CN107213806B - A kind of filter membrane for filtrating leukocytes and preparation method thereof - Google Patents
A kind of filter membrane for filtrating leukocytes and preparation method thereof Download PDFInfo
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- CN107213806B CN107213806B CN201710571716.0A CN201710571716A CN107213806B CN 107213806 B CN107213806 B CN 107213806B CN 201710571716 A CN201710571716 A CN 201710571716A CN 107213806 B CN107213806 B CN 107213806B
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- 239000012528 membrane Substances 0.000 title claims abstract description 82
- 210000000265 leukocyte Anatomy 0.000 title claims abstract description 72
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- -1 nano zine oxide Chemical compound 0.000 claims abstract description 29
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000000661 sodium alginate Substances 0.000 claims abstract description 23
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 23
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 23
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000003756 stirring Methods 0.000 claims description 20
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 19
- 229910052708 sodium Inorganic materials 0.000 claims description 19
- 239000011734 sodium Substances 0.000 claims description 19
- 125000003368 amide group Chemical group 0.000 claims description 13
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims description 12
- 238000000151 deposition Methods 0.000 claims description 12
- 230000008021 deposition Effects 0.000 claims description 12
- 229920005610 lignin Polymers 0.000 claims description 12
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 12
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 10
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000011701 zinc Substances 0.000 claims description 8
- 229910052725 zinc Inorganic materials 0.000 claims description 8
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- BHTJEPVNHUUIPV-UHFFFAOYSA-N pentanedial;hydrate Chemical compound O.O=CCCCC=O BHTJEPVNHUUIPV-UHFFFAOYSA-N 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- 229960001124 trientine Drugs 0.000 claims description 5
- OKKRPWIIYQTPQF-UHFFFAOYSA-N Trimethylolpropane trimethacrylate Chemical compound CC(=C)C(=O)OCC(CC)(COC(=O)C(C)=C)COC(=O)C(C)=C OKKRPWIIYQTPQF-UHFFFAOYSA-N 0.000 claims description 4
- 150000001412 amines Chemical class 0.000 claims description 3
- 230000001186 cumulative effect Effects 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 3
- 230000006855 networking Effects 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 2
- 238000009987 spinning Methods 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 235000010443 alginic acid Nutrition 0.000 claims 1
- 239000000783 alginic acid Substances 0.000 claims 1
- 229960001126 alginic acid Drugs 0.000 claims 1
- 229920000615 alginic acid Polymers 0.000 claims 1
- 150000004781 alginic acids Chemical class 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 239000012259 ether extract Substances 0.000 claims 1
- 239000003208 petroleum Substances 0.000 claims 1
- 238000005303 weighing Methods 0.000 claims 1
- 210000004369 blood Anatomy 0.000 abstract description 52
- 239000008280 blood Substances 0.000 abstract description 49
- 238000001914 filtration Methods 0.000 abstract description 37
- 210000001772 blood platelet Anatomy 0.000 abstract description 17
- 239000000463 material Substances 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 11
- 230000001194 anti-hemostatic effect Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000004520 agglutination Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 21
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 10
- 239000011787 zinc oxide Substances 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 238000011109 contamination Methods 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229920002635 polyurethane Polymers 0.000 description 3
- 239000004814 polyurethane Substances 0.000 description 3
- 230000010148 water-pollination Effects 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000005576 amination reaction Methods 0.000 description 2
- 230000000702 anti-platelet effect Effects 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000024908 graft versus host disease Diseases 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000002615 hemofiltration Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- SYMAIKWDBCFRMP-UHFFFAOYSA-N C(C=C)(=O)O.C(C=C)(=O)OCCCO.C(C=C)(=O)O Chemical compound C(C=C)(=O)O.C(C=C)(=O)OCCCO.C(C=C)(=O)O SYMAIKWDBCFRMP-UHFFFAOYSA-N 0.000 description 1
- 108010022355 Fibroins Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 208000009329 Graft vs Host Disease Diseases 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical group COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 208000003441 Transfusion reaction Diseases 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000012675 alcoholic extract Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000003914 blood derivative Substances 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000003617 erythrocyte membrane Anatomy 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 239000012051 hydrophobic carrier Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000012633 leachable Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- GRKXKNLRBZVLSN-UHFFFAOYSA-N methylamino acetate Chemical compound CNOC(C)=O GRKXKNLRBZVLSN-UHFFFAOYSA-N 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000001792 thromboblast Anatomy 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
- B01D71/06—Organic material
- B01D71/74—Natural macromolecular material or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
- A61M1/3496—Plasmapheresis; Leucopheresis; Lymphopheresis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
- B01D67/0079—Manufacture of membranes comprising organic and inorganic components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
- C01G9/00—Compounds of zinc
- C01G9/02—Oxides; Hydroxides
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/60—Particles characterised by their size
- C01P2004/64—Nanometer sized, i.e. from 1-100 nanometer
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Manufacturing & Machinery (AREA)
- Life Sciences & Earth Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Physics & Mathematics (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Biochemistry (AREA)
- Vascular Medicine (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Hematology (AREA)
- Composite Materials (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention belongs to leukoreduction filter technical field, and in particular to a kind of filter membrane for filtrating leukocytes and preparation method thereof.Mainly it is made up of provided by the present invention for the filter membrane of filtrating leukocytes sodium alginate, nano zine oxide, chitosan oligosaccharide and amido sodium lignin sulfonate.The filter membrane for filtrating leukocytes that the present invention is prepared has adhesion by force and the advantages of stability is high, and material on filter membrane can be avoided to wash out or come off in use, effectively can be polluted anti-Hemostatic Oral Liquid；Simultaneously, have leucocyte and blood platelet filtration rate high provided by the present invention for the filter membrane of filtrating leukocytes, there is alleviation or anti-Hemostatic Oral Liquid cold agglutination phenomenon occurs during blood filtration, dredge the effect that filter sizes block, the filter quality of blood leucocyte can be ensured, the service life length of leukocyte filter membranes can also be extended, be a kind of ideal leukoreduction filter film.
Description
Technical field
The invention belongs to leukoreduction filter technical field, and in particular to a kind of filter membrane and its preparation for filtrating leukocytes
Method.
Background technology
In medical procedure of transfusing blood, a variety of transfusion adverse effects can usually occur, its root is whole blood of the input containing leucocyte
Or blood constituent can cause nonhemolytic febrific reaction, HLA isoimmunization, blood transfusion correlated virus infection and it is defeated
The Adverse transfusion reaction such as graft versus host disease(GVH disease) after blood.Therefore, filtered out before blood transfusion the leucocyte in Blood Preparations have it is important
Meaning.Leucocyte filter is the important method of filtrating leukocytes, and its principle is cut using the principle of machinery retardance and absorption
Cell is left white, and allows the red blood cell that surface is smooth, deformability is stronger to pass through.At present, the requirement of the 3rd generation leucocyte filter is
Rejection rate reaches 99.99%.
In addition, modern study shows that blood platelet can also induce body to produce antiplatelet antibody, antiplatelet antibody is one
Kind autoantibody, the histoorgan nonrecognition to itself, can attack the histoorgan of itself, so as to cause damage.Therefore, in order to
The side effect caused by platelet transfusion is reduced, people are directed to research one kind and also can expeditiously removed while except leucocyte-removing
Remove hematoblastic filter membrane.There is correlative study to attempt the fibre diameter by reducing filter medium or aperture or increase filtering Jie in the past
The packed density of matter removes the means of leucocyte-removing and blood platelet ability as filter medium is improved simultaneously.But apply the above method
There can be hemofiltration process time lengthening and may result in erythrocyte membrane rupture and haemolysis occur.
At present, leukocyte filter membranes are changed using surface grafting or coating hydrophilic polymer to filtering material surface more
Property, increase the wetability of filtering material, reduce the surface energy of filtering material surface and blood, increase filtering material contact blood
Effective surface area, so as to increase the chance for catching leucocyte.But the hydrophily on filtering material surface is higher, blood platelet is more difficult to
Activation, it can be easy to form water layer in material surface additionally by hydrogen bond of water and material etc., have and suppress plasma protein suction
It is attached, especially fibrinous absorption, so as to reduce hematoblastic adhesiveness, hematoblastic retention is reduced.In addition, hydrophily
The water solubility of polymer is stronger, dissolution in blood can be present.
Patent document CN103415309B discloses a kind of for removing material from blood or from blood derivatives
Improved filter and method for obtaining the filter.Described filter includes the filter elements for accommodating stratiform
The housing of part, layered filter cell include multiple layers, and at least one layer of layered filter cell is coated with
Polyurethane, the molecular weight of the polyurethane are 12,000 dalton to 18,000 dalton, and are coated with the non-of polyurethane and knit
The divine force that created the universe is mixed in the stratiform filter element formed in the less weaven goods of hydrophily, described stratiform filter element to leucocyte and
Blood platelet, which is respectively provided with, preferably filters out effect.
Patent document CN106117580A discloses a kind of filter membrane modifying agent and its method of modifying for filtrating leukocytes,
The filter membrane modifying agent modifies fibroin albumen, polyvinylpyrrolidone and acrylate copolymer with (0.052) by amidatioon:
(0.2~1):1 mass ratio composition.Wherein, acrylate copolymer is by methyl methacrylate monomer and methacrylic acid two
Methylamino acetate monomer is formed.The filter membrane modifying agent being prepared, which is dissolved in polar solvent such as water, is configured to solution, with infiltration
Modified mode is coated processing to filter membrane, and the filter membrane after filter membrane modifier treatment has preferably wetting
Property, stability and compatibility, higher rejection rate is respectively provided with to leucocyte and blood platelet, and wash out with relatively low modifying agent
Rate, leachable is avoided to pollute.
However, current leukocyte filter membranes only focus on the design of cell rate filtering, tended to out during use
Existing filter membrane is blocked the defects of causing service life relatively low or influence cell filtration quality, meanwhile, during filtering easily
There is the pollution of blood, cause to bring bacterium inside receptor into, larger threat be present to the health of receptor.
The content of the invention
In order to overcome the defects of leukocyte filter membranes are present in the prior art.It is used to filter it is an object of the invention to provide one kind
Filter membrane except leucocyte and preparation method thereof, to solve drawbacks described above.
The invention provides a kind of filter membrane for filtrating leukocytes, comprise the following steps：
100~200 parts of sodium alginate, 35~45 parts of nano zine oxide, 15~25 parts of chitosan oligosaccharide and amido lignin sulfonic acid
10~20 parts of sodium.
Further, the filter membrane for filtrating leukocytes includes following preparing raw material and its parts by weight：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and amido sodium lignin sulfonate.
Further, the preparation method of the nano zine oxide is：
A. weigh zinc nitrate to add in deionized water, stir to being completely dissolved, be subsequently added into sodium carbonate, stirring is to having reacted
Entirely, centrifuge, obtain white depositions；
B. lauryl glucosyl is added toward the obtained white depositions of step a, stirs 5~8min, be subsequently added into three
TRIM, 10~20min is stirred, obtains mixed liquor；
C. the obtained mixed liquors of step b being heated to 130~140 DEG C, insulation stands 30~40min, is then passed through nitrogen,
Continue to be heated to 250~300 DEG C, insulation stands 40~60min；
D. the obtained mixed liquors of step c are cooled to 35~45 DEG C, add absolute ethyl alcohol, centrifuge to obtain sediment, then with nothing
Water-ethanol washs, and centrifuges, and dries, and obtains the nano zine oxide that particle diameter is 8~10nm.
Further, the weight of zinc nitrate and sodium carbonate ratio is (1.2~1.6) in the step a:1.
Further, white depositions and the solid-to-liquid ratio of lauryl glucosyl are 1g in the step b：(10~12)
ml。
Further, white depositions and the solid-to-liquid ratio of trimethylol-propane trimethacrylate are in the step b
1g：(6~8) ml.
Further, the preparation method of the amido sodium lignin sulfonate is：
A. it is 65~75% sodium lignin sulfonate water by the sodium lignin sulfonate mass percent concentration soluble in water that is configured to
Solution, 60~80 DEG C are heated to, add triethylene tetramine, it is water-soluble that the glutaraldehyde that mass percent concentration is 50% is then added dropwise
Liquid, the drop rate of the glutaraldehyde water solution are 0.3~0.5mL/min, and time for adding control is in 20~30min, in temperature
For 80~90 DEG C of 2~3h of reaction, amido lignin sulfonic acid sodium solution is obtained；
B. the amido lignin sulfonic acid sodium solution that step A is obtained is stripped with absolute ethyl alcohol, obtains extract, use oil
Ether washs extract, is freeze-dried, produced after washing.
Further, the addition of the triethylene tetramine in the step A is lignin sulfonic acid sodium water solution cumulative volume
20~30%.
Further, the condition being freeze-dried in the step B is：- 8 DEG C of 2~4h of pre-freeze, it is then 0.04 in vacuum
~0.06mPa, temperature are -18 DEG C of 20~24h of freezing.
In addition, present invention also offers a kind of preparation method of the filter membrane for filtrating leukocytes, comprise the following steps：
Sodium alginate is added to the water by S1, stirs to being completely dissolved, obtains sodium alginate aqueous solution；
Chitosan oligosaccharide and amido sodium lignin sulfonate are added to the water by S2, stir to being completely dissolved, obtain mixed liquor；
Nano zine oxide is added to the water by S3, stirs to being completely dissolved, obtains nano oxidized zinc solution；
S4 mixes the mixed liquor that step S2 is obtained with the obtained sodium alginate aqueous solutions of step S1, stirs, must spin
Silk solution, the spinneret orifice for being then 0.08mm by aperture with measuring pump are squeezed into the nano oxidized zinc solution that step S3 is obtained,
Drawing-off, with 95% Ethanol Treatment fiber, centrifugation is sloughed drying fibrous after ethanol, is cut, is combed, networking, it is 4~3 μ that aperture, which is made,
M network structure, is produced.
Filter membrane provided by the present invention for filtrating leukocytes is the aqueous solution by chitosan oligosaccharide and amido sodium lignin sulfonate
Uniform spinning solution is formed after being blended with sodium alginate aqueous solution, is squeezed into burnett's solution and is prepared by spinneret orifice,
Solve in the prior art by the way that the effect that polymeric material is grafted or is bonded is coated on hydrophobic carrier, it is white thin in filtering
The polymer occurred during born of the same parents, which comes off, influences filter effect, or Leaching rule contaminated blood defect, and original provided by the invention
Material is nontoxic, free of contamination composition, is a kind of environmentally friendly, healthy, free of contamination leukocyte filter membranes.
Double filtration principle is used provided by the present invention for the filter membrane of filtrating leukocytes, can effectively improve leucocyte
With hematoblastic rejection rate.Aperture provided by the invention is the leukocyte filter membranes of 3~4 μm of network structure, can be prevented white thin
Born of the same parents by the way that while the positive charge amido of positively charged chitosan oligosaccharide and the amido sodium lignin sulfonate on filter membrane has to leucocyte
Very strong suction-operated, the two is acted on simultaneously, can effectively improve the rejection rate of leucocyte.In addition, embedded with self-control nano oxygen
The sodium alginate fiber for changing zinc can have suction-operated with activated blood platelet to blood platelet, also have, in amido sodium lignin sulfonate
The negatively charged groups such as the phenolic hydroxyl group that contains, alcoholic extract hydroxyl group, carbonyl, carboxyl can also activated blood platelet, also have to blood platelet and inhale
Attached effect, the two is acted on simultaneously, can effectively improve hematoblastic filter out.
Further, through experiment find, provided by the present invention for filtrating leukocytes filter membrane to the leucocyte in whole blood
Rejection rate be up to 99.999%, to hematoblastic rejection rate in whole blood up to more than 94%, it is white that its filtration result exceeded for the 3rd generation
The requirement of cell filter, it is a kind of efficient leucocyte and hematoblastic cell filtration film.
Provided by the present invention for the self-control nano zine oxide in the filter membrane of filtrating leukocytes, chitosan oligosaccharide and amination amido wood
The interaction of quality sodium sulfonate, which has, prevents filter membrane long-time from filtering generation filter opening and blocking, and alleviates and anti-Hemostatic Oral Liquid condenses the hair collected
Raw effect, solving the modern aperture that the leukocyte depletion filter that network structure forms is formed by synthetic microfibril can detention morphotropism
Poor cell, the red blood cell of modified difference and destroyed red blood cell are accumulated easily in leucocyte filter disc so that red in transfering bag
Cell can not be by filter disc, while due also to blood sample inventory time is longer, causes the deformation of the viscosity and red blood cell of blood
Property improve, the phenomenon of condensation collection occurs for blood, causes blood through occurring Severe blockage during leucocyte filter disc, causes filtering not smooth very
To not dripping, the defects of hemofiltration time lengthening or endless blood filtration, the filter quality of blood leucocyte can be effectively guaranteed.
Further, find through experiment, 400mL whole bloods were carried out provided by the present invention for the filter membrane of filtrating leukocytes
14~16min is only needed during filter, illustrating the leukocyte filter membranes of the offer of the present invention has preferable hydrophilic wettability, is advantageous to blood
The filtering of liquid.Meanwhile be 15~17min for the filtration time of the blood sample of exception, the time base with filtering normal blood
This holding is constant, illustrates self-control nano zine oxide, chitosan oligosaccharide and amine in the filter membrane provided by the present invention for filtrating leukocytes
Changing the interaction of amido sodium lignin sulfonate can prevent the aperture of filter membrane from blocking, and alleviate blood clotting phenomenon, can be effective
Expand the use range of leukocyte filter membranes and increase the service life.
In addition, the sodium alginate cellulose provided by the invention containing self-control nano zine oxide and chitosan oligosaccharide interaction tool
There is stronger antibacterial, sterilization, by germ contamination when can prevent blood filtration, while the fresh of blood can also be kept
Degree, the security of filtering blood can be improved.
Compared with prior art, had the advantage that provided by the present invention for the filter membrane of filtrating leukocytes：
(1) filter membrane provided by the present invention for filtrating leukocytes has the advantages of adhesion is strong and stability is high, makes
The material on filter membrane can be avoided to wash out or come off during, can effectively anti-Hemostatic Oral Liquid pollution；
(2) filter membrane provided by the present invention for filtrating leukocytes has leucocyte and blood platelet filtration rate high, in blood
There is alleviation or anti-Hemostatic Oral Liquid cold agglutination phenomenon, the effect that dredging filter sizes block occurs during filtering, it is ensured that
The filter quality of blood leucocyte, the service life length of leukocyte filter membranes can also be extended, be a kind of ideal leucocyte
Filter membrane.
Embodiment：
Below by way of the description of embodiment, the invention will be further described, but this is not the limit to the present invention
System, those skilled in the art according to the present invention basic thought, various modifications may be made or improve, but without departing from this
The basic thought of invention, within the scope of the present invention.
Embodiment 1, a kind of preparation of nano zine oxide
A. weigh zinc nitrate to add in deionized water, stir to being completely dissolved, be subsequently added into sodium carbonate, the zinc nitrate with
The weight ratio of sodium carbonate is 1.4:1, stirring centrifugation, obtains white depositions to reacting complete；
B. lauryl glucosyl is added toward the obtained white depositions of step a, stirs 6min, the white depositions
Solid-to-liquid ratio with lauryl glucosyl is 1g:12ml, trimethylol-propane trimethacrylate is subsequently added into, stirred
The solid-to-liquid ratio of 15min, the white depositions and trimethylol-propane trimethacrylate is 1g:8ml, obtain mixed liquor；
C. the obtained mixed liquors of step b are heated to 135 DEG C, insulation stands 35min, is then passed through nitrogen, continues to heat
To 280 DEG C, insulation stands 50min；
D. the obtained mixed liquors of step c are cooled to 40 DEG C, add absolute ethyl alcohol, centrifuge to obtain sediment, then with anhydrous second
Alcohol washs, and centrifuges, and dries, and obtains the nano zine oxide that particle diameter is 8~10nm.
Embodiment 2, a kind of preparation of amido sodium lignin sulfonate
A. it is 70% lignin sulfonic acid sodium water solution by the sodium lignin sulfonate mass percent concentration soluble in water that is configured to,
70 DEG C are heated to, adds triethylene tetramine, the addition of the triethylene tetramine is lignin sulfonic acid sodium water solution cumulative volume
25%, the glutaraldehyde water solution that mass percent concentration is 50% is then added dropwise, the drop rate of the glutaraldehyde water solution is
0.4mL/min, time for adding are controlled in 25min, are 85 DEG C of reaction 2h in temperature, are obtained amido lignin sulfonic acid sodium solution；
B. the amido lignin sulfonic acid sodium solution that step A is obtained is stripped with absolute ethyl alcohol, obtains extract, use oil
Ether washs extract, is then 0.05mPa in vacuum, temperature is -18 DEG C of freezing 22h, i.e., in -8 DEG C of pre-freeze 3h after washing
.
Embodiment 3, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
10 parts of 100 parts of sodium alginate, 35 parts of nano zine oxide, 15 parts of chitosan oligosaccharide and amido sodium lignin sulfonate；It is described to receive
Rice zinc oxide is nano zine oxide prepared by embodiment 1, and the amido sodium lignin sulfonate is that amido prepared by embodiment 2 is wooden
Plain sodium sulfonate.
Preparation method：
Sodium alginate is added to the water by S1, stirs to being completely dissolved, obtains sodium alginate aqueous solution；
Chitosan oligosaccharide and amido sodium lignin sulfonate are added to the water by S2, stir to being completely dissolved, obtain mixed liquor；
Nano zine oxide is added to the water by S3, stirs to being completely dissolved, obtains nano oxidized zinc solution；
S4 mixes the mixed liquor that step S2 is obtained with the obtained sodium alginate aqueous solutions of step S1, stirs, must spin
Silk solution, the spinneret orifice for being then 0.08mm by aperture with measuring pump are squeezed into the nano oxidized zinc solution that step S3 is obtained,
Drawing-off, with 95% Ethanol Treatment fiber, drying fibrous, cutting after ethanol is sloughed in centrifugation, and combing, networking, it is 3 μm that aperture, which is made,
Network structure, produce.
Embodiment 4, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and amido sodium lignin sulfonate；It is described to receive
Rice zinc oxide is nano zine oxide prepared by embodiment 1, and the amido sodium lignin sulfonate is that amido prepared by embodiment 2 is wooden
Plain sodium sulfonate.
Preparation method is similar to Example 3.
Embodiment 5, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
20 parts of 200 parts of sodium alginate, 45 parts of nano zine oxide, 25 parts of chitosan oligosaccharide and amido sodium lignin sulfonate；It is described to receive
Rice zinc oxide is nano zine oxide prepared by embodiment 1, and the amido sodium lignin sulfonate is that amido prepared by embodiment 2 is wooden
Plain sodium sulfonate.
Preparation method is similar to Example 3.
Comparative example 1, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and amido sodium lignin sulfonate；It is described to receive
Rice zinc oxide is commercially available nano zine oxide (being purchased from Beijing Deco Dao Jin Science and Technology Ltd.s, ZnO 30nm 99.9%), the amine
Base sodium lignin sulfonate is amido sodium lignin sulfonate prepared by embodiment 2.
Preparation method is similar to Example 3.
Comparative example 2, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan and amido sodium lignin sulfonate；It is described to receive
Rice zinc oxide is nano zine oxide prepared by embodiment 1, and the amido sodium lignin sulfonate is that amido prepared by embodiment 2 is wooden
Plain sodium sulfonate.
Preparation method is similar to Example 3.
Difference with embodiment 4 is：Chitosan oligosaccharide is replaced with into chitosan.
Comparative example 3, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 15
Part；The nano zine oxide is nano zine oxide prepared by embodiment 1.
Preparation method is similar to Example 3.
Difference with embodiment 4 is：Amido sodium lignin sulfonate is replaced with into the copolymerization of acrylic acid-acrylic acid hydroxypropyl acrylate
Thing.
Comparative example 4, a kind of filter membrane for filtrating leukocytes
The filter membrane for filtrating leukocytes is made up of following preparing raw material and its parts by weight：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and sodium lignin sulfonate；The nano oxygen
It is nano zine oxide prepared by embodiment 1 to change zinc.
Preparation method is similar to Example 3.
Difference with embodiment 4 is：Amido sodium lignin sulfonate is replaced with into sodium lignin sulfonate.
Test example one, the cell for the filter membrane of filtrating leukocytes filter effect test
1st, test material：Embodiment 3, embodiment 4, embodiment 5, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 are made
The standby filter membrane for filtrating leukocytes.
2nd, test method：
It is used for prepared by embodiment 3, embodiment 4, embodiment 5, comparative example 1, comparative example 2, comparative example 3 and comparative example 4
The filter membrane of filtrating leukocytes carries out on-line filtration to 400ml whole bloods, and evaluation leukocyte filter membranes remove leucocyte-removing and hematoblastic effect
Fruit, leucocyte and platelet counts in forward and backward whole blood are filtered using blood-counter system measure, measures mistake in 400ml whole bloods
The quantity for filtering proleukocyte is 2.5 × 109, the quantity for filtering thromboblast is 8 × 1010。
The assay method of wherein remaining leukocyte count is：By in the test tube of the whole blood sampling after filtering to polyethylene, with
After acridine orange liquid dyes the leucocyte spilt, it is measured with fluorescence microscope.The white blood cell concentration of measure is multiplied by recovery
Whole blood liquid measure, measure in collection bag contained remaining leukocyte count.
Leucocyte (blood platelet) rejection rate (%)=L0-L1/L0× 100, wherein L0To contain before filtering in unit volume whole blood
Some leucocyte (blood platelet) quantity, L1For leucocyte (blood platelet) quantity contained in unit volume whole blood after filtering.
3rd, result of the test：
Result of the test is as shown in table 1.
Table 1 is used for the filter membrane of filtrating leukocytes to leucocyte and hematoblastic filtration result
As shown in Table 1, the filter membrane for filtrating leukocytes that prepared by the embodiment of the present invention 3~5 is to the leucocyte in whole blood
It is respectively provided with blood platelet and preferably filters out efficiency, wherein being up to 99.999% to the rejection rate of leucocyte, is filtered out to hematoblastic
Rate is significantly better than the filter membrane for filtrating leukocytes of example 1-4 preparations by contrast to the leucocyte in whole blood up to more than 94%
With hematoblastic rejection rate, its filtration result has exceeded the requirement of the 3rd generation leucocyte filter.
Test example two, the filter opening plugging performance for the filter membrane of filtrating leukocytes detect experiment
1st, test material：Embodiment 3, embodiment 4, embodiment 5, comparative example 1, comparative example 2, comparative example 3 and comparative example 4 are made
The standby filter membrane for filtrating leukocytes.
2nd, test method：
The use prepared using embodiment 3, embodiment 4, embodiment 5, comparative example 1, comparative example 2, comparative example 3 and comparative example 4
In filtrating leukocytes filter membrane in the blood warehousing of storehouse the time it is longer, the high shear viscosity of blood is 4.8mPa.s after testing, in cut
Viscosity is 5.3mPa.s, low shear viscosity 10.5mPa.s, HCT 0.43%, and the abnormal blood that AI 2.34, IR are 0.53 is
Sample is detected, blood sample sampling 400ml, records each blood mistake with 400ml normal healths blood sample as a control group
Filter the time.
3rd, result of the test：
Result of the test is as shown in table 2.
Filtration time of the filter membrane of table 2 to whole blood
As shown in Table 2, the filter membrane for filtrating leukocytes being prepared using the embodiment of the present invention 3~4 is complete to 400mL
Blood, which filter, only needs 14~16min, illustrates the filter membrane for filtrating leukocytes of the offer of the present invention and has preferably parent
Water wettability, be advantageous to the filtering of blood.Meanwhile be 15~17min for the filtration time of the blood sample of exception, with filtering
The time of normal blood is held essentially constant, and the membrane filtration for filtrating leukocytes that comparative example 1~4 is prepared is abnormal
Blood sample at least extends the time of more than half, or even the phenomenon that can not be filtered occurs, illustrate provided by the present invention for
Self-control nano zine oxide, chitosan oligosaccharide in the filter membrane of filtrating leukocytes and the interaction of amination amido sodium lignin sulfonate can be to prevent
Only the aperture of filter membrane blocks, and alleviates blood clotting phenomenon, can effectively expand the use range of leukocyte filter membranes.
Claims (9)
1. a kind of filter membrane for filtrating leukocytes, it is characterised in that including following preparing raw material and its parts by weight：Alginic acid
10~20 parts of 100~200 parts of sodium, 35~45 parts of nano zine oxide, 15~25 parts of chitosan oligosaccharide and amido sodium lignin sulfonate；
The preparation method of the amido sodium lignin sulfonate is：
A. it is 65~75% lignin sulfonic acid sodium water solutions by the sodium lignin sulfonate mass percent concentration soluble in water that is configured to,
60~80 DEG C are heated to, adds triethylene tetramine, the glutaraldehyde water solution that mass percent concentration is 50% is then added dropwise, it is described
The drop rate of glutaraldehyde water solution is 0.3~0.5mL/min, and time for adding is controlled in 20~30min, is 80~90 in temperature
DEG C reaction 2~3h, obtain amido lignin sulfonic acid sodium solution；
B. the amido lignin sulfonic acid sodium solution that step A is obtained is stripped with absolute ethyl alcohol, obtains extract, washed with petroleum ether
Extract is washed, is freeze-dried, produced after washing.
2. it is used for the filter membrane of filtrating leukocytes as claimed in claim 1, it is characterised in that including following preparing raw material and its again
Measure number：
15 parts of 160 parts of sodium alginate, 38 parts of nano zine oxide, 22 parts of chitosan oligosaccharide and amido sodium lignin sulfonate.
3. it is used for the filter membrane of filtrating leukocytes as claimed in claim 1, it is characterised in that the preparation side of the nano zine oxide
Method is：
A. weighing zinc nitrate to add in deionized water, stir to being completely dissolved, be subsequently added into sodium carbonate, stirring is complete to reaction,
Centrifugation, obtains white depositions；
B. lauryl glucosyl is added in the white depositions obtained toward step a, 5~8min is stirred, is subsequently added into three hydroxyls
Trimethacrylate, 10~20min is stirred, obtains mixed liquor；
C. the obtained mixed liquors of step b are heated to 130~140 DEG C, insulation stands 30~40min, is then passed through nitrogen, continues
250~300 DEG C are heated to, insulation stands 40~60min；
D. the obtained mixed liquors of step c are cooled to 35~45 DEG C, add absolute ethyl alcohol, centrifuge to obtain sediment, then with anhydrous second
Alcohol washs, and centrifuges, and dries, and obtains the nano zine oxide that particle diameter is 8~10nm.
4. it is used for the filter membrane of filtrating leukocytes as claimed in claim 3, it is characterised in that zinc nitrate and carbon in the step a
The weight ratio of sour sodium is (1.2~1.6):1.
5. it is used for the filter membrane of filtrating leukocytes as claimed in claim 3, it is characterised in that white depositions in the step b
Solid-to-liquid ratio with lauryl glucosyl is 1g：(10~12) ml.
6. it is used for the filter membrane of filtrating leukocytes as claimed in claim 3, it is characterised in that white depositions in the step b
Solid-to-liquid ratio with trimethylol-propane trimethacrylate is 1g：(6~8) ml.
7. it is used for the filter membrane of filtrating leukocytes as claimed in claim 1, it is characterised in that the triethylene four in the step A
The addition of amine is the 20~30% of lignin sulfonic acid sodium water solution cumulative volume.
8. it is used for the filter membrane of filtrating leukocytes as claimed in claim 1, it is characterised in that be freeze-dried in the step B
Condition is：- 8 DEG C of 2~4h of pre-freeze, it is then 0.04~0.06mPa in vacuum, temperature is -18 DEG C of 20~24h of freezing.
9. the preparation method of the filter membrane for filtrating leukocytes as described in claim 1-8 is any, it is characterised in that including with
Lower step：
Sodium alginate is added to the water by S1, stirs to being completely dissolved, obtains sodium alginate aqueous solution；
Chitosan oligosaccharide and amido sodium lignin sulfonate are added to the water by S2, stir to being completely dissolved, obtain mixed liquor；
Nano zine oxide is added to the water by S3, stirs to being completely dissolved, obtains nano oxidized zinc solution；
S4 mixes the mixed liquor that step S2 is obtained with the obtained sodium alginate aqueous solutions of step S1, stirs, it is molten to obtain spinning
Liquid, the spinneret orifice for being then 0.08mm by aperture with measuring pump are squeezed into the nano oxidized zinc solution that step S3 is obtained, drawing-off,
With 95% Ethanol Treatment fiber, centrifugation sloughs drying fibrous, cutting after ethanol, combing, networking, the net that aperture is 4~3 μm is made
Shape structure, is produced.
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| US5783094A (en) * | 1995-04-13 | 1998-07-21 | Teva Medical Ltd. | Whole blood and platelet leukocyte filtration method |
| CN106139935A (en) * | 2016-06-23 | 2016-11-23 | 广州新克力生物科技有限公司 | A kind of leucocyte and hematoblastic filter membrane and preparation method thereof |
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| EP1452193B1 (en) * | 2001-12-03 | 2009-02-18 | Asahi Kasei Medical Co., Ltd. | Filter for selectively eliminating leukocytes |
| CN100376247C (en) * | 2004-10-14 | 2008-03-26 | 刘建平 | Preparation of bio-membrane in use for promoting wound healing, and preparation method |
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| CN104928799A (en) * | 2015-06-11 | 2015-09-23 | 青岛大学 | Preparation method for sustainable antibacterial nano-zinc oxide alginate fibers |
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| US5783094A (en) * | 1995-04-13 | 1998-07-21 | Teva Medical Ltd. | Whole blood and platelet leukocyte filtration method |
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