CN107158471A - A kind of preparation method of golden cypress compound bio glass tissue repair materials - Google Patents
A kind of preparation method of golden cypress compound bio glass tissue repair materials Download PDFInfo
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- CN107158471A CN107158471A CN201710405127.5A CN201710405127A CN107158471A CN 107158471 A CN107158471 A CN 107158471A CN 201710405127 A CN201710405127 A CN 201710405127A CN 107158471 A CN107158471 A CN 107158471A
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- golden cypress
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- repair materials
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- 241000218691 Cupressaceae Species 0.000 title claims abstract description 32
- 239000000463 material Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000017423 tissue regeneration Effects 0.000 title claims abstract description 12
- 239000005312 bioglass Substances 0.000 title claims abstract description 10
- 150000001875 compounds Chemical class 0.000 title claims abstract description 10
- 239000000284 extract Substances 0.000 claims abstract description 10
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 14
- 239000004626 polylactic acid Substances 0.000 claims description 14
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 238000005352 clarification Methods 0.000 claims description 3
- 230000001186 cumulative effect Effects 0.000 claims description 3
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 229920000428 triblock copolymer Polymers 0.000 claims description 3
- DQWPFSLDHJDLRL-UHFFFAOYSA-N triethyl phosphate Chemical class CCOP(=O)(OCC)OCC DQWPFSLDHJDLRL-UHFFFAOYSA-N 0.000 claims description 3
- 230000035939 shock Effects 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 230000007721 medicinal effect Effects 0.000 abstract description 2
- 238000009418 renovation Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 22
- 230000000694 effects Effects 0.000 description 6
- 239000011521 glass Substances 0.000 description 4
- 241000218202 Coptis Species 0.000 description 3
- 235000002991 Coptis groenlandica Nutrition 0.000 description 3
- WITLAWYGGVAFLU-UHFFFAOYSA-N 3-(6-methoxy-1,3-benzodioxol-5-yl)-8,8-dimethylpyrano[2,3-f]chromen-4-one Chemical group C1=CC(C)(C)OC2=CC=C(C(C(C3=CC=4OCOC=4C=C3OC)=CO3)=O)C3=C21 WITLAWYGGVAFLU-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000193449 Clostridium tetani Species 0.000 description 1
- 241000186227 Corynebacterium diphtheriae Species 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 241000186366 Mycobacterium bovis Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 241000972672 Phellodendron Species 0.000 description 1
- 241000607764 Shigella dysenteriae Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241001312524 Streptococcus viridans Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 206010047400 Vibrio infections Diseases 0.000 description 1
- 229910021417 amorphous silicon Inorganic materials 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 229940007046 shigella dysenteriae Drugs 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/112—Phosphorus-containing compounds, e.g. phosphates, phosphonates
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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Abstract
A kind of preparation method of golden cypress compound bio glass tissue repair materials, belongs to biological medicine Material Field, including：Repair materials are made in the preparation of cortex phellodendri extract, the preparation of PLA solution, the preparation of bio-vitric solution, electrostatic spinning.Wherein golden cypress has antibacterial action, and PLA can improve the mechanical performance of material, and bio-vitric can improve the medicinal properties of material.Tissue renovation material produced by the present invention has good mechanical performance, tissue repair performance and drug attachment.
Description
Technical field
The invention belongs to biological medicine Material Field, more particularly to a kind of golden cypress compound bio glass tissue repair materials
Preparation method.
Background technology
So far, people's more attention is influence of the bio-vitric component to bioactivity to self-discovery bio-vitric.Nanometer
The rise of technology, makes people start to recognize that structure also has a major impact bioactivity, more pays attention to dimensional effect, microcosmic
Influence of the fine structure to material structure and performance.Nanometer biological glass, mesoporous biological from nano-scale R ＆ D design
Glass is even more the concern for enjoying domestic and foreign scholars.Bio-vitric energy active cell gene, is conducive to strengthening the propagation of cell with dividing
Change, the effect with self-bone grafting and osteoacusis.But in view of the presence of its amorphous silicon skeleton, bio-vitric is still rendered as amorphous
State, bad mechanical property, and drug attachment is poor, its application on biological medicine material need further exploitation.
The content of the invention
In order to solve problems of the prior art, the invention provides a kind of good mechanical property, and with excellent
The preparation method of the golden cypress compound bio glass tissue repair materials of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of golden cypress compound bio glass tissue repair materials, comprises the following steps：
Step one：Golden cypress is cleaned, and is placed at aeration-drying and is air-dried, the golden cypress after air-drying is placed in 90
8min is dried under the conditions of DEG C, by dried golden cypress, is put into pulverizer and crushes, the golden cypress after crushing is crossed into 60 mesh sieves, obtains yellow
Bai Fen；
Step 2：2g golden cypress powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, under 40 DEG C of temperature conditionss
Constant temperature extracts 10h, and filter residue is taken out after solution is filtered, and 50ml absolute ethyl alcohol and the constant temperature under the conditions of 40 DEG C are added into filter residue
20min is extracted, filtrate will be merged twice after filtering again, and rotary evaporation is carried out under the conditions of 58 DEG C, cumulative volume is concentrated into
For 5ml, cortex phellodendri extract is obtained；
Step 3：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes,
24 hours are stirred at room temperature until PLA all dissolves, and are added the absolute ethyl alcohol of volume fraction 35%, are stirred at room temperature 24
Hour is uniform to solution clarification, obtains PLA solution；
Step 4：By 5g PEOs-PPOX-, that PEO triblock copolymer is dissolved in 62g volumes is dense
Spend in the ethanol solution for 70%, after being sufficiently stirred for, sequentially add 6g silester, 0.7g triethyl phosphates, 1.5gCa
(NO3)2·4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 5：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, biology is made
Glass solution；
Step 6：By PLA solution, cortex phellodendri extract, bio-vitric solution in mass ratio 50:2:8 are well mixed, and make
It is disperseed with ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir 8h at room temperature, obtain sticky molten
Liquid is placed in the medical needle tubing being connected with high-pressure installation, and repair materials are made by electrostatic spinning, and high-pressure installation voltage is 20kV,
Obtained material is dried in vacuo at 40 DEG C.
It is preferred that, the capacity of the medical needle tubing described in step 6 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
The beneficial effects of the present invention are：
1) PLA has good biodegradability, while also having good Bioabsorbable and bio-compatible
Property, any environmental issue will not be left after degradation, and in medical field, oneself is considered as most promising degradable macromolecule material
Material.
2) bio-vitric has good biocompatibility, and the function with tissue repair, and energy active cell gene has
Propagation and differentiation beneficial to enhancing cell, the effect with self-bone grafting and osteoacusis.
3) golden cypress has heat-clearing and damp-drying drug, purging intense heat, except steaming, effect of detoxification sore treatment.Golden cypress also has antibacterial action：Golden cypress
Antibiotic effective ingredient is jamaicin, therefore its pharmacological action is substantially similar to the coptis, but content is low compared with the coptis.In vitro test is to golden yellow
Color staphylococcus, pneumococcus, corynebacterium diphtheriae, Streptococcus viridans, shigella dysenteriae, hemolytic streptococcus, meningococcus, suddenly
Random vibrios, bacillus anthracis are effective or have stronger inhibitory action；Also have to hay bacillus, Bordetella pertussis, clostridium tetani
Inhibitory action；To H37Rv, mycobacterium tuberculosis without direct repression, but bacterium number can be reduced, but concentration up to 1% when present
Bacteriostasis.When making experimental therapy with cavy inoculation Mycobacterium hominis (H37Rv), oral or injection curative effect is very poor.It is right
The cavy of Mycobacterium bovis is inoculated with, during the hydrochloric acid crystal intramuscular injection of phellodendron extract, there is certain curative effect.Golden cypress is to tuberculosis
The clinical symptoms of people and x-ray inspection take a favorable turn, and better than the coptis.In test tube, golden cypress decoction or preserved material are to common pathogenic
Fungi has different degrees of bacteriostasis.
4) PLA can improve the mechanical performance of material, and bio-vitric can improve the medicinal properties of material.
5) material prepared using electrostatic spinning technique, with good porosity, is conducive to the attachment of medicine.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of golden cypress compound bio glass tissue repair materials, comprises the following steps：
Step one：Golden cypress is cleaned, and is placed at aeration-drying and is air-dried, the golden cypress after air-drying is placed in 90
8min is dried under the conditions of DEG C, by dried golden cypress, is put into pulverizer and crushes, the golden cypress after crushing is crossed into 60 mesh sieves, obtains yellow
Bai Fen；
Step 2：2g golden cypress powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, under 40 DEG C of temperature conditionss
Constant temperature extracts 10h, and filter residue is taken out after solution is filtered, and 50ml absolute ethyl alcohol and the constant temperature under the conditions of 40 DEG C are added into filter residue
20min is extracted, filtrate will be merged twice after filtering again, and rotary evaporation is carried out under the conditions of 58 DEG C, cumulative volume is concentrated into
For 5ml, cortex phellodendri extract is obtained；
Step 3：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes,
24 hours are stirred at room temperature until PLA all dissolves, and are added the absolute ethyl alcohol of volume fraction 35%, are stirred at room temperature 24
Hour is uniform to solution clarification, obtains PLA solution；
Step 4：By 5g PEOs-PPOX-, that PEO triblock copolymer is dissolved in 62g volumes is dense
Spend in the ethanol solution for 70%, after being sufficiently stirred for, sequentially add 6g silester, 0.7g triethyl phosphates, 1.5gCa
(NO3)2·4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 5：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, biology is made
Glass solution；
Step 6：By PLA solution, cortex phellodendri extract, bio-vitric solution in mass ratio 50:2:8 are well mixed, and make
It is disperseed with ultrasonic oscillation, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir 8h at room temperature, obtain sticky molten
Liquid is placed in the medical needle tubing being connected with high-pressure installation, and repair materials are made by electrostatic spinning, and high-pressure installation voltage is 20kV,
Obtained material is dried in vacuo at 40 DEG C.
The capacity of medical needle tubing described in step 6 is 20ml, and the syringe needle of the medical needle tubing described in step 6 is fixed on
At electrostatic spinning receiver board 20cm.
Claims (3)
1. a kind of preparation method of golden cypress compound bio glass tissue repair materials, it is characterised in that comprise the following steps：
Step one：Golden cypress is cleaned, and is placed at aeration-drying and is air-dried, the golden cypress after air-drying is placed in 90 DEG C of bars
8min is dried under part, by dried golden cypress, is put into pulverizer and crushes, the golden cypress after crushing is crossed into 60 mesh sieves, golden cypress powder is obtained；
Step 2：2g golden cypress powder is weighed, is placed in the wide-mouth bottle for filling 100ml absolute ethyl alcohols, the constant temperature under 40 DEG C of temperature conditionss
10h is extracted, filter residue is taken out after solution is filtered, 50ml absolute ethyl alcohol is added into filter residue and constant temperature is extracted under the conditions of 40 DEG C
20min, filtrate will merge twice after filtering again, and carry out rotary evaporation under the conditions of 58 DEG C, and being concentrated into cumulative volume is
5ml, obtains cortex phellodendri extract；
Step 3：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes, in room temperature
Lower stirring adds the absolute ethyl alcohol of volume fraction 35%, is stirred at room temperature 24 hours for 24 hours until PLA all dissolvings
It is uniform to solution clarification, obtain PLA solution；
Step 4：5g PEO-PPOX-PEO triblock copolymers are dissolved in into 62g volumetric concentrations is
In 70% ethanol solution, after being sufficiently stirred for, 6g silester, 0.7g triethyl phosphates, 1.5gCa (NO are sequentially added3)2·
4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 5：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, bio-vitric is made
Solution；
Step 6：By PLA solution, cortex phellodendri extract, bio-vitric solution in mass ratio 50:2:8 are well mixed, using super
Sound wave shock disperses to it, magnetic agitation 1h under the conditions of 50 DEG C, then continues to stir 8h at room temperature, obtains viscous solution and puts
In the medical needle tubing being connected with high-pressure installation, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, will be made
The material obtained is dried in vacuo at 40 DEG C.
2. a kind of preparation method of golden cypress compound bio glass tissue repair materials according to claim 1, its feature exists
In：The capacity of medical needle tubing described in step 6 is 20ml.
3. a kind of preparation method of golden cypress compound bio glass tissue repair materials according to claim 1, its feature exists
In：The syringe needle of medical needle tubing described in step 6 is fixed at electrostatic spinning receiver board 20cm.
Priority Applications (1)
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| CN109594195A (en) * | 2019-01-25 | 2019-04-09 | 蓝怡科技集团股份有限公司 | A kind of cotton shape fibrous framework and its preparation method and application |
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| CN104096261A (en) * | 2013-04-09 | 2014-10-15 | 上海交通大学医学院附属第九人民医院 | Gelatin / mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof |
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| CN104096261A (en) * | 2013-04-09 | 2014-10-15 | 上海交通大学医学院附属第九人民医院 | Gelatin / mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109594195A (en) * | 2019-01-25 | 2019-04-09 | 蓝怡科技集团股份有限公司 | A kind of cotton shape fibrous framework and its preparation method and application |
| CN109594195B (en) * | 2019-01-25 | 2021-11-05 | 蓝怡科技集团股份有限公司 | Cotton-shaped fiber scaffold and preparation method and application thereof |
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