CN106608837A - Method for extracting gamma-aminobutyric acid from mulberry leaves - Google Patents
Method for extracting gamma-aminobutyric acid from mulberry leaves Download PDFInfo
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- CN106608837A CN106608837A CN201510686900.0A CN201510686900A CN106608837A CN 106608837 A CN106608837 A CN 106608837A CN 201510686900 A CN201510686900 A CN 201510686900A CN 106608837 A CN106608837 A CN 106608837A
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- Prior art keywords
- aminobutyric acid
- extract
- folium mori
- concentrated solution
- gamma
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title claims abstract description 99
- 229960003692 gamma aminobutyric acid Drugs 0.000 title claims abstract description 54
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 35
- 240000000249 Morus alba Species 0.000 title claims abstract description 21
- 235000008708 Morus alba Nutrition 0.000 title claims abstract description 21
- 239000000284 extract Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000008367 deionised water Substances 0.000 claims abstract description 7
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000000859 sublimation Methods 0.000 claims abstract description 4
- 230000008022 sublimation Effects 0.000 claims abstract description 4
- 239000000126 substance Substances 0.000 claims abstract description 4
- 239000012141 concentrate Substances 0.000 claims abstract 3
- 230000008569 process Effects 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 6
- 239000013078 crystal Substances 0.000 claims description 5
- 238000005352 clarification Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000004821 distillation Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 4
- 229910052753 mercury Inorganic materials 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 230000007480 spreading Effects 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 4
- 230000036772 blood pressure Effects 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 210000004556 brain Anatomy 0.000 abstract description 3
- 210000002569 neuron Anatomy 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 2
- 230000017531 blood circulation Effects 0.000 abstract 1
- 230000002490 cerebral effect Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000003908 liver function Effects 0.000 abstract 1
- 230000003340 mental effect Effects 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 238000005057 refrigeration Methods 0.000 abstract 1
- 238000000638 solvent extraction Methods 0.000 abstract 1
- 229920003266 Leaf® Polymers 0.000 description 16
- 235000013305 food Nutrition 0.000 description 4
- 239000002994 raw material Substances 0.000 description 3
- 230000008344 brain blood flow Effects 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000021329 brown rice Nutrition 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for extracting gamma-aminobutyric acid from mulberry leaves. The method comprises the following five process steps: withering; anaerobic treatment; extraction; concentration; and drying. The method is characterized in that a part of moisture in fresh mulberry leaves is removed through withering; repeated anaerobic treatment is carried out for enrichment gamma-aminobutyric acid; deionized water is used for solvent extraction of soluble substances in the mulberry leaves so as to obtain extract; the extract is concentrated so as to obtain a concentrate; and the concentrate is subjected to refrigeration, sublimation and drying so as to obtain gamma-aminobutyric acid. The method provided by the invention has the characteristics of simple process steps and high extraction rate and high purity of gamma-aminobutyric acid. The gamma-aminobutyric acid obtained in the invention is capable of adjusting blood pressure and heart rate, nourishing nerve cells, promoting mental tranquilization and blood circulation in the brain, improving cerebral vitality, lowering blood pressure, enhancing liver functions, promoting alcohol metabolism, eliminating odor, etc.
Description
Technical field
The invention belongs to food processing technology field, and in particular to one kind extracts γ-aminobutyric acid preparation method from Folium Mori.
Background technology
γ-aminobutyric acid is a kind of feature acidic amino acid of nonprotein, it is widely present in nature, it is the important inhibitory neurotransmitter in mammalian central nervous system, with important body function, there are regulation blood pressure and heart rate, trophic nerve cell, promote ataraxy, promote brain blood flow, promote the multiple efficacies such as brain vigor, GABA content in natural food materials is mainly improved by concentration method, add Glu or using the Glu contained in itself in food materials using external source, the GAD enzymes that activation contains in itself, it is enriched with, current main brown rice, Folium Mori etc. are enriched with, because Glu is limited in raw material, the simple Glu using raw material itself, there is concentration of substrate to be limited, GAD enzymes are using incomplete, the problems such as product GABA content is not high.External source addition Glu can increase substantially product G ABA content.
With the further investigation of GABA, GABA is widely used in the industries such as medicine, food, health care, chemical industry and agricultural by as a kind of new function sex factor.China's Morus alba Resource Development is enriched, and research finds the GABA containing high-load in Folium Mori, and GABA average contents are 226mg in 100g Folium Mori dry products, so γ-aminobutyric acid is extracted from Folium Mori being increasingly subject to people's attention, wide market.The Chinese patent literature of Application No. 200910040513.4 discloses mulberry leaf gamma-aminobutyric acid extracting method and its utilization；With fresh mulberry leaf as raw material, drying is crushed, and is soaked, ultrasonic extraction, and dehydrated alcohol precipitation, three-level the operation such as isolates and purifies and γ-aminobutyric acid white crystal is obtained；But the method has production cost height, the utilization rate of Folium Mori is low.
The content of the invention
The invention provides it is a kind of from Folium Mori extract γ-aminobutyric acid method, overcome above-mentioned prior art no
Foot, its existing γ-aminobutyric acid extracting method of energy effectively solving has that high and Folium Mori the utilization rate of production cost is low.
To achieve these goals, the present invention proposes following technical scheme：
One kind extracts γ-aminobutyric acid preparation method from Folium Mori, and the method is comprised the following steps：
(1) wither：It is 50-70% that fresh mulberry leaf withers to water content；
(2) anaerobism anaerobic treatment：Fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h,
So processing 2-3 time repeatedly carries out γ-aminobutyric acid enrichment；
(3) extract：By the fresh mulberry leaf after enrichment, deionized water is added, blended, extract 15-30min, clarify 30-50min, be centrifuged under the conditions of 7000rpm, obtain clarification Mori folium extract；
（4）Concentration：Extracting solution is concentrated, concentrated solution is obtained；
（5）By concentrated solution drying bu sublimation, γ-aminobutyric acid is obtained.
Preferably, the step（3）Described in add deionized water and the fresh mulberry leaf mass volume ratio be 1:1.
Preferably, the step (3) is that Folium Mori are placed in into the soluble substance that 15-30min in deionized water extracts Folium Mori, obtains extracting solution.
Preferably, the step (4) is less than 55 DEG C by extracting solution more than 0.1MPa vacuums, is concentrated into 15-20 Baume degrees, obtains first time concentrated solution, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.
Preferably, it by second concentrated solution in step (4) in temperature is -30 DEG C that the step (5) is, vacuum is to be freezed under the conditions of 0.1～0.06 millimetres of mercury, then carries out ice crystal distillation, finally obtains γ-aminobutyric acid.
A kind of method that γ-aminobutyric acid is extracted from Folium Mori provided by the present invention, make in Folium Mori rich in GABA, become a kind of with the γ-aminobutyric acid for adjusting blood pressure and heart rate, trophic nerve cell, promote ataraxy, promote brain blood flow, promote the multiple efficacies such as brain vigor.
Compared with prior art, it is also manifested by using beneficial effects of the present invention：
First, processing step is simple, and Folium Mori are through step（3）The extracting solution obtained after extraction, extraction ratio is high；
2nd, extracting solution is through step（4）After concentrating twice, the concentrated solution liquid for obtaining is limpid bright, without precipitation and research of chaotic phenomenon；
3rd, second concentrated solution is through step（5）After drying bu sublimation, the γ-aminobutyric acid for obtaining has the characteristics of solubility is good, purity is high.
The additional aspect and advantage of the present invention will be set forth in part in the description, and partly will become apparent from the description below, or be recognized by the practice of the present invention.
Specific embodiment
Technical scheme is described further below by embodiment, but is not considered as limiting the invention.
Embodiment 1
It is 50-70% to take 5000 grams of fresh mulberry leaf and wither to water content, fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, so processing 2 times repeatedly carries out γ-aminobutyric acid enrichment, and GABA content reaches 2.52mg/g in dry Folium Mori after enrichment；Fresh mulberry leaf after being enriched with, adds 5000ml deionized waters, blends, stirring and leaching 20min, stands clarification 30min, is centrifuged under the conditions of 7000rpm, must clarify extracting solution 4000ml.By extracting solution more than 0.1MPa vacuums, temperature obtains first time concentrated solution under the conditions of 50 DEG C, to be concentrated into 18 Baume degrees, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.In temperature it is -30 DEG C by second concentrated solution, vacuum is to be freezed under the conditions of 0.09 millimetres of mercury, then carries out ice crystal distillation, and the γ-aminobutyric acid of 2.95g or so is finally obtained, and 100g fresh mulberry leafs extract 59.00mg γ-aminobutyric acid.
γ-aminobutyric acid that the present invention is obtained is faint yellow or the purity of white powder γ-aminobutyric acid after testing averagely reaches 90%, and the existing mulberry leaf gamma-aminobutyric acid extracting method and its content of γ-aminobutyric acid is suitable in γ-aminobutyric acid white crystalline body in using.
Embodiment 2
It is 60-70% to take fresh mulberry leaf 40Kg and wither to water content, and the fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, and so processing 2 times repeatedly carries out γ-aminobutyric acid enrichment, and GABA content reaches 2.48mg/g in dry Folium Mori after enrichment；Fresh mulberry leaf after being enriched with, adds 40L deionized waters, blends, stirring and leaching 30min, stands clarification 30min, is centrifuged under the conditions of 7000rpm, must clarify extracting solution 33L.By extracting solution more than 0.1MPa vacuums, temperature obtains first time concentrated solution under the conditions of 45 DEG C, to be concentrated into 15 Baume degrees, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.In temperature it is -30 DEG C by second concentrated solution, vacuum is to be freezed under the conditions of 0.09 millimetres of mercury, then carries out ice crystal distillation, and the γ-aminobutyric acid of 23.12g or so is finally obtained, and 100g fresh mulberry leafs extract 57.80mg γ-aminobutyric acid.
γ-aminobutyric acid that the present invention is obtained is faint yellow or the purity of white powder γ-aminobutyric acid after testing averagely reaches 90%, and the existing mulberry leaf gamma-aminobutyric acid extracting method and its content of γ-aminobutyric acid is suitable in γ-aminobutyric acid white crystalline body in using.
The invention is not restricted to above example, but all should fall within the claims in the present invention protection domain.
Claims (5)
1. it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterised in that the method is comprised the following steps：
(1) wither：It is 50-70% that fresh mulberry leaf withers to water content；
(2) anaerobism anaerobic treatment：Fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, and so processing 2-3 time repeatedly carries out γ-aminobutyric acid enrichment；
(3) extract：By the fresh mulberry leaf after enrichment, deionized water is added, blended, extract 15-30min, clarify 30-50min, be centrifuged under the conditions of 7000rpm, obtain clarification Mori folium extract；
(4) concentrate：Extracting solution is concentrated, concentrated solution is obtained；
(5) it is dried：By concentrated solution drying bu sublimation, γ-aminobutyric acid is obtained.
2. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterised in that the step（3）Described in add deionized water and the fresh mulberry leaf mass volume ratio be 1:1.
3. a kind of method that γ-aminobutyric acid is extracted from Folium Mori according to claim 1, it is characterised in that the step (3) is that Folium Mori are placed in into the soluble substance that 15-30min in deionized water extracts Folium Mori, obtains extracting solution.
4. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterized in that, the step (4) be by extracting solution more than 0.1MPa vacuums, less than 55 DEG C, it is concentrated into 15-20 Baume degrees, first time concentrated solution is obtained, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.
5. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterized in that, the step (5) be by second concentrated solution in step (4) temperature be -30 DEG C, vacuum is to be freezed under the conditions of 0.1～0.06 millimetres of mercury, ice crystal distillation is carried out again, finally obtains γ-aminobutyric acid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510686900.0A CN106608837A (en) | 2015-10-22 | 2015-10-22 | Method for extracting gamma-aminobutyric acid from mulberry leaves |
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| CN201510686900.0A CN106608837A (en) | 2015-10-22 | 2015-10-22 | Method for extracting gamma-aminobutyric acid from mulberry leaves |
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| CN106608837A true CN106608837A (en) | 2017-05-03 |
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| CN201510686900.0A Pending CN106608837A (en) | 2015-10-22 | 2015-10-22 | Method for extracting gamma-aminobutyric acid from mulberry leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115477592A (en) * | 2022-09-30 | 2022-12-16 | 西安萃源生物科技有限公司 | Crystallization method of gamma-aminobutyric acid |
| CN118255680A (en) * | 2024-03-27 | 2024-06-28 | 金木集团有限公司 | A method for extracting purified gamma-aminobutyric acid from ice plant |
-
2015
- 2015-10-22 CN CN201510686900.0A patent/CN106608837A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115477592A (en) * | 2022-09-30 | 2022-12-16 | 西安萃源生物科技有限公司 | Crystallization method of gamma-aminobutyric acid |
| CN118255680A (en) * | 2024-03-27 | 2024-06-28 | 金木集团有限公司 | A method for extracting purified gamma-aminobutyric acid from ice plant |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170503 |
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| WD01 | Invention patent application deemed withdrawn after publication |