CN106749151B - Method for rapidly extracting blueberry pomace anthocyanin - Google Patents

Abstract

The invention relates to a method for rapidly extracting anthocyanin from blueberry pomace. The invention uses frozen blueberry pomace as a raw material, is beaten into pulp by a beater, and is treated with an ultra-high pressure device at a certain temperature, and then added to the pomace. Pectinase, hemicellulase, and cellulase enzymatic hydrolysis, mixed with ethanol-acetic acid-water solvent after enzymatic hydrolysis, and processed with a high-pressure homogenizer to extract anthocyanin, and the pomace and anthocyanin were extracted by a centrifuge The liquid is separated and concentrated under reduced pressure to remove ethanol and acetic acid in the extract, and then put into a vacuum freeze dryer for freeze-drying to obtain blueberry pomace anthocyanins. The technical solution provided by the invention has simple process operation, short cycle, low cost and less pollution, is suitable for large-scale continuous production, realizes comprehensive utilization of blueberries, and has good application prospects.

Description

A kind of rapid extraction method of blueberry pomace anthocyanin

technical field

The invention relates to a rapid extraction method of anthocyanin from blueberry pomace.

Background technique

As a small berry with good flavor and quality and high nutritional value, blueberry has high nutritional value and economic value. Anthocyanins are all-natural and potent antioxidants that protect vision, treat diabetes and cardiovascular disease. The content of anthocyanins in blueberries is high, and most of them exist in the pomace, and they have good physiological functions, so they are a good source of anthocyanins. The traditional extraction method uses solvents such as water and alcohol, and extracts anthocyanins through processes such as heating and ultrasonic assistance. Since heat treatment has a great influence on the stability of anthocyanins, traditional extraction methods will cause great loss of anthocyanins during the extraction process. In addition, the traditional process requires relatively long extraction time and large energy consumption, which is not conducive to continuous production.

SUMMARY OF THE INVENTION

In order to overcome the technical problems of long period and large anthocyanin loss in the extraction process of blueberry pomace anthocyanin, the purpose of the present invention is to provide a rapid extraction method of blueberry pomace anthocyanin, which adopts ultra-high pressure treatment and high pressure homogenization. The method of extracting anthocyanins in a quality way, extracting blueberry anthocyanins by non-thermal processing, shortens the extraction time, realizes continuous production of blueberry anthocyanins, and has low cost, no pollution, and the produced blueberry anthocyanins have less impurities. , high purity.

The rapid extraction method of blueberry pomace anthocyanins provided by the invention is as follows:

Operation procedure:

Frozen blueberry pomace → thawing → beating → ultra-high pressure treatment → ethanol-acetic acid-water solution extraction → high pressure homogenization treatment → centrifugal separation filtration → anthocyanin extract → reduced pressure concentration → vacuum freeze drying → product.

The specific operation process is as follows:

(1) Frozen blueberry pomace, after thawing, use a homogenizer to homogenize, and use an ultra-high pressure device for ultra-high pressure treatment;

(2) adding the following ingredients by weight to the pomace obtained after the treatment in step (1): 5‰ pectinase, 3‰ hemicellulase, 3‰ cellulase, and enzymatically hydrolyzed at 50° C. for 2 hours;

(3) After enzymatic hydrolysis, add a mixed solution of ethanol-acetic acid-water, the volume ratio of ethanol-acetic acid-water is 40:10:50, and the material ratio is 1 g:5 mL. After mixing evenly, use a high-pressure homogenizer to process ;

(4) the turbid liquid obtained in step (3) is separated and filtered by a centrifuge and the anthocyanin extract, and the anthocyanin is extracted by a vacuum rotary evaporator to remove ethanol and acetic acid;

(5) The anthocyanin aqueous solution obtained in step (4) is freeze-dried in a vacuum freeze dryer to obtain blueberry anthocyanin powder.

The beneficial effects obtained by the present invention mainly include:

1. Using ultra-high pressure treatment, under the condition of non-thermal processing, the tissue structure of blueberry pomace is greatly destroyed, and at the same time, an acidic ethanol solution is used to mix with blueberry pomace, which is the rapidity of subsequent anthocyanins. Extraction creates extremely favorable conditions.

2. Using the high-pressure homogeneous extraction method, combined with the organic solvent extraction solution, the anthocyanin in the blueberry pomace can be fully dissolved in the organic solvent in a short time without heating, and the extraction time is short and greatly shortened. The production cycle is shortened, the energy consumption is reduced, the extraction temperature is low, and the obtained blueberry anthocyanin powder has high extraction quantity, high purity and less impurities.

Detailed ways

Example 1

Take 1kg of frozen blueberry pomace, thaw it at room temperature, use a homogenizer to make it into a homogenate, and place it in an ultra-high pressure device at 350MPa and 40°C for 5min. 5 g of pectinase, 3 g of hemicellulase, and 3 g of cellulase were added to the pomace treated with ultra-high pressure, and enzymatically hydrolyzed at 50 °C for 2 h. Add 5 L of ethanol-acetic acid-water (40:10:50, v/v/v) solution to the enzymatically hydrolyzed pomace, and use a high-pressure homogenizer to process at 2000 rpm for 7 min. The turbid liquid obtained after high pressure homogenization is separated from the pomace and anthocyanin extract by a centrifuge, and the anthocyanin extract is passed through a vacuum rotary evaporation device to remove ethanol and acetic acid at 45 ° C to obtain blueberry pomace. Aqueous solution of anthocyanin. The anthocyanin aqueous solution was lyophilized in a vacuum freeze dryer to obtain 4.8 g of blueberry pomace anthocyanin powder.

Example 2

Take 10kg of frozen blueberry pomace, thaw it at room temperature, use a homogenizer to make it into a homogenate, and place it in an ultra-high pressure device at 350MPa and 40°C for 7min. 50 g of pectinase, 30 g of hemicellulase, and 30 g of cellulase were added to the pomace treated with ultra-high pressure, and enzymatically hydrolyzed at 50° C. for 2 h. Add 50 L of ethanol-acetic acid-water (40:10:50, v/v/v) solution to the enzymatically hydrolyzed pomace, and use a high-pressure homogenizer to process at 2000 rpm for 7 min. The turbid liquid obtained after high pressure homogenization is separated from the pomace and anthocyanin extract by a centrifuge, and the anthocyanin extract is passed through a vacuum rotary evaporation device to remove ethanol and acetic acid at 45 ° C to obtain blueberry pomace. Aqueous solution of anthocyanin. The anthocyanin aqueous solution was lyophilized in a vacuum freeze dryer to obtain 49.5 g of blueberry pomace anthocyanin powder.

Example 3

Take 20kg of frozen blueberry pomace, thaw it at room temperature, use a homogenizer to make it into a homogenate, and place it in an ultra-high pressure device at 350MPa and 40°C for 7min. 100 g of pectinase, 60 g of hemicellulase, and 60 g of cellulase were added to the pomace treated with ultra-high pressure, and enzymatically hydrolyzed at 50° C. for 2 h. Add 100 L of ethanol-acetic acid-water (40:10:50, v/v/v) solution to the enzymatically hydrolyzed pomace, and use a high-pressure homogenizer to process at 2000 rpm for 7 min. The turbid liquid obtained after high pressure homogenization is separated from the pomace and anthocyanin extract by a centrifuge, and the anthocyanin extract is passed through a vacuum rotary evaporation device to remove ethanol and acetic acid at 45 ° C to obtain blueberry pomace. Aqueous solution of anthocyanin. The anthocyanin aqueous solution was lyophilized in a vacuum freeze dryer to obtain 99.3 g of blueberry pomace anthocyanin powder.

Claims (3)

1. a rapid extraction method of blueberry pomace anthocyanin, is characterized in that, specifically comprises the following steps: (1) Frozen blueberry pomace, after thawing, use a homogenizer to make a homogenate, and use an ultra-high pressure device to carry out ultra-high pressure treatment, the ultra-high pressure treatment is: pressure 350MPa, temperature is 40 ℃, and the treatment time is 5min; (2) adding the following ingredients by weight to the pomace obtained after the treatment in step (1): 5‰ pectinase, 3‰ hemicellulase, 3‰ cellulase, and enzymatically hydrolyzed at 50° C. for 2 hours; (3) After enzymatic hydrolysis, add a mixed solution of ethanol-acetic acid-water, the volume ratio of ethanol-acetic acid-water is 40:10:50, and the material ratio is 1 g:5 mL. Extraction, the number of revolutions is 2000 revolutions/min, and the extraction time is 7-10min; (4) the turbid liquid obtained in step (3) is separated and filtered by a centrifuge and the anthocyanin extract, and the anthocyanin is extracted by a vacuum rotary evaporator to remove ethanol and acetic acid; (5) The anthocyanin aqueous solution obtained in step (4) is freeze-dried in a vacuum freeze dryer to obtain blueberry anthocyanin powder. 2. The method for rapidly extracting anthocyanins from blueberry pomace according to claim 1, wherein in the step (4), the filter mesh of the centrifuge is 200 meshes, and the decompression rotary evaporation device The temperature for concentration under reduced pressure was 45 °C. 3 . The method for rapidly extracting anthocyanins from blueberry pomace according to claim 1 , wherein in the steps (1)-(5), the temperature of the feed liquid is controlled below 50° C. 4 .