CN106947006B - A kind of production method of high-purity inulin - Google Patents
A kind of production method of high-purity inulin Download PDFInfo
- Publication number
- CN106947006B CN106947006B CN201710276795.2A CN201710276795A CN106947006B CN 106947006 B CN106947006 B CN 106947006B CN 201710276795 A CN201710276795 A CN 201710276795A CN 106947006 B CN106947006 B CN 106947006B
- Authority
- CN
- China
- Prior art keywords
- inulin
- extracting solution
- obtains
- crude extract
- purity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920001202 Inulin Polymers 0.000 title claims abstract description 84
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 title claims abstract description 84
- 229940029339 inulin Drugs 0.000 title claims abstract description 84
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 238000001728 nano-filtration Methods 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims abstract description 16
- 239000000287 crude extract Substances 0.000 claims abstract description 14
- 244000298479 Cichorium intybus Species 0.000 claims abstract description 11
- 235000007542 Cichorium intybus Nutrition 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000000227 grinding Methods 0.000 claims abstract description 5
- 238000011033 desalting Methods 0.000 claims abstract description 4
- 239000000284 extract Substances 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 229920001661 Chitosan Polymers 0.000 claims description 20
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 19
- 239000002131 composite material Substances 0.000 claims description 9
- 239000000654 additive Substances 0.000 claims description 8
- 230000000996 additive effect Effects 0.000 claims description 8
- 229920002492 poly(sulfone) Polymers 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 125000002091 cationic group Chemical group 0.000 claims description 4
- 230000004907 flux Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 150000001768 cations Chemical class 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 abstract description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 6
- 239000005720 sucrose Substances 0.000 abstract description 6
- 150000002772 monosaccharides Chemical class 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 19
- 235000013305 food Nutrition 0.000 description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000011017 operating method Methods 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000019219 chocolate Nutrition 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000001223 reverse osmosis Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 241000723343 Cichorium Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 244000111146 Sonchus arvensis Species 0.000 description 1
- 235000008132 Sonchus arvensis ssp. uliginosus Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- BJHIKXHVCXFQLS-UYFOZJQFSA-N fructose group Chemical group OCC(=O)[C@@H](O)[C@H](O)[C@H](O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 description 1
- 235000021433 fructose syrup Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 235000011496 sports drink Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of production methods of high-purity inulin, and this method comprises the following steps: (1) grinding: witloof being cleaned, peeling, grinding crushing；(2) it extracts: the witloof of crushing is added in inulin extracting solution, 2~3h is stirred under 35~40 degrees Celsius, extracting solution is then heated to 100 DEG C, stir 5~8h；(3) clean: filtering inulin extracting solution obtains inulin crude extract；(5) it is decolourized to inulin crude extract, de- bitter, desalting processing, obtains inulin extracting solution A；(6) the inulin extracting solution A obtained with nanofiltration membrane treatment step (5) collects filter liquor, obtains inulin solution, freeze-dried to obtain high-purity inulin.The inulin property that the present invention obtains is stablized, purity is high, and monosaccharide, the content of sucrose are few in product, and wherein the content of inulin is 95% or more.
Description
Technical field
The invention belongs to inulin production technical fields, and in particular to a kind of production method of high-purity inulin.
Background technique
Witloof is composite family Cichorium herbaceos perennial, also known as European witloof, coffee grass or French sowthistle, is originated in
Europe, West Asia, the Central Asia and North America, the area such as northwest China, North China and northeast are distributed.Chrysanthemum fragrant plant nutritive value is high, disease-resistant
Insect pest and high yield, power of regeneration is strong, is a kind of high yield and high quality forage grass for having very much potentiality to be exploited.Witloof fleshy root includes 70% or so
The inulin of (dry matter weight) can be used as the raw materials for production of inulin, oligofructose (FOS) and high fructose syrup.
Inulin (inulin) is also known as synanthrin, is that the chain as made of β-D-Fructose glycosidic bond (1 → 2) connection multiple fructosyls is more
An alpha-D-glucose base is often contained in sugar, end.The molecular formula of inulin is expressed as GFn or Fm.Wherein G is terminal glucose
Unit (Glucose), F are represented fructose molecule (Fructose), and n or m then represent fructose units number.In general, a glycosidic bond
One degree of polymerization (DP), inulin (DP=2~9) in low polymerization degree is called oligofructose, when only one fructosyl and grape
Glycosyl be connected when both DP=1 when be exactly sucrose (sucrose).In fact, sucrose is not belonging to inulin.
Inulin can extend the energy supply time of carbohydrate in human body and not significantly improve blood glucose level, metabolic process
Insulin participation is not needed, helps to reduce diabetes patient to the dependence and needs of insulin, controls blood glucose level.To enteron aisle
The growth of the beneficial bacteriums such as Bifidobacterium, Bacillus acidi lactici also has apparent facilitation.
Inulin is a kind of natural component being present in various vegetables and fruit, is classified as naturally in many American-European countries
Food ingredient.In recent years, great attention of the development and utilization of inulin by international food circle, and it is successfully applied in dairy products, face
The field of food such as packet, candy, beverage and seasoning.In China, attention with people to health, the development and utilization of inulin also by
Gradually get up extensively.Inulin can substitute sucrose use in chocolate, not need other adjusting process, manufactured chocolate
It is heat-resisting and be rich in fiber, it is in good taste.Inulin is used as sweetener, will not expand the change of tooth dental caries.Inulin is in cereal foods or swollen
It is used in helping digestion product, the brittleness and dilatancy of food can be increased.Inulin can make water form the same mouthfeel of grease, therefore alternative
Grease is for producing the products such as meat products, cream, the leaching of ice fine jade.Inulin can be milk, Yoghourt, dairy beverage and bean product etc.
The mouthfeel of low fat food offer full fat product sample.In tablespread, inulin has melting similar with high-fat product and stream
Denaturation, can stable emulsion in favor of smear.Inulin as a kind of food fibres have neutral taste, can cover bitterness and to
The feeling of people's softness, suitable for being used in the sports drink of high microsteping low in calories.
Nanofiltration be the mid-80 grow up belong to pressure-actuated novel membrane separation technique, nanofiltration membrane can retain point
Son amount be about 200~1000Da, between ultrafiltration and it is reverse osmosis between, filled up ultrafiltration and it is reverse osmosis between blank.Using receiving
The separation to high relative molecular mass organic matter and relative molecular mass organic matter may be implemented in filter membrane.In the prior art
Have been reported that the report using nanofiltration membrane purification oligofructose, but nanofiltration membrane is not easy to be high by material pollution, recycling rate of waterused, leads
The production cost increases for cause.
Summary of the invention
The technical problem to be solved in the invention is to provide a kind of production method for preparing high-purity inulin, existing to solve
There is the problem that inulin purity is not high in technology.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of production method of high-purity inulin, this method comprises the following steps:
(1) it grinds: witloof being cleaned, peeling, grinding crushing；
(2) it extracts: the witloof of crushing is added in inulin extracting solution, 2~3h is stirred under 35~40 degrees Celsius, then will
Extracting solution is heated to 100 DEG C, stirs 5~8h；
(3) clean: filtering inulin extracting solution obtains inulin crude extract；
(5) it is decolourized to inulin crude extract, de- bitter, desalting processing, obtains inulin extracting solution A；
(6) the inulin extracting solution A obtained with nanofiltration membrane treatment step (5) collects filter liquor, inulin solution is obtained, through cold
Jelly is dried to obtain high-purity inulin.
In step (2), the inulin extracting solution is the Tris-HCl buffer containing pectase, and the Tris-HCl is slow
The concentration of fliud flushing is 1mol/L, pH 6.8.
Wherein, the additive amount of pectase is 300~1000U/L
It is described to be decolourized to inulin crude extract, take off suffering reason in step (5), it is that inulin crude extract is passed sequentially through into shell
Glycan and diatom earth mixtures and cationic macroreticular resin.
Wherein, the mixture of the chitosan and diatomite, wherein the mass ratio of chitosan and diatomite is 2~3:1.
Wherein, the cationic macroreticular resin is XDA-5 or D941.
In step (6), the nanofiltration membrane is the sulfonated polysulfone composite nanometer filtering film that aperture is 0.2~0.6 nanometer.
Wherein, the sulfonated polysulfone composite nanometer filter film process inulin extracting solution for being 0.2~0.6 nanometer using aperture, processing
Condition is 200~400L/m of flux3H, 0.8~1MPa of pressure, pH6.8.
Wherein, the sulfonated polysulfone composite nanometer filtering film is NTR-7410.
The utility model has the advantages that
The invention discloses a kind of production method of high-purity inulin, obtained inulin property is stablized, purity is high, in product
Monosaccharide, the content of sucrose are few, and wherein the content of inulin is 95% or more.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies content described in example and is merely to illustrate the present invention, without sheet described in detail in claims should will not be limited
Invention.
Embodiment 1:
Take the net fresh witloof of 500g, peeling, grinding crushing；The Tris-HCl containing pectase is added in the witloof of crushing to delay
In fliud flushing, the concentration of the Tris-HCl buffer is 1mol/L, and pH 6.8, the additive amount of pectase is 300~1000U/L,
2~3h is stirred under 35~40 degrees Celsius, extracting solution is then heated to 100 DEG C, stirs 5~8h；Inulin extracting solution is filtered, is obtained
To inulin crude extract；
It is decolourized to inulin crude extract, de- bitter, desalting processing, inulin crude extract is passed sequentially through into chitosan and diatomite
The mass ratio of mixture and cationic macroreticular resin, chitosan and diatomite is 2~3:1, and the cation macroreticular resin is
XDA-5 or D941 obtains inulin extracting solution A；
With nanofiltration membrane inulin extracting solution A, nanofiltration membrane is the sulfonated polysulfone composite nanometer filtering film that aperture is 0.2~0.6 nanometer,
Treatment conditions are 200~400L/m of flux3H, 0.8~1MPa of pressure, pH6.8 collect filter liquor, obtain inulin solution, institute
The sulfonated polysulfone composite nanometer filtering film stated is NTR-7410, freeze-dried to obtain high-purity inulin.
Embodiment 2: influence of the additive amount of pectase to inulin extraction efficiency.
Operating method with embodiment 1, except that the additive amount for adjusting pectase is 300U/L, 500U/L, 700U/L,
900U/L、1000U/L
Influence of the additive amount of 1 glue enzyme of table to inulin extraction efficiency
| Pectase additive amount | 300U/L | 500U/L | 700U/L | 900U/L | 1000U/L |
| Inulin yield | 68% | 75% | 78% | 85% | 88% |
Embodiment 3: influence of the pectin enzyme action time to inulin yield.
Operating method is 900U/L using the additive amount of pectase with embodiment 1, and the processing time for adjusting pectase is
1h、2h、3h、4h、5h。
Influence of the 2 pectin enzyme action time of table to inulin yield
| Time | 1h | 2h | 3h | 4h | 5h |
| Inulin yield | 55% | 65% | 85% | 84% | 83% |
Embodiment 4: the mixture of chitosan and diatomite decolourizes to inulin solution to take off the influence of bitter efficiency.
The inulin crude extract with the mass ratio of chitosan and diatomite is respectively 1:1, shell with embodiment 1 by operating method
The mass ratio of glycan and diatomite be the mass ratio of 2:1, chitosan and diatomite be 3:1, the mass ratio of chitosan and diatomite
Mass ratio for 4:1, chitosan and diatomite is that 5:1 carries out suffering reason of decolourizing.
Then decolorizing effect is determined by the absorptance of the oligofructose mixed liquor at detection 560nm.The meter of percent of decolourization
Shown in calculation method formula (1):
It the results are shown in Table 3 and table 4.
The influence of 3 chitosan of table and diatomite to inulin solution decolorizing efficiency
| Light transmittance % | Percent of decolourization % | |
| Chitosan | 63 | 60 |
| Chitosan: diatomite=1:1 | 71 | 78 |
| Chitosan: diatomite=2:1 | 90 | 84 |
| Chitosan: diatomite=3:1 | 96 | 87 |
| Chitosan: diatomite=4:1 | 85 | 76 |
| Chitosan: diatomite=5:1 | 84 | 75 |
The influence of 4 chitosan of table and diatomite bitter efficiency de- to inulin solution
Embodiment 5: after nanofiltration membrane treatment in extracting solution inulin content.
Nanofiltration processing is carried out to oligofructose solution with NTR-7410 nanofiltration membrane, after nanofiltration in solution various sugars content
As shown in table 7.The condition used when nanofiltration are as follows:
Result after 7 NTR-7410 nanofiltration membrane treatment of table
| Monosaccharide (%) | Inulin (%) | |
| Before nanofiltration | 5 | 95 |
| After nanofiltration | 0 | 99 |
As can be seen from Table 7, NTR-7410 nanofiltration membrane has preferable nanofiltration effect to oligofructose mixed solution, obtains
The oligofructose content arrived is 99% or more.
Claims (1)
1. a kind of production method of high-purity inulin, which is characterized in that this method comprises the following steps:
(1) it grinds: witloof being cleaned, peeling, grinding crushing；
(2) it extracts: the witloof of crushing is added in inulin extracting solution, 2~3h is stirred under 35~40 degrees Celsius, then will extract
Liquid is heated to 100 DEG C, stirs 5~8h；
(3) clean: filtering inulin extracting solution obtains inulin crude extract；
(5) it is decolourized to inulin crude extract, de- bitter, desalting processing, obtains inulin extracting solution A；
(6) the inulin extracting solution A obtained with nanofiltration membrane treatment step (5) collects filter liquor, obtains inulin solution, chilled dry
It is dry to obtain high-purity inulin；
In step (2), the inulin extracting solution is the Tris-HC1 buffer containing pectase, the Tris-HC1 buffer
Concentration be lmol/L, pH 6.8；
The additive amount of pectase is 300 ~ 1000U/L；
It is described to be decolourized to inulin crude extract, take off suffering reason in step (5), it is that inulin crude extract is passed sequentially through into chitosan
With diatom earth mixtures and cationic macroreticular resin；
The mixture of the chitosan and diatomite, wherein the mass ratio of chitosan and diatomite is 2~3:1；
The cation macroreticular resin is XDA-5 or D941；
In step (6), the nanofiltration membrane is the sulfonated polysulfone composite nanometer filtering film that aperture is 0.2~0.6 nanometer；
The sulfonated polysulfone composite nanometer filter film process inulin extracting solution for being 0.2 ~ 0.6 nanometer using aperture, treatment conditions are flux
200~400L/m2H, 0.8 ~ 1MPa of pressure, pH6.8；
The sulfonated polysulfone composite nanometer filtering film is NTR-7410.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710276795.2A CN106947006B (en) | 2017-04-25 | 2017-04-25 | A kind of production method of high-purity inulin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710276795.2A CN106947006B (en) | 2017-04-25 | 2017-04-25 | A kind of production method of high-purity inulin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106947006A CN106947006A (en) | 2017-07-14 |
| CN106947006B true CN106947006B (en) | 2019-05-14 |
Family
ID=59477440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710276795.2A Active CN106947006B (en) | 2017-04-25 | 2017-04-25 | A kind of production method of high-purity inulin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106947006B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165592B (en) * | 2017-11-27 | 2020-08-07 | 深圳市华利康纤生物科技有限公司 | Method for extracting inulin by using mesoporous material immobilized complex enzyme |
| CN110066348A (en) * | 2018-01-23 | 2019-07-30 | 天津科技大学 | A method of it extracts, purification witloof oligosaccharide |
| US20220177607A1 (en) | 2019-03-08 | 2022-06-09 | Sensus B.V. | Inulin composition and method of purifying inulin |
| CN110301645A (en) * | 2019-06-18 | 2019-10-08 | 江苏碧青园海洋生物科技有限公司 | A kind of synanthrin composition and preparation method |
| CN112552427B (en) * | 2020-07-01 | 2024-06-04 | 吉林省石油化工设计研究院 | Molecular-grade production process and device of inulin |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706957A (en) * | 2005-04-08 | 2005-12-14 | 江苏天士力帝益药业有限公司 | Process of extracting synanthrin from girasole |
| CN101402984A (en) * | 2008-11-12 | 2009-04-08 | 南京泛成生物化工有限公司 | Method for extracting inulin with assistance of biological enzyme |
| CN101798354A (en) * | 2009-05-22 | 2010-08-11 | 上海博程生物科技有限公司 | Method for coproducing jerusalem artichoke inulin and jerusalem artichoke insoluble diedairy fiber |
| CN104292367A (en) * | 2014-10-15 | 2015-01-21 | 重庆骄王天然产物股份有限公司 | Refining method of inulin |
| CN106349398A (en) * | 2015-11-13 | 2017-01-25 | 翟丹云 | Cichorium intybus inulin extracting method |
-
2017
- 2017-04-25 CN CN201710276795.2A patent/CN106947006B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706957A (en) * | 2005-04-08 | 2005-12-14 | 江苏天士力帝益药业有限公司 | Process of extracting synanthrin from girasole |
| CN101402984A (en) * | 2008-11-12 | 2009-04-08 | 南京泛成生物化工有限公司 | Method for extracting inulin with assistance of biological enzyme |
| CN101798354A (en) * | 2009-05-22 | 2010-08-11 | 上海博程生物科技有限公司 | Method for coproducing jerusalem artichoke inulin and jerusalem artichoke insoluble diedairy fiber |
| CN104292367A (en) * | 2014-10-15 | 2015-01-21 | 重庆骄王天然产物股份有限公司 | Refining method of inulin |
| CN106349398A (en) * | 2015-11-13 | 2017-01-25 | 翟丹云 | Cichorium intybus inulin extracting method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106947006A (en) | 2017-07-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106947006B (en) | A kind of production method of high-purity inulin | |
| US5403599A (en) | Method for preparing tamarind oligosaccharides | |
| CN106343547A (en) | Preparation method for plant/fruit/vegetable enzymes | |
| CN103385515B (en) | Plant drink and preparation method thereof | |
| CN107325205B (en) | A kind of inulin and oligofructose syrup co-production | |
| CN104996970A (en) | Compound crystalline sweetener and preparation method thereof | |
| CN102860548A (en) | Jerusalem artichoke juice and active lactobacillus beverage and preparation method thereof | |
| CN101113404A (en) | Preparation method of apple vinegar | |
| TWI729232B (en) | Extracts from fruits of the cucurbitaceae family, and methods of preparing thereof | |
| CN109247467A (en) | A kind of pure taste inspissated juice of no albumen Siraitia grosvenorii and its production method | |
| CN101273782B (en) | Edible antifreezing fibre fruit and method for preparing the same | |
| CN104171795A (en) | Preparation method of natural rose and purple potato syrup | |
| CN115968991A (en) | Preparation method of dendrobium officinale suspension beverage | |
| CN103380927A (en) | Shine skin papaya juice beverage and preparation method thereof | |
| CN101130742A (en) | Preparation method of grape vinegar | |
| CN104531484B (en) | A kind of Bulbus Allii juice, the preparation method of applejack bland | |
| CN104286178A (en) | Compound fermented beverage of great burdock oligosaccharide strawberry yogurt and manufacturing method of compound fermented beverage | |
| CN106947000B (en) | A kind of preparation method of high-purity polyfructosan | |
| CN101113403A (en) | Preparation method of cantaloupe vinegar | |
| CN104522788B (en) | A kind of Bulbus Allii juice, the preparation method of apple vinegar bland | |
| KR20190078716A (en) | Manufacturing Method of Cooking grain syrup | |
| JP3515078B2 (en) | Taste improvement of food and drink by coffee oligosaccharide | |
| CN111264846A (en) | Chinese wolfberry extract sweet potato vermicelli and preparation method thereof | |
| CN104082410B (en) | A kind of hairy grape collagen polypeptide Ca yogurt | |
| CN102860561B (en) | A kind of sour-sweet type soft drink composite main agent and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 810000 Qinghai Xining biological science and Technology Industrial Park, No. four road 10. Applicant after: Van Neng special sugar industry Co., Ltd. Address before: 810000 Qinghai Xining biological science and Technology Industrial Park, No. four road 10. Applicant before: Dragon special Sugar Co. |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200210 Address after: 518000 floor 28-03, Weisheng technology building, No. 9966, Shennan Avenue, Maling community, Yuehai street, Nanshan District, Shenzhen City, Guangdong Province Patentee after: Shenzhen hualikang fiber Biotechnology Co., Ltd Address before: 810000 Xining biological science and Technology Industrial Park, No. four, No. 10, Qinghai Patentee before: Van Neng special sugar industry Co., Ltd. |
|
| TR01 | Transfer of patent right | ||
| CP03 | Change of name, title or address |
Address after: 100000-101-086, No. 5, Jianguomen North Street, Dongcheng District, Beijing Patentee after: Beijing hualikang fiber Biotechnology Co.,Ltd. Address before: 518000 floor 28-03, Weisheng technology building, 9966 Shennan Avenue, Maling community, Yuehai street, Nanshan District, Shenzhen City, Guangdong Province Patentee before: Shenzhen hualikang fiber Biotechnology Co.,Ltd. |
|
| CP03 | Change of name, title or address |