CN106914289B - Fabrication device and method of microfluidic chip - Google Patents

Abstract

The invention relates to a manufacturing device and a manufacturing method of a microfluidic chip. The manufacturing device of the microfluidic chip includes a microfluidic chip forming mold, a box body, a box cover, a puncher and a support sheet, and the surface of the microfluidic chip forming mold is provided with a forming hole communicating with a preset flow channel; The box body has a molding cavity, and the bottom of the box body is used to fixedly place the microfluidic chip molding mold; the box cover can be buckled and connected with the box body, and the box cover is provided with a cover; the puncher is suitable for the forming hole Matching, the puncher can be inserted into the forming hole through the cover opening; the support piece is detachably arranged on the box cover, and is used to support the puncher inserted in the forming hole. The microfluidic chip manufacturing device has low hole roughness, high uniformity of the transverse diameter of the hole, and less dross in the processing process, which has little influence on the change of the fluid parameter of the chip channel, and has high accuracy and precision of sample analysis.

Description

Fabrication device and method of microfluidic chip

technical field

The invention relates to the field of microfluidic chips, in particular to a manufacturing device and a manufacturing method of a microfluidic chip.

Background technique

The microfluidic chip integrates the basic operation units such as sample preparation, reaction, separation, and detection into a micron-scale chip, and automatically completes the entire analysis process. It has been widely used in biology, chemistry, medicine and other research fields. The processing methods of microfluidic chips mainly adopt hot pressing method, injection molding method or transverse plastic molding method. The material of the chip mainly uses silicon material, glass quartz or organic polymer, among which polydimethylsiloxane (PDMS) in the organic polymer has good light transmittance, biocompatibility, easy processing and low production cost, etc. Advantages are widely applied.

In the process of microfluidic chip processing, in the traditional technology, holes such as sample injection hole, sample sample outlet hole, waste liquid injection hole and waste liquid outlet hole are made by manual punching with a punching needle. However, due to the smoothness of the punch needle and the material properties of PDMS, the edge roughness of the processed hole is high, and the transverse diameter of the hole channel is inconsistent, which will cause adsorption or leakage of the sample during use. In addition, the dross generated during the punching process of the tine will cause changes in the fluid parameters of the chip channel, and even block the chip, which will affect the accuracy and precision of sample analysis.

Contents of the invention

Based on this, it is necessary to provide a manufacturing device and a manufacturing method of a microfluidic chip with low roughness, high uniformity of the lateral diameter of the channel, and less dross during processing.

A fabrication device for a microfluidic chip comprises:

A microfluidic chip forming mold, the surface of the microfluidic chip forming mold is provided with a forming hole communicating with a preset flow channel;

A box body, the box body has a molding cavity, and the bottom of the box body is used to fixedly place the microfluidic chip molding mold;

a box cover, the box cover can be buckled and connected with the box body, and the box cover is provided with a cover opening;

a hole punch adapted to the profiled hole, the hole punch being insertable through the cover opening into the profiled hole; and

A supporting piece, the supporting piece is detachably arranged on the box cover, and is used for supporting the puncher inserted into the forming hole.

The manufacturing device of the microfluidic chip includes a microfluidic chip forming mold, a box body, a box cover, a puncher and a support sheet, the microfluidic chip forming mold is fixedly placed on the bottom of the box body, and the punching The hole punch is adapted to the forming hole in the microfluidic chip forming mold, and the hole punch can be inserted into the forming hole through the cover opening. Through the mutual cooperation of the microfluidic chip forming mold, the box body, the box cover, the puncher and the support sheet, the manufacturing device of the microfluidic chip can directly complete the sample injection hole and the sample output during the microfluidic chip molding process. Compared with the traditional manual drilling, the holes required for the sample hole, waste liquid injection hole and waste liquid outlet hole are produced with low roughness, high uniformity of the transverse diameter of the channel, and dross during processing It has little influence on the change of fluid parameters in the chip channel, and the accuracy and precision of sample analysis are high. In addition, the manufacturing device of the microfluidic chip has a simple structure, which is convenient for the industrialized production of the microfluidic chip.

In one embodiment, the diameter of the box is 1cm-1m, and the material of the box is one or both of polypropylene and polystyrene. By setting the diameter of the box body to 1cm-1m, it can be used to fix microfluidic chip molding molds in a relatively large size range. The box body is made of one or two transparent materials of polypropylene and polystyrene, which is convenient for observing the molding process in the box body, and is also easy for the processing and production of the box body.

In one embodiment, an adhesive layer is further provided between the bottom of the box body and the microfluidic chip forming mold, and the adhesive layer is used to fix the microfluidic chip forming mold.

In one embodiment, the material of the bonding layer is polydimethylsiloxane. Polydimethylsiloxane is a transparent bonding material, which is convenient for observing the molding process inside the box.

In one of the embodiments, a height scale line is provided on the side wall of the box body. By providing a height scale line, the addition amount of the prepolymer can be better adjusted, and thus the thickness of the cast microfluidic chip can be precisely controlled.

In one embodiment, the diameter of the box cover is 1cm-1m, and the material of the box cover is one or both of polypropylene and polystyrene.

In one embodiment, the box cover is fastened to the box body through screw connection. Through screw connection, it is convenient to assemble and install the box body and the box cover.

In one of the embodiments, it further includes a three-dimensional adjustment frame, the three-dimensional adjustment frame is connected to the hole puncher, and is used for fine-tuning the position where the hole puncher is inserted into the forming hole. With a three-dimensional adjustment frame, the punch can be fine-tuned in all directions to ensure that the punch is accurately inserted into the forming hole and the hole manufacturing accuracy is ensured.

In one embodiment, the hole punch has a diameter of 1 μm-100 mm.

In addition, it is also necessary to provide a method for manufacturing a microfluidic chip.

A method for manufacturing a microfluidic chip, which is manufactured using the device for manufacturing a microfluidic chip described in any of the above-mentioned embodiments, and the manufacturing method specifically includes the following steps:

Fixing the forming mold of the microfluidic chip on the bottom of the box;

Fasten and connect the box cover with the box body;

Inserting the hole puncher into the forming hole of the microfluidic chip forming mold through the cover opening;

The support sheet is arranged on the box cover and supports and fixes the puncher;

Adding the prepolymer into the molding cavity of the box body through the cover opening;

Heat and dry the box with the prepolymer, and cool to room temperature after heating;

After the support sheet is disassembled from the box cover, the puncher is pulled out vertically to complete the fabrication of the holes in the microfluidic chip.

The microfluidic chip produced by the microfluidic chip manufacturing method has low hole roughness, high uniformity of the transverse diameter of the hole, and less dross in the processing process, which has little influence on the change of the fluid parameter of the chip channel, and the accuracy of sample analysis is high. High precision. In addition, the manufacturing method of the microfluidic chip is simple and easy to operate, and is convenient for industrial production.

Description of drawings

FIG. 1 is a schematic diagram of a box structure of a manufacturing device for a microfluidic chip according to an embodiment;

FIG. 2 is a schematic diagram of the structure of the box cover in the manufacturing device of the microfluidic chip shown in FIG. 1 .

The reference signs are explained as follows:

10. Microfluidic chip production device, 100. Microfluidic chip forming mold, 101. Forming hole, 110. Box body, 120. Box cover, 121. Cover opening, 130. Puncher, 140. Support sheet.

Detailed ways

In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

It should be noted that when an element is referred to as being “fixed” to another element, it can be directly on the other element or there can also be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or intervening elements may also be present.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention.

In the embodiment shown in FIG. 1 and FIG. 2 , the microfluidic chip manufacturing device 10 includes a microfluidic chip forming mold 100 , a box body 110 , a box cover 120 , a hole punch 130 and a supporting sheet 140 .

The surface of the microfluidic chip forming mold 100 is provided with a forming hole 101 communicating with a preset flow channel. Optionally, the microfluidic chip forming mold 100 may be provided with a plurality of forming holes 101 . The forming hole 101 can be used to process various required holes such as sample inlet hole, sample outlet hole, waste liquid sample hole and waste liquid outlet hole in the microfluidic chip.

The box body 110 has a molding cavity, and the bottom of the box body 110 is used to place the microfluidic chip molding mold 100 fixedly. The size of the box body 110 is adapted to the size of the microfluidic chip forming mold 100 , and optionally, the size of the box body 110 is larger than the size of the microfluidic chip forming mold 100 .

In one embodiment, the diameter of the box body 110 is 1 cm-1 m, and the material of the box body 110 is one or two transparent materials such as polypropylene and polystyrene. By setting the diameter of the box body 110 to 1 cm-1 m, the box body 110 can be adapted to fix the microfluidic chip molding mold 100 of a wide range of sizes. The box body 110 is made of one or more transparent materials such as polypropylene and polystyrene, which is convenient for observing the molding process in the box body 110 and is also easy for the processing and production of the box body 110 .

In one embodiment, an adhesive layer (not shown) is provided between the bottom of the box body 110 and the microfluidic chip forming mold 100, and the adhesive layer is used to fix the microfluidic chip forming mold 100. , to ensure the molding precision of the microfluidic chip.

In one embodiment, the adhesive layer is made of one or more transparent materials such as polydimethylsiloxane, polypropylene and polystyrene. A transparent material such as polydimethylsiloxane, polypropylene or polystyrene is coated on the bottom of the box body 110 to fix the microfluidic chip forming mold 100 in the box body 110 .

In one embodiment, the side wall of the box body 110 is provided with a height scale line (not shown). By providing a height scale line, the addition amount of the prepolymer can be better adjusted, and thus the thickness of the cast microfluidic chip can be precisely controlled. The range of the height scale line can be specifically adjusted according to the height of the box body 110 .

The box cover 120 can be fastened and connected with the box body 110 . The size of the box cover 120 matches the size of the box body 110 to ensure that the box cover 120 will not move after being fastened and connected with the box body 110 . The box cover 120 is fastened to the box body 110 through size matching connections. Optionally, the box cover 120 is fastened to the box body 110 through screw connection. The assembly and installation of the box body 110 and the box cover 120 is facilitated by screw connection. The box cover 120 has a cover opening 121 . The hole punch 130 can move in the cover opening 121 to ensure that the hole punch 130 is inserted into the forming hole 101 accurately. The prepolymer liquid is also used to cast the microfluidic chip forming mold 100 through the cover port 121 . The specific size of the cover opening 121 is adjusted according to the position of the forming hole 101 in the microfluidic chip forming mold 100 and the casting amount of the prepolymer liquid, so as to ensure the movement of the punch 130 and facilitate casting of the prepolymer liquid.

In one embodiment, the diameter of the box cover 120 is 1 cm-1 m, and the box cover 120 is made of one or two transparent materials such as polypropylene and polystyrene. By setting the diameter of the box cover 120 to 1 cm-1 m, the box cover 120 can be suitable for casting microfluidic chip forming molds 100 of a wide range of sizes. The box cover 120 is made of one or more transparent materials such as polypropylene and polystyrene, which is convenient for controlling the molding process in the box body 110 and is also easy for the processing and production of the box cover 120 .

The hole punch 130 is adapted to the forming hole 101 , and the hole punch 130 can be inserted into the forming hole 101 through the cover opening 121 . The hole punch 130 can be made of metal such as steel, or hard plastic, as long as the dimensional accuracy of the holes can be guaranteed. When there are multiple forming holes 101 , there are correspondingly multiple hole punchers 130 . Optionally, the diameter of the hole punch 130 is 1 μm-100 mm. Further optionally, the hole punch 130 is a cylindrical structure.

The support piece 140 is detachably disposed on the box cover 120 for supporting the hole punch 130 inserted into the forming hole 101 . When the puncher 130 is inserted into the forming hole 101, the support sheet 140 is installed on the lid to support the puncher 130; when the puncher needs to be drawn out from the forming hole 101, the support Sheet 140 is removed from lid 120 . The support piece 140 can be made of metal material or hard plastic, as long as it can support the puncher 130 . The support piece 140 can support the puncher 130 through size matching or screw connection. When there are multiple punchers 130 , there are correspondingly multiple support sheets 140 .

In one embodiment, a three-dimensional adjustment frame (not shown in the figure) is also included, and the three-dimensional adjustment frame is connected to the puncher 130 for fine-tuning the position where the hole puncher 130 is inserted into the forming hole. Optionally, the three-dimensional adjustment frame is detachably arranged on the box cover 120 . With the three-dimensional adjustment frame, the hole punch 130 can be fine-tuned in all directions to ensure that the hole punch 130 is aligned with the forming hole 101 of the microfluidic chip forming mold 100 , and the manufacturing accuracy of the hole is ensured.

The microfluidic chip manufacturing device 10 includes a microfluidic chip forming mold 100, a box body 110, a box cover 120, a puncher 130 and a support sheet 140, and the microfluidic chip forming mold 100 is fixedly placed on the box body 110. The hole punch 130 is adapted to the forming hole 101 of the microfluidic chip forming mold 100 , and the hole punch 130 can be inserted into the forming hole 101 through the cover opening 121 . Through the mutual cooperation of the microfluidic chip forming mold 100, the box body 110, the box cover 120, the puncher 130, and the support sheet 140, the microfluidic chip manufacturing device 10 can directly complete the sample during the microfluidic chip molding process. Compared with the traditional manual punching, the hole roughness is low and the transverse diameter of the channel is high in consistency , and there is less dross in the processing process, which has little influence on the change of fluid parameters in the chip channel, and the accuracy and precision of sample analysis are high. In addition, the microfluidic chip manufacturing device 10 has a simple structure, which facilitates the industrial production of microfluidic chips.

In addition, it is also necessary to provide a method for manufacturing a microfluidic chip.

A method for manufacturing a microfluidic chip, which is manufactured using the microfluidic chip manufacturing device 10 of any of the above-mentioned embodiments, and the manufacturing method specifically includes the following steps:

The microfluidic chip forming mold 100 is fixedly placed on the bottom of the box body 110 . Optionally, the microfluidic chip forming mold 100 and the bottom of the box body 110 are bonded and fixed through an adhesive layer containing materials such as polydimethylsiloxane, polypropylene or polystyrene.

The box cover 120 is buckled and connected with the box body 110 , and the size of the box cover 120 matches the size of the box body 110 to ensure that the box cover 120 will not move after being buckled and connected with the box body 110 .

The hole punch 130 is inserted into the forming hole 101 of the microfluidic chip forming mold 100 through the cover opening 121 . The hole punch 130 is adapted to the forming hole 101 , and the hole punch 130 is adjusted to align with the forming hole 101 .

The support piece 140 is installed on the box cover 120 and supports and fixes the hole punch 130 . It is ensured that the hole punch 130 fixed in the forming hole 101 will not move during the process of adding the prepolymer liquid. The support piece 140 can support the puncher 130 through size matching or screw connection.

The prepolymer liquid is added into the molding cavity of the box body 110 through the cover opening 121 for casting the microfluidic chip molding mold 100 . Optionally, the prepolymer is the polymer polydimethylsiloxane (PDMS).

The box body 110 added with the prepolymer liquid is heated and dried, and cooled to room temperature after heating. Optionally, the box body 110 is heated in an oven at 65° C. for 4 hours.

After the supporting sheet 140 is disassembled from the box cover 120, the puncher 130 is pulled out vertically, and the hole forming on the microfluidic chip is completed. The follow-up processing procedure of the microfluidic chip will continue, and the production of the microfluidic chip can be completed.

The microfluidic chip manufactured by the microfluidic chip manufacturing method has low pore roughness, high uniformity of the transverse diameter of the pore channel, and less dross in the processing process, which has little influence on the change of the fluid parameter of the chip channel and accurate sample analysis. High accuracy and accuracy. In addition, the manufacturing method of the microfluidic chip is simple and easy to operate, and is convenient for industrial production.

The various technical features of the above-mentioned embodiments can be combined arbitrarily. To make the description concise, all possible combinations of the various technical features in the above-mentioned embodiments are not described. However, as long as there is no contradiction in the combination of these technical features, should be considered as within the scope of this specification.

The above-mentioned embodiments only express several implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but should not be construed as limiting the patent scope of the invention. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (9)

1. a kind of producing device of micro-fluidic chip characterized by comprising

Micro-fluidic chip molding die, the micro-fluidic chip molding die surface are equipped with the molding hole being connected to default runner；

Box body, the box body have forming cavity, and the bottom of the box body is for micro-fluidic chip molding die described in fixed placement；

Box cover, the box cover, which is connected through a screw thread, is fastened on the box body, and the box cover is equipped with lid mouth；

Punch, the punch are adapted with the molding hole, and the punch can be inserted into described by the lid mouth In molding hole；

Support chip, the support chip are detachably arranged on the box cover, be used to support be inserted in it is described in the molding hole Punch；And

Three-dimensional trim holder, the three-dimensional trim holder connect the punch, are inserted into the molding for finely tuning the punch Position in hole.

2. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the diameter of the box body is 1cm- 1m, the material of the box body are one or both of polypropylene and polystyrene.

3. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the bottom of the box body and described Adhesive layer is additionally provided between micro-fluidic chip molding die, the adhesive layer is for fixing the micro-fluidic chip molding die.

4. the producing device of micro-fluidic chip according to claim 3, which is characterized in that the material of the adhesive layer is poly- Dimethyl siloxane.

5. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the side wall of the box body is equipped with height Spend graduation mark.

6. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the diameter of the box cover is 1cm- 1m, the material of the box cover are one or both of polypropylene and polystyrene.

7. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the three-dimensional trim holder is removable It is located at unloading on the box cover.

8. the producing device of micro-fluidic chip according to claim 1, which is characterized in that the diameter of the punch is 1 μ m-100mm；

Alternatively, the molding hole is multiple, the punch and molding hole one-to-one correspondence；

Alternatively, the punch is made or is made of rigid plastics material using metal material.

9. a kind of production method of micro-fluidic chip, which is characterized in that use the micro-fluidic core as described in claim any one of 1-8 The producing device of piece makes, and production method specifically comprises the following steps:

By the micro-fluidic chip molding die fixed placement in the bottom of the box body；

The box cover is connected through a screw thread and is fastened with the box body；

The punch is inserted into the molding hole of the micro-fluidic chip molding die by the lid mouth, and using three-dimensional The adjustment frame comprehensive fine tuning punch, so that the punch is directed at fitting with the molding hole；

The support chip is set on the box cover and is supported and secures the above punch；

Performed polymer is added in the forming cavity of the box body by the lid mouth；

The box body that performed polymer is added is subjected to heating, drying processing, is cooled to room temperature after heating；

After the support chip is dismantled from the box cover, the punch is extracted vertically, completes the system of micro-fluidic chip mesoporous Make.

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