid through a filter press, take the filtrate, and send it to the spray tower for spray drying. The dried powder is the propionate product.
所述丙酸杆菌为产丙酸丙酸杆菌（Propionibacterium acidipropionici）DSM25845。The propionibacterium is Propionibacterium acidipropionici DSM25845.
所述步骤（1）中对丙酸杆菌进行种子扩大培养方法：In the step (1), the method for expanding the cultivation of propionibacterium seeds:
（1）将菌种室制备的丙酸杆菌摇瓶种子接入到种子罐中培养，得到种子液，取样做无菌检测，其结果不含杂菌时，用冷却水使其降温，备用；(1) Connect the shake flask seeds of Propionibacterium prepared in the strain room to the seed tank for cultivation, obtain the seed liquid, take a sample for sterility testing, and if the result does not contain any bacteria, cool it with cooling water and set aside;
（2）将步骤（1）中制备的种子液分别移入到二级种子罐中，接种量为35L，经培养11h，得到OD=0.5的二级种子液，取样做无菌检测；(2) Transfer the seed solution prepared in step (1) into secondary seed tanks with an inoculum size of 35 L. After culturing for 11 hours, obtain a secondary seed solution with OD=0.5, and take samples for sterility testing;
（3）各个不含杂菌的二级种子液接入到其相对应的50m3三级种子罐中培养13h，得到OD=0.5的三级种子液，取样做无菌检测；(3) Put each second-level seed liquid without miscellaneous bacteria into its corresponding 50m 3 third-level seed tank and cultivate for 13 hours to obtain a third-level seed liquid with OD=0.5, and take samples for sterility testing;
（4）步骤（3）中得到不含杂菌的三级种子液分别接入相对应的500m3发酵罐中进行发酵生产，即可。(4) The tertiary seed liquid without miscellaneous bacteria obtained in step (3) is respectively put into corresponding 500m 3 fermenters for fermentation production.
所述步骤（1）扩大培养每L培养基含有：氯化钙0.6g、磷酸二氢钾0.8g、氯化铵0.9g、氯化钠1.7g、丁二酸钠0.9g、酵母粉0.3g、蛋白胨0.1g、维生素B10.003g、维生素B30.006g、硫酸锰1.0-2.0mg、硝酸铜0.02-0.04mg、硫酸锌0.1-0.3mg。In the step (1), each L culture medium contains: calcium chloride 0.6g, potassium dihydrogen phosphate 0.8g, ammonium chloride 0.9g, sodium chloride 1.7g, sodium succinate 0.9g, yeast powder 0.3g , peptone 0.1g, vitamin B 1 0.003g, vitamin B 3 0.006g, manganese sulfate 1.0-2.0mg, copper nitrate 0.02-0.04mg, zinc sulfate 0.1-0.3mg.
所述步骤（2）液体培养基配方按重量百分比包括：12%白糖、6%甘油、2%玉米粉、3%氯化钙、0.2%磷酸二氢钠，其他为水，初始pH 6.1-6.4。The formula of the liquid medium in the step (2) includes by weight percentage: 12% white sugar, 6% glycerin, 2% corn flour, 3% calcium chloride, 0.2% sodium dihydrogen phosphate, the others are water, and the initial pH is 6.1-6.4 .
所述步骤（3）碱溶液为氢氧化钠溶液或氢氧化钾溶液或氨水中的一种或多种。The alkali solution in the step (3) is one or more of sodium hydroxide solution, potassium hydroxide solution or ammonia water.
所述步骤（3）流加的速度为17ml/min。The feeding speed in the step (3) is 17ml/min.
一方面提供的一种微生物发酵法生产丙酸盐防霉剂用途，所述微生物发酵法生产丙酸盐防霉剂用于谷物、饲料和食品的防霉。On the one hand, it provides a use of a microbial fermentation method to produce a propionate antifungal agent, and the microbial fermentation method produces a propionate antifungal agent for the antimold of grain, feed and food.
以上对本发明的具体实施例进行了描述。需要理解的是，本发明并不局限于上述特定实施方式，本领域技术人员可以在权利要求的范围内做出各种变形或修改，这并不影响本发明的实质内容。Specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art may make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902649A (en) * | 2018-07-27 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of preparation method of microbial food mould inhibitor |
| CN111549080A (en) * | 2020-06-12 | 2020-08-18 | 陕西科技大学 | Fermentation production process of zinc propionate |
| CN112359068A (en) * | 2020-10-19 | 2021-02-12 | 安徽绿微康生物科技有限公司 | Fermentation preservative and preparation method and application thereof |
| CN112359069A (en) * | 2020-10-20 | 2021-02-12 | 安徽绿微康生物科技有限公司 | Microbial leavening agent and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101748163A (en) * | 2009-12-25 | 2010-06-23 | 朝阳华星生物工程有限公司 | Method of producing propionic acid and propionate by microorganism fermentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101748163A (en) * | 2009-12-25 | 2010-06-23 | 朝阳华星生物工程有限公司 | Method of producing propionic acid and propionate by microorganism fermentation |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902649A (en) * | 2018-07-27 | 2018-11-30 | 苏州汉德瑞生物工程有限公司 | A kind of preparation method of microbial food mould inhibitor |
| CN111549080A (en) * | 2020-06-12 | 2020-08-18 | 陕西科技大学 | Fermentation production process of zinc propionate |
| CN112359068A (en) * | 2020-10-19 | 2021-02-12 | 安徽绿微康生物科技有限公司 | Fermentation preservative and preparation method and application thereof |
| CN112359069A (en) * | 2020-10-20 | 2021-02-12 | 安徽绿微康生物科技有限公司 | Microbial leavening agent and preparation method and application thereof |
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