CN106771163A - A kind of liquid detecting box and its detection method with control device - Google Patents
A kind of liquid detecting box and its detection method with control device Download PDFInfo
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- CN106771163A CN106771163A CN201611154467.7A CN201611154467A CN106771163A CN 106771163 A CN106771163 A CN 106771163A CN 201611154467 A CN201611154467 A CN 201611154467A CN 106771163 A CN106771163 A CN 106771163A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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Abstract
The invention discloses a kind of liquid detecting box with control device, including:Box body (1), defines three sample cavity (11), liquid control device cavity (12) and the piston air cavities (13) for accommodating sample liquid, and a detection bed for being used to carry out pattern detection in it;Sample sleeve pipe (2), it is located in sample cavity (11) for providing detection sample;Injection-tube (6), it is located in liquid control device cavity (12), for drawing or discharging the detection sample in sample sleeve pipe (2);With rotary switch (5), it is arranged at box body (1) bottom, for connecting sample sleeve pipe (2) with injection-tube (6), injection-tube (6) and detection bed.Detection process is carried out using liquid detecting box of the invention to be overflowed without any liquid, and its testing result is completely the same with conventional method, and operating personnel are without the serum tube, safe ready of uncapping by hand.
Description
Technical field
The invention belongs to technical field of liquid detection, more particularly to a kind of liquid detecting box and its inspection with control device
Survey method.
Background technology
China IVD is developed rapidly, and quick detection (POCT) is all based on immunochromatography technique, i.e. test strips;By manually adding
Increase serum or whole blood complete detection, so cause serious Aerosol Pollution and institute's sense risk.Especially detection has infectiousness
Pathogen, risk is very high.The implementation of diagnosis and treatment is classified with China, in the case of community sanitary institute prevention and control measure is incomplete,
Risk is not controlled.Secondly, from the invention, the prevailing scenario of its application is clinical bedside diagnosis to POCT, but due to
Need to open heparin tube, artificial sample-adding is incubated ten minutes and then detects;Cause POCT to can be only placed at clinical laboratory to be entered by professional
Row operation, reduces institute's sense risk.
Therefore, there are many defects for current immunochromatography, the present invention provides a kind of liquid inspection with control device
Box and detection method are surveyed, makes every effort to solve the problem of current POCT clinical practices.
The content of the invention
The present invention proposes a kind of simple to operate, safe ready and detection is accurate to solve above mentioned problem of the prior art
Degree high liquid detecting box and its detection method with control device.
To achieve the above object, the present invention uses following technical scheme:
The first aspect of the invention is to provide a kind of liquid detecting box with control device, including:
Box body 1, defines three sample cavity 11, liquid control device cavity 12 and the pistons for accommodating sample liquid in it
Air cavity 13, and a detection bed for being used to carry out pattern detection;
Sample sleeve pipe 2, it is located in the sample cavity 11 for providing detection sample;
Injection-tube 6, it is located in the liquid control device cavity 12, for drawing or discharging in the sample sleeve pipe 2
Detection sample;With
Rotary switch 5, it is arranged at the bottom of the box body 1, by itself rotating for connecting the sample for rotary switch 5
This sleeve pipe 2 and injection-tube 6, injection-tube 6 and detection bed.
Further, also including buckle be arranged on the box body 1 be used for close it is described detection bed upper lid 3, it is described on
Lid 3 is provided with corresponding to the detection window 31 for detecting bed and corresponds respectively to the sample cavity 11, liquid control device chamber
The perforate 32,33,34 of body 12 and piston air cavity 13.
Further, the base 4 of the bottom of the box body 1 is also arranged at including buckle, the base 4 is provided with hole 41 and sets
In the annular protrusion 42 at the edge of the hole 41, the rotary switch 5 is fastened in the hole 41 and by annular protrusion 42
The bottom of the box body 1.
Further, the detection bed sets the top with the box body 1, and it is by liquid bath 14 and some rows and the liquid bath
The test paper mounting groove 15 of 14 connections is constituted, and the bottom of the liquid bath 14 is provided with the fluid apertures f and stomata e higher than the fluid apertures f planes.
Further, the bottom of sample cavity 11 is provided with the first lancet 16, and first lancet 16 is provided with logical
Stomata a and walk fluid apertures b;The bottom of liquid control device cavity 12 is provided with the second punctured hole 17, is set on second punctured hole 17
There is hydraulic control hole c;The bottom of piston air cavity 13 is provided with piston hole d.
Further, the bottom of the box body 1 be additionally provided with hole a1, b1 that through hole 18 and two groups set around the through hole 18,
C1, d1 and e1, f1, c2, d2;The bottom of the box body 1 is additionally provided with away liquid bath respectively by hole a-a1, b-b1, c1-c-c2, d1-d-
D2, e-e1, f-f1 are connected.
Further, the rotary switch 5 is made up of platform 51, guiding gutter 52, boss 53 and rotating shaft 54, the guiding gutter
52 for two and side by side located at the marginal position of the platform 51, with the through hole in the movable insertion through hole 18 of the rotating shaft 54
18 are equipped with.
Further, the injection-tube 6, sample sleeve pipe 2 and piston air cavity 13 pass through hole c, c1, b1, b, a, a1, d1, d
With the sampling path that the segmentation rotation rotary switch 5 forms liquid sample;The injection-tube 6, detection bed and piston gas
Cavity 13 forms a detection of liquid sample by hole c, c2, f1, f, e, e1, d2, d and the segmentation rotation rotary switch 5
Path.
The second aspect of the invention is to provide a kind of detection method of the liquid detecting box with control device, bag
Include following steps:
(1) loading:Patients serum, positive reference product or negative reference product are taken, is respectively placed in sleeve pipe, cover tightly sleeve pipe, will
Sleeve pipe is placed in sample well;
(2) by switch rotary to sample position, i.e. sample aperture a1 and b1 is connected by the guiding gutter on switch with c1 and d1,
C1 connects hydraulic control unit, rotates hydraulic control unit and draws sample;
(3) by switch rotary to detecting bed position, and detection bed hole e1 and f1 is by the water conservancy diversion and hydraulic control unit on switch
On c2 and d2 holes connection, reversely rotate hydraulic control unit, inject liquid into test strips on reaction bed;
(4) it is incubated 5~10 minutes, the watch window on observation detection bed.
Further, before step (1) loading, box body bottom coat layer of silica gel, and exposed hole a1, b1, c1, c2,
D1, d2, e1 and f1, then load onto rotary switch, cover bottom, and through inventor's repetition test, rotary switch leads to box body bottom
The effect for crossing silica gel sealing is very good, is overflowed without any liquid in testing repeatedly.
The present invention uses above-mentioned technical proposal, compared with prior art, have the following technical effect that:
Liquid detecting box with control device of the invention ensure that detection sample, the residual sample of reaction end and phase
The liquid of pass will not be revealed, therefore it is not true with other using the Aerosol Pollution that like product process is produced to avoid clinical structure
Fixed risk;Acted on by the sample sleeve pipe for introducing, effectively prevent during clinical unit's use because operational error is led
The problem for causing patient body fluid or blood to spill;It is more important to be detected entirely using the liquid detecting box with control device
Cheng Zhong, gas, not with atmosphere, further avoids clinical unit from using process aerosol that may be present in box body interior circulation
Risk;In addition, in the design of switch, when sample completes to detect, switch is placed on completing detection shape;Examined using the liquid
The testing result for surveying box is completely the same with conventional method, and detection process is overflowed without any liquid, fool proof, and operating personnel need not
Uncap serum tube, safe ready by hand.
Brief description of the drawings
Fig. 1 is a kind of dimensional structure diagram of the liquid detecting box with control device of the present invention;
Fig. 2 is a kind of assembling structure schematic diagram of the liquid detecting box with control device of the present invention;
Fig. 3 is the structural representation of box body in a kind of liquid detecting box with control device of the present invention, wherein, 3a is box
The upward view of body, 3b is the front view of box body;3c is the top view of box body;
Fig. 4 is the structural representation of sample sleeve pipe in a kind of liquid detecting box with control device of the present invention;
Fig. 5 is the structural representation of upper lid in a kind of liquid detecting box with control device of the present invention;
Fig. 6 is the structural representation of base in a kind of liquid detecting box with control device of the present invention;
Fig. 7 is the structural representation of rotary switch in a kind of liquid detecting box with control device of the present invention, wherein, 7a
It is the upward view of rotary switch, 7b is the side view of rotary switch, and 7c is the stereogram of rotary switch;
Fig. 8 is the structural representation of injection-tube in a kind of liquid detecting box with control device of the present invention;
Wherein, box body 1, sample cavity 11, liquid control device cavity 12, piston air cavity 13, liquid bath 14, test paper mounting groove
15, sample sleeve pipe 2, lid 21, body 22, Pull ring cover 23, upper lid 3 detects window 31, perforate 32, perforate 33, perforate 34, base
4, hole 41, annular protrusion 42, rotary switch 5, platform 51, guiding gutter 52, boss 53, rotating shaft 54, injection-tube 6;Passage a, walks
Fluid apertures b, hydraulic control hole c, piston hole d, stomata e, fluid apertures f, hole a1, hole b1, hole c1, hole d1, hole e1, hole f1, hole c2, hole d2.
Specific embodiment
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
As shown in Figure 1-2, the first aspect of the invention is to provide a kind of liquid detecting box with control device, bag
Include:
Box body 1, defines three sample cavity 11, liquid control device cavity 12 and the pistons for accommodating sample liquid in it
Air cavity 13, and a detection bed for being used to carry out pattern detection, wherein, the bottom of sample cavity 11 with diplopore first is worn
Pricker 17, its effect is mainly used in puncture blood collecting pipe, and to install injection-tube 6, periphery is extracted in its effect to liquid control device cavity 12
Blood, and detection bed is transported to, detection bed is used for placing detection test strips, sample cavity 11, liquid control device cavity 12
Connected by the guiding gutter 52 on box body bottom rotary switch 5 and piston air cavity 13 and detection bed between;
Sample sleeve pipe 2, it is located in sample cavity 11 for providing detection sample, its parcel conventional blood collection pipe, it is ensured that
During use, sample sleeve pipe does not fall off, and prevents blood samples of patients from flowing out, and causes unnecessary risk;
Injection-tube 6, as shown in figure 8, it is located in liquid control device cavity 12, for drawing or discharging in sample sleeve pipe 2
Detection sample, the top of injection-tube 6 can be lifted the piston in injection-tube 6 with rotatable cross mouthful by rotation
To draw and exclude liquid;With
Rotary switch 5, is critical component of the invention, and it is arranged at the bottom of box body 1, by itself revolving for rotary switch 5
Connection sample sleeve pipe 2 is diverted to injection-tube 6, injection-tube 6 and detection bed.
On the basis of above-mentioned technical proposal, as shown in Figure 2 and Figure 5, being also arranged on box body 1 including buckle is used to close
The upper lid 3 of bed is detected, upper lid 3 is provided with corresponding to the detection window 31 for detecting bed and corresponds respectively to sample cavity 11, hydraulic control
The perforate 32,33,34 of device cavity 12 processed and piston air cavity 13.
On the basis of above-mentioned technical proposal, as shown in fig. 6, being also arranged at the base 4 of the bottom of box body 1, bottom including buckle
Seat 4 is provided with hole 41 and the annular protrusion 42 located at the edge of hole 41, and rotary switch 5 is in hole 41 and tight by annular protrusion 42
Gu in the bottom of box body 1.
On the basis of above-mentioned technical proposal, as shown in 3c in Fig. 3, detection bed sets the top with box body 1, and it is by liquid bath
14 and the test paper mounting groove 15 that is connected with liquid bath 14 of some rows constitute, the bottom of liquid bath 14 is provided with fluid apertures f and higher than fluid apertures f planes
Stomata e.
On the basis of above-mentioned technical proposal, as shown in 3a in Fig. 3 and 3b, the bottom of sample cavity 11 is provided with the first lancet
16, its effect is mainly used in puncture blood collecting pipe, and the first lancet 16 is provided with passage a and walks fluid apertures b;Liquid control device cavity
It is stepper piston that 12 bottoms are provided with the second punctured hole 17, hydraulic control unit cavity 12 and install injection-tube 6, and periphery is extracted in its effect
Blood, and detection bed is transported to, the second punctured hole 17 is provided with hydraulic control hole c;The bottom of piston air cavity 13 is provided with piston hole d.
On the basis of above-mentioned technical proposal, as shown in 3a in Fig. 3, the bottom of box body 1 be additionally provided with through hole 18 and two groups around
Hole a1, b1, c1, d1 and e1, f1, c2, d2 that through hole 18 is set;The bottom of box body 1 is additionally provided with away liquid bath respectively by hole a-a1, b-
B1, c1-c-c2, d1-d-d2, e-e1, f-f1 are connected.
On the basis of above-mentioned technical proposal, as shown in fig. 7, rotary switch 5 is by platform 51, guiding gutter 52, the and of boss 53
Rotating shaft 54 is constituted, and guiding gutter 52 is two and is located on platform 51 side by side, is preferably set near marginal position;The activity of rotating shaft 54
It is equipped with through hole 18 in embedded through hole 18.Preferably, rotary switch 5 is one-way movement, it is impossible to rotated back and forth.Wherein 7a is
The upward view of rotary switch, 7b is the side view of rotary switch, and 7c is the stereogram of rotary switch.
On the basis of above-mentioned technical proposal, as shown in figure 4, sample sleeve pipe 2 includes lid 21 and body 22, the end of body 22
End is additionally provided with Pull ring cover 23;Lid 21 is located at the lower end of sample sleeve pipe 2, and its outer surface is provided with several along its body 22 side
To card article, the draw-in groove in card article and sample cavity 11 is equipped with and be fixed on sample sleeve pipe 2 is spacing in sample cavity 11, draw
Ring cowling 23 is used to seal sample sleeve pipe 2 located at the upper end porch of sample sleeve pipe 2.Injection-tube 6, sample sleeve pipe 2 and piston air cavity
The 13 sampling paths that liquid sample is formed by hole c, c1, b1, b, a, a1, d1, d and the segmentation rotation rotary switch 5;
Injection-tube 6, detection bed and piston air cavity 13 are by hole c, c2, f1, f, e, e1, d2, d and the segmentation rotation rotary switch 5
Form a detection path of liquid sample.
There is the operation principle of the liquid detecting box of control device to be for this:The individual accessory of liquid detecting box is assembled first
Get well, and test strip is placed in detection window;Due to needing to lead between sample sleeve pipe 2 and injection-tube 6, detection bed and injection-tube 6
Rotary switch 5 is crossed to connect, thus it is completely closed between each hole.It is closed by silicagel pad between rotary switch 5 and box body 1;Now
Rotary switch 5 is in off states, is all not connected to any one hole.Secondly, heparin tube or other supporting liquid lines are placed in sample
Among this sleeve pipe, testing staff is placed among sample cavity, and rotary injection pipe 6 makes sample cavity 11 and liquid control device
Cavity 12 is connected, and injection-tube 6 draws the sample to be detected in sample sleeve pipe 2;Then, rotary switch 5 is rotated to bed is detected, instead
Sample to be detected is pushed into test strips on detection bed to rotary injection pipe 6;After reaction is incubated a period of time, can be checked with detection window
Testing result;Finally, rotary switch 5 is placed in use state, completes detection.
Application Example:
Present embodiments provide a kind of detection method of the liquid detecting box with control device, including step:
(1) box assembling:
A, by the test strip of HIV, HBV, HCV and TP be placed in detection bed on;
It is b, hydraulic control unit and stomata piston is packaged;Carefully cover lid;
Silica gel is sealed up in c, box body bottom, exposes a1, b1, c1, c2, d1, d2, e1 and f1 hole.Load onto switch and cover bottom, will
Switch is placed in off states, prepares loading;
(2) loading:Patients serum, positive reference product and negative reference product are taken, three samples of liquid detecting box are respectively placed in
In this sleeve pipe, sample sleeve pipe is covered tightly by Pull ring cover, sample sleeve pipe is placed in sample cavity;
(3) rotary switch is rotated to sample cavity position, i.e. hole a1 and b1 by the guiding gutter and d1 on rotary switch
Connected with c1;C1 connects hydraulic control unit, rotates hydraulic control unit and draws sample;
(4) by switch rotary to detecting bed position, and detection bed hole e1 and f1 is by the water conservancy diversion and hydraulic control unit on switch
On d2 and c2 holes connection;Hydraulic control unit is reversely rotated, test strips on reaction bed are injected liquid into;
(5) it is incubated 5~10 minutes, the watch window on observation detection bed.
Interpretation of result:60 samples are continuously detected using liquid detecting box of the invention, testing result is complete with conventional method
Complete consistent, detection process is overflowed without any liquid, fool proof, and operating personnel are without serum tube of uncapping by hand.
Specific embodiment of the invention has been described in detail above, but it is intended only as example, and the present invention is not limited
It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications carried out to the present invention and
Replacement is also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of liquid detecting box with control device, it is characterised in that including:
Box body (1), three are defined in it is used to accommodate the sample cavity (11) of sample liquid, liquid control device cavity (12) and lives
Plug air cavity (13), and a detection bed for being used to carry out pattern detection;
Sample sleeve pipe (2), it is located in the sample cavity (11) for providing detection sample;
Injection-tube (6), it is located in the liquid control device cavity (12), for drawing or discharging in the sample sleeve pipe (2)
Detection sample;With
Rotary switch (5), it is arranged at the box body (1) bottom, for connecting the sample sleeve pipe (2) with injection-tube (6), note
Pipe (6) is penetrated with detection bed.
2. the liquid detecting box with control device according to claim 1, it is characterised in that be also arranged at including buckle
It is used to close the upper lid (3) of the detection bed on the box body (1), the upper lid (3) is provided with corresponding to the detection bed
Detect window (31) and correspond respectively to the sample cavity (11), liquid control device cavity (12) and piston air cavity (13)
Perforate (32,33,34).
3. the liquid detecting box with control device according to claim 1, it is characterised in that be also arranged at including buckle
The base (4) of box body (1) bottom, the base (4) is provided with hole (41) and the convex annular located at the hole (41) edge
Rise (42), the rotary switch (5) is fastened on the box body (1) bottom in the hole (41) and by annular protrusion (42)
Portion.
4. the liquid detecting box with control device according to claim 1, it is characterised in that the detection bed set with
The top of the box body (1), it is made up of the test paper mounting groove (15) that liquid bath (14) and some rows connect with the liquid bath (14),
Liquid bath (14) bottom is provided with fluid apertures (f) and the stomata (e) higher than fluid apertures (f) plane.
5. the liquid detecting box with control device according to claim 4, it is characterised in that the sample cavity (11)
Bottom is provided with the first lancet (16), and first lancet (16) is provided with passage (a) and walks fluid apertures (b);The hydraulic control
Device cavity (12) bottom processed is provided with the second punctured hole (17), and second punctured hole (17) is provided with hydraulic control hole (c);The work
Plug air cavity (13) bottom is provided with piston hole (d).
6. the liquid detecting box with control device according to claim 5, it is characterised in that box body (1) bottom
Be additionally provided with hole (a1), (b1), (c1), (d1) and (e1), (f1) that through hole (18) and two groups set around the through hole (18),
(c2)、(d2);Box body (1) bottom be additionally provided with away liquid bath respectively by hole (a)-(a1), (b)-(b1), (c1)-(c)-(c2),
(d1)-(d)-(d2), (e)-(e1), (f)-(f1) connections.
7. the liquid detecting box with control device according to claim 6, it is characterised in that the rotary switch (5)
It is made up of platform (51), guiding gutter (52), boss (53) and rotating shaft (54), the guiding gutter (52) is for two and side by side located at institute
State on platform (51), be equipped with the through hole (18) in rotating shaft (54) the activity insertion through hole (18).
8. the liquid detecting box with control device according to claim 7, it is characterised in that the injection-tube (6), sample
This sleeve pipe (2) and piston air cavity (13) are rotated by hole (c), (c1), (b1), (b), (a), (a1), (d1), (d) and segmentation
The rotary switch (5) forms a sampling path of liquid sample;The injection-tube (6), detection bed and piston air cavity
(13) liquid is formed by hole (c), (c2), (f1), (f), (e), (e1), (d2), (d) and the segmentation rotation rotary switch (5)
One detection path of body sample.
9. a kind of detection method of the liquid detecting box with control device as described in claim any one of 1-8, its feature exists
In comprising the following steps:
(1) loading:Patients serum, positive reference product or negative reference product are taken, is respectively placed in sleeve pipe, cover tightly sleeve pipe, by sleeve pipe
It is placed in sample well;
(2) by switch rotary to sample position, i.e. sample aperture a1 and b1 is connected by the guiding gutter on switch with d1 and c1, and c1 connects
Logical hydraulic control unit, rotates hydraulic control unit and draws sample;
(3) by switch rotary to detecting bed position, and detection bed hole e1 and f1 is by the water conservancy diversion on switch and hydraulic control unit
D2 and c2 holes connect, and reversely rotate hydraulic control unit, inject liquid into test strips on reaction bed;
(4) it is incubated 5~10 minutes, the watch window on observation detection bed.
10. detection method according to claim 9, it is characterised in that before step (1) loading, applies in box body bottom
Layer of silica gel, and exposed hole a1, b1, c1, c2, d1, d2, e1 and f1 are covered, rotary switch is then loaded onto, is covered bottom.
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CN108226550A (en) * | 2018-01-22 | 2018-06-29 | 上海默礼生物医药科技有限公司 | A kind of chemical luminescence reagent kit and its detection method of miniature more bin controls |
CN108226550B (en) * | 2018-01-22 | 2023-12-01 | 上海默礼生物医药科技有限公司 | Miniature multi-bin controlled chemiluminescent kit and detection method thereof |
CN108485912A (en) * | 2018-03-27 | 2018-09-04 | 上海默礼生物医药科技有限公司 | A kind of miniature more warehouses control nucleic acid detection apparatus |
CN108485912B (en) * | 2018-03-27 | 2021-05-07 | 上海默礼生物医药科技有限公司 | Miniature many storehouses control nucleic acid detection device |
CN114487390A (en) * | 2022-01-29 | 2022-05-13 | 美昕医疗器械(昆山)有限公司 | Detection box and detection system for detecting liquid flow in medical diversion device |
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