CN106834088A - A kind of cell harvestor in bioreactor - Google Patents
A kind of cell harvestor in bioreactor Download PDFInfo
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- CN106834088A CN106834088A CN201710198654.3A CN201710198654A CN106834088A CN 106834088 A CN106834088 A CN 106834088A CN 201710198654 A CN201710198654 A CN 201710198654A CN 106834088 A CN106834088 A CN 106834088A
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- separator
- settler
- delivery pipe
- cylindrical drum
- installing port
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of cell harvestor in bioreactor, including the reactor for depositing culture mixed liquor, end cap is provided with the opening of reactor upper end, installing port is offered on end cap, settler screw rod is arranged with installing port, settler screw rod is made up of delivery pipe with the cylindrical drum for being arranged on delivery pipe lower end, and outside is stretched into delivery pipe upper end through installing port, and cylindrical drum is stretched into inside culture medium；Separator is provided with inside cylindrical drum, separator includes multiple separators being arranged in order, separator is vertically equidistantly arranged, and connected by fix bar between separator, fix bar is uniformly distributed along separator circumferencial direction, gap is provided between two neighboring separator, fix bar upper end is fixed with cylindrical drum top, separator is cone, and the " V "-shaped shape in separator section, the discharge gate being obliquely installed is provided with separator lower end, charging aperture is communicated with the separator outlet of bottom.
Description
Technical field
The present invention relates to detection blower fan vibrations field, specifically a kind of cell in bioreactor is collected
Device.
Background technology
General by the way of perfusion culture in cell microcarrier incubation, so-called perfusion culture is exactly while adding
Culture medium, while extracting culture medium, the purpose of its supplementing culture medium is for cell growth provides enough nutrition, the purpose of extraction
It is to reduce the harmful components in culture medium, during cell growth, cell can be metabolized out some harmful substances and be dispersed in training
In foster base, while culture medium is extracted, it is desirable to which the microcarrier of attached cell can be retained in tank body, but these nuisances
Matter is difficult to be separated with microcarrier, and existing technology is easy to together with harmful substance take microcarrier away, increases cost.
The content of the invention
The technical problems to be solved by the invention are, for the shortcoming of above prior art, propose a kind of for biological anti-
The cell harvestor in device is answered, is not only simple in structure, it is easy to operate, operating efficiency is improved, low cost is safe and reliable, uses the longevity
Life length, economize on resources, can utilize precipitation principle, extract culture medium during, due to attached cell microcarrier with
There is a difference in the density of culture medium, a period of time is taken using pumping, then keep period time of suspension by attached cell
Microcarrier natural subsidence, to reach the purpose that microcarrier with culture medium separate, then again toward adding new culture medium in reactor,
Greatly reduce the purity of harmful substance, be conducive to the culture of cell.
In order to solve the above-mentioned technical problem, the technical scheme is that being accomplished by the following way：One kind is for giving birth to
Cell harvestor in thing reactor, including for depositing the reactor of culture mixed liquor, be provided with the opening of reactor upper end
End cap, offers installing port on end cap, settler screw rod is arranged with installing port, and settler screw rod is by delivery pipe and is arranged on
Outside is stretched into the cylindrical drum composition of delivery pipe lower end, delivery pipe upper end through installing port, and cylindrical drum is stretched into inside culture medium；
Separator is provided with inside cylindrical drum, separator includes multiple separators being arranged in order, and separator is along side vertically
To equidistantly arrangement, and connected by fix bar between separator, fix bar is uniformly distributed along separator circumferencial direction, adjacent two
Gap is provided between individual separator, fix bar upper end is fixed with cylindrical drum top, separator is in for cone, and separator section
" V " word shape, the discharge gate being obliquely installed is provided with separator lower end, and charging aperture is communicated with the separator outlet of bottom.
So, by technical scheme, separator is provided with inside cylindrical drum, separator includes separator,
It is conical structure using separator, because the microcarrier of attached cell has a difference with the density of culture medium, at itself
Microcarrier can fall in the presence of gravity, depart from culture medium, i.e. microcarrier and separated with culture medium, then again toward addition in reactor
New culture medium, greatly reduces the purity of harmful substance, is conducive to the culture of cell, meets the demand of client, and the structure
Simply, easy to operate, low cost is safe and reliable, and the " V "-shaped shape in separator section, when external suction equipment starts,
Because separator exterior space is than larger, separator inner space is smaller, and culture mixed liquor is entered by separator both sides
In delivery pipe, when external equipment stops, microcarrier is separated into separator in the presence of self gravitation, then by discharge
Mouth discharge, whole process is simple, easy to operate, rational in infrastructure.
The technical scheme that further limits of the invention is：
The cell harvestor in bioreactor is previously described for, sealing device is additionally provided with end cap, sealing device includes and peace
The settler interface that dress mouth is fixed, settler interface is arranged with adapter sleeve, and adapter sleeve is fixedly connected with delivery pipe, under adapter sleeve
End is extended with limited step, and the threaded upper ends for being located at adapter sleeve in delivery pipe are connected with locking nut, and settler interface outside
Settler nut is threaded with, a sealing device is set on end cap can be isolated from the outside inside reactor, it is to avoid empty
Gas enters internal, influences the quality of culture medium, and the sealing structure is ingenious, reliable, long service life, low cost.
The cell harvestor in bioreactor is previously described for, sealing is provided between locking nut and settler interface
Pad, is provided with the centre position of the first sealing ring, adapter sleeve and settler interface contact between limited step and settler interface
The second sealing ring is provided with, increases sealing property.
The beneficial effects of the invention are as follows：Separator is provided with inside cylindrical drum, separator includes separator, using separation
Device is conical structure, because the microcarrier of attached cell has a difference with the density of culture medium, in the work of self gravitation
Can be fallen with lower microcarrier, depart from culture medium, i.e. microcarrier and separated with culture medium, then again toward adding new culture in reactor
Base, greatly reduces the purity of harmful substance, is conducive to the culture of cell, meets the demand of client, and the simple structure, operation
Convenient, low cost is safe and reliable, and the " V "-shaped shape in separator section, when external suction equipment starts, due to separating
Device exterior space is than larger, and separator inner space is smaller, and culture mixed liquor is entered in delivery pipe by separator both sides,
When external equipment stops, microcarrier is separated into separator in the presence of self gravitation, is then discharged by discharge gate, whole
Individual process is simple, easy to operate, rational in infrastructure.
Brief description of the drawings
Fig. 1 is front view of the invention；
Fig. 2 is the direction view of A in Fig. 1；
Fig. 3 is the close-up schematic view of B in Fig. 1；
Wherein：1- reactors, 2- end caps, 3- installing ports, 4- delivery pipes, 5- cylindrical drums, 6- separators, 7- fix bars, 8- gaps,
9- discharge gates, 10- settler interfaces, 11- adapter sleeves, 12- limited steps, 13- locking nuts, 14- settler nuts, 15- is close
Packing, the sealing rings of 16- first, the sealing rings of 17- second.
Specific embodiment
The present invention is described in further detail below：
Embodiment 1
A kind of cell harvestor in bioreactor that the present embodiment is provided, including for depositing the anti-of culture mixed liquor
Device 1 is answered, end cap 2 is provided with the opening of the upper end of reactor 1, installing port 3 is offered on end cap 2, sedimentation is arranged with installing port 3
Device screw rod, settler screw rod is made up of delivery pipe 4 with the cylindrical drum 5 for being arranged on the lower end of delivery pipe 4, and the upper end of delivery pipe 4 passes through peace
Dress mouth 3 stretches into outside, and cylindrical drum 5 is stretched into inside culture medium；
Separator is provided with inside cylindrical drum 5, separator includes nine separators 6 being arranged in order, and separator 6 is along vertical
Direction equidistantly arranges, and is connected by fix bar 7 between separator 6, and fix bar 7 is uniformly distributed along the circumferencial direction of separator 6
Four, gap 8 is provided between two neighboring separator 6, the upper end of fix bar 7 is fixed with the top of cylindrical drum 5, separator 6 is circular cone
Shape, and the " V "-shaped shape in the section of separator 6, are provided with the discharge gate 9 being obliquely installed in separator lower end, charging aperture 9 with most under
The outlet of separator 6 at end is communicated.
Sealing device is additionally provided with end cap 2, sealing device includes the settler interface 10 fixed with installing port 3, settler
Interface 10 is arranged with adapter sleeve 11, and adapter sleeve 11 is fixedly connected with delivery pipe 4, and limited step is extended with the lower end of adapter sleeve 11
12, the threaded upper ends for being located at adapter sleeve 11 in delivery pipe 4 are connected with locking nut 13, and the outside screw of settler interface 10 is connected
There is settler nut 14；A sealing device is set on end cap can be isolated from the outside inside reactor, it is to avoid air enters
Inside, influences the quality of culture medium, and the sealing structure is ingenious, reliable, long service life, low cost；Locking nut 13 with
Sealing gasket 15 is provided between settler interface 10, the first sealing ring 16 is provided between limited step 12 and settler interface 10,
The centre position that adapter sleeve 11 is contacted with settler interface 10 is provided with the second sealing ring 17.
The course of work of the present embodiment：
During culture medium first will be stretched into for the separator of the cell harvestor lower end in bioreactor；Outside is then turned on to take out
Device is inhaled, is aspirated 1-60 second, then allow to cultivate mixed liquor static 1-60 seconds in separator, due to microcarrier mass ratio in itself
Culture medium is big, under gravity can be slowly separated with culture medium, and by after separator, finally being arranged from discharge gate
Go out, after microcarrier is separated with culture medium, the culture medium that then will be separated in startup external equipment is extracted to outside,
Greatly reduce the harmful substance in reactor, then repeatedly above step, then add new culture medium.
The technical scheme of this sample embodiment is not only simple in structure, easy to operate, improves operating efficiency, low cost, safety
Reliable, long service life economizes on resources, and the principle of precipitation can be utilized, during culture medium is extracted, due to attached cell
The density of microcarrier and culture medium there is a difference, take a period of time using pumping, then keep period time of suspension to allow
The microcarrier natural subsidence of attached cell, to reach the purpose that microcarrier with culture medium separate, then again toward addition in reactor
New culture medium, greatly reduces the purity of harmful substance, is conducive to the culture of cell.
Above example is only explanation technological thought of the invention, it is impossible to limit protection scope of the present invention with this, every
According to technological thought proposed by the present invention, any change done on the basis of technical scheme each falls within the scope of the present invention
Within.
Claims (3)
1. a kind of cell harvestor in bioreactor, including the reactor that mixed liquor is cultivated for depositing（1）, it is described
Reactor（1）End cap is provided with the opening of upper end（2）, it is characterised in that：The end cap（2）On offer installing port（3）, institute
State installing port（3）In be arranged with settler screw rod, the settler screw rod is by delivery pipe（4）Be arranged on delivery pipe（4）Lower end
Cylindrical drum（5）Composition, the delivery pipe（4）Upper end passes through installing port（3）Stretch into outside, the cylindrical drum（5）Stretch into culture
Inside base；
In the cylindrical drum（5）Inside is provided with separator, and the separator includes multiple separators being arranged in order（6）,
The separator（6）Vertically equidistantly arrangement, and separator（6）Between pass through fix bar（7）Connection, the fix bar
（7）Along separator（6）Circumferencial direction is uniformly distributed, two neighboring separator（6）Between be provided with gap（8）, the fix bar（7）
Upper end and cylindrical drum（5）Top is fixed, the separator（6）It is cone, and separator（6）The " V "-shaped shape in section, in institute
State separator lower end and be provided with the discharge gate being obliquely installed（9）, the charging aperture（9）With the separator of bottom（6）Outlet phase
It is logical.
2. the cell harvestor in bioreactor according to claim 1, it is characterised in that：The end cap（2）
On be additionally provided with sealing device, the sealing device includes and installing port（3）Fixed settler interface（10）, the settler connects
Mouthful（10）It is arranged with adapter sleeve（11）, the adapter sleeve（11）With delivery pipe（4）It is fixedly connected, in the adapter sleeve（11）Lower end
It is extended with limited step（12）, in the delivery pipe（4）Positioned at adapter sleeve（11）Threaded upper ends be connected with locking nut（13）,
And the settler interface（10）Outside screw is connected with settler nut（14）.
3. the cell harvestor in bioreactor according to claim 2, it is characterised in that：The locking nut
（13）With settler interface（10）Between be provided with sealing gasket（15）, in the limited step（12）With settler interface（10）Between
It is provided with the first sealing ring（16）, the adapter sleeve（11）With settler interface（10）The centre position of contact is provided with the second sealing ring
（17）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710198654.3A CN106834088A (en) | 2017-03-29 | 2017-03-29 | A kind of cell harvestor in bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710198654.3A CN106834088A (en) | 2017-03-29 | 2017-03-29 | A kind of cell harvestor in bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106834088A true CN106834088A (en) | 2017-06-13 |
Family
ID=59142793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710198654.3A Pending CN106834088A (en) | 2017-03-29 | 2017-03-29 | A kind of cell harvestor in bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106834088A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107541464A (en) * | 2017-10-31 | 2018-01-05 | 山东亦度生物技术有限公司 | The retention method of enhanced cell microcarrier perfusion culture |
| WO2024056037A1 (en) * | 2022-09-14 | 2024-03-21 | 北京华龛生物科技有限公司 | Liquid drawing device, bioreactor, and liquid drawing method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006627A1 (en) * | 1989-11-03 | 1991-05-16 | Massachusetts Institute Of Technology | Nested-cone separator |
| US5320963A (en) * | 1992-11-25 | 1994-06-14 | National Research Council Of Canada | Bioreactor for the perfusion culture of cells |
| JPH078265A (en) * | 1993-06-23 | 1995-01-13 | Sumitomo Pharmaceut Co Ltd | Microcarrier separation device and separation method |
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN201933090U (en) * | 2010-11-22 | 2011-08-17 | 朱学仁 | Interception device for perfusion culture of cell microcarrier |
| CN102337200A (en) * | 2010-07-20 | 2012-02-01 | 北京心意圆成生物科技有限公司 | High-efficiency microcarrier cell-culture medium separating device |
| CN104126001A (en) * | 2012-02-20 | 2014-10-29 | 拜耳技术服务有限公司 | One-way separator for retaining and recirculating cells |
-
2017
- 2017-03-29 CN CN201710198654.3A patent/CN106834088A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006627A1 (en) * | 1989-11-03 | 1991-05-16 | Massachusetts Institute Of Technology | Nested-cone separator |
| US5320963A (en) * | 1992-11-25 | 1994-06-14 | National Research Council Of Canada | Bioreactor for the perfusion culture of cells |
| JPH078265A (en) * | 1993-06-23 | 1995-01-13 | Sumitomo Pharmaceut Co Ltd | Microcarrier separation device and separation method |
| CN101653749A (en) * | 2009-09-21 | 2010-02-24 | 北京天广实生物技术股份有限公司 | Plate-type settling centrifuge for perfusion |
| CN102337200A (en) * | 2010-07-20 | 2012-02-01 | 北京心意圆成生物科技有限公司 | High-efficiency microcarrier cell-culture medium separating device |
| CN201933090U (en) * | 2010-11-22 | 2011-08-17 | 朱学仁 | Interception device for perfusion culture of cell microcarrier |
| CN104126001A (en) * | 2012-02-20 | 2014-10-29 | 拜耳技术服务有限公司 | One-way separator for retaining and recirculating cells |
| US20150017716A1 (en) * | 2012-02-20 | 2015-01-15 | Bayer Intellectual Property Gmbh | One-way separator for retaining and recirculating cells |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107541464A (en) * | 2017-10-31 | 2018-01-05 | 山东亦度生物技术有限公司 | The retention method of enhanced cell microcarrier perfusion culture |
| WO2024056037A1 (en) * | 2022-09-14 | 2024-03-21 | 北京华龛生物科技有限公司 | Liquid drawing device, bioreactor, and liquid drawing method |
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| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170613 |
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| RJ01 | Rejection of invention patent application after publication |