CN106823475B - a blood diverter - Google Patents

Abstract

The invention provides a blood shunt which mainly comprises a main body and a fluid shunt channel. The method is characterized in that: the main body is disc-shaped, and fluid shunting channels which are spiral, closed up and down and unequal in width are processed from the edge of the disc to the center of a circle. The fluid diversion channel of the main body is more than or equal to 3 layers. A section of drainage dike is processed on a channel central line at the beginning of each layer of the diversion channel, diversion island walls with the number more than or equal to 3 are processed at the downstream of the drainage dike, the diversion channel is divided into an inner flow channel and an outer flow channel from the diversion port by the diversion island walls, a convex rotary collision wall is processed at each inner flow channel, the downstream of the rotary collision wall is next to a separation port, and the separation port is arranged between the two diversion island walls and communicates the inner flow channel with the outer flow channel. The cell separation device has the advantages that the cell separation device can be used for rapidly separating cells, is simple and convenient to operate and is not easy to cause cell damage, liquid can be fed into the cell separation device while the cell separation device is used for separating cells, the cells can be separated repeatedly and circularly, and the separation efficiency is improved.

Description

a blood diverter

technical field

The invention relates to a blood separation device, which is a blood shunt suitable for common medical institutions to separate blood cells in terms of use.

technical background

At present, there are roughly two technical methods for blood cell separation, namely membrane separation technology and centrifugal separation technology. Membrane separation technology uses a thin film with selective permeability as the separation medium. The raw liquid passes through one side of the membrane under a certain pressure. To achieve the purpose of material separation and concentration. Membrane separation technology adopts non-cross-flow filtration, and the membrane is easy to be blocked. Using cross-flow filtration, although the membrane is not easy to be blocked, the performance requirements of the membrane are high and the cost increases. Centrifugal separation technology is to separate the blood components from each other according to the difference in specific gravity through centrifuge separation equipment to obtain the required blood components, but it is easy to cause mechanical damage to the cells and easy to pollute, and the cells have phase changes, which affects the quality of the cells; at the same time , the environmental requirements of the laboratory are very strict, which increases the cost of cell separation.

Therefore, the present invention solves this problem, and provides a simple and efficient blood shunt technical solution with minimum cell loss, no cell phase change, and convenience for subsequent analysis.

Contents of the invention

In order to solve the above problems, the technical solution of the present invention is: a blood diverter, mainly composed of a main body and a fluid diversion channel. It is characterized in that: the main body is in the shape of a disc, and there are helical, up and down closed fluid distribution channels with unequal width processed from the edge of the disc to the center of the circle. The fluid distribution channel of the main body is more than or equal to 3 layers. The first layer of distribution channel starts from the total inlet processed on the edge of the disc and ends at the outlet of the first layer of outer flow channel. A section of diversion dike is processed on the center line of the channel at the beginning of each layer of diversion channel; downstream of the diversion dike is processed with a number of ≥ 3 diversion island walls, and the diversion island wall divides the diversion channel from the diversion port into an inner flow channel and an outer flow Road; each inner runner is processed with a protruding swirl impact wall, and the downstream of the swirl impact wall is next to a sorting port; the sorting port is between the two diversion island walls, connecting the inner flow channel with the outer flow channel. Above 1 roundabout collision wall and 2 diversion island walls form a sorting unit. The inlet of the inner flow channel next to the sorting port becomes the splitting port of the next sorting unit again. The actual design is based on the unit of sorting unit, repeated layered sorting. The second layer of shunt channel starts from the drainage dike of the second layer and ends at the outlet of the second layer of the outer flow channel. The third layer of shunt channel starts from the drainage dike of the third layer and ends at the outlet of the third layer of the outer flow channel. The fluid distribution channels of the following layers are analogized in turn, and finally, the inner flow channel ends at the total sample outlet at the center of the main body.

In the above technical solution, the drainage embankment is a strip with a width of 0.5 mm to 1 mm and a height of 2 mm to 5 mm lower than the top of the fluid distribution channel. The width of the fluid distribution channels in each layer is consistent, and the width dimension is ≥ 5 mm, or the width of the fluid distribution channels in each layer increases or decreases layer by layer. The said diversion island wall is fish-shaped, wavy, or rhombus-shaped along the direction of the fluid diversion channel. This design makes the arc direction of the inner flow channel of a sorting unit inconsistent in the front and rear sections of the channel; or it is fan-shaped or horn-shaped. , this design makes the arc direction of the inner channel of a sorting unit consistent in the front and rear sections of the channel. The height of the diversion island wall is flush with the top of the fluid diversion channel, that is, the fluid diversion channel is closed up and down.

In the above technical solution, the outer flow channel is an arc-shaped channel with smooth and smooth wall surfaces on both sides and a smooth transition along the arc of the main body in the same direction; the outer flow channels of each layer have the same width, and the width dimension is ≤ 1/4 of the fluid diversion channel width dimension, or The width dimensions of the outer runners of each layer gradually decrease or gradually increase.

In the above technical solution, the widths of the inner runners in the front, middle and rear sections of a sorting unit are inconsistent, and the widths of the inner runners in each layer are inconsistent. The shape of the inner flow channel: because the first half of the side of the inner flow channel of the diversion island wall is in the arc shape of the arc of the main body in the opposite direction, and in the second half of the convex surface corresponding to the swinging wall, it is in the same direction as the arc of the main body arc; and because the protruding part of the wall hits the wall is the opposite direction of the arc of the main body. That is to say, the diversion island wall is matched with the whirling and bumping wall, so that the first half of the inner flow channel forms an arc-shaped roundabout that is opposite to the arc of the main body. Or the shape of the inner runner: because the side of the diversion island wall and the whirling wall are both opposite to the arc direction of the main body, the inner runner is formed as a passage against the arc of the main body.

In the above technical solution, the diameters of the total sample inlet and the total sample outlet are consistent, and both are larger than the diameters of the outlets of the outer channels of each layer. The design ensures sufficient flow velocity in the inner runner. The outlet diameter of the outer channel of each layer needs to be measured and calculated by fluid simulation software according to the shape and size of the overall design. The height of the bottom of the outer runner of the outer runner is 0.5 mm to 2 mm higher than the bottom of the inner runner of the inner runner. The bottom of the outer runner and the bottom of the inner runner are at the sorting port, and are connected and transitioned by the bottom of the sorting port raised by a circular arc; Low sloped bottom.

In the above technical solution, the end of the diversion island wall facing the fluid and opposite to the direction of the fluid is a tapered diversion island wall tip. The tapered tip of the diversion island wall tip is sharpened into an end face with a width ≥ 1mm, and the end face is processed with a raised irregular convex surface from the side of the inner flow channel; the area of the irregular convex surface gradually goes up and down toward the side of the outer flow channel The bifurcation decreases, and the height gradually decreases, and finally merges with the irregular concave surface in the middle of the bifurcated fork on the irregular convex surface. On the side wall facing the fluid and opposite to the direction of the fluid, the whirling collision wall is processed with irregular convex surfaces and irregular concave surfaces of different sizes and uneven distribution. This design is to increase fluid swirl, increase fluid mixed flow and eddy flow, and reduce laminar flow phenomenon in the microchannel. To facilitate the separation of blood components and cells.

In the above technical solution, the main body can be processed with a dark barrier dike at the diversion port of the sorting unit; the dark barrier dike is a dike wall that is transversely penetrated and intercepted in the inner flow channel, and the height dimension is 2 mm to 5 mm lower than the top of the inner flow channel . This design is to increase the initial velocity of the fluid entering the inner channel. The diversion island wall of the main body can be processed with a sorting port dark dike at the sorting port; the sorting port dark dike is at the end of the diversion island wall facing away from the fluid and in the same direction as the fluid. It is intercepted at the sorting port, but does not completely pass through the embankment wall whose height dimension is 2mm~5mm lower than the top of the sorting port. This design is to increase the separation efficiency of the sorting port and further control the separation size.

The present invention uses the "flying sand" separation principle of Dujiangyan Water Conservancy, that is, uses the theory of swirling flow and centrifugal force, and applies this to the design of blood separation. At present, there are a lot of studies on the blood separation of microchannel chips at home and abroad. However, most of the microchannels are laminar flow, and the conventional fluid theory is not applicable. The present invention combines conventional water conservancy fluid with blood fluid, and applies it to the separation of blood components and cells in non-micron channels. Large-mass proteins and cells are separated at the sorting port. The separation of blood cells is achieved through multiple cycles of sorting.

The purpose of the present invention is to provide a cell separation device that can quickly separate cells, is easy to operate, and is not easy to cause cell damage. efficiency. The dispenser makes the operation of cell separation and collection simple and easy.

Description of drawings

Fig. 1 is a schematic top view of the present invention.

Fig. 2 is a schematic diagram of a sorting unit of the present invention.

Fig. 3 is a schematic view of the A-end surface of the wall tip of the diversion island of the present invention.

Fig. 4 is a D-D cross-sectional schematic view of the end face of the tip A of the diversion island wall of the present invention.

Fig. 5 is a schematic diagram of the end face B of the swinging wall of the present invention.

Fig. 6 is a schematic cross-sectional view of the drainage embankment of the present invention.

Fig. 7 is a schematic diagram of three sorting units of a fan-shaped diversion island wall of the present invention.

Fig. 8 is a schematic cross-sectional view of the sorting port A-A on the horizontal bottom surface of the outer channel bottom of the present invention.

Fig. 9 is a schematic cross-sectional view of the sorting port A-A on the inclined bottom surface of the outer channel bottom of the present invention.

Fig. 10 is a schematic diagram of three sorting units with dark barrier banks according to the present invention.

Fig. 11 is a B-B cross-sectional schematic view of the dark barrier bank of the present invention.

Fig. 12 is a schematic cross-sectional view of C-C of the submerged dike of the sorting port of the present invention.

In the figure: 1. The total injection port; 2. The first layer of split channel; 3. Drain bank; 4. Split port; 5. Split island wall; 6. Inner channel; 7. Outer channel; 8. Sorting port ;9. Whirling and hitting the wall; 10. Total sample outlet; 11. Main body; 12. Third-layer shunt channel; ; 16. Outlet of the first layer of outer flow channel; 17. Roundabout; 18. Concealed barrier embankment; 19. Concealed embankment of sorting port; 20. Distributor island wall tip; 21. Irregular convex surface; .The bottom of the inner runner; 24. The bottom of the sorting port; 25. The bottom of the outer runner.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

Embodiment one

Referring to the shape and structure of Fig. 1 to Fig. 9, a blood diverter, the number of fluid diversion channels of the main body 11 is 3 layers, and 9 diversion island walls 5 in each layer form 8 sorting units, that is, there are 8 sorting units. Mouth 8.

Each layer of fluid distribution channels has a depth of 8mm and a width of 10mm. The drainage embankment 3 is 1mm wide and 6mm high. The diversion island wall 5 is fish-shaped along the direction of the fluid diversion channel, and its height is 8 mm, which is flush with the top of the fluid diversion channel; the end face width of the diversion island wall tip 20 is 2 mm. The width of the outer runner 7 of each layer is 2.5 mm; the height of the bottom 25 of the outer runner of each layer is 2 mm higher than the height of the bottom 23 of the inner runner; the bottom 25 of the outer runner is a horizontal bottom surface after connection and transition. The inner runners 6 of each layer have horn openings at both ends, and the first half is an arc-shaped roundabout 17 with an arc arc of the opposite main body 11. The width of the end of the roundabout 17 is the narrowest; The channel is in the same direction as the arc of the main body 11. The diameters of the total sample inlet 1 and the total sample outlet 10 are ϕ2mm, and the diameters of the outlet 16 of the first layer of outer flow channel, the outlet 14 of the second layer of outer flow channel and the outlet 13 of the third layer of outer flow channel are ϕ0.9mm.

Embodiment two

Referring to the shape and structure of Figures 1 to 12, a blood diverter, the number of fluid diversion channels in the main body 11 is 5 layers, and 6 diversion island walls 5 in each layer form 5 sorting units, that is, there are 5 sorting units. Mouth 8.

The depth of the first layer of fluid distribution channel is 10mm, and the width is 12mm; the depth of the second layer of fluid distribution channel is 10mm, and the width is 11mm; the depth of the third layer of fluid distribution channel is 10mm, and the width is 10mm; The depth of the laminar fluid distribution channel is 10mm, and the width is 8mm. The drainage embankment 3 is 0.5mm wide and 5mm high. The diversion island wall 5 is fan-shaped along the direction of the fluid diversion channel, with a height of 10 mm flush with the top of the fluid diversion channel; the end face width of the diversion island wall tip 20 is 1 mm. The width of the first layer of outer runner 7 is 3mm; the width of the second layer of outer runner 7 is 2.5mm; the width of the third layer of outer runner 7 is 2mm; the width of the fourth layer of outer runner 7 is 1.5mm; the width of the fifth layer of outer runner 7 is 1.5mm. The height dimension of the outer runner bottom 25 of each layer is higher than the height of the inner runner bottom 23 by 1 mm; the outer runner bottom 25 is connected and transitioned to form an inclined bottom surface with a high transition point and a low outer side. The two ends of the inner runners 6 of each layer have horn openings, and are arc-shaped roundabouts 17 that are opposite to the arc of the main body 11, and the width of the end of the roundabout 17 is the narrowest. The diameters of the total sample inlet 1 and the total sample outlet 10 are ϕ3mm, and the diameters of the outlets of the first layer to the fifth layer of the outer flow channel are ϕ1.2mm.

Each branch port 4 of the sorting unit on the third and fifth floors is processed with a dark barrier bank 18 whose height dimension is 5 mm lower than the top of the inner flow channel 6 . At the separation port 8 of the diversion island wall 5 on the third and fifth floors, a sorting port dark dike 19 with a height dimension lower than the top of the sorting port 8 by 2mm is processed.

Embodiment three

Referring to the shape and structure of Figures 1 to 12, a blood diverter, the fluid diversion channel of the main body 11 = 4 layers, and 7 diversion island walls 5 in each layer form 6 sorting units, that is, there are 6 sorting ports 8.

The depth of the first layer of fluid distribution channel is 10mm, and the width is 9mm; the depth of the second layer of fluid distribution channel is 10mm, and the width is 10mm; the depth of the third layer of fluid distribution channel is 10mm, and the width is 11mm; the depth of the fourth layer of fluid distribution channel is 10mm, and the width is 12mm. The drainage embankment 3 is 0.5mm wide and 7mm high. The diversion island wall 5 is fish-shaped along the direction of the fluid diversion channel, and its height is 10 mm flush with the top of the fluid diversion channel; the end face width of the diversion island wall tip 20 is 1 mm. The width of the outer runner 7 of the first layer is 1.5mm; the width of the outer runner 7 of the second layer is 2mm; the width of the outer runner 7 of the third layer is 2.5mm; the width of the outer runner 7 of the fourth layer is 3mm. The height dimension of the outer runner bottom 25 of each layer is 1.5mm higher than the height of the inner runner bottom 23; the outer runner bottom 25 is an inclined bottom surface with a high transition point and a low outer side after connection and transition. The inner runners 6 of each layer have horn openings at both ends, and are arc-shaped roundabouts 17 with the arc arc of the main body 11. The width of the end of the roundabouts 17 is the narrowest; A channel that arcs toward the main body 11. The diameters of the total sample inlet 1 and the total sample outlet 10 are ϕ2.5mm, and the diameters of the outlets of the first layer to the fifth layer of the outer channel are ϕ1mm.

A dark barrier bank 18 whose height dimension is 3mm lower than the top of the inner flow channel 6 is processed at the diversion port 4 of the sorting unit of each layer. A sorting port dark embankment 19 whose height dimension is 3mm lower than the top of the sorting port 8 is processed at the sorting port 8 of the diverging island wall 5 of each layer.

Claims (6)

1. A blood shunt, mainly composed of a main body (11) and a fluid shunt channel, characterized in that: the main body (11) is in the shape of a disc, from the edge of the disc to the center of the circle, it is processed in a spiral shape, closed up and down, Fluid split channels with unequal widths; the fluid split channels of the main body (11) ≥ 3 layers; the first layer of split channels (2) starts from the total inlet (1) processed on the edge of the disc, and ends at the first layer of outer flow channels The outlet (16) ends; a section of drainage embankment (3) is processed on the channel centerline at the beginning of the diversion channel of each layer; the downstream of the diversion dike (3) is processed with a number of diversion island walls (5) with a number ≥ 3, and the diversion The island wall (5) divides the flow channel from the flow opening (4) into an inner flow channel (6) and an outer flow channel (7); each inner flow channel (6) is processed with a protruding swirling wall (9) , a sorting port (8) is next to the downstream of the whirling wall (9); the sorting port (8) is between the two diversion island walls (5), connecting the inner flow channel (6) with the outer flow channel (7) ; The above 1 roundabout impact wall (9) and 2 diversion island walls (5) form a sorting unit; the entrance of the inner flow channel (6) next to the sorting port (8) becomes the diverting port of the next sorting unit (4); the second layer of diversion channel (15) starts from the second layer of drainage dike (3), and ends at the second layer of outer flow channel outlet (14); the third layer of diversion channel (12) from the third layer of drainage The embankment (3) starts and ends at the outlet (13) of the third layer of outer flow channel; the fluid distribution channels of the following layers are analogized in turn, and finally, the inner flow channel (6) ends at the total sample outlet (10) at the center of the main body (11) ; The diversion island wall (5) is fish-shaped, wavy, or diamond-shaped along the direction of the fluid diversion channel; the end of the diversion island wall (5) facing the fluid and opposite to the direction of the fluid is a tapered diversion island wall tip ( 20), the conical tip of the diversion island wall tip (20) is sharpened to form an end face with a width ≥ 1mm, and the end face is processed with a raised irregular convex surface (21) from the side of the inner flow channel (6); The area of the irregular convex surface (21) gradually bifurcates up and down toward the side of the outer flow channel (7) and decreases, and the height also gradually decreases, and finally merges with the irregular concave surface (22) in the middle of the fork of the irregular convex surface (21). In one body; the sidewall surface of the whirling collision wall (9) facing the fluid and opposite to the direction of the fluid is processed with irregular convex surfaces (21) and irregular concave surfaces (22) of different sizes and uneven distribution; The front half of the side of the inner flow channel (6) of the diversion island wall (5) is arc-shaped against the arc of the main body, so that the shape of the inner flow channel (6) is in the second half of the convex surface of the corresponding roundabout wall (9). section, and is in the same direction as the arc of the main body (11); the protruding part of the swinging wall (9) is in the opposite direction of the arc of the main body, and the diversion island wall (5) and the swinging wall (9) Correspondingly, the first half of the inner runner (6) forms an arc-shaped roundabout that is opposite to the arc of the main body, and then forms a channel that is in the same direction as the arc of the main body (11) after passing through the roundabout wall (9). 2. A blood diverter according to claim 1, characterized in that: the drainage dike (3) is a strip with a width of 0.5 mm to 1 mm and a height of 2 mm to 5 mm lower than the top of the fluid diversion channel; The width of the fluid distribution channel in each layer is the same, and the width dimension is ≥ 5mm, or the width of the fluid distribution channel in each layer increases or decreases layer by layer; the height of the distribution island wall (5) is flush with the top of the fluid distribution channel, That is, the fluid distribution channel is closed up and down. 3. A blood shunt according to claim 1, characterized in that: the outer flow channel (7) is an arc-shaped channel with smooth and smooth wall surfaces on both sides and a smooth transition along the arc of the main body (11) in the same direction; The width of the outer channels (7) of the layers is the same, and the width dimension is ≤1/4 of the width of the fluid distribution channel, or the width of the outer channels (7) of each layer gradually decreases or increases. 4. A blood shunt according to claim 1, characterized in that: the widths of the inner channels (6) at the front, middle and rear of a sorting unit are inconsistent, and the inner channels of each layer The width and dimension of the runner (6) are inconsistent. 5. A blood diverter according to claim 1, characterized in that: the total sample inlet (1) and the total sample outlet (10) have the same diameter, and both are larger than the outer channels (7) of each layer The outlet diameter of the outer runner (7) is higher than the height of the bottom (23) of the inner runner (6) by 0.5 mm to 2 mm; the bottom of the outer runner ( 25) The bottom of the inner runner (23) at the sorting port (8) is connected and transitioned by the bottom of the sorting port (24) raised by a circular arc; the bottom of the outer runner (25) is connected and transitioned to a horizontal bottom surface, or After the connection and transition, it is a sloped bottom surface with a high transition point and a low outer side. 6. A blood diverter according to claim 1, characterized in that: the main body (11) can be processed with a dark barrier dike (18) at the diversion port (4) of the sorting unit; the dark barrier dike ( 18) is a dike wall that is transversely penetrated and intercepted in the inner flow channel (6), and its height dimension is 2mm~5mm lower than the top of the inner flow channel (6); the diversion island wall (5) of the main body (11) is A sorting port dark dike (19) can be processed at the mouth (8); the sorting port dark dike (19) is processed to protrude at the end of the diversion island wall (5) facing away from the fluid and in the same direction as the fluid. A section of embankment is transversely intercepted at the sorting port (8), but does not completely pass through the sorting port (8); the height of the hidden embankment (19) at the sorting port is 2mm-5mm lower than the top of the sorting port (8).

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