CN107022490A - A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof - Google Patents
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof Download PDFInfo
- Publication number
- CN107022490A CN107022490A CN201610065545.XA CN201610065545A CN107022490A CN 107022490 A CN107022490 A CN 107022490A CN 201610065545 A CN201610065545 A CN 201610065545A CN 107022490 A CN107022490 A CN 107022490A
- Authority
- CN
- China
- Prior art keywords
- solution
- impurity
- preparation
- coccidian oocyst
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000224483 Coccidia Species 0.000 title claims abstract description 26
- 210000003250 oocyst Anatomy 0.000 title claims abstract description 24
- 239000012535 impurity Substances 0.000 title claims abstract description 21
- 238000000605 extraction Methods 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 22
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052901 montmorillonite Inorganic materials 0.000 claims abstract description 11
- 241000218176 Corydalis Species 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 8
- 230000009467 reduction Effects 0.000 claims description 7
- 238000013019 agitation Methods 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 239000008213 purified water Substances 0.000 claims description 6
- 239000004695 Polyether sulfone Substances 0.000 claims description 5
- 229920006393 polyether sulfone Polymers 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 3
- -1 Ether sulfone Chemical class 0.000 claims 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 229960005486 vaccine Drugs 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 2
- 238000007654 immersion Methods 0.000 abstract description 2
- 238000012856 packing Methods 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 239000008187 granular material Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 41
- 239000002775 capsule Substances 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical class O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The present invention relates to a kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof and preparation method thereof, the solution contains：5-15 parts of montmorillonite, 10-30 parts of corydalis tuber, 5-10 parts of activated carbon；10-45 parts of Rhizoma Sparganii, 10-40 parts of the radix paeoniae rubrathe, are made through processes such as wash, immersion, decoction, concentration, alcohol precipitation, filtering, packing.The solution reaches the degree of purity of vaccine by adsorbing the effect such as impurity unnecessary in excrement, fat granule, cellulose.
Description
Technical field
The invention belongs to veterinary biologics field, especially a kind of reduction excrement extraction method coccidian oocyst is miscellaneous
Solution of matter and preparation method thereof.
Background technology
Coccidia is to seriously endanger the important parasitic protozoa of a class that grows of poultry, in being also all chickens diseases
One of economic loss the most serious disease.Treatment of the China to global-worm illness in recent years increasingly uses coccidia
Vaccine is prevented and treated, and the extracting method of coccidian oocyst is a variety of, in excrement extract, organize in extract all be can
Capable, if the extract yield that selection tissue carries out egg capsule is low, and need to carry out inoculated chicken group
Slaughter, do not meet animal welfare, and easily scattered poison causes environmental pollution.Coccidian oocyst is extracted from excrement,
The coccidial vaccine impurity that egg capsule making is extracted in time saving, saving, environmental protection, but excrement is more, and
The easy cooperating microorganisms of impurity, microorganism and coccidian oocyst contention nutrient, cause the shelf-life of coccidial vaccine
Reduction, thus research it is a kind of reduce excrement extraction method coccidian oocyst impurity be study one of worm vaccine it is new
Developing direction.
The content of the invention
Present invention aims at provide it is a kind of reduce reduction excrement extraction method coccidian oocyst impurity solution and its
Preparation method.The weight proportion of the solution component is respectively：5-15 parts of montmorillonite, 10-30 parts of corydalis tuber,
5-10 parts of activated carbon；10-45 parts of Rhizoma Sparganii, 10-40 parts of the radix paeoniae rubrathe, through wash, immersion, decoction, concentration,
The processes such as alcohol precipitation, filtering, packing are made.Solution is by the effect such as flocculation, absorption, scattered in excrement
Coccidian oocyst separated, purified, it is the saturated saline floatation that compares, sucrose solution floating method, full
The egg capsule liquid collected with Adlerika floating method, chromatosulfuric acid partition method etc. is pure, clarity is high
The solution of excrement extraction method coccidian oocyst impurity is reduced it is another object of the present invention to provide a kind of
And preparation method thereof.
The present invention is achieved by the following technical solutions：
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the solution component
Weight proportion is respectively：
5-15 parts of montmorillonite；
10-30 parts of corydalis tuber；
5-10 parts of activated carbon；
10-45 parts of Rhizoma Sparganii；
10-40 parts of the radix paeoniae rubrathe.
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the preparation work of solution
Skill step is as follows：
(1) Rhizoma Sparganii, the radix paeoniae rubrathe are weighed successively according to weight proportion, traditional Chinese medicine extraction is put into after cleaning demulcen drying
In tank, by weight 1:1 adds purified water, after soaking 1 hour at room temperature, is further continued for being heated to water
Boiling is decocted 1 hour, and extract solution 0.45um polyether sulfone filter element filterings are obtained into solution A, storage to Agitation Tank
In；
(2) purified water of 3 times of weight of inventory is added into Chinese medicine extracting tank, is decocted again after being heated to boiling
Boil 0.5 hour, Agitation Tank inner storage tank is sucked after extract solution is filtered, and first time extract solution merges, and obtains
Solution B；
(3) montmorillonite, corydalis tuber, activated carbon are added to merge in liquid storage tank in step (2) and adds 3
The water of times weight, stirs, and precipitates, and sinking naturally collects supernatant C after 10 hours, standby；
(4) 0.1% merthiolate is added to step (3) resulting solution C, with 0.22um polyether sulfone filter cores
Filtering, you can obtain the solution that a kind of reduction chicken manure extracts coccidian oocyst impurity, be directly entered by pipeline
Bottle placer.
Advantages of the present invention and beneficial effect are：
1. activated carbon by adsorb bulky grain albumen in excrement, impurity, microorganism, can also be molten to egg capsule
Liquid is decolourized, and solution is more clarified；
2. the mesh of montmorillonite 800 can allow excrement and solution to mix evenly, it is in the excrement after dissolving mixed
Outstanding state, allows excrement and coccidian oocyst to be quickly completely separated.
3. the present invention has no toxic side effect, safe and convenient to use.
4. in the whole preparation process of the present invention, produced discarded object does not pollute the environment, it is easy to handle.
5. Rhizoma Sparganii, the radix paeoniae rubrathe can also reduce in excrement microbial survival there is provided nutrition needed for coccidian oocyst into
Point.
Embodiment
The present invention is described in further detail by following examples.It should be noted that：Following embodiments are explanations
Property, it is not limited, it is impossible to limit protection scope of the present invention with following embodiments.
Montmorillonite, corydalis tuber, activated carbon, Rhizoma Sparganii, the radix paeoniae rubrathe in instant component can be commercially
It is commercially available.
Embodiment 1
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the solution component
Optimal weight proportion is respectively：Montmorillonite 5kg, corydalis tuber 10kg, activated carbon 5kg, Rhizoma Sparganii 10kg,
Radix paeoniae rubrathe 10kg.
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the solution prepares work
Skill step is as follows：
(1) Rhizoma Sparganii, the radix paeoniae rubrathe are weighed successively according to weight proportion, traditional Chinese medicine extraction is put into after cleaning demulcen drying
In tank, by weight 1:1 adds purified water, after soaking 1 hour at room temperature, is further continued for being heated to water
Boiling is decocted 1 hour, and extract solution 0.45um polyether sulfone filter element filterings are obtained into solution A, storage to Agitation Tank
In；
(2) purified water of 3 times of weight of inventory is added into Chinese medicine extracting tank, is decocted again after being heated to boiling
Boil 0.5 hour, Agitation Tank inner storage tank is sucked after extract solution is filtered, and first time extract solution merges, and obtains
Solution B；
(3) montmorillonite, corydalis tuber, activated carbon are added to merge in liquid storage tank in step (2) and adds 3
The water of times weight, stirs, and precipitates, and sinking naturally collects supernatant C after 10 hours, standby；
(4) 0.1% merthiolate is added to step (3) resulting solution C, with 0.22um polyether sulfone filter cores
Filtering, you can obtain a kind of solution of reduction excrement extraction method coccidian oocyst impurity, be directly entered by pipeline
Bottle placer.
Embodiment 2
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the solution component
Optimal weight proportion is respectively：Montmorillonite 15kg, corydalis tuber 30kg, activated carbon 10kg, Rhizoma Sparganii 45kg,
Radix paeoniae rubrathe 40kg.
The step be the same as Example 1 of its preparation method.
Embodiment 3
A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, the solution component
Optimal weight proportion is respectively：Montmorillonite 10kg, corydalis tuber 15kg, activated carbon 8kg, Rhizoma Sparganii 25kg,
Radix paeoniae rubrathe 30kg.
The step be the same as Example 1 of its preparation method.
Clinical practice
Experiment description：SPF healthy chicks, 10 ages in days, are randomly divided into 4 groups by 100, every group 25,
10000/ml egg capsules are inoculated with, per oral inoculation 1ml/ only, 7 days after inoculation, collects excrement, excrement
By 1：20 ratios are separately added into saturated brine, saturation sucrose solution, solution of the present invention, saturation magnesium sulfate
Solution, after stirring 10 minutes, is filtered, collects filtrate, and filtrate is centrifuged 1800r/ minutes, existed respectively
0h, 12h, 24h, 48h observe result, collect egg capsule liquid and carry out comparing result such as following table：
The table present invention and the Contrast on effect of traditional extraction egg capsule solution
Conclusion：Present invention group clarification is bright, meets solution requirement.
Claims (2)
1. a kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof, its feature
It is：The component and its ratio of weight and number of the solution be：
2. a kind of a kind of reduction excrement extraction method coccidian oocyst impurity as claimed in claim 1
Solution and preparation method thereof, it is characterised in that：The step of preparation method is：
(1) Rhizoma Sparganii, the radix paeoniae rubrathe are weighed successively according to weight proportion, is cleaned in being put into after demulcen drying
In medicine extractor, by weight 1:1 adds purified water, after soaking 1 hour at room temperature, then
Continue to be heated to boiling water decoction 1 hour, extract solution 0.45um polyether sulfone filter element filterings are obtained
Solution A, is stored into Agitation Tank；
(2) purified water of 3 times of weight of inventory is added into Chinese medicine extracting tank, boiling is heated to
Decoct again afterwards 0.5 hour, Agitation Tank inner storage tank is sucked after extract solution is filtered, and carry for the first time
Take liquid to merge, obtain solution B；
(3) montmorillonite, corydalis tuber, activated carbon are added in step (2) and merged in liquid storage tank
The water of 3 times of weight is added, is stirred, is precipitated, is collected after sinking 10 hours naturally
Clear liquid C, it is standby；
(4) 0.1% merthiolate is added to step (3) resulting solution C, it is poly- with 0.22um
Ether sulfone filter element filtering, you can obtain a kind of solution of reduction excrement extraction method coccidian oocyst impurity,
Bottle placer is directly entered by pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610065545.XA CN107022490B (en) | 2016-01-30 | 2016-01-30 | Solution for reducing impurities of coccidian oocysts extracted from excrement and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610065545.XA CN107022490B (en) | 2016-01-30 | 2016-01-30 | Solution for reducing impurities of coccidian oocysts extracted from excrement and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107022490A true CN107022490A (en) | 2017-08-08 |
| CN107022490B CN107022490B (en) | 2020-11-20 |
Family
ID=59524999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610065545.XA Active CN107022490B (en) | 2016-01-30 | 2016-01-30 | Solution for reducing impurities of coccidian oocysts extracted from excrement and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107022490B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652315A (en) * | 2018-11-23 | 2019-04-19 | 佛山市正典生物技术有限公司 | A method of improving pig coccidian oocyst separative efficiency and cleanliness |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046533A1 (en) * | 1997-04-14 | 1998-10-22 | Zenon Environmental, Inc. | Process for purifying water |
| CN102951697A (en) * | 2012-11-28 | 2013-03-06 | 大连东泰产业废弃物处理有限公司 | Method for treating electroplating wastewater |
| CN103881913A (en) * | 2014-03-07 | 2014-06-25 | 广东省农业科学院动物卫生研究所 | Industria production method for separating and purifying chicken eimeria coccidium oocyst |
-
2016
- 2016-01-30 CN CN201610065545.XA patent/CN107022490B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998046533A1 (en) * | 1997-04-14 | 1998-10-22 | Zenon Environmental, Inc. | Process for purifying water |
| CN102951697A (en) * | 2012-11-28 | 2013-03-06 | 大连东泰产业废弃物处理有限公司 | Method for treating electroplating wastewater |
| CN103881913A (en) * | 2014-03-07 | 2014-06-25 | 广东省农业科学院动物卫生研究所 | Industria production method for separating and purifying chicken eimeria coccidium oocyst |
Non-Patent Citations (1)
| Title |
|---|
| 朱冠等: "用改良韦氏法纯化鸡球虫卵囊", 《动物学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652315A (en) * | 2018-11-23 | 2019-04-19 | 佛山市正典生物技术有限公司 | A method of improving pig coccidian oocyst separative efficiency and cleanliness |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107022490B (en) | 2020-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101703567A (en) | Preparation method for traditional Chinese medicine composition for improving immunity of livestock and poultry and use thereof | |
| CN102614499B (en) | Yak skin collagen compound traditional Tibetan medicine with anti-fatigue effect and preparation process thereof | |
| CN104367987B (en) | Formulation of astragalus root for animals and preparation method thereof | |
| CN101618140A (en) | Chinese veterinary drug injection for preventing and treating porcine reproductive and respiratory syndrome (PRRS) and preparation process thereof | |
| CN101744924A (en) | Multiple-effect Chinese herbal medicine compound of bacterial diseases of turbot | |
| CN103860616A (en) | Manyprickle Acathopanax Root extrat, and preparation method and application thereof | |
| CN111388613A (en) | Traditional Chinese veterinary medicine astragalus root compound particles for promoting growth and enhancing immunity | |
| CN102225146B (en) | Formula and production technology of traditional Chinese medicine injection for controlling bird flu | |
| CN102813850B (en) | Traditional Chinese medicine composition for preventing and treating poultry coccidiosis and preparation method of traditional Chinese medicine composition | |
| CN107022490A (en) | A kind of solution for reducing excrement extraction method coccidian oocyst impurity and preparation method thereof | |
| CN111377898A (en) | Preparation process of cranberry extract procyanidine | |
| CN110432404B (en) | Tilapia feed additive for reducing proinflammatory response and enhancing immune response and preparation method and application thereof | |
| CN105462793B (en) | A kind of healthy medicated wine its preparation method | |
| CN101879209A (en) | Oral liquid for strengthening healthy energy and relieving internal heat or fever for animals | |
| CN103720932A (en) | Porcine Chinese medicinal composition as well as preparation method and application thereof | |
| CN106071325A (en) | A kind of sea duck feed additive and preparation method thereof | |
| CN110664876B (en) | Extraction method of polyphenol component in Chinese date fruit drop | |
| CN1201745C (en) | Medicine combination contg. effective part of astragalus root | |
| CN107494918A (en) | A kind of firefly nutrient solution of health and preparation method thereof | |
| CN110302307B (en) | A kind of medicinal wine for nourishing yin and beautifying lady and preparation method thereof | |
| CN107510797B (en) | Method for extracting total flavonoids from bamboo leaves | |
| CN105646725A (en) | Application of enteromorpha's polysaccharide for preparing livestock and poultry immunopotentiator | |
| CN117362415A (en) | Poecilobdella manillensis emetic liquid and application thereof in large-scale hirudin extraction | |
| CN110448586A (en) | A kind of Astragalus Root P.E and its preparation method and application | |
| CN110934897A (en) | Preparation method of medicine for treating cerebrovascular disease sequelae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |