CN106665571B - Improve the biological agent and its preparation method and application of turf grass drought resistance - Google Patents
Improve the biological agent and its preparation method and application of turf grass drought resistance Download PDFInfo
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- CN106665571B CN106665571B CN201611053262.XA CN201611053262A CN106665571B CN 106665571 B CN106665571 B CN 106665571B CN 201611053262 A CN201611053262 A CN 201611053262A CN 106665571 B CN106665571 B CN 106665571B
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- ethionine
- drought resistance
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- turf grass
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses the biological agents and its preparation method and application for improving turf grass drought resistance.Ethionine is improving the application in turf grass drought resistance.A kind of biological agent improving turf grass drought resistance, takes water as a solvent, includes 50-350mg/L ethionine.The preparation method of the biological agent of the present invention for improving turf grass drought resistance comprising the steps of: 1) weigh ethionine and be added to the water, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；2) Tween-20 is weighed, is put into the solution of step 1) preparation, is configured to the solution of volume ratio 1 ‰, is sufficiently stirred on magnetic stirring apparatus and is allowed to uniform.Ethionine can be improved the drought resistance of turfgrass, and under drought stress environment, the turfgrass for spraying ethionine can enhance plant moisture holding capacity, maintain higher turf quality.
Description
Technical field
The invention belongs to sod production and management technique field, be related to improving the biological agent of turf grass drought resistance and
Preparation method and application.
Background technique
Lawn is mitigating city haze, absorption toxic and harmful gas, is reducing fugitive dust, conserves water and soil, alleviates visual fatigue etc.
Aspect has played important function.Ended for the end of the year 2014, the Chinese city greenery patches gross area has reached 252.79 ten thousand hm2(China statistical
Yearbook, 2015), and green coverage is continuing to grow at top speed.However, China's water resource critical shortage, urban afforestation water are special
Be lawn irrigation water and production and living water contradiction it is also outstanding day by day, make adverse effect of the turfgrass by water deficit,
Including the symptoms such as leaf abscission, wilting, chlorosis, turf quality decline, especially in arid and semi-arid area, these influences are more bright
It is aobvious.Even if period of short-term drought also will affect the landscape and ecological functions of turfgrass in humid region.Therefore, turfgrass is effectively improved
Drought resistance is to solve urban lawn greening urgent problem to be solved, may advantageously facilitate the development of China's Lawn Industry, has huge
Economic value and vast potential for future development.
Currently, the methods of wild species introduction and acclimatization, genetic engineering breeding, but these can be used by improving turf grass drought resistance
Method is time-consuming long, at high cost, needs to put into a large amount of manpower and material resources.In addition to the above measure, squirting biological agent by external source is one
The important channel of quick, the effective and easy to spread raising turf grass drought resistance of kind.
Ethionine (ethionine) is a kind of sulfur-containing amino acid, molecular weight 163.24, in plant field with relatively
More is mutant screening to be carried out as mutagens, but yet there are no report as the drought resistance aspect that biological agent improves turfgrass
Road.
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide the new opplications of ethionine.
It is a further object of the present invention to provide a kind of biological agents for improving turf grass drought resistance.
It is yet another object of the invention to provide the preparation methods of said preparation.
The purpose of the present invention can be achieved through the following technical solutions:
Ethionine is improving the application in turf grass drought resistance.
A kind of biological agent improving turf grass drought resistance, takes water as a solvent, includes 25-350mg/L ethionine, preferably
Contain 50-300mg/L ethionine；Further preferably 50mg/L ethionine or 250mg/L ethionine.To season type
Grass, ethionine optium concentration are 50mg/L, are 250mg/L to cool-season grasses optium concentration.
Wherein, the ethionine is L- ethionine.
In the preparation further preferably containing 1 ‰ Tween-20.
The preparation method of the biological agent of the present invention for improving turf grass drought resistance comprising the steps of:
1) it weighs ethionine to be added to the water, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；
2) Tween-20 is weighed, is put into the solution of step 1) preparation, the solution of volume ratio 1 ‰ is configured to, in magnetic agitation
It is sufficiently stirred on device and is allowed to uniform.
Biological agent of the present invention is improving the application in turf grass drought resistance.
The utility model has the advantages that
Ethionine can be improved the drought resistance of turfgrass, under drought stress environment, spray the turfgrass of ethionine
Plant moisture holding capacity can be enhanced, maintain higher turf quality.Foliage-spray ethionine inhibits the mistake of turfgrass film rouge
Oxidation, maintains the stability and integrality of higher cell membrane, is embodied in lower EL and MDA content；And make CAT
It is significantly increased with the plant under APX activity ratio individually arid.
Detailed description of the invention
Fig. 1 experiment process 15 days, relief capabilities of the various concentration ethionine to cold-season turfgrass drought stress
Relief capabilities of Fig. 2 various concentration ethionine to warm season turf drought stress
Soil moisture content under Fig. 3 different disposal
Control is control, and ethionine is ethionine, and Drought is Osmotic treatment, similarly hereinafter
The phenotypic map of Festuca Arundinacea under Fig. 4 Osmotic treatment
Influence of Fig. 5 different disposal to turf quality
Note: vertical line indicates least significant difference LSD, the level of signifiance P=0.05 between different disposal in figure, similarly hereinafter.
Influence of Fig. 6 different disposal to Festuca Arundinacea leaf water content
Influence of Fig. 7 different disposal to turbine blades in electrochemical matter percolation ratio and mda content
Different lowercases represent significant difference between different disposal on the same day, similarly hereinafter.
Influence of Fig. 8 different disposal to antioxidant enzyme activities in leaves
Specific embodiment
Embodiment 1
1, the preparation of biological agent
1) it weighs ethionine to be added to the water, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；
2) Tween-20 (Tween-20) is weighed, is put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
It mixes to be sufficiently stirred on device and is allowed to uniform.
2, materials and methods
Material to be tested is cold-season turfgrass Festuca Arundinacea ' Arid3 ' (Festuca arundinacea cv. ' Arid3 '),
The method nourished and generated is taken to carry out material culture.Take on June 3rd, 2015 and generates within 2 years ripe tall grass skin bit, transplanting
Into the pvc pipe for filling complete husky matrix (the diameter 11cm, height 50cm of pipe), sand is rough sand in the washing of the Changjiang river.Turfgrass is in temperature
Preculture in the greenhouse of room, every 2d water 1 time, are once trimmed every 2d according to 1/3 principle, and keeping height is about 5cm.Pre- training
Feeding period applies urea once (nitrogen content >=46.3%), pays attention to pre- disease prevention.Hygrothermograph is installed in greenhouse, record it is daily most
High temperature, the lowest temperature and relative humidity.
Material preculture 60 days, until growing fine, starting test process when uniform color is consistent.Ethionine set 6 it is dense
It spends gradient and carries out concentration screening, respectively 0mg/L, 50mg/L, 100mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/
L,350mg/L.Each concentration processing is repeated 5 times.0d starts to spray, and sprays once every 5d later, and coprocessing 15 days.
Using RANDOMIZED BLOCK DESIGNs, 8 processing are designed, are respectively:
(1) arid D: foliage-spray 10ml distilled water；
(2) arid D+ethionine ethionine: foliage-spray 10ml C1(50mg/L) ethionine；
(3) arid D+ethionine ethionine: foliage-spray 10ml C2(100mg/L) ethionine；
(4) arid D+ethionine ethionine: foliage-spray 10ml C3(150mg/L) ethionine；
(5) arid D+ethionine ethionine: foliage-spray 10ml C4(200mg/L) ethionine；
(6) arid D+ethionine ethionine: foliage-spray 10ml C5(250mg/L) ethionine；
(7) arid D+ethionine ethionine: foliage-spray 10ml C6(300mg/L) ethionine；
(8) arid D+ethionine ethionine: foliage-spray 10ml C7(350mg/L) ethionine.
Osmotic treatment group (1)-(8) stop watering completely, carry out note of taking pictures when testing 0d, 5d, 10d, 15d
Record, every pipe spray isometric distilled water or ethionine solution 10ml, require atomization good when spraying, and uniformly, water droplet is or not sprinkling
At under plume.
3, result
As seen from Figure 1, Osmotic treatment 15 days when, the apparent mass of Festuca Arundinacea ' Arid3 ' occurs under different disposal
It is decreased obviously, but the processing of foliage-spray 250mg/L ethionine has preferable level ground appearance quality, and it is best to hold green character.
Embodiment 2
1, the preparation of biological agent
1) ethionine is weighed, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；
2) Tween-20 (Tween-20) is weighed, is put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
It mixes to be sufficiently stirred on device and is allowed to uniform.
2, materials and methods
Material to be tested is that (Cynodon dactylon cv. ' Tifway ', takes nutrition numerous to warm season turf Bermuda grass
The method grown carries out material culture.In on July 4th, 2015 to the aerial stem of plant cuttage of acquisition into the pvc pipe for filling complete husky matrix
(the diameter 11cm, height 50cm of pipe).Sand is rough sand in the washing of the Changjiang river.Turfgrass preculture in greenhouse, every 2d watering 1
It is secondary, it is once trimmed every 2d according to 1/3 principle, keeping height is about 5cm.Primary (the nitrogen content of urea is applied during preculture
>=46.3%), pay attention to pre- disease prevention.Hygrothermograph is installed in greenhouse, the daily highest temperature, the lowest temperature and relatively wet are recorded
Degree.
Material preculture 60 days, until growing fine, starting test process when uniform color is consistent.Ethionine is set, and 4 dense
It spends gradient and carries out concentration screening, respectively 0mg/L, 25mg/L, 50mg/L, 150mg/L.Each concentration processing is repeated 5 times.0d is opened
Beginning sprays, and sprays once every 5d later, and coprocessing 25 days.
Using RANDOMIZED BLOCK DESIGNs, 4 processing are designed, are respectively:
(1) arid D: foliage-spray steams 10ml distilled water；
(2) arid D+ethionine ethionine: foliage-spray 10ml C1(25mg/L) ethionine；
(3) arid D+ethionine ethionine: foliage-spray 10ml C2(50mg/L) ethionine；
(4) arid D+ethionine ethionine: foliage-spray 10ml C3(150mg/L) ethionine.
Osmotic treatment group (1)-(4) stop watering completely, carry out note of taking pictures when testing 0d, 5d, 10d, 15d
Record, every pipe spray isometric distilled water or ethionine solution 10mL, require atomization good when spraying, and uniformly, water droplet is or not sprinkling
At under plume.Control group replaces medical fluid foliage-spray with equal amount of distilled water.
3, result
As seen from Figure 2, Osmotic treatment 20 days when, under different disposal the apparent mass of Bermuda grass occur it is obvious under
Drop, but the processing of foliage-spray 50mg/L ethionine has preferable level ground appearance quality, and it is best to hold green character.
Embodiment 3
1, the preparation of biological agent
1) ethionine is weighed, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；
2) Tween-20 (Tween-20) is weighed, is put into 1) solution, the solution of 1 ‰ (volume ratios) is configured to, is stirred in magnetic force
It mixes to be sufficiently stirred on device and is allowed to uniform.
2, materials and methods
Material to be tested is Festuca Arundinacea ' Arid3 ' (F.arundinacea cv. ' Arid3 '), takes the method nourished and generated
Carry out material culture.Take on June 3rd, 2,015 2 years and generate ripe tall grass skin bit, is transplanted to and fills complete husky matrix
In pvc pipe (the diameter 11cm, height 50cm of pipe).Sand is rough sand in the washing of the Changjiang river.Turfgrass preculture in greenhouse, often
2d waters 1 time, is once trimmed every 2d according to 1/3 principle, and keeping height is about 5cm.It is primary that urea is applied during preculture
(nitrogen content >=46.3%) pays attention to pre- disease prevention.Hygrothermograph is installed in greenhouse, records the daily highest temperature, the lowest temperature
And relative humidity.
Material preculture 60 days, until growing fine, starting test process when uniform color is consistent.Ethionine concentration uses
The 250mg/L that embodiment 1 filters out.Each processing is repeated 5 times, and coprocessing 25 days.
Using RANDOMIZED BLOCK DESIGNs, 4 processing are designed, are respectively:
(1) Control is compareed: normal watering；
(2) Control+ethionine ethionine is compareed: normal watering, while foliage-spray 10ml 250mg/L second
Methyllanthionine；
(3) arid D: foliage-spray 10ml distilled water；
(4) arid D+ethionine ethionine: foliage-spray 10ml 250mg/L ethionine.
Osmotic treatment group (1)-(4) stop watering completely, take pictures when testing 0d, 5d, 10d, 18,25d
Record, evaluate turf quality (Turf quality, TQ), and measure soil water content (Soil water content,
SWC), leaf r elative water content (Relative water content, RWC), Electrolytic leakage (Electrolyte
leakage,EL).In 25 days measurement malonaldehyde (MDA) contents of experiment process and activities of antioxidant enzymes, including superoxide dismutase
Enzyme (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase enzyme (APX).Every pipe sprays
Volume distilled water or ethionine solution 10ml require atomization good when spraying, uniformly, water droplet is not under plume for sprinkling.
3, result
From figure 3, it can be seen that SWC is not significant between two processing either in normal watering or under arid situation
Difference illustrates that the soil moisture situation for compareing positive Osmotic treatment is respectively at same level.
As can be seen from Figure 4 and Figure 5, Osmotic treatment reduces Turf Quality of Tall Fescue constantly, when coercing 18 days, lawn matter
Amount sharply falls to the 30% of control, but foliage-spray ethionine maintains higher turf quality, turf quality only under
It is reduced to the 13% of control, and is statistically not significantly different.When handling 25 days, two controls all maintain 7.8-8, but coerce
Urgent processing is remarkably decreased turf quality, and arid makes 45% that it falls to control, and foliage-spray ethionine makes grass
Level ground quality falls to the 24% of control, illustrates that foliage-spray ethionine can significantly improve the drought resisting energy of cold-season turfgrass
Power.
Leaf water content RWC is one of the important indicator for reacting plant drought resistance, directly represents the energy that blade saves moisture
Power.From fig. 6, it can be seen that individually Osmotic treatment 5 days when, Festuca Arundinacea RWC is just remarkably decreased, but in whole experiment process
In, foliage-spray ethionine makes RWC be significantly higher than the plant of independent arid, illustrates that ethionine can be improved plant water holding
Ability.
Electrolytic leakage EL is capable of the stability and integrality of reacting cells film, and MDA can embody the peroxide of film rouge
Change situation.From figure 7 it can be seen that degree of drought constantly aggravates with the extension of dry spell, EL and MDA are significantly increased, and leaf
Face sprays the peroxidating that ethionine inhibits film rouge, maintains the stability and integrality of higher cell membrane, specific manifestation
For lower EL and MDA content.
Arid can cause the accumulation of active oxygen (ROS), and plant is self by starting Antioxidant Enzyme Systems elimination activity
Destruction of the oxygen to film, to improve the drought-resistant ability of plant.From figure 8, it is seen that independent arid is drawn with the extension of experiment periods
The active significant raising of SOD and POD is played, foliage-spray ethionine does not influence the activity of SOD enzyme, but keeps POD enzymatic activity big
Width rises, and is 1.43 times under independent arid situation；Meanwhile under arid situation, foliage-spray ethionine makes CAT and APX
Plant under the independent arid of activity ratio significantly increases.
Claims (6)
1. ethionine is improving the application in turf grass drought resistance.
2. application according to claim 1, which is characterized in that take water as a solvent, be configured to the sulphur ammonia of second containing 25-350mg/L
The aqueous solution of acid and 1 ‰ Tween-20s is applied.
3. application according to claim 2, it is characterised in that ethionine concentration is 50-300 mg/L.
4. application according to claim 3, it is characterised in that ethionine concentration is 50 mg/L or 250 mg/L.
5. the application according to any one of claim 2-4, it is characterised in that the ethionine is L- second sulphur amino
Butyric acid.
6. a kind of preparation method for the biological agent for improving turf grass drought resistance, it is characterised in that comprise the steps of:
1) it weighs ethionine to be added to the water, stirring is allowed to sufficiently dissolve on magnetic stirring apparatus；
2) Tween-20 is weighed, is put into the solution of step 1) preparation, the solution of volume ratio 1 ‰ is configured to, on magnetic stirring apparatus
It is sufficiently stirred and is allowed to uniform.
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| CN1710076A (en) * | 2005-06-01 | 2005-12-21 | 林忠平 | Use of boea crassifolia BcBCP1 gene for breeding drought-salt-tolerant plants |
| CN101233860A (en) * | 2007-11-19 | 2008-08-06 | 吉林师范大学 | Preparation method and application of soybean drought-resistant and yield-increasing regulator |
| CN101899107A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院上海生命科学研究院 | Subregulatory effect and application of RACK1b gene on drought and salt tolerance of plants |
| CN102138560A (en) * | 2011-01-24 | 2011-08-03 | 广东省农业科学院土壤肥料研究所 | Organic nutritional drought-resistance agent preparation and preparation method thereof |
| CN104068039A (en) * | 2014-06-27 | 2014-10-01 | 何芳 | Pesticide composition for fruits and vegetables |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6419005A (en) * | 1987-07-15 | 1989-01-23 | Central Glass Co Ltd | Agent for promoting growth of mycelia and formation of fruit body of basidiomycetes |
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2016
- 2016-11-25 CN CN201611053262.XA patent/CN106665571B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710076A (en) * | 2005-06-01 | 2005-12-21 | 林忠平 | Use of boea crassifolia BcBCP1 gene for breeding drought-salt-tolerant plants |
| CN101233860A (en) * | 2007-11-19 | 2008-08-06 | 吉林师范大学 | Preparation method and application of soybean drought-resistant and yield-increasing regulator |
| CN101899107A (en) * | 2009-05-27 | 2010-12-01 | 中国科学院上海生命科学研究院 | Subregulatory effect and application of RACK1b gene on drought and salt tolerance of plants |
| CN102138560A (en) * | 2011-01-24 | 2011-08-03 | 广东省农业科学院土壤肥料研究所 | Organic nutritional drought-resistance agent preparation and preparation method thereof |
| CN104068039A (en) * | 2014-06-27 | 2014-10-01 | 何芳 | Pesticide composition for fruits and vegetables |
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Effective date of registration: 20170525 Address after: 211800, Nanjing Road, Jiangpu District, Jiangsu City, Pukou Province, Zhujiang Industrial Park, Rui Yun Road, 3-309 Applicant after: Nanjing Bio Technology Co., Ltd. Address before: 211800, Nanjing Road, Jiangpu District, Jiangsu City, Pukou Province, Zhujiang Industrial Park, Rui Yun Road, 3-309 Applicant before: Huang Bingru |
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