CN107048373A - A kind of ultra-fine mulberry leaf powder and preparation method thereof - Google Patents
A kind of ultra-fine mulberry leaf powder and preparation method thereof Download PDFInfo
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The invention discloses a kind of ultra-fine mulberry leaf powder and preparation method thereof, comprise the following steps：（1）Removal of impurities mulberry leaf are added in pure water, broken wall treatment is then carried out；Then mulberry leaf solution is obtained by ultrasonically treated again；（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；（4）Treatment fluid concentration is prepared into concentrate；（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder.Ash content, mercury, arsenic, the indices of total plate count content for the final product that the present invention is obtained lower significantly, and with higher nutritional ingredient and abundant bioactive substance, achieve unexpected effect, be more suitable for health-care material.
Description
Technical field
The present invention relates to a kind of mulberry leaf powder, and in particular to a kind of ultra-fine mulberry leaf powder and preparation method thereof.
Background technology
Mulberry leaf can not only be to breed silkworms, but also has high nutrition and medical value, mulberry leaf quilt in motherland's medical science
It is classified as Chinese medicine, the work that will be handed on from age to age of Ancient Times in China pharmacy man Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Described in " mulberry leaf can count pan-fried Dai Ming, and informal dress can
Make us clever, colour of skin gloss bleaches, light year is old, beauty treatment stomach invigorating, spirit sedation, referred to as mulberry leaf ".
At present, it is first to dry mulberry leaf using electrical heating, drying temperature generally exists to the main technique of mulberry leaf processing and utilization
More than 100 DEG C, then crushed with mechanical crusher, mulberry leaf powder particles are obtained, after high-temperature sterilization, are added to food or feed
In.However, high temperature electrical drying can be destroyed the original nutritional ingredient of mulberry leaf and chlorophyll with sterilizing, the mulberry of acquisition
Leaf powder particles color and luster is changed into yellow green from green；It is 40~80 mesh with the mulberry leaf powder particle diameter of mechanical crushing, due to mulberry leaf powder
Particle diameter is larger, and its nutritional ingredient and bioactive substance can not be fully absorbed by human body is most effective；Use common process simultaneously
Often lead, arsenic, mercury, objectionable impurities content exceed food additives License Value in production mulberry leaf powder, and not reaching food additives will
Ask.Because mulberry leaf have abundant trophism and several functions composition, can as functional food desirable material, with
Scientific and technological economic development, the consumption quality and the level of consumption of people will be improved constantly, and health care consciousness also progressively strengthens, health mulberry leaf system
Product increasingly will be paid close attention to by people, but existing preparation method can not obtain particle diameter suitable for absorption of human body and nutritional ingredient
Good mulberry leaf powder.
As people are constantly studied the chemical composition of mulberry leaf, pharmacological action, clinical practice, mulberry leaf are a kind of trophic structures
Unusual abundant natural materials, include multivitamin, trace element, amino acid, crude protein, string, flavonoids and many
Phenols component, also containing a variety of bioactive substances beneficial to human body, with hypoglycemic, lowering blood pressure and blood fat, fat-reducing, resists
The functions such as tumour, anti-aging, removing toxic substances.Therefore, the new method for preparing mulberry leaf powder is developed, should try one's best reservation mulberry in process
The original bioactivity of leaf, especially obtains nanoscale mulberry leaf powder further to improve the quality and bioactivity of mulberry leaf, by it
Applied to biomaterial, with positive meaning.
The content of the invention
It is an object of the present invention to provide a kind of ultra-fine mulberry leaf powder and preparation method thereof, to obtain the nanoscale mulberry leaf of high-quality
Powder, specific surface area is big, so as to give full play to the high nutrition composition and abundant bioactive substance and function of mulberry leaf powder, and makes
Its health care compositio is obtained to be easier to be absorbed by the body.
To reach above-mentioned purpose, the technical solution adopted by the present invention is：A kind of preparation method of ultra-fine mulberry leaf powder, including such as
Lower step：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min.
In above-mentioned technical proposal, step（1）In, removal of impurities mulberry leaf add pure water immersion at least 30min, and bath raio is 30~50
∶1。
In above-mentioned technical proposal, step（1）In, the broken wall treatment is broken under 65000~70000rpm rotating speed
Wall processing, the time is 45~50 minutes；The ultrasonically treated power is 1000~1200W, 30~50KHz of frequency, and the time is
10~15 minutes.The present invention, using ultrasonically treated, is not traditional raising dispersiveness, but further broken particles, improves broken
The crushing effect of wall processing, so as to improve removal of impurity and improve enzymolysis processing effect.
In above-mentioned technical proposal, step（2）In, the cellulase is AE202 cellulases.Cellulase of the present invention is used
Amount is considerably less, maintains the nutritive value and bioactivity of mulberry leaf.
In above-mentioned technical proposal, step（2）In, the addition of the olive oil for mulberry leaf liquor capacity 0.6%~
0.8%；The invention adds olive oil in enzymolysis, on the basis of cellulase consumption is reduced, with reference to pre-treatment with
And micronization, using olive oil, excellent hydrolysis result can be obtained, so as to obtain particle diameter suitable for absorption of human body and nutrition
The mulberry leaf powder that composition is good, bioactivity is abundant.
In above-mentioned technical proposal, step（3）In, the filtration washing number of times is 2~5 times.The invention broken
Mulberry leaf are digested after wall ultrasound, then heated using microwave, the hazardous substance of natural mulberry leaf residual can be effectively removed, pass through microwave
Heating causes enzyme inactivation, is not dried, then by multiple times of filtration washing, so as to obtain that levels of toxic substances is extremely low, nutrition
Stirring ball-milling processing forerunner's raw material that component content is high, bioactivity is abundant, keeps the original high nutrition composition of mulberry leaf and abundant
Bioactive substance.
In above-mentioned technical proposal, step（4）In, the solid content of the concentrate is 50~70%；That is solids in concentrate
Mass fraction be 50~70%.Excellent raw material form is provided to be finally stirred ball-milling treatment, optimal processing effect is reached
Really, so as to realize the preparation of nanoscale mulberry leaf powder.
In above-mentioned technical proposal, step（6）In, the rotating speed of preferably described stirring ball-milling processing is 30000~35000rpm；
The flow of concentrate is 140~160mL/min.
In above-mentioned technical proposal, step（6）In, the temperature of the freeze-drying is -30~-20 DEG C, and pressure is 3~5Pa,
Evaporate 2~5mm of liquid layer thickness.
The invention also discloses the ultra-fine mulberry leaf powder obtained according to above-mentioned preparation method.
The present invention utilizes stirring ball-milling revolving bed first, under stirring ball-milling effect so that mulberry leaf powder nucleation, forms nanometer
Level product, the problems such as solving that prior art mulberry leaf powder particle diameter is excessive, be unfavorable for absorption of human body.According to the physico of powder material
Learn the depth of property and pulverizing processing technology, it is considered that can not obtain particle diameter with the method for mechanical crushing such as ball milling and be less than
0.1 micron of product, let alone nanometer product；If preparing nanometer product using special Preparation equipment, yield with
And yield is very low, and preparation is complicated, is unfavorable for industrialization.Present invention broken wall treatment first, followed by ultrasonically treated,
In conjunction with olive oil enzymolysis, the concentrate for limiting solid content is then prepared, is finally handled, is received so as to prepare using agitating ball mill
Meter level mulberry leaf powder, especially with reference to whole technique, nanometer product is prepared using micro mist processing equipment, is achieved unexpected
Technique effect.
The present invention prepares nanoscale mulberry leaf powder first, and particle diameter is 34 nanometers, and clean odorless, especially with excellent
Different nutritive value, amino acid composition loses few compared with untreated；It is demonstrated experimentally that the ash for the final product that the present invention is obtained
Point, the indices of lead, mercury, arsenic, total plate count and E. CoIi content lower significantly, especially with healthcare function into
Point content is obviously improved with the medicinal function factor compared with prior art, is achieved unexpected effect, is more suitable for biomaterial.
The invention also discloses a kind of preparation method of ultra-fine mulberry leaf powder spansule, comprise the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）Ultra-fine mulberry leaf powder and spansule shell are assembled, ultra-fine mulberry leaf powder spansule is obtained.
The invention also discloses a kind of ultra-fine mulberry leaf powder spansule, its preparation method comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）Ultra-fine mulberry leaf powder and spansule shell are assembled, ultra-fine mulberry leaf powder spansule is obtained.
The invention also discloses a kind of ultra-fine mulberry leaf powder beverage, its preparation method comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and xylitol compatibility, ultra-fine mulberry leaf powder beverage is obtained.
The invention also discloses a kind of ultra-fine mulberry leaf nutrient powder, its preparation method comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and black sesame powder compatibility, ultra-fine mulberry leaf nutrient powder is obtained.
Because above-mentioned technical proposal is used, the present invention has following advantages compared with prior art：
1. the present invention is from mulberry leaf, first finally using being dried after stirring ball-milling processing by broken wall, ultrasound, enzymolysis, concentration
Secondary to prepare nanoscale mulberry leaf powder, particle diameter is 34 nanometers, and specific surface area is big, can give full play to the activity and function of mulberry leaf powder,
It especially as biomaterial for medical purpose, more can really be absorbed by the body, achieve unexpected technique effect.
2. the functional character of nanoscale mulberry leaf powder disclosed by the invention is substantially, particularly amino acid composition is compared with untreated
Change is small, and mulberry leaf powder active function is excellent, and the especially component content with healthcare function and the medicinal function factor is more existing
There is technology to be obviously improved.
3. the present invention utilizes enzymolysis processing mulberry leaf, especially with AE202 cellulases, handled in qualifications,
Mulberry leaf nutrient value, the loss of bioactive substance are avoided while mulberry leaf are refined；Extra acid or alkali is not added, therefore
Extra side effect will not be produced, high degree keeps the self-value of mulberry leaf, especially drops blood with special healthcare function
The DNJ of sugar, can be high by the gamma-amino acid content of blood pressure norcholesterol reducing blood lipid.
4. mulberry leaf are creatively carried out broken wall treatment by the present invention, then by ultrasonically treated, obtain mulberry leaf solution；Realization pair
The broken wall of mulberry leaf cell membrane, in the case where ensureing that mulberry leaf powder active ingredient is not destroyed, beneficial to the cracking of mulberry leaf powder, more causes
Mulberry leaf whitewash is refined, and is that follow-up stirring ball-milling processing lays the first stone, so as to combine enzymolysis, stirring ball-milling processing, is obtained nanoscale
Edible-type, high-performance mulberry leaf powder.
5. the invention is handled mulberry leaf concentrate using stirring ball-milling technology, solid content be 50~
Under 70%, nanoscale mulberry leaf powder can be not only obtained, and avoids the loss of nutritional ingredient, the height of mulberry leaf powder is at utmost realized
Nutritional ingredient and abundant bioactive substance.
6. the present invention is moist using microwave treatment enzymolysis liquid, finally using freeze-drying, thus the ultra-fine mulberry leaf obtained
Powder has higher health function and bioactivity, it is to avoid existing drying means causes the reduction of mulberry leaf bioactivity, battalion
The problem of foster material is lost in.
Brief description of the drawings
Fig. 1 is the grain size distribution of the ultra-fine mulberry leaf powder of embodiment three；
Fig. 2 is the pictorial diagram of the ultra-fine mulberry leaf powder of embodiment three.
Embodiment
With reference to embodiment, the invention will be further described：
A kind of preparation method of the ultra-fine mulberry leaf powder of embodiment one, comprises the following steps：
（1）Mulberry leaf surface impurity is removed, removal of impurities mulberry leaf are obtained；
（2）Removal of impurities mulberry leaf are added in pure water and soak 30min, bath raio is 30: 1, then carries out broken wall treatment；Then again by super
Sonication, obtains mulberry leaf solution；The broken wall treatment carries out broken wall treatment under 65000rpm rotating speed, and the time is 45 minutes；
The ultrasonically treated power is 1000W, and frequency 30KHz, the time is 10 minutes；
（3）AE202 cellulases are added in mulberry leaf solution, adds after the olive oil of mulberry leaf liquor capacity 0.8%, enzymolysis and obtains
To enzymolysis liquid；
Enzyme dosage is 600 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 40 DEG C, and enzymolysis time is 2.5 hours, and pH value control exists
7.5；
（4）Enzymolysis liquid is subjected to filtering, water washing after microwave heating treatment, is repeated 3 times, obtains treatment fluid；The microwave heating
Temperature be 60 DEG C, the time be 0.3 hour；
（5）Treatment fluid concentration is prepared into the concentrate that solid content is 70%；
（6）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000rpm；The flow of concentrate is 300mL/min；The temperature of the freeze-drying is -20
DEG C, pressure is 3Pa, evaporation liquid layer thickness 5mm.
The particle diameter of above-mentioned ultra-fine mulberry leaf powder is 35 nanometers.
A kind of preparation method of the ultra-fine mulberry leaf powder of embodiment two, comprises the following steps：
（1）Mulberry leaf surface impurity is removed, removal of impurities mulberry leaf are obtained；
（2）Removal of impurities mulberry leaf are added in pure water and soak at least 30min, bath raio is 50: 1, then carries out broken wall treatment；Then pass through again
Cross ultrasonically treated, obtain mulberry leaf solution；The broken wall treatment carries out broken wall treatment under 70000rpm rotating speed, and the time is 50 points
Clock；The ultrasonically treated power is 1200W, and frequency 50KHz, the time is 15 minutes；
（3）AE202 cellulases are added in mulberry leaf solution, adds after the olive oil of mulberry leaf liquor capacity 0.6%, enzymolysis and obtains
To enzymolysis liquid；
Enzyme dosage is 800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 40 DEG C, and enzymolysis time is 2 hours, and pH value is controlled 6.5；
（4）Enzymolysis liquid is subjected to filtering, water washing after microwave heating treatment, is repeated 2 times, obtains treatment fluid；The microwave heating
Temperature be 70 DEG C, the time be 0.6 hour；
（5）Treatment fluid concentration is prepared into the concentrate that solid content is 50%；
（6）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3800rpm；The flow of concentrate is 400mL/min；The temperature of the freeze-drying is -30
DEG C, pressure is 5Pa, evaporation liquid layer thickness 2mm.
The particle diameter of above-mentioned ultra-fine mulberry leaf powder is 35 nanometers.
A kind of preparation method of the ultra-fine mulberry leaf powder of embodiment three, comprises the following steps：
（1）Mulberry leaf surface impurity is removed, removal of impurities mulberry leaf are obtained；
（2）Removal of impurities mulberry leaf are added in pure water and soak at least 30min, bath raio is 50: 1, then carries out broken wall treatment；Then pass through again
Cross ultrasonically treated, obtain mulberry leaf solution；The broken wall treatment carries out broken wall treatment under 68000rpm rotating speed, and the time is 45 points
Clock；The ultrasonically treated power is 1100W, and frequency 40KHz, the time is 15 minutes；
（3）AE202 cellulases are added in mulberry leaf solution, adds after the olive oil of mulberry leaf liquor capacity 0.7%, enzymolysis and obtains
To enzymolysis liquid；
Enzyme dosage is 700 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 40 DEG C, and enzymolysis time is 2.5 hours, and pH value control exists
6.5；
（4）Enzymolysis liquid is subjected to filtering, water washing after microwave heating treatment, is repeated 5 times, obtains treatment fluid；The microwave heating
Temperature be 70 DEG C, the time be 0.5 hour；
（5）Treatment fluid concentration is prepared into the concentrate that solid content is 60%；
（6）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3500rpm；The flow of concentrate is 350mL/min；The temperature of the freeze-drying is -30
DEG C, pressure is 3Pa, evaporation liquid layer thickness 5mm.
Fig. 1 is the grain size distribution of the ultra-fine mulberry leaf powder of above-described embodiment three, it can be seen that mulberry leaf powder prepared by the present invention
Footpath reaches nanoscale first, is 34 nanometers.
The ultra-fine mulberry leaf powder that the present invention is obtained contains 17 kinds of amino acid, wherein the content of 8 kinds of amino acid needed by human is shown in
Table 1；2 are shown in Table with special healthcare function component content ratio；Index of correlation testing result is shown in Table 3., it is apparent that this hair
The mulberry leaf powder of bright preparation not only achieves particle diameter and is less than 50 nanometers of unexpected technique effect, and amino acid content,
Existing product is also surmounted significantly in terms of health care compositio；Therefore the ultra-fine mulberry leaf powder that prepared by the present invention is maximally maintained mulberry leaf
Original high nutrition composition and abundant bioactive substance, particularly nanoscale can really realize human body efficient absorption, be
Really application provides basis to mulberry leaf powder, can make capsule especially spansule, direct mouth with the assembling of existing spansule shell
Diabetes are had notable auxiliaring effect by clothes；It is able to can also will such as surpass according to 100: 8 mass ratio with other materials compatibility
Thin mulberry leaf powder and xylitol compatibility, obtain ultra-fine mulberry leaf powder beverage, can also be according to 2: 1 mass ratio by ultra-fine mulberry leaf powder and sesame
Numb powder compatibility, obtains ultra-fine mulberry leaf nutrient powder.
Claims (10)
1. a kind of preparation method of ultra-fine mulberry leaf powder, it is characterised in that comprise the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min.
2. the preparation method of ultra-fine mulberry leaf powder according to claim 1, it is characterised in that：Step（1）In, removal of impurities mulberry leaf are added
Pure water soaks at least 30min, and bath raio is 30~50: 1；The broken wall treatment is carried out under 65000~70000rpm rotating speed
Broken wall treatment, the time is 45~50 minutes；The ultrasonically treated power is 1000~1200W, 30~50KHz of frequency, time
For 10~15 minutes.
3. the preparation method of ultra-fine mulberry leaf powder according to claim 1, it is characterised in that：Step（2）In, the cellulase
For AE202 cellulases；The addition of the olive oil is the 0.6%~0.8% of mulberry leaf liquor capacity；Step（4）In, it is described dense
The solid content of contracting thing is 50~70%；Step（6）In, the rotating speed of the stirring ball-milling processing is 3400~3500rpm；Concentrate
Flow be 340~350mL/min；The temperature of the freeze-drying is -30~-20 DEG C, and pressure is 3~5Pa, and evaporation liquid layer is thick
Spend 2~5mm.
4. the ultra-fine mulberry leaf powder that according to claim 1 prepared by the preparation method of ultra-fine mulberry leaf powder.
5. a kind of preparation method of ultra-fine mulberry leaf powder spansule, it is characterised in that comprise the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）Ultra-fine mulberry leaf powder and spansule shell are assembled, ultra-fine mulberry leaf powder spansule is obtained.
6. a kind of ultra-fine mulberry leaf powder spansule, it is characterised in that the preparation method of the ultra-fine mulberry leaf powder spansule includes
Following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）Ultra-fine mulberry leaf powder and spansule shell are assembled, ultra-fine mulberry leaf powder spansule is obtained.
7. a kind of preparation method of ultra-fine mulberry leaf powder beverage, it is characterised in that comprise the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and xylitol compatibility, ultra-fine mulberry leaf powder beverage is obtained.
8. a kind of ultra-fine mulberry leaf powder beverage, it is characterised in that the preparation method of the ultra-fine mulberry leaf powder beverage comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and xylitol compatibility, ultra-fine mulberry leaf powder beverage is obtained.
9. a kind of ultra-fine mulberry leaf nutrient powder, it is characterised in that the preparation method of the ultra-fine mulberry leaf nutrient powder comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and black sesame powder compatibility, ultra-fine mulberry leaf nutrient powder is obtained.
10. a kind of preparation method of ultra-fine mulberry leaf nutrient powder, it is characterised in that powder comprises the following steps：
（1）The mulberry leaf of surface removal of impurities are added in pure water, broken wall treatment is then carried out；Then mulberry is obtained by ultrasonically treated again
Leaf solution；
（2）Cellulase is added in mulberry leaf solution, enzymolysis liquid is obtained after adding olive oil, enzymolysis；
Enzyme dosage is 600~800 unit of activity/g mulberry leaf powders, and hydrolysis temperature is 35~40 DEG C, and enzymolysis time is 2~2.5 hours,
PH value is controlled 6.5~7.5；
（3）Enzymolysis liquid is subjected to filtration washing after microwave heating treatment, treatment fluid is obtained；The temperature of microwave heating for 60~
70 DEG C, the time is 0.3~0.6 hour；
（4）Treatment fluid concentration is prepared into concentrate；
（5）Concentrate is stirred ball-milling treatment；Then it is freeze-dried, then carries out irradiation sterilization, obtains ultra-fine mulberry leaf powder；Institute
The rotating speed for stating stirring ball-milling processing is 3000~3800rpm；The flow of concentrate is 300~400mL/min；
（6）By ultra-fine mulberry leaf powder and black sesame powder compatibility, ultra-fine mulberry leaf nutrient powder is obtained.
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