CN107047268B - A high-throughput root cultivation and automatic growth imaging system in a dark environment - Google Patents

Abstract

The invention relates to a high-throughput root cultivation and automatic growth imaging system in a dark environment. The system includes: a main frame, a root box for cultivating plant roots, and a root box that is installed on the main frame and can move relative to the main frame. The box frame is an H-shaped frame that is installed on the main frame and can move relative to the main frame, and an imaging subsystem that is installed on the H-shaped frame and can move relative to the H-shaped frame, and is installed on the main frame and connected to the root The irrigation subsystem connected with the boxes, the return water subsystem installed on the main frame and connected with the irrigation subsystem, and the shading subsystem used to shade the whole system to achieve a dark environment; wherein, multiple root boxes can be It is disassembled and installed on the root box frame; the imaging subsystem is used to image the root system in the root box; the irrigation subsystem is used to provide nutrient solution for the plants in the root box to realize soilless cultivation; the return water subsystem is used to recycle the The excess nutrient solution that seeps out of the root box is recycled.

Description

A high-throughput root cultivation and automatic growth imaging system in a dark environment

technical field

The invention relates to a root system phenotype measurement platform in a non-soil medium, in particular to a root system high-throughput cultivation and automatic growth imaging system in a dark environment.

Background technique

Plant phenotypes are key to understanding plant genotype-environment interactions. In recent years, with the improvement of efficiency and cost reduction of gene sequencing technology, the genome data of plants has been continuously increased and improved; Destructive assay. Therefore, the systematic collection of plant phenotyping information has gradually become a limiting factor in genetic breeding research and basic plant science research, and many academic institutions and companies are committed to the development of plant phenotyping platforms. At present, most of the research and development of plant phenotyping techniques focus on the aboveground parts of plants. This is mainly due to the opacity of the root growth medium and the complexity of the root system, which makes it very difficult to observe and measure the root system. The root system is an important organ for plants to absorb water and nutrient resources and respond to changes in the soil environment. Breeding new varieties by improving root system traits can effectively improve crop yield and nutrient resource utilization efficiency. Therefore, the non-destructive determination of root phenotypes in indoor cultivation and the in-depth exploration of the gene-environment-phenotype interaction mechanism of crops play a vital role in crop breeding research, plant physiology research and model parameterization.

At present, the non-destructive determination methods of root system in indoor cultivation include nuclear magnetic resonance imaging, neutron tomography and micro X-ray method. The micro-X-ray method is the most accurate non-destructive measurement method, but it can only measure the root system at the seedling stage in a soil column with a small diameter. The equipment is expensive and requires a large amount of data storage space and powerful computer cluster operations. The application of quantitative root phenotyping is limited. The root box photography method (GROWSCREEN-Rhizo, 2012) can cultivate plants with high throughput, irrigate precisely, and automatically image the root system in the narrow root box, but it cannot obtain the complete root system structure, and the equipment cost is high and the space utilization rate is high. Low. Obtaining root phenotypes in non-soil media is another important way to conduct root research. Rhizotubes (2015) based on the LemnaTec system can obtain images of the root system close to the surface of the circular tube in large quantities, but as the throughput increases, the distance of plant transmission also increases, long-distance transportation and frequent start and stop Affects plant growth (lodging). Researchers such as Adu (2014) used a plane scanner as the main body of imaging and cultivation, and pasted the germination paper on the transparent panel of the scanner. The root system grew in it, and the bottom of the germination paper extended into the nutrient solution to absorb nutrients. In this method, the germination paper is simultaneously used as a growth medium, a nutrient absorption medium and a light-shielding material. However, as the throughput increases, the number of scanners also increases proportionally, and limited by the water absorption capacity of the germination paper, this method cannot cultivate larger plants. In summary, the existing root phenotyping platforms cannot achieve a good balance between high-throughput, fully automated, real-time determination, precise growth environment control, and low cost. Therefore, there is an urgent need to design and develop a high-throughput, automated, real-time root phenotype rapid assay platform to meet the requirements of large-scale scientific research and deeply explore the mechanism of crop gene-environment-phenotype interaction.

Contents of the invention

In view of the above problems, the object of the present invention is to provide a high-throughput root cultivation and automatic growth imaging system in a dark environment, which can carry multiple sets of root box racks, and a single root box rack can carry multiple root boxes. The three-dimensional movement and rotation of the system and the sliding of the root box frame, integrated irrigation, water return and shading subsystems, realize real-time imaging of root growth in a dark environment, and can be used in high-throughput, fully automated, real-time determination and high space A good balance between utilization rate and low cost can meet the requirements of large-scale scientific research and provide a complete solution for analyzing various root phenotypic parameters.

In order to achieve the above object, the present invention adopts the following technical solutions: a high-throughput root cultivation and automatic growth imaging system in a dark environment, characterized in that the system includes: a main frame, a root box for cultivating plant roots, and an installation The root box frame that is on the main frame and can move relative to the main frame, the H-shaped frame that is installed on the main frame and can move relative to the main frame, is installed on the H-shaped frame The imaging subsystem that can move relative to the H-shaped frame, the irrigation subsystem that is installed on the main frame and communicates with the root box, is installed on the main frame and communicates with the irrigation sub-system The return water subsystem connected with the system, and the light-shielding subsystem used to shield the entire system to achieve a dark environment; wherein, a plurality of the root boxes are detachably installed on the root box frame; the imaging sub-system The system is used to image the root system in the root box; the irrigation subsystem is used to provide nutrient solution for the plants in the root box to realize soilless cultivation; the return water subsystem is used to recycle the The excess nutrient solution seeping out of the box is recycled.

The main frame includes a plurality of upper beams and lower beams, and the upper beams and the lower beams are connected by vertical beams; the top and inner sides of the two parallel and opposite upper beams are respectively fixed with a top linear guide rail and an inner linear guide rail, Bottom linear guides and bottom ball screws parallel to the two inner linear guides are arranged on the inner sides of the two parallel and opposite lower beams, and the top linear guides, inner linear guides and bottom linear guides are respectively slidably provided with Top slider, inner slider and bottom slider; the root box rack is installed on the main frame through the top slider and can move relative to the main frame; the H-shaped frame passes through the inner The slider and the bottom slider are mounted on the main frame and can move relative to the main frame.

The root box includes a culture body arranged in the center, a background cloth covering the front and rear sides of the culture body, spacers respectively arranged on the left and right sides and the bottom edge of the culture body, and arranged on the outside of the background cloth. And the transparent splint fixedly connected with the spacer and the root box irrigation pipe arranged on the top of the culture body; the thickness of the culture body is consistent with the thickness of the spacer bar; the spacer bar at the bottom of the culture body There is a certain distance from the bottom of the culture body and the spacers on both sides to form a drainage groove at the bottom of the root box; the irrigation pipe of the root box is connected with the irrigation subsystem.

The root box frame includes two top beams arranged at intervals in parallel, a bottom beam arranged parallel to the top beams and directly below the middle interval between the two top beams, and a vertical vertical beam connecting the top beams and the bottom beams as a whole. Beams, which enclose the loading space of the root box between the top beams, bottom beams and vertical beams; the length of the top beams is greater than or equal to the width of the main frame, and the two ends of the top beams are respectively It is fixedly connected with the top slider of the main frame, so that the root box holder is installed on the main frame; the length of the bottom beam is smaller than the width of the main frame.

The H-shaped frame includes vertical slides on both sides, horizontal beam slides that are slidably connected to the two vertical slides at both ends, and bases that are fixedly connected to the bottoms of the two vertical slides and are slidably arranged on The rotary table on the horizontal beam slide, the two vertical slides and the horizontal beam slide form an H shape, and the imaging subsystem is fixedly installed on the rotary table; the two ends of the base are respectively connected to The two bottom sliders of the main frame are fixedly connected, and the top sides of the two vertical slides are respectively fixedly connected with the two inner sliders of the main frame, so that the H-shaped rack can be relatively The main frame slides; the horizontal beam slide table can lift and slide relative to the H-shaped frame; the rotary table can slide horizontally relative to the H-shaped frame; the rotary table can rotate around its own vertical The axis of rotation rotates 360°.

The vertical slide table includes a vertical linear guide and a vertical ball screw, the vertical slider of the vertical linear guide is connected with the vertical screw nut of the vertical ball screw, and the vertical motor of the vertical ball screw drives the The vertical screw nut moves, thereby driving the vertical slider to slide relative to the vertical linear guide rail; The vertical slider is fixedly connected, and the vertical ball screw drives the movement of the vertical slider to drive the horizontal beam sliding table up and down; the horizontal beam sliding table includes a horizontal linear guide rail and a horizontal ball screw, and the horizontal linear guide rail The horizontal slider is connected to the horizontal screw nut of the horizontal ball screw, and the horizontal motor of the horizontal ball screw drives the horizontal screw nut to move, thereby driving the horizontal slider relative to the horizontal linear guide rail Sliding; the bottom screw nut of the bottom ball screw is fixedly connected with the base beam of the base, and the bottom motor of the bottom ball screw drives the bottom screw nut to move, thereby driving the H-shaped frame to slide The rotary table is fixedly connected with the horizontal slider on the horizontal linear guide rail of the horizontal beam slide, and the horizontal ball screw drives the horizontal slider to move the rotary table relative to the horizontal beam slide slide.

The imaging subsystem includes an imaging device fixedly arranged on the rotating table, a flash lamp fixedly installed under the horizontal beam sliding table with the lamp head facing down, and a reflector fixedly arranged under the lamp head of the flash lamp.

The irrigation subsystem includes a nutrient solution barrel, an irrigation self-priming pump, and a circulation self-priming pump that are connected in sequence through connecting pipes; The connecting pipes at the root box irrigation pipes are connected to each other, and the outlet ends of the root box irrigation pipes are respectively connected to the connecting pipes at one end of the nutrient solution bucket through each branch pipe; A check valve and a first solenoid valve are provided, and a second solenoid valve is also provided on the connecting pipe at the outlet end of the irrigation pipe of the root box;

The water return subsystem includes a water tank, a water tank with both ends closed and a hole at the bottom of one end, a U-shaped tank with one end closed and the other end connected to the water tank, and a water tank arranged in the water tank and connected to the irrigation subsystem. A connected submersible pump; a plurality of the water tanks are fixedly arranged on the bottom of each of the root boxes respectively, and the openings at the bottom of the water tanks face the same side; the openings at the bottom of the water tanks are connected to the water conduit, and the The bottom end of the water guide pipe is connected with the filter cloth bag; the U-shaped groove is fixedly arranged on one side of the bottom of the main frame and directly below the filter cloth bag.

The shading subsystem includes a root frame top beam interval shading module, a root frame inter-frame shading module and a main frame side shading module; wherein, the root frame top beam interval shading module is used for the top of the root frame The filling of the space between the two top beams; the light-shielding module between the root box frames is used for the light-shielding between the root box frames and between the root box frame and the main frame; the side part of the main frame is light-shielding Modules are used for shading around the main frame.

The shading module between the root boxes includes an n-type micro-shrinkage organ cover and a suspension beam. The top beam of the root box frame is fixedly connected with the upper beam of the main frame, and the sliding of the root box frame can drive the expansion and contraction of the n-type micro-shrink force organ cover; The suspension beam is fixed on the top of the main frame parallel to the retractable direction of the n-type micro-shrinkage organ cover, and the middle part of the n-type micro-shrinkage organ cover is suspended on the suspension beam; the main The side shading module of the frame adopts side shading fabrics with waterproof zippers sewn on three sides, and the side shading fabrics around the main frame are connected by the waterproof zippers to realize the shading of the main frame. Blackout all around.

Due to the adoption of the above technical scheme, the present invention has the following advantages: 1. A high-throughput root cultivation and automatic growth imaging system in a dark environment of the present invention integrates root boxes, irrigation subsystems, return water subsystems, and shading The subsystem and the real-time imaging subsystem have the functions of cultivation, measurement and monitoring, and realize real-time imaging and monitoring of the root system in a completely dark environment without human intervention, so as to facilitate the real-time reflection of the root system to various experimental treatments. 2. A high-throughput root cultivation and automatic growth imaging system in a dark environment of the present invention. The root box can be used to observe the complete root system structure. The position of the root system is fixed during the growth process, and it is loaded by cooperating with the root box frame , The transparent splint of the root box exerts uniform pressure on the root system, improving the repeatability of the experimental results. 3. A high-throughput root cultivation and automatic growth imaging system in a dark environment of the present invention integrates all motion functions by using an H-shaped frame, so that the imaging equipment of the imaging subsystem can make a straight line in the three directions of XYZ Movement and rotation, the root box frame can also slide back and forth synchronously, so as to realize the imaging of the root system on multiple root box frames, and realize the real-time determination of high-throughput automation. 4. A high-throughput root cultivation and automatic growth imaging system in a dark environment of the present invention. The shading subsystem not only solves the needs of roots growing in a dark environment, but also does not block the imaging line of sight during imaging. Each shading module Both are easy to disassemble and assemble, and are convenient for installing root boxes, transplanting seedlings and other operations. 5. In the high-throughput root cultivation and automatic growth imaging system in a dark environment of the present invention, each module can operate independently without interfering with each other. The whole system has a compact structure, takes up little space, and has strong scalability and adjustability. 6. The present invention is a high-throughput root cultivation and automatic growth imaging system in a dark environment, which can meet the requirements of large-scale scientific research and provide a complete solution for analyzing various root phenotype parameters. Science, environmental science, genotype-environment interaction and other fields have high application value.

Description of drawings

Fig. 1 is a structural representation of the present invention;

Fig. 2 is the structural representation of main framework of the present invention;

Fig. 3 is a three-dimensional exploded schematic diagram of the root box of the present invention;

Fig. 4 is a left view structural schematic diagram of the root box of the present invention;

Fig. 5 is the structural representation of root box frame of the present invention;

Fig. 6 is the structural representation of H type frame of the present invention;

Fig. 7 is the assembly schematic diagram of root box holder, H-type frame, imaging subsystem and main frame of the present invention;

Fig. 8 is a structural representation of the irrigation subsystem and the return water subsystem of the present invention;

Fig. 9 is an assembly schematic diagram of the irrigation subsystem, the return water subsystem, the root box frame, the H-shaped frame and the main frame of the present invention;

Fig. 10 is a structural schematic diagram of the shading subsystem of the present invention;

Fig. 11 is a partial structural schematic diagram of the shading subsystem of the present invention.

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

As shown in Figure 1, a kind of root system high-throughput cultivation and automatic growth imaging system provided by the present invention in a dark environment includes a main frame 1, a root box 2, a root box frame 3, an H-shaped frame 4, and an imaging sub-frame. System 5, irrigation subsystem 6, return water subsystem 7 and shading subsystem 8.

As shown in Figure 2, the main frame 1 is a 2.5m long, 2.5m wide and 1.5m high cube frame assembled by aluminum profiles, which includes four upper beams 11, four lower beams 12 and four vertical beams 13; The top linear guide rails 14 and the inner linear guide rails 15 are respectively fixed on the top and inner side of the two opposite upper beams 11; at the same time, the bottom linear guide rails parallel to the two inner linear guide rails 15 are respectively arranged on the inner sides of the two opposite lower beams 12. 16 and the bottom ball screw 17, the top linear guide 14, the inner linear guide 15 and the bottom linear guide 16 are respectively slidably provided with a top slider 141, an inner slider 151 and a bottom slider 161.

In the above-mentioned embodiment, the top linear guide rail 14 is provided with a plurality of top sliders 141, and the root box frame 3 also includes a plurality of them, so as to realize high-throughput cultivation.

As shown in Figures 3 and 4, the root box 2 includes a plate-shaped culture body 21 arranged in the center, a background cloth 22 covering the front and rear sides of the culture body 21, and hard cloths 22 respectively arranged on the left and right sides and the bottom of the culture body 21. Spacer bar 23, the outside that is arranged on the background cloth 22 of culture body 21 front and back sides and the transparent splint 24 that is fixedly connected with spacer bar 23 and the root box irrigation pipe 25 that is arranged on culture body 21 top; Wherein, the thickness of culture body 21 and The thickness of the spacer 23 is consistent, so that the transparent splint 24 can clamp the culture body 21 and the background cloth 22 in the middle, and the hard spacer 23 can support the transparent splint 24, reducing the deformation or bulging of the transparent splint 24 The spacer 23 at the bottom of the culture body 21 is a distance away from the bottom of the culture body 21 and the left and right side spacer bars 23, thereby forming a drainage groove 26 at the bottom of the root box 2, which is conducive to the infiltration of the nutrient solution to discharge the root box 2; The root box irrigation pipe 25 is provided with two rows of micropores, the diameter of which is 0.3mm, and the angle between the two rows of micropores is 150°. The culture body 21 can be a sponge or an open-pored EPDM (Ethylene Propylene Diene Monomer, ethylene propylene diene monomer) material (super soft).

In the above-mentioned embodiment, the transparent splint 24 is made of transparent polycarbonate with a thickness of 6 mm; the thickness of the culture body 21 is 20 mm; The length of the sides and bottom match.

In the above embodiment, round holes are drilled around the transparent splint 24 and at corresponding positions on the spacer bar 23 , and the transparent splint 24 and the spacer bar 23 are connected by bolts and nuts.

As shown in Fig. 5 and Fig. 7, the root box frame 3 includes a top beam 31, a bottom beam 32 and a vertical beam 33, and the two top beams 31 are arranged in parallel at intervals, and the length of the top beam 31 is greater than or equal to the width of the main frame 1. The two ends of the beam 31 are fixedly connected with the top slider 141 of the top linear guide rail 14 of the main frame 31, thereby the root box frame 3 is installed on the main frame 1, and the sliding of the root box frame 3 is realized; the bottom beam 32 and the two top Beam 31 is arranged in parallel directly below the interval in the middle of two top beams 31, and the length of bottom beam 32 is less than the width of main frame 1, facilitates root box frame 3 to move in main frame 1; In top beam 31, bottom beam 32 and vertical The loading space of the root box 2 is enclosed between the beams 33 .

In the above embodiment, the top beam 31 of the root box frame 3 is made of a 20×20mm aluminum profile with a length of 2.5m; the bottom beam 32 is made of a 40×40mm aluminum profile with a length of 2.1m.

In the above-mentioned embodiment, in order to ensure that the pressure of the transparent splint 24 on the root system at any position is consistent, a root box fixing rod (not shown in the figure) is also provided on the top beam 31 of the root box frame 3, and the root box fixing rod adopts 20×10mm Made of angled aluminum, the length is the same as the length of the top beam 31, the root box fixing rod is fixedly connected with the top beam 31 through the wide side, and fixedly connected with the transparent splint 24 of the root box 2 through the narrow side; it is arranged on both sides of the top beam 31 Two box fixing rods on the top apply pressure to the transparent splint 24 on the top of the root box 2, ensuring that the interval between the two transparent splints 24 of the root box 2 is 20mm at any position (especially at the top of the root box 2). When in use, a box fixing rod is fixedly connected with a top beam 31 first, the root box 2 is inserted into the loading space of the root box frame 3 from the interval between the two top beams 31, and the root box 2 is placed on the bottom beam 32 , and the transparent splint 24 on one side is fixedly connected with the root box fixing bar; then another box fixing bar is clamped to the top of the root box 2 and then fixedly connected with another top beam 31.

As shown in Fig. 6 and Fig. 7, the H-shaped frame 4 is 2.3m wide and 1.4m high, which includes vertical slides 41 on both sides, horizontal beam slides 42 connected to the two vertical slides 41 at both ends, Simultaneously with the base 43 that is fixedly connected with the bottom of two vertical slides 41 and the rotary table 44 that is arranged on the horizontal crossbeam slide 42, two vertical slides 41 and horizontal crossbeam slide 42 constitute H type. Wherein, the top sides of the two vertical slides 41 are respectively fixedly connected with the inner sliders 151 of the two inner linear guide rails 15 of the main frame 1, so as to ensure that the vertical slides 41 do not deflect when running and stationary; 41 includes a vertical linear guide 411 and a vertical ball screw 412, the vertical slider 413 of the vertical linear guide 411 is connected with the vertical screw nut 414 of the vertical ball screw 412, and the vertical motor 415 of the vertical ball screw 412 drives the vertical screw The nut 414 moves, thereby driving the vertical slider 415 to slide relative to the vertical linear guide rail 411; the two ends of the horizontal beam slide table 42 are respectively fixedly connected with the vertical slider 413 on the vertical linear guide rail 411 of the two vertical slide tables 41, and the vertical ball wires The rod 412 drives the vertical slider 413 through the vertical screw nut 414 to drive the horizontal beam slide 42 up and down. The horizontal beam slide 42 includes a horizontal linear guide 421 and a horizontal ball screw 422. The horizontal screw nut 424 of the ball screw 422 is connected, and the horizontal motor 425 of the horizontal ball screw 422 drives the horizontal screw nut 424 to move, thereby driving the horizontal slider 423 to slide relative to the horizontal linear guide rail 421; the two ends of the base 43 are respectively It is fixedly connected with the bottom sliders 161 of the two bottom linear guide rails 16 of the main frame 1, the bottom screw nut 171 of the bottom ball screw 17 is fixedly connected with the base beam of the base 43, and the bottom motor 172 of the bottom ball screw 17 drives the bottom screw. The rod nut 171 moves, thereby driving the H-shaped frame 4 to slide forward and backward; the rotary table 44 is fixedly connected with the horizontal slide block 423 on the horizontal linear guide rail 421 of the horizontal beam slide table 42, and the horizontal ball screw 422 drives the horizontal slide block 423 to move. The turntable 44 slides left and right with respect to the horizontal beam slide 42, and the turntable 44 can rotate 360° around its own vertical rotation axis. The top of the vertical slide table 41 is also fixedly provided with a stepper motor 416, a supporting planetary reducer 417 and a rotating arm 418, and the axis of the rotating arm 418 and the planetary reducing device 417 is fixed; When the frame 3 and the swivel arm 418 move to the vertical upward state, the H-type frame 4 and the root box frame 3 can be locked together, and the H-type frame 4 can slide forward and backward to push and pull the root box frame 3 to move synchronously; and when the swivel arm 418 When keeping the vertically downward state, the H-type frame 4 can pass freely under the root box frame 3 and the root box frame 3 remains static.

As shown in Figure 7, the imaging subsystem 5 includes a camera 51, a flashlight 52 and a reflector 53, wherein the camera 51 is fixed on the rotary table 44, and the flashlight 51 is fixed on the horizontal slider 423 with the head facing down and is located on the horizontal beam slide Below the 42, a reflector 53 is fixedly arranged at a position 10 cm below the flashlight 51 lamp cap.

As shown in Figures 8 and 9, the irrigation subsystem 6 includes a nutrient solution bucket 61, an irrigation self-priming pump 62, a circulating self-priming pump 63, a connecting pipeline 64, a check valve 65, a first solenoid valve 66 and a second solenoid valve 67. Wherein, the inlet end of each root box irrigation pipe 25 is communicated with the connection pipe 64 through each branch pipe respectively, then communicates with the irrigation self-priming pump 62, the circulation self-priming pump 63 and the nutrient solution barrel 61 through the connection pipe 64, and the inlet The connecting pipe 64 at the end is provided with a check valve 65 and a first solenoid valve 66; the outlet ends of each box irrigation pipe 25 are respectively connected with the connecting pipe 64 through each branch pipe, and then connected with the nutrient solution barrel 61 through the connecting pipe 64 and the connecting pipe 64 at the outlet end is provided with a second solenoid valve 67 . When irrigation is not needed, the first electromagnetic valve 66 and the second electromagnetic valve 67 are in the closed state; when irrigation, the first electromagnetic valve 66 is in the open state, and the irrigation self-priming pump 62 is started to absorb nutrients from the nutrient solution bucket 61 liquid, moves to the root box irrigation pipes 25 of different root boxes 2 through the connecting pipe 64, and provides the nutrient solution for the plants; when the irrigation ends, the second electromagnetic valve 67 is opened, and the irrigation self-priming pump 62 is closed to ensure that the irrigation ends immediately; When circulating, the first solenoid valve 66 and the second solenoid valve 67 are opened at the same time, and the nutrient solution is circulated in the connecting pipe 64 by the circulating self-priming pump 63, while the root box irrigation pipe 25 does not produce water, and the nutrient solution is ensured by the circulation of the nutrient solution After the replacement, the concentration of the nutrient solution in the connecting pipe 64 is consistent.

As shown in FIG. 8 and FIG. 9 , the water return subsystem 7 includes a water tank 71 , a U-shaped tank 72 , a water tank 73 and a submersible pump 74 . Wherein, the bottom of each box frame 3 is all fixedly provided with a water tank 71, is used for containing the excess nutrient solution that is discharged in the drainage groove 26 of root box 2, and the two ends of water tank 71 are closed, and one end bottom is perforated, and installs Waterproof joint, waterproof joint connects water guide pipe 711 (can be a PE pipe) and is used for water guiding, and the bottom end of water guide pipe 711 is connected with filter cloth bag 712, is used for filtering nutrient solution. The U-shaped groove 72 is fixedly arranged on one side of the bottom of the main frame 1 and is positioned under the filter cloth bag 712 of the tank 71. One end of the U-shaped groove 72 is closed, and the other end is connected with the water tank 73 for discharging the recovered nutrient solution to Water tank 73; submersible pump 74 is arranged in the water tank 73 and communicates with the nutrient solution bucket 61, and the submersible pump 74 regularly pumps the recovered nutrient solution in the water tank 73 into the nutrient solution bucket 61 to realize the recycling of the nutrient solution.

In the above-described embodiment, an arched drainage groove 426 is fixedly arranged on the horizontal beam slide table 42 of the H-shaped frame 4 corresponding to the U-shaped groove 72, and the filter cloth bag 712 can slide past the arched drainage groove 426 to avoid Filter cloth bag 712 shakes water and wets mechanical parts.

In the above embodiment, the water tank 71 and the U-shaped tank 72 are made of PVC material, and the water tank 71 is 40 mm wide, 2100 mm long, and 15 mm high.

As shown in FIG. 10 and FIG. 11 , the shading subsystem 8 includes a shading module 81 between the top beam of the root box frame, a shading module 82 between the root box frames, and a shading module 83 at the side of the main frame. Wherein, the shading module 81 for the space between the top beams of the root box frame 3 is used for filling the space between the two top beams 31 at the top of the root box frame 3, specifically including three horizontal openings for filling the gap between the plants and the inner side of the top middle of the root box frame 3. Hole nitrile rubber 811, the connecting block 812 fixed on the top of the vertical beam 33 at the two ends of the root box frame 3 respectively, and the vertical perforated nitrile rubber 813 for sealing the gap between the connecting block 812 and the two top beams 31 ends. The shading module 82 between the root box frames is used for shading between the root box frames 3 and between the root box frame 3 and the main frame 1, including an n-type micro-shrinkage organ cover 821 and a suspension beam 822, and an n-type micro-shrink force organ cover The two ends of 821 are respectively fixedly connected with the top beams 31 of two box frames 3 or with the top beam 31 of one box frame 3 and the upper beam 11 of the main frame 1, and the sliding of the root box frame 3 can drive the n-type micro-shrink force The expansion and contraction of the organ cover 821; the two ends of the four suspension beams 822 are respectively fixed on the two upper beams 11 at the two ends of the top linear guide rail 14, and the suspension beams 822 are provided with a plurality of suspension rings 8221 and suspension hooks 8222. The organ cover 821 is suspended on the suspension beam 822 through the suspension ring 8221 and the hook 8222 to prevent the n-type micro-shrinkage organ cover 821 from sinking; thereby realizing the connection between two adjacent box frames 3 and between the root box frame 3 and the main frame. 1 shade between. The side shading module 83 of the main frame is used for shading around the main frame 1, and mainly adopts the side shading cloth 831 that is all sewn with waterproof zippers on the 3 sides, which can facilitate the disassembly and connection of the side shading cloth 831; The side shading cloths 831 around the main frame 1 are sewed together by waterproof zippers, so as to realize the shading around the main frame 1.

In the above embodiment, the n-type micro-shrinkage organ cover 821 includes a horizontal inline long organ cover 8211, a vertical inline organ cover 8212, an interleaved folded organ cover 8213, an inline long end plate 8214 and an L-shaped Connecting plate 8215; the horizontal one-shaped long bellows piece 8211 and the vertical one-shaped bellows piece 8212 are stitched and connected with the cross-folded bellows piece 8213 to form an n-shaped bellows unit, and multiple n-shaped bellows units are connected to each other to form n-shaped bellows Type organ cover unit group, which can increase the stretching range of the organ cover and reduce the external force required for stretching; the two ends of the inline long end plate 8214 are connected with the L-shaped connecting plate 8215 to form an n-shaped end plate, and the n-shaped organ cover unit The two ends of the stretching direction of the group are respectively connected with an n-type end plate, thereby forming an n-type micro-shrinkage force organ cover 821; The top beam 31 is fixedly connected.

In the above-mentioned embodiment, the outer sides of the two upper beams 11 provided with top linear guide rails 14 on the top of the main frame 1 are respectively fixed with long water boxes 19, and the long water boxes 19 are located on the vertical inline organ of the n-type micro-shrink force organ cover 821 Cover sheet 8212 bottom; add water to the long water box 19 to be higher than the bottom of the vertical inline organ cover sheet 8212, the outside of the long water box 19 is fixedly connected with the side shading cloth 831 to realize the top n-type micro-shrinkage force organ cover 821 and the two The connection of the side shading cloth 831 on each side can realize sealing and shading when the root box frame 3 and the n-type micro-shrinkage organ cover 821 slide, which solves the n-type micro-shrinkage of the side shading cloth 831 and bending. The organ cover 821 is directly connected to complex and difficult problems.

In the above-mentioned embodiment, the movement opening and closing control of the imaging subsystem 5, the irrigation subsystem 6 and the return water subsystem 7 are all controlled by an Arduino microcontroller, which can realize different irrigation volumes and Nutrient circulation.

When the root system high-throughput cultivation and automatic growth imaging system in a completely dark environment of the present invention is in use, first the H-shaped frame 4 slides to a certain distance from the first root box frame 3, and the horizontal beam slide 42 rises to The height of the camera 5 parallel to the middle of the root box 2, the camera 5 slides from left to right to the front of the first root box 2, and takes pictures of each root box 2 in turn; then the horizontal beam slide 42 descends to the bottom, H-shaped The frame 4 slides backwards to the middle of the two top beams 31 of the first root box frame 3, and the rotating arm 418 is driven by the stepping motor 416 to rotate vertically upwards, and then the H-shaped frame 4 slides forward, simultaneously driving The first root box frame 3 moves forward for a certain distance, and then the rotating arm 418 rotates to be vertically downward; At a certain distance from the root box frame 3, the camera 5 rotates 180°, and the horizontal beam slide 42 rises to the height where the camera 5 is parallel to the middle part of the root box 2, and the camera 5 takes pictures of the back of each root box 2 from left to right in turn; Afterwards, the H-shaped frame 4 slides forward to a certain distance from the second root box frame 3, the camera 5 rotates 180°, and starts to shoot the root box 2 on the second root box frame 3; and so on, all the root boxes are completed 2 for taking pictures.

The above-mentioned embodiments are only used to illustrate the present invention, and the structure, setting position and connection method of each component can be changed, and all equivalent transformations and improvements carried out on the basis of the technical solution of the present invention are not applicable. Should be excluded from the protection scope of the present invention.

Claims (10)

1. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation, which is characterized in that the system includes:

Main frame,

For cultivating the root box of plant root,

It is mounted on the root box frame that can be moved on the main frame and relative to the main frame,

It is mounted on the H-type rack that can be moved on the main frame and relative to the main frame,

Be mounted in the H-type rack and can relative to the imaging subsystems of the H-type gantry motion,

The irrigation subsystem for being mounted on the main frame and being connected with described box,

The return water subsystem for being mounted on the main frame and being connected with the irrigation subsystem, and

For covering the shading subsystem for realizing full dark situation inside whole system；

Wherein, multiple described boxes are removably mounted on described box frame；The imaging subsystems are used for described box In root system be imaged；The irrigation subsystem, which is used to provide nutrient solution for the plant in described box, realizes soilless cultivation； The return water subsystem is recycled for recycling the extra nutrient solution oozed out from described box.

2. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as described in claim 1 Sign is that the main frame includes more upper beams and underbeam, is connected between the upper beam and underbeam by vertical beam；It is parallel opposite Linear top guide rail and inside linear guide is fixedly installed in the top and inside of two upper beams respectively, and parallel opposite two The bottom linear guide and bottom ball screw parallel with the two inside linear guides are respectively set on the inside of the underbeam, Sliding is provided with top slide, inside block respectively in the linear top guide rail, inside linear guide and bottom linear guide And base slider；Described box frame is mounted on the main frame by the top slide and can be transported relative to the main frame It is dynamic；The H-type rack is mounted on the main frame by the inside block and base slider and can be relative to the main frame Frame movement.

3. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation as claimed in claim 1 or 2, It is characterized in that, described box includes centrally disposed culture body, the background cloth for being covered on culture body front and back two sides, difference Be arranged in it is described culture body left and right sides and bottom edge spacer bar, be arranged in the background cloth outside and with the spacer bar The transparent splint being fixedly connected and the root box irrigation pipe being arranged at the top of the culture body；It is described culture body thickness with it is described The consistency of thickness of spacer bar；The spacer bar of the spacer bar of the culture body bottom apart from the culture body and two sides Bottom a distance forms the rhone in described cassette bottom portion；Described box irrigation pipe is connected with the irrigation subsystem.

4. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 2 Sign is that described box frame includes that the top beam of two parallel intervals setting and the top beam are set in parallel in two top beams Between the bottom beam of underface being spaced and the vertical beam for being connected as one the top beam and bottom beam, the top beam, bottom beam and The loading space of described box is surrounded between vertical beam；The length of the top beam is more than or equal to the width of the main frame, passes through The both ends of the top beam are fixedly connected with the top slide of the main frame respectively, so that described box frame is mounted on On the main frame；The length of the bottom beam is less than the width of the main frame.

5. the cultivation of root system high throughput and automation growth imaging system under a kind of full dark situation as claimed in claim 2 or 4, It is characterized in that, the H-type rack includes the level that the vertical slide unit of two sides, both ends are slidably connected with the two vertical slide units respectively Crossbeam slide unit, the simultaneously pedestal that is fixedly connected with the bottom of the two vertical slide units and it is slidably arranged in the horizontal gird slide unit On turntable, the two vertical slide units and the horizontal gird slide unit constitute H-type, and the imaging subsystems are fixedly mounted on institute It states on turntable；The both ends of the pedestal are fixedly connected with two base sliders of the main frame respectively, and two is described vertical The top side of slide unit is fixedly connected with two inside blocks of the main frame respectively, so that the H-type rack can be opposite It is slided in the main frame；The horizontal gird slide unit can be relative to the H-type rack lifting carriage；The turntable can be opposite It is slided in the H-type rack level；The turntable can be rotated around itself 360 ° of vertical rotary shaft.

6. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 5 Sign is that the vertical slide unit includes vertical line guide rail and vertical ball screw, the vertical slipper of the vertical line guide rail It is connected with the down-feed screw nut of the vertical ball screw, the motor vertical driving of the vertical ball screw is described vertical Feed screw nut's movement, so that the vertical slipper be driven to slide relative to the vertical line guide rail；The horizontal gird slide unit Both ends be fixedly connected respectively with the vertical slipper on the vertical line guide rail of the two vertical slide units, it is described vertical Ball screw drives the vertical slipper movement to drive the horizontal gird slide unit lifting by the down-feed screw nut；It is described Horizontal gird slide unit includes horizontal linear guide rail and horizontal ball screw, the horizontal slider and the water of the horizontal linear guide rail The horizontal lead screw nut of flat ball screw is connected, and the horizontal motor of the horizontal ball screw drives the horizontal lead screw nut Movement, so that the horizontal slider be driven to slide relative to the horizontal linear guide rail；The bottom silk of the bottom ball screw Stem nut is fixedly connected with the base crossbeam of the pedestal, and the bottom motors of the bottom ball screw drive the bottom screw rod Nut movement, to drive the H-type housing slide；On the horizontal linear guide rail of the turntable and the horizontal gird slide unit Horizontal slider be fixedly connected, the horizontal ball screw drives the horizontal slider movement to drive the turntable relative to institute State the sliding of horizontal gird slide unit.

7. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 5 Sign is, the imaging subsystems include that the imaging device being fixed on the turntable, lamp cap are fixedly mounted facing downward Flash lamp below the horizontal gird slide unit and the reflector being fixed at below the flash lamp lamp cap.

8. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 3 Sign is that the irrigation subsystem includes by the sequentially connected nutrient solution tank of connecting pipe, irrigates self priming pump and circulation self-priming Pump；The input end of each described box irrigation pipe passes through lateral and the connecting pipe for irrigating self priming pump one end respectively It is connected, the outlet end of each described box irrigation pipe passes through the connection of each lateral and described nutrient solution tank one end respectively Pipeline is connected；Non-return valve and the first solenoid valve, institute are additionally provided in the connecting pipe of described box irrigation pipe input end It states and is additionally provided with second solenoid valve in the connecting pipe of root box irrigation pipe outlet end；

The return water subsystem include water tank, both ends closure and at one end the sink of bottom opening, one end closure and the other end and The U-type groove and the immersible pump for being arranged in the water tank and being connected with the irrigation subsystem that the water tank is connected；It is more A sink is respectively fixedly disposed at the bottom of each described box frame, and the aperture of the bottom of gullet is towards the same side； The aperture of the bottom of gullet connects aqueduct, and the aqueduct bottom end is connected with filter cloth bag；The U-type groove fixed setting In the main frame bottom side and positioned at the underface of the filter cloth bag.

9. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 4 Sign is that the shading subsystem includes antiglare module and main frame side between root box frame top beam interval antiglare module, root box frame Antiglare module；Wherein, between described box frame top beam interval antiglare module is used between two top beams of described box top of the trellis Every filling；Antiglare module is between described box frame and between described box frame and the main frame between described box frame Shading；Main frame side antiglare module is used for the shading of the main frame surrounding.

10. the cultivation of root system high throughput and automation growth imaging system, special under a kind of full dark situation as claimed in claim 9 Sign is that antiglare module includes the micro- convergent force organ cover of N-shaped and suspension hanging beam, the micro- convergent force wind of N-shaped between described box frame The both ends in the scalable direction of qin cover respectively with the top beam of the top beam of two described box framves or one described box frame and The upper beam of one main frame is fixedly connected, and the sliding of described box frame can drive the micro- convergent force organ cover of the N-shaped It is flexible；The more suspension hanging beams are fixed at the master with being parallel to the micro- scalable direction of convergent force organ cover of the N-shaped Frame roof is suspended in the suspension hanging beam in the middle part of the micro- convergent force organ cover of N-shaped；Main frame side photomask Block is sewed with the side black-out cloth of waterproof slide fastener using three Bian Shangjun, by by the side shading of the main frame surrounding Cloth is connected after being sutured by the waterproof slide fastener, realizes the shading of the main frame surrounding.