CN107024369A - To the method for cell pretreatment to be checked in the sticky body fluid of human body - Google Patents
To the method for cell pretreatment to be checked in the sticky body fluid of human body Download PDFInfo
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- CN107024369A CN107024369A CN201610066827.1A CN201610066827A CN107024369A CN 107024369 A CN107024369 A CN 107024369A CN 201610066827 A CN201610066827 A CN 201610066827A CN 107024369 A CN107024369 A CN 107024369A
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- cell
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention provides a kind of method to cell pretreatment to be checked, this method protects step in detail below：Step 1, viscosity is dissolved：Into the container for filling collection or the sample being collected into, goo resolve agent is added, goo resolve agent is the sodium-chloride water solution that the concentration configured is 0.2%~27%, sample viscosity is fully dissolved by mode of oscillation；Step 2, protect：The cell-protecting configured is added in above-mentioned goo resolve agent, makes to add the sample of goo resolve agent fully to merge with cell-protecting by vibration, completion processing；The cell-protecting is the mixture of formaldehyde, white wine or both, and the concentration of the formaldehyde of formaldehyde white spirit mixed thing is 3~30%, and the number of degrees of white wine are 35~85 degree (alcoholic strength).The method to cell pretreatment to be checked that the present invention is provided is more efficient, safe; pretreated cell to be checked is easier to extract, handle and detect; testing result is more accurate; and; cell-protecting is in terms of cell is protected; more have and relax keeping quality, beneficial to observation thereafter to intracellular more fine texture, analysis.
Description
Technical field
The present invention relates to medical detection method, particularly a kind of method pre-processed to cell to be checked and its agents useful for same.
Background technology
Medically, in the detection process to the cell to be checked such as the oncocyte that comes off, inflammatory cell, it is necessary to which first sampling sample, blood, stool to sample to be collected i.e. human body sticky body fluid such as phlegm, woman uterus carries out vanishing viscosity processing before being detected to cell to be checked.Traditional processing mode is all individually or after mixing to be directly added into the solution such as alcohol, glacial acetic acid, alkalies in the container for the sample for filling collection; making one bulk viscosity body fluid can not dissolve very well substantially; cell to be checked in the sample of collection can not well be separated and be originally used for protecting alcohol of cell etc.; do not play a part of not only protecting cell; easily cause on the contrary and lumpd in sample or form the shape that sticks together, cell is instead more wrapped up；And the addition of other sticky enzyme things of neutralizing, not only dissolve sample viscosity, while will also result in the neutralizing of cell；These will all influence follow-up detection.
The content of the invention
It is an object of the invention to provide a kind of method pre-processed to cell to be checked; the sample of collection is first pre-processed; make one bulk viscosity the body fluid such as sampling of phlegm, woman uterus, blood and stool sampl neutralizing and the protection of cell is carried out step by step; so that cell to be checked is sufficiently separated, cell to be checked can be more easily extracted, handles and detected.
In order to achieve the above object, the invention provides a kind of method to cell pretreatment to be checked, it is characterised in that this method protects step in detail below：
Step 1, viscosity is dissolved：Into the container for filling collection or the sample being collected into, goo resolve agent is added, goo resolve agent is the sodium-chloride water solution that concentration is 0.2%~27%；Make the viscosity fully neutralizing of sample by mode of oscillation；Human body viscosity body fluid is phlegm, woman uterus sampling, blood and stool sampl；
Step 2, protect：The cell-protecting configured is added in above-mentioned goo neutralizing reagent, by making to add the sample of goo resolve agent fully to merge with cell-protecting, completion processing；The cell-protecting is the mixture of formaldehyde, white wine and surfactant, and the concentration of formaldehyde of formaldehyde white spirit mixed thing is 3~30%, and white wine is 35~85 degree, and less than 3% surfactant can be also included in mixture.
Wherein, the sodium-chloride water solution of goo resolve agent is preferably the aqueous solution of 0.5%-5% sodium chloride；Edible salt, chemical industry or industrial salt may be selected in the sodium chloride, and the preferred salt of the sodium chloride is more convenient to be easy to get.
Wherein, the cell-protecting is the mixture of formaldehyde, white wine or both, and the concentration of formaldehyde of formaldehyde white spirit mixed thing is 3~30%, and white wine is 35~85 degree.
Wherein, sodium-chloride water solution is added in two times, the volume of the sodium chloride solution added first is more than the volume of people's bulk viscosity liquid, high vibration more than more than ten seconds, then sodium chloride solution is added again, its volume is more than more than 3 times that human body viscous liquid is accumulated, using supersonic oscillations so that it is fully dissolved.
Wherein, concentration of formaldehyde is 3-15%, and white wine is 35-45 degree.
Wherein, supersonic oscillations are carried out after cell-protecting is added more than 10 minutes, while 30~45 DEG C of heating is carried out, so that both fully fusions.
Further, also comprising less than 3% surfactant or the detergent or cleaning agent that deoil in cell-protecting so that the subsurface liquid of liquid foam fully dissolves and protected after processing.
The present invention is by adding goo resolve agent in the container to the sample for filling collection, and add in two times, the a small amount of resolve agent added first, high vibration more than more than ten seconds, then resolve agent is added again, its volume is more than more than 3 times that human body viscous liquid is accumulated, using supersonic oscillations and is mixed with 30~45 DEG C of heating, so that it is fully dissolved.By the abundant separate out of cell to be checked, add cytoprotection reagent and adequately protected, more efficient, safety, pretreated cell to be checked is easier to extract, handle and detect, and testing result is more accurate.
Embodiment
Introduced below is the embodiment as content of the present invention, and the content of the present invention is made further to illustrate below by embodiment.Certainly, the content of description following detailed description only for example different aspect of the invention, and should not be construed as limiting the scope of the invention.
Reference examples 1
In the container that the mixed liquor of glacial acetic acid and formalin is added directly into the sputum sample sheet for filling collection, vibration is sufficiently mixed it；At this moment it may be seen that the sample containing goo of collection can not fully dilute dissolving in above-mentioned mixed liquor, there are big block, big tablet, big bar and/or the shape thing that sticks together that can not dissolve out, these objects can be by it is desirable that obtained cell encapsulation to be checked, so as to influence accuracy of detection.Furthermore, this liquid is when delivering filtrating test, and big block, piece, bar and the shape thing that sticks together in liquid can block filter membrane, it is impossible to reach that we fully handle liquid to be checked, accurately detect the purpose of cell to be checked.Then testing agency is delivered, its result is as shown in Table 1.
Comparative example 2
In the container that the mixed liquor of vinegar and edible distillate spirit is added directly into the sputum sample sheet for filling collection, vibration is sufficiently mixed it；At this moment it may be seen that the sample containing goo of collection can not dilute dissolving completely in above-mentioned mixed liquor, can also there is a small amount of insoluble matter, these insoluble matters still can influence accuracy of detection to obtained cell encapsulation to be checked.Furthermore, this liquid is when delivering filtrating test, and a small amount of insoluble matter in liquid can block filter membrane once in a while, it is impossible to reaches that we fully handle liquid to be checked, accurately detects the purpose of cell to be checked.Then testing agency is delivered, its result is as shown in Table 1.
Embodiment
Configure goo resolve agent：Goo resolve agent is prepared with water and salt (in addition to salt, chemical industry, industrial sodium chloride can also), its concentration is reached 3%.The ratio of water and salt can be determined using the method calculated when preparing solution, so as to reach the concentration of needs, the concentration of solution can also be tested with Baume hydrometer, salt and/or water are added respectively, so as to reach the concentration of needs.
Cell-protecting is prepared with formaldehyde and white wine (number of degrees are 40 degree), the concentration of formaldehyde is 10%；Formaldehyde, white wine are defined by the concentration that purchased product is marked in itself, and their compatibility is calculated by the next formaldehyde of purchase, the concentration of white wine in itself when preparing solution, so as to reach the concentration of needs, form cell-protecting.
Step 1, viscosity is dissolved：Into the container for filling collection or the sample being collected into, it is sufficiently separated the cell in body fluid by way of vibration, sodium-chloride water solution is added in two times, the volume of the sodium chloride solution added first is more than the volume of people's bulk viscosity liquid, high vibration more than more than ten seconds, then sodium chloride solution is added again, its volume is more than 3 times that human body viscous liquid is accumulated, using supersonic oscillations more than 10 minutes, at this moment, it may be seen that the sample containing goo of collection can fully dilute dissolving in above-mentioned mixed liquor, there is no big block, piece, bar and/or the shape thing that sticks together that can not dissolve out, so as to not interfere with accuracy of detection.This liquid is when delivering filtrating test, and we can adequately protect liquid to be checked processing, reach the purpose for accurately detecting cell to be checked.
Step 2, protect：The cell-protecting configured is added in goo neutralizing reagent, supersonic oscillations are carried out after cell-protecting is added more than 10 minutes, so that both fully fusions, so as to reach the protection to cell to be checked；Then the sample containing cell to be checked protected is delivered into testing agency, it is more easily extracted, handle and detect cell to be checked when being detected.
Embodiment 2-4 is carried out by the change in concentration for changing goo resolve agent and cell-protecting respectively, the sample that the method processing of the sample and reference examples 1-2 containing cell to be checked obtained after being handled using the embodiment 1~4 of the above method is obtained is compared as follows shown in table：
Table one：Embodiment 1~4 and the result of the method respective pretreatment cell to be checked of reference examples
As seen from the above table; pass through the method for the present invention; first cell to be checked is separated; the purpose for after salt solution sufficiently vibrate and fully being dissolved with reaching especially through adding; then add cell-protecting and carry out protection processing; and by vibration fusion; big block, piece, bar or the insoluble shape thing that sticks together are free of in the sample containing cell to be checked after this method processing; it can fully dissolve and protect cell to be checked; make its survival rate higher; extract, handle, detection is easier, detection accuracy is greatly improved, and avoiding blocking the filter membrane examined and filtered.
Although the embodiment to the present invention gives detailed description and explanation above; but it should be noted that; we can carry out various equivalent changes and modification according to the conception of the present invention to above-mentioned embodiment;, all should be within protection scope of the present invention during the spirit that function produced by it is still covered without departing from specification.
Claims (5)
1. a kind of method of cell pretreatment to be checked in sticky body fluid to human body, it is characterised in that this method includes
Step in detail below：
Step 1, viscosity is dissolved：Into the container for filling collection or the sample being collected into, goo neutralizing is added
Agent, goo resolve agent is the sodium-chloride water solution that concentration is 0.2%~27%；Sample is made by mode of oscillation
The fully neutralizing of this viscosity；Human body viscosity body fluid is phlegm, woman uterus sampling, blood and stool sampl；
Step 2, protect：The cell-protecting configured is added in above-mentioned goo neutralizing reagent, passed through
Make to add the sample of goo resolve agent fully to merge with cell-protecting, completion processing；The cytoprotection
Agent is the mixture of formaldehyde, white wine or both, and the concentration of formaldehyde of formaldehyde white spirit mixed thing is 3~30%,
White wine is 35~85 degree.
2. the method for claim 1, wherein sodium-chloride water solution is added in two times, add first
The volume of sodium chloride solution is more than the volume of people's bulk viscosity liquid, high vibration more than more than ten seconds, Ran Houzai
Secondary addition sodium chloride solution, its volume is more than more than 3 times that human body viscous liquid is accumulated, is shaken using ultrasonic wave
Swing so that it is fully dissolved.
3. the method for claim 1, wherein concentration of formaldehyde is 3-15%, white wine is 35-45 degree.
4. the method as described in claim 1-3, wherein, carry out supersonic oscillations after cell-protecting is added
More than 10 minutes, heated simultaneously in sonic oscillation, temperature control is at 30~45 DEG C so that both fully melt
Close.
5. the method as described in claim 1-3, wherein, also included in cell-protecting less than 3% surface
Activating agent or the detergent or cleaning agent deoiled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610066827.1A CN107024369A (en) | 2016-02-01 | 2016-02-01 | To the method for cell pretreatment to be checked in the sticky body fluid of human body |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610066827.1A CN107024369A (en) | 2016-02-01 | 2016-02-01 | To the method for cell pretreatment to be checked in the sticky body fluid of human body |
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| CN107024369A true CN107024369A (en) | 2017-08-08 |
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| CN201610066827.1A Pending CN107024369A (en) | 2016-02-01 | 2016-02-01 | To the method for cell pretreatment to be checked in the sticky body fluid of human body |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881114A (en) * | 2021-01-12 | 2021-06-01 | 桂林优利特医疗电子有限公司 | Quality control composition for analyzing visible components of vaginal secretion and preparation method thereof |
| CN114397141A (en) * | 2021-12-10 | 2022-04-26 | 连云港市东方医院 | Department of neurology is clinical with getting liquid device |
| CN117740741A (en) * | 2024-02-20 | 2024-03-22 | 成都市龙泉驿区中医医院 | Clinical laboratory blood analysis detecting system |
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| CN201379998Y (en) * | 2008-08-06 | 2010-01-13 | 马铁军 | Double inner combined device for obtaining final isolates through multilayer separation and fast regulation |
| CN201807225U (en) * | 2010-07-16 | 2011-04-27 | 张曼 | Urine separating and enriching device |
| CN102914466A (en) * | 2011-08-01 | 2013-02-06 | 马铁军 | Method for pretreating to-be-detected cells and reagents used by same |
| CN203299039U (en) * | 2013-06-14 | 2013-11-20 | 南京奕昕生物科技有限公司 | Body fluid sample treatment tube |
| CN103674673A (en) * | 2013-12-20 | 2014-03-26 | 马铁军 | Method and device for preprocessing discharged sample |
-
2016
- 2016-02-01 CN CN201610066827.1A patent/CN107024369A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201379998Y (en) * | 2008-08-06 | 2010-01-13 | 马铁军 | Double inner combined device for obtaining final isolates through multilayer separation and fast regulation |
| CN201807225U (en) * | 2010-07-16 | 2011-04-27 | 张曼 | Urine separating and enriching device |
| CN102914466A (en) * | 2011-08-01 | 2013-02-06 | 马铁军 | Method for pretreating to-be-detected cells and reagents used by same |
| CN203299039U (en) * | 2013-06-14 | 2013-11-20 | 南京奕昕生物科技有限公司 | Body fluid sample treatment tube |
| CN103674673A (en) * | 2013-12-20 | 2014-03-26 | 马铁军 | Method and device for preprocessing discharged sample |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881114A (en) * | 2021-01-12 | 2021-06-01 | 桂林优利特医疗电子有限公司 | Quality control composition for analyzing visible components of vaginal secretion and preparation method thereof |
| CN114397141A (en) * | 2021-12-10 | 2022-04-26 | 连云港市东方医院 | Department of neurology is clinical with getting liquid device |
| CN117740741A (en) * | 2024-02-20 | 2024-03-22 | 成都市龙泉驿区中医医院 | Clinical laboratory blood analysis detecting system |
| CN117740741B (en) * | 2024-02-20 | 2024-05-07 | 成都市龙泉驿区中医医院 | Clinical laboratory blood analysis detecting system |
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Application publication date: 20170808 |
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