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CN107114231A - A kind of selection of the red skin red meat color potato of precocious high nutrition - Google Patents

A kind of selection of the red skin red meat color potato of precocious high nutrition Download PDF

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CN107114231A
CN107114231A CN201710392919.3A CN201710392919A CN107114231A CN 107114231 A CN107114231 A CN 107114231A CN 201710392919 A CN201710392919 A CN 201710392919A CN 107114231 A CN107114231 A CN 107114231A
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potato
red
color
selection
skin
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陈勤
王冬冬
陈越
李哲清
马浩力
刘柏林
卢海斌
张小燕
赵华
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Northwest A&F University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

本发明公开了一种早熟高营养红皮红肉彩色马铃薯的选育方法,该方法包括以下步骤:通过有性杂交选用优质早熟高产适应性广的马铃薯品种与高含花青素、多酚等高抗氧化的马铃薯彩色品种杂交,经单株系统选育,无性世代多年田间评价,早期分离世代进行块茎皮和肉颜色的筛选及高代品系进行农艺性状、产量性状及成熟期等性状的筛选,而育成的农艺性状优良,富含抗氧化活性物质,成熟期在65天的红皮红肉的早熟马铃薯新品种。其品质优良,蒸食品味清香,口感细腻,干物质含量19.3%,淀粉含量13.6%,花青素含量高,多酚及抗氧化活性达到800‑1100mg/kg。本发明培育的马铃薯丰富了彩色马铃薯的品种,扩大了彩色马铃薯的种植区域。The invention discloses a method for breeding early-maturing, high-nutrition, red-skinned, red-fleshed, colored potatoes. The method comprises the following steps: selecting high-quality, early-maturing, high-yielding, and widely adaptable potato varieties with high anthocyanins, polyphenols, etc. through sexual crossbreeding. High-antioxidant potato color variety hybridization, single-plant system selection, multi-year field evaluation of asexual generations, screening of tuber skin and flesh color in early segregation generations, and screening of agronomic traits, yield traits, maturity and other traits of high-generation lines , and bred a new red-skinned red-fleshed early-maturing potato variety with excellent agronomic properties, rich in antioxidant active substances, and a maturity period of 65 days. Its quality is excellent, steamed food has a delicate fragrance, delicate taste, dry matter content of 19.3%, starch content of 13.6%, high anthocyanin content, polyphenols and antioxidant activity of 800‑1100 mg/kg. The potatoes cultivated by the invention enrich the varieties of colored potatoes and expand the planting area of colored potatoes.

Description

一种早熟高营养红皮红肉彩色马铃薯的选育方法Breeding method for early-maturing high-nutrition red-skinned red-fleshed colored potatoes

技术领域technical field

本发明属于农业技术领域,具体地说,涉及一种早熟高营养红皮红肉彩色马铃薯的选育方法。The invention belongs to the technical field of agriculture, and in particular relates to a breeding method for early-maturing high-nutrition red-skinned red-fleshed colorful potatoes.

背景技术Background technique

马铃薯是世界上重要粮食作物,排在小麦、水稻、玉米之后位居第四。而我国马铃薯种植面积和总产量居世界第一,在粮食安全中具有重要的战略地位。从世界马铃薯育成品种来看,多数为普通的白色或黄色品种,红皮红肉很少,市场几乎见不到。我国彩色马铃薯的研究刚刚起步,现有市场上见到的个别彩色马铃薯多数为紫色引进品种,原创具有自主知识产权的品种极少。而现有国外引进的彩色马铃薯均为成熟期长的晚熟品种。外来的品种抗逆性不强,品种适应性差,产量极低,不能满足国内市场和生产的需要,因此亟需选育一批具有自主知识产权,品质好、产量高,成熟期早,适应我国不同生态区种植的优质彩色马铃薯新品种。Potato is an important food crop in the world, ranking fourth after wheat, rice and corn. However, my country's potato planting area and total output rank first in the world, and it has an important strategic position in food security. Judging from the varieties of potatoes bred in the world, most of them are ordinary white or yellow varieties, with very few red skin and red meat, and they are hardly seen in the market. The research on colored potatoes in my country has just started. Most of the individual colored potatoes seen in the market are purple imported varieties, and there are very few original varieties with independent intellectual property rights. And the color potato that existing foreign country introduces all is the late-maturing variety with long maturation period. Foreign varieties are not strong in stress resistance, poor in adaptability, and extremely low in yield, which cannot meet the needs of the domestic market and production. Therefore, there is an urgent need to breed a batch of species with independent intellectual property rights, good quality, high yield, and early maturity, which are suitable for our country. New varieties of high-quality colored potatoes grown in different ecological zones.

发明内容Contents of the invention

本发明的目的在于提供一种早熟红皮红肉彩色马铃薯的育种方法,该方法是通过品种间有性杂交育种,单株系统选育,无性世代多年田间评价而育成的一种早熟、优质,薯肉为红色,成熟期在65天左右,花青素含量高达265mg/kg,多酚含量高达826mg/kg鲜重,抗氧化活性达到1080mg/kg,适合花青素及全粉加工的红皮红肉彩色马铃薯的育种方法。The purpose of the present invention is to provide a kind of breeding method of early-maturing red-skinned red-fleshed colorful potato, which is a kind of early-maturing, high-quality potato which is bred through sexual cross breeding between varieties, single-plant system selection, and multi-year field evaluation of asexual generations. The potato flesh is red, the maturity period is about 65 days, the anthocyanin content is as high as 265mg/kg, the polyphenol content is as high as 826mg/kg fresh weight, and the antioxidant activity is 1080mg/kg. It is suitable for anthocyanin and whole powder processing red skin Breeding methods of red-fleshed colored potatoes.

其具体技术方案为:Its specific technical plan is:

一种早熟高营养红皮红肉彩色马铃薯的选育方法,包括以下步骤:A method for breeding early-maturing high-nutrition red-skinned red-fleshed colored potatoes, comprising the following steps:

步骤1、选用一个综合性状优良的红皮红肉的马铃薯株系ACP1851作为母本和一个红土豆株系ACP1691作为父本进行杂交,构建彩色马铃薯杂交育种分离群体。Step 1. Select a red-skinned and red-fleshed potato line ACP1851 with excellent comprehensive properties as the female parent and a red potato line ACP1691 as the male parent for crossing to construct a color potato hybrid breeding segregation population.

步骤2、G0和G1中的早期薯块颜色选择及鉴定;将配制的杂交组合马铃薯种子播种于温室的花盆中,播种60天左右,单株收获小薯,冲洗去除泥土,取小薯切开,考察薯皮及薯肉颜色。Step 2. Color selection and identification of early tubers in G0 and G1; sow the prepared hybrid combination potato seeds in flower pots in the greenhouse for about 60 days, harvest small tubers from a single plant, wash and remove the soil, take the tubers and cut them into pieces. Open, inspect the color of potato skin and potato flesh.

步骤3、G1世代进行多点多地成熟期及农艺性状选择。G1世代重点筛选成熟期在40-80天的株系,而对其颜值不深,但农艺形状好或颜值高的可再进行回交。Step 3, the G1 generation is selected for maturity stage and agronomic traits in multiple locations. In the G1 generation, the focus is on screening the lines with a maturity period of 40-80 days, and the lines that are not deep in appearance but have good agronomic shape or high appearance can be backcrossed.

步骤4、G2入选单株进行田间综合鉴定,选择株型适中,结薯性好,产量高,颜色好,品质优良,成熟期在40-80天,综合性状优良的单株,进行第三年株系评选。Step 4, G2 selected individual plants for comprehensive identification in the field, select individual plants with moderate plant type, good tuber formation, high yield, good color, good quality, maturity period of 40-80 days, and excellent comprehensive properties, and carry out the third year Strain selection.

步骤5、综合评价鉴定:在育种选育田对入选品系进行产量、农艺性状、品质及薯块颜色进行鉴定,获得综合性状优良的彩色高营养红皮红肉新品系,G3对入选株系/品系进行品质、花青素含量,抗氧化活性的测定。Step 5. Comprehensive evaluation and identification: In the breeding and selection field, identify the yield, agronomic traits, quality and color of potato pieces of the selected strains, and obtain a new color and high-nutrition red-skinned red meat strain with excellent comprehensive properties. The strains were tested for quality, anthocyanin content, and antioxidant activity.

进一步,步骤2中所述G0世代从红皮红肉、紫皮白肉、红皮白肉、浅红、深红等千余株不同类型中选择薯皮薯肉为全红的个体,选育出椭圆形红皮红肉,皮色与肉色一致,薯形整齐一致的早熟高营养红皮红肉彩色马铃薯。Further, the G0 generation described in step 2 selects individuals with red skin and potato flesh from more than a thousand plants with red skin and red meat, purple skin and white meat, red skin and white meat, light red, dark red, etc. Shaped red-skinned red-fleshed potatoes with the same skin color and flesh-colored, early-maturing high-nutrition red-skinned red-fleshed colored potatoes with uniform potato shape.

进一步,所培育的早熟彩色马铃薯为红皮红肉、薯形为圆与椭圆或长椭圆。叶茎粗度为0.6-1.4cm,株高50-60cm,花青素含量265mg/kg鲜重,多酚含量800-1000mg/kg鲜重,其花青素含量比普通对照品种高3倍以上,抗氧化活性达到1080mg/kg。Further, the cultivated early-maturing colored potatoes are red-skinned and red-fleshed, and the potato shape is round, ellipse or long ellipse. The leaf and stem thickness is 0.6-1.4cm, the plant height is 50-60cm, the anthocyanin content is 265mg/kg fresh weight, the polyphenol content is 800-1000mg/kg fresh weight, and the anthocyanin content is more than 3 times higher than that of the common control variety , the antioxidant activity reached 1080mg/kg.

进一步,所培育的早熟红皮红肉彩色马铃薯成熟期在65天左右,比普通对照品种提前30天以上,单株结薯数5-15粒,单薯重在50g-200g。Furthermore, the mature period of the cultivated early-maturing red-skinned red-fleshed colored potatoes is about 65 days, which is more than 30 days earlier than that of the common control variety. The number of potatoes per plant is 5-15, and the weight of a single potato is 50g-200g.

与现有技术相比,本发明的有益效果:Compared with prior art, the beneficial effect of the present invention:

本发明通过选用优质高产的马铃薯品系分别与高含花青素、多酚等高抗氧化的马铃薯彩色品种杂交,经早期分离世代进行块茎皮和肉颜色的筛选及高代品系进行农艺性状、产量性状及成熟期等性状的筛选,而育成的农艺性状优良,富含抗氧化活性物质,成熟期在60-70天的红皮红肉的早熟马铃薯新品种。本发明培育的早熟红皮红肉马铃薯极大丰富了彩色马铃薯的品种,扩大了彩色马铃薯的种植区域。In the present invention, high-quality and high-yield potato strains are respectively hybridized with high-anthocyanidin, polyphenol and other high-antioxidant potato color varieties, and the screening of tuber skin and meat color is carried out through early separation generations, and the agronomic traits and yield of high-generation strains are selected. Characters and maturity period screening, and bred a new early-maturing potato variety with red skin and red flesh that has excellent agronomic properties, rich in antioxidant active substances, and a maturity period of 60-70 days. The early-maturing red-skinned and red-fleshed potatoes cultivated by the invention greatly enrich the variety of colored potatoes and expand the planting area of colored potatoes.

具体实施方式detailed description

下面结合具体实施方案对本发明的技术方案作进一步详细地说明。The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

一种早熟高营养红皮红肉彩色马铃薯的选育方法,包括以下步骤:A method for breeding early-maturing high-nutrition red-skinned red-fleshed colored potatoes, comprising the following steps:

步骤1、选用一个综合性状优良的红皮红肉的马铃薯株系ACP1851作为母本和一个红土豆株系ACP1691作为父本进行杂交,构建彩色马铃薯杂交种分离群体;Step 1, select a red-skinned and red-fleshed potato line ACP1851 with excellent comprehensive properties as the female parent and a red potato line ACP1691 as the male parent for hybridization to construct a color potato hybrid segregation population;

步骤2、对G0世代进行早期的薯块颜色选择及鉴定,将配制的杂交组合马铃薯种子播种于温室的花盆中,单株收获小薯,冲洗去除泥土,取小薯切开,考察薯皮及薯肉颜色;Step 2. Carry out early color selection and identification of potato pieces in the G0 generation, sow the prepared hybrid combination potato seeds in flower pots in the greenhouse, harvest small potatoes from individual plants, wash and remove the soil, cut the small potatoes, and inspect the potato skins and potato meat color;

步骤3、G1世代进行多点多地成熟期及农艺性状选择;G1世代重点筛选成熟期在40-80天的株系,而对其颜值不深,但农艺形状好或颜值高的再进行回交;Step 3. The G1 generation selects the maturation period and agronomic traits in multiple locations and locations; the G1 generation focuses on screening the strains with a maturity period of 40-80 days, and the lines that are not deep in appearance but have good agronomic shape or high appearance. make a backcross;

步骤4、G2入选单株进行田间综合鉴定,选择株型适中,结薯性好,产量高,颜色好,品质优良,成熟期在50-70天,综合性状优良的单株,进行第三年株系评选;Step 4: G2 selected individual plants for comprehensive identification in the field, select individual plants with moderate plant type, good tuber formation, high yield, good color, good quality, maturity period of 50-70 days, and excellent comprehensive properties, and carry out the third year strain selection;

步骤5、综合评价鉴定:对G3在育种选育田对入选品系进行产量、农艺性状、品质及薯块颜色进行鉴定,获得综合性状优良的彩色高营养新品系,对G3入选株系/品系进行品质、花青素含量,抗氧化活性的测定。Step 5. Comprehensive evaluation and identification: Identify the yield, agronomic traits, quality and tuber color of the selected strains of G3 in the breeding and selection field to obtain a new color and high-nutrition strain with excellent comprehensive properties. Determination of quality, anthocyanin content, and antioxidant activity.

步骤2中所述的对G0世代进行早期的薯块颜色选择及鉴定,播种60天后即开展薯块早期颜色筛选,在杂交后代中从红皮红肉、紫皮白肉、红皮白肉、浅红、深红的不同类型中选择薯皮薯肉皮色与肉色一致为全红的个体。Carry out early color selection and identification of potato cubes for the G0 generation described in step 2, and carry out early color screening of potato cubes 60 days after sowing. Among the different types of dark red and dark red, choose the individual whose skin color and flesh color are all red.

步骤3中所述的G1世代进行多点多地成熟期及农艺性状选择,具体为:育的新品系块茎圆至卵圆形,红皮红肉,表皮光滑,单薯重在50g-200g,整齐;芽眼多而浅,结薯集中;半光生幼芽基部窄圆柱形,深红色;顶部钝形,红紫色,茸毛多。The G1 generation described in step 3 is selected for multi-point maturity and agronomic traits, specifically: the new line of breeding has round to oval tubers, red skin and red meat, smooth skin, and the weight of a single potato is 50g-200g. Neat; many and shallow bud eyes, concentrated tubers; semi-glossy young buds with narrow cylindrical base, dark red; blunt top, reddish purple, with many hairs.

步骤5中所述的对G3在育种选育田对入选品系进行产量、农艺性状、品质及薯块颜色进行鉴定,具体为:选育的新品系农艺性状株型开展、分支少;株高50-60厘米;茎绿带紫色;叶绿色,茸毛多,复叶较大,叶缘平展,卵圆形,侧小叶3-4对;花冠白色;植株中抗晚疫病,块茎易感染花叶病毒病和卷叶病毒病;极抗旱,耐贫瘠;单株结薯数5-15粒,单薯重在50g-200g;亩产1500-3000公斤。In step 5, the G3 is identified in the breeding and breeding field for the yield, agronomic traits, quality and tuber color of the selected strains, specifically: the agronomic traits of the selected strains have developed plant type and few branches; the plant height is 50 -60 cm; stem green with purple; leaves green, hairy, large compound leaves, leaf margins flat, oval, 3-4 pairs of side leaflets; corolla white; plants resistant to late blight, tubers susceptible to mosaic virus disease and leaf curl virus disease; extremely drought-resistant and barren-resistant; the number of tubers per plant is 5-15, and the weight of a single tuber is 50g-200g; the yield per mu is 1500-3000 kg.

步骤5中所述的对G3入选株系/品系进行品质、花青素含量,抗氧化活性的测定,具体为:选育的新品系蒸食品味清香,口感细腻;干物质含量19.3%,淀粉含量13.6%,花青素含量265mg/kg鲜重,多酚含量826mg/kg鲜重,其花青素含量比普通对照品种高3倍以上,抗氧化活性物质含量达到1080mg/kg。The determination of the quality, anthocyanin content and antioxidant activity of the selected strains/strains of G3 described in step 5 is specifically: the steamed food of the selected new strains has a delicate taste and delicate taste; the dry matter content is 19.3%, and the starch content is 19.3%. 13.6%, the anthocyanin content is 265mg/kg fresh weight, the polyphenol content is 826mg/kg fresh weight, the anthocyanin content is more than 3 times higher than that of the common control variety, and the antioxidant active substance content reaches 1080mg/kg.

本发明所培育的早熟红皮红肉彩色马铃薯成熟期在65天左右,比普通对照品种提前30天以上,单株结薯数5-15粒,单薯重在50g-200g。The ripening period of the early-maturing red-skinned red-fleshed colorful potatoes cultivated by the invention is about 65 days, which is more than 30 days earlier than that of common control varieties. The number of potatoes per plant is 5-15, and the weight of a single potato is 50g-200g.

以上所述,仅为本发明较佳的具体实施方式,本发明的保护范围不限于此,任何熟悉本技术领域的技术人员在本发明披露的技术范围内,可显而易见地得到的技术方案的简单变化或等效替换均落入本发明的保护范围内。The above is only a preferred specific embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Any person familiar with the technical field within the technical scope disclosed in the present invention can obviously obtain the simplicity of the technical solution. Changes or equivalent replacements all fall within the protection scope of the present invention.

Claims (5)

1. a kind of selection of the red skin red meat color potato of precocious high nutrition, it is characterised in that including following operating procedure:
Step 1, the potato strain ACP1851 from the excellent red skin red meat of a Comprehensive Traits are used as a maternal and laterite Beans strain ACP1691 is hybridized as male parent, builds color potato cenospecies segregating population;
Step 2, the selection of potato wedge color and identification for carrying out early stage from generation to generation to G0, the cross combination potato seed of preparation is sowed In the flowerpot in greenhouse, individual plant harvests small potato, washes off soil, takes small potato to cut, and investigates potato skin and potato meat color;
Step 3, G1 carry out multiple spot many ground maturity period and economical character selection from generation to generation;G1 generations emphasis screens the maturity period in 40-80 It strain, and not deep to its face value, but agronomic shape is good or face value is high is returned again;
Step 4, G2 are selected in individual plant and carry out field comprehensive identification, and selection plant type is moderate, and knot potato is good, and yield is high, and color is good, quality Excellent, the maturity period, the excellent individual plant of Comprehensive Traits carried out strain competition in the 3rd year at 50-70 days;
Step 5, overall merit identification:Yield, economical character, quality and potato wedge are carried out to selected strains in breeding seed selection field to G3 Color is identified, obtains the excellent colored high nutrition new lines of Comprehensive Traits, and being selected in strain/strain to G3 carries out quality, flower Blue or green cellulose content, the measure of antioxidation activity.
2. the selection of the red skin red meat color potato of precocity high nutrition as claimed in claim 1, it is characterised in that step The potato wedge color for carrying out early stage from generation to generation to G0 described in 2 selects and identified that sowing carries out potato wedge early stage color sieve after 60 days Choosing, selects potato skin potato meat in the red skin red meats of Dai Zhongcong, purple skin plain boiled pork, red skin and white flesh, pale red, dark red different type after hybridization Color of the leather is consistent for complete red individual with yellowish pink.
3. the selection of the red skin red meat color potato of precocity high nutrition as claimed in claim 1, it is characterised in that step G1 described in 3 carries out multiple spot many ground maturity period and economical character selection from generation to generation, is specially:The new lines stem tuber circle educated is to oval Shape, red skin red meat, epidermis is smooth, and single potato focuses on 50g-200g, neatly;Of more buds and shallow, knot potato is concentrated;Half photoproduction young shoot base portion Narrow cylinder, peony;Top obtuse, reddish violet, fine hair is more.
4. the selection of the red skin red meat color potato of precocity high nutrition as claimed in claim 1, it is characterised in that step Being identified in breeding seed selection field selected strains progress yield, economical character, quality and potato wedge color G3 described in 5, Specially:The new lines economical character plant type of seed selection is carried out, branch is few;50-60 centimetres of plant height;Stem greenbelt purple;Leaf green, it is fine and soft Mao Duo, compound leaf is larger, and leaf margin is open and flat, oval, 3-4 pairs of side leaflet;Corolla white;Plant moderate resistance late blight, stem tuber easy infection Mosaic virus and leaf curl viral disease;Pole drought resisting, impoverishment tolerant;Individual plant knot potato number 5-15, single potato focuses on 50g-200g;Per mu yield 1500-3000 kilograms.
5. the selection of the red skin red meat color potato of precocity high nutrition as claimed in claim 1, it is characterised in that step Strain/the strain that is selected in G3 described in 5 carries out quality, anthocyanidin content, and the measure of antioxidation activity is specially:Seed selection New lines steamed wheaten foods samples delicate fragrance, delicate mouthfeel;Dry matter content 19.3%, content of starch 13.6%, anthocyanidin content 265mg/ Kg fresh weights, polyphenol content 826mg/kg fresh weights, its anthocyanidin content is higher than normal control kind more than 3 times, antioxidation activity thing Matter content reaches 1080mg/kg.
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CN116965332A (en) * 2023-09-05 2023-10-31 浙江大学 Breeding method for potatoes with both appreciation and eating functions

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Publication number Priority date Publication date Assignee Title
CN107806930A (en) * 2017-10-08 2018-03-16 内蒙古自治区农牧业科学院 A method for quickly judging the color of potato skin and potato flesh in the field
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CN114431136B (en) * 2022-02-15 2023-02-07 浙江大学 A method for breeding dual-purpose high-resistant starch potatoes
CN116724884A (en) * 2023-07-27 2023-09-12 上海浙江大学高等研究院 Breeding method of double-harvest potatoes with solanaceous fruit and potato blocks
CN116965332A (en) * 2023-09-05 2023-10-31 浙江大学 Breeding method for potatoes with both appreciation and eating functions
CN116965332B (en) * 2023-09-05 2024-10-25 浙江大学 A breeding method for potatoes that are both edible and palatable

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