CN107167621B - Multi-channel detection method for magnetic particle chemiluminescence immunoassay analyzer - Google Patents
Multi-channel detection method for magnetic particle chemiluminescence immunoassay analyzer Download PDFInfo
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- CN107167621B CN107167621B CN201610127319.XA CN201610127319A CN107167621B CN 107167621 B CN107167621 B CN 107167621B CN 201610127319 A CN201610127319 A CN 201610127319A CN 107167621 B CN107167621 B CN 107167621B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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Abstract
The invention discloses a multi-channel parallel method of a magnetic particle chemiluminescence immunoassay analyzer, which comprises the steps of detachably loading a reaction cup into a cup strip, and filling a detection sample into a sample loading channel; the cup strip is conveyed to a reagent adding channel, a detection reagent is added into the reaction cup and conveyed to a reagent mixing channel; conveying to an incubation system to incubate the detection sample in the reaction cup; conveying to a washing channel for washing; conveying the cup strips to a substrate filling and uniformly mixing channel for filling the substrates; conveying to a detection waiting channel for detection; grabbing the reaction cups in the detection waiting channel one by one to a detection system for detection; and transporting the empty cup strip to a sample adding channel; wherein, the number of the sample adding channel, the reagent adding channel and the washing channel is at least two. The invention ensures the detection flexibility, greatly improves the detection efficiency, can recycle the cup strips, and has less material consumption and lower detection cost.
Description
Technical field
The present invention relates to a kind of magnetic microparticle chemiluminescence immune assay instrument multicenter detecting methods, belong to detection and analysis technology
Field.
Background technique
Magnetic particle luminescence immunoassay is a kind of detection that chemiluminescence immunoassay method is combined with magnetic particle isolation technics
Method can be in magnetic particle surface or magnetic since magnetic particle has the characteristics that magnetic responsiveness, at low cost, less energy consumption and pollution-free
The functional group (such as amino, hydroxyl, sulfydryl and ethylene oxide) of microparticle surfaces is by bioactivity such as enzyme, antibody, oligonucleotides
Substance is fixed, and mostly using ELISA Plate as solid phase carrier, suspension magnetic particle has higher traditional immune detection as carrier
Specific area, can more adequately and example reaction, the flexible Application of externally-applied magnetic field, has more compared with ELISA Plate carrier in addition
The advantages that high sensitivity, faster detection speed and preferably repeatability, therefore magnetic particle luminescence immunoassay instrument is immune
Analysis field has its original advantage.
Magnetic particle luminescence immunoassay instrument when being detected, is needed to be loaded using reaction cup and transports analysis sample, and
It also needs to undergo filling reagent, incubation, washing plus substrate and blending process, traditional magnetic particle electrochemiluminescent immunoassay point before testing
Analyzer generallys use disc type single channel circulation conveying structure, and multiple reaction cups are usually fixed as one group of integrally conveying or single
Reaction cup conveys one by one, and when being conveyed using integral way, flexibility is poor, it is difficult to adapt to the detection of small lot diversification sample presentation
It needs, when one cup conveys one by one, transfer efficiency is lower, seriously limits the detection efficiency of magnetic particle luminescence immunoassay instrument.
In addition, number of the magnetic particle luminescence immunoassay according to filling reagent, analytic process are also divided into one-step method and two
Footwork, when using two-step method, after first time filling reagent is incubated for, it is also necessary to reagent be added again and then carry out subsequent analysis again
Process, when part, detection sample needs to be detected with one-step method, when partially needing to be detected with two-step method, traditional magnetic particle electrochemiluminescent immunoassay
Analyzer can only carry out one by one, it is difficult to meet it and detect needs.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of magnetic microparticle chemiluminescence immune assay instrument multi-channel detection side
Method to improve the detection efficiency of magnetic microparticle chemiluminescence immune assay instrument, and guarantees its flexibility.
To achieve the above object, technical solution of the present invention is as follows:
A kind of magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method, comprising the following steps:
Reaction cup is sorted and is detachably loaded with respect in glass item, is put into plus sample channel is to fill detection sample；
Cup item after filling to be detected to sample is transported to reagent adding channel, and is added by reagent loading system into reaction cup
Infuse detection reagent；
By the cup item after filling detection reagent, passes through reagent and mix channel progress reagent mixing；
Cup item after mixing is transported to incubation system to be incubated for the detection sample in reaction cup；
Sample is detected after being incubated for, corresponding cup item is transported to washing channel, reaction cup is washed；
After reaction cup washing, corresponding cup item is transported to substrate filling and mixes channel filling substrate and mixes；
Corresponding cup item after filling substrate and mixing, which is transported to detection, waits channel etc. to be detected；
It will test and the reaction cup on channel inner cup item is waited to grab detected into detection system one by one by handgrip；
And it will test and the empty cup item for having removed reaction cup in channel is waited to be transported to plus sample channel；
Wherein, described plus sample channel, reagent adding channel and washing channel are at least two.
By adopting the above technical scheme, multiple reaction cups one is formed to transport, when detection system detects using handgrip by
One crawl, that is, ensure that flexibility, while substantially increasing detection efficiency, and due to using multi-channel parallel mode, part is detected
Sample using two-step method detect when do not influence other one-step method detection efficiency, in addition, cup item can circulating repetition utilize, reduce
Consumptive material dosage, has saved testing cost.
To guarantee to transport efficiency, the transport of the cup item send glass trolley to carry out by least two, respectively send the dynamic of glass trolley
Make to control by control system, control system can monitor the working condition for sending glass trolley, and when needing to transport cup, control is current
What is be in idle condition send a glass trolley to be transported.
Preferably, reaction cup upper end two sides are equipped with crawl card slot, to facilitate handgrip to grab, cup item is strip knot
The reaction cup card slot of at least one up and down, the shape and reaction cup outer rim of the reaction cup card slot is distributed in structure along its length
Shape is adapted.
For convenience of positioning and transporting the cup both ends equipped with positioning region, which protrudes reaction cup card
Slot end face, the positioning region are equipped with transfer hole, and positioning region two sides are equipped with the locating groove of arc, described plus sample channel, additional examination
Agent channel and washing channel are groove-like structure corresponding with cup strip, and when cup item is located on each channel, it is fixed to can use
Position card slot is positioned, and when transport, corresponding construction on glass trolley is sent to protrude into transfer hole to facilitate dragging to be transported.
To further increase detection efficiency, the reagent loading system includes at least one reagent filling needle, can be simultaneously
Multiple reaction cups on cup item fill reagent.
The utility model has the advantages that
Multiple mutually independent reaction cups are placed on same cup item and transport by the present invention, final to detect preceding each step
It is all made of multi-channel parallel mode, detection system is grabbed one by one using handgrip and is detected, while guaranteeing to detect flexibility,
Substantially increase detection efficiency, and cup item can circulating repetition utilize, consumptive material dosage is few, and testing cost is lower.
Detailed description of the invention
Fig. 1 is flow chart of the invention；
Fig. 2 is the structure principle chart of magnetic microparticle chemiluminescence immune assay instrument in the present invention；
Fig. 3 is the structural schematic diagram of reaction cup；
Fig. 4 is the structural schematic diagram of cup item；
Fig. 5 is the structural schematic diagram that reaction cup is packed into after cup item；
Fig. 6 is structural schematic diagram when cup item is located at each channel.
Specific embodiment
The invention will be further described with attached drawing with reference to embodiments.
As shown in Figures 1 to 6, a kind of magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method, including following step
It is rapid:
S1: after multiple reaction cups 1 are sorted by row's cup system, being detachably loaded with respect in glass item 2 by sending cup to move cup handgrip,
It is put into and adds in sample channel A through the filling needle or TP filling detection samples in filling sample system；
S2: the cup item 2 after filling to be detected to sample send a glass trolley 4a to be transported to reagent adding channel B by first, and passes through
Reagent loading system fills detection reagent into reaction cup 1；
S3: it send a glass trolley 4a to be transported to reagent by first the cup item 2 after filling detection reagent and mixes channel C, mix
Detection sample and reagent in reaction cup 1；
S4: it send glass trolley 4b to be transported to incubation system D by second after mixing and the detection sample in reaction cup 1 is incubated
It educates；
S41: in the case of the special reagent of part, after detecting sample incubation, corresponding cup item 2 is transported to reagent adding channel B, weight
Multiple S2~S4 step；
S5: detection sample is after being incubated for, and second send glass trolley 4b that corresponding cup item 2 is transported to washing channel E, to reaction cup
1 is washed, the impurity after being incubated for cleaning in reaction cup 1；
S51: when using two-step method, after reaction cup 1 is washed, glass trolley 4a is sent to be transported to corresponding cup item 2 by first
Reagent adding channel B repeats S2~S5 step；
S6: after reaction cup 1 is washed, send glass trolley 4a or second that glass trolley 4b is sent to be transported to corresponding cup item 2 by first
Substrate filling and mixing channel F filling substrate simultaneously mix；
S7: after filling substrate, glass trolley 4a or second is sent to send glass trolley 4b that corresponding cup item 2 is transported to inspection by first
It surveys and waits channel G etc. to be detected；
S8: it will test and the reaction cup 1 on channel G inner cup item 2 is waited to be grabbed one by one by detection shifting cup handgrip 3 to detection system
It is detected in system H, the reaction cup 1 after the completion of detecting is moved cup handgrip 3 using the detection and lost in trash repository；
S9: it finally will test and the empty cup item 2 for having removed reaction cup 1 in the G of channel waited to send a glass trolley 4a by first
It is transported to and adds sample channel A, wait next testing process.
The transport of cup item 2 send glass trolley 4 to complete by least two, send the structure of glass trolley 4 in Chinese invention patent
It is able to disclosure in the documents such as application CN104646343A, CN104535760A, therefore not to repeat here, in the present embodiment, send cup small
Vehicle 4 is preferably two, including first send glass trolley 4a and second to send glass trolley 4b, arranged in parallel, above-mentioned steps S2, S3,
S51, S9 send a glass trolley 4a individually to complete by first, and step S4, S5 individually send glass trolley 4b to complete by second, step S6 and
S7 then by moving trolley 4 control system detection two send be in idle condition in glass trolley 4 wherein one or two simultaneously into
Row, step S41 first send a glass trolley 4b to be transported to reagent and mixes channel C by second, then send a glass trolley 4a to be transported to additional examination by first
Agent channel B.
Fig. 3 and Fig. 4 illustrates the specific structure of reaction cup 1 and cup item 2 respectively, it can be seen that reaction cup 1 is substantially presented
The cup-shape of end opening lower end closed, the upper end end circumferentially outwardly protrude.
The whole elongated structure of cup item 2, both ends are equipped with positioning region 22, middle part be distributed with along its length multiple shapes with
The corresponding reaction cup card slot 21 of 1 outer rim shape of reaction cup, reaction cup card slot 21 is preferably 6 in the present embodiment.
Reaction cup card slot 21 is through-hole structure up and down, and the height of both ends positioning region 22 is greater than middle part reaction cup card slot
The height of 21 parts, and 22 upper and lower ends of positioning region protrude 21 end face of reaction cup card slot, i.e., reaction cup card slot 21 of middle part
Divide and is in overhead positions.
It is equipped with the transfer hole 22a of blind hole structure along the vertical direction on two positioning regions 22, send glass trolley 4 to transport to facilitate
A glass item is sent, 22 two sides of positioning region are equipped with the locating groove 22b of arc, and when cup item 2 is in above-mentioned channel, it is fixed to can use this
Position card slot 22b limits cup item 2.
In conjunction with Fig. 5 as can be seen that when reaction cup 1 is packed into cup item 2, upper end protrusion part and the reaction cup card slot of reaction cup 1
21 upper surfaces abut, and 21 lower end surface of reaction cup card slot is stretched out in 2 lower end of cup item, and oscillation is facilitated to mix.
Fig. 6 is that the cup item 2 equipped with reaction cup 1 is in the structural schematic diagram in respective channel, for convenience of describing, the present embodiment
By taking the B of reagent adding channel as an example, rest channels structure is approximate with its, is shape groove-like structure corresponding with 1 shape of cup item, but open
Slot number difference as needed, is provided along its length elastic slice 6 in the B outer rim of reagent adding channel, 6 end of elastic slice is solid
Surely there is limited block 6a, at least part structure on limited block 6a is corresponding to the locating groove 22b on cup item 2, when cup item 2 is put
When setting on the B of the reagent adding channel, limited block 6a is embedded in locating groove 22b, limits cup item 2 under the effect of elastic slice 6
Position.
Efficiency is filled to improve reagent, the reagent filling needle 5 in the present embodiment in reagent loading system is preferably 3, right
6 reaction cups on glass item 2 in 6 reaction cup card slots 21 are answered, it is excessive to also avoid system load while guaranteed efficiency.
Finally it is to be appreciated that foregoing description is merely a preferred embodiment of the present invention, those skilled in the art is in the present invention
Enlightenment under, without prejudice to the purpose of the present invention and the claims, multiple similar expressions, such change can be made
It changes and falls within the scope of protection of the present invention.
Claims (2)
1. a kind of magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method, which comprises the following steps:
S1: reaction cup (1) being sorted and is detachably loaded with respect in a glass item (2), is put into plus sample channel (A) is to fill detection sample
This；
S2: the cup item (2) after filling to be detected to sample is transported to reagent adding channel (B), and passes through reagent loading system to reaction
Detection reagent is filled in cup (1)；
S3: by the cup item (2) after filling detection reagent, pass through reagent and mix channel (C) progress reagent mixing；
S4: the cup item (2) after mixing is transported to incubation system (D), the detection sample in reaction cup (1) is incubated for；
S41: in the case of portion of reagent, after detecting sample incubation, corresponding cup item (2) is transported to reagent adding channel (B), is repeated
S2~S4 step；
S5: corresponding cup item (2) is transported to washing channel (E), washed to reaction cup (1) by detection sample after being incubated for；
S51: when using two-step method, after reaction cup (1) washing, corresponding cup item (2) is transported to reagent adding channel (B), is repeated
S2~S5 step；
S6: after reaction cup (1) washing, corresponding cup item (2) is transported to substrate filling and mixes channel (F) filling substrate and mixes
It is even；
S7: the corresponding cup item (2) after filling substrate is transported to detection and waits channel (G) etc. to be detected；
S8: it will test and the reaction cup (1) on channel (G) inner cup item (2) is waited to be grabbed one by one by handgrip (3) to detection system
(H) it is detected in；
And S9: the cup item (2) that will test the interior sky for having removed reaction cup (1) of waiting channel (G) is transported to plus sample channel
(A)；
Wherein, described plus sample channel (A), reagent adding channel (B) and washing channel (E) are at least two；
The cup item (2) is string configuration, and the reaction cup card slot (21) of at least one up and down is distributed with along its length,
The shape of the reaction cup card slot (21) is adapted with reaction cup (1) outer rim shape；
Cup item (2) both ends are equipped with positioning region (22), which protrudes reaction cup card slot (21) end
Face, the positioning region (22) are equipped with transfer hole (22a), and positioning region (22) two sides are equipped with the locating groove (22b) of arc；
The transport of the cup item (2) send a glass trolley (4) to carry out by least two；A glass trolley (4) are sent to send a glass trolley including first
(4a) and second is sent a glass trolley (4b), arranged in parallel, and step S2, S3, S51, S9 send a glass trolley (4a) independent by first
It completes, step S4, S5 individually send a glass trolley (4b) to complete by second, and step S6 and S7 are then by the control system of moving trolley (4)
Detection two is sent wherein one or two being in idle condition in a glass trolley (4) while being carried out, and step S41 first send cup small by second
Vehicle (4b) is transported to reagent and mixes channel (C), then send a glass trolley (4a) to be transported to reagent adding channel (B) by first；
Elastic slice (6) are provided along its length in reagent adding channel (B) outer rim, which is fixed with limited block (6a),
At least part structure on the limited block (6a) is corresponding to locating groove (22b) on cup item (2).
2. magnetic microparticle chemiluminescence immune assay instrument multicenter detecting method according to claim 1, it is characterised in that: institute
Stating reagent loading system includes at least one reagent filling needle (5).
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| CN201610127319.XA CN107167621B (en) | 2016-03-07 | 2016-03-07 | Multi-channel detection method for magnetic particle chemiluminescence immunoassay analyzer |
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| CN201610127319.XA CN107167621B (en) | 2016-03-07 | 2016-03-07 | Multi-channel detection method for magnetic particle chemiluminescence immunoassay analyzer |
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| CN107831325B (en) * | 2017-10-20 | 2021-02-26 | 厦门市波生生物技术有限公司 | Full-automatic chemiluminescence immunoassay analyzer |
| CN107664635A (en) * | 2017-11-17 | 2018-02-06 | 南通伊仕生物技术股份有限公司 | A kind of chemiluminescence detection reagent strip |
| CN108226549B (en) * | 2018-01-15 | 2021-07-13 | 重庆博奥新景医学科技有限公司 | Time sequence control method and system for chemiluminescence immunoassay analyzer |
| CN110082291B (en) * | 2018-01-25 | 2022-08-05 | 深圳市新产业生物医学工程股份有限公司 | Adsorption mechanism, cleaning device, chemiluminescence detector and cleaning method |
| CN108614101B (en) * | 2018-03-14 | 2021-04-06 | 嘉兴科瑞迪医疗器械有限公司 | Magnetic particle full-automatic chemiluminescence immunoassay analyzer |
| CN110346557B (en) * | 2019-07-15 | 2023-03-31 | 深圳海思安生物技术有限公司 | Detection kit |
| CN111413264B (en) * | 2020-04-02 | 2021-04-06 | 华中农业大学 | Multi-channel particle detection device and method for detecting micron particles |
| CN114487387B (en) * | 2022-01-24 | 2024-11-26 | 北京铂茵生物科技有限公司 | Electrochemical luminescence reaction cup, analysis method and optical detection device |
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