CN107176691A - It is a kind of for spacetabs type microorganism improver of water quality of aquaculture and preparation method thereof - Google Patents
It is a kind of for spacetabs type microorganism improver of water quality of aquaculture and preparation method thereof Download PDFInfo
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- CN107176691A CN107176691A CN201710399002.6A CN201710399002A CN107176691A CN 107176691 A CN107176691 A CN 107176691A CN 201710399002 A CN201710399002 A CN 201710399002A CN 107176691 A CN107176691 A CN 107176691A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention belongs to technical field of aquaculture, and in particular to a kind of for spacetabs type microorganism improver of water quality of aquaculture and preparation method thereof.Preparing the raw material of the improver of water quality includes following material：30%~50% complex micro organism fungicide, 40%~60% nutritional agents, 1%~5% thickener, 1%~10% stabilizer, wherein microorganism live bacteria quantity is more than 2,000,000,000/g.The present invention utilizes the growth metabolism of functional microorganism, the harmful substance that fish growth breeding is influenceed in water body is utilized, converted and degraded, so as to play a part of water body purification, the spacetabs type improver of water quality improves the shortcoming of traditional biological modifying agent simultaneously, avoid the fluctuation of water body index, the abnormal physiology of aquatic livestock will not be caused to react, and reduce water temperature, water quality and the influence of the extraneous factor to action effect such as medicine is residual, action period is long, water body environment can be kept for a long time, reduce aquaculture cost.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of spacetabs type microorganism water quality for aquaculture
Modifying agent and preparation method thereof.
Background technology
China is world fisheries big country, is also world aquaculture big country, and culture fishery is quickly grown in recent years, intensive
Changing high-density breeding turns into development trend, but also causes breeding ecological environment to be destroyed.Due to not focusing on planning, cultivation side
Formula is unreasonable, blindly pursues high density and high yield, feed and medicine is excessively used, and seriously exceeds water body bearing capacity, fish disease
It is constantly serious with quality problem.The accumulation of the remaining of medicine and feed, biological excreta, dead volume and Organic residues, causes water
Body severe contamination.The quality of water quality directly influences the health of aquatic animal, as environmental health cultivates going deep into for idea, cultivates
Water quality management and the use of improver of water quality turn into one of emphasis of daily cultivation.
The material of influence aquaculture is mainly some compositions such as ammonia nitrogen, nitrite, sulfide, suspension, makes at present
Improver of water quality mainly have physical improver of water quality, chemical type improver of water quality and bion improver of water quality, it is necessary to
Selected accordingly according to cultivation species and cultivation stages.Physical improver of water quality mainly will be harmful by suction-operated
Material is adsorbed, and effect is quick, can microelement-supplementing, but being used for a long time can cause substrate to deteriorate, and without degraded
Ability, actual effect is poor.Chemical type improver of water quality is mainly oxidant, ion-exchanger, flocculant etc., makees with antibacterial
With, it can be reacted with harmful substance so that it is degraded, but using effect is influenceed than larger, and to aquatic products by condition of water quality
There is animal excitant, particularly seedling stage animal to have larger adverse effect.Bion improver of water quality is current using most
Kind, the growth and breeding of pathogenic bacteria can be suppressed, the harmful substance in water body is thoroughly decomposed, it is and not unfavorable to aquatic livestock
Influence, repairs the ecological environment of water body.But the use of microorganism formulation is by temperature, medicine be residual etc. that external condition is influenceed larger,
Use requirement is higher, and actual effect has larger fluctuation, and the growth of microorganism needs oxygen, can be led using rear amount reproduction
The decline of dissolved oxygen in water body is caused, while the metabolite produced can change the pH environment of water body, aquatic livestock is caused necessarily
The influence of degree.
The content of the invention
In consideration of it, the present invention provides a kind of spacetabs type microorganism improver of water quality for aquaculture, it is micro- using function
Biological growth metabolism, is utilized, converted and is degraded on the harmful substance that fish growth breeding is influenceed in water body, so as to play
The effect of water body purification, while the spacetabs type improver of water quality improves the shortcoming of traditional biological modifying agent, it is to avoid water body refers to
Target fluctuation, will not cause the abnormal physiology of aquatic livestock to react, and reduce water temperature, water quality and medicine it is residual etc. it is extraneous because
Influence of the element to action effect, the action period is long, and water body environment can be kept for a long time, reduces aquaculture cost.
The technical solution adopted by the present invention is as follows：
A kind of spacetabs type microorganism improver of water quality for aquaculture, prepare the improver of water quality raw material include with
Lower material：30%~50% complex micro organism fungicide, 40%~60% nutritional agents, 1%~5% thickener, 1%~10% stabilization
Agent, wherein microorganism live bacteria quantity is more than 2,000,000,000/g.
The complex micro organism fungicide includes Rhodopseudomonas palustris, bacillus subtilis, enterococcus faecalis, Candida utilis ferment
Female, nitrobacteria and nitrite bacteria.
The nutritional agents is the one or more in glucose, molasses, fine bran, xylo-oligosaccharide, maltodextrin.
The thickener is the one or more in xanthans, alginic acid, heteroglycan.
The stabilizer is the one or more in bentonite, precipitated calcium carbonate, diatomite.
A kind of preparation method of the spacetabs type microorganism improver of water quality for aquaculture as described above, including with
Lower step：
(1) complex micro organism fungicide, wherein Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrite bacteria are prepared
Prepared using liquid-state fermentation technology, bacillus subtilis and candida utili are prepared using solid-state fermentation process；
(2) after bacteria fermentation terminates, strain is premixed, Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, Asia
Nitrobacteria premix is prepared into liquid mix bacterium agent, and bacillus subtilis and candida utili premix are prepared into solid Mixed Microbes
Agent, is then prepared into complex micro organism fungicide by liquid mix bacterium agent and solid mix bacterium agent premix；
(3) nutritional agents, thickener, stabilizer are individually premixed；
(4) complex micro organism fungicide after premix, nutritional agents, thickener, stabilizer are mixed, various material additions
Preferred sequence be nutritional agents, thickener, stabilizer, complex micro organism fungicide；
(5) mixed material is dried by low temp dryer；
(6) by the mixed material briquet after drying, and cut, cutting block size is according to feeding aquatic livestock
Type is determined.
In the step (1), the culture medium composition of Rhodopseudomonas palustris is sodium acetate 0.2%~0.6%, ammonium chloride
0.1%~0.2%, sodium acid carbonate 0.1%~0.2%, ammonium dihydrogen phosphate 0.05%~0.1%, magnesium sulfate 0.05%~
0.1%th, sodium chloride 0.5%~2.0%, yeast extract 0.5%~1.0%, remaining be after distilled water, constant volume regulation pH value to 6.5
~7.0,121 DEG C of 20~30min of autoclave sterilization；The condition of culture of Rhodopseudomonas palustris is 60 watts of incandescent lamp irradiations, temperature
28 DEG C~35 DEG C of degree, 3~5d of quiescent culture.
In the step (1), the culture medium of nitrobacteria and nitrite bacteria composition for natrium nitrosum 0.05%~
0.15%th, disodium hydrogen phosphate 0.05%~0.1%, sodium dihydrogen phosphate 0.01%~0.05%, sodium acid carbonate 0.1%~0.2%,
Magnesium sulfate 0.02%~0.5%, manganese sulfate 0.003%~0.006%, remaining be after distilled water, constant volume regulation pH value to 7.0~
7.5,121 DEG C of 20~30min of autoclave sterilization；The condition of culture of nitrobacteria and nitrite bacteria is temperature 35 DEG C~40
DEG C, air agitation culture 3~5 days.
In the step (1), the culture medium composition of enterococcus faecalis is peptone 3%~5%, dusty yeast 1%~3%, Portugal
Grape sugar 2%~5%, ammonium citrate 0.1%~0.3%, magnesium sulfate 0.01%~0.03%, manganese sulfate 0.005%~0.01%,
Calcium carbonate 1.0%~1.5%, Tween 80 1mL/L, remaining be after distilled water, constant volume regulation pH value to 6.5~7.2,115 DEG C high
Warm 20~30min of autoclaving；The condition of culture of enterococcus faecalis is 35 DEG C~40 DEG C of temperature, quiescent culture 3~4 days.
In the step (1), the material-water ratio of the culture medium of bacillus subtilis is 1:1~1.5, wherein solid material, which is constituted, is
Wheat bran 60%~80%, rice husk 10%~30%, corn flour 5%~10%, dregs of beans 5%~10%, magnesium sulfate 0.01%~
0.05%, 121 DEG C of 30~50min of autoclave sterilization；The condition of culture of bacillus subtilis is 35 DEG C~40 DEG C of temperature, interval
Stirring, is cultivated 2~3 days.
In the step (1), the material-water ratio 1 of the culture medium of candida utili:1~1.5, wherein solid material composition is bran
Skin 70%~90%, glucose 2%~10%, urea 2%~10%, biotin 0.01%~0.05%, 121 DEG C of HTHPs
Sterilize 30~50min；The condition of culture of candida utili is 35 DEG C~40 DEG C of temperature, and interval stirring is cultivated 2~3 days.
In the step (2), the percentage by weight of each microbial inoculum is in complex micro organism fungicide：Rhodopseudomonas palustris 5%
~10%, enterococcus faecalis 10%~30%, nitrobacteria 20%~30%, nitrite bacteria 15%~25%, bacillus subtilis
Bacterium 10%~20%, candida utili 20%~30%.
In the step (5), drying temperature is 30 DEG C~50 DEG C, and mixed material moisture is dried to 15%~20%.
In the step (6), during cutting, hardness is controlled 2.0~3.0.
Compared with prior art, the present invention has following excellent technique effect：
(1) product of the present invention is evenly distributed, consumption reduction.Modifying agent can be suspended in water body, overcome modifying agent and sunk
Enter the water-bed and shortcoming of the sphere of action of restriction micro-organisms, can be moved with water body, make modifying agent uniform point up and down in water body
Cloth, so as to reduce modifying agent consumption.
(2) product long action time of the present invention, catharsis can be played for a long time.Microbial bacterial agent is utilized in modifying agent
Nutritional ingredient Reproduction simultaneously produces beneficial metabolic product, and constantly viable bacteria and metabolite are discharged into water body, not only ensured
The activity of microorganism, and the aquatic environment of water body can be maintained for a long time.
(3) product action temperature of the present invention and, microorganism slowly discharges.After quick-acting microorganism modifying agent use in water body
The more metabolite of accumulation, can cause the larger fluctuation of the indexs such as Dissolved Oxygen in Water, pH value in amount reproduction, and short time,
So that aquatic livestock produces abnormal physiology reaction；Product microorganism of the present invention slowly discharges, it is ensured that microorganism is in purifying water body
During aquatic livestock is not influenceed.
(4) product of the present invention is wide using scope, and the environmental condition such as residual to temperature, medicine requires low.The problems such as antibiotic residue
The action effect of microorganism improver of water quality can be caused to decline, and spacetabs type modifying agent is long due to deenergized period, can make function
Microorganism is to the continuous adaptation of environment and domestication, so as to preferably play catharsis.
Embodiment
The present invention is further explained with reference to embodiments：
Embodiment 1
A kind of spacetabs type microorganism improver of water quality for aquaculture, prepare the improver of water quality raw material include with
Lower material：50% complex micro organism fungicide, 35% nutritional agents, 5% thickener, 10% stabilizer.
The complex micro organism fungicide includes Rhodopseudomonas palustris, bacillus subtilis, enterococcus faecalis, Candida utilis ferment
Female, nitrobacteria and nitrite bacteria.
The nutritional agents is glucose, molasses, fine bran and xylo-oligosaccharide.
The thickener is alginic acid and heteroglycan.
The stabilizer is bentonite and precipitated calcium carbonate.
A kind of preparation method of spacetabs type microorganism improver of water quality for aquaculture, comprises the following steps：
(1) complex micro organism fungicide, wherein Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrite bacteria are prepared
Prepared using liquid-state fermentation technology, bacillus subtilis and candida utili are prepared using solid-state fermentation process；
The culture medium composition of Rhodopseudomonas palustris is sodium acetate 0.2%, ammonium chloride 0.1%, sodium acid carbonate 0.2%, phosphorus
Acid dihydride ammonium 0.05%, magnesium sulfate 0.06%, sodium chloride 0.5%, yeast extract 1.0%, remaining is to adjust pH after distilled water, constant volume
It is worth to 7.0,121 DEG C of autoclave sterilization 30min；Condition of culture is 60 watts of incandescent lamp irradiations, 32 DEG C of temperature, quiescent culture 4d；
The culture medium composition of nitrobacteria and nitrite bacteria is natrium nitrosum 0.15%, disodium hydrogen phosphate 0.05%, phosphorus
Acid dihydride sodium 0.05%, sodium acid carbonate 0.1%, magnesium sulfate 0.02%, manganese sulfate 0.005%, remaining is tune after distilled water, constant volume
PH value is saved to 7.5,121 DEG C of autoclave sterilization 30min；Condition of culture is 37 DEG C of temperature, air agitation culture 4 days；
The culture medium composition of enterococcus faecalis is peptone 3%, dusty yeast 1%, glucose 2%, ammonium citrate 0.1%, sulphur
Sour magnesium 0.03%, manganese sulfate 0.005%, calcium carbonate 1.5%, Tween 80 1mL/L, remaining is to adjust pH value after distilled water, constant volume
To 6.5,115 DEG C of autoclave sterilization 30min；Condition of culture is 35 DEG C of temperature, quiescent culture 3 days；
The material-water ratio of the culture medium of bacillus subtilis is 1:1.2, wherein solid material composition is wheat bran 59.95%, rice husk
20%th, corn flour 10%, dregs of beans 10%, magnesium sulfate 0.05%, 121 DEG C of autoclave sterilization 50min；Condition of culture is temperature 37
DEG C, interval stirring is cultivated 3 days；
The material-water ratio 1 of the culture medium of candida utili:1, wherein solid material composition is wheat bran 89.95%, glucose
5%, urea 5%, biotin 0.05%, 121 DEG C of autoclave sterilization 40min；Condition of culture be 35 DEG C of temperature, interval stirring,
Culture 2 days.
It is respectively that Rhodopseudomonas palustris reaches using the microbial inoculum viable count of above-mentioned culture medium and condition of culture fermenting and producing
4.2×109Cfu/g, enterococcus faecalis reaches 8.7 × 108Cfu/g, nitrobacteria reaches 3.2 × 108Cfu/g, nitrite bacteria reaches
To 1.8 × 108Cfu/g, bacillus subtilis reaches 3.4 × 1010Cfu/g, candida utili reaches 8.6 × 108cfu/g。
(2) after bacteria fermentation terminates, strain is premixed, Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, Asia
Nitrobacteria premix is prepared into liquid mix bacterium agent, and bacillus subtilis and candida utili premix are prepared into solid Mixed Microbes
Agent, is then prepared into complex micro organism fungicide by liquid mix bacterium agent and solid mix bacterium agent premix；
The percentage by weight of each microbial inoculum is in complex micro organism fungicide：Rhodopseudomonas palustris 5%, enterococcus faecalis 30%,
Nitrobacteria 20%, nitrite bacteria 15%, bacillus subtilis 10%, candida utili 20%.
(3) nutritional agents, thickener, stabilizer are individually premixed.
(4) each material after premix is carried out according to the order of nutritional agents, thickener, stabilizer, complex micro organism fungicide
Premix.
(5) by mixed material by low temp dryer, drying drying temperature is 30 DEG C, mixed material moisture dry to
20%.
(6) by the mixed material briquet after drying, and cut, hardness is controlled in 2.0, cutting cube diameter
30mm, thickness 20mm.Viable bacteria content is 2,700,000,000/g in finished product.
Prepare the fry phase special improver of water quality of aquatic livestock using this method, and by the product release to length 3~
The pond of 4cm young age mandarin fish carries out application test, result of the test such as table 1：Unit (mg/L).
1 fry phase of table aquatic livestock application test
Compared with control group, water pH value and dissolved oxygen (DO) fluctuating range very little after test group is delivered, during using one section
Between after pH stable young age aquatic livestock during certain level, use there is not the reaction of any abnormal physiology；Although
Spacetabs type improver of water quality, but because the degree of water pollution of young age aquatic livestock is relatively light, COD after dispensing
(COD), ammonia nitrogen (NH4 +- N), nitroso nitrogen (NO2- N) reduction of speed is very fast, the range of decrease of the 5th day is respectively 52.35%, 69.77%,
64.52%, the 10th day decrease by 85.52%, 94.19% and 96.77%.Product is up to one month deenergized period, release
Secondary dispensing is not needed in phase, water body indices stabilization in latter two moon is delivered, water quality keeps good, significantly saves and cultivates into
This.Microorganism in modifying agent produces beneficial metabolic product in growth course, can suppress the pathogenic bacteria breeding in water body, examination
The survival rate of fish fry that group is tested than control group improves 34%.
Embodiment 2
A kind of spacetabs type microorganism improver of water quality for aquaculture, prepare the improver of water quality raw material include with
Lower material：40% complex micro organism fungicide, 45% nutritional agents, 5% thickener, 10% stabilizer.
The complex micro organism fungicide includes Rhodopseudomonas palustris, bacillus subtilis, enterococcus faecalis, Candida utilis ferment
Female, nitrobacteria and nitrite bacteria.
The nutritional agents is molasses, fine bran, xylo-oligosaccharide and maltodextrin.
The thickener is alginic acid and heteroglycan.
The stabilizer is bentonite and diatomite.
A kind of preparation method of spacetabs type microorganism improver of water quality for aquaculture, comprises the following steps：
(1) complex micro organism fungicide, wherein Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrite bacteria are prepared
Prepared using liquid-state fermentation technology, bacillus subtilis and candida utili are prepared using solid-state fermentation process；
Rhodopseudomonas palustris culture medium composition for sodium acetate 0.3%, ammonium chloride 0.14%, sodium acid carbonate 0.17%,
Ammonium dihydrogen phosphate 0.06%, magnesium sulfate 0.08%, sodium chloride 0.8%, yeast extract 1.0%, remaining is regulation after distilled water, constant volume
PH value is to 7.0,121 DEG C of autoclave sterilization 30min；Condition of culture is 60 watts of incandescent lamp irradiations, 35 DEG C of temperature, quiescent culture
4d；
The culture medium composition of nitrobacteria and nitrite bacteria is natrium nitrosum 0.13%, disodium hydrogen phosphate 0.06%, phosphorus
Acid dihydride sodium 0.04%, sodium acid carbonate 0.12%, magnesium sulfate 0.05%, manganese sulfate 0.004%, after remaining is distilled water, constant volume
PH value is adjusted to 7.5,121 DEG C of autoclave sterilization 30min；Condition of culture is 35 DEG C of temperature, air agitation culture 4 days；
The culture medium composition of enterococcus faecalis is peptone 3.5%, dusty yeast 1.2%, glucose 2.2%, ammonium citrate
0.14%th, magnesium sulfate 0.03%, manganese sulfate 0.006%, calcium carbonate 1.2%, Tween 80 1mL/L, remaining is distilled water, constant volume
PH value is adjusted afterwards to 6.8,115 DEG C of autoclave sterilization 30min；Condition of culture is 35 DEG C of temperature, quiescent culture 3 days；
The material-water ratio of the culture medium of bacillus subtilis is 1:1.2, wherein solid material composition is wheat bran 64.96%, rice husk
20%th, corn flour 5%, dregs of beans 10%, magnesium sulfate 0.04%, 121 DEG C of autoclave sterilization 50min；Condition of culture is temperature 35
DEG C, interval stirring is cultivated 3 days；
The material-water ratio 1 of the culture medium of candida utili:1.1, wherein solid material composition is wheat bran 84.95%, glucose
10%, urea 5%, biotin 0.05%, 121 DEG C of autoclave sterilization 40min；Condition of culture be 40 DEG C of temperature, interval stirring,
Culture 2 days.
It is respectively that Rhodopseudomonas palustris reaches using the microbial inoculum viable count of above-mentioned culture medium and condition of culture fermenting and producing
3.7×109Cfu/g, enterococcus faecalis reaches 9.2 × 108Cfu/g, nitrobacteria reaches 3.1 × 108Cfu/g, nitrite bacteria reaches
To 2.0 × 108Cfu/g, bacillus subtilis reaches 4.4 × 1010Cfu/g, candida utili reaches 7.5 × 108cfu/g。
(2) after bacteria fermentation terminates, strain is premixed, Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, Asia
Nitrobacteria premix is prepared into liquid mix bacterium agent, and bacillus subtilis and candida utili premix are prepared into solid Mixed Microbes
Agent, is then prepared into complex micro organism fungicide by liquid mix bacterium agent and solid mix bacterium agent premix；
The percentage by weight of each microbial inoculum is in complex micro organism fungicide：Rhodopseudomonas palustris 10%, enterococcus faecalis 10%,
Nitrobacteria 20%, nitrite bacteria 15%, bacillus subtilis 20%, candida utili 25%.
(3) nutritional agents, thickener, stabilizer are individually premixed.
(4) each material after premix is carried out according to the order of nutritional agents, thickener, stabilizer, complex micro organism fungicide
Premix.
(5) by mixed material by low temp dryer, drying drying temperature is 50 DEG C, mixed material moisture dry to
20%.
(6) by the mixed material briquet after drying, and cut, hardness is controlled in 2.5, cutting cube diameter
30mm, thickness 50mm.Viable bacteria content is 4,500,000,000/g in finished product.
Prepare the adult fish phase special improver of water quality of aquatic livestock using this method, and by the product release to length 10~
The pond of 15cm carp carries out application test, result of the test such as table 2：Unit (mg/L).
2 adult fish phase of table aquatic livestock application test
The product release to pond is subjected to application test, compared with control group, COD after test group is delivered
(COD), ammonia nitrogen (NH4 +- N), nitroso nitrogen (NO2- N) reduction of speed is slow, the range of decrease of the 5th day is respectively 43.87%, 49.26%,
57.47%, the 10th day decrease by 74.66%, 91.18% and 86.21%.Product is up to two months deenergized period, release
Secondary dispensing is not needed in phase, water body indices are stable in three months after dispensing, water quality keeps good, significantly save and cultivate into
This.Microorganism in modifying agent produces beneficial metabolic product in growth course, can suppress the pathogenic bacteria breeding in water body, examination
Test group reduces by 59% than the incidence of disease of control group, output increased 19%, and economic benefit is obvious.
Embodiment 3
A kind of spacetabs type microorganism improver of water quality for aquaculture, prepare the improver of water quality raw material include with
Lower material：40% complex micro organism fungicide, 45% nutritional agents, 5% thickener, 10% stabilizer.
The complex micro organism fungicide includes Rhodopseudomonas palustris, bacillus subtilis, enterococcus faecalis, Candida utilis ferment
Female, nitrobacteria and nitrite bacteria.
The nutritional agents is molasses, fine bran and xylo-oligosaccharide.
The thickener is alginic acid and heteroglycan.
The stabilizer is bentonite and diatomite.
A kind of preparation method of spacetabs type microorganism improver of water quality for aquaculture, comprises the following steps：
(1) complex micro organism fungicide, wherein Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrite bacteria are prepared
Prepared using liquid-state fermentation technology, bacillus subtilis and candida utili are prepared using solid-state fermentation process；
Rhodopseudomonas palustris culture medium composition for sodium acetate 0.4%, ammonium chloride 0.15%, sodium acid carbonate 0.15%,
Ammonium dihydrogen phosphate 0.08%, magnesium sulfate 0.06%, sodium chloride 0.9%, yeast extract 1.0%, remaining is regulation after distilled water, constant volume
PH value is to 7.0,121 DEG C of autoclave sterilization 30min；Condition of culture is 60 watts of incandescent lamp irradiations, 35 DEG C of temperature, quiescent culture 4
My god；
The culture medium composition of nitrobacteria and nitrite bacteria is natrium nitrosum 0.1%, disodium hydrogen phosphate 0.08%, phosphoric acid
Sodium dihydrogen 0.05%, sodium acid carbonate 0.13%, magnesium sulfate 0.05%, manganese sulfate 0.005%, remaining is tune after distilled water, constant volume
PH value is saved to 7.5,121 DEG C of autoclave sterilization 30min；Condition of culture is 37 DEG C of temperature, air agitation culture 5 days；
The culture medium composition of enterococcus faecalis is peptone 3.5%, dusty yeast 1.4%, glucose 2%, ammonium citrate
0.15%th, magnesium sulfate 0.02%, manganese sulfate 0.008%, calcium carbonate 1.3%, Tween 80 1mL/L, remaining is distilled water, constant volume
PH value is adjusted afterwards to 6.5,115 DEG C of autoclave sterilization 30min；Condition of culture is 37 DEG C of temperature, quiescent culture 3 days；
The material-water ratio of the culture medium of bacillus subtilis is 1:1.4, wherein solid material composition is wheat bran 59.95%, rice husk
30%th, corn flour 5%, dregs of beans 5%, magnesium sulfate 0.05%, 121 DEG C of autoclave sterilization 50min；Condition of culture is temperature 37
DEG C, interval stirring is cultivated 3 days；
The material-water ratio 1 of the culture medium of candida utili:1, wherein solid material composition is wheat bran 79.96%, glucose
10%, urea 10%, biotin 0.04%, 121 DEG C of autoclave sterilization 40min；Condition of culture is 35 DEG C of temperature, is intermittently turned over
Material, is cultivated 2 days.
It is respectively that Rhodopseudomonas palustris reaches using the microbial inoculum viable count of above-mentioned culture medium and condition of culture fermenting and producing
4.1×109Cfu/g, enterococcus faecalis reaches 8.2 × 108Cfu/g, nitrobacteria reaches 3.7 × 108Cfu/g, nitrite bacteria reaches
To 2.6 × 108Cfu/g, bacillus subtilis reaches 4.0 × 1010Cfu/g, candida utili reaches 8.5 × 108cfu/g。
(2) after bacteria fermentation terminates, strain is premixed, Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, Asia
Nitrobacteria premix is prepared into liquid mix bacterium agent, and bacillus subtilis and candida utili premix are prepared into solid Mixed Microbes
Agent, is then prepared into complex micro organism fungicide by liquid mix bacterium agent and solid mix bacterium agent premix；
The percentage by weight of each microbial inoculum is in complex micro organism fungicide：Rhodopseudomonas palustris 10%, enterococcus faecalis 10%,
Nitrobacteria 25%, nitrite bacteria 20%, bacillus subtilis 15%, candida utili 20%.
(3) nutritional agents, thickener, stabilizer are individually premixed.
(4) each material after premix is carried out according to the order of nutritional agents, thickener, stabilizer, complex micro organism fungicide
Premix.
(5) by mixed material by low temp dryer, drying drying temperature is 40 DEG C, mixed material moisture dry to
20%.
(6) by the mixed material briquet after drying, and cut, hardness is controlled in 3.0, cutting cube diameter
50mm, thickness 30mm.Viable bacteria content is 3,200,000,000/g in finished product.
The special improver of water quality of shrimps aquatic livestock is prepared using this method, and by the product release into the prawn culturing pool
Carry out application test, result of the test such as table 3：Unit (mg/L).
The shrimps aquatic livestock application test of table 3
The product release to pond is subjected to application test, compared with control group, COD after test group is delivered
(COD), ammonia nitrogen (NH4 +- N), nitroso nitrogen (NO2- N) reduction of speed is slow, the range of decrease of the 5th day is respectively 47.24%, 42.24%,
51.32%, the 10th day decrease by 79.88%, 89.66% and 86.84%.Product is up to two months deenergized period, release
Secondary dispensing is not needed in phase, water body indices are stable in three months after dispensing, water quality keeps good, significantly save and cultivate into
This.Microorganism in modifying agent produces beneficial metabolic product in growth course, can suppress the pathogenic bacteria breeding in water body, examination
Test group reduces by 42% than the incidence of disease of control group, output increased 24%, and economic benefit is obvious.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (19)
1. a kind of spacetabs type microorganism improver of water quality for aquaculture, it is characterised in that prepare the improver of water quality
Raw material includes following material：30%~50% complex micro organism fungicide, 40%~60% nutritional agents, 1%~5% thickener, 1%
~10% stabilizer, wherein microorganism live bacteria quantity is more than 2,000,000,000/g.
2. the spacetabs type microorganism improver of water quality according to claim 1 for aquaculture, it is characterised in that described
Complex micro organism fungicide includes Rhodopseudomonas palustris, bacillus subtilis, enterococcus faecalis, candida utili, nitrobacteria
And nitrite bacteria.
3. the spacetabs type microorganism improver of water quality according to claim 1 for aquaculture, it is characterised in that described
Nutritional agents is the one or more in glucose, molasses, fine bran, xylo-oligosaccharide, maltodextrin.
4. the spacetabs type microorganism improver of water quality according to claim 1 for aquaculture, it is characterised in that described
Thickener is the one or more in xanthans, alginic acid, heteroglycan.
5. the spacetabs type microorganism improver of water quality according to claim 1 for aquaculture, it is characterised in that described
Stabilizer is the one or more in bentonite, precipitated calcium carbonate, diatomite.
6. a kind of preparation method of spacetabs type microorganism improver of water quality for aquaculture as described in claim 1-5,
It is characterised in that it includes following steps：
(1) complex micro organism fungicide is prepared, wherein Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrite bacteria is used
Prepared by liquid-state fermentation technology, bacillus subtilis and candida utili are prepared using solid-state fermentation process；
(2) after bacteria fermentation terminates, strain is premixed, Rhodopseudomonas palustris, enterococcus faecalis, nitrobacteria, nitrosation
Bacterium premix is prepared into liquid mix bacterium agent, and bacillus subtilis and candida utili premix are prepared into solid mix bacterium agent,
Then liquid mix bacterium agent and solid mix bacterium agent premix are prepared into complex micro organism fungicide；
(3) nutritional agents, thickener, stabilizer are individually premixed；
(4) complex micro organism fungicide after premix, nutritional agents, thickener, stabilizer are mixed, it is excellent that various materials are added
Choosing order is nutritional agents, thickener, stabilizer, complex micro organism fungicide；
(5) mixed material is dried by low temp dryer；
(6) by the mixed material briquet after drying, and cut, cutting block size is according to the type for feeding aquatic livestock
It is determined that.
7. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (1), the culture medium composition of Rhodopseudomonas palustris is sodium acetate 0.2%~0.6%, ammonium chloride
0.1%~0.2%, sodium acid carbonate 0.1%~0.2%, ammonium dihydrogen phosphate 0.05%~0.1%, magnesium sulfate 0.05%~
0.1%th, sodium chloride 0.5%~2.0%, yeast extract 0.5%~1.0%, remaining be after distilled water, constant volume regulation pH value to 6.5
~7.0,121 DEG C of 20~30min of autoclave sterilization.
8. the preparation method of the spacetabs type microorganism improver of water quality according to claim 7 for aquaculture, it is special
Levy and be, in the step (1), the condition of culture of Rhodopseudomonas palustris irradiates for 60 watts of incandescent lamps, 28 DEG C~35 DEG C of temperature,
3~5d of quiescent culture.
9. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (1), the culture medium of nitrobacteria and nitrite bacteria composition for natrium nitrosum 0.05%~
0.15%th, disodium hydrogen phosphate 0.05%~0.1%, sodium dihydrogen phosphate 0.01%~0.05%, sodium acid carbonate 0.1%~0.2%,
Magnesium sulfate 0.02%~0.5%, manganese sulfate 0.003%~0.006%, remaining be after distilled water, constant volume regulation pH value to 7.0~
7.5,121 DEG C of 20~30min of autoclave sterilization.
10. the preparation method of the spacetabs type microorganism improver of water quality according to claim 9 for aquaculture, it is special
Levy and be, in the step (1), the condition of culture of nitrobacteria and nitrite bacteria is 35 DEG C of temperature~40 DEG C, air agitation
Culture 3~5 days.
11. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (1), the culture medium composition of enterococcus faecalis is peptone 3%~5%, dusty yeast 1%~3%, grape
Sugar 2%~5%, ammonium citrate 0.1%~0.3%, magnesium sulfate 0.01%~0.03%, manganese sulfate 0.005%~0.01%, carbon
Sour calcium 1.0%~1.5%, Tween 80 1mL/L, remaining be after distilled water, constant volume regulation pH value to 6.5~7.2,115 DEG C of high temperature
20~30min of autoclaving.
12. the preparation method of the spacetabs type microorganism improver of water quality according to claim 11 for aquaculture, its
It is characterised by, in the step (1), the condition of culture of enterococcus faecalis is 35 DEG C~40 DEG C of temperature, quiescent culture 3~4 days.
13. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (1), the material-water ratio of the culture medium of bacillus subtilis is 1:1~1.5, wherein solid material, which is constituted, is
Wheat bran 60%~80%, rice husk 10%~30%, corn flour 5%~10%, dregs of beans 5%~10%, magnesium sulfate 0.01%~
0.05%, 121 DEG C of 30~50min of autoclave sterilization.
14. the preparation method of the spacetabs type microorganism improver of water quality according to claim 13 for aquaculture, its
It is characterised by, in the step (1), the condition of culture of bacillus subtilis is 35 DEG C~40 DEG C of temperature, interval stirring, culture 2
~3 days.
15. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (1), the material-water ratio 1 of the culture medium of candida utili:1~1.5, wherein solid material composition is bran
Skin 70%~90%, glucose 2%~10%, urea 2%~10%, biotin 0.01%~0.05%, 121 DEG C of HTHPs
Sterilize 30~50min.
16. the preparation method of the spacetabs type microorganism improver of water quality according to claim 15 for aquaculture, its
It is characterised by, in the step (1), the condition of culture of candida utili is 35 DEG C~40 DEG C of temperature, interval stirring, culture 2
~3 days.
17. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (2), the percentage by weight of each microbial inoculum is in complex micro organism fungicide：Rhodopseudomonas palustris 5%~
10%th, enterococcus faecalis 10%~30%, nitrobacteria 20%~30%, nitrite bacteria 15%~25%, bacillus subtilis
10%~20%, candida utili 20%~30%.
18. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (5), drying temperature is 30 DEG C~50 DEG C, and mixed material moisture is dried to 15%~20%.
19. the preparation method of the spacetabs type microorganism improver of water quality according to claim 6 for aquaculture, it is special
Levy and be, in the step (6), during cutting, hardness is controlled 2.0~3.0.
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