CN106645154A - Enrichment and observation apparatus and method for parasite eggs - Google Patents

Abstract

The invention provides a device and method for enrichment and observation of parasite eggs, including a thin film chip and a slide table, the thin film chip is composed of a base and a transparent film whose edge is sealed on the base, and a liquid storage chamber is formed between the transparent film and the base There is a guide post in the slide table, a slider is installed on the guide post, a handle is provided above the slider, and a slide is provided below. The distance between the bottom of the slide and the base is smaller than the distance between the highest point of the liquid storage chamber and the base. The thin-film chip is equipped with height-adjustable feet; the sample to be tested is mixed by moving the slider left and right, and then through the specific combination of magnetic beads and parasite eggs, and under the action of a magnetic field, the parasite eggs are placed on the chip. Enrichment and purification are convenient for subsequent microscopic detection. The present invention is also applicable to the enrichment of parasite eggs in feces, urine and other excreta. Through the above-mentioned device and method of use, the present invention not only realizes the specific enrichment operation of parasite eggs, but also can be directly used for microscopic observation , easy to operate and easy to automate.

Description

A device and method for enrichment and observation of parasite eggs

technical field

The invention relates to the technical field of biological experiment equipment, and more specifically, to a device and method for enriching and observing parasite eggs.

Background technique

In the field of diagnosis of parasitic diseases, the gold standard for clinical diagnosis is to extract and find parasite eggs from the host or host excreta. For example, for the detection of schistosomiasis ravaging in the waters of Dongting Lake in the Yangtze River, the classic detection method is to detect fecal eggs (also known as Kato-Katz method). However, the traditional method of extracting insect eggs from feces generally involves steps such as homogenization, filtration, washing, and smearing. Not only is the operation cumbersome and time-consuming, the extraction efficiency is low, and it can easily lead to false negative detection and environmental pollution.

In view of the above problems, there have been related technologies or devices to improve the efficiency of extracting eggs from feces. Most of these technologies or devices focus on the homogenization and dilution of feces, so as to improve the extraction efficiency of eggs. However, the whole feces inspection process also involves steps such as filtering, washing, and smearing, and these steps still require independent instruments or equipment for auxiliary operations to complete the entire feces inspection process. At the same time, the traditional method has a certain loss in the enrichment, purification and smear operation after the separation of eggs and feces, resulting in low detection sensitivity of the traditional method. Facing the above-mentioned deficiencies, there is an urgent need for an integrated device for the purification, enrichment and detection of parasite eggs that is easy to automate and highly sensitive in clinical testing.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the above-mentioned defects in the prior art, and provide an observation device for enrichment of parasite worm eggs.

The second object of the present invention is to provide a method for enriching and observing parasite eggs by using the device.

The purpose of the present invention is achieved through the following technical solutions:

A device for enriching and observing parasite eggs, comprising a thin-film chip and a sliding platform located above the thin-film chip, the thin-film chip is composed of a base and a transparent film whose edges are sealed on the base, and a liquid storage is formed between the transparent film and the base The cavity, the slide table is provided with horizontal and parallel guide posts, the guide posts are equipped with sliders that can slide along the guide posts, the upper part of the slider is provided with a handle, and the lower part is provided with a slide piece, so The distance between the bottom end of the sliding sheet and the base is smaller than the distance between the highest point of the liquid storage cavity and the base, and the thin film chip is provided with height-adjustable legs.

The working principle of the device of the present invention: add the sample solution to be tested and the magnetic particle solution into the liquid storage cavity, and at the same time slide the sliding plate to make the solution in the liquid storage cavity fully mixed, and place the magnet at one end of the thin film chip, at this time The complex of magnetic particles and parasite eggs is specifically combined, the height of the feet is increased, and the slider is moved slowly so that the slide stays at a position that just isolates the complex of magnetic particles and parasite eggs; finally, the whole egg is observed The device can be observed on a microscope.

Because the shell of parasite eggs contains iron-phosphorus complexes, but this complex is not paramagnetic and cannot be directly controlled by external magnetism, but it can attract and combine with magnetic particles to form a complex, which can indirectly control parasites Egg enrichment operation. Especially for schistosome eggs and other shells rich in iron-phosphorus complexes, the enrichment effect is better.

In order to conveniently add the sample to be tested and the magnetic particle solution into the thin film chip, preferably, the substrate is provided with a through hole, and a plug is installed on the through hole, and more preferably, the plug is an elastic plug.

For easier observation, preferably, the substrate is a glass substrate; the thin film chip is made of colorless and transparent material.

In order to facilitate operation, the handle protrudes from the sliding platform; supporting legs are also provided on both sides of the sliding platform.

The present invention also provides a method for observing parasite eggs using the parasite egg observation device, comprising the following steps:

S1. Add the sample solution to be tested into the thin film chip, and add the magnetic particle solution at the same time to ensure that the combined volume of the sample solution to be tested and the magnetic particle solution does not exceed two-thirds of the volume of the storage cavity;

S2. Move the slider to one side of the slide table through the handle, and slide the handle back and forth to fully mix the solution in the film chip;

S3. Place the magnet under one side of the film chip, and slide the handle at the same time, waiting for the complex of magnetic particles and parasite eggs to be enriched in the area close to the magnet;

S4. Increase the height of the support feet, and slowly move the slider so that the slider stays at a position that just isolates the complex of magnetic particles and parasite eggs;

S5. Put the whole egg observation device on the microscope for observation.

Compared with the prior art, the present invention has the following beneficial effects:

The invention provides a device and method for enriching and observing parasite eggs, comprising a thin film chip and a sliding platform located above the thin film chip, the thin film chip is composed of a base and a transparent film whose edges are sealed on the base, and the transparent film and A liquid storage cavity is formed between the bases, and horizontal and parallel guide posts are arranged in the slide table, and a slider that can slide along the guide post is installed on the guide post, a handle is provided on the top of the slide block, and a A slide is provided, the distance between the bottom of the slide and the base is smaller than the distance between the highest point of the liquid storage cavity and the base, and the thin film chip is provided with height-adjustable legs; The sample is mixed, and then through the specific combination of magnetic beads and parasite eggs, and under the action of a magnetic field, the enrichment and purification of parasite eggs on the chip is realized, which is convenient for subsequent microscopic detection. The invention is also applicable to the enrichment of parasite eggs in feces, urine and other excrements, especially schistosome eggs, because the outer shell of schistosome eggs is rich in iron and is more firmly combined with magnetic particles. Therefore, the present invention not only realizes the extraction of parasite eggs in complex samples, but also the enrichment results can be directly used for clinical morphological observation and diagnosis.

Description of drawings

Fig. 1 is a schematic diagram (perspective) of the three-dimensional structure of the enrichment observation device described in Example 1;

Fig. 2 is a side sectional view of the enrichment observation device described in Example 1;

3 is a schematic diagram of the mixing operation of the sample to be tested by using the enrichment and observation device described in Example 1.

Fig. 4 is a schematic diagram of the mixing operation of the sample to be tested and the magnetic particle solution by using the enrichment observation device described in Example 1.

5 is a schematic diagram of the operation of magnetic enrichment using the enrichment observation device described in Example 1.

Explanation of reference signs: 1-sliding table; 2-handle; 3-guide column; 4-film chip; 5-transparent film; 6-plug; 7-slide; 8-slider; 9-magnet.

detailed description

The patent of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Among them, the accompanying drawings are for illustrative purposes only, showing only schematic diagrams rather than physical drawings, and should not be understood as limitations on this patent; in order to better illustrate the embodiments of the patent of the present invention, some parts of the accompanying drawings will be omitted , enlargement or reduction, does not represent the size of the actual product; for those skilled in the art, it is understandable that some known structures and their descriptions in the drawings may be omitted.

Example 1

An observation device for parasite eggs, comprising a thin film chip 4 and a slide table 1 positioned above the thin film chip 4, the thin film chip 4 is composed of a base and a transparent film 5 whose edges are sealed on the base, and the transparent film 5 is connected to the base A liquid storage cavity is formed between them. The slide table 1 is provided with horizontal and parallel guide pillars 3. The guide pillars 3 are equipped with a slider 8 that can slide along the guide pillars 3. Above the slider 8, a There is a handle 2, and a sliding piece 7 is arranged below, the distance between the bottom end of the sliding piece 7 and the base is smaller than the distance between the highest point of the liquid storage chamber and the base, and the thin film chip 4 is provided with height-adjustable legs.

The working principle of the device of the present invention is as follows: add the sample solution to be tested and the magnetic particle solution into the liquid storage cavity, and at the same time slide the sliding plate 7 to fully mix the solution in the liquid storage cavity, place the magnet 9 on one end of the thin film chip, At this time, the complex of magnetic particles and worm eggs is specifically combined, the height of the legs is increased, and the slider 8 is slowly moved, so that the slide 7 stays at a position that just isolates the complex of magnetic particles and parasite eggs; The whole insect egg observation device is placed on a microscope for observation.

In order to conveniently add the sample to be tested and the magnetic particle solution into the thin film chip 4, in this embodiment, the base is provided with a through hole, and a plug 6 is installed on the through hole, and the plug is an elastic plug.

For easier observation, in this embodiment, the substrate is a glass substrate; the thin film chip 4 is made of colorless and transparent material.

For the convenience of operation, the handle 2 protrudes outside the slide table 1; the two sides of the slide table 1 are also provided with supporting legs.

Example 2

The present invention also provides a method for observing parasite eggs using the parasite egg observation device described in Example 1, comprising the following steps:

S1. Add the sample solution to be tested into the thin film chip 4, and add the magnetic particle solution at the same time to ensure that the combined volume of the sample solution to be tested and the magnetic particle solution does not exceed two-thirds of the volume of the liquid storage cavity;

S2. Move the slider 8 to one side of the slide table 1 through the handle 2, slide the handle 2 back and forth, so that the solution in the film chip 4 is fully mixed;

S3. Place the magnet 9 under one side of the thin-film chip 4, and slide the handle 2 at the same time, waiting for the complex of magnetic particles and parasite eggs to be enriched in the area close to the magnet 9;

S4. Increase the height of the legs, and slowly move the slider 8, so that the slider 7 stays at a position that just isolates the composite of the magnetic particles and the parasite eggs;

S5. Put the whole egg observation device on the microscope for observation.

Claims (6)

1. An enrichment and observation device for parasite eggs, characterized in that it comprises a thin film chip (4) and a slide table (1) above the thin film chip (4), the thin film chip (4) consists of a base and an edge It is composed of a transparent film (5) sealed on the substrate. A liquid storage cavity is formed between the transparent film (5) and the substrate. The slide table (1) is provided with horizontal and parallel guide posts (3) to guide A slide block (8) that can slide along the guide post (3) is installed on the post (3), a handle (2) is provided above the slide block (8), and a slide piece (7) is provided below the slide block (8). The distance between the bottom end of the sheet (7) and the base is smaller than the distance between the highest point of the liquid storage chamber and the base, and the thin film chip (4) is provided with height-adjustable legs. 2. The device for enriching and observing parasite eggs according to claim 1, characterized in that, the base is provided with a through hole, and a plug (6) is installed on the through hole. 3. The enrichment and observation device for parasite eggs according to claim 1, characterized in that, the thin film chip is made of colorless and transparent material. 4. The device for enriching and observing parasite eggs according to claim 1, characterized in that, the handle protrudes outside the slide table. 5. The device for enriching and observing parasite eggs according to claim 1, characterized in that, supporting legs are provided on both sides of the slide table. 6. A method for observing parasite eggs using the parasite egg enrichment observation device according to any one of claims 1 to 5, characterized in that it comprises the following steps: S1. Add the sample solution to be tested into the thin film chip (4), and add the magnetic particle solution at the same time, ensuring that the combined volume of the sample solution to be tested and the magnetic particle solution does not exceed two-thirds of the volume of the liquid storage cavity; S2. Move the slider (8) to one side of the slide table (1) through the handle (2), and slide the handle (2) back and forth to fully mix the solution in the film chip (4); S3. Place the magnet (9) under one side of the film chip (4), and slide the handle (2) at the same time, waiting for the complex of magnetic particles and parasite eggs to be enriched in the area close to the magnet (9); S4. Increase the height of the feet, and slowly move the slider (8), so that the slider (7) stays at a position that just isolates the complex of magnetic particles and parasite eggs; S5. Put the whole egg observation device on the microscope for observation.