CN106620883A - Tissue-engineering three-dimensional porous bracket and manufacturing method thereof - Google Patents
Tissue-engineering three-dimensional porous bracket and manufacturing method thereof Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 title abstract 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 claims abstract description 63
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 63
- 239000010433 feldspar Substances 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 23
- 239000004927 clay Substances 0.000 claims abstract description 22
- 235000014653 Carica parviflora Nutrition 0.000 claims abstract description 21
- 244000132059 Carica parviflora Species 0.000 claims abstract description 21
- 229920000881 Modified starch Polymers 0.000 claims abstract description 21
- 239000004368 Modified starch Substances 0.000 claims abstract description 21
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 21
- 235000019426 modified starch Nutrition 0.000 claims abstract description 21
- 239000011259 mixed solution Substances 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 20
- 108010068370 Glutens Proteins 0.000 claims abstract description 19
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 19
- 235000021312 gluten Nutrition 0.000 claims abstract description 19
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 19
- 238000007710 freezing Methods 0.000 claims abstract description 15
- 230000008014 freezing Effects 0.000 claims abstract description 15
- 102000008186 Collagen Human genes 0.000 claims abstract description 14
- 108010035532 Collagen Proteins 0.000 claims abstract description 14
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 14
- 229930195725 Mannitol Natural products 0.000 claims abstract description 14
- 229920001436 collagen Polymers 0.000 claims abstract description 14
- 239000000594 mannitol Substances 0.000 claims abstract description 14
- 235000010355 mannitol Nutrition 0.000 claims abstract description 14
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000243 solution Substances 0.000 claims abstract description 11
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 19
- 235000012054 meals Nutrition 0.000 claims description 18
- 102000004407 Lactalbumin Human genes 0.000 claims description 15
- 108090000942 Lactalbumin Proteins 0.000 claims description 15
- 210000000988 bone and bone Anatomy 0.000 claims description 13
- 239000012467 final product Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 2
- 102000002322 Egg Proteins Human genes 0.000 claims description 2
- 108010000912 Egg Proteins Proteins 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 235000014103 egg white Nutrition 0.000 claims description 2
- 210000000969 egg white Anatomy 0.000 claims description 2
- 235000013336 milk Nutrition 0.000 claims description 2
- 239000008267 milk Substances 0.000 claims description 2
- 210000004080 milk Anatomy 0.000 claims description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 abstract 1
- 108010046377 Whey Proteins Proteins 0.000 abstract 1
- 102000007544 Whey Proteins Human genes 0.000 abstract 1
- 229940010747 sodium hyaluronate Drugs 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 235000021119 whey protein Nutrition 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 7
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 7
- 229920002674 hyaluronan Polymers 0.000 description 7
- 229960003160 hyaluronic acid Drugs 0.000 description 7
- 229910052708 sodium Inorganic materials 0.000 description 7
- 239000011734 sodium Substances 0.000 description 7
- 235000002917 Fraxinus ornus Nutrition 0.000 description 4
- 244000182067 Fraxinus ornus Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000011148 porous material Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3637—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
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Abstract
The invention provides a tissue-engineering three-dimensional porous bracket and a manufacturing method thereof. The manufacturing method comprises the following steps: (1) adding modified starch, sodium hyaluronate, collagen protein, soybean protein isolate, whey protein and vital gluten into twice weight of water which is heated to 45-90 DEG C, and sufficiently stirring for 0.5-3 hours to obtain a mixed solution; (2) keeping the temperature of the mixed solution to be 30-90 DEG C, adding citric acid and acetic anhydride, and stirring for 1-4 hours; (3) continuously adding superfine red clay, feldspar powder, nano-hydroxyapatite, coral reef and mannitol, and continuously stirring; (4) weighing a solution obtained in the step (3), pouring the solution into a container, putting the container into a freezing chamber with the temperature of -80 DEG C to -20 DEG C to freeze for 12-48 hours, and transferring the container into a freeze drier to perform freeze-drying for 6-36 hours, thereby obtaining the tissue-engineering three-dimensional porous bracket. The three-dimensional porous bracket provided by the invention has very good substance exchange inside and outside the bracket, and has very good biomechanical property.
Description
Technical field
The present invention relates to field of medical materials, and in particular to a kind of organizational project three-dimensional porous rack and preparation method thereof.
Background technology
Organizational project is, using zoobiotism and engineering principles and method, to go the normal and diseased tissue for recognizing mammal
Structure-function relationship, to study, be developed for repair and safeguard or promote the various tissues of human body or the function after organ injury
With a new disciplines of form.The twentieth century organizational project eighties just causes worldwide concern once proposition.Through
The research of more than ten years, organizational project is developed rapidly.Timbering material for organizational project is good except having
Outside the biocompatible properties such as biocompatibility, cellular affinity, degradability, it is necessary to meet certain mechanical property and physics
Performance, such as：Suitable aperture, porosity, higher specific surface area etc..Say from the angle of cell culture, support only in this way
Just can play a supportive role, and the transport of favourable nutriment and metabolite is exchanged, and to cell good growing environment is provided.Although porous
The exploration from the initial stage of the technology of preparing of support has many performance requirements not reach gradually forward to clinical practice stage development
Arrive, such as：High porosity and it is capable of the three dimensional structure of internal UNICOM, such structure can be exchanged and generation for cell growth, nutrient
The circulation for thanking to product provides enough spaces；There are enough mechanical performances again simultaneously, meet the stress demand in internal milieu.
The content of the invention
The technical problem to be solved：It is an object of the invention to provide a kind of organizational project three-dimensional porous rack, porosity height,
Hole connectedness is good, and mechanical property is preferable, with mass exchange and biomechanical property inside and outside good support.
Technical scheme：A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：Modified starch
10-20 parts, hyaluronate sodium 15-35 parts, bone collagen 2-15 parts, nanometer hydroxyapatite 60-75 parts, coral reef 5-15
Part, citric acid 1.5-3 parts, acetic anhydride 1.8-3.6 parts, Mannitol 4.5-16.5 part, soybean protein isolate 12-30 parts, milk surum egg
White 10-25 parts, gluten meal 5-10 parts, ultra-fine red clay 2-8 parts, feldspar powder 2-8 parts.
It is further preferred that a kind of described organizational project three-dimensional porous rack, prepared by weight by following component and
Into：Modified starch 13-18 parts, hyaluronate sodium 20-30 parts, bone collagen 5-12 parts, nanometer hydroxyapatite 64-70 parts,
Coral reef 8-12 parts, citric acid 2-2.6 parts, acetic anhydride 2.5-3 parts, Mannitol 8-14 part, soybean protein isolate 18-25 parts, breast
Albumin 15-20 parts, gluten meal 6-9 parts, ultra-fine red clay 3-7 parts, feldspar powder 4-6 parts.
The preparation method of above-mentioned organizational project three-dimensional porous rack, comprises the following steps：
（1）By modified starch, hyaluronate sodium, bone collagen, soybean protein isolate, lactalbumin, gluten meal, 2 times are added
Weight portion, and be heated in 45-90 DEG C of water, 0.5-3h is sufficiently stirred for, obtain mixed solution；
（2）It is 30-90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1-4h；
（3）Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are continuously added, continues to stir；
（4）Weigh step（3）Solution is obtained, in pouring into container, freezing 12-48 hours in -20 DEG C of refrigerating chambers of -80- is subsequently placed in
Afterwards, then lyophilization 6-36 hours in freezer dryer are moved into, obtains final product organizational project three-dimensional porous rack.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（1）Middle water temperature is
60-80 DEG C, mixing time is 1-2h.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（2）Middle temperature is
45-75 DEG C, mixing time is 3h.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（3）In it is ultra-fine glutinous
The fineness of soil and feldspar powder is 200-300 mesh.
Further, the preparation method of described a kind of organizational project three-dimensional porous rack, the step（4）Middle refrigerating chamber
Temperature be -30 DEG C of -65-, cooling time is 24-48h, the cryodesiccated time be 12-24h.
Beneficial effect：The porosity of the organizational project three-dimensional porous rack of the present invention has good between 85.5 to 92.1%
Hole connectivity；Comprcssive strength is can ensure that on the basis of high porosity is kept for 2.49-3.12MPa, it is ultra-fine adding
After red clay, feldspar powder, the mechanical property of porous support has significant raising.
Specific embodiment
Embodiment 1
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：20 parts of modified starch, hyaluronic acid
It is 35 parts of sodium, 2 parts of bone collagen, 60 parts of nanometer hydroxyapatite, 15 parts of coral reef, 1.5 parts of citric acid, 1.8 parts of acetic anhydride, sweet
4.5 parts of alcohol of dew, 12 parts of soybean protein isolate, 25 parts of lactalbumin, 5 parts of gluten meal, 2 parts of ultra-fine red clay, 2 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 45 DEG C of water, are sufficiently stirred for
3h, obtains mixed solution；（2）It is 30 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 4h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 48 hours in -20 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 6 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 2
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：10 parts of modified starch, hyaluronic acid
15 parts of sodium, 15 parts of bone collagen, 75 parts of nanometer hydroxyapatite, 5 parts of coral reef, 3 parts of citric acid, 3.6 parts of acetic anhydride, manna
16.5 parts of alcohol, 30 parts of soybean protein isolate, 10 parts of lactalbumin, 10 parts of gluten meal, 8 parts of ultra-fine red clay, 8 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 90 DEG C of water, are sufficiently stirred for
0.5h, obtains mixed solution；（2）It is 90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1h；（3）After
It is continuous to add ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）
Solution is obtained, in pouring into container, is subsequently placed in after freezing 12 hours in -80 DEG C of refrigerating chambers, then move into freezing in freezer dryer
It is dried 36 hours, obtains final product organizational project three-dimensional porous rack.
Embodiment 3
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：18 parts of modified starch, hyaluronic acid
20 parts of sodium, 5 parts of bone collagen, 64 parts of nanometer hydroxyapatite, 12 parts of coral reef, 2 parts of citric acid, 2.5 parts of acetic anhydride, manna
8 parts of alcohol, 25 parts of soybean protein isolate, 15 parts of lactalbumin, 6 parts of gluten meal, 3 parts of ultra-fine red clay, 6 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 60 DEG C of water, are sufficiently stirred for
2h, obtains mixed solution；（2）It is 45 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 24 hours in -65 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 12 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 4
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：13 parts of modified starch, hyaluronic acid
30 parts of sodium, 12 parts of bone collagen, 70 parts of nanometer hydroxyapatite, 8 parts of coral reef, 2.6 parts of citric acid, 3 parts of acetic anhydride, manna
14 parts of alcohol, 18 parts of soybean protein isolate, 20 parts of lactalbumin, 9 parts of gluten meal, 7 parts of ultra-fine red clay, 4 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 80 DEG C of water, are sufficiently stirred for
1h, obtains mixed solution；（2）It is 75 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）Continue
Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are added, continues to stir；（4）Weigh step（3）
To solution, in pouring into container, it is subsequently placed in after freezing 48 hours in -30 DEG C of refrigerating chambers, then moves into freezing in freezer dryer and does
Dry 24 hours, obtain final product organizational project three-dimensional porous rack.
Embodiment 5
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：15 parts of modified starch, hyaluronic acid
It is 25 parts of sodium, 9 parts of bone collagen, 68 parts of nanometer hydroxyapatite, 10 parts of coral reef, 2.4 parts of citric acid, 2.7 parts of acetic anhydride, sweet
12 parts of alcohol of dew, 22 parts of soybean protein isolate, 18 parts of lactalbumin, 8 parts of gluten meal, 5 parts of ultra-fine red clay, 5 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 70 DEG C of water, are sufficiently stirred for
1.5h, obtains mixed solution；（2）It is 60 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 3h；（3）After
It is continuous to add ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）
Solution is obtained, in pouring into container, is subsequently placed in after freezing 36 hours in -40 DEG C of refrigerating chambers, then move into freezing in freezer dryer
It is dried 18 hours, obtains final product organizational project three-dimensional porous rack.
Comparative example 1
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：20 parts of modified starch, hyaluronic acid
It is 35 parts of sodium, 2 parts of bone collagen, 60 parts of nanometer hydroxyapatite, 15 parts of coral reef, 1.5 parts of citric acid, 1.8 parts of acetic anhydride, sweet
4.5 parts of alcohol of dew, 2 parts of ultra-fine red clay, 2 parts of feldspar powder.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, adds 2 times of weight portions, and is heated in 45 DEG C of water, is sufficiently stirred for 3h, obtains mixed solution；（2）Mixed solution is protected
Temperature is held for 30 DEG C, citric acid, acetic anhydride is added, 4h is stirred；（3）Continuously add ultra-fine red clay, feldspar powder, nano hydroxyapatite
Lime stone, coral reef, Mannitol, continue to stir；（4）Weigh step（3）Obtain solution, in pouring into container, be subsequently placed in -20 DEG C it is cold
Freeze after freezing 48 hours in room, then move into lyophilization 6 hours in freezer dryer, obtain final product organizational project three-dimensional porous rack.
Comparative example 2
A kind of organizational project three-dimensional porous rack, is prepared from by weight by following component：10 parts of modified starch, hyaluronic acid
15 parts of sodium, 15 parts of bone collagen, 75 parts of nanometer hydroxyapatite, 5 parts of coral reef, 3 parts of citric acid, 3.6 parts of acetic anhydride, manna
16.5 parts of alcohol, 30 parts of soybean protein isolate, 10 parts of lactalbumin, 10 parts of gluten meal.
The preparation method of above-mentioned organizational project three-dimensional porous rack is：（1）By modified starch, hyaluronate sodium, ossein
Albumen, soybean protein isolate, lactalbumin, gluten meal, add 2 times of weight portions, and are heated in 90 DEG C of water, are sufficiently stirred for
0.5h, obtains mixed solution；（2）It is 90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1h；（3）After
It is continuous to add nanometer hydroxyapatite, coral reef, Mannitol, continue to stir；（4）Weigh step（3）Solution is obtained, container is poured into
In, it is subsequently placed in after freezing 12 hours in -80 DEG C of refrigerating chambers, then lyophilization 36 hours in freezer dryer are moved into, obtain final product group
Weaver's journey three-dimensional porous rack.
The method of testing of porosity：The porosity of porous support is measured with liquid substitution method.It is V first to measure its volume,
W is weighed as, ultrasound 5min in dehydrated alcohol is dipped in, is then placed under vacuum environment, negative pressure fully penetrates into dehydrated alcohol
Timbering material, taking-up is weighed as W1, and dehydrated alcohol density is designated as d, then the porosity of porous material：P=（W1-W）/（V·d）,
5 parallel tests are done, its meansigma methods is sought.
Mechanical measuring and calculation method：With Japanese Shimadzu material mechanical test machine（AGIC 50KN）Before and after determining porous material modification
Comprcssive strength, sample for 5mm × 5mm × 10mm cube, test speed is 0.5mm/min, and compression height ratio is 60%,
Every group of sample does 5 parallel laboratory tests.
The property indices of material of the present invention see the table below, it may be seen that the hole of this organizational project three-dimensional porous rack
Gap rate has good hole connectivity between 85.5 to 92.1%；Pressure resistance is can ensure that on the basis of high porosity is kept
Spend for 2.49-3.12MPa, after ultra-fine red clay, feldspar powder is added, the mechanical property of porous support has significant raising.
The property indices of the organizational project three-dimensional porous rack of table 1
| Name of product | Porosity（%） | Comprcssive strength（MPa） |
| Embodiment 1 | 85.5 | 2.49 |
| Embodiment 2 | 87.2 | 2.55 |
| Embodiment 3 | 88.5 | 2.65 |
| Embodiment 4 | 89.6 | 2.87 |
| Embodiment 5 | 92.1 | 3.12 |
| Comparative example 1 | 70.5 | 2.50 |
| Comparative example 2 | 86.1 | 1.41 |
Claims (7)
1. a kind of organizational project three-dimensional porous rack, it is characterised in that be prepared from by weight by following component：Modified starch
10-20 parts, hyaluronate sodium 15-35 parts, bone collagen 2-15 parts, nanometer hydroxyapatite 60-75 parts, coral reef 5-15
Part, citric acid 1.5-3 parts, acetic anhydride 1.8-3.6 parts, Mannitol 4.5-16.5 part, soybean protein isolate 12-30 parts, milk surum egg
White 10-25 parts, gluten meal 5-10 parts, ultra-fine red clay 2-8 parts, feldspar powder 2-8 parts.
2. a kind of organizational project three-dimensional porous rack according to claim 1, it is characterised in that by following component by weight
Part is prepared from：Modified starch 13-18 parts, hyaluronate sodium 20-30 parts, bone collagen 5-12 parts, nanometer hydroxyapatite
64-70 parts, coral reef 8-12 parts, citric acid 2-2.6 parts, acetic anhydride 2.5-3 parts, Mannitol 8-14 part, soybean protein isolate 18-
25 parts, lactalbumin 15-20 parts, gluten meal 6-9 parts, ultra-fine red clay 3-7 parts, feldspar powder 4-6 parts.
3. a kind of preparation method of organizational project three-dimensional porous rack, it is characterised in that comprise the following steps：
（1）By modified starch, hyaluronate sodium, bone collagen, soybean protein isolate, lactalbumin, gluten meal, 2 times are added
Weight portion, and be heated in 45-90 DEG C of water, 0.5-3h is sufficiently stirred for, obtain mixed solution；
（2）It is 30-90 DEG C by mixed solution keeping temperature, adds citric acid, acetic anhydride, stirs 1-4h；
（3）Ultra-fine red clay, feldspar powder, nanometer hydroxyapatite, coral reef, Mannitol are continuously added, continues to stir；
（4）Weigh step（3）Solution is obtained, in pouring into container, freezing 12-48 hours in -20 DEG C of refrigerating chambers of -80- is subsequently placed in
Afterwards, then lyophilization 6-36 hours in freezer dryer are moved into, obtains final product organizational project three-dimensional porous rack.
4. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（1）Middle water temperature is 60-80 DEG C, and mixing time is 1-2h.
5. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（2）Middle temperature is 45-75 DEG C, and mixing time is 3h.
6. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：The step
Suddenly（3）In ultra-fine clay and feldspar powder fineness be 200-300 mesh.
7. the preparation method of a kind of organizational project three-dimensional porous rack according to claim 3, it is characterised in that：It is described
Step（4）The temperature of middle refrigerating chamber is -30 DEG C of -65-, and cooling time is 24-48h, and the cryodesiccated time is 12-24h.
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