CN107018903A - A kind of implantation methods of dendrobium candidum - Google Patents
A kind of implantation methods of dendrobium candidum Download PDFInfo
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- CN107018903A CN107018903A CN201710341418.2A CN201710341418A CN107018903A CN 107018903 A CN107018903 A CN 107018903A CN 201710341418 A CN201710341418 A CN 201710341418A CN 107018903 A CN107018903 A CN 107018903A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
Sprouted the invention discloses a kind of implantation methods of dendrobium candidum, including A, seed, B, seedling culture, C, seedling replanting, D, management.When the inventive method plants dendrobium candidum, the dendrobium candidum quality better finally plucked, active constituent content is high, particularly the height of the content of polysaccharide.
Description
Technical field
The present invention relates to a kind of cultural method of dendrobium candidum, the extraction of the high dendrobium candidum of particularly a kind of polyoses content
Method.
Background technology
Dendrobium candidum nickname iron sheet orchid, bracketplant, Yunnan iron sheet, iron sheet bracketplant, are orchid family Dendrobium herbaceos perennials,
Dendrobium candidum is the top grade of Shihu " medicinal materials, have the laudatory title of " gold in Chinese celestial grass-medicine ", is used as medicine with stem, contains polysaccharide, stone
The active ingredients such as dry measure used in former times alkali, alkaloid, with nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, wet one's whistle improving eyesight the effects such as, it is antitumor, improve human body
In terms of immunity, treatment enterogastric diseases, anti-aging, anti-oxidant, platelet aggregation-against, reduction blood glucose, treatment cataract
There is good efficacy.
Modern pharmacology research shows that dendrobium candidum has humidification to non-specific and specific immunity, anti-swollen
In terms of the treatment of knurl, anti-oxidant, reduction blood glucose and cataract, pharmacological action is notable.Its chemical composition is more complicated, it has been determined that
Type of compounds mainly have：Alkaloids, polysaccharide, luxuriant and rich with fragrance class, bibenzyl parent nucleus class, wherein alkaloids, polysaccharide pharmacology are lived
Property is more notable.Polysaccharide constituents are the important substance basis that medicinal dendrobium plays immunocompetence effect, Dendrobium officinale polysaccharide energy
Peripheral leukocytes are significantly improved, promotes lymphocyte to produce migration inhibition factor, immunity of organisms can be effectively improved.2010 editions
《Pharmacopeia》The quality standard of dendrobium candidum has been recorded first, and assay is carried out to polysaccharide and mannose.
As the health-care efficacy of dendrobium candidum is widely recognized and received by people, dendrobium officinale far can not expire
The demand of sufficient people.People have begun to use in artificial cultivation dendrobium candidum, the cultural method of dendrobium candidum, and culture medium is iron
Skin stem of noble dendrobium conditions on which persons or things depend for existence, different culture raw material and with can be to the growth of dendrobium candidum, yield and active component etc.
Produce important influence.The culture medium of dendrobium candidum and existing cultural method, are unfavorable for the iron sheet stone of output in the prior art
The quality guarantee of dry measure used in former times.Particularly it cannot be guaranteed that in dendrobium candidum polysaccharide content so that polysaccharide in the dendrobium candidum finally given
Content is low.
The content of the invention
The purpose of the present invention, is to provide a kind of implantation methods of dendrobium candidum, during the inventive method plantation dendrobium candidum, most
The dendrobium candidum quality better plucked eventually, active constituent content is high, particularly the height of the content of polysaccharide.
What the present invention was realized in.A kind of implantation methods of dendrobium candidum, including A, seed are sprouted, B, seedling culture,
C, seedling replanting, D, management.
The implantation methods of foregoing dendrobium candidum, specifically include following steps：
(1) seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection is used on superclean bench
After 5-15min, then with aseptic water washing 3-4 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, in daytime
Temperature is 20-25 DEG C, 18-20 DEG C of night temperature, light culture, until seed is sprouted；
(2) seedling culture：After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, at 23-28 DEG C,
Daily illumination 9-10h under light intensity 1500-2500lx, until dendrobium candidum height of seedling is 5-8cm；
(3) seedling replanting：Transplanting time is the 4-6 months, and height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling with clear water
The culture medium of root, then the sterilization of immersion root is diluted with 0.3-0.5% potassium permanganate, it is 10-15cm dendrobium candidum seedlings by line-spacing
Plant in matrix, it is ensured that matrix covers root system, root system is contacted with matrix；
(4) manage：
A. water：Using the mode that sprays, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 6-9 months
5-6 days 1 time, 2-3 days 9-12 months 1 time；
B. summer carries out sunshade using the sunshade net of 70-80% sunshade degree；
C. Temperature and Humidity Control：It is 60-70% to control air humidity, and temperature is 20-25 DEG C；
D. chemical fertilizer topdresses and applied in three times, for the first time 2-3 months after seedling replanting, second seedling replanting it
4-5 months afterwards, for the third time 6 months after seedling replanting；
(5) March November to next year, harvesting, you can.
In the implantation methods of foregoing dendrobium candidum, the step (1), the germination medium is main by by weight
10-20 parts of 5-15 parts of 3-6 parts of sucrose, agar, the red sage root of calculation and tuber of dwarf lilyturf 10-20 part are made as follows：The red sage root and the tuber of dwarf lilyturf add
The 10-12 times of decocting measured is boiled after 2-3h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar,
Produce.
The implantation methods of foregoing dendrobium candidum, main 5 parts of sucrose, the fine jade by calculating by weight of the germination medium
15 parts of 10 parts of fat, 15 parts of the red sage root and the tuber of dwarf lilyturf are made as follows：The red sage root and the tuber of dwarf lilyturf add the decocting of 11 times of amounts to boil after 2.5h, mistake
Filter, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced.
In the implantation methods of foregoing dendrobium candidum, the step (2), the proliferated culture medium is main by by weight
3-6 parts of the sucrose of calculation, 10-20 parts of sweet potato mash, 5-15 parts of soybean residue, 5-15 parts of ox Teng, 10-20 parts of the bark of eucommia, 5-15 parts of asarum, solely
10-20 parts of living 5-15 parts, 10-20 parts of Radix Angelicae Sinensis and Chinese ephedra are made as follows：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and fiber crops
Huang plus the 10-12 times of decocting measured are boiled after 2-3h, are filtered, are taken filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato
Mud, soybean residue are mixed, and are produced.
The implantation methods of foregoing dendrobium candidum, main 5 parts of the sucrose, red by calculating by weight of the proliferated culture medium
15 parts of 15 parts of mashed potatoes, 10 parts of soybean residue, 10 parts of ox Teng, 15 parts of the bark of eucommia, 10 parts of asarum, 10 parts of levisticum, 15 parts of Radix Angelicae Sinensis and Chinese ephedra are pressed
Following methods are made：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter
Slag, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash and soybean residue, produced.
In the implantation methods of foregoing dendrobium candidum, the step (3), the main sugarcane by calculating by weight of the matrix
Sugared 3-6 parts, 10-20 parts of sweet potato mash, 10-20 parts of mashed potatoes, 5-15 parts of cornmeal mush, 5-15 parts of soybean residue, 10-20 parts of cultivated land, pellet
10-20 parts of 5-15 parts of skin, 10-20 parts of kuh-seng, 5-15 parts of the fruit of glossy privet and evodia rutaecarpa are made as follows：Cultivated land, the root bark of tree peony, hardship
Ginseng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil after 2-3h, filter, take filter residue, dry, pulverize into coarse granule, thick
Grain is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, is produced.
The implantation methods of foregoing dendrobium candidum, the matrix it is main by calculate by weight 5 parts of sucrose, sweet potato mash 15
Part, 15 parts of mashed potatoes, 10 parts of cornmeal mush, 10 parts of soybean residue, 15 parts of cultivated land, 10 parts of the root bark of tree peony, 15 parts of kuh-seng, 10 parts of the fruit of glossy privet and Wu
15 parts of the fruit of medicinal cornel is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil 2-3h
Afterwards, filter, take filter residue, dry, pulverize into coarse granule, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue
It is even, produce.
In the implantation methods of foregoing dendrobium candidum, the step (4), the chemical fertilizer is nitrogenous fertilizer, is calculated by N, amount of application
For 138-207kg/hm2, apply fertilizer in two times, be for the first time seed manure, amount of application accounts for the 2/10 of total N content, second is to topdress, and is applied
Amount accounts for the 3/10 of total N content, and for the third time to topdress, amount of application accounts for the 5/10 of total N content.
Applicant has carried out substantial amounts of experimental study to the present invention, and part Experiment is as follows：
The polyoses content of experimental example 1 investigates
1 materials and methods
1.1 material
1.1.1 dendrobium candidum 1：Planted by embodiment 1, pluck, produce.
1.1.2 dendrobium candidum 2：Planted by embodiment 1, the red sage root and the tuber of dwarf lilyturf, Multiplying culture are free of in germination medium
Be free of in base and cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and Wu Zhu are free of in ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra, matrix
Cornel, remaining method be the same as Example 1.
1.2 instruments and reagent
Dextrose standard sample [glucose of D mono- (+) one]：KAYON companies；Methanol (chromatographically pure)：Close europeanized of Tianjin section
Reagent Co., Ltd；Remaining reagent is that analysis is pure；Phenol：Shanghai Ling Feng chemical reagent Co., Ltd；Sulfuric acid：Insult peaking in Shanghai
Learn reagent Co., Ltd.
2 methods
The preparation of 2.1 need testing solutions
2.1.1 the preparation of need testing solution 1：
Dendrobium candidum of the present invention (dendrobium candidum 1) sample is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, took
Medicinal powder after filter, volatilizes solvent, and add water 2000mL, is heated to reflux 2 hours, lets cool, is transferred in 250mL measuring bottles, with a small amount of
Moisture time washing container, washing lotion is incorporated in same measuring bottle, adds water to scale, shake up, and filters, and precision measures subsequent filtrate 2mL, put
In 15mL centrifuge tubes, precision adds 95% ethanol, shakes up, and refrigerates 1 hour, takes out, and centrifuges 20 minutes, abandoning supernatant, precipitation
Plus hot water dissolving, it is transferred in 25mL measuring bottles, lets cool, add water to scale, shake up, produces need testing solution 1.
2.1.2 the preparation of need testing solution 2
The sample of dendrobium candidum 2 is taken, 50 DEG C is put in baking oven, dry, pulverize, No. three sieves is crossed, takes medicinal powder after filtering, volatilize
Solvent, add water 2000mL, is heated to reflux 2 hours, lets cool, is transferred in 250mL measuring bottles, with a small amount of moisture time washing container, washes
Liquid is incorporated in same measuring bottle, adds water to scale, is shaken up, and filtration, precision measures subsequent filtrate 2mL, put in 15mL centrifuge tubes, and precision adds
Enter 95% ethanol, shake up, refrigerate 1 hour, take out, centrifuge 20 minutes, abandoning supernatant, precipitation plus hot water dissolving are transferred to
In 25mL measuring bottles, let cool, add water to scale, shake up, produce need testing solution 2.
The preparation of 2.2 reference substance solutions
It is appropriate to the DEXTROSE ANHYDROUS of constant weight that precision weighs 105 DEG C of dryings, adds water and 99mg/L solution is made, standby.
It is prepared by 2.3 standard curves
Precision measure reference substance solution 0.2,0.4,0.6,0.8,1.0mL put in 10mL tool plug test tubes, add water to 1.0mL,
Precision adds 5% phenol solution 1mL (I is now prepared before use), shakes up, then accurate addition sulfuric acid 5mL, shakes up, puts in boiling water bath
Heating 20 minutes, takes out, and is put into ice-water bath and cools down 5 minutes, using corresponding reagent as blank, determines and inhales at 488nm wavelength
Luminosity, using absorbency Y as ordinate, concentration X (g/L) is abscissa, draws standard curve, and regression equation is：Y=11.772X-
0.1492, r=0.9995.As a result show that polysaccharide linear relationship in the range of 0.02-0.10g/L is good.
2.4 determination method
Precision measures need testing solution 1 and each 0.5mL of need testing solution 2 respectively, puts in 10mL tool plug test tubes, adds water to
1mL, by the method under the preparation of " 2.3 " standard curve, from " precision adds 5% phenol solution 1mL ", extinction being determined in accordance with the law
Degree, the content of DEXTROSE ANHYDROUS from need testing solution is read on standard curve, calculates, produces.
2.5 precision test
Precision draws 6 parts of glucose control product solution, and every part of 0.6mL determines absorbance according to the method under " 2.4 " item.
Measurement result, RSD is respectively 1.60% and 1.62%, and precision is good.
2.6 replica test
6 parts of need testing solutions 1 and need testing solution 2 are prepared as stated above, according to determining absorbance as stated above.Determine
As a result, RSD is respectively 2.70% and 2.71%, and repeatability is good.
2.7 stability test
Take same need testing solution 1 and need testing solution 2 respectively, place at room temperature, respectively at 10min, 30min, 1h,
Absorbance is determined after 2h, 4h, 6h as stated above.Measurement result, RSD is respectively 2.70% and 2.73%, shows that test sample is molten
Liquid 1 and need testing solution 2 are stable in 6 hours.
2.8 average recoveries are tested
6 parts of 1 powder of dendrobium candidum of known content (12.5%) is weighed, every part of about 1.65g is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares need testing solution 1, determines as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is that 94.9%, RSD is 2.7%.
6 parts of 2 powder of dendrobium candidum of known content (12.5%) is weighed, every part of about 1.65g is accurately weighed.It is accurate to add
Glucose control product solution (concentration is 0.099g/L) 2mL, prepares need testing solution 2, determines as stated above as stated above,
Polyoses content is calculated, as a result average recovery rate is that 93.9%, RSD is 2.6%.
2.9 samples are determined
By the preparation method and condition determination of above-mentioned need testing solution 1 and need testing solution 2,6 parts of samples of dendrobium candidum 1 are determined
The content of polysaccharide in product and 6 parts of samples of dendrobium candidum 2, records result, and results averaged the results are shown in Table 1.
The determination of polysaccharide result of table 1
As seen from table, the content of polysaccharide in the dendrobium candidum that the inventive method plantation is obtained, the dendrobium candidum finally harvested
It is higher.The quality better of dendrobium candidum.
Compared with prior art, during the inventive method plantation dendrobium candidum, the dendrobium candidum quality better finally plucked, effectively
Component content is high, particularly the height of the content of polysaccharide.
Embodiment
Embodiment 1.
A kind of implantation methods of dendrobium candidum, specifically include following steps：
(1) seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 75% ethanol disinfection is used on superclean bench
After 10min, then with aseptic water washing 3 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, be in daytime temperature
Under the conditions of 20-25 DEG C, 18-20 DEG C of night temperature, light culture, until seed is sprouted；The germination medium is by sucrose 5kg, agar
10kg, red sage root 15kg and tuber of dwarf lilyturf 15kg are made as follows：The red sage root and the tuber of dwarf lilyturf add the decocting of 11 times of amounts to boil after 2.5h, filter,
Filter residue is taken, coarse granule is dry, pulverize into, coarse granule is mixed with sucrose, agar, produced；
(2) seedling culture：After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, at 23-28 DEG C,
Daily illumination 9h under light intensity 1500-2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium by sucrose 5kg,
Sweet potato mash 15kg, soybean residue 10kg, ox Teng 10kg, bark of eucommia 15kg, asarum 10kg, levisticum 10kg, Radix Angelicae Sinensis 15kg and Chinese ephedra 15kg
It is made as follows：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter
Slag, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash and soybean residue, produced；
(3) seedling replanting：Transplanting time is mid-April, and height of seedling is 5-8cm dendrobium candidum seedling, first cleans children with clear water
The culture medium of seedling root, then the sterilization of immersion root is diluted with 0.4% potassium permanganate, it is 10-15cm dendrobium candidum seeds by line-spacing
In matrix, it is ensured that matrix covers root system, root system is set to be contacted with matrix；The matrix is by sucrose 5kg, sweet potato mash 15kg, soil
Beans mud 15kg, cornmeal mush 10kg, soybean residue 10kg, cultivated land 15kg, root bark of tree peony 10kg, kuh-seng 15kg, fruit of glossy privet 10kg and evodia rutaecarpa
15kg is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 11 times of amounts to boil after 2.5h, filter,
Filter residue is taken, coarse granule is dry, pulverize into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) manage：
A. water：Watered using the mode of spraying, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 1 3-6 months
It is secondary, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time；
B sunshades：Summer carries out sunshade using the sunshade net of 70-80% sunshade degree；
C. Temperature and Humidity Control：It is 60-70% to control air humidity, and temperature is 20-25 DEG C；
D. chemical fertilizer topdresses and applied in three times, for the first time 3 months after seedling replanting, and second after seedling replanting
4 months, for the third time 6 months after seedling replanting；The matrix is by sucrose 5kg, sweet potato mash 15kg, mashed potatoes 15kg, corn
Paste 10kg, soybean residue 10kg, cultivated land 15kg, root bark of tree peony 10kg, kuh-seng 15kg, fruit of glossy privet 10kg and evodia rutaecarpa 15kg as follows
It is made：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 11 times of amounts to boil after 2.5h, filter, take filter residue, dry, powder
Coarse granule is broken into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(5) March November to next year, harvesting, you can.
Embodiment 2.
A kind of implantation methods of dendrobium candidum, specifically include following steps：
(1) seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection is used on superclean bench
After 15min, then with aseptic water washing 4 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, be in daytime temperature
20-25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；The germination medium is by sucrose 6kg, agar
15kg, red sage root 20kg and tuber of dwarf lilyturf 20kg are made as follows：The red sage root and the tuber of dwarf lilyturf add the decocting of 12 times of amounts to boil after 3h, filter, take
Filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced；
(2) seedling culture：After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, at 23-28 DEG C,
Daily illumination 10h under light intensity 1500-2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium by sucrose 6kg,
Sweet potato mash 20kg, soybean residue 15kg, ox Teng 15kg, bark of eucommia 20kg, asarum 15kg, levisticum 15kg, Radix Angelicae Sinensis 20kg and Chinese ephedra 20kg
It is made as follows：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the decocting of 12 times of amounts to boil after 3h, filter, take filter
Slag, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced；
(3) seedling replanting：Transplanting time is the 4-6 months, and height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling with clear water
The culture medium of root, then diluted immersion root with 0.5% potassium permanganate and sterilized, by line-spacing be 10-15cm dendrobium candidums seed in
In matrix, it is ensured that matrix covers root system, root system is set to be contacted with matrix；The matrix is by sucrose 6kg, sweet potato mash 20kg, potato
Mud 20kg, cornmeal mush 15kg, soybean residue 15kg, cultivated land 20kg, root bark of tree peony 15kg, kuh-seng 20kg, fruit of glossy privet 15kg and evodia rutaecarpa
20kg is made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 12 times of amounts to boil after 3h, filter, take
Filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) manage：
A. water：Watered using the mode of spraying, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 1 3-6 months
It is secondary, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time；
B sunshades：Summer carries out sunshade using the sunshade net of 70-80% sunshade degree；
C. Temperature and Humidity Control：It is 60-70% to control air humidity, and temperature is 20-25 DEG C；
D. chemical fertilizer topdresses and applied in three times, for the first time 3 months after seedling replanting, and second after seedling replanting
5 months, for the third time 6 months after seedling replanting；
(5) March November to next year, harvesting, you can.
Embodiment 3.
A kind of implantation methods of dendrobium candidum, specifically include following steps：
(1) seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70% ethanol disinfection is used on superclean bench
After 5min, then with aseptic water washing 3 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, be in daytime temperature
20-25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；The germination medium is by sucrose 3kg, agar
5kg, red sage root 10kg and tuber of dwarf lilyturf 10kg are made as follows：The red sage root and the tuber of dwarf lilyturf add the decocting of 10 times of amounts to boil after 2h, filter, take filter
Slag, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced；
(2) seedling culture：After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, at 23-28 DEG C,
Daily illumination 9h under light intensity 1500-2500lx, until dendrobium candidum height of seedling is 5-8cm；The proliferated culture medium by sucrose 3kg,
Sweet potato mash 10kg, soybean residue 5kg, ox Teng 5kg, bark of eucommia 10kg, asarum 5kg, levisticum 5kg, Radix Angelicae Sinensis 10kg and Chinese ephedra 10kg are pressed
The method of stating is made：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the decocting of 10 times of amounts to boil after 2h, filter, take filter residue, do
It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, soybean residue, produced；
(3) seedling replanting：Transplanting time is the 4-6 months, and height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling with clear water
The culture medium of root, then diluted immersion root with 0.3% potassium permanganate and sterilized, by line-spacing be 10-15cm dendrobium candidums seed in
In matrix, it is ensured that matrix covers root system, root system is set to be contacted with matrix；The matrix is by sucrose 3kg, sweet potato mash 10kg, potato
Mud 10kg, cornmeal mush 5kg, soybean residue 5kg, cultivated land 10kg, root bark of tree peony 5kg, kuh-seng 10kg, fruit of glossy privet 5kg and evodia rutaecarpa 10kg are pressed
Following methods are made：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the decocting of 10 times of amounts to boil after 2h, filter, take filter residue, do
It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced；
(4) manage：
A. water：Watered using the mode of spraying, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 1 3-6 months
It is secondary, 5-6 days 6-9 months 1 time, 2-3 days 9-12 months 1 time；
B. summer carries out sunshade using the sunshade net of 70-80% sunshade degree；
C. Temperature and Humidity Control：It is 60-70% to control air humidity, and temperature is 20-25 DEG C；
D. chemical fertilizer topdresses and applied in three times, for the first time 2 months after seedling replanting, and second after seedling replanting
4 months, for the third time 6 months after seedling replanting；
(5) March November to next year, harvesting, you can.
Claims (9)
1. a kind of implantation methods of dendrobium candidum, it is characterised in that：Sprouted including A, seed, B, seedling culture, C, seedling replanting,
D, management.
2. the implantation methods of dendrobium candidum as claimed in claim 1, it is characterised in that：Specifically include following steps：
Seed is sprouted：Seeds of Dendrobium Candidum is taken, after cleaning, 70-80% ethanol disinfection 5-15min is used on superclean bench
Afterwards, then with aseptic water washing 3-4 times, it is placed in after being blotted on the filter paper of sterilizing, is inoculated on germination medium, is 20- in daytime temperature
25 DEG C, under the conditions of 18-20 DEG C of night temperature, light culture, until seed is sprouted；
Seedling culture：After after seed sprouting, the high single seedlings of 1-2cm are inoculated on proliferated culture medium, in 23-28 DEG C, light intensity
Daily illumination 9-10h under 1500-2500lx, until dendrobium candidum height of seedling is 5-8cm；
（3）Seedling replanting：Transplanting time is the 4-6 months, and height of seedling is 5-8cm dendrobium candidum seedling, first cleans seedling root with clear water
Culture medium, then dilute immersion root with 0.3-0.5% potassium permanganate and sterilize, by line-spacing be 10-15cm dendrobium candidums seed in
In matrix, it is ensured that matrix covers root system, root system is set to be contacted with matrix；
Management：
A. water：Watered using the mode of spraying, irrigation times are 2-3 days 1-3 months 1 time, 1-2 days 3-6 months 1 time, 6-9
5-6 days month 1 time, 2-3 days 9-12 months 1 time；
B. sunshade：Summer carries out sunshade using the sunshade net of 70-80% sunshade degree；
C. Temperature and Humidity Control：It is 60-70% to control air humidity, and temperature is 20-25 DEG C；
D. chemical fertilizer topdresses and applied in three times, for the first time 2-3 months after seedling replanting, second of 4- after seedling replanting
5 months, for the third time 6 months after seedling replanting；
（5）Harvesting：March November to next year, harvesting, you can.
3. the implantation methods of dendrobium candidum as claimed in claim 2, it is characterised in that：The step（1）In, it is described to sprout training
Base is supported mainly by 10-20 parts of 5-15 parts of 3-6 parts of sucrose, agar, the red sage root calculating by weight and tuber of dwarf lilyturf 10-20 part by following
Method is made：The red sage root and the tuber of dwarf lilyturf add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, dry, pulverize into coarse granule, slightly
Particle is mixed with sucrose, agar, is produced.
4. the implantation methods of dendrobium candidum as claimed in claim 3, it is characterised in that：The germination medium is main by by weight
15 parts of 5 parts of sucrose, 10 parts of agar, 15 parts of the red sage root and the tuber of dwarf lilyturf that amount part calculates are made as follows：The red sage root and the tuber of dwarf lilyturf add 11 times
The decocting of amount is boiled after 2.5h, filtering, takes filter residue, dry, pulverize into coarse granule, and coarse granule is mixed with sucrose, agar, produced.
5. the implantation methods of dendrobium candidum as claimed in claim 2, it is characterised in that：The step（2）In, the propagation training
Support base main by 3-6 parts of the sucrose calculated by weight, 10-20 parts of sweet potato mash, 5-15 parts of soybean residue, 5-15 parts of ox Teng, the bark of eucommia
10-20 parts of 10-20 parts, 5-15 parts of asarum, 5-15 parts of levisticum, 10-20 parts of Radix Angelicae Sinensis and Chinese ephedra are made as follows：Ox Teng, Du
Secondary, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, dry, pulverize into thick
Grain, coarse granule is mixed with sucrose, sweet potato mash, soybean residue, is produced.
6. the implantation methods of dendrobium candidum as claimed in claim 5, it is characterised in that：The proliferated culture medium is main by by weight
Measure part calculate 5 parts of sucrose, 15 parts of sweet potato mash, 10 parts of soybean residue, 10 parts of ox Teng, 15 parts of the bark of eucommia, 10 parts of asarum, 10 parts of levisticum,
15 parts of 15 parts of Radix Angelicae Sinensis and Chinese ephedra are made as follows：Ox Teng, the bark of eucommia, asarum, levisticum, Radix Angelicae Sinensis and Chinese ephedra add the decocting of 11 times of amounts
Boil after 2.5h, filter, take filter residue, dry, pulverize into coarse granule, coarse granule is mixed with sucrose, sweet potato mash and soybean residue, produced.
7. the implantation methods of the dendrobium candidum as described in claim asks 2, it is characterised in that：The step（3）In, the matrix
Mainly by 3-6 parts of the sucrose calculated by weight, 10-20 parts of sweet potato mash, 10-20 parts of mashed potatoes, 5-15 parts of cornmeal mush, soybean residue
10-20 parts of 5-15 parts, 10-20 parts of cultivated land, 5-15 parts of the root bark of tree peony, 10-20 parts of kuh-seng, 5-15 parts of the fruit of glossy privet and evodia rutaecarpa press following sides
Method is made：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add the 10-12 times of decocting measured to boil after 2-3h, filtering, take filter residue, do
It is dry, coarse granule is ground into, coarse granule is mixed with sucrose, sweet potato mash, mashed potatoes, cornmeal mush and soybean residue, produced.
8. the implantation methods of the dendrobium candidum as described in claim asks 7, it is characterised in that：The matrix is main by by weight
5 parts of the sucrose of calculating, 15 parts of sweet potato mash, 15 parts of mashed potatoes, 10 parts of cornmeal mush, 10 parts of soybean residue, 15 parts of cultivated land, 10 parts of the root bark of tree peony,
15 parts of 15 parts of kuh-seng, 10 parts of the fruit of glossy privet and evodia rutaecarpa are made as follows：Cultivated land, the root bark of tree peony, kuh-seng, the fruit of glossy privet and evodia rutaecarpa add
The 10-12 times of decocting measured is boiled after 2-3h, filtering, takes filter residue, dry, pulverize into coarse granule, coarse granule and sucrose, sweet potato mash, soil
Beans mud, cornmeal mush and soybean residue are mixed, and are produced.
9. the implantation methods of the dendrobium candidum as described in claim asks 2, it is characterised in that：The step（4）In, the chemical fertilizer
For nitrogenous fertilizer, calculated by N, amount of application is 138-207kg/hm2, apply fertilizer in two times, be for the first time seed manure, amount of application accounts for total N content
2/10, second is to topdress, and amount of application accounts for the 3/10 of total N content, for the third time to topdress, and amount of application accounts for the 5/10 of total N content.
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