CN107202788B - Chinese medicine quality is quickly defined the level test paper and preparation method thereof - Google Patents
Chinese medicine quality is quickly defined the level test paper and preparation method thereof Download PDFInfo
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- 238000012360 testing method Methods 0.000 title claims abstract description 42
- 239000003814 drug Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 229910052785 arsenic Inorganic materials 0.000 claims abstract description 28
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 28
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 28
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229910052753 mercury Inorganic materials 0.000 claims abstract description 28
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 14
- 239000002904 solvent Substances 0.000 claims description 90
- 238000004090 dissolution Methods 0.000 claims description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 24
- 239000008363 phosphate buffer Substances 0.000 claims description 23
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- 235000019441 ethanol Nutrition 0.000 claims description 13
- SMMIDVLUFMPWFN-UHFFFAOYSA-N 4-nitro-n-[(4-phenyldiazenylphenyl)diazenyl]aniline Chemical compound C1=CC([N+](=O)[O-])=CC=C1NN=NC1=CC=C(N=NC=2C=CC=CC=2)C=C1 SMMIDVLUFMPWFN-UHFFFAOYSA-N 0.000 claims description 9
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 9
- 239000011609 ammonium molybdate Substances 0.000 claims description 9
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 9
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 9
- 229940010552 ammonium molybdate Drugs 0.000 claims description 9
- DWCZIOOZPIDHAB-UHFFFAOYSA-L methyl green Chemical compound [Cl-].[Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)[N+](C)(C)C)=C1C=CC(=[N+](C)C)C=C1 DWCZIOOZPIDHAB-UHFFFAOYSA-L 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 9
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 9
- 229940068984 polyvinyl alcohol Drugs 0.000 claims description 9
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 9
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 9
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- GDSOZVZXVXTJMI-SNAWJCMRSA-N (e)-1-methylbut-1-ene-1,2,4-tricarboxylic acid Chemical compound OC(=O)C(/C)=C(C(O)=O)\CCC(O)=O GDSOZVZXVXTJMI-SNAWJCMRSA-N 0.000 claims description 8
- 108010022752 Acetylcholinesterase Proteins 0.000 claims description 8
- 229920000936 Agarose Polymers 0.000 claims description 8
- 108010010803 Gelatin Proteins 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 8
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 8
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 8
- 229940022698 acetylcholinesterase Drugs 0.000 claims description 8
- 239000013078 crystal Substances 0.000 claims description 8
- 229920000159 gelatin Polymers 0.000 claims description 8
- 239000008273 gelatin Substances 0.000 claims description 8
- 235000019322 gelatine Nutrition 0.000 claims description 8
- 235000011852 gelatine desserts Nutrition 0.000 claims description 8
- WCJLIWFWHPOTAC-UHFFFAOYSA-N rhodizonic acid Chemical compound OC1=C(O)C(=O)C(=O)C(=O)C1=O WCJLIWFWHPOTAC-UHFFFAOYSA-N 0.000 claims description 8
- 239000011734 sodium Substances 0.000 claims description 8
- 229910052708 sodium Inorganic materials 0.000 claims description 8
- 239000001632 sodium acetate Substances 0.000 claims description 8
- 235000017281 sodium acetate Nutrition 0.000 claims description 8
- IDOQDZANRZQBTP-UHFFFAOYSA-N 2-[2-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=CC=C1OCCO IDOQDZANRZQBTP-UHFFFAOYSA-N 0.000 claims description 7
- 229920002565 Polyethylene Glycol 400 Polymers 0.000 claims description 7
- 229920004929 Triton X-114 Polymers 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 claims description 7
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 claims description 7
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims description 7
- 235000019345 sodium thiosulphate Nutrition 0.000 claims description 7
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 5
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- LMBWSYZSUOEYSN-UHFFFAOYSA-N diethyldithiocarbamic acid Chemical compound CCN(CC)C(S)=S LMBWSYZSUOEYSN-UHFFFAOYSA-N 0.000 claims description 3
- 229950004394 ditiocarb Drugs 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- ZKDNBOAOTQCXLM-UHFFFAOYSA-N 2,3-dihydroxybutanedioic acid;potassium;sodium Chemical compound [Na].[K].OC(=O)C(O)C(O)C(O)=O ZKDNBOAOTQCXLM-UHFFFAOYSA-N 0.000 claims 1
- 102100033639 Acetylcholinesterase Human genes 0.000 claims 1
- 102000003914 Cholinesterases Human genes 0.000 claims 1
- 108090000322 Cholinesterases Proteins 0.000 claims 1
- 125000002339 acetoacetyl group Chemical group O=C([*])C([H])([H])C(=O)C([H])([H])[H] 0.000 claims 1
- 229940048961 cholinesterase Drugs 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 29
- 241000411851 herbal medicine Species 0.000 abstract description 23
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 239000000447 pesticide residue Substances 0.000 abstract description 4
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 238000012549 training Methods 0.000 abstract description 2
- 230000000007 visual effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 64
- 230000000052 comparative effect Effects 0.000 description 10
- 239000000575 pesticide Substances 0.000 description 9
- 102000012440 Acetylcholinesterase Human genes 0.000 description 7
- 238000007654 immersion Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000012086 standard solution Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002604 ultrasonography Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002168 ethanoic acid esters Chemical class 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 235000008216 herbs Nutrition 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 239000003094 microcapsule Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- KSPIHGBHKVISFI-UHFFFAOYSA-N Diphenylcarbazide Chemical compound C=1C=CC=CC=1NNC(=O)NNC1=CC=CC=C1 KSPIHGBHKVISFI-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- SNDJGKIVHKOEHY-UHFFFAOYSA-M S(=S)(=O)(O)O.S[Na] Chemical compound S(=S)(=O)(O)O.S[Na] SNDJGKIVHKOEHY-UHFFFAOYSA-M 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DUEPRVBVGDRKAG-UHFFFAOYSA-N carbofuran Chemical compound CNC(=O)OC1=CC=CC2=C1OC(C)(C)C2 DUEPRVBVGDRKAG-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000011247 coating layer Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 125000004177 diethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- VIOCLHQLOPJBSR-UHFFFAOYSA-N dimethyl sulfate;phenol Chemical compound COS(=O)(=O)OC.OC1=CC=CC=C1 VIOCLHQLOPJBSR-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052752 metalloid Inorganic materials 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- UQJQVUOTMVCFHX-UHFFFAOYSA-L nabam Chemical compound [Na+].[Na+].[S-]C(=S)NCCNC([S-])=S UQJQVUOTMVCFHX-UHFFFAOYSA-L 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 239000003993 organochlorine pesticide Substances 0.000 description 1
- 239000003987 organophosphate pesticide Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
The invention belongs to sample detection technical fields, and in particular to Chinese medicine quality is quickly defined the level test paper and preparation method thereof.Test paper of the present invention is only made of substrate and developing paper two parts, and structurally and operationally all very simple, this test paper can be used for quickly detecting lead, cadmium, mercury, 4 kinds of elements of arsenic and pesticide residue content in Chinese herbal medicine in 0.5-1min；By visual observation you can get it accurate quantitative detection conclusion, it is not required to be shown with expensive instrument, it is easy to operate, it can be grasped without specialized training；Production cost is low, and accuracy is high, reproducible, is extremely suitable for the detection real-time, quickly of Chinese herbal medicine scene and monitoring.
Description
Technical field
Invention belongs to sample detection technical field, and in particular to Chinese medicine quality is quickly defined the level test paper and preparation method thereof.
Background technique
Heavy metal is a key factor for influencing Chinese medicine quality, and harmful heavy metal element refers mainly in Chinese medicine
Lead, cadmium, mercury, copper, chromium etc., arsenic are also one of harmful element in Chinese medicine as a kind of metalloid element, routinely monitor have lead,
Cadmium, mercury and four kinds of arsenic.
Pesticide (Pesticides) is primarily referred to as endangering harmful organism (such as pest, evil of agriculture forest and husbandry production for preventing and treating
Mite, nematode, pathogen, weeds and muroid etc.) and coordinate plant growth chemicals, mainly include organo-chlorine pesticide, organic
Several major class such as phosphorus pesticide, carbamate chemicals for agriculture.Wherein, organophosphorus pesticide, carbamate chemicals for agriculture can inhibit significantly
The activity of acetylcholinesterase, long-term intake can cause to damage to the nervous system of people and animals, thus by multinational disabling or limit the use of.
The control of Chinese medicine quality is all a problem to be solved all the time, in the quality control of Chinese medicine, is established fast
Speed, conveniently, accurate detection method it is significant.Test paper detection is a kind of quick detection means, currently used for Chinese medicine toxicity
Element and remaining detect of medicine predominantly stay in the detection of single-element, and a kind of test paper can only often detect a kind of substance, right
The entry detection of Chinese medicine product generally requires to prepare a variety of test paper either a variety of detection means simultaneously, in use not enough
It is convenient.
Summary of the invention
In place of the above the deficiencies in the prior art, the present invention provides a kind of test paper, and test paper of the present invention is by substrate and colour developing
Paper two parts composition, it is structurally and operationally all very simple, it can be simultaneously to lead, cadmium, mercury, 4 kinds of elements of arsenic and pesticide in Chinese herbal medicine
Residual is used for quickly detecting, and is extremely suitable for the detection real-time, quickly of Chinese herbal medicine scene and monitoring.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows:
Chinese medicine quality is quickly defined the level test paper, is made of 1 piece of rectangular substrate and 5 pieces of developing papers, the developing paper includes
Lead developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper, the residual developing paper of agriculture, the developing paper includes following processing step:
1) lead developing paper: 0.3-0.5g rhodizonic acid, 5-10g PEG-400, the bad hematic acid sodium of 3-5g are added to 50- by a.
In 70ml water, ultrasonic dissolution adjusts pH 1.5-2.5 to get solvent 1；B. filter paper is placed in solvent 1 and impregnates 30-60s, Yu Wen
Spend 65-70 DEG C of drying 30-40min；
2) cadmium developing paper: a. is by 0.3-0.5g potassium hydroxide, 12-18g PEG-400,0.1-0.3g cadion, 2.0-
3.0mL2.0% phenanthroline, 1-2g sodium thiosulfate, 1-2g sodium potassium tartrate tetrahydrate are added in 50-60ml ethyl alcohol, ultrasonic dissolution,
PH 6.5-7.5 is adjusted to get solvent 2；B. filter paper is placed in solvent 2 and is impregnated for 30-60s, in 65-70 DEG C of drying 30- of temperature
40min；
3) mercury developing paper: the Triton X-114 of 0.8-1.2g crystal violet, 5-10g polyvinylpyrrolidone, 3-5ml are added
Enter into 5%-10% ethanol water, ultrasonic dissolution, adjusts pH 8.5-9.5 to get solvent 3；Filter paper is placed in solvent 3
30-60s is impregnated, in 65-70 DEG C of temperature, drying 10-20min；
4) arsenic developing paper: take 1-3g ammonium molybdate, 0.2-0.5g citric acid, 0.05-0.1g agarose, 3-5mL methyl green molten
Liquid, 1-2mL sulfuric acid solution, 1-2mL poly-vinyl alcohol solution add water to be settled to 20mL, and 60-70 DEG C of heating 15-20min is to get molten
Agent 4；Filter paper is placed in solvent 4 and impregnates 1-5min, is cooled down at room temperature；
5) 0.2-0.4g thiocarbamide, 0.2-0.3g sodium acetate, 0.5-1.0g natrium adetate, 0.2- 1. the residual developing paper of agriculture: are taken
The phosphate buffer of 0.3g sodium diethyldithiocarbamate and 50mLpH=7.5, mixing, is added 0.02- while stirring
The acetylcholinesterase of 0.04g is added 0.1-0.5g gelatin, is allowed to be uniformly dispersed, the phosphate buffer of pH=7.5 is added i.e.
It obtains solvent and is settled to 100mL to get solvent 5；Filter paper is placed in solvent 5 and impregnates 1-5min, is cooled down at room temperature.
Preferably, the substrate front side side by side 5 sizes identical depth be 0.3-0.5cm, diameter 0.8-1.0cm
Cylinder shape groove, the groove is for placing the lead developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper and the residual colour developing of agriculture
Paper.
Preferably, the step 1) are as follows: 0.5g rhodizonic acid, 5g PEG-400, the bad hematic acid sodium of 3g are added to 70ml
In water, ultrasonic dissolution adjusts pH 2.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 60s, is dried in 65 DEG C of temperature
30min。
Preferably, the step 2) are as follows: by 0.3g potassium hydroxide, 18g PEG-400,0.3g cadion, 3.0mL2.0%
Phenanthroline, 1g sodium thiosulfate, 2g sodium potassium tartrate tetrahydrate are added in 60ml ethyl alcohol, ultrasonic dissolution, adjust pH 7.5 to get molten
Agent 2；Filter paper is placed in solvent 2 and is impregnated for 60s, dries 40min in temperature 70 C.
Preferably, the step 3) are as follows: add the TritonX-114 of 1.2g crystal violet, 5g polyvinylpyrrolidone, 5ml
Enter into 10% ethanol water, ultrasonic dissolution, adjusts pH 8.5 to get solvent 3；Filter paper is placed in solvent 3 and impregnates 60s,
In temperature 70 C, drying 20min.
Preferably, the step 4) are as follows: take 3g ammonium molybdate, 0.5g citric acid, 0.1g agarose, 5mL methyl green solution,
2mL sulfuric acid solution, 1mL poly-vinyl alcohol solution add water to be settled to 20mL, and 70 DEG C of heating 20min are to get solvent 4；Filter paper is placed in
5min is impregnated in solvent 4, is cooled down at room temperature；
Preferably, the step 5) are as follows: 1. take 0.2g thiocarbamide, 0.2g sodium acetate, 0.5g natrium adetate, 0.3g diethyl
The acetylcholine of 0.02g is added in the phosphate buffer of nabam and 50mLpH=7.5, mixing while stirring
Esterase is added 0.1g gelatin, is allowed to be uniformly dispersed, and the phosphate buffer that pH=7.5 is added is settled to 100mL up to solvent,
Up to solvent 5；Filter paper is placed in solvent 5 and impregnates 5min, is cooled down at room temperature.
Preferably, the step 5) further relates to developing solution preparation, preparation step are as follows: take 5-10ml, 0.2% indophenols acetic acid esters
Solution is added in the phosphate buffer of 50mL0.1mol/LpH=7.5.
Compared with prior art, the beneficial effects of the present invention are:
It is only made of substrate and developing paper two parts, structurally and operationally all very simple, this test paper can be in 0.5-1min
Lead, cadmium, mercury, 4 kinds of elements of arsenic and pesticide residue content in Chinese herbal medicine are used for quickly detecting；It can be obtained by visual observation
Accurate quantitative detection conclusion out, is not required to be shown with expensive instrument, easy to operate, can grasp without specialized training；It is produced into
This is low, and accuracy is high, reproducible, is extremely suitable for the detection real-time, quickly of Chinese herbal medicine scene and monitoring.
Detailed description of the invention
Fig. 1 is overlooking structure diagram of the invention；
Fig. 2 is side structure schematic view of the invention；
In figure, 1 is lead developing paper, and 2 be cadmium developing paper, and 3 be mercury developing paper, and 4 be arsenic developing paper, and 5 be the residual developing paper of agriculture.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1: Chinese medicine quality is quickly defined the level the production of test paper
Take a height of 8cm × 2cm of length and width × 1cm PVC plastic flitch as substrate, the substrate front side side by side 5 sizes
Identical depth is the cylinder shape groove of 0.5cm, diameter 0.8cm, by lead developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper
It is cut to the circular paper of diameter 0.8cm respectively with the residual developing paper of agriculture and is sequentially placed into substrate recess to get test paper of the present invention.
Embodiment 2: Chinese medicine quality is quickly defined the level the production of test paper
Take a height of 8cm × 2cm of length and width × 1cm PVC plastic flitch as substrate, the substrate front side side by side 5 sizes
Identical depth is the cylinder shape groove of 0.3cm, diameter 1.0cm, by lead developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper
It is cut to the circular paper of diameter 1.0cm respectively with the residual developing paper of agriculture and is sequentially placed into substrate recess to get test paper of the present invention.
Embodiment 3: production developing paper
Lead developing paper: 0.5g rhodizonic acid, 5g PEG-400, the bad hematic acid sodium of 3g are added in 70ml water, and ultrasound is molten
Solution adjusts pH 2.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 60s, in 65 DEG C of drying 30min of temperature；
Cadmium developing paper: by 0.3g potassium hydroxide, 18g PEG-400,0.3g cadion, 3.0mL2.0% phenanthroline, 1g
Sodium thiosulfate, 2g sodium potassium tartrate tetrahydrate are added in 60ml ethyl alcohol, ultrasonic dissolution, adjust pH 7.5 to get solvent 2；By filter paper
Being placed in immersion in solvent 2 is 60s, dries 40min in temperature 70 C；
Mercury developing paper: the Triton X-114 of 1.2g crystal violet, 5g polyvinylpyrrolidone, 5ml are added to 10% second
In alcohol solution, ultrasonic dissolution adjusts pH 8.5 to get solvent 3；Filter paper is placed in solvent 3 and impregnates 60s, in temperature 70 C,
Dry 20min；
Arsenic developing paper: take 3g ammonium molybdate, 0.5g citric acid, 0.1g agarose, 5mL methyl green solution, 2mL sulfuric acid solution,
1mL poly-vinyl alcohol solution adds water to be settled to 20mL, and 70 DEG C of heating 20min are to get solvent 4；Filter paper is placed in solvent 4 and is impregnated
5min is cooled down at room temperature；
The residual developing paper of agriculture: 0.2g thiocarbamide, 0.2g sodium acetate, 0.5g natrium adetate, 0.3g and 50mLpH=7.5 are 1. taken
The acetylcholinesterase of 0.02g is added in phosphate buffer, mixing while stirring, and 0.1g gelatin is added, is allowed to be uniformly dispersed,
The phosphate buffer that pH=7.5 is added is settled to 100mL up to solvent to get solvent 5；Filter paper is placed in solvent 5 and is impregnated
5min is cooled down at room temperature.2. the residual developing solution preparation of agriculture: taking 10ml, 0.2% indophenols acetate solution, be added to 50mL0.1mol/
In the phosphate buffer of LpH=7.5.
Embodiment 4: production developing paper
1) lead developing paper: 0.3g rhodizonic acid, 10g PEG-400, the bad hematic acid sodium of 5g are added in 50ml water, ultrasound
Dissolution adjusts pH 1.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 30s, dries 40min in temperature 70 C；
2) cadmium developing paper: by 0.5g potassium hydroxide, 12g PEG-400,0.1g cadion, 2.0mL2.0% phenanthroline,
2g sodium thiosulfate, 1g sodium potassium tartrate tetrahydrate are added in 50ml ethyl alcohol, ultrasonic dissolution, adjust pH 6.5 to get solvent 2；It will filter
It is 30s that paper, which is placed in immersion in solvent 2, in 65 DEG C of drying 30min of temperature；
3) the Triton X-114 of 0.8 crystal violet, 10g polyvinylpyrrolidone, 3ml mercury developing paper: are added to 5% second
In alcohol solution, ultrasonic dissolution adjusts pH 9.5 to get solvent 3；Filter paper is placed in solvent 3 and impregnates 30s, in 65 DEG C of temperature,
Dry 10min；
4) arsenic developing paper: take 1g ammonium molybdate, 0.2g citric acid, 0.05g agarose, 3mL methyl green solution, 1mL sulfuric acid molten
Liquid, 2mL poly-vinyl alcohol solution add water to be settled to 20mL, and 60 DEG C of heating 15min are to get solvent 4；Filter paper is placed in solvent 4 and is soaked
1min is steeped, is cooled down at room temperature；
5) 0.4g thiocarbamide, 0.3g sodium acetate, 1.0g natrium adetate, 0.2g and 50mLpH=7.5 1. the residual developing paper of agriculture: are taken
Phosphate buffer, the acetylcholinesterase of 0.04g is added while stirring, 0.5g gelatin is added mixing, and it is equal to be allowed to dispersion
It is even, the phosphate buffer of pH=7.5 is added up to solvent and is settled to 100mL to get solvent 5；Filter paper is placed in solvent 5 and is soaked
1min is steeped, is cooled down at room temperature.2. the residual developing solution preparation of agriculture: taking 5ml, 0.2% indophenols acetate solution, be added to
50mL0.1mol/LpH=7.5 phosphate buffer in.
Embodiment 5: colorimetric card production
Lead, cadmium, mercury, arsenic standard color comparison card: the standard solution of lead, cadmium, mercury, arsenic is taken respectively, it is 10ug/ that concentration, which is made, in dilution
The solution of mL, 20ug/mL, 50ug/mL, 100ug/mL, 500ug/mL.Test paper of the present invention is respectively placed in the molten of above-mentioned each concentration
Liquid makes standard color comparison card according to test paper color change.
The residual colorimetric card of agriculture: taking pesticide (carbofuran) in right amount, and 10ug/mL, 20ug/mL, 50ug/mL, 100ug/ is made in dilution
The solution of mL, 500ug/mL.Test paper of the present invention is respectively placed in the solution of above-mentioned each concentration, is made and is marked according to test paper color change
Quasi- colorimetric card.
Embodiment 6: sample detection methods
1. lead, cadmium, mercury, arsenic detect: taking Chinese herbal medicine to crush, 120 DEG C of freeze-day with constant temperature weigh 1.0g, and 20mL concentrated nitric acid is added
With 5mL hydrogen peroxide, it is heated to solution clarification, is let cool to get Chinese herbal medicine solution.Respectively in lead developing paper, cadmium developing paper, mercury colour developing
Paper, arsenic developing paper center at be added dropwise 0.2mL Chinese herbal medicine solution, test paper is horizontally arranged upward, is quantitatively judged after 1min,
The corresponding numerical value of identical with colorimetric card color color range is the detected value of this sample, if the color of test paper is between two color ranges
Take the median of the two.According to measurement result and actual demand is combined, Chinese herbal medicine can be divided into different ranks.
2. the residual detection of agriculture: taking Chinese herbal medicine 2g, 2h is impregnated with the phosphate buffer of 100mLpH7.5, by the solution after immersion
0.2mL drop is horizontally arranged upward in the center of the residual developing paper of agriculture, by test paper, then instills agriculture described in embodiment 3 or embodiment 4
Residual developing solution is quantitatively judged in 0.5-1min, and the corresponding numerical value of identical with colorimetric card color color range is this sample
Detected value, as the color of test paper takes the median of the two between two color ranges.According to measurement result and actual demand is combined,
Chinese herbal medicine can be divided into different ranks.
Comparative example 1: developing paper production
1) lead developing paper: 0.1g rhodizonic acid, 1g PEG-400, the bad hematic acid sodium of 2g are added in 100ml water, ultrasound
Dissolution adjusts pH 1.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 30s, dries 40min in temperature 70 C；
2) cadmium developing paper: by 0.1g potassium hydroxide, 2g PEG-400,0.1g cadion, 1.0mL2.0% phenanthroline, 1g
Sodium thiosulfate, 1g sodium potassium tartrate tetrahydrate are added in 100ml ethyl alcohol, ultrasonic dissolution, adjust pH 6.5 to get solvent 2；By filter paper
Being placed in immersion in solvent 2 is 30s, in 65 DEG C of drying 30min of temperature；
3) the Triton X-114 of 0.5g crystal violet, 2g polyvinylpyrrolidone, 1ml mercury developing paper: are added to 5% second
In alcohol solution, ultrasonic dissolution adjusts pH 8.5 to get solvent 3；Filter paper is placed in solvent 3 and impregnates 60s, in 65 DEG C of temperature,
Dry 10min；
4) arsenic developing paper: 1g ammonium molybdate, 1mL methyl green solution, 1mL sulfuric acid solution, 1mL poly-vinyl alcohol solution are taken, water is added
20mL is settled to get solvent 4；Filter paper is placed in solvent 4 and impregnates 1min, is cooled down at room temperature；
5) the residual developing paper of agriculture: 1. taking the phosphate buffer of 0.1g thiocarbamide, 0.1g sodium acetate and 50mLpH=7.5, mixing,
The acetylcholinesterase of 0.01g is added while stirring, 0.1g gelatin is added, is allowed to be uniformly dispersed, the phosphate of pH=7.5 is added
Buffer is settled to 100mL up to solvent to get solvent 5；Filter paper is placed in solvent 5 and impregnates 5min, is cooled down at room temperature；2. agriculture
Residual developing solution preparation: 10ml, 0.2% indophenols acetate solution are taken, the phosphate buffer of 50mL0.1mol/LpH=7.5 is added to
In.
Comparative example 2: developing paper production
1) lead developing paper: 1g rhodizonic acid, 20g PEG-400, the bad hematic acid sodium of 10g are added in 50ml water, ultrasound
Dissolution adjusts pH1.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 30s, dries 40min in temperature 70 C；
2) cadmium developing paper: by 2g potassium hydroxide, 20g PEG-400,5g cadion, 2.0mL2.0% phenanthroline, 10g sulphur
Sodium thiosulfate, 5g sodium potassium tartrate tetrahydrate are added in 50ml ethyl alcohol, ultrasonic dissolution, adjust pH6.5 to get solvent 2；Filter paper is placed in
Impregnating in solvent 2 is 30s, in 65 DEG C of drying 30min of temperature；
3) mercury developing paper: the Triton X-114 of 1.5g crystal violet, 12g polyvinylpyrrolidone, 10ml are added to
In 10% ethanol water, ultrasonic dissolution adjusts pH 8.5 to get solvent 3；Filter paper is placed in solvent 3 and impregnates 60s, Yu Wen
70 DEG C of degree, drying 20min；
4) arsenic developing paper: take 10g ammonium molybdate, 1g citric acid, 1g agarose, 8mL methyl green solution, 5mL sulfuric acid solution,
5mL poly-vinyl alcohol solution adds water to be settled to 20mL, and 70 DEG C of heating 20min are to get solvent 4；Filter paper is placed in solvent 4 and is impregnated
5min is cooled down at room temperature；
5) the residual developing paper of agriculture: 1. taking the phosphate buffer of 1g thiocarbamide, 1g sodium acetate and 50mLpH=7.5, mixing, while stirring
The acetylcholinesterase that 0.1g is added in side is mixed, 1g gelatin is added, is allowed to be uniformly dispersed, the phosphate buffer of pH=7.5 is added
100mL is settled to up to solvent to get solvent 5；Filter paper is placed in solvent 5 and impregnates 5min, is cooled down at room temperature；2. the residual colour developing of agriculture
Liquid preparation: 10ml, 0.2% indophenols acetate solution are taken, is added in the phosphate buffer of 50mL0.1mol/LpH=7.5.
Comparative example 3: developing paper production
1) lead developing paper: pass through the immersion of reaction solution 1, soaking time 20s, in temperature 50 C, vacuum degree 100- in advance
30min is dried in the environment of Pa；The solvent of reaction solution 1 is 120ml ethyl alcohol, and solute is 0.5g sodium hydroxide, 5 polyvinylpyrrolidines
Ketone, 0.3g cadion, 30ml 10% triton x-100, ultrasonic dissolution, adjust pH 6.5；
2) cadmium developing paper: pass through the immersion of reaction solution 2, soaking time 20s, in temperature 60 C, vacuum degree 100Pa in advance
In the environment of dry 30min；The solvent of reaction solution 2 is 80ml ultrapure water, and solute is 0.3g copper reagent, 5g polyvinylpyrrolidine
5% triton x-100 of ketone, 20-30ml, ultrasonic dissolution adjust pH 6.5；
3) mercury developing paper: a. passes through the immersion of reaction solution 3, soaking time 20s, in temperature 60 C, vacuum degree in advance
30min is dried in the environment of 100Pa.B. the solvent of reaction solution 3 be 120ml hot ethanol, solute be 1.2g diphenylcarbazide,
10% triton x-100 of 10g polyvinylpyrrolidone, 30ml, ultrasonic dissolution adjust pH 8.5；
4) arsenic developing paper: 10g ammonium molybdate, 10mL methyl green solution, 5mL sulfuric acid solution, 5mL poly-vinyl alcohol solution are taken, is added
Water is settled to 10mL to get solvent 4；Filter paper is placed in solvent 4 and impregnates 1min, is cooled down at room temperature；
5) the residual developing paper of agriculture: 1. according to the ratio of 2g:100mL, the phosphoric acid of agarose and 0.1mol/L, pH=7.0 is prepared
The mixed liquor of salt buffer is added in pressure cooker and is heated to 0.05KPa, keeps 20min, and normal pressure is cooled to 37 DEG C naturally, then drips
Add 20ml, 13%NaOH solution, 1.5ml dimethyl sulfate phenol is added dropwise, 37 DEG C of constant temperature carry out methylation reaction, add dropwise while stirring
Enter the CH3COOH of 0.5mol/L, adjusts the acetylcholinesterase of pH to neutral pH=7.0,0.02g, what addition had been disinfected
37 DEG C of paraffin oils, are allowed to be uniformly dispersed, and are put into 0 DEG C of ice-water bath and solidify, and 5000r/min is centrifuged off oily phase 3min, and repeatedly 3
It is secondary, go oil removing mutually to get temperature sensitive microcapsules；2. prepared by developing solution: taking 0.5ml, 0.2% indophenols acetic acid esters mother liquor, be added to
In the phosphate buffer of 50mL, 0.1mol/L, pH=7.0；3. prepared by test paper: by temperature sensitive microcapsules and developing solution in 1:1 ratio
It after mixing, is coated on former test paper, and air-dries obtain temperature sensitive microencapsulation coating at room temperature, coating layer thickness is about 100-200 μm.
Experimental example: detection limit measurement
Chinese herbal medicine lead solution: the concentration in Example 5 is the lead standard solution of 500ug/mL and the medium-height grass that lead is not detected
Drug solns in 1:1 ratio mix to get.
Chinese herbal medicine cadmium solution: the concentration in Example 5 is the cadmium standard solution of 500ug/mL and the medium-height grass that lead is not detected
Drug solns in 1:1 ratio mix to get.
Chinese herbal medicine mercury solution: the concentration in Example 5 is the mercury standard solution of 500ug/mL and the medium-height grass that lead is not detected
Drug solns in 1:1 ratio mix to get.
Chinese herbal medicine arsenic solution: the concentration in Example 5 is the lead standard solution of 500ug/mL and the medium-height grass that arsenic is not detected
Drug solns in 1:1 ratio mix to get.
Pesticide made of Chinese medicinal herbs solution: the concentration in Example 5 is the pesticide solution of 500ug/mL and pesticide residue is not detected
Chinese herbal medicine solution in 1:1 ratio mix to get.
Experimental method: taking above-mentioned solution 0.2mL, with the embodiment of the present invention 3, embodiment 4 and 1~comparative example of comparative example 3
Developing paper detection, if detection, solution is diluted, until it can not detect.It the results are shown in Table 3.
Table 3: detection limit (ppm)
| Sample solution | Embodiment 3 | Embodiment 4 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Chinese herbal medicine lead solution | 2.5 | 2.5 | 50 | 25 | 25 |
| Chinese herbal medicine cadmium solution | 5 | 5 | 25 | 50 | 25 |
| Chinese herbal medicine mercury solution | 2.5 | 2.5 | 25 | 50 | 50 |
| Chinese herbal medicine arsenic solution | 5 | 5 | 100 | 100 | 50 |
| Pesticide made of Chinese medicinal herbs solution | 2.5 | 2.5 | 100 | 100 | 50 |
The experimental results showed that using test paper of the present invention, the detection of lead is limited to 2.5ppm, the detection of cadmium is limited to 5ppm, mercury
Detection is limited to 2.5ppm, the detection of arsenic is limited to 5ppm, the detection of pesticide is limited to 2.5ppm, and the test paper of comparative example 1-3 is to above-mentioned object
The detection limit of matter is all larger than 25ppm, and the detection limit of test paper of the present invention is better than comparative example.
Test paper of the present invention is only made of substrate and developing paper two parts, structurally and operationally all very simple, this test paper can be
Lead, cadmium, mercury, 4 kinds of elements of arsenic and pesticide residue content in Chinese herbal medicine are used for quickly detecting simultaneously in 0.5-1min, detection limit
Within 5ppm, detection effect is significant, can be realized the quick detection of Chinese herbal medicine, can be to medium-height grass according to this test paper testing result
Medicine carries out grade classification.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (8)
- The test paper 1. Chinese medicine quality is quickly defined the level, which is characterized in that be made of 1 piece of substrate and 5 pieces of developing papers, the colour developing paper bag Lead developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper, the residual developing paper of agriculture are included, the developing paper includes following processing step:1) 0.3-0.5g rhodizonic acid, 5-10g PEG-400, the bad hematic acid sodium of 3-5g lead developing paper: are added to 50-70ml water In, ultrasonic dissolution adjusts pH 1.5-2.5 to get solvent 1；Filter paper is placed in solvent 1 and impregnates 30-60s, in temperature 65-70 DEG C drying 30-40min；2) cadmium developing paper: by 0.3-0.5g potassium hydroxide, 12-18g PEG-400,0.1-0.3g cadion, 2.0- 3.0mL2.0% phenanthroline, 1-2g sodium thiosulfate, 1-2g sodium potassium tartrate tetrahydrate are added in 50-60ml ethyl alcohol, ultrasonic dissolution, PH 6.5-7.5 is adjusted to get solvent 2；Filter paper is placed in solvent 2 and is impregnated for 30-60s, in 65-70 DEG C of drying 30- of temperature 40min；3) mercury developing paper: the Triton X-114 of 0.8-1.2g crystal violet, 5-10g polyvinylpyrrolidone, 3-5ml are added to In 5%-10% ethanol water, ultrasonic dissolution adjusts pH 8.5-9.5 to get solvent 3；Filter paper is placed in solvent 3 and is impregnated 30-60s, in 65-70 DEG C of temperature, drying 10-20min；4) 1-3g ammonium molybdate, 0.2-0.5g citric acid, 0.05-0.1g agarose, 3-5mL methyl green solution, 1- arsenic developing paper: are taken 2mL sulfuric acid solution, 1-2mL poly-vinyl alcohol solution add water to be settled to 20mL, and 60-70 DEG C of heating 15-20min is to get solvent 4；It will Filter paper, which is placed in solvent 4, impregnates 1-5min, cools down at room temperature；5) 0.2-0.4g thiocarbamide, 0.2-0.3g sodium acetate, 0.5-1.0g natrium adetate, 0.2-0.3g bis- the residual developing paper of agriculture: are taken The phosphate buffer of sodium diethyldithiocarbamate and 50mLpH=7.5, mixing are added 0.02-0.04g's while stirring Acetylcholinesterase is added 0.1g-0.5g gelatin, is allowed to be uniformly dispersed, the phosphate buffer of pH=7.5 is added up to solvent 100mL is settled to get solvent 5；Filter paper is placed in solvent 5 and impregnates 1-5min, is cooled down at room temperature.
- The test paper 2. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the substrate front side side by side The identical depth of 5 sizes is the cylinder shape groove of 0.3-0.5cm, diameter 0.8-1.0cm, and the groove is for placing the lead Developing paper, cadmium developing paper, mercury developing paper, arsenic developing paper and the residual developing paper of agriculture.
- The test paper 3. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 1) are as follows: will The bad hematic acid sodium of 0.5g rhodizonic acid, 5g PEG-400,3g is added in 70ml water, ultrasonic dissolution, adjusts pH 2.5 to get molten Agent 1；Filter paper is placed in solvent 1 and impregnates 60s, in 65 DEG C of drying 30min of temperature.
- The test paper 4. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 2) are as follows: will 0.3g potassium hydroxide, 18g PEG-400,0.3g cadion, 3.0mL2.0% phenanthroline, 1g sodium thiosulfate, 2g tartaric acid Potassium sodium is added in 60ml ethyl alcohol, ultrasonic dissolution, adjusts pH7.5 to get solvent 2；Filter paper is placed in solvent 2 and is impregnated for 60s, 40min is dried in temperature 70 C.
- The test paper 5. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 3) are as follows: will 1.2g crystal violet, 5g polyvinylpyrrolidone, 5ml Triton X-114 be added in 10% ethanol water, ultrasonic dissolution, PH 8.5 is adjusted to get solvent 3；Filter paper is placed in solvent 3 and impregnates 60s, in temperature 70 C, drying 20min.
- The test paper 6. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 4) are as follows: take 3g Ammonium molybdate, 0.5g citric acid, 0.1g agarose, 5mL methyl green solution, 2mL sulfuric acid solution, 1mL poly-vinyl alcohol solution add water fixed Hold to 20mL, 70 DEG C of heating 20min are to get solvent 4；Filter paper is placed in solvent 4 and impregnates 5min, is cooled down at room temperature.
- The test paper 7. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 5) are as follows: take 0.2g thiocarbamide, 0.2g sodium acetate, 0.5g natrium adetate, 0.3g sodium diethyldithiocarbamate and 50mLpH=7.5 The acetoacetyl cholinesterase of 0.02g is added in phosphate buffer, mixing while stirring, and 0.1g gelatin is added, and it is equal to be allowed to dispersion It is even, the phosphate buffer of pH=7.5 is added up to solvent and is settled to 100mL to get solvent 5；Filter paper is placed in solvent 5 and is soaked 5min is steeped, is cooled down at room temperature.
- The test paper 8. Chinese medicine quality according to claim 1 is quickly defined the level, which is characterized in that the step 5) further relates to show The preparation of color liquid, preparation step are as follows: take 5-10ml, 0.2% indophenols acetate solution, be added to 50mL0.1mol/LpH=7.5's In phosphate buffer.
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