CN106806374A - A composition for preventing and treating perinatal ruminant ketosis and fatty liver disease - Google Patents

Abstract

The invention discloses a composition for preventing and treating ketosis and fatty liver disease of a ruminant in perinatal period, which comprises rumen-protected glucose and rumen-protected choline and/or rumen-protected nicotinic acid, wherein the ruminant comprises a cow, a beef cattle and a sheep.

Description

A composition for preventing and treating perinatal ruminant ketosis and fatty liver disease

technical field

The invention relates to the field of preventing and treating perinatal ruminant diseases, in particular to a composition for preventing and treating perinatal ruminant ketosis and fatty liver disease.

Background technique

After calving, due to the sharp increase in milk production, the need for energy, especially for glucose, continues to increase, while the rumen-passing carbohydrates provided by the diet for the synthesis of glucose are far from sufficient, resulting in a drop in blood sugar and changes in sugar in the body. The biogenesis mechanism is activated, body protein is used, and body fat is lipolyzed, leading to ketosis or subclinical ketosis and fatty liver disease.

Ketosis is a disorder of sugar and fat metabolism in dairy cows, resulting in the accumulation of large amounts of ketone bodies (including β-hydroxybutyric acid (BHBA), acetoacetate and acetone, mainly β-hydroxybutyric acid) in the blood, which are produced by urine, milk and Exhaled gas is expelled and is characterized by hypoglycemia as a disorder of energy metabolism (Sun Bin et al., 1999; Gruffat D. et al, 1996; Goff JP and Horst RL, 1997; Hocquette JF and Bauchart D, 1999). This disease is a common serious nutritional metabolic disease in dairy cows, and it mostly occurs 10-60 days after calving. Although ketosis can be cured, ketosis will reduce milk production, milk quality, and reproductive rate of dairy cows, and cause reproductive system diseases and endocrine disorders and other diseases, which will increase the cost of treatment and cause serious economic losses to the dairy industry. loss.

Fatty liver disease is a secondary phenomenon of ketosis and a nutritional metabolic disease. After the perinatal glucose metabolism is unbalanced, body fat is lipolyzed to produce a large amount of free fatty acids (non-esterified fatty acids) that cannot be transported to other organs after reaching the liver. The tissues may be oxidized and accumulate in the liver in large quantities, resulting in fatty liver disease.

Choline is an essential nutrient for the body. It is not part of the enzyme system. Choline obtained from the diet of ruminants will be rapidly degraded in the rumen. It has been proven that there may be less than 3% choline, including Unprotected choline (choline chloride) produced by rumen protozoa, obtained in the diet or added alone can enter the small intestine and be absorbed.

Nicotinic acid, also known as nicotinic acid, belongs to the B vitamins and is a general term for pyridine 3-carboxylic acid derivatives with niacin biological activity. In animals, niacin is easily converted to the biologically active nicotinamide, which is a component of two important coenzymes, nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) .

At present, ketosis in dairy cows usually can only be treated by intravenous injection of glucose, which usually needs to be injected continuously for 5 days, which is very inconvenient. However, there is no effective way to prevent this disease. Adding glucose directly to the diet cannot prevent cow ketosis, because glucose directly added to the diet will be destroyed by rumen microorganisms in the rumen and cannot pass through the rumen. Glucose needs to be protected with substances that cannot be degraded in the rumen in order to be effective through the rumen.

The above-mentioned ketosis and fatty liver disease can be alleviated by introducing rumen-passing protection technology (referred to as rumen-passing technology), specifically using physical or chemical methods to protect some nutrients that are easily destroyed by rumen microorganisms from being destroyed by rumen microorganisms Decompose, reach the abomasum and intestinal tract, disintegrate and release, and be used by ruminants.

The information disclosed in this Background section is only for enhancing the understanding of the general background of the present invention and should not be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art.

Contents of the invention

The object of the present invention is to provide a composition with higher biological potency for preventing and treating perinatal ruminant ketosis and fatty liver disease, thereby more effectively improving the prevention and treatment of perinatal ruminant ketosis and fatty liver disease. The therapeutic effect can reduce the occurrence of postpartum weightlessness, increase the conception rate during estrus, and increase milk production.

To achieve the above object, the present invention provides a composition for preventing and treating ketosis in ruminants, the composition includes rumen-passing protected glucose, rumen-passing protected choline and/or rumen-passing protected niacin.

The present invention also provides a composition for preventing and treating ketosis in ruminants, wherein the rumen-protected niacin is replaced by rumen-protected nicotinamide.

Preferably, in the above technical scheme, the mass percentage of glucose contained in the rumen-passing protection glucose is more than 35%, the mass percentage of choline contained in the rumen-passing protection choline is more than 20%, and the rumen-passing protection niacin The mass percentage of the niacin contained in it is more than 35%.

Preferably, in the above technical solution, the ruminants include dairy cows, beef cattle and sheep.

Preferably, in the above technical solution, the rumen-passing protected choline includes rumen-passing protected choline chloride.

Preferably, in the above technical solution, the mass ratio of the rumen-passed glucose to the rumen-protected choline is 150-600:10-100.

Preferably, in the above technical solution, the mass ratio of the rumen-passing protected glucose to rumen-passed niacin is 150-600:2-20.

Preferably, in the above technical solution, the mass ratio of the rumen-protected glucose, rumen-protected choline and rumen-protected niacin is 150-600:10-100:2-20.

Preferably, in the above technical solution, the mass ratio of the rumen-protected glucose, rumen-protected choline and rumen-protected niacin is 180-500:20-90:4-15.

The present invention also provides a composition for preventing and treating ketosis in ruminants, wherein the rumen-passing protected glucose is replaced by rumen-passing protected disaccharides and/or polysaccharides, wherein the disaccharides are sucrose other than brown sugar, Maltose, lactose, etc., polysaccharides are starch, etc.

Compared with the prior art, the present invention has the following beneficial effects: the combination of these three rumen-passing protective substances combines the metabolic mechanism of dairy cows during the perinatal period, and the main nutritional factors that affect dairy cow fatty liver disease and ketosis: glucose, choline, tobacco It has been treated with rumen protection to increase its biological potency; it has improved the effect of prevention and treatment of peripartum ketosis and fatty liver disease in dairy cows, reduced the incidence of ketosis in dairy cows by more than 50%, and reduced the incidence of fatty liver disease in dairy cows by 30% % or more; reduce the occurrence of postpartum weightlessness; increase the reproductive rate by more than 40%; and can increase the milk production of high-yield dairy cows by 3-5kg, the highest increase to more than 8kg, increase the milk protein rate by 0.2 percentage points, and increase the milk fat rate by 0.2-0.4 percentage points , the effect is remarkable, and it is of great significance to the healthy and sustainable development of my country's dairy industry.

Specifically, by using the composition defined in this application, products such as acetone, acetoacetate and β-hydroxybutyric acid are no longer produced in the liver of ruminants and enter the blood, while the original acetone, acetoacetate and β-hydroxybutyrate in the blood Butyric acid, etc. are also rapidly metabolized in the body, and ketosis is no longer produced.

The glucose in the composition can effectively pass through the rumen after being protected by the rumen, blocking the mechanism of sugar heterogenesis caused by the imbalance of glucose metabolism in the perinatal period, so that the body protein is no longer used, and the body fat is no longer lipolysis; niacin passes through the rumen After protection, it enters the body tissue. Because niacin has anti-lipolysis effect, it has a stronger anti-lipolysis effect when used in conjunction with rumen-passing glucose protection, so that body fat will no longer be used, and a large amount of free fatty acids will not be produced. In this way, fatty acids deposited in liver tissue No more increase; choline enters the liver after being protected by the rumen, synthesizes phosphatidylcholine, and forms very low-density lipoprotein with triglycerides, transports the fat in the liver out of the liver, and uses it in various tissues, and fatty liver disease will no longer occur .

And, when the composition defined in the application is used, after the gluconeogenesis of protein in ruminants is blocked, the degradation of body protein and body fat will be improved, thereby improving the mobilization of body protein and body fat. The lipolysis leads to postpartum weight loss, and the body weight gradually returns to normal.

Furthermore, after protein gluconeogenesis is prevented, it improves the process of protein gluconeogenesis, due to the large amount of ammonia produced by the transamination of amino acids, which leads to the increase of blood ammonia concentration and the production of a large amount of urea nitrogen. rate of decrease. After adding rumen-protected choline, 30% of the methionine in the body is not used to synthesize choline, but a large amount is used to synthesize milk protein, and the milk production will increase.

detailed description

Specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

The rumen-passing products used in the following compositions are produced by Beijing Yahe Nutrition High-tech Co., Ltd., the rumen-passing rate is not less than 90%, and the small intestine release rate is not less than 90%, among which the rumen-passing protects the quality of glucose contained in the glucose The percentage is 35%, the mass percentage of the choline chloride contained in the rumen-passed protected choline chloride is 20%, and the mass percentage of the niacin contained in the rumen-passed niacin is 35%.

combination

Composition 1: 400 grams of rumen-protected glucose, 25 grams of rumen-protected choline chloride and 5 grams of rumen-protected niacin.

Composition 2: 300 grams of rumen-protected glucose, 60 grams of rumen-protected choline chloride and 5 grams of rumen-protected niacin.

Composition 3: 200 grams of rumen-protected glucose, 80 grams of rumen-protected choline chloride and 8 grams of rumen-protected niacin constitute the composition.

Implementation

1. Experimental animals and grouping

In the intensive 1,000-head pasture, according to the principle of similar age, body condition, and parity, 28 healthy dairy cows with similar expected delivery dates were selected, including 21 cows in the experimental group, divided into groups I, II, and III, with 7 cows in each group. There were 7 cows in the control group; the cows in the experimental group and the control group were fed normally according to the NRC feeding standard before and after delivery.

2. Specific feeding situation:

Group I, Group II, and Group III cows were fed with the above-mentioned composition 1, composition 2, and composition 3 respectively. Specifically, the cows in the experimental group were fed with the above-mentioned corresponding composition every day at the same time point from 0d to 21 days after delivery. The doses were 430 g/head·day, 365 g/head·day, and 288 g/head·day respectively, and the cows in the control group did not receive any treatment.

3. Sample collection

3.1 Lactation performance

The experimental group and the control group recorded the average milk production and milk index at 14d, 21d and 45d after delivery respectively.

3.2 Blood indicators

In the experimental group and the control group, 10ml of tail root venous blood was collected 1 week before delivery, on the day of calving, 1 week after delivery, and 2 weeks after delivery, and centrifuged at room temperature 3000rpm/min for 10min to separate serum, which was frozen at -20°C for testing. Among them, 0d, 1d, and 2d are fed after blood collection.

4. Index detection:

Milk Indicators: Using a Dairy Analyzer

Blood biochemistry: Use a semi-automatic biochemical analyzer to detect the concentrations of β-hydroxybutyric acid (BHBA), free fatty acids (NEFA), blood glucose (GLU), and blood calcium (Ga).

5. Data processing:

The spss19.0 data processing software was used for data analysis, and the results were expressed as "mean ± standard deviation"; and the incidence of ketosis in 28 experimental cattle within 7 days, 14 days and 21 days after delivery was counted.

6. Experimental results

The experimental results of Group II and Group III are not much different, and the experimental results of Group I and Group II are listed as follows:

The impact on the production performance of dairy cows is shown in Table 1:

Table 1 Effects on the performance of dairy cows

There is no letter in the same column or the same letter on the data shoulder indicates no significant difference (P>0.05), different lowercase letters indicate significant difference (P<0.05), different uppercase letters indicate extremely significant difference (P<0.01). The table below is the same.

The impact on the blood biochemical indicators of dairy cows is shown in Table 2

Table 2 Effect of RPC on blood biochemical indexes of dairy cows

Table 3 Test results of postpartum ketone bodies in dairy cows

From the analysis results of the above indicators, it can be seen that the important indicators of BHBA, NEFA, and GLU to measure ketosis in the data analysis show differences to varying degrees, and the incidence rates of different groups of postpartum ketone body test results of dairy cows are also different. This shows that the composition of the present invention has preventive and therapeutic effects on dairy cow ketosis and fatty liver disease, and the composition of the present invention has a rapid regulation effect on blood sugar, blood calcium, and other indicators, maintains blood sugar concentration, and can reduce the incidence of postpartum ketosis , to ensure the normal function of the body; the composition can also control blood BHBA and NEFA concentrations, and effectively control the negative energy metabolism state of dairy cows for a long time; and after adding the composition of the present invention, compared with the control group, the milk production, milk fat Rate, milk protein rate, lactose rate have different degrees of improvement compared with the control group, thereby increasing the milk production of cows to a certain extent, and improving milk production performance.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling others skilled in the art to make and use various exemplary embodiments of the invention, as well as various Choose and change. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (10)

1. it is a kind of prevent and treat ruminant ketoacidosis composition, the composition include rumen-protected glucose, also wrap Include rumen-protected choline and/or rumen-protected nicotinic acid.

2. it is according to claim 1 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the rumen bypass Protection nicotinic acid is replaced with rumen-protected niacinamide.

3. it is according to claim 1 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the rumen bypass Protection glucose included in glucose quality percentage more than 35%, the courage included in the rumen-protected choline The mass percent of alkali more than 20%, the mass percent of the nicotinic acid included in the rumen-protected nicotinic acid for 35% with On.

4. the composition of prevention and treatment ruminant ketoacidosis according to claim 1 and 2, it is characterised in that described anti- Hay animal includes milk cow, beef cattle and sheep.

5. it is according to claim 1 and 2 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the mistake Ruminally-protected choline includes rumen-protected Choline Chloride.

6. it is according to claim 1 and 2 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the mistake The mass ratio of ruminally-protected glucose and rumen-protected choline is 150-600:10-100.

7. it is according to claim 1 and 2 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the mistake The mass ratio of ruminally-protected glucose and rumen-protected nicotinic acid is 150-600:2-20.

8. it is according to claim 1 and 2 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the mistake The mass ratio of ruminally-protected glucose, rumen-protected choline and rumen-protected nicotinic acid is 150-600:10-100:2-20.

9. it is according to claim 1 and 2 prevention and treatment ruminant ketoacidosis composition, it is characterised in that the mistake The mass ratio of ruminally-protected glucose, rumen-protected choline and rumen-protected nicotinic acid is 180-500:20-90:4-15.

10. it is according to claim 1 prevention and treatment ruminant ketoacidosis composition, it is characterised in that it is described cross knurl Stomach protection glucose is replaced with rumen-protected disaccharide and/or polysaccharide.