CN106893671A - Micro-organism test apparatus - Google Patents
Micro-organism test apparatus Download PDFInfo
- Publication number
- CN106893671A CN106893671A CN201710209409.8A CN201710209409A CN106893671A CN 106893671 A CN106893671 A CN 106893671A CN 201710209409 A CN201710209409 A CN 201710209409A CN 106893671 A CN106893671 A CN 106893671A
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- micro
- module
- culture
- test apparatus
- ccd sensor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Molecular Biology (AREA)
- Computer Hardware Design (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of micro-organism test apparatus, its culture apparatus includes being transparent casing and case lid, and multiple culture tanks are set on casing, and the bottom of casing embeds fluorescent optical sensor at culture tank lower section；Optical sampling component includes main body frame, LASER Light Source, ccd sensor, main body frame is that upper end is uncovered structure, lower end closed and have round-meshed columnar structured, LASER Light Source irradiates located at one end of main body frame closing to uncovered structure one end, and ccd sensor is on the circular hole；Optical sampling component also includes image capture module, FPGA module, CCD drive circuits and wireless communication module, FPGA module is connected by CCD drive circuits with ccd sensor, the picture signal of ccd sensor scanning is connected by image capture module with FPGA module, and FPGA module is connected by wireless communication module with intelligent mobile terminal；Board is provided with video installing port and culture apparatus mounting table.
Description
Technical field
The present invention relates to microorganism detection field, more particularly to a kind of micro-organism test apparatus.
Background technology
With expanding economy, the continuous improvement of people's living standard, the consciousness for food security is also carried constantly
It is high.At present, the method for the detection of microorganism, including use immune measurement method, ELISA method, gas-chromatography mass analysis, liquid phase
The machines such as chromatogram mass analysis survey method, but the pre-treatment for determining is numerous and diverse, and the precision of judgement can not be said fully.These detection sides
Method before testing, is required to cultivate in advance, that is, spend and go culture more for a long time including the microorganism including spoilage organisms.
Using the technology including marking microorganism, to protrude between the light that microorganism sends and the light that growth holder sends
Contrast.Due to the sensitive optical system of the feature (such as wavelength or intensity) using the light to sending, so using specific
Fluorescence labeling, fluorogen or using the enzyme that can expose the bioluminescence sent by such as ATP, can carry out early stage to microorganism
Detection.
Additionally, it is most common and one of most ancient method to be cultivated by agar medium, wherein culture medium is for one
Plant or several microorganism types are more or less selectable.In a period of it generally can extend to several days, the culture medium is suitable
Cultivated at a temperature of, for growing the microorganism to be found.The shortcoming of this cultural method is still to be formed on agar
Bacterium colony can carry out the visible detection of bore hole, it is necessary to relatively long cultivation cycle.Be will also realize that from current technological level, can be with
Chain reaction (also referred to as PCR breedings) is performed by the breeding of DNA sequence dna or RNA sequence, so that it is determined that existing in sample specific
Microorganism.The shortcoming of these methods is：In order that the bacterium colony formed on agar medium can carry out the visible detection of bore hole, need
Want relatively long cultivation cycle.And be will also realize that from current technological level, can be by the breeding of NDA sequences or RNA sequence
Chain polymerization reaction (also referred to as PCR breedings) is performed, so that it is determined that the specified microorganisms in sample.The shortcoming of these methods is desirable
Several DNA, i.e., several microorganism generally requires at least tens kinds microorganisms, and this method is not based on the side of growth
Method is sensitive.
Above-mentioned agar medium or nutrient solution are cultivated, and look into method by vision or fluorescence labeling is identified, and
Visual examination technique includes the turbidity and color change of the suspended material in monitoring nutrient solution, and the efficiency comparison of the two methods is low
Under, and can only rough Statistics, accuracy rate is less high.
The content of the invention
It is an object of the invention to provide a kind of micro-organism test apparatus.
To achieve these goals, the technical scheme of present invention offer is：A kind of micro-organism test apparatus are provided, including：
Culture apparatus, the culture apparatus includes being transparent casing and case lid, and multiple cultures are set on the casing
Groove, and the bottom of the casing is located at embedded fluorescent optical sensor at culture tank lower section；
Optical sampling component, the optical sampling component includes main body frame, LASER Light Source, ccd sensor, the main body
Framework upper end be uncovered structure, lower end closed and have round-meshed columnar structured, the LASER Light Source be located at the body frame
One end of frame closing and irradiated to described uncovered structure one end, the ccd sensor is on the circular hole；The optical sampling
Component also includes image capture module, FPGA module, CCD drive circuits and wireless communication module, and the FPGA module passes through institute
State CCD drive circuits to be connected with the ccd sensor, the picture signal of the ccd sensor detection passes through image capture module
It is connected with the FPGA module, the FPGA module is connected by wireless communication module with intelligent mobile terminal；
Board, the board is provided with video installing port and culture apparatus mounting table, is opened up on the culture apparatus mounting table
There is the groove for placing the culture apparatus, the groove is located at directly over the optical sampling component, the intelligent mobile
Terminal is installed on the video installing port.
The FPGA module is also associated with USB3.0 interfaces, and the FPGA module can also be by USB3.0 interfaces and intelligence
Mobile terminal is connected.
The case lid just to being provided with boss at the culture tank, by the boss realized and the culture tank by the case lid
Be tightly connected.
Light source optical filter is additionally provided with the LASER Light Source.
Launching light optical filter and optical system are additionally provided with the ccd sensor, the light source optical filter and launching light are filtered
Mating plate filters the light of same frequency, and the ccd sensor realizes the inspection to the fluorescent optical sensor by the optical system
Survey.
The coarse structure of the main body frame inner surface for the benefit of light scattering.
Compared with prior art, due in micro-organism test apparatus of the present invention, including optical sampling component and board, institute
State board and be provided with video installing port and culture apparatus mounting table, the culture apparatus is put after culture well in outside insulating box
The culture apparatus mounting table is placed in, the optical sampling component can be by the FPGA module and by wireless communication module
It is connected with the intelligent mobile terminal, and testing result is shown and preserved in the intelligent mobile terminal.
By following description and with reference to accompanying drawing, the present invention will become more fully apparent, and these accompanying drawings are used to explain the present invention
Embodiment.
Brief description of the drawings
Fig. 1 is the schematic diagram of one embodiment of the board of micro-organism test apparatus of the present invention.
Fig. 2 is that the culture apparatus of micro-organism test apparatus as shown in Figure 1 is positioned over one of optical sampling component implementation
The schematic diagram of example.
Fig. 3 is the circuit theory module map of micro-organism test apparatus as shown in Figure 1.
Specific embodiment
With reference now to Description of Drawings embodiments of the invention, the element numbers being similar in accompanying drawing represent similar element.Such as
It is upper described, as shown in Figures 1 to 3, micro-organism test apparatus provided in an embodiment of the present invention, including：
Culture apparatus 1, the culture apparatus 1 includes being transparent casing 10 and case lid 11, is set on the casing 10
Multiple culture tanks 12, and the bottom of the casing 10 is located at embedded fluorescent optical sensor 13 at the lower section of the culture tank 12；
Optical sampling component 2, the optical sampling component 2 includes main body frame 20, LASER Light Source 21, ccd sensor 22,
The upper end of the main body frame 20 be uncovered structure, lower end closed and have round-meshed columnar structured, the LASER Light Source 21 sets
Irradiated to described uncovered structure one end in one end of the closing of the main body frame 20, the ccd sensor 22 is located at the circle
Kong Shang；The optical sampling component 2 also includes image capture module 23, FPGA module 24, CCD drive circuits 25 and wireless telecommunications
Module 26, the FPGA module 24 is connected by the CCD drive circuits 25 with the ccd sensor 22, the ccd sensor
The picture signal of 22 detections is connected by image capture module 23 with the FPGA module 24, and the FPGA module 24 is by wireless
Communication module 26 is connected with intelligent mobile terminal；In the present embodiment, intelligent mobile terminal can be Android, apple or
Windows flat boards.The picture signal of the detection of the ccd sensor 22 passes to the FPGA by described image acquisition module 23
Module 24, the wireless communication module 26 can be bluetooth module, wifi module.
Board 3, the board 3 is provided with video installing port 30 and culture apparatus mounting table 31, the culture apparatus mounting table
The groove 32 for placing the culture apparatus 1 is offered on 31, the groove 32 is just gone up positioned at the optical sampling component 2
Side, the intelligent mobile terminal is installed on the video installing port 30.
In embodiment as shown in Figure 3, the FPGA module 24 is also associated with USB3.0 interfaces 27, the FPGA module 24
Can also be connected with intelligent mobile terminal by USB3.0 interfaces 27.
In embodiment as shown in Figure 2, the case lid 11 is just to being provided with boss 14, the case lid 11 at the culture tank 12
Realize being tightly connected with the culture tank 12 by the boss 14.
It should be noted that the multiple culture tanks 12 in the culture apparatus 1 are separate, the culture tank 12
Just the sterile culture environment containing culture medium, and each culture tank 12 be it is separate without mutual infection, need to
The sample solution to be cultivated is injected into the culture tank 12 by syringe from the boss 14, culture tank 12 described in which part
Not sample injection solution, it is as object of reference.
Additionally, the difference of the sample type that the culture apparatus 1 is cultivated as needed, can select to be trained at normal temperatures
Support, it is also possible to which selection is cultivated in insulating box.Treat that the sample in the culture apparatus 1, according to training status, reaches regulation
Incubation time after, be placed on the culture apparatus mounting table 31 on the board 3, be limited in the groove 32
On, be scanned by the optical sampling component 2, and due to the board 3 be with the culture apparatus 1 be lift-off, because
This operating personnel extremely easily can be opened up using the board 3 when using the present invention to the personnel for needing to be demonstrated
Show.
In embodiment as shown in Figure 2, light source optical filter 28 is additionally provided with the LASER Light Source 21.
In embodiment as shown in Figure 2, launching light optical filter 29 and optical system are additionally provided with the ccd sensor 22
29a, the light source optical filter 28 and launching light optical filter 29 filter the light of same frequency, and the ccd sensor 22 passes through institute
State detection of the optical system 29a realizations to the fluorescent optical sensor 13.
In embodiment as shown in Figure 2, the coarse structure of the inner surface of the main body frame 20 for the benefit of light scattering.
Compared with prior art, with reference to Fig. 1~3, due in micro-organism test apparatus of the present invention, including optical sampling group
Part 2 and board 3, the board 3 are provided with video installing port 30 and culture apparatus mounting table 31, and the culture apparatus 1 is outside permanent
After being cultivated well in incubator, the culture apparatus mounting table 31 is positioned over, the optical sampling component 2 can be by the FPGA
Module 24 and it is connected with the intelligent mobile terminal by wireless communication module 26, and by testing result at the intelligent mobile end
End is shown and is preserved.
Above disclosed is only the preferred embodiments of the present invention, can not limit the right of the present invention with this certainly
Scope, therefore the equivalent variations made according to scope of the present invention patent, still belong to the scope that the present invention is covered.
Claims (8)
1. a kind of micro-organism test apparatus, it is characterised in that including：
Culture apparatus, the culture apparatus includes being transparent casing and case lid, and multiple culture tanks are set on the casing, and
The bottom of the casing is located at culture tank lower section and embeds fluorescent optical sensor；
Optical sampling component, the optical sampling component includes main body frame, LASER Light Source, ccd sensor, the main body frame
Upper end is uncovered structure, lower end closed and has round-meshed columnar structured, and the LASER Light Source is sealed located at the main body frame
One end for closing and irradiated to described uncovered structure one end, the ccd sensor is on the circular hole；The optical sampling component
Also include image capture module, FPGA module, CCD drive circuits and wireless communication module, the FPGA module passes through the CCD
Drive circuit is connected with the ccd sensor, the picture signal of ccd sensor scanning by image capture module with it is described
FPGA module is connected, and the FPGA module is connected by wireless communication module with intelligent mobile terminal；
Board, the board is provided with culture apparatus mounting table, is offered for placing the training on the culture apparatus mounting table
The groove of device is supported, the groove is located at directly over the optical sampling component.
2. micro-organism test apparatus as claimed in claim 1, it is characterised in that the board is additionally provided with video installing port, institute
Intelligent mobile terminal is stated to install on the video installing port.
3. micro-organism test apparatus as claimed in claim 1, it is characterised in that the FPGA module is also associated with USB3.0 and connects
Mouthful, the FPGA module can also be connected by USB3.0 interfaces with intelligent mobile terminal.
4. micro-organism test apparatus as claimed in claim 1, it is characterised in that the groove is being located at the uncovered structure just
Top.
5. micro-organism test apparatus as described in any one of Claims 1 to 4, it is characterised in that the case lid is just to the training
Support and be provided with boss at groove, the case lid is realized being tightly connected with the culture tank by the boss.
6. micro-organism test apparatus as claimed in claim 1, it is characterised in that light source optical filtering is additionally provided with the LASER Light Source
Piece.
7. micro-organism test apparatus as claimed in claim 6, it is characterised in that be additionally provided with launching light on the ccd sensor
Optical filter and optical system, the light source optical filter and launching light optical filter filter the light of same frequency, the ccd sensor
Detection to the fluorescent optical sensor is realized by the optical system.
8. micro-organism test apparatus as claimed in claim 1, it is characterised in that the main body frame inner surface for the benefit of light
The coarse structure of scattering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710209409.8A CN106893671A (en) | 2017-03-31 | 2017-03-31 | Micro-organism test apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710209409.8A CN106893671A (en) | 2017-03-31 | 2017-03-31 | Micro-organism test apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106893671A true CN106893671A (en) | 2017-06-27 |
Family
ID=59193550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710209409.8A Pending CN106893671A (en) | 2017-03-31 | 2017-03-31 | Micro-organism test apparatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN106893671A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975290A (en) * | 2018-11-30 | 2019-07-05 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A rapid analyzer for bacterial detection |
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2017
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109975290A (en) * | 2018-11-30 | 2019-07-05 | 军事科学院军事医学研究院环境医学与作业医学研究所 | A rapid analyzer for bacterial detection |
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Application publication date: 20170627 |