CN107184735A - A kind of Camellia nitidissima antiallergy spray and preparation method thereof - Google Patents

Abstract

The invention relates to the field of traditional Chinese medicine, in particular to a golden camellia anti-allergy spray and a preparation method thereof. The spray is extracted from the following traditional Chinese medicine compositions: golden camellia peel, Bupleurum chinensis, comfrey, ebony plum, schisandra chinensis, licorice, asarum and cassia twig. The present invention also provides a preparation method of the spray, wherein the above-mentioned raw materials are extracted, filtered, and dried to obtain active ingredients, and then a certain dose of odorant and preservative is added, and filtered again to make Camellia antiallergic spray . Through the rational compatibility of various traditional Chinese medicines, the present invention effectively exerts the medicinal effect of Camellia japonica fruit peel, etc., and extracts the active ingredients of the traditional Chinese medicine composition, so that the prepared Camellia camellia anti-allergy spray is not only convenient to use, but also has a high dosage. Small in size, it is conducive to the absorption of active ingredients and can effectively treat allergic diseases.

Description

A kind of camellia antiallergic spray and preparation method thereof

technical field

The invention relates to the field of traditional Chinese medicine, in particular to a golden camellia anti-allergy spray and a preparation method thereof.

Background technique

In recent years, allergic diseases have shown a sharp upward trend with an annual increase rate of 20%, such as allergic asthma, allergic rhinitis, early spring hay fever, contact dermatitis, eczema, hay fever, and drug allergy. It has become a very noticeable social problem, and the highest rising rate is allergic asthma and allergic rhinitis. It not only brings a lot of inconvenience to daily life, causes mental pain, and reduces the quality of life, but also lasts for a long time, some become intractable diseases, and some lead to death.

Camellia Camellia, which is the same as tea, Nanshan tea, camellia, and oil tea, is a plant of the genus Camellia in the family Theaceae. Camellia chrysalis is famous for its yellow petals. The botanical name is Camellia chrysantha(Hu)Tuyama. Shrubs to small trees. Clinical studies have shown that golden camellia has the functions of inhibiting tumors, enhancing immunity, anti-aging, balancing various physiological functions of the human body, lowering blood pressure, enhancing blood vessel elasticity and myocardial contractility, enhancing liver metabolism, and activating various enzymes in the human body. . Wang Yongqi and others confirmed that Camellia japonica peel has anti-allergic properties through experiments (Wang Yongqi, Peng Xiao, Tang Qian et al. Anti-IgE-mediated type I allergic reaction activity screening of Camellia japonica group plants, [J], Zhongnan Pharmacy, 7(10): 721-724.). At present, there are few literatures about camellia japonica, especially its plant peel, used in the treatment of anti-allergic diseases, and the anti-allergic research on the peel of camellia camellia is not deep enough.

Bupleurum is commonly used to relieve symptoms. Also known as Dixun, Shancai, Mushroom Grass, and Firewood, it is bitter in nature and slightly cold, and returns to the Liver and Gallbladder Meridians. It has the effects of reconciling the exterior and interior, soothing the liver and promoting yang.

Comfrey, cold in nature, sweet and salty in the mouth, GUIXIN and Liver Meridian. Cure mainly trusted subordinate evil qi, five jaundices, invigorate the middle and replenish qi, sharpen the nine orifices, and unblock the waterways.

Dark plum, sour in taste, astringent, flat in nature. Return liver, spleen, lung, large intestine channel. It has the effects of astringing the lungs, astringing the intestines, promoting body fluid, and soothing roundworms.

Schisandra chinensis has the effects of astringing the lungs, nourishing the kidneys, promoting body fluid, sweating, and astringent essence.

Licorice, slightly gas, sweet and special. Functions and indications: heat-clearing and toxic substances removing, eliminating phlegm and relieving cough, abdominal distension and so on.

Herba Asarum has the effects of dispelling wind and cold, clearing the orifices and relieving pain, warming the lung and transforming drink.

Cinnamon Twig, pungent, sweet, warm in nature. Guixin, lung, bladder channel. It has the effects of sweating and relieving muscles, warming the meridians, helping yang to transform qi, dispelling cold and relieving pain.

At present, the products using camellia camellia as raw materials are mostly beverages, which cannot meet the needs of people and waste the medicinal resources of camellia camellia. Moreover, the traditional Chinese medicine has a bitter smell and poor taste, so many people are unwilling to take it.

Contents of the invention

The purpose of the present invention is to overcome the defects of the above problems, and provide a golden camellia antiallergy spray for treating diseases such as allergic asthma and allergic rhinitis.

The purpose of the present invention can be achieved through the following technical solutions:

A golden camellia anti-allergy spray, comprising the following raw materials in parts by weight: 15-20 parts of golden camellia peel, 15-20 parts of bupleurum, 10-20 parts of comfrey, 10-15 parts of ebony plum, 10-15 parts of schisandra, 5-8 parts of licorice, 10-15 parts of asarum, 10-15 parts of cassia twig.

The preparation method of above-mentioned golden camellia antiallergic spray comprises the following steps:

(1) Leaching

Wash Bupleurum, Comfrey, Black Plum, Schisandra, Licorice, Asarum, and Guizhi according to the proportion of raw materials, and grind them to obtain raw material powder. Soak the raw material powder with 80% ethanol with 5 times the mass for 12 hours, and then add 10 times the mass 80% ethanol percolation for 4 hours, recovering ethanol, and obtaining the composition extract;

Washing and pulverizing the Camellia japonica peel, putting it into a percolator, continuously adding 90% ethanol 10 times its mass for percolation from the upper part, collecting the leachate, and obtaining the Camellia japonica peel extract;

(2) Filtration and drying

Mix the composition extract obtained in step (1) and the Camellia japonica peel extract evenly, add 2% NaOH solution 4 times the mass of the raw material, stir well, precipitate, filter, add a little excess hydrochloric acid to the filtrate and stir well, and control the pH value of the filtrate 3 to 4, then filter, wash the precipitate with water until neutral, and dry it under vacuum at 60°C to obtain a medicinal powder;

(3) preparation

The medicinal powder obtained in the step (2) is dissolved by adding 2 times the mass of 80% ethanol and 5 times the mass of propylene glycol as a composite solvent, then adding a solubilizer, an odor correcting agent and a preservative, stirring and mixing, and then filtering to obtain Camellia antibacterial Allergy spray.

In the preparation method of the above-mentioned camellia antiallergic spray, the solubilizer added in step (3) is Tween 80, and the added amount is 5% of the mass of the composite solvent used in step (3).

In the preparation method of the above-mentioned golden camellia antiallergic spray, the odor correcting agent added in the step (3) is borneol, and the added amount is 5% of the mass of the medicinal powder obtained in the step (2).

In the preparation method of the above-mentioned golden camellia anti-allergy spray, the preservative added in the step (3) is ethyl p-hydroxybenzoate, and the added amount is 3% of the mass of the medicinal powder obtained in the step (2).

Compared with the prior art, the present invention has the following beneficial effects:

1. Through the reasonable compatibility of various traditional Chinese medicines, the present invention effectively exerts the medicinal effect of Camellia japonica peel, etc., and effectively treats diseases such as allergic rhinitis and allergic asthma;

2. By extracting the active ingredients of the traditional Chinese medicine composition, the prepared golden camellia anti-allergy spray is not only convenient to use, but also has a small amount of use, which is beneficial to the absorption of the active ingredients and effectively treats allergic diseases.

detailed description

The present invention will be further described below in conjunction with specific examples.

Example 1:

A golden camellia anti-allergy spray, comprising the following raw materials in parts by weight: 18 parts of golden camellia peel, 15 parts of Bupleurum, 10 parts of comfrey, 10 parts of black plum, 10 parts of Schisandra chinensis, 6 parts of licorice, 10 parts of asarum, cinnamon 10 branches.

The preparation method of above-mentioned golden camellia antiallergic spray comprises the following steps:

(1) Leaching

Wash 15g of Bupleurum bupleuri, 10g of comfrey, 10g of black plum, 10g of schisandra, 6g of licorice, 10g of asarum, and 10g of cassia twigs, and grind them to obtain raw material powder. The raw material powder is soaked in 5 times the amount of 80% ethanol for 12 hours, and then Add 10 times the amount of 80% ethanol for percolation for 4 hours, recover the ethanol, and obtain the composition extract;

Wash and pulverize 18g of camellia pericarp, put it into a percolator, continuously add 10 times the amount of 90% ethanol from the upper part to percolate, collect the leachate, and obtain the extract of camellia camellia pericarp;

(2) Filtration and drying

Mix the composition extract obtained in step (1) and Camellia japonica peel extract evenly, add 2% NaOH solution of 4 times the amount of the drug, stir well, precipitate, filter, add a little excess hydrochloric acid to the filtrate and stir well to make the pH value 3 , and then filtered, the precipitate was washed with water until neutral, and vacuum-dried at 60°C to obtain a medicinal powder;

(3) Preparation

With the medicated powder obtained in step (2), add 2 times the amount of 80% ethanol and 5 times the amount of propylene glycol to dissolve as a composite solvent, then add 30ml Tween 80, 5g borneol and 3g ethyl p-hydroxybenzoate, stir and mix, and then After filtering, golden camellia antiallergic spray can be obtained.

Example 2:

A golden camellia anti-allergy spray, comprising the following raw materials in parts by weight: 15 parts of golden camellia peel, 15 parts of Bupleurum, 10 parts of comfrey, 15 parts of black plum, 15 parts of Schisandra chinensis, 6 parts of licorice, 10 parts of asarum, cinnamon 10 branches.

The preparation method of above-mentioned golden camellia antiallergic spray comprises the following steps:

(1) Leaching

Wash 15g of Bupleurum radix, 10g of comfrey, 15g of black plum, 15g of Schisandra chinensis, 6g of licorice, 10g of asarum, and 10g of cassia twig, and grind them to obtain raw material powder. The raw material powder is soaked in 5 times the amount of 80% ethanol for 12 hours, and then Add 10 times the amount of 80% ethanol for percolation for 4 hours, recover the ethanol, and obtain the composition extract;

Wash and pulverize 15g of camellia pericarp, put it into a percolator, add 10 times the amount of 90% ethanol continuously from the top to percolate, collect the leachate, and obtain the extract of camellia camellia pericarp;

(2) Filtration and drying

Mix the composition extract obtained in step (1) and Camellia japonica peel extract evenly, add 2% NaOH solution of 4 times the amount of the drug, stir well, precipitate, filter, and add a little excess hydrochloric acid to the filtrate and stir well to make the pH value 4 , and then filtered, the precipitate was washed with water until neutral, and vacuum-dried at 60°C to obtain a medicinal powder;

(3) preparation

With the medicated powder obtained in step (2), add 2 times the amount of 80% ethanol and 5 times the amount of propylene glycol as a composite solvent to dissolve, then add 35ml Tween 80, 5g Borneolum and 3g ethyl p-hydroxybenzoate, stir and mix, and then After filtering, golden camellia antiallergic spray can be obtained.

Example 3:

A golden camellia anti-allergy spray, comprising the following raw materials in parts by weight: 20 parts of golden camellia peel, 15 parts of Bupleurum, 10 parts of comfrey, 10 parts of black plum, 15 parts of schisandra, 8 parts of licorice, 10 parts of asarum, cinnamon Branch 15 copies.

The preparation method of above-mentioned golden camellia antiallergic spray comprises the following steps:

(1) Leaching

Wash 15g of bupleurum, 10g of comfrey, 10g of black plum, 15g of schisandra, 8g of licorice, 10g of asarum, and 15g of cassia twig, and grind them to obtain raw material powder. The raw material powder is soaked in 5 times the amount of 80% ethanol for 12 hours, and then Add 10 times the amount of 80% ethanol for percolation for 4 hours, recover the ethanol, and obtain the composition extract;

Wash and pulverize 20 g of camellia pericarp, put it into a percolator, continuously add 10 times the amount of 90% ethanol from the upper part to percolate, collect the leachate, and obtain the extract of camellia camellia pericarp;

(2) Filtration and drying

Mix the composition extract obtained in step (1) and Camellia japonica peel extract evenly, add 2% NaOH solution of 4 times the amount of the drug, stir well, precipitate, filter, and add a little excess hydrochloric acid to the filtrate and stir well to make the pH value 4 , filter, wash the precipitate with water until neutral, and dry it under vacuum at 60°C to obtain a medicinal powder;

(3) preparation

With the medicated powder obtained in step (2), add 2 times the amount of 80% ethanol and 5 times the amount of propylene glycol as a composite solvent to dissolve, then add 35ml Tween 80, 5g Borneolum and 3g ethyl p-hydroxybenzoate, stir and mix, and then After filtering, golden camellia antiallergic spray can be obtained.

Animal toxicity test:

1. Take 90 experimental mice, half male and half female, weighing 140-180g, and divide them into three groups 1-3, 30 mice in each group. The mice in each group have good signs and no abnormalities in body weight and other physiological performances, which are comparable .

2. The three groups of mice were given the anti-allergic sprays of camellia japonica prepared in Examples 1 to 3 of the present invention, respectively, and administered by intragastric administration three times a day for one month in a row. Observe and record the signs of all mice during the experiment Condition.

3. During the experiment, the signs of all the mice showed no abnormalities, and no mice died after the experiment. 5 mice were randomly selected from each group for anatomical examination, and no abnormalities were found in the main organs. From the experimental results, The golden camellia antiallergic spray of the invention has no obvious toxicity and can be used safely.

For the conditioning of allergic disease states to improve clinical observation statistics:

1. Case selection: 90 clinically diagnosed patients with allergic diseases, aged 20-60 years, 60 males and 30 females, were divided into three experimental groups 1-3, 30 cases in each group.

2. Experimental method: The sprays prepared in Examples 1 to 3 of the present invention were given to experimental groups 1 to 3 respectively, three times a day for one month continuously, and the recovery of the patients was observed and recorded.

3. Efficacy criteria: effective: the clinical symptoms of allergic diseases disappear, all signs return to normal, and there is no recurrence within half a year; markedly effective: the clinical symptoms of allergic diseases are improved, and the signs of patients are significantly better than before taking the medicine; invalid: the condition No improvement or aggravation.

4. Experimental data statistics:

category Number of cases efficient markedly effective invalid Adverse reactions Efficient Example 1 30 10 20 0 0 100.0 Example 2 30 12 18 0 0 100.0 Example 3 30 10 17 3 0 90.0

5. Experimental results: As can be seen from the above experimental data, the total effective rate of the Camellia antiallergic spray prepared by the present invention for the treatment of allergic diseases is 96.7%, and 90 patients have no adverse reactions. Therefore, it is explained that, The golden camellia antiallergic spray of the invention is a safe and effective drug for treating allergic diseases, and has good clinical popularization significance.

Claims (5)

1. a kind of Camellia nitidissima antiallergy spray, it is characterised in that include the raw material of following parts by weight：Golden flower tea peels 15~20 Part, 15~20 parts of radix bupleuri, 10~20 parts of Asian puccoon, 10~15 parts of dark plum, 10~15 parts of the fruit of Chinese magnoliavine, 5~8 parts of radix glycyrrhizae, asarum 10~ 15 parts, 10~15 parts of cassia twig.

2. the preparation method of Camellia nitidissima antiallergy spray as claimed in claim 1, it is characterised in that comprise the following steps：

(1) extract

Radix bupleuri, Asian puccoon, dark plum, the fruit of Chinese magnoliavine, radix glycyrrhizae, asarum, cassia twig are cleaned by raw material proportioning, crushes, raw material powder is obtained, by original Feed powder 80% ethanol of 5 times of quality soaks 12h, then adds 80% ethanol percolation 4h of 10 times of quality, reclaims ethanol, is combined Thing extract solution；

Golden flower tea peels are cleaned and crushed, percolator is put into, 90% ethanol percolation of its 10 times of quality is continuously added to from top, are received Collect leachate, obtain Camellia nitidissima Fruit peel extract；

(2) filter, dry

The composition extract solution and Camellia nitidissima Fruit peel extract that are obtained in step (1) is well mixed, add 4 times of quality of raw material 2%NaOH solution is sufficiently stirred for, and is precipitated, and filtering, filtrate adds slightly excessive hydrochloric acid to be sufficiently stirred for, control filtrate pH value 3~4, after Filter, precipitation is washed at neutrality, 60 DEG C and is dried in vacuo, and obtains medicinal powder；

(3) prepare

The medicinal powder that will be obtained in step (2), adds 2 times of ethanol of quality 80% and 5 times of quality propane diols dissolve as double solvents, Solubilizer, flavoring agent and preservative are added, is filtered after stirring and evenly mixing, produces Camellia nitidissima antiallergy spray.

3. the preparation method of Camellia nitidissima antiallergy spray as claimed in claim 2, it is characterised in that added in step (3) Plus solubilizer is Tween 80, the amount of addition is 5% of double solvents quality used in step (3).

4. the preparation method of Camellia nitidissima antiallergy spray as claimed in claim 2, it is characterised in that added in step (3) Plus flavoring agent is borneol, the amount of addition is 5% of gained medicinal powder quality in step (2).

5. the preparation method of Camellia nitidissima antiallergy spray as claimed in claim 2, it is characterised in that added in step (3) Adding preservative agent is ethyl-para-hydroxybenzoate, and the amount of addition is 3% of gained medicinal powder quality in step (2).