CN106806039A - Rotary cell support planting unit - Google Patents

Abstract

The invention provides a rotary cell scaffold planting device, which includes a device body and a host computer interface. The device body is provided with a core board and an interface board to form a microprocessor; the microprocessor is connected to a temperature sensor and a heating rod through a solid-state relay to realize constant temperature control; the microprocessor controls the _CO2 sensor and the _CO2 gas circuit, The ultraviolet lamp is connected to the motor through the driver, and a co-cultivation mold is fixed on the shaft of the motor, and the cell culture solution and the intravascular stent are placed in the mold to provide a sterile planting environment. The host computer interface is connected to the microprocessor through a serial port, and the host computer interface, infrared remote control and liquid crystal display complete the real-time monitoring and control, data storage and display of temperature, rotation speed and CO ₂ . The invention solves the problems of uneven cell growth distribution and weak adhesion on the intravascular stent, is easy to use, can work independently without using a _CO2 constant temperature incubator, and realizes the planting function of the cell stent, and has application and clinical value.

Description

Rotary Cell Scaffold Planting Device

【Technical field】

The invention relates to the fields of medical equipment and tissue engineering, and in particular proposes a rotating cell scaffold planting device for simulating three-dimensional cell culture.

【Background technique】

In the treatment of coronary artery stenosis and cardiovascular diseases, intravascular stents play an important role. The current research on intravascular stents mainly focuses on the drug-loaded and degradable aspects of the stent, but the ultimate goal is for the endothelialization process during treatment. Studies have found that in the treatment of vascular diseases, in vitro cell-planted scaffolds are a more direct method of endothelialization. Under the same conditions, endothelial cell-planted scaffolds can achieve better histocompatibility and blood compatibility, and more It is beneficial to the treatment of cardiovascular diseases, combined with the application of cell culture engineering and cell scaffold planting technology.

Cell stent planting technology is to use intravascular stent as a cell carrier, spray a layer of medium on its surface, plant genetically modified cells on the surface of the stent, and implant it into the body to achieve the purpose of cell inoculation, so as to treat atherosclerosis Damaged endothelium in sclerosis, etc. The cell scaffold planting scaffold drives the research and development of the cell scaffold planting device.

However, due to the effect of gravity, the conventional in vitro static culture method is almost impossible to establish a local three-dimensional microenvironment for tissue cells in the body, and cannot provide environmental conditions for normal growth and development of tissue cells. The cells lose their original three-dimensional shape, resulting in scaffolding problems such as poor cell adhesion. The three-dimensional cell culture technology has promoted the development of tissue engineering. The rotating cell culture system simulates the microgravity environment through the continuous rotation of the reactor, creating a new research field for it.

Although the existing cell scaffold planting device can produce cell scaffolds that meet certain requirements, the problems exposed in the experimental research and use are as follows: 1. The local scaffolds have uneven growth, poor adhesion, and cell shedding; 2. The device needs to provide corresponding culture conditions with the help of a CO ₂ constant temperature incubator; 3. Cell scaffolds cannot be produced in batches; 4. The culture environment cannot be dynamically monitored, and there is no alarm system when the culture conditions are out of balance; 5. The degree of automation of the equipment is low, It is cumbersome to use.

【Invention patent content】

Aiming at the defects in the prior art, the present invention provides a rotary cell scaffold planting device.

The technical scheme of the present invention is as follows: a rotary cell scaffold planting device includes a device body (1) and a host computer interface (2). The device body is provided with a core board and an interface board to form a microprocessor; the microprocessor is connected to a temperature sensor and a heating rod through a solid state relay to realize constant temperature control; the microprocessor controls the _CO2 sensor and the _CO2 gas circuit, ultraviolet The lamp is connected to the motor through the driver, and the co-cultivation mold is fixedly connected to the shaft of the motor, and the cell culture solution and the intravascular stent are placed in the mold to provide a sterile planting environment. The host computer interface is connected to the microprocessor through a serial port, and the host computer interface, infrared remote control and liquid crystal display complete the real-time monitoring and control, data storage and display of temperature, rotation speed and CO ₂ . The invention solves the problems of uneven cell growth distribution and weak adhesion on the intravascular stent, is easy to use, can work independently without using a CO2 constant temperature incubator, and realizes the planting function of the cell stent, and has application and clinical value.

The device is equipped with a blower to make the temperature of the cultivation chamber more uniform. The temperature control range of the cultivation chamber is 37.0±0.1°C. After constant temperature, the temperature fluctuation of the cultivation chamber is ≤0.1°C, and the _CO2 concentration is 5%.

The motor is a stepping motor, and the rotation speeds of forward rotation and reverse rotation are continuously adjustable, and the rotation speed ranges from 0 to 5 revolutions per minute.

The microprocessor is provided with an abnormal alarm device for temperature and _CO2 concentration.

The inner diameter of the co-cultivation mold is 60mm, and there are two symmetrical branch pipes on the mold, with a diameter of 4mm, which is convenient for the replacement of the cell culture solution. One end of the co-cultivation mold is covered with an air-permeable and liquid-impermeable semi-permeable membrane, which is convenient for gas exchange; The culture mold has multiple scaffold fixing points, which can realize the batch preparation of cell scaffolds.

The device can be used through red and infrared remote control or a host computer.

The beneficial effects of the present invention are: the device realizes automatic control, and is easy to use; it can perform independent operations without resorting to a _CO2 constant temperature incubator; Find problems at the first time to avoid the occurrence of experimental misoperation; can provide a stable co-culture environment, so that cells can grow uniformly and firmly on the intravascular scaffold; multiple scaffold fixing points can realize batch preparation of cell scaffolds, and the planting efficiency is high ,save time.

【Description of drawings】

Fig. 1 is a schematic structural view of a rotary cell scaffold planting device;

Fig. 2 is a schematic diagram of the co-cultivation mold structure;

Figure 3 is a schematic diagram of the structure of the CO2 gas path;

Figure 4 is a functional diagram of the host computer.

In the figure: 1. device body; 9. cultivation chamber; 151. mold body; 152. mold cover; 153. fixed connector; 154. bracket fixing point; . Holes.

【detailed description】

Described below is the specific implementation manner of the patent of the present invention.

As shown in FIG. 1 , the rotary cell scaffold planting device mainly includes a device body 1 and a host computer 2 . The device body 1 is provided with a core board 3 and an interface board 4, which constitute a microprocessor, and a switch group, including a device power switch, an ultraviolet light switch, a blower switch, and a temperature control, gas Control and speed control switch. The microprocessor is connected to the temperature sensor 5 and the heating rod 7 through the solid state relay 6. The blower 8 in the device body 1 stirs to make the temperature of the cultivation chamber 9 more uniform, and realizes the constant temperature control of the cultivation chamber 9. The temperature control range: 37.0±0.1°C, After constant temperature, the temperature fluctuation is less than or equal to 0.1°C; the microprocessor controls the CO2 sensor 10, the CO2 gas circuit 11, the ultraviolet lamp 12, and the stepper motor 14 through the driver 13, and the co-culture mold 15 is fixedly connected to the motor shaft, and the cells are placed in the mold The culture medium and intravascular stent, the forward and reverse rotation speed of the motor are continuously adjustable, the speed range is 0-5 rpm, and the _CO2 concentration is controlled at 5%. Microprocessor built-in PID control algorithm and alarm system, if the temperature or CO ₂ gas path is abnormal, it will alarm. The device body 1 is provided with an infrared receiving head 16 and a liquid crystal display 17 for performing parameter setting and parameter display functions of the infrared remote controller.

As shown in Figure 2, the host computer 2 communicates through the serial port 18, not only can set the cultivation environment parameters shown in the figure, but also can monitor, display and store them synchronously. In addition, when the device is in use, only the infrared remote controller can be used to realize some related operations of the upper computer.

As shown in Figure 3, the co-cultivation mold 15 is one of the core components of the device body, with an inner cavity diameter of 60mm, built-in cell culture fluid and intravascular stent, and consists of three parts: a mold body 151, a mold cover 152, and a fixed connector 153 , a plurality of vascular stent fixing points 154 are evenly arranged at the bottom of the mold body 151, a toothpick of an appropriate length is inserted, and the stent can be fixed by hanging the stent cover on the toothpick, which can realize batch preparation of cell scaffolds; two symmetrical branch pipes 155 are symmetrically opened on the side , diameter 4mm, easy to replace the cell culture medium. The mold cover is provided with a plurality of small grooves 156, and the left side of the mold cover is covered with an air-permeable and liquid-tight semi-permeable membrane, which is convenient for gas exchange. Fix the mold body 151 and the mold cover 152 with screws through the screw hole 157 with the fixed connector 153 to prevent the liquid from leaking out.

As shown in Figure 4, the CO2 gas is composed of a gas storage cylinder, a speed regulating valve, a solenoid valve group, a throttle valve, a filter, and a microprocessor. The microprocessor controls the speed regulating valve, solenoid valve 1, and filter to make CO2 gas fill the cultivation chamber first, then adjust the speed regulating valve, open the solenoid valve 2 and the throttle valve, and realize a small amount of gas supplementation.

24 hours before planting, press the UV light switch to sterilize the box, and turn on the gas control switch 10 minutes before planting to fill the cultivation room with CO2 gas. When planting, after fixing the co-cultivation mold, press the power switch and the blower switch to power on the equipment, set the temperature and rotation related parameters, press the temperature control and speed control switches, and the upper computer and LCD display the cultivation conditions at the same time. The controller is equipped with PID control algorithm and alarm system. During this period, if the temperature or gas path is abnormal, it will alarm. When the planting is completed, turn off the upper computer or the infrared remote control switch group and the device body switch group in turn.

It should be noted that the present invention is also applicable to cell implantation on artificial blood vessels. The above implementations are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art Personnel should understand that the technical solution of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solution of the present invention, and all of them should be covered by the claims of the present invention.

Claims (8)

1. A rotary cell scaffold planting device, characterized in that: comprising a device body (1) and a host computer (2), the device body (1) is provided with a core board (3) and an interface board (4), constitute a microprocessor. 2. The rotary cell scaffold planting device according to claim 1, characterized in that: the microprocessor is connected to the temperature sensor (5) and the heating rod (7) through a solid state relay (6) respectively, and the device body (1) There is a blower (8) inside, stirring to make the temperature of the cultivation chamber (9) more uniform, to achieve constant temperature control, the temperature control range of the cultivation chamber (9): 37.0±0.1°C, after constant temperature, the cultivation chamber (9) The temperature fluctuation is ≤0.1℃. 3. The rotary cell scaffold planting device according to claim 1, characterized in that: the microprocessor controls the _CO2 sensor (10) and the _CO2 gas path (11), the ultraviolet lamp (12) and the driver (13) and The motor (14) is connected, and a co-cultivation mold (15) is fixed on the motor machine shaft. Cell culture fluid and intravascular stents are placed in the mold to provide a sterile planting environment, and the _CO concentration is controlled to remain at 5%. 4. according to the described rotary cell scaffold planting device of claim 1, it is characterized in that: described device body (1) is provided with infrared receiving head (16), utilizes the setting of relevant parameter of infrared remote control, described device body ( 1) A liquid crystal display (17) is provided to realize the parameter display function. 5. according to the described rotary cell scaffold planting device of claim 1, it is characterized in that: host computer interface (2) communicates with device body (1) through serial port (18), realizes temperature, rotating speed, CO ₂ related parameters Setting, real-time monitoring, data storage and display. 6. The rotary cell scaffold planting device according to claim 2, characterized in that: the microprocessor is provided with a temperature and _CO2 abnormal alarm device. 7 . The rotary cell scaffold planting device according to claim 2 , characterized in that: the forward and reverse speeds of the motor ( 12 ) are continuously adjustable, and the rotational speed ranges from 0 to 5 revolutions per minute. 8. The rotary cell scaffold planting device according to claim 2, characterized in that: the inner diameter of the co-cultivation mold (15) is 60mm, and there are two symmetrical branch pipes on the mold with a diameter of 4mm, which is convenient for the replacement of the cell culture solution , One end of the co-culture mold is covered with a gas-permeable and liquid-impermeable semi-permeable membrane, which is convenient for gas exchange; the co-culture mold has multiple fixation points for intravascular scaffolds, which can realize the batch preparation of cell scaffolds.