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CN106804627B - A kind of compound microorganism wettable powder pesticide and its preparation method and use - Google Patents

A kind of compound microorganism wettable powder pesticide and its preparation method and use Download PDF

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Publication number
CN106804627B
CN106804627B CN201710067939.3A CN201710067939A CN106804627B CN 106804627 B CN106804627 B CN 106804627B CN 201710067939 A CN201710067939 A CN 201710067939A CN 106804627 B CN106804627 B CN 106804627B
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streptomyces
powder
bacillus
vitamins
stabilizer
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CN106804627A (en
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施李鸣
李嵘
李峥
王金子
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Beijing Kingdom Biotechnology Co Ltd
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Beijing Kingdom Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10—Animals; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A01N25/14—Powders or granules wettable

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of complex microorganism Wettable pesticide powders, it is made of the component of following mass percent: golden yellow vertical streptomycete fermentation object 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, choline chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, microelement 0.5%~2%, wetting agent 2%~5%, dispersing agent 2%~5%, stabilizer 2%~5%, filler is supplied to 100%.Complex microorganism Wettable pesticide powder composition collocation science of the invention, compatibility are reasonable；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, control cost is effectively reduced；Environmental pollution is small, has nuisanceless and the characteristics of without residual hazard, effectively prevents the harm of chemical pesticide.In addition, the invention discloses the Preparation method and uses of the complex microorganism Wettable pesticide powder.

Description

A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes
Technical field
The present invention relates to a kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes, belong to pesticide neck Domain.
Background technique
The domestic prevention and treatment for plant especially corps diseases is substantially using chemical pesticide at present, in chemical pesticide In the case that drawback is increasingly prominent, some microbial pesticides gradually receive the highest attention of domestic and foreign scholars.Microbial source The exploitation and application of pesticide have been increasingly becoming the new trend of pesticide industry, and main advantage is: 1) many kinds of, the choosing of research and development It is big to select leeway；2) specificity is strong, to non-target organism safety, is not likely to produce drug resistance；3) activity is high, and production cost is low, fermentation Simple process；4) environmentally safe, nuisanceless, noresidue.
But the pesticide of existing microbial source can be only applied to the prevention and control of a kind of or a kind of disease, application limitation is very Greatly, peasant also wishes that a kind of pesticide as much as possible can be suitable for a variety of diseases, rather than a kind of pesticide can be only applied to one Kind or a kind of disease, therefore, develop a kind of safety, low toxicity, stablize, efficiently, the microbial pesticide of has a broad antifungal spectrum compels in eyebrow Eyelash.
Summary of the invention
It is an object of the invention to develop a kind of complex microorganism Wettable pesticide powder, the microbial pesticide release Rate is high, and has a broad antifungal spectrum can efficiently prevent and cure diseases, and safe, low toxicity, the nutriment containing there are many, is conducive to crop yield, can grow Phase, which stablizes, to be saved.
To achieve the above object, the technical scheme adopted by the invention is as follows: a kind of complex microorganism Wettable pesticide powder, It is made of the component of following mass percent: golden yellow vertical streptomycete fermentation object 10%~15%, rose yellow streptomycete mycelia 10%~15%, actinomyces styreptomyces globispotus strain original powder 10%~15%, Wuyi bacterium conidia powder 10%~15%, bacillus mycelia 10%~15%, choline chloride 0.5%~2%, light rare earth 0.5%~2%, vitamins 0.5%~2%, microelement 0.5%~2%, wetting agent 2%~5%, dispersing agent 2%~5%, stabilizer 2%~5%, filler are supplied to 100%.
Preferably, the deposit number of golden yellow vertical streptomycete is CCTCC NO.M2010293, and the preservation of rose yellow streptomycete is compiled It number is CGMCC NO.1471, the deposit number of actinomyces styreptomyces globispotus strain is CCTCC NO.M 2011012, and bacillus is ground Clothing bacillus, bacillus subtilis or wax bacillus.
Wherein choline chloride, light rare earth, vitamin etc. are conducive to plant stress-resistance growth promoting, have apparent disease resistance, resist It is inverse, improve crop yield, improve crop product quality effect, this formula under microbial pesticide in Microbiological release Rate is high, has a broad antifungal spectrum, and safe and environment-friendly.
Preferably, the golden yellow vertical streptomycete fermentation object is prepared by following steps: 1) activate golden yellow vertical streptomycete: Golden yellow vertical streptomycete is seeded on Gause I culture medium flat plate with method of scoring, 28 DEG C constant temperature incubation 4 days, the matter of culture medium Measure percentage composition: soluble starch 2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete of activation is inoculated in level-one kind In sub- culture solution, 180rmin-1, 28 DEG C of culture 72h, the mass percentage composition of first order seed culture solution: soluble starch 2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, moisturizing is extremely 100%；3) the first order seed culture solution prepared is seeded in secondary seed culture solution, 180rmin-1, 28 DEG C of cultures 72h, centrifuge separation obtain golden yellow vertical streptomycete fermentation object, low temperature drying, and moisture content is lower than 5%, secondary seed culture solution Mass percentage composition: glucose 0.05%, soluble starch 0.05%, K2HPO40.05%, NaCl 0.05%, MgSO4 0.05%, peptone 0.05%, yeast extract 0.05% and soybean powder 0.05%, moisturizing to 100%.
Preferably, the rose yellow streptomycete mycelia is prepared by following steps: 1) being cultivated rose yellow streptomycete in PDA It is cultivated 5 days for 28 DEG C on base；2) it is then inoculated into seed culture medium with 2% ratio, condition of culture are as follows: 28 DEG C, 180r min-1, cultivate 3 days, wherein seed culture medium are as follows: and 3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream, 0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% sulfuric acid Zinc, 3.5% sucrose, 0.4% fructose；3) the rose yellow streptomycete culture that step (2) is prepared is by being obtained by filtration State rose yellow streptomycete mycelia.
Preferably, the actinomyces styreptomyces globispotus strain original powder is prepared by following steps: 1) by activated actinomyces Styreptomyces globispotus strain is inoculated into 2% ratio is inoculated in Gause I fluid nutrient medium, and 28 DEG C, 150-160rmin-1Oscillation training Actinomyces seed culture fluid is obtained after supporting 120h；2) the actinomyces styreptomyces globispotus strain that step (2) is prepared is by filtering, doing It is dry to obtain the actinomyces styreptomyces globispotus strain original powder.
Preferably, the bacillus mycelia is prepared by following steps: activated bacillus is pressed to 2% ratio Example is inoculated in culture medium, is cultivated 5 days at 28 DEG C, is taken out culture and is filtered, obtained solid is bacillus bacterium Silk；Wherein the bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
Preferably, the complex microorganism Wettable pesticide powder is made of the component of following mass percent: golden yellow to hang down Straight chain mold fermentation object 10%~12%, rose yellow streptomycete mycelia 10%~12%, actinomyces styreptomyces globispotus strain original powder 10% ~12%, Wuyi bacterium conidia powder 10%~12%, bacillus mycelia 10%~12%, choline chloride 0.5%~1%, light dilute Soil 0.5%~1%, vitamins 0.5%~1%, microelement 0.5%~1%, wetting agent 2%~4%, dispersing agent 2%~ 4%, stabilizer 2%~4%, filler are supplied to 100%.This formula under pesticide wetability, dispersibility, suspension, granulating rate, High stability, and preventing disease theraping effect is preferable.
It is furthermore preferred that the complex microorganism Wettable pesticide powder is made of the component of following mass percent: golden yellow Vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium spore Powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, microelement 1%, profit Humectant 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%.This is formulated lower complex microorganism Wettable pesticide powder Wetability, dispersibility, suspension, granulating rate, stability be all up optimum level, after measured, complex microorganism wettable powder It can be stored 6 months under the shelf storage life room temperature of agent pesticide, bacteriostasis rate is positively retained at 85% or more, and preventing disease theraping effect is most It is excellent.
Preferably, the vitamins is at least one of vitamin, B or C.It is furthermore preferred that the vitamins is The mixture of vitamin B and C, the mass ratio of the two are 1:1.
Preferably, the microelement is B, Mn, Mo, Cu, Zn and Fe.Preferred described B, Mn, Mo, Cu, Zn and Fe Mass ratio be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, sodium molybdate, sulphur Sour copper, zinc sulfate, ferrous sulfate.When using the microelement of the quality proportioning, the complex microorganism wettable powder Pesticide performs to utmostly the production-increasing function of crops.
Preferably, the wetting agent is lauryl sodium sulfate, Tween-80, at least one in sodium methylene bis-naphthalene sulfonate Kind；The dispersing agent is at least one of sodium lignin sulfonate, sodium lignin sulfonate calcium, poly-aspartic-acid；The stabilization Agent is at least one of vitamin E, potassium dihydrogen phosphate；The filler is diatomite, bentonite, at least one in kaolin Kind.
It is furthermore preferred that the wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, wherein the matter of the two Amount is than being 1:1；The dispersing agent is sodium lignin sulfonate calcium；The stabilizer is vitamin E；The filler is kaolin and silicon The mixture of diatomaceous earth, wherein the mass ratio of the two is 1:2.
Preferably, a kind of complex microorganism Wettable pesticide powder of the present invention, by the group of following mass percent Divide and be made: golden yellow vertical streptomycete fermentation object 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder 15%, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, choline chloride 1%, light rare earth 2%, vitamins 2%, micro Element 1%, wetting agent 4%, dispersing agent 4%, stabilizer 4%, filler are supplied to 100%；Wherein vitamins be vitamin B and The mixture of C, the mass ratio of the two are 1:1；Microelement is B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, Zn and Fe Mass ratio be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, sodium molybdate, sulphur Sour copper, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Dispersing agent is poly-aspartic-acid；Stabilizer is dimension Mixture in raw element E and potassium dihydrogen phosphate, wherein the mass ratio of the two is 1:1；Filler is bentonite and kaolinic mixing Object, wherein the mass ratio of the two is 1:1.
It is furthermore preferred that component system of the complex microorganism Wettable pesticide powder of the present invention by following mass percent At: golden yellow vertical streptomycete fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, force Smooth bacterium conidia powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, micro member Element 1%, wetting agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%；Wherein the vitamins is vitamin B With the mixture of C, the mass ratio of the two is 1:1:1；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, The mass ratio of Cu, Zn and Fe be 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, Sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, Both middle mass ratio is 1:1；Dispersing agent is calcium lignosulfonate；Stabilizer is vitamin E；The filler is kaolin and silicon The mixture of diatomaceous earth, wherein the mass ratio of the two is 1:2.
In addition, the present invention discloses a kind of preparation method of complex microorganism Wettable pesticide powder described above, packet It includes following steps: wetting agent, dispersing agent, stabilizer, filler, choline chloride, light rare earth being put into air-flow according to the ratio It is crushed in pulverizer, crosses 350 meshes, after cooling, golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, unwrapping wire is added Bacterium styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder, bacillus mycelia, vitamins, microelement, continue to crush, obtain institute State complex microorganism Wettable pesticide powder.
In addition, the present invention discloses one kind complex microorganism Wettable pesticide powder described above withered in prevention and treatment banana Purposes in disease, winter jujube rust, cotton verticillium wilt and peanut yield increasing.
The beneficial effects of the present invention are: complex microorganism Wettable pesticide powder composition collocation science of the invention is matched 5 rationally；Has a broad antifungal spectrum, activity is high, all has good preventing disease theraping effect to many crop diseases, be effectively reduced prevention and treatment at This；Lower production costs favorably improve the competitiveness of product in market；Environmental pollution is small, there is spy nuisanceless and without residual hazard Point effectively prevents the harm of chemical pesticide.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention It is described further.
Embodiment 1
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain Mold fermentation object 10%, rose yellow streptomycete mycelia 15%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder 15%, bacillus mycelia 10%, choline chloride 2%, light rare earth 0.5%, vitamins 0.5%, microelement 2%, wetting Agent 2%, dispersing agent 5%, stabilizer 5%, filler are supplied to 100%.
Wherein, the vitamins is vitamin C；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, The mass ratio of Mo, Cu, Zn and Fe are 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, sulfuric acid Manganese, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is lauryl sodium sulfate；The dispersing agent is sulfomethylated lignin Sour sodium；The stabilizer is vitamin E；The filler is diatomite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using following methods: according to above-mentioned recipe ratio Wetting agent, dispersing agent, stabilizer, filler, choline chloride, light rare earth are put into airslide disintegrating mill and are crushed by example, cross 350 mesh After cooling, golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi is added in sieve Bacterium conidia powder, bacillus mycelia, vitamins, microelement continue to crush to get the complex microorganism wettable powder is arrived Agent pesticide；
Wherein, golden yellow vertical streptomycete fermentation object is prepared by following steps: 1) activating golden yellow vertical streptomycete: to cross Golden yellow vertical streptomycete is seeded on Gause I culture medium flat plate by method, 28 DEG C constant temperature incubation 4 days, the quality percentage of culture medium Than composition: soluble starch 2.5%, KNO30.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, agar 2.5%, moisturizing to 100%；2) the golden yellow vertical streptomycete of activation is inoculated in first order seed culture In liquid, 180rmin-1, 28 DEG C of culture 72h, the mass percentage composition of first order seed culture solution: soluble starch 2.5%, KNO3 0.2%, K2HPO40.05%, MgSO40.05%, NaCl 0.05%, FeSO40.05%, moisturizing to 100%；It 3) will preparation Good first order seed culture solution is seeded in secondary seed culture solution, 180rmin-1, 28 DEG C of culture 72h, centrifuge separation, obtain Golden yellow vertical streptomycete fermentation object, low temperature drying, moisture content are lower than 5%, the mass percentage composition of secondary seed culture solution: Glucose 0.05%, soluble starch 0.05%, K2HPO40.05%, NaCl 0.05%, MgSO40.05%, peptone 0.05%, yeast extract 0.05% and soybean powder 0.05%, moisturizing to 100%.
Wherein, rose yellow streptomycete mycelia is prepared by following steps: 1) by rose yellow streptomycete in PDA culture medium 28 DEG C culture 5 days；2) it is then inoculated into seed culture medium with 2% ratio, condition of culture are as follows: 28 DEG C, 180rmin-1, culture 3 days, wherein seed culture medium are as follows: 3.5% groundnut meal, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother's cream, 0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose, 0.4% fructose；3) the rose yellow streptomycete culture that step (2) is prepared is by being obtained by filtration the rose yellow streptomycete Mycelia.
Wherein, actinomyces styreptomyces globispotus strain original powder is prepared by following steps: 1) by activated actinomyces ball spore chain Mould is inoculated into 2% ratio is inoculated in Gause I fluid nutrient medium, and 28 DEG C, 150-160rmin-1Shaken cultivation 120h Actinomyces seed culture fluid is obtained afterwards；2) the actinomyces styreptomyces globispotus strain that step (2) is prepared passed through into filtering, be dried to obtain The actinomyces styreptomyces globispotus strain original powder.
Wherein, bacillus mycelia is prepared by following steps: activated bacillus is inoculated in 2% ratio It in culture medium, is cultivated 5 days at 28 DEG C, takes out culture and be filtered, obtained solid is bacillus mycelia；Wherein The bacillus is bacillus licheniformis, bacillus subtilis or wax bacillus.
Embodiment 2
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain Mold fermentation object 12%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 12%, Wuyi bacterium conidia powder 10%, bacillus mycelia 12%, choline chloride 0.5%, light rare earth 1%, vitamins 1%, microelement 0.5%, wetting Agent 5%, dispersing agent 2%, stabilizer 2%, filler are supplied to 100%.
The vitamins is vitamin B；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein B, Mn, Mo, Cu, The mass ratio of Zn and Fe is 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following raw material: borax, manganese sulfate, molybdenum Sour sodium, copper sulphate, zinc sulfate, ferrous sulfate；Both wetting agent is the mixture of lauryl sodium sulfate and Tween-80, wherein Mass ratio be 1:2；Dispersing agent is the mixture of sodium lignin sulfonate and sodium lignin sulfonate calcium, wherein the mass ratio of the two is 1:1；Stabilizer is potassium dihydrogen phosphate；Filler is bentonite.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using preparation method as described in Example 1.
Embodiment 3
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain Mold fermentation object 15%, rose yellow streptomycete mycelia 12%, actinomyces styreptomyces globispotus strain original powder 15%, Wuyi bacterium conidia powder 12%, bacillus mycelia 15%, choline chloride 1%, light rare earth 2%, vitamins 2%, microelement 1%, wetting agent 4%, dispersing agent 4%, stabilizer 4%, filler are supplied to 100%.
Vitamins is the mixture of vitamin B and C, and the mass ratio of the two is 1:1；Microelement be B, Mn, Mo, Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe are 4:5:2:5:2:7, and B, Mn, Mo, Cu, Zn and Fe are from following Raw material: borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent is sodium methylene bis-naphthalene sulfonate；Point Powder is poly-aspartic-acid；Stabilizer is the mixture in vitamin E and potassium dihydrogen phosphate, wherein the mass ratio of the two is 1: 1；Filler is bentonite and kaolinic mixture, wherein the mass ratio of the two is 1:1.The complex microorganism of the present embodiment is wettable Property powder pesticide be prepared using preparation method as described in Example 1.
Embodiment 4
A kind of complex microorganism Wettable pesticide powder, is made of the component of following mass percent: golden yellow vertical chain Mold fermentation object 10%, rose yellow streptomycete mycelia 10%, actinomyces styreptomyces globispotus strain original powder 10%, Wuyi bacterium conidia powder 10%, bacillus mycelia 10%, choline chloride 0.5%, light rare earth 0.5%, vitamins 1%, microelement 1%, wetting Agent 3%, dispersing agent 3%, stabilizer 3%, filler are supplied to 100%.
The vitamins is the mixture of vitamin B and C, and the mass ratio of the two is 1:1:1；The microelement be B, Mn, Mo, Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe are 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe From following raw material: borax, manganese sulfate, sodium molybdate, copper sulphate, zinc sulfate, ferrous sulfate；Wetting agent be Tween-80 and Mixture in sodium methylene bis-naphthalene sulfonate, wherein the mass ratio of the two is 1:1；Dispersing agent is calcium lignosulfonate；Stabilizer For vitamin E；The filler is the mixture of kaolin and diatomite, wherein the mass ratio of the two is 1:2.
The complex microorganism Wettable pesticide powder of the present embodiment is prepared using preparation method as described in Example 1.
The release rate of the complex microorganism Wettable pesticide powder of the present invention of embodiment 5 detects
Complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention is placed in and is stored at room temperature, is claimed after 2d Sample is added in the sterile water with bead, and by gradient dilution, coating detects the various viable bacteria bodies discharged in pesticide sample Quantity is repeated 3 times, wherein by bacillus, rose yellow streptomycete, actinomyces styreptomyces globispotus strain release rate for, three Release rate calculation method be respectively as follows: release rate (%)=release bacillus number (cfu)/inoculation bacillus number (cfu) × 100%, release rate (%)=release rose yellow streptomycete number (cfu)/inoculation rose yellow streptomycete number (cfu) × 100%, release Rate (%)=release actinomyces styreptomyces globispotus strain number (cfu)/inoculation actinomyces styreptomyces globispotus strain number (cfu) × 100%, detection knot Fruit is as shown in the table:
The release rate of 1 bacillus of table
The release rate of 2 rose yellow streptomycete of table
The release rate of 3 actinomyces styreptomyces globispotus strain of table
As can be seen that the complex microorganism Wettable pesticide powder of 1-4 of embodiment of the present invention preparation from the data of table 1-3 Microbiological release rate it is high, and using being formulated the micro- of complex microorganism Wettable pesticide powder obtained described in embodiment 4 Biogenic emission rate highest, using the Microbiological release of the complex microorganism Wettable pesticide powder obtained of formula described in embodiment 3 Rate is taken second place.
The different fillers of embodiment 6, wetting agent, dispersing agent, stabilizer are to complex microorganism wettable powder agriculture of the invention The Index Influence of medicine
A kind of complex microorganism Wettable pesticide powder is prepared first, in accordance with method described in embodiment 1, wherein in addition to filling out The substance classes of material are different outer, remaining ingredient and content are all the same, then measure its suspensibility (the People's Republic of China country mark Quasi- GB/T 14825-2006), wetting time (the People's Republic of China's standard GB/T/T 5451-2001) and viable count (adopt Bacterium colony counting is carried out with serial dilutions method), as a result as shown in the table:
Index Influence of the different fillers of table 4 to complex microorganism Wettable pesticide powder of the invention
Known by table 4, filler be kaolin and diatomite mixture, and the mass ratio of the two be 1:2 when, gained pesticide Suspensibility is best, wetting time is most short, number of viable is most, and followed by filler is bentonite and kaolinic mixture, quality When than for 1:1, the suspensibility of gained pesticide is preferably, wetting time is shorter, number of viable is more.Next, we fix filler For the mixture of kaolin and diatomite, and the mass ratio of the two is 1:2, is tested the variety classes of wetting agent, as a result It is shown in Table 2:
Index Influence of the 5 different wetting agent of table to complex microorganism Wettable pesticide powder of the invention
As shown in Table 5, generally, complex microorganism Wettable pesticide powder of the present invention by lauryl sodium sulfate, spit Temperature -80, sodium methylene bis-naphthalene sulfonate are all good selection as wetting agent, but wetting agent is Tween-80 and di-2-ethylhexylphosphine oxide Mixture in sodium naphthalene sulfonate, and the mass ratio of the two be 1:1 when, wetability is best, followed by sodium methylene bis-naphthalene sulfonate, spits The wetability effect of temperature -80 is worst.Next, we fix the mixture that filler is kaolin and diatomite, and the matter of the two Amount ratio is 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1: 1, the variety classes of dispersing agent are tested, the results are shown in Table 6:
Index Influence of the different dispersing agents of table 6 to complex microorganism Wettable pesticide powder of the invention
Dispersing agent Dispersibility Viable count (× 109cfu/g)
Sodium lignin sulfonate 5.6
Calcium lignosulfonate ++ 5.9
Poly-aspartic-acid + 5.7
Note: dispersibility indicates that by force good dispersion is indicated with "+" with " ++ ", and dispersibility is generally not done
As can be seen from Table 6, the dispersibility of calcium lignosulfonate is best, followed by poly-aspartic-acid, lignin sulfonic acid Sodium is general as the effect of dispersibility.Next, we fix the mixture that filler is kaolin and diatomite, and the matter of the two Amount ratio is 1:2, fixed wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1: 1, fixed dispersing agent is calcium lignosulfonate, tests the variety classes of stabilizer, the results are shown in Table 7,
Index Influence of 7 different stabilizers of table to complex microorganism Wettable pesticide powder of the invention
As shown in Table 7, vitamin E is best as the effect of stabilizer, and number of viable is most, and potassium dihydrogen phosphate is as stabilization The effect of agent is not so good as vitamin E.It can be seen from the above result that mixture of the filler for kaolin and diatomite, and the quality of the two Than for 1:2, wetting agent is the mixture in Tween-80 and sodium methylene bis-naphthalene sulfonate, and the mass ratio of the two is 1:1, dispersion Agent is calcium lignosulfonate, and when stabilizer is vitamin E, obtained complex microorganism Wettable pesticide powder of the invention has Optimal effect.
The effect experiment of curing the disease of the complex microorganism Wettable pesticide powder of the invention of embodiment 7
According to the complex microorganism Wettable pesticide powder of formula preparation comparative example as described in example 4, with embodiment 4 Difference be only that the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contains golden yellow vertical strepto- Bacterium fermentation material, and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and bacillus Mycelia, the ingredient lacked use filler polishing.
Next, answering using complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example Conjunction Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of banana blight together, such as more according to conventional suspension liquid The application method and dosage of bacterium spirit, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 8 and comparative example Medicine, carbendazim are to the control efficiency comparative experiments of banana blight
Pesticide Scab inhibiting rate Integrated control effect
Embodiment 1 80% 81%
Embodiment 2 82% 82%
Embodiment 3 85% 84%
Embodiment 4 88% 87%
Comparative example 72% 74%
Carbendazim 70% 73%
Complex microorganism Wettable pesticide powder of the invention is with carbendazim, only containing golden yellow vertical as can be seen from the above table Streptomycete fermentation object and do not contain rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder, Wuyi bacterium conidia powder and gemma The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better For preventing and treating banana blight, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, and synthesis is anti- It controls effect and is up to 80%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
Next, according to the complex microorganism Wettable pesticide powder of formula preparation comparative example as described in example 4, with The difference of embodiment 4 is only that the microbiology class in the complex microorganism Wettable pesticide powder of comparative example only contains Wuyi Bacterium conidia powder, and without containing golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and Bacillus mycelia, the ingredient lacked use filler polishing.
Then, using the compound of complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of winter jujube rust together, according to conventional suspension liquid such as carbendazim Application method and dosage, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 9 and comparative example Medicine, carbendazim are to the control efficiency comparative experiments of winter jujube rust
Pesticide Scab inhibiting rate Integrated control effect
Embodiment 1 82% 82%
Embodiment 2 83% 84%
Embodiment 3 85% 86%
Embodiment 4 88% 89%
Comparative example 72% 74%
Carbendazim 68% 72%
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain Wuyi bacterium spore Sub- powder and do not contain golden yellow vertical streptomycete fermentation object, rose yellow streptomycete mycelia, actinomyces styreptomyces globispotus strain original powder and gemma The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better For preventing and treating winter jujube rust, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, integrated control Effect is up to 80%, and the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.Next, according to embodiment The complex microorganism Wettable pesticide powder of formula preparation comparative example described in 4, the difference with embodiment 4 is only that, is compared Microbiology class in the complex microorganism Wettable pesticide powder of example only contains rose yellow streptomycete mycelia, and without containing golden yellow Vertical streptomycete fermentation object, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and bacillus mycelia, the ingredient lacked are adopted With filler polishing.
Then, using the compound of complex microorganism Wettable pesticide powder described in 1-4 of the embodiment of the present invention and comparative example Microbial wettable powder pesticide, commercially available carbendazim are used for the prevention and treatment of cotton verticillium wilt together, according to for example more bacterium of conventional suspension liquid The application method and dosage of spirit, as a result see the table below:
The complex microorganism wettable powder agriculture of the complex microorganism Wettable pesticide powder of the invention of table 10 and comparative example Medicine, carbendazim are to the control efficiency comparative experiments of cotton verticillium wilt
Pesticide Scab inhibiting rate Integrated control effect
Embodiment 1 83% 82%
Embodiment 2 84% 82%
Embodiment 3 86% 85%
Embodiment 4 89% 88%
Comparative example 72% 74%
Carbendazim 68% 72%
As can be seen from the above table complex microorganism Wettable pesticide powder of the invention and carbendazim, only contain rose yellow chain Fungal hyphae and do not contain golden yellow vertical streptomycete fermentation object, Wuyi bacterium conidia powder, actinomyces styreptomyces globispotus strain original powder and gemma The Microbial wettable powder pesticide of bacillus mycelia is compared, and complex microorganism Wettable pesticide powder of the invention can be better For preventing and treating cotton verticillium wilt, and the complex microorganism Wettable pesticide powder control efficiency of embodiment 4 is best, and synthesis is anti- It controls effect and is up to 86%, the scab inhibiting rate and integrated control effect of embodiment 3 are only second to embodiment 4.
In addition to this, present inventor also found complex microorganism Wettable pesticide powder of the invention in addition to having very Except good preventing disease theraping effect, also have effects that promote crop yield.
It is as a result it has also been found that of the invention using complex microorganism Wettable pesticide powder prevention peanut dead leaf disease of the invention Complex microorganism Wettable pesticide powder can also be greatly facilitated peanut yield increasing, result other than it can prevent and treat peanut dead leaf disease It see the table below:
The complex microorganism Wettable pesticide powder of the invention of table 11 and carbendazim test the effect of increasing production of peanut
Complex microorganism Wettable pesticide powder of the invention is obvious to peanut yield increasing as can be seen from the above table, wherein implementing Complex microorganism Wettable pesticide powder described in example 4 is most obvious to peanut yield increasing effect, complex microorganism described in embodiment 3 Wettable pesticide powder takes second place to peanut yield increasing effect.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention And range.

Claims (9)

1.一种复合微生物可湿性粉剂农药，其特征在于，其由以下质量百分比的组分制成：金黄垂直链霉菌发酵物10％～15％、玫瑰黄链霉菌菌丝10％～15％、放线菌球孢链霉菌原粉10％～15％、武夷菌孢子粉10％～15％、芽孢杆菌菌丝10％～15％、氯化胆碱0.5％～2％、轻稀土0.5％～2％、维生素类0.5％～2％、微量元素0.5％～2％、润湿剂2％～5％、分散剂2％～5％、稳定剂2％～5％、填料补足到100％；1. a compound microorganism wettable powder pesticide is characterized in that, it is made up of the following components by mass percentage: Streptomyces aureus verticillum fermented product 10%～15%, Streptomyces roseus hyphae 10%～15%, Actinomycetes Streptomyces coccidioides original powder 10%～15%, Wuyi fungus spore powder 10%～15%, Bacillus mycelium 10%～15%, Choline chloride 0.5%～2%, Light rare earth 0.5%～ 2%, vitamins 0.5% to 2%, trace elements 0.5% to 2%, wetting agent 2% to 5%, dispersant 2% to 5%, stabilizer 2% to 5%, and fillers to 100%; 所述维生素类为维生素B和C的混合物，两者的质量比为1：1，所述润湿剂为吐温-80和亚甲基双萘磺酸钠中的混合物，其中两者的质量比为1:1；所述分散剂为木质素磺酸钙；所述稳定剂为维生素E；所述填料为高岭土和硅藻土的混合物，其中两者的质量比为1:2。The vitamins are a mixture of vitamins B and C, and the mass ratio of the two is 1:1, and the wetting agent is a mixture of Tween-80 and sodium methylene dinaphthalene sulfonate, wherein the mass of the two is The ratio is 1:1; the dispersant is calcium lignosulfonate; the stabilizer is vitamin E; the filler is a mixture of kaolin and diatomite, wherein the mass ratio of the two is 1:2. 2.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，所述金黄垂直链霉菌发酵物通过以下步骤制备：1)活化金黄垂直链霉菌：以划线法将金黄垂直链霉菌接种在高氏一号培养基平板上，28℃恒温培养4天，培养基的质量百分比组成：可溶性淀粉2.5％，KNO3 0.2％、K2HPO4 0.05％、MgSO4 0.05％、NaCl 0.05％、FeSO40.05％、琼脂2.5％，补水至100％；2)将活化的金黄垂直链霉菌接种于一级种子培养液中，180r·min-1，28℃培养72h，一级种子培养液的质量百分比组成：溶性淀粉2.5％、KNO3 0.2％，K2HPO4 0.05％、MgSO4 0.05％、NaCl 0.05％、FeSO4 0.05％，补水至100％；3)将制备好的一级种子培养液接种在二级种子培养液中，180r·min-1，28℃培养72h，离心分离，得到金黄垂直链霉菌发酵物，低温干燥，水分含量低于5％，二级种子培养液的质量百分比组成：葡萄糖0.05％、可溶性淀粉0.05％、K2HPO40.05％、NaCl 0.05％、MgSO4 0.05％、蛋白胨0.05％、酵母膏0.05％和黄豆粉0.05％，补水至100％；2. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, described Streptomyces aureus verticillum fermentation product is prepared by the following steps: 1) activate Streptomyces verticillum verticillata: by scribing method, vertical Streptomyces chinensis Streptomyces were inoculated on Gao's No. 1 medium plate and cultured at a constant temperature of 28°C for 4 days. The mass percentage composition of the medium was: soluble starch 2.5%, KNO 3 0.2%, K 2 HPO 4 0.05%, MgSO 4 0.05%, NaCl 0.05%, FeSO 4 0.05%, agar 2.5%, water to 100%; 2) Inoculate the activated Streptomyces aureus verticillum in the first-class seed culture solution, 180r·min -1 , 28 ℃ for 72h, first-class seed culture The mass percentage composition of the liquid: soluble starch 2.5%, KNO 3 0.2%, K 2 HPO 4 0.05%, MgSO 4 0.05%, NaCl 0.05%, FeSO 4 0.05%, replenishing water to 100%; 3) The prepared first-grade The seed culture solution was inoculated into the secondary seed culture solution, cultured at 180r·min -1 for 72 hours at 28°C, and centrifuged to obtain a fermented Streptomyces aureus verticalis, which was dried at low temperature and the moisture content was less than 5%. Mass percentage composition: glucose 0.05%, soluble starch 0.05%, K 2 HPO 4 0.05%, NaCl 0.05%, MgSO 4 0.05%, peptone 0.05%, yeast extract 0.05% and soybean powder 0.05%, replenish water to 100%; 所述玫瑰黄链霉菌菌丝通过以下步骤制备：1)将玫瑰黄链霉菌于PDA培养基上28℃培养5天；2)然后以2％的比例接种到种子培养基中，培养条件为：28℃、180r·min-1，培养3天，其中种子培养基为：3.5％花生饼粉，5％淀粉，1％葡萄糖，0.8％氯化钠，0.3％醇母膏，0.8％硫酸铵，0.04％磷酸二氢钾，0.4％碳酸钙，0.2％硫酸亚铁，0.2％硫酸锌，3.5％蔗糖，0.4％果糖；3)将步骤(2)制备得到的玫瑰黄链霉菌培养物通过过滤得到所述玫瑰黄链霉菌菌丝；The mycelium of Streptomyces flavus is prepared by the following steps: 1) culturing Streptomyces flavinus on PDA medium at 28° C. for 5 days; 2) then inoculating it into the seed medium at a ratio of 2%, and the culture conditions are: 28°C, 180r·min -1 , cultivated for 3 days, wherein the seed medium is: 3.5% peanut cake flour, 5% starch, 1% glucose, 0.8% sodium chloride, 0.3% alcohol mother paste, 0.8% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.4% calcium carbonate, 0.2% ferrous sulfate, 0.2% zinc sulfate, 3.5% sucrose, 0.4% fructose; 3) The Streptomyces roseus culture prepared in step (2) was filtered to obtain The mycelium of Streptomyces flavus; 所述放线菌球孢链霉菌原粉通过以下步骤制备：1)将已活化好的放线菌球孢链霉菌以2％的比例接种到接种于高氏一号液体培养基，28℃，150-160r·min-1振荡培养120h后获得放线菌种子培养液；2)将步骤(2)制备得到的放线菌球孢链霉菌通过过滤、干燥得到所述放线菌球孢链霉菌原粉；The original powder of the actinomycete Streptomyces coccidioides is prepared by the following steps: 1) inoculating the activated actinomycete Streptomyces coccidioides with a ratio of 2%, inoculated in Gao's No. 1 liquid medium, at 28° C., 150-160 r·min -1 shaking culture for 120 hours to obtain the actinomycete seed culture solution; 2) filtering and drying the actinomycete Streptomyces actinomycetes prepared in step (2) to obtain the actinomycete Streptomyces coccidioides original powder; 所述芽孢杆菌菌丝通过以下步骤制备：将已活化好的芽孢杆菌按2％的比例接种于培养基里，在28℃下培养5天，取出培养物进行过滤，得到的固体即为芽孢杆菌菌丝；其中所述芽孢杆菌为地衣芽孢杆菌、枯草芽孢杆菌或腊状芽孢杆菌。The Bacillus mycelium is prepared by the following steps: inoculating the activated Bacillus in the medium at a ratio of 2%, culturing at 28° C. for 5 days, taking out the culture and filtering, and the obtained solid is the Bacillus Mycelium; wherein the Bacillus is Bacillus licheniformis, Bacillus subtilis or Bacillus cereus. 3.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，其由以下质量百分比的组分制成：金黄垂直链霉菌发酵物10％～12％、玫瑰黄链霉菌菌丝10％～12％、放线菌球孢链霉菌原粉10％～12％、武夷菌孢子粉10％～12％、芽孢杆菌菌丝10％～12％、氯化胆碱0.5％～1％、轻稀土0.5％～1％、维生素类0.5％～1％、微量元素0.5％～1％、润湿剂2％～4％、分散剂2％～4％、稳定剂2％～4％、填料补足到100％。3. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, it is made up of the following components by mass percentage: 10%～12% of Streptomyces aureus verticillium fermentation product, Streptomyces rosacea bacterium Silk 10%～12%, Actinomycetes Streptomyces coccidioides original powder 10%～12%, Wuyi fungus spore powder 10%～12%, Bacillus mycelium 10%～12%, Choline chloride 0.5%～1 %, light rare earth 0.5%-1%, vitamins 0.5%-1%, trace elements 0.5%-1%, wetting agent 2%-4%, dispersant 2%-4%, stabilizer 2%-4% , Filling to 100%. 4.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，其由以下质量百分比的组分制成：金黄垂直链霉菌发酵物10％、玫瑰黄链霉菌菌丝10％、放线菌球孢链霉菌原粉10％、武夷菌孢子粉10％、芽孢杆菌菌丝10％、氯化胆碱0.5％、轻稀土0.5％、维生素类1％、微量元素1％、润湿剂3％、分散剂3％、稳定剂3％、填料补足到100％。4. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, it is made up of the following components by mass percentage: 10% of Streptomyces aureus verticillium fermentation product, 10% of Streptomyces roseus mycelium , Actinomycetes Streptomyces coccidioides original powder 10%, Wuyi bacteria spore powder 10%, Bacillus mycelium 10%, Choline chloride 0.5%, Light rare earth 0.5%, Vitamins 1%, Trace elements 1%, moisturizing 3% of wetting agent, 3% of dispersant, 3% of stabilizer, and filler to 100%. 5.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，所述维生素类为维生素B或C中的至少一种；所述微量元素为B、Mn、Mo、Cu、Zn和Fe，其中B、Mn、Mo、Cu、Zn和Fe的质量比为4：5：2：5：2：7，B、Mn、Mo、Cu、Zn和Fe来自于以下原材料：硼砂、硫酸锰、钼酸钠、硫酸铜、硫酸锌、硫酸亚铁；所述润湿剂为十二烷基硫酸钠、吐温-80、亚甲基双萘磺酸钠中的至少一种；所述分散剂为木质素磺酸钠、木质素磺酸钠钙、聚天门冬氨酸中的至少一种；所述稳定剂为维生素E、磷酸二氢钾中的至少一种；所述填料为硅藻土、膨润土、高岭土中的至少一种。5. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, described vitamin is at least one in vitamin B or C; Described trace element is B, Mn, Mo, Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe is 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from the following raw materials: borax, Manganese sulfate, sodium molybdate, copper sulfate, zinc sulfate, ferrous sulfate; the wetting agent is at least one of sodium lauryl sulfate, Tween-80, and sodium methylene dinaphthalene sulfonate; The dispersing agent is at least one of sodium lignosulfonate, sodium calcium lignosulfonate, and polyaspartic acid; the stabilizer is at least one of vitamin E and potassium dihydrogen phosphate; the filler is At least one of diatomite, bentonite, and kaolin. 6.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，其由以下质量百分比的组分制成：金黄垂直链霉菌发酵物15％、玫瑰黄链霉菌菌丝12％、放线菌球孢链霉菌原粉15％、武夷菌孢子粉12％、芽孢杆菌菌丝15％、氯化胆碱1％、轻稀土2％、维生素类2％、微量元素1％、润湿剂4％、分散剂4％、稳定剂4％、填料补足到100％；其中维生素类为维生素B和C的混合物，两者的质量比为1：1；微量元素为B、Mn、Mo、Cu、Zn和Fe，其中B、Mn、Mo、Cu、Zn和Fe的质量比为4：5：2：5：2：7，B、Mn、Mo、Cu、Zn和Fe来自于以下原材料：硼砂、硫酸锰、钼酸钠、硫酸铜、硫酸锌、硫酸亚铁；润湿剂为亚甲基双萘磺酸钠；分散剂为聚天门冬氨酸；稳定剂为维生素E和磷酸二氢钾中的混合物，其中两者的质量比为1:1；填料为膨润土和高岭土的混合物，其中两者的质量比为1:1。6. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, it is made up of the following components by mass percentage: 15% of Streptomyces verticillium fermented product, 12% of Streptomyces rosacea mycelium , Actinomycetes Streptomyces coccidioides original powder 15%, Wuyi bacteria spore powder 12%, Bacillus mycelium 15%, Choline chloride 1%, light rare earth 2%, vitamins 2%, trace elements 1%, moisturizing 4% of wetting agent, 4% of dispersant, 4% of stabilizer, and 100% of filler; the vitamins are the mixture of vitamin B and C, and the mass ratio of the two is 1:1; trace elements are B, Mn, Mo , Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe is 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe are from the following raw materials : Borax, manganese sulfate, sodium molybdate, copper sulfate, zinc sulfate, ferrous sulfate; wetting agent is sodium methylene bis-naphthalene sulfonate; dispersing agent is polyaspartic acid; stabilizer is vitamin E and diphosphate The mixture in potassium hydrogen, in which the mass ratio of the two is 1:1; the filler is the mixture of bentonite and kaolin, in which the mass ratio of the two is 1:1. 7.根据权利要求1所述的一种复合微生物可湿性粉剂农药，其特征在于，其由以下质量百分比的组分制成：金黄垂直链霉菌发酵物10％、玫瑰黄链霉菌菌丝10％、放线菌球孢链霉菌原粉10％、武夷菌孢子粉10％、芽孢杆菌菌丝10％、氯化胆碱0.5％、轻稀土0.5％、维生素类1％、微量元素1％、润湿剂3％、分散剂3％、稳定剂3％、填料补足到100％；其中所述维生素类为维生素B和C的混合物，两者的质量比为1：1：1；所述微量元素为B、Mn、Mo、Cu、Zn和Fe，其中B、Mn、Mo、Cu、Zn和Fe的质量比为4：5：2：5：2：7，B、Mn、Mo、Cu、Zn和Fe来自于以下原材料：硼砂、硫酸锰、钼酸钠、硫酸铜、硫酸锌、硫酸亚铁；润湿剂为吐温-80和亚甲基双萘磺酸钠中的混合物，其中两者的质量比为1:1；分散剂为木质素磺酸钙；稳定剂为维生素E；所述填料为高岭土和硅藻土的混合物，其中两者的质量比为1:2。7. a kind of compound microorganism wettable powder pesticide according to claim 1, is characterized in that, it is made up of the following components by mass percentage: 10% of Streptomyces aureus verticillium fermentation product, 10% of Streptomyces rosacea mycelium , Actinomycetes Streptomyces coccidioides original powder 10%, Wuyi bacteria spore powder 10%, Bacillus mycelium 10%, Choline chloride 0.5%, Light rare earth 0.5%, Vitamins 1%, Trace elements 1%, moisturizing 3% of wetting agent, 3% of dispersant, 3% of stabilizer, and 100% of filler; wherein the vitamins are a mixture of vitamins B and C, and the mass ratio of the two is 1:1:1; the trace elements It is B, Mn, Mo, Cu, Zn and Fe, wherein the mass ratio of B, Mn, Mo, Cu, Zn and Fe is 4:5:2:5:2:7, B, Mn, Mo, Cu, Zn and Fe from the following raw materials: borax, manganese sulfate, sodium molybdate, copper sulfate, zinc sulfate, ferrous sulfate; the wetting agent is a mixture of Tween-80 and sodium methylenebisnaphthalene sulfonate, two of which are The mass ratio of 1:1; the dispersant is calcium lignosulfonate; the stabilizer is vitamin E; the filler is a mixture of kaolin and diatomite, wherein the mass ratio of the two is 1:2. 8.一种如权利要求1～7任一项所述的复合微生物可湿性粉剂农药的制备方法，其特征在于，所述方法包括以下步骤：按照所述比例将润湿剂、分散剂、稳定剂、填料、氯化胆碱、轻稀土投入到气流粉碎机中粉碎，过350目筛，降温后，加入金黄垂直链霉菌发酵物、玫瑰黄链霉菌菌丝、放线菌球孢链霉菌原粉、武夷菌孢子粉、芽孢杆菌菌丝、维生素类、微量元素，继续粉碎，得到所述复合微生物可湿性粉剂农药。8. A method for preparing a composite microorganism wettable powder pesticide according to any one of claims 1 to 7, wherein the method comprises the steps of: adding a wetting agent, a dispersing agent, a stabilizing agent and a The agent, filler, choline chloride and light rare earth are put into the jet mill to be pulverized, passed through a 350-mesh sieve, and after cooling, the fermented product of Streptomyces aureus verticillum, Streptomyces roseus hyphae, and Streptomyces coccidioides progenitor are added. The powder, Wuyi fungus spore powder, Bacillus mycelium, vitamins and trace elements are continuously pulverized to obtain the compound microorganism wettable powder pesticide. 9.权利要求1～7任一项所述的复合微生物可湿性粉剂农药在防治香蕉枯萎病、冬枣锈病、棉花黄萎病以及花生增产中的用途。9. Use of the composite microbial wettable powder pesticide according to any one of claims 1 to 7 in the prevention and treatment of banana fusarium wilt, winter jujube rust, cotton verticillium wilt and increased peanut yield.
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CN102381912A (en) * 2011-11-09 2012-03-21 潍坊万胜生物农药有限公司 Microbial pesticide preparation for plant protection and growth regulation
CN103772037A (en) * 2014-01-05 2014-05-07 石达璘 Continuous cropping resistant high-yield agent for fruits and vegetables
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CN102206593A (en) * 2011-03-15 2011-10-05 海南大学 Streptomyces aureoverticillatus strain and its application
CN102381912A (en) * 2011-11-09 2012-03-21 潍坊万胜生物农药有限公司 Microbial pesticide preparation for plant protection and growth regulation
CN104686582A (en) * 2013-12-06 2015-06-10 大连三科生物工程有限公司 Preparation method of microbial agent for antagonizing potato scab
CN103772037A (en) * 2014-01-05 2014-05-07 石达璘 Continuous cropping resistant high-yield agent for fruits and vegetables

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