CN106665436A - Method of transplanting healthy fish intestinal feces and treating ill fishes - Google Patents
Method of transplanting healthy fish intestinal feces and treating ill fishes Download PDFInfo
- Publication number
- CN106665436A CN106665436A CN201710061602.1A CN201710061602A CN106665436A CN 106665436 A CN106665436 A CN 106665436A CN 201710061602 A CN201710061602 A CN 201710061602A CN 106665436 A CN106665436 A CN 106665436A
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- Prior art keywords
- fish
- filter
- fishes
- healthy
- ill
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 96
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 25
- 210000003608 fece Anatomy 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 15
- 239000000243 solution Substances 0.000 claims abstract description 11
- 208000035143 Bacterial infection Diseases 0.000 claims abstract description 9
- 230000004083 survival effect Effects 0.000 claims abstract description 9
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 210000000936 intestine Anatomy 0.000 claims description 10
- 241000252233 Cyprinus carpio Species 0.000 claims description 8
- 241000252234 Hypophthalmichthys nobilis Species 0.000 claims description 8
- 208000022362 bacterial infectious disease Diseases 0.000 claims description 8
- 241000252229 Carassius auratus Species 0.000 claims description 7
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 7
- 241000720946 Hypophthalmichthys molitrix Species 0.000 claims description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 4
- 235000021060 food property Nutrition 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 11
- 230000036541 health Effects 0.000 abstract description 10
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 238000001914 filtration Methods 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 235000018291 probiotics Nutrition 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a method of transplanting healthy fish intestinal feces and treating ill fishes. The method comprises the steps of S1, collecting healthy fish intestinal feces, adding the collected feces into stroke-physiological saline solution for dissolution, stirring the solution evenly, filtering the solution, placing the filtered healthy feces bacteria fluid into a vessel, S2, adding filter-feeding fishes in the vessel, wherein the mass of each fish is about 50 g, the adding quantity is 10-20 fishes per cubic meter of water body; supplementing oxygen into the water to keep the filter-feeding fishes alive, breeding the filter-feeding fishes in the vessel for 10-20 days continuously, S3, adding fishes inflicted with intestinal bacterial diseases into the vessel based on the quantity of 5-10 fishes per cubic meter of water body, and breeding for 10-20 days. According to the method of transplanting healthy fish intestinal feces and treating ill fishes, the transplantation of health fish intestinal bacteria can improve intestinal microbiome of the ill fishes, the survival rate of the ill fishes are obviously increased, and therefore the method is applicable for large scale application.
Description
Technical field
The present invention relates to the side of the ill fish of enteron stool treatment of fish culture field, the especially a kind of healthy fish of transplanting
Method.
Background technology
In recent years, the research with regard to human body intestinal canal microorganism has become a focus, and many researchs find human body intestinal canal
Microorganism is closely related with human diseasess, health and growth etc..Human body intestinal canal microorganism group plan is considered as second gene
Group plan.Compared with human intestinal microorganisms study, the enteric microorganism researches of other animals.
The research of fish intestines microorganism is also in the starting stage now, with regard to its enteric microorganism composition, species and fish
The correlation researchs such as growth itself, nutrition, immunity are also less.At present, researcher is by the addition battalion in fish feed
The modes such as foster material, immunostimulant, Chinese herbal medicine improve the immunity of Fish body promoting the growth of Fish.But also do not have
Have by using other healthy fish intestines floras to change itself intestinal microbial population, to reach raising survival rate, treatment is with thin
The method of bacterium property disease fish.Because the method for traditional utilization intestinal bacteria treatment is solid form delivery, and Fish are lived in water,
Fish feed is thrown in in water, has a significant difference with existing mode, and solid form delivery does not apply to Fish, and current no liquid
The record of administration.So being badly in need of now a kind of intestinal by transplanting the enteron stool (flora) of healthy Fish to ill Fish
In, to change ill fish intestines Bacterial community, so as to the method for improving Fish survival rate, increasing its immunity of organisms.
The content of the invention
The invention aims to solve in prior art, temporarily nothing treats ill Fish by healthy fish intestines bacterium
Method problem, and then provide a kind of by transplanting healthy method of the fish enteron stool to treat ill fish.
The technical scheme is that：Temporarily slightly
The present invention has the advantages that：The method of the ill Fish for the treatment of of the present invention, is by transplanting healthy Fish
Intestinal is realizing.Will suffer from bacterial disease fish such as Cyprinus carpio, Ctenopharyngodon idellus, Carassius auratuss etc., be placed at the same add health Fish with
Cultivate in environment containing intestinal bacterium solution, through about 10-20 days, it can be found that the mortality rate of wherein ill fish is substantially reduced.
The healthy recovery of Fish and its intestinal bacterium solution to ill fish of this explanation, serves conclusive effect so that the intestinal of ill fish
Into being improved, its survival rate is significantly improved microorganism group, is greatly lowered treatment by shirtsleeve operation
The difficulty of ill Fish.And this method low cost, it is suitable to large-scale promotion application.
Specific embodiment
A kind of healthy fish enteron stool of transplanting of the present invention mainly comprises the steps in the method for treating ill fish：
Step one, the healthy fish intestines feces of collection, dissolve in being added to physiological saline solution, filter after stirring,
Healthy feces bacterium solution after by filter is placed in container；
Step 2, filter-feeding fish is added in the above-described container, addition weight is 50g, and quantity is 10~20 tails/m3Water
Body, oxygenation are persistently cultivated 10~20 days in the above-described container with keeping filter-feeding fish health survival, filter-feeding fish；
Step 3, will suffer from intestinal bacteriosis Fish according to 5~10 tails/m3The quantity of water body is placed in the container
In, cultivate 10~20 days.
Operation in above-mentioned steps one is carried out in an aseptic environment；Collect side of the healthy fish intestines feces using siphon
The ratio of method, fish intestines feces and physiological saline solution is 1:10.
In above-mentioned steps two, filter-feeding fish is healthy spotted silver carp or Hypophthalmichthys molitrix, the additional proportion of fish be 10~20 tails/
m3Water body, fish are persistently cultivated 10~20 days or so in a reservoir.Optimal culturing time is 15 days.
In step 3, the Fish with bacterial disease are Cyprinus carpio, Ctenopharyngodon idellus, Carassius auratuss etc., according to 5~10 tails/m3Water body is put
In the container, cultivate 10~20 days.It is 2 that filter-feeder fish throws in mantissa's ratio with the fish with intestinal bacteriosis:1.
The filter-feeding fish of health is added in step 2, the microorganism (probioticss) in healthy feces bacterium solution is by filter-feeder fish
Class is eaten and enters intestinal, continues breeding, subsequently drain in container water body, so as to increase in the filter-feeding fish intestinal of health
The quantity of probioticss in water body, and ensure that the activity of probioticss.The Excreta of healthy filter-feeding fish is simultaneously in water body
Probioticss existence is provided and is bred needed for nutrient substance, further improve the quantity of probioticss, concentration and vigor in water body.
With reference to specific embodiment, the present invention will be further described：
Specific embodiment one
The method of the present invention comprises the steps：Step one, using the method for siphon, fish faeces are collected in aseptic appearance
In device, addition physiological saline solution is dissolved, and the ratio of fish intestines feces and sterilized water is 1:10；By fish of the dissolving after good
Enteron stool mixture, is filtered by aseptic common qualitative filter paper, filters solid residue, and the liquid after filtration is placed in one in advance
In the open square groove for first sterilizing, the square groove is set according to the size of concrete fish；In square groove, the amount of added bacterium solution is not having
Cross it is required be put into the size of fish depending on, can not have fish；
Step 2, add filter-feeding Aristichthys nobiliss or Hypophthalmichthys molitrix in square groove, additional proportion is 10~20 tails/m3Water body,
Oxygenation is persistently cultivated 10~20 days in a reservoir with keeping spotted silver carp health survival, filter-feeder fish；
Step 3, by with the Cyprinus carpio of bacterial disease, Ctenopharyngodon idellus, Carassius auratuss etc., according to 5~10 tails/m3Water body is placed in square
In groove, cultivate 10~20 days.
Specific embodiment two
Implementation comprises the steps：It is step one, identical with specific embodiment one；
Step 2, by with the Cyprinus carpio of bacterial disease, Ctenopharyngodon idellus, Carassius auratuss according to 5~10 tails/m3Water body is placed in square groove
In, cultivate 10~20 days.
Specific embodiment three
Implementation comprises the steps：Step one, selection and size identical square groove in specific embodiment one, plus
Enter filter-feeding Aristichthys nobiliss or Hypophthalmichthys molitrix, additional proportion is 10~20 tails/m3Water body, oxygenation are filtered with keeping spotted silver carp health survival
Feeding habits fish persistently cultivates 10~20 days in a reservoir；
Step 2, by with the Cyprinus carpio of bacterial disease, Ctenopharyngodon idellus, Carassius auratuss according to 5~10 tails/m3Water body is placed in square groove
In, cultivate 10~20 days.
Specific embodiment four
Implementation comprises the steps：Choose and size identical square groove in specific embodiment one, addition is suffered from
The Cyprinus carpio of bacterial disease, Ctenopharyngodon idellus, Carassius auratuss are according to 5~10 tails/m3Water body is placed in square groove, is cultivated 10~20 days.
Experiment effect
The present invention adopts following experimental verification effect：
480 tail of ill Cyprinus carpio is chosen, 4 groups is classified as, respectively according to the method for above-mentioned specific embodiment one to four
Operation, per group make again 3 it is parallel according to group, each according to group place a 40 tail fishes.Concrete packet situation see the table below 1.
Table 1
By the cultivation of 15 days or so, it is found that the ill fish in group 1 is dead minimum, and ill fish starts to recover, group 2
The effects for only adding 3 addition spotted silver carp of health bacterium and group are close, but significantly lower than group 1, and in the case that group 4 is no added,
Spotted silver carp mortality.
This explanation is in identical breeding environment, while adding the recovery of healthy Fish and its intestinal bacterium solution to ill fish to rise
Conclusive effect is arrived.If only adding the bacterium solution of healthy fish, increase over time, bacterial concentration can be gradually lowered, treatment
Effect also declines therewith.If only adding healthy fish, the concentration in water which voids excreta is relatively low always, and what is do not had controls
Therapeutic effect.Both are mixed and are added to the water by the present invention, on the bacterial concentration basis added, by the continuous row of healthy fish
Just, the concentration of bacterium solution is maintained in higher level, and will not be declined with the time, thus make the enteric microorganism of ill fish
Composition has obtained basic improvement, and its survival rate is significantly improved, and greatly reduces the difficulty of the ill Fish for the treatment of.
The above is only presently preferred embodiments of the present invention, is not intended to limit embodiment of the present invention, and this area is general
Logical technical staff central scope of the invention and spirit, can very easily carry out corresponding flexible or modification, therefore this
The protection domain of invention should be defined by the protection domain required by claims.
Claims (9)
1. it is a kind of to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that to comprise the steps：
Step one, the healthy fish intestines feces of collection, dissolve in being added to physiological saline solution, filter after stirring, will filter
Healthy feces bacterium solution afterwards is placed in container；
Step 2, filter-feeding fish is added in the above-described container, addition quantity is 10~20 tails/m3Water body, oxygenation are eaten with keeping filter
Property healthy fish survival, filter-feeding fish in the above-described container persistently cultivate 10~20 days；
Step 3, will suffer from intestinal bacteriosis Fish according to 5~10 tails/m3Quantity be placed in the container, cultivation 15
~20 days.
2. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that described
In step one, filter-feeding fish is spotted silver carp or Hypophthalmichthys molitrix.
3. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
The ratio of one Mesichthyes enteron stool and physiological saline solution is 1:10.
4. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
Method of the healthy fish intestines feces using siphon is collected in one.
5. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
Operation in one is carried out in an aseptic environment.
6. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
In two, the additional proportion of filter-feeding fish is 10~20 tails/m3Water body, filter-feeding fish persistently cultivate 10~20 in a reservoir
My god.
7. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
Ill Fish in three should be the Cyprinus carpio with bacterial disease, Ctenopharyngodon idellus or Carassius auratuss.
8. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step
The Fish with bacterial disease in three are according to 5~10 tails/m3Water body is placed in the container, is cultivated 10~20 days.
9. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that filter food
Property fish to throw in mantissa ratio with the fish with intestinal bacteriosis be 2:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710061602.1A CN106665436A (en) | 2017-01-26 | 2017-01-26 | Method of transplanting healthy fish intestinal feces and treating ill fishes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710061602.1A CN106665436A (en) | 2017-01-26 | 2017-01-26 | Method of transplanting healthy fish intestinal feces and treating ill fishes |
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| Publication Number | Publication Date |
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| CN106665436A true CN106665436A (en) | 2017-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710061602.1A Pending CN106665436A (en) | 2017-01-26 | 2017-01-26 | Method of transplanting healthy fish intestinal feces and treating ill fishes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112970977A (en) * | 2021-04-02 | 2021-06-18 | 天津农学院 | Feed additive for promoting intestinal microecological recovery of fish, preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103561752A (en) * | 2011-03-09 | 2014-02-05 | 明尼苏达大学评议会 | Compositions and methods for transplantation of colon microbiota |
| CN203694209U (en) * | 2013-12-03 | 2014-07-09 | 上海市第十人民医院 | FMT (Fecal Microbiota Transplantation) fecal treatment device |
| CN104688780A (en) * | 2015-03-31 | 2015-06-10 | 徐高骁 | Method for preventing and treating piglet diarrhea based on caprophyl implantation technique |
| CN104922158A (en) * | 2015-06-05 | 2015-09-23 | 中国人民解放军第三军医大学第三附属医院 | Fecal microbiota capsule, as well as preparation method and application thereof |
| CN105385599A (en) * | 2016-01-21 | 2016-03-09 | 南京医科大学第一附属医院 | Coprophilous fungus preservation solution and method for preserving coprophilous fungi |
| CN106714553A (en) * | 2014-09-05 | 2017-05-24 | 日本曹达株式会社 | Fishhook |
-
2017
- 2017-01-26 CN CN201710061602.1A patent/CN106665436A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103561752A (en) * | 2011-03-09 | 2014-02-05 | 明尼苏达大学评议会 | Compositions and methods for transplantation of colon microbiota |
| CN203694209U (en) * | 2013-12-03 | 2014-07-09 | 上海市第十人民医院 | FMT (Fecal Microbiota Transplantation) fecal treatment device |
| CN106714553A (en) * | 2014-09-05 | 2017-05-24 | 日本曹达株式会社 | Fishhook |
| CN104688780A (en) * | 2015-03-31 | 2015-06-10 | 徐高骁 | Method for preventing and treating piglet diarrhea based on caprophyl implantation technique |
| CN104922158A (en) * | 2015-06-05 | 2015-09-23 | 中国人民解放军第三军医大学第三附属医院 | Fecal microbiota capsule, as well as preparation method and application thereof |
| CN105385599A (en) * | 2016-01-21 | 2016-03-09 | 南京医科大学第一附属医院 | Coprophilous fungus preservation solution and method for preserving coprophilous fungi |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112970977A (en) * | 2021-04-02 | 2021-06-18 | 天津农学院 | Feed additive for promoting intestinal microecological recovery of fish, preparation method and application |
| CN112970977B (en) * | 2021-04-02 | 2022-03-22 | 天津农学院 | Feed additive for promoting intestinal microecological recovery of fish, preparation method and application |
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Application publication date: 20170517 |
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