CN107211964A - Selenium-enriched earthworm cultural method - Google Patents
Selenium-enriched earthworm cultural method Download PDFInfo
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- CN107211964A CN107211964A CN201710371240.6A CN201710371240A CN107211964A CN 107211964 A CN107211964 A CN 107211964A CN 201710371240 A CN201710371240 A CN 201710371240A CN 107211964 A CN107211964 A CN 107211964A
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- 241000361919 Metaphire sieboldi Species 0.000 title claims abstract description 87
- 239000011669 selenium Substances 0.000 title claims abstract description 61
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 61
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims description 11
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- 239000011781 sodium selenite Substances 0.000 claims abstract description 27
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- 235000013305 food Nutrition 0.000 claims abstract description 23
- 238000002360 preparation method Methods 0.000 claims abstract description 17
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- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 15
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- 235000009566 rice Nutrition 0.000 claims description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/30—Rearing or breeding invertebrates
- A01K67/33—Earthworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
本发明涉及一种富硒蚯蚓的养殖方法,其包括以下步骤:1)秸秆处理:将秸秆粉碎,与氨水一起置于密闭装置中,进行微波软化,软化结束,获得软化秸秆;2)养殖基质的准备:将动物粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,进行发酵腐熟,腐熟结束加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔5~10天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45~50天;4)蚯蚓采收。本发明的富硒蚯蚓的养殖方法具有生长速度快,产量高及富硒效果好等优点。The invention relates to a breeding method for selenium-enriched earthworms, which comprises the following steps: 1) Straw treatment: crushing the straw, placing it in a closed device together with ammonia water, performing microwave softening, and obtaining softened straw after softening; 2) Culture substrate Preparation: mix animal feces, softened straw, bacteria agent and sodium selenite in proportion, carry out fermentation and decompose, add earthworm food attractant after decomposing, mix well, and obtain earthworm breeding substrate; 3) Feeding management: earthworm breeding substrate is flat Lay it on the breeding bed, spray the attractant on the surface, add earthworm seedlings, and carry out cultivation to maintain the water content of the earthworm breeding substrate, add the same earthworm breeding substrate every 5 to 10 days and spray the attractant on the surface, Breeding for 45-50 days; 4) earthworm harvesting. The cultivation method of selenium-enriched earthworms of the present invention has the advantages of fast growth, high yield, good selenium-enrichment effect, and the like.
Description
技术领域technical field
本发明涉及一种蚯蚓的养殖方法,特别涉及一种富硒蚯蚓的养殖方法。The invention relates to a method for cultivating earthworms, in particular to a method for cultivating selenium-enriched earthworms.
背景技术Background technique
硒是人和动物必须的微量元素,其可促进动物的生长,发育、提高免疫力及降低死亡率,但是我国属于严重缺硒的国家,从东北三省起斜穿至云贵高原,占中国国土面积的72%地区存在一条低硒地带,其中30%为严重缺硒地区,粮食等天然食物硒含量较低。华北、东北、西北等大中城市都属于缺硒地区。中国二十二个省市的部分地区,约七亿人生活在低硒地区,大部分地区食物中硒含量在0.02mg/kg以下,东南沿海是中国富硒地区,也只有0.10mg/kg,是联合国卫生组织规定的最低限。中国人群食物中尚达不到每天每人 40微克以上的标准补硒水平,尤其是缺硒地区人群,更难从食物中达到补硒的目的。Selenium is an essential trace element for humans and animals. It can promote the growth and development of animals, improve immunity and reduce mortality. However, my country is a country seriously deficient in selenium. There is a low-selenium zone in 72% of the regions, 30% of which are severe selenium-deficient regions, and natural foods such as grains have low selenium content. Large and medium-sized cities such as North China, Northeast China, and Northwest China are all selenium-deficient areas. In some areas of 22 provinces and cities in China, about 700 million people live in low-selenium areas, and the selenium content in food in most areas is below 0.02mg/kg. The southeast coast is China's selenium-rich areas, which are only 0.10mg/kg. It is the minimum limit stipulated by the United Nations Health Organization. The standard level of selenium supplementation of more than 40 micrograms per person per day is not yet reached in the food of the Chinese population, especially for people in selenium-deficient areas, it is even more difficult to achieve the purpose of selenium supplementation from food.
蚯蚓含有大量蛋白质、种类丰富的氨基酸、维生素及矿物质元素等营养物质,是一种很好的动物蛋白饲料。传统的养殖方法直接将无机硒添加到蚯蚓的养殖基质中,虽然蚯蚓对无机硒起到了一定的富集作用,但无机硒对蚯蚓的毒副作用亦是明显的,蚯蚓的存活率随着养殖基质中无机硒含量的提高而降低,造成了蚯蚓富硒效果的不佳。Earthworms contain a large amount of protein, a variety of amino acids, vitamins and mineral elements and other nutrients, and are a good animal protein feed. The traditional breeding method directly adds inorganic selenium to the culture medium of earthworms. Although earthworms have a certain enrichment effect on inorganic selenium, the toxic and side effects of inorganic selenium on earthworms are also obvious. The survival rate of earthworms increases with the growth of the culture medium. The increase of the content of inorganic selenium in the soil decreased, resulting in the poor selenium enrichment effect of earthworms.
发明内容Contents of the invention
本发明要解决的技术问题是提供一种蚯蚓生长快速及高效富集有机硒的蚯蚓养殖方法。The technical problem to be solved by the present invention is to provide an earthworm breeding method for rapid growth of earthworms and high-efficiency enrichment of organic selenium.
为了解决上述技术问题,本发明是通过以下技术方案实现的:一种富硒蚯蚓的养殖方法,其包括以下步骤:In order to solve the above technical problems, the present invention is achieved through the following technical solutions: a method for cultivating selenium-enriched earthworms, which comprises the following steps:
1)秸秆处理:将秸秆粉碎,与氨水一起置于密闭装置中,进行微波软化,软化结束,获得软化秸秆;1) Straw treatment: crush the straw, put it in a closed device together with ammonia water, and perform microwave softening. After the softening is completed, the softened straw is obtained;
2)养殖基质的准备:将动物粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,进行发酵腐熟,腐熟结束后加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mixing animal feces, softened straw, bacterial agent and sodium selenite in proportion, fermenting and decomposing, adding earthworm attractant after decomposing, and mixing well to obtain earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔5~10天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45~50天;3) Feeding and management: spread the earthworm breeding substrate on the breeding bed, spray the attractant on the surface, add earthworm seedlings, and carry out breeding to maintain the water content of the earthworm breeding substrate, and add the same earthworm breeding every 5 to 10 days Substrate and spray attractant on the surface, culture for 45-50 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3~5毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3-5 mm, put a collection container under the basket, and place them under the light. Next, the earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
优选的是,所述的秸秆为玉米秸秆、小麦秸秆、水稻秸秆中的一种或几种,秸秆粉碎的大小为0.01~1cm,所述的氨水的质量分数为10~20%,秸秆与氨水的质量比为10:(3~ 5),所述的微波软化的时间为5~10h,温度为110~150℃。Preferably, the straw is one or more of corn straw, wheat straw, and rice straw, the size of the crushed straw is 0.01-1 cm, the mass fraction of the ammonia water is 10-20%, and the straw and ammonia water The mass ratio is 10:(3-5), the microwave softening time is 5-10 hours, and the temperature is 110-150°C.
优选的是,所述的动物粪便为猪粪、牛粪、鸡粪中的一种或多种,所述的动物粪便、软化秸秆、菌剂与亚硒酸钠各组分的质量比为(40~50):(30~50):(1~3):(0.05~0.1)。Preferably, described animal manure is one or more in pig manure, cow manure, chicken manure, and the mass ratio of described animal manure, softening stalk, bacterial agent and each component of sodium selenite is ( 40-50): (30-50): (1-3): (0.05-0.1).
优选的是,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分的质量百分比数为酵母菌15~25%、乳酸菌10~15%、枯草芽孢杆菌10~15%、光合细菌5~10%、放线菌10~15%、绿色木霉10~15%及白腐真菌25~35%。Preferably, the bacterial agent is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white-rot fungus, wherein the mass percentage of each component in the bacterial agent is 15-15% of yeast 25%, lactic acid bacteria 10-15%, Bacillus subtilis 10-15%, photosynthetic bacteria 5-10%, actinomycetes 10-15%, Trichoderma viride 10-15% and white rot fungi 25-35%.
优选的是,所述的发酵腐熟的时间为6~8天,所述的诱食剂的添加量为养殖基质总质量的2~5%。Preferably, the fermentation time is 6-8 days, and the added amount of the food attractant is 2-5% of the total mass of the culture substrate.
优选的是,所述的诱食剂的制备方法为将1~2Kg的腐烂瓜果磨成浆液,加入亚硒酸钠10~20mg、糖蜜100~200g、微晶纤维素5~10g及橘皮粉10~20g混合均匀获得。Preferably, the preparation method of the food attractant is to grind 1-2Kg of rotten melons and fruits into slurry, add 10-20 mg of sodium selenite, 100-200 g of molasses, 5-10 g of microcrystalline cellulose and orange peel Mix 10-20g powder evenly to get it.
优选的是,所述的腐烂瓜果为冬瓜、苦瓜、木瓜、苹果、香蕉、梨、菠萝、西瓜、香瓜、哈密瓜、芒果中的一种或几种。Preferably, the rotten fruits are one or more of wax gourd, bitter gourd, papaya, apple, banana, pear, pineapple, watermelon, muskmelon, cantaloupe and mango.
优选的是,所述的蚯蚓种苗为赤子爱胜蚯蚓,所述的蚯蚓养殖基质的含水量为50~ 70%。Preferably, the earthworm seedlings are Eisenia chinensis earthworms, and the water content of the earthworm breeding substrate is 50-70%.
本发明的优点:本发明方法的秸秆软化方法,具有软化时间短,软化效果好特点,能提高秸秆的利用率;在秸秆与动物粪便中加入亚硒酸钠一起发酵腐熟,亚硒酸钠经微生物作用转换为有机硒,降低了硒元素对蚯蚓的毒性,提高了蚯蚓富硒的能力,使蚯蚓的富硒能力提高了300%以上,秸秆经过微生物的分解作用,不仅增加了菌蛋白含量,秸秆的利用率也提高5%以上;在养殖基质中加入诱食剂,能增加蚯蚓的摄食欲望,增强其消化能力,促进其生长,使蚯蚓的产量提高15%以上。Advantages of the present invention: the straw softening method of the present invention has the characteristics of short softening time and good softening effect, and can improve the utilization rate of straw; adding sodium selenite to straw and animal manure to ferment and decompose together, sodium selenite is The conversion of microorganisms to organic selenium reduces the toxicity of selenium to earthworms, improves the selenium-enrichment ability of earthworms, and increases the selenium-enrichment ability of earthworms by more than 300%. The straw is decomposed by microorganisms, which not only increases the content of mycoprotein, The utilization rate of straw is also increased by more than 5%; adding food attractant to the culture medium can increase the feeding desire of earthworms, enhance their digestion ability, promote their growth, and increase the output of earthworms by more than 15%.
具体实施方式detailed description
本发明的其它优点、目标和特征将部分通过下面的说明体现,部分还将通过对本发明的研究和实践而为本领域的技术人员所理解。Other advantages, objectives and features of the present invention will partly be embodied through the following descriptions, and partly will be understood by those skilled in the art through the study and practice of the present invention.
下述实施例用于进一步说明但不限于本发明。The following examples are provided to further illustrate but not limit the present invention.
实施例1本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 1 A kind of realization form of the cultivation method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将秸秆粉碎,与氨水一起置于密闭装置中,进行微波软化,软化结束,获得软化秸秆;1) Straw treatment: crush the straw, put it in a closed device together with ammonia water, and perform microwave softening. After the softening is completed, the softened straw is obtained;
2)养殖基质的准备:将动物粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,进行发酵腐熟,腐熟结束后加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mixing animal feces, softened straw, bacterial agent and sodium selenite in proportion, fermenting and decomposing, adding earthworm attractant after decomposing, and mixing well to obtain earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行蚯蚓养殖,保持蚯蚓养殖基质的含水量,每隔5天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖50天;3) Feeding and management: spread the earthworm breeding substrate on the breeding bed, spray the attractant on the surface, add earthworm seedlings, carry out earthworm breeding, keep the water content of the earthworm breeding substrate, and add the same earthworm breeding substrate every 5 days And spray attractant on the surface, breed for 50 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径5毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 5 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
秸秆氨化软化所需时间较长,在氨化软化的过程中加入微波的作用,能有效的减少软化的时间,提高软化的效率,同时微波的作用也使秸秆的软化效果更好,是微生物或蚯蚓分泌的酶更容易作用于纤维素;秸秆经软化后与动物粪便一起发酵,能提高秸秆的降解率;在腐熟的过程中加入亚硒酸钠,能使无机的亚硒酸钠转化为有机硒,降低无机硒对蚯蚓的毒副作用,也使得蚯蚓富集硒的效果更理想,与单独使用无机硒的富集效果相比,使用有机硒蚯蚓的富硒效果高3倍以上。The ammoniation and softening of straw takes a long time. Adding the role of microwave in the process of ammoniation and softening can effectively reduce the softening time and improve the softening efficiency. At the same time, the effect of microwave can also make the softening effect of straw better. Or the enzymes secreted by earthworms are more likely to act on cellulose; the straw is softened and fermented with animal manure, which can increase the degradation rate of the straw; adding sodium selenite during the decomposing process can convert inorganic sodium selenite into Organic selenium reduces the toxic and side effects of inorganic selenium on earthworms, and also makes the selenium enrichment effect of earthworms more ideal. Compared with the enrichment effect of using inorganic selenium alone, the selenium enrichment effect of earthworms using organic selenium is more than 3 times higher.
实施例2本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 2 A kind of realization form of the cultivation method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为1cm,与质量分数为20%的氨水30kg 一起置于密闭装置中,使用微波加热,于110℃软化10h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 1 cm, place them in a closed device together with 30 kg of ammonia water with a mass fraction of 20%, heat them with microwaves, soften them at 110° C. for 10 hours, and obtain softened straws after softening is completed;
2)养殖基质的准备:将动物粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,进行发酵腐熟,腐熟结束后加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mixing animal feces, softened straw, bacterial agent and sodium selenite in proportion, fermenting and decomposing, adding earthworm attractant after decomposing, and mixing well to obtain earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔5天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 5 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
在微波的加热过程中,秸秆中易分解成分(如纤维素、半纤维素分解)部分分解,使氨气更易与有机物质发生氨解反应,通过氨解反应,破坏木质素与多糖间酯键,削弱木质素与纤维素之间的联系,使秸秆更容易被微生物分解。During the microwave heating process, the easy-to-decompose components in the straw (such as cellulose and hemicellulose decomposition) are partially decomposed, making it easier for ammonia gas to undergo ammonolysis reactions with organic substances. Through the ammonolysis reaction, the ester bond between lignin and polysaccharides is destroyed. , Weaken the link between lignin and cellulose, making the straw easier to be decomposed by microorganisms.
实施例3本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 3 A kind of realization form of the cultivation method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为1cm,与质量分数为10%的氨水50kg 一起置于密闭装置中,使用微波加热,150℃软化5h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 1 cm, place them in a closed device together with 50 kg of ammonia water with a mass fraction of 10%, heat them with microwaves, soften them at 150°C for 5 hours, and obtain softened straws after softening;
2)养殖基质的准备:将猪粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,猪粪便、软化秸秆、菌剂与亚硒酸钠混合物各组分的质量比为50:30:1:0.1进行发酵腐熟,腐熟结束加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mix pig manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of the mixture of pig manure, softened straw, bacterial agent and sodium selenite is 50:30: 1:0.1 for fermentation and decomposing, after decomposing, add earthworm attractant, mix well, and obtain earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔10天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 10 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
实施例4本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 4 A kind of realization form of the breeding method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为0.5cm,与质量分数为10%的氨水50kg 一起置于密闭装置中,使用微波加热,150℃软化5h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 0.5 cm, place them in a closed device together with 50 kg of ammonia water with a mass fraction of 10%, heat them with microwaves, soften them at 150°C for 5 hours, and obtain softened straws after softening;
2)养殖基质的准备:将牛粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,牛粪便、软化秸秆、菌剂与亚硒酸钠各组分的质量比为50:30:1:0.1,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分所占质量百分比数为酵母菌15%、乳酸菌10%、枯草芽孢杆菌10%、光合细菌10%、放线菌10%、绿色木霉15%及白腐真菌30%,进行发酵腐熟,腐熟结束加入蚯蚓诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mix cow manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of cow manure, softened straw, bacterial agent and sodium selenite is 50:30:1 : 0.1, the inoculum is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white-rot fungus, wherein the mass percentage of each component in the inoculum is 15% of yeast , 10% of lactic acid bacteria, 10% of Bacillus subtilis, 10% of photosynthetic bacteria, 10% of actinomycetes, 15% of Trichoderma viride and 30% of white rot fungus, ferment and decompose, add earthworm food attractant after decomposing, mix well, and obtain Earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔10天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 10 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
加入菌剂能对秸秆进行有效的分解作用,使秸秆的碳水化合物转化为菌蛋白,也使蚯蚓对秸秆的消化吸收更加的充分,提高秸秆的利用率。The addition of bacterial agents can effectively decompose the straw, convert the carbohydrates of the straw into mycoprotein, and make the digestion and absorption of the straw more fully by earthworms, improving the utilization rate of the straw.
实施例5本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 5 A kind of realization form of the breeding method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为0.5cm,与质量分数为15%的氨水45kg 一起,置于密闭装置中,使用微波加热,130℃软化7h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 0.5 cm, place them in a closed device together with 45 kg of ammonia water with a mass fraction of 15%, heat them with microwaves, soften them at 130°C for 7 hours, and obtain softened straws after softening is complete;
2)养殖基质的准备:将牛粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,牛粪便、软化秸秆、菌剂与亚硒酸钠各组分的质量比为50:30:1:0.1,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分所占质量百分比数为酵母菌15%、乳酸菌10%、枯草芽孢杆菌10%、光合细菌10%、放线菌10%、绿色木霉15%及白腐真菌30%,进行发酵腐熟6天,腐熟结束加入蚯蚓养殖基质总质量2%的诱食剂,混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mix cow manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of cow manure, softened straw, bacterial agent and sodium selenite is 50:30:1 : 0.1, the inoculum is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white-rot fungus, wherein the mass percentage of each component in the inoculum is 15% of yeast , 10% of lactic acid bacteria, 10% of Bacillus subtilis, 10% of photosynthetic bacteria, 10% of actinomycetes, 15% of Trichoderma viride and 30% of white rot fungi, fermented and decomposed for 6 days, and added 2% of the total mass of earthworm breeding substrate after decomposing The food attractant, mix, obtain the earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔10天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 10 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
增加诱食剂能增加蚯蚓的摄食欲望,促进蚯蚓的生长,使蚯蚓的产量提高10%以上。Increasing the food attractant can increase the feeding desire of earthworms, promote the growth of earthworms, and increase the output of earthworms by more than 10%.
实施例6本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 6 A kind of realization form of the breeding method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为0.5cm,与质量分数为15%的氨水45kg 一起置于密闭装置中,使用微波加热,130℃软化7h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 0.5 cm, place them in a closed device together with 45 kg of ammonia water with a mass fraction of 15%, heat them with microwaves, soften them at 130°C for 7 hours, and obtain softened straws after softening;
2)养殖基质的准备:将牛粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,牛粪便、软化秸秆、菌剂与亚硒酸钠混合物各组分的质量比为50:30:1:0.1,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分所占质量百分比数为酵母菌15%、乳酸菌10%、枯草芽孢杆菌10%、光合细菌10%、放线菌 10%、绿色木霉15%及白腐真菌30%,进行发酵腐熟6天,腐熟结束加入蚯蚓养殖基质总质量的2%的诱食剂,所述的诱食剂的制备方法为将1Kg的腐烂瓜果磨成浆液,加入亚硒酸钠10mg、糖蜜100g、微晶纤维素10g及橘皮粉10g,蚯蚓养殖基质与诱食剂混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: Mix cow manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of the mixture of cow manure, softened straw, bacterial agent and sodium selenite is 50:30: 1:0.1, the bacterial agent is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white rot fungus, wherein the mass percentage of each component in the bacterial agent is yeast 15 %, 10% of lactic acid bacteria, 10% of Bacillus subtilis, 10% of photosynthetic bacteria, 10% of actinomycetes, 15% of Trichoderma viride and 30% of white rot fungi, fermented and decomposed for 6 days, and added 10% of the total mass of earthworm breeding substrate after decomposing 2% food attractant, the preparation method of described food attractant is to grind the rotten melon and fruit of 1Kg into slurry, add sodium selenite 10mg, molasses 100g, microcrystalline cellulose 10g and orange peel powder 10g, earthworm culture The substrate is mixed with the food attractant to obtain the earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔7天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 7 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
通过加入糖蜜能增加蚯蚓的摄食欲望,促进蚯蚓的生长;通过加入微晶纤维能增加蚯蚓体内纤维素酶的分泌,提高秸秆的消化率;通过加入橘皮粉能刺激蚯蚓内腔,提高消化率。Adding molasses can increase the feeding desire of earthworms and promote the growth of earthworms; adding microcrystalline fiber can increase the secretion of cellulase in earthworms and improve the digestibility of straw; adding orange peel powder can stimulate the inner cavity of earthworms and improve digestibility .
实施例7本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 7 A kind of realization form of the cultivation method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为0.5cm,与质量分数为15%的氨水45kg 一起置于密闭装置中,使用微波加热,130℃软化7h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 0.5 cm, place them in a closed device together with 45 kg of ammonia water with a mass fraction of 15%, heat them with microwaves, soften them at 130°C for 7 hours, and obtain softened straws after softening;
2)养殖基质的准备:将牛粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,牛粪便、软化秸秆、菌剂与亚硒酸钠各组分的质量比为50:30:1:0.1,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分所占质量百分比数为酵母菌15%、乳酸菌10%、枯草芽孢杆菌10%、光合细菌10%、放线菌10%、绿色木霉15%及白腐真菌30%,进行发酵腐熟6天,腐熟结束加入蚯蚓养殖基质总质量的 2%的诱食剂,所述的诱食剂的制备方法为将1Kg的腐烂苹果磨成浆液,加入亚硒酸钠10mg、糖蜜100g、微晶纤维素10g及橘皮粉10g,蚯蚓养殖基质与诱食剂混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mix cow manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of cow manure, softened straw, bacterial agent and sodium selenite is 50:30:1 : 0.1, the bacterial agent is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white rot fungus, wherein the mass percentage of each component in the bacterial agent is 15% of yeast , 10% of lactic acid bacteria, 10% of Bacillus subtilis, 10% of photosynthetic bacteria, 10% of actinomycetes, 15% of Trichoderma viride and 30% of white rot fungi, fermented and decomposed for 6 days, and added 2% of the total mass of earthworm breeding substrate after decomposing. % food attractant, the preparation method of described food attractant is to grind the rotten apple of 1Kg into slurry, add sodium selenite 10mg, molasses 100g, microcrystalline cellulose 10g and orange peel powder 10g, earthworm breeding substrate and The food attractant is mixed evenly to obtain the earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量,每隔7天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖45天;3) Feeding and management: the earthworm breeding substrate is laid flat on the breeding bed, and the surface is sprayed with an attractant, and the earthworm seedlings are added for cultivation to keep the water content of the earthworm breeding substrate, and the same earthworm breeding substrate is added every 7 days. Spray feed-attractant on the surface, breed for 45 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
实施例8本发明的一种富硒蚯蚓的养殖方法的一种实现形式,其包括以下步骤:Embodiment 8 A kind of realization form of the cultivation method of a kind of selenium-enriched earthworm of the present invention, it comprises the following steps:
1)秸秆处理:将100kg玉米秸秆粉碎,粉碎大小为0.5cm,与质量分数为15%的氨水45kg 一起置于密闭装置中,使用微波加热,130℃软化7h,软化结束,获得软化秸秆;1) Straw treatment: crush 100 kg of corn stalks to a size of 0.5 cm, place them in a closed device together with 45 kg of ammonia water with a mass fraction of 15%, heat them with microwaves, soften them at 130°C for 7 hours, and obtain softened straws after softening;
2)养殖基质的准备:将牛粪便、软化秸秆、菌剂与亚硒酸钠按比例混合,牛粪便、软化秸秆、菌剂与亚硒酸钠各组分的质量比为50:30:1:0.1,所述的菌剂为酵母菌、乳酸菌、枯草芽孢杆菌、光合细菌、放线菌、绿色木霉及白腐真菌,其中菌剂中各组分所占质量百分比数为酵母菌15%、乳酸菌10%、枯草芽孢杆菌10%、光合细菌10%、放线菌10%、绿色木霉15%及白腐真菌30%,进行发酵腐熟6天,腐熟结束加入蚯蚓养殖基质总质量2%的诱食剂,所述的诱食剂的制备方法为将1Kg的腐烂苹果磨成浆液,加入亚硒酸钠10mg、糖蜜100g、微晶纤维素10g及橘皮粉10g,蚯蚓养殖基质与诱食剂混匀,获得蚯蚓养殖基质;2) Preparation of breeding substrate: mix cow manure, softened straw, bacterial agent and sodium selenite in proportion, and the mass ratio of each component of cow manure, softened straw, bacterial agent and sodium selenite is 50:30:1 : 0.1, the inoculum is yeast, lactic acid bacteria, Bacillus subtilis, photosynthetic bacteria, actinomycetes, Trichoderma viride and white-rot fungus, wherein the mass percentage of each component in the inoculum is 15% of yeast , 10% of lactic acid bacteria, 10% of Bacillus subtilis, 10% of photosynthetic bacteria, 10% of actinomycetes, 15% of Trichoderma viride and 30% of white rot fungi, fermented and decomposed for 6 days, and added 2% of the total mass of earthworm breeding substrate after decomposing The food attractant, the preparation method of described food attractant is that the rotten apple of 1Kg is ground into slurry, adds sodium selenite 10mg, molasses 100g, microcrystalline cellulose 10g and orange peel powder 10g, earthworm culture substrate and lure The food preparation is mixed evenly to obtain the earthworm breeding substrate;
3)饲养管理:蚯蚓养殖基质平铺在养殖床上,并在表面喷洒上诱食剂,加入赤子爱胜蚯蚓种苗,进行养殖,保持蚯蚓养殖基质的含水量为50%,每隔7天添加相同的蚯蚓养殖基质并在表面喷洒诱食剂,养殖50天;3) Feeding and management: spread the earthworm breeding substrate on the breeding bed, spray the attractant on the surface, add the seedlings of the earthworm Eisensis, and carry out the breeding, keep the water content of the earthworm breeding substrate at 50%, and add it every 7 days The same earthworm breeding substrate and spray attractant on the surface, cultivated for 50 days;
4)蚯蚓采收:在光照下逐层刮去蚯蚓粪,驱使蚯蚓钻到养殖床下层,收集养殖床下层的蚯蚓,置于孔径3毫米的大筐上,筐下放收集容器,在光照下,蚯蚓自动钻入筛下容器,获得富硒蚯蚓。4) Harvesting of earthworms: Scrape off the vermicompost layer by layer under the light, drive the earthworms to drill into the lower layer of the culture bed, collect the earthworms in the lower layer of the culture bed, place them on a large basket with an aperture of 3 mm, put a collection container under the basket, and under the light, Earthworms automatically drill into the container under the sieve to obtain selenium-enriched earthworms.
尽管本发明的实施方案已公开如上,但其并不仅仅限于说明书和实施方式中所列运用,它完全可以被适用于各种适合本发明的领域,对于熟悉本领域的人员而言,可容易地实现另外的修改,因此在不背离权利要求及等同范围所限定的一般概念下,本发明并不限于特定的细节。Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the specification and implementation, it can be applied to various fields suitable for the present invention, and it can be easily understood by those skilled in the art Therefore, the invention is not limited to the specific details without departing from the general concept defined by the claims and their equivalents.
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