CN107050145A - Application of the Black Box Tracing fruit extract in treatment antiaging agent is prepared - Google Patents

Abstract

The present invention relates to application of the Black Box Tracing fruit extract in antiaging agent is prepared.Black Box Tracing fruit extract can be prepared into the medicine or health products for being adapted to oral any formulation with the preparation method of drug regimen, routinely preparation alone or that have with other anti-aging effects.Black Box Tracing fruit extract has very strong anti-aging ability.

Description

Application of Aronia nigra fruit extract in the preparation of anti-aging medicine

technical field

The invention belongs to the application field of health food and medicine, and in particular relates to the application of the fruit extract of Aronia nigricarpa in the preparation of anti-aging medicine or health care product.

Background technique

Aging is a natural and inevitable process of organisms with the passage of time. It is a complex natural phenomenon, manifested as structural degeneration, functional decline, and decreased adaptability and resistance. Aging has always been the core link in the life process, and it is also an important issue that affects the healthy development of the entire human society. Anti-aging drugs can delay aging to a certain extent, promote physical health, and maintain better intelligence and physical strength within the life span determined by genetic factors. At present, anti-aging drugs can be divided into two categories: traditional Chinese medicine and modern medicine. For example, drugs such as Naoyizine, Naofukang, and Sibeline can improve cerebral blood circulation and promote brain cell metabolism, but chemically synthesized drugs have high cost, high toxicity, and strong side effects, which will affect endocrine function and blood coagulation mechanism. Fatigue, irritability, increased blood pressure, edema, insomnia and other side effects. Therefore, it is of great significance to find biologically active and anti-aging substances from natural plants for use in medicine, food and health care products.

Aornia mealnocarpa Elliot, also known as Aronberry and Elliot, is a precious tree species with edible, medicinal, garden and ecological values. Native to northeastern North America, it is the main economic tree species introduced in Liaoning Province in recent years.

Contents of the invention

The present invention aims to seek the development and utilization of new and efficient anti-aging medicines or health care products, and proposes the application of Aronia fruit extracts in the preparation of anti-aging medicines or health care products.

The present invention adopts the fruit extract of Aronia nigricarpa as a raw material, which is used alone or in combination with other drugs with anti-aging effects, and is prepared into any dosage form of anti-aging drugs suitable for oral administration or in combination with other drugs with anti-aging effects. Health care products, such as oral liquids, tablets, capsules, granules, or other injection dosage forms.

What the present invention adopts is the fruit extract of Aronia nigriflora, which is induced by D-galactose to induce rapid aging of mice. It is obtained through the in vivo experiment of aging model mice that the fruit extract of Aronia nigriflora It can inhibit the decline of brain index and learning and memory ability in aging mice, as well as the decline of the content of monoamine neurotransmitters norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in brain tissue, and maintain Integrity and good shape of hippocampus tissue structure in aging mice; inhibition of DNA damage during aging. Therefore, the fruit extract of Aronia nigra has anti-aging effects, and it is a good choice for developing new and efficient natural anti-aging drugs or health products.

Description of drawings

Figure 1 shows the comparison results of the brain coefficients of mice in each group.

Figure 2 is a comparison of the levels of monoamine neurotransmitters (norepinephrine, dopamine, 5-hydroxytryptamine) in mice in each group.

Figure 3 is the HE staining of the hippocampal tissues of mice in each group.

Fig. 4 is a comparison chart of DNA damage in brain tissue cells of mice in each group.

Fig. 5 is a statistical diagram of DNA damage data in brain tissue cells of mice in each group.

detailed description

Example 1 Application of Aronia nigra fruit extract in the preparation of anti-aging drugs

(1) Preparation method

Preparation of Aronia fruit extract: Weigh 100 g of Aronia fruit extract, soak it in 60% ethanol at 60-70°C for 1 hour, put it into an ultrasonic cleaner for 30 minutes, and do so twice in a row. After the macroporous adsorption resin AB-8 is purified, the extract is concentrated by a rotary evaporator, and vacuum freeze-dried to obtain a dry powder of the Aronia fruit extract.

After testing, the dry powder of the extract mainly contains 47.6% of cyanidin 3-arabinoside and 46.4% of cyanidin 3-galactoside.

(2) drug efficacy test

Animal feeding and handling: The experimental animals were fed for 1 week to adapt to the conditions of the laboratory, kept in separate cages, sterilized indoors, and the litter was changed every two days. The control laboratory light is 12h bright-12h dark, the indoor temperature is 20±1°C, and the humidity is 55±10%.

Grouping of experimental animals: 60 male mice, weighing 30±2g, were randomly divided into 4 groups as follows, 15 in each group.

(1) normal control group (N);

(2) aging model group (M);

(3) Administration group: Rowan fruit extract group 15mg/kg (daily) (L);

(4) Administration group: Rowan fruit extract group 30mg/kg (daily) (H)

In the normal control group, 15 mice were subcutaneously injected with normal saline, and the rest were injected with D-galactose (150 mg/kg), once a day for 8 weeks. Administration was administered while the model was being established. In addition to the normal control group and the aging model group mice were given normal saline drinking water, the mice in the administration group were given 15 mg/kg and 30 mg/kg of Sorbus fruit extract by intragastric administration, and weighed weekly. And record the body weight of the mice. At the 8th week of the experiment, the test was carried out.

1. Water maze test: Table 1 shows the comparison results of the incubation period and the number of crossing times in the water maze test for mice in each group.

Table 1 Comparison of the latency and crossing times of mice in each group in the water maze test

^a p<0.01, vs. Control; ^b p<0.01, vs. Model (after eight weeks of administration).

It can be seen from Table 1 that the water maze experiment found that the platform escape latency time of the mice in the aging model group was significantly higher than that of the normal group, and the administration of the Aronia fruit extract could significantly reduce the platform escape latency (vs. group p<0.01); the number of platform crossings in the model group was significantly lower than that in the normal control group (p<0.01), and the administration group could significantly increase the number of platform crossings (vs. model group p<0.01), and the administration group H group Significantly higher than the normal control group (p<0.01). The experimental results show that the fruit extract of Aronia nigra can significantly improve the decline of learning and memory ability in the aging process of mice, and it is dose-related.

2. The mice were sacrificed after blood collection, the brain tissue was collected and weighed, and the brain coefficient was calculated. The comparison results of the brain coefficients are shown in Figure 1.

As shown in Figure 1, the brain coefficient of mice in the aging model group was significantly lower than that of the normal group (8.98vs 10.96, p<0.01), and the administration of Aronia nigricarpa fruit extract could interfere with the decrease of brain coefficient caused by aging, compared with In the aging model group, administration group H can significantly inhibit the reduction of the brain coefficient of mice (10.70, p<0.01). The experimental results show that Aronia fruit extract has a significant intervention effect on brain atrophy in the aging process of mice in a dose-dependent manner.

3. Detect the content of monoamine neurotransmitters norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in the brain tissue, the results are shown in Figure 2.

As shown in Figure 2, the contents of noradrenaline, dopamine, and 5-hydroxytryptamine in the mice in the aging model group were significantly lower than those in the normal group, and the administration of the fruit extract of Aronia nigricarpa can increase the levels of three levels induced by D-galactose. The content of a monoamine neurotransmitter decreased, and it significantly increased the content of dopamine (p<0.05 in the administration group L, p<0.001 in the administration group H), but the contents of noradrenaline and serotonin There was no significant change. The experimental results show that Aronia fruit extract has a certain intervention effect on the decline of emotional memory in the aging process of mice, and it is dose-related.

4. HE staining was used to observe the structure and morphology of the hippocampal tissue of aging mice, and the results are shown in Figure 3.

As shown in Figure 3, the number of hippocampal tissue cells in the aging model group mice was significantly less than that in the normal control group mice, and the arrangement was sparse and the structure was irregular. The administration of Aronia nigra fruit extract can inhibit the reduction of the number of hippocampal cells in aging mice, maintain the stability of cell morphology and structure, and inhibit the occurrence of degeneration. The experimental results show that the fruit extract of Aronia nigra can maintain the integrity and good shape of the hippocampal tissue structure of aging mice.

5. The comet test was used to detect DNA damage in brain tissue, and CASP analysis software was used to calculate (Tail Length, Tail DNA content (Tail DNA%), Tail Moment, Olive Tail Moment (OTM), etc. Related parameters. The results are shown in Figure 4 and Figure 5.

As shown in Figure 4 and Figure 5, compared with the normal group, the DNA of brain cells in the aging model group has obvious tailing phenomenon, and there are significant differences in the length of the tail, the amount of DNA in the tail, the tail and the Olive tail. (p<0.001). The administration of Aronia nigra fruit extract can inhibit the accumulation of DNA damage in a dose-related manner, with significant differences (p<0.001). The experimental results showed that the fruit extract of Aronia adenocarpus had a significant inhibitory effect on cellular DNA damage.

The results showed that the fruit extract of Aronia nigra can inhibit the decline of brain index and learning and memory ability in aging mice, as well as the monoamine neurotransmitters norepinephrine (NE), dopamine (DA), and serotonin in brain tissue. The decline of (5-HT) content, and maintain the integrity and good shape of the hippocampus tissue structure of aging mice; inhibit DNA damage in the aging process. It shows that it has anti-aging effect, and it is dose-related. Conclusion: Aronia fruit extract can reduce age-related changes, improve the quality of life in old age, and has strong anti-aging ability. It is a good choice for the development of new and efficient natural anti-aging drugs or health products.

Claims (4)

1. Black Box Tracing fruit extract is alone or has the drug regimen of anti-aging effects anti-in preparation treatment with other Application in senescence drug.

2. application as claimed in claim 1, it is characterised in that：Described Black Box Tracing fruit extract is alcohol extracting chemical combination Thing.

3. application as claimed in claim 1 or 2, it is characterised in that：The preparation of described Black Box Tracing fruit extract： Black Box Tracing fruit 100g is weighed, 1h is soaked at 60-70 DEG C with 60% ethanol, is put into ultrasonic cleaning machine ultrasonic 30min, continuous 2 times, then uses Rotary Evaporators concentrated extracting solution, by vacuum refrigeration through macroporous absorbent resin AB-8 after purification Dry Black Box Tracing fruit extract dry powder.

4. application as claimed in claim 1 or 2, it is characterised in that：The dosage of described Black Box Tracing fruit extract For 0.3-3mg/d.