CN107064484A - Self-priming Dry-type biochemical detection means - Google Patents
Self-priming Dry-type biochemical detection means Download PDFInfo
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- CN107064484A CN107064484A CN201710388858.3A CN201710388858A CN107064484A CN 107064484 A CN107064484 A CN 107064484A CN 201710388858 A CN201710388858 A CN 201710388858A CN 107064484 A CN107064484 A CN 107064484A
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- bottom plate
- detection means
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- type biochemical
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 238000012360 testing method Methods 0.000 claims abstract description 41
- 230000000717 retained effect Effects 0.000 claims abstract 2
- 238000006243 chemical reaction Methods 0.000 claims description 15
- 239000011248 coating agent Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000007788 liquid Substances 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 229920006267 polyester film Polymers 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 1
- 108010082126 Alanine transaminase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108010023302 HDL Cholesterol Proteins 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention provides a kind of self-priming Dry-type biochemical detection means, including：Upper cover and bottom plate, the left and right sides of the bottom plate is provided with limited post, and the upper lid be may be detachably retained between the limited post of the bottom plate left and right sides, and test paper is provided between the contact surface of base portion and the bottom plate on described；The bottom plate is provided with the groove for placing test paper between the limited post of the left and right sides, and is provided with least one detection hole below the groove, and each detection hole is separate and has predetermined interval；The top of upper lid is provided with sample holes, and bottom is provided with sample outlet hole, is turned between sample holes and sample outlet hole by a flow-guiding channel.The present invention can realize that automatic ration draws sample to be measured, once test multiple projects and be loaded without extra use pipettor or suction pipe.
Description
Technical field
The present invention relates to biochemistry detection field, and in particular to a kind of self-priming Dry-type biochemical detection means.
Background technology
The sample sample-adding of existing Dry-type biochemical detector bar is more to be transferred to sample to be detected with pipettor or quantitative straw
Extra configuration pipettor or suction pipe are needed in the sample application region of Dry-type biochemical detector bar, during Samples detection, absorption is had during operation
Sample and the step of sample is transferred into test-strips, and there is pollution problem that pipette tips or suction pipe cause and manual operation is improper draws
The inaccurate caused detection error of sample-adding amount risen.It is to be measured with quantitative absorption the present invention relates to a kind of Test paper device
Sample is loaded without extra use pipettor or suction pipe, solves the behaviour using pipettor or suction pipe when Test paper is detected
The sample pollution of work and operating procedure the problem of sample-adding amount accuracy and when reducing detection.
At present, drying chemical reagent paper detection method, which is usually used in determining in quantitative or qualitative test liquid sample, whether there is certain
Glucose in composition, such as blood sample, cholesterol, glutamic-pyruvic transaminase etc..The foundation of Dry-type biochemical detector bar can disposably be surveyed
Fixed sample size and the difference of test index species, are divided into measure individual event and multipurpose test paper.Its general method of testing is to use
The sample to be detected of specified quantitative is added dropwise in the sample application region of bar by pipettor or quantitative straw, within a certain period of time visual observation
Or test out result using necessary instrument.The individual event test paper as disclosed in United States Patent (USP) US7087397 is used to determine blood
In HDL-C.Device disclosed in this patent has the upper plate of well and the lower plate with testing window,
Test paper is pressed against between the upper and lower plates.The detection means needs to use liquid-transfering gun or sample-adding pipe, and once sample-adding can only be surveyed
Try single project.US7494818, which discloses once sample-adding, just can detect the dry chemical detection means of multiple projects.Add including one
The sample of dropwise addition, is evenly distributed to multiple by sample hole and multiple test blocks using the Distribution Layer between well and multiple test blocks
On reagent block, so as to realize that once sample-adding completes the function of multiple detection projects.The detection means still need using liquid-transfering gun or
Sample-adding pipe is loaded.
A kind of detection means, including upper lid are disclosed in CN102680672A, the bottom plate that is combined is covered and positioned at upper with upper
Test paper between lid and bottom plate.On cover at lower surface be provided with flow-guiding structure, in the well, flow-guiding structure, survey of upper lid
Have a try paper coating and detection zone between form fluid transfer, so as to realize the once multiple projects of sample-adding test.Should
Detection means stills need to be loaded using liquid-transfering gun or sample-adding pipe.
Disclosed in CN106198950A it is a kind of can realize automatic ration sample-adding detection means, including the first cover plate and
Control amount Loading channel is provided with second cover plate, the first cover plate.Include adding mouth and liquid outlet on the control amount Loading channel, extremely
It is few to include one section of capillary channel near adding mouth.There is sample-adding platform on first cover plate, adding mouth is located at sample-adding platform center.Sample-adding
When liquid sample by the capillary channel near adding mouth enter adding mouth in control amount Loading channel.Control amount Loading channel
Volume determines the volume of sample-adding.The more complicated needs of the detection means design increase sample-adding platform and extra capillary on device
Tube passage.Sample can produce residual in sample-adding platform and capillary channel during sample-adding causes actual sampling amount increase, is difficult to realize
Multinomial detection, operator can not also be visually observed that whether the sample inside Loading channel is enough.
The content of the invention
The invention provides a kind of self-priming Dry-type biochemical detection means, it is possible to achieve automatic ration draws sample to be measured,
Multiple projects are once tested to be loaded without extra use pipettor or suction pipe.Test device need not increase sample-adding platform with
And extra capillary channel, it is to avoid sample is remained.Operator can directly observe the sample state inside Loading channel, it is to avoid
The result that specimen amount deficiency is produced occurs postponing or mistake, and concrete scheme is as follows：
A kind of self-priming Dry-type biochemical detection means, including：
Upper cover and bottom plate, the left and right sides of the bottom plate is provided with limited post, and the upper lid is detachably secured
Between the limited post of the bottom plate left and right sides, test is provided between the contact surface of base portion and the bottom plate on described
Test paper；
The bottom plate is provided with the groove for placing test paper between the limited post of the left and right sides, and in the groove
Lower section is provided with least one detection hole, and each detection hole is separate and with predetermined interval；
The top of upper lid is provided with sample holes, and bottom is provided with sample outlet hole, is led between sample holes and sample outlet hole by one
Circulation road is turned on.
Further, the sample holes are raised in the upper caping end surfaces.
Further, the flow-guiding channel is the transparent cavity through the upper lid whole thickness.
Further, the length of the flow-guiding channel is 3-30 millimeters, and volume is 3-200 microlitres.
Further, the flow-guiding channel selects the material for including hydrophilic substance to be made, or the flow-guiding channel is surface
By the transparent material of hydrophilic treated.
Further, the test paper includes the coating at top and an at least reaction block for bottom, each reaction block phase
Mutual independence and there is predetermined interval；
The coating is contacted with the sample outlet hole, and the position of each reaction block is just to each detection hole.
Further, the section of sample holes, sample outlet hole and flow-guiding channel is shaped as circular, polygon or elliptical shape.
The technical scheme provided using the present invention, with quantitative absorption sample to be measured without extra use liquid relief
Device or suction pipe sample-adding, the sample of operation when solving Test paper detection using pipettor or suction pipe pollute and sample-adding amount accuracy
The problem of and operating procedure when reducing detection.
Brief description of the drawings
Technical scheme in order to illustrate the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art
In required for the accompanying drawing that uses be briefly described, it should be apparent that, drawings in the following description are only some of the present invention
Embodiment, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these
Accompanying drawing obtains other accompanying drawings.
A kind of exploded perspective view for self-priming each part of Dry-type biochemical detection means that Fig. 1 provides for the present invention；
A kind of schematic diagram of the combination for self-priming Dry-type biochemical detection means that Fig. 2 provides for the present invention；
Fig. 3-4 is the schematic diagram covered in the present invention；
Fig. 5-6 is the schematic diagram of bottom plate of the present invention.
Embodiment
In the following description, a large amount of concrete details are given to provide more thorough understanding of the invention.So
And, it is obvious to the skilled person that the present invention can be able to without one or more of these details
Implement.In other examples, in order to avoid obscuring with the present invention, do not enter for some technical characteristics well known in the art
Row description.
In order to thoroughly understand the present invention, detailed step and detailed structure will be proposed in following description, so as to
Explain technical scheme.Presently preferred embodiments of the present invention is described in detail as follows, but in addition to these detailed descriptions, this
Invention can also have other embodiment.
Shown in reference picture 1- Fig. 6, the invention provides a kind of self-priming Dry-type biochemical detection means, including：
Upper lid 10 and bottom plate 20, the left and right sides of bottom plate 20 are provided with limited post 21, and upper lid 10 is removably solid
Between the limited post 21 for being scheduled on the left and right sides of bottom plate 20, test examination is provided between the upper bottom of lid 10 and the card access surface of bottom plate 20
Paper；
Bottom plate is provided with the groove 25 for placing test paper between the limited post 21 of 20 left and right sides, and recessed at this
The lower section of groove 25 is provided with least one detection hole 22, and each detection hole 22 is separate and with predetermined interval.Pass through detection zone
In test block phase mutual interval necessarily, prevent the cross pollution between test block；
The top of upper lid 10 is provided with sample holes 12, and bottom is provided with sample outlet hole 14, sample holes 12 and sample outlet hole 14 it
Between pass through a flow-guiding channel (or microchannel) 11 turn on.
In an optional embodiment of the invention, the internal cavity surface of flow-guiding channel 11 is hydrophilic material or includes parent
Water substance.Liquid is diffused in microchannel by capillarity, and the cavity content volume of microchannel determines product
Sample-adding amount.In optional embodiment, the length of flow-guiding channel 11 is 3-30 millimeters, and volume is 3-200 microlitres.
In an optional embodiment of the invention, the sample holes are raised in the upper caping end surfaces.
In an optional embodiment of the invention, flow-guiding channel 11 is the transparent cavity through the upper whole thickness of lid 10.
In an optional embodiment of the invention, flow-guiding channel 11 is made from the material for including hydrophilic substance, or this is led
Circulation road can be transparent material of the surface Jing Guo hydrophilic treated.
In an optional embodiment of the invention, as shown in figure 1, coating 30 and bottom of the test paper including top
An at least reaction block 40, each reaction block 40 is separate and has predetermined interval；The coating is contacted with sample outlet hole 14, in order to
Liquid in flow-guiding channel 11 is transmitted to coating 30, the position of each reaction block 40 is just to each detection hole 22, reaction block 22
It is to be covered in the detection hole 22 of the lower section of groove 25, is easy to instrument to be detected by 22 pairs of each reaction blocks 40 of detection hole.Its
In, the material that coating 30 is used is to absorb any material of liquid sample, such as gauze, filter paper, glass, polyester film,
Non-woven fabrics etc., preferably polyester film.
In an optional embodiment of the invention, the detection zone that each detection hole 22 is constituted close to the side of sample outlet hole 14,
Liquid in microchannel is conducive to preferably to infiltrate into test paper.
In the optional embodiment of the invention, the section of sample holes 12, sample outlet hole 14 and flow-guiding channel 11 is shaped as
The other shapes such as circular, polygon or elliptical shape.
When the self-priming Dry-type biochemical detection means provided using the present invention is detected, liquid sample touches detection dress
Put after the well of lid, drawn sample into microchannel by capillarity, then sample passes through sample outlet hole and reaches test
The coating 30 of test paper, and sample is diffused to by one or more bottoms of coating 30 by the diffusion of the material of coating 30
Reaction block 40, the color change of reaction block 40 is obtained a result in the range of the detection aperture that instrument passes through bottom base plate, is facilitated
Testing staff is detected and sampled to multiple detection zones.
Test paper is placed in the groove 25 of bottom plate 20, and keeps the coating 30 of test paper upward, bottom it is anti-
Answer block 40 just to each detection hole 22, afterwards block upper lid 10 between limited post 21 on the base plate 20.Drawn by sample holes 12
Sample to be tested, sample to be tested flows through flow-guiding channel 11 and the coating 30 on test paper surface is penetrated into by sample outlet hole 14.Treat
Test sample sheet reaches each reaction block 40 of bottom after the scattering and permeating of coating 30.Chemically react the color of generation in reaction block
Change can be detected by necessary instrument.
The present invention can realize that automatic ration draws sample to be measured, once test multiple projects without extra use
Pipettor or suction pipe sample-adding.Test device need not increase sample-adding platform and extra capillary channel, it is to avoid sample is remained.Behaviour
Author can directly observe the sample state inside Loading channel, it is to avoid the result that specimen amount deficiency is produced occurs postponing or wrong
By mistake.
Presently preferred embodiments of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, wherein the equipment and structure be not described in detail to the greatest extent are construed as giving reality with the common mode in this area
Apply；Any those skilled in the art, without departing from the scope of the technical proposal of the invention, all using the disclosure above
Methods and techniques content make many possible variations and modification to technical solution of the present invention, or be revised as equivalent variations etc.
Embodiment is imitated, this has no effect on the substantive content of the present invention.Therefore, every content without departing from technical solution of the present invention, foundation
The technical spirit of the present invention still falls within the present invention to any simple modifications, equivalents, and modifications made for any of the above embodiments
In the range of technical scheme protection.
Claims (7)
1. a kind of self-priming Dry-type biochemical detection means, it is characterised in that including：
Upper cover and bottom plate, the left and right sides of the bottom plate is provided with limited post, and the upper lid may be detachably retained at institute
Between the limited post for stating the bottom plate left and right sides, test examination is provided between the contact surface of base portion and the bottom plate on described
Paper；
The bottom plate is provided with the groove for placing test paper between the limited post of the left and right sides, and below the groove
At least one detection hole is provided with, each detection hole is separate and with predetermined interval；
The top of upper lid is provided with sample holes, and bottom is provided with sample outlet hole, logical by a water conservancy diversion between sample holes and sample outlet hole
Road is turned on.
2. self-priming Dry-type biochemical detection means as claimed in claim 1, it is characterised in that the sample holes are raised in described
Upper caping end surfaces.
3. self-priming Dry-type biochemical detection means as claimed in claim 1, it is characterised in that the flow-guiding channel is through institute
State the transparent cavity of lid whole thickness.
4. self-priming Dry-type biochemical detection means as claimed in claim 1, it is characterised in that the length of the flow-guiding channel is
3-30 millimeters, volume is 3-200 microlitres.
5. self-priming Dry-type biochemical detection means as claimed in claim 3, it is characterised in that the flow-guiding channel, which is selected, to be included
The material of hydrophilic substance is made, or the flow-guiding channel is transparent material of the surface Jing Guo hydrophilic treated.
6. self-priming Dry-type biochemical detection means as claimed in claim 1, it is characterised in that the test paper includes top
Coating and bottom an at least reaction block, each reaction block is separate and has predetermined interval；
The coating is contacted with the sample outlet hole, and the position of each reaction block is just to each detection hole.
7. self-priming Dry-type biochemical detection means as claimed in claim 1, it is characterised in that sample holes, sample outlet hole and water conservancy diversion
Being shaped as the section of passage is circular, polygon or elliptical shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710388858.3A CN107064484A (en) | 2017-05-25 | 2017-05-25 | Self-priming Dry-type biochemical detection means |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710388858.3A CN107064484A (en) | 2017-05-25 | 2017-05-25 | Self-priming Dry-type biochemical detection means |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107064484A true CN107064484A (en) | 2017-08-18 |
Family
ID=59610790
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710388858.3A Pending CN107064484A (en) | 2017-05-25 | 2017-05-25 | Self-priming Dry-type biochemical detection means |
Country Status (1)
| Country | Link |
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| CN (1) | CN107064484A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1225450A (en) * | 1997-12-24 | 1999-08-11 | 泰尔茂株式会社 | Test paper and analyte measuming tip |
| CN1837823A (en) * | 2005-03-21 | 2006-09-27 | 邓雪梅 | Animal epidemic disease detection kit and detection method thereof |
| US7494818B1 (en) * | 2001-12-28 | 2009-02-24 | Polymer Technology Systems, Inc. | Method for determining concentration of multiple analytes in a single fluid sample |
| CN102680672A (en) * | 2012-05-08 | 2012-09-19 | 艾康生物技术(杭州)有限公司 | Detection device |
| CN106198950A (en) * | 2016-07-08 | 2016-12-07 | 艾康生物技术(杭州)有限公司 | For depositing paper box and the sample testing apparatus of Test paper |
| CN106680476A (en) * | 2017-01-22 | 2017-05-17 | 英科新创(厦门)科技有限公司 | Immune-layer detection device for secretion samples |
| CN106680490A (en) * | 2016-12-03 | 2017-05-17 | 百奥森（江苏）食品安全科技有限公司 | Position limiting type immunofluorescence chromatography detection card |
| CN206788183U (en) * | 2017-05-25 | 2017-12-22 | 杭州博旭生物技术有限公司 | Self-priming Dry-type biochemical detection means |
-
2017
- 2017-05-25 CN CN201710388858.3A patent/CN107064484A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1225450A (en) * | 1997-12-24 | 1999-08-11 | 泰尔茂株式会社 | Test paper and analyte measuming tip |
| US7494818B1 (en) * | 2001-12-28 | 2009-02-24 | Polymer Technology Systems, Inc. | Method for determining concentration of multiple analytes in a single fluid sample |
| CN1837823A (en) * | 2005-03-21 | 2006-09-27 | 邓雪梅 | Animal epidemic disease detection kit and detection method thereof |
| CN102680672A (en) * | 2012-05-08 | 2012-09-19 | 艾康生物技术(杭州)有限公司 | Detection device |
| CN106198950A (en) * | 2016-07-08 | 2016-12-07 | 艾康生物技术(杭州)有限公司 | For depositing paper box and the sample testing apparatus of Test paper |
| CN106680490A (en) * | 2016-12-03 | 2017-05-17 | 百奥森（江苏）食品安全科技有限公司 | Position limiting type immunofluorescence chromatography detection card |
| CN106680476A (en) * | 2017-01-22 | 2017-05-17 | 英科新创(厦门)科技有限公司 | Immune-layer detection device for secretion samples |
| CN206788183U (en) * | 2017-05-25 | 2017-12-22 | 杭州博旭生物技术有限公司 | Self-priming Dry-type biochemical detection means |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964505A (en) * | 2017-12-28 | 2018-04-27 | 广州市宝创生物技术有限公司 | Multi-functional nucleic acid detection apparatus |
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Application publication date: 20170818 |