CN106578961A - Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method - Google Patents
Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method Download PDFInfo
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- CN106578961A CN106578961A CN201611146609.5A CN201611146609A CN106578961A CN 106578961 A CN106578961 A CN 106578961A CN 201611146609 A CN201611146609 A CN 201611146609A CN 106578961 A CN106578961 A CN 106578961A
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- Prior art keywords
- semen
- fermentation
- peptide
- oral liquid
- semen phaseoli
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 46
- 238000000855 fermentation Methods 0.000 title claims abstract description 40
- 230000004151 fermentation Effects 0.000 title claims abstract description 40
- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 230000000694 effects Effects 0.000 title claims abstract description 18
- 240000001417 Vigna umbellata Species 0.000 title abstract 5
- 235000011453 Vigna umbellata Nutrition 0.000 title abstract 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title abstract 2
- 239000008280 blood Substances 0.000 title abstract 2
- 210000004369 blood Anatomy 0.000 title abstract 2
- 230000036772 blood pressure Effects 0.000 title abstract 2
- 239000008103 glucose Substances 0.000 title abstract 2
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 36
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 36
- 210000000582 semen Anatomy 0.000 claims description 88
- 239000012153 distilled water Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 17
- 239000007921 spray Substances 0.000 claims description 15
- 230000004913 activation Effects 0.000 claims description 13
- 238000010025 steaming Methods 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 5
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 5
- 230000004907 flux Effects 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 230000035479 physiological effects, processes and functions Effects 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000000796 flavoring agent Substances 0.000 abstract description 5
- 235000019634 flavors Nutrition 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 3
- 230000000813 microbial effect Effects 0.000 abstract 3
- 239000000243 solution Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000001976 enzyme digestion Methods 0.000 description 6
- 239000002504 physiological saline solution Substances 0.000 description 4
- 235000019658 bitter taste Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using a fermentation method. Small red beans are fermented by aspergillus oryzae, functional peptide and DPP-4 are obtained by a microbial fermentation means, and the red bean peptide oral liquid is prepared. The method has the advantages that in the preparation process of the red bean peptide, the flavor of substances generated by microbial fermentation is better than the flavor of substances generated by enzymatic hydrolysis, the safety is high, the peptide yield is high, and the shortcoming that functional substances are bitter in a process of enzymatic hydrolysis is further overcome; and compared with enzymatic hydrolysis, microbial fermentation is shorter in period, easier to control and lower in cost.
Description
Technical field
The present invention relates to a kind of Semen Phaseoli peptide oral liquid manufacture method, and in particular to a kind of fermentation method makes has reducing pressure and sugar
The Semen Phaseoli peptide oral liquid method of effect.
Background technology
At present preparations of country's Semen Phaseoli peptide be by way of enzymolysis obtaining, but using enzymatic isolation method with as follows
Shortcoming:
1. during adopting the Semen Phaseoli peptide of enzyme hydrolysiss legal system, bitter substance saponin can be supervened.
2. enzymolysis process is wayward.
3. the Semen Phaseoli peptide yield by enzyme digestion reaction generation is relatively low.
The content of the invention
The purpose of the present invention is for deficiency of the prior art, there is provided a kind of fermentation method makes has reducing pressure and sugar effect
Semen Phaseoli peptide oral liquid method, the cycle is shorter, is more easy to manipulation, and cost is lower.
For achieving the above object, the invention discloses following technical scheme:
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little
Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
Further, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermenting.
Further, comprising the following steps that for Semen Ormosiae Hosiei mud is made:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100-150 DEG C of autoclave, 20-40min sterilizings is placed on.
Further, aspergillus oryzae is carried out into process activation before fermenting.
Further, comprising the following steps that for aspergillus oryzae is activated:Aspergillus oryzae is activated under conditions of 30-35 DEG C 40-
50h。
Further, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 20-35 DEG C
Fermentation 72h, takes 50-80 parts fermented product and mixes with 120-180 part distilled water and stir evenly, and 80-100 DEG C is heated 20-45min, cooled
Filtering must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
Further, comprise the following steps that:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, pH value is adjusted to 6.0,121 DEG C of autoclave, 30min is placed on
Sterilizing, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, aspergillus oryzae strain is taken into 3 inoculating loops and is inoculated in culture medium,
31 DEG C, it is inoculated in triangular flask after culture 48h and is enlarged culture, then uses the aspergillus oryzae for having activated in the case of without miscellaneous bacteria
Physiological saline solution washes lower spore, and concentration is adjusted to into 108Individual/mL, sprays on Semen Ormosiae Hosiei mud, and spray flux is every 1000g
Semen Ormosiae Hosiei mud sprays 3mL bacterium solutions, 30 DEG C of Jing fermentation 72h, takes 75 parts of fermented products and mixes with 150 parts of distilled water and stirs evenly, and 90 DEG C are heated
30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
A kind of fermentation method disclosed by the invention makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, with following
Beneficial effect:
1. in the preparation process of Semen Phaseoli peptide, fermentable is more preferable than the material local flavor that enzyme digestion reaction is produced, safety
Higher, peptide yield is higher, while also solving the shortcoming of functional mass during enzyme digestion reaction with bitterness.
2. fermentable is compared with enzyme digestion reaction, and the cycle is shorter, is more easy to manipulation, and cost is lower.
Description of the drawings
Fig. 1 is the process chart of the present invention.
Specific embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the invention, rather than the embodiment of whole.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model of present invention protection
Enclose.
The core of the present invention is to provide a kind of fermentation method and makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
Safe, peptide yield is high, the functional oral liquid feature containing Semen Ormosiae Hosiei peptide is made with it and local flavor is more preferable.
Refer to Fig. 1.
Embodiment 1
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little
Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100 DEG C of autoclave, 20min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will
Aspergillus oryzae activates 40h under conditions of 30 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud,
20 DEG C of fermentation 72h, take 50 parts of fermented products and mix with 120 parts of distilled water and stir evenly, and 80 DEG C are heated 20min, and cold filtration must contain Semen Phaseoli
The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted
Be worth to 6.0, be placed on 100 DEG C of autoclave, 20min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae
Strain takes 3 inoculating loops and is inoculated in culture medium, 30 DEG C, is inoculated in triangular flask after culture 40h and is enlarged culture, then will activation
Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray
Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 20 DEG C of fermentation 72h of Jing take 50 parts of fermented products
Mix with 120 parts of distilled water and stir evenly, 80 DEG C of heating 20min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming
Distilled water is measured using parts by weight.
Embodiment 2
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little
Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 150 DEG C of autoclave, 40min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will
Aspergillus oryzae activates 50h under conditions of 35 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud,
35 DEG C of fermentation 72h, take 80 parts of fermented products and mix with 180 parts of distilled water and stir evenly, and 100 DEG C are heated 45min, and cold filtration must contain Semen Phaseoli
The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted
Be worth to 6.0, be placed on 150 DEG C of autoclave, 40min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae
Strain takes 3 inoculating loops and is inoculated in culture medium, 35 DEG C, is inoculated in triangular flask after culture 50h and is enlarged culture, then will activation
Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray
Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 30 DEG C of fermentation 72h of Jing take 80 parts of fermented products
Mix with 180 parts of distilled water and stir evenly, 100 DEG C of heating 45min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming
Distilled water is measured using parts by weight.
Embodiment 3
A kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, is fermented by aspergillus oryzae red little
Bean, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
As specific embodiment, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation, make comprising the following steps that for Semen Ormosiae Hosiei mud:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 121 DEG C of autoclave, 30min sterilizings is placed on.
As specific embodiment, aspergillus oryzae is carried out into process activation before fermentation, activate comprising the following steps that for aspergillus oryzae:Will
Aspergillus oryzae activates 48h under conditions of 31 DEG C.
As specific embodiment, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud,
30 DEG C of fermentation 72h, take 75 parts of fermented products and mix with 150 parts of distilled water and stir evenly, and 90 DEG C are heated 30min, and cold filtration must contain Semen Phaseoli
The solution of peptide, wherein, fermented product and distilled water are measured using parts by weight.
As specific embodiment, comprise the following steps that:Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, PH is adjusted
Be worth to 6.0, be placed on 121 DEG C of autoclave, 30min sterilizings, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, by aspergillus oryzae
Strain takes 3 inoculating loops and is inoculated in culture medium, 31 DEG C, is inoculated in triangular flask after culture 48h and is enlarged culture, then will activation
Good aspergillus oryzae washes lower spore in the case of without miscellaneous bacteria with physiological saline solution, and its concentration is adjusted to into 108Individual/mL, spray
Drench on Semen Ormosiae Hosiei mud, spray flux is every 1000g Semen Ormosiae Hosiei mud spray 3mL bacterium solutions, 30 DEG C of fermentation 72h of Jing take 75 parts of fermented products
Mix with 150 parts of distilled water and stir evenly, 90 DEG C of heating 30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and steaming
Distilled water is measured using parts by weight.
The content introduced in background technology is compared, more preferably, safety is higher, peptide for Semen Phaseoli peptide material local flavor prepared by the present invention
Yield is higher, while the shortcoming of functional mass during enzyme digestion reaction with bitterness is also solved, and compared with enzyme digestion reaction, this
The invention cycle is shorter, is more easy to manipulation, and cost is lower.
The above is only the preferred embodiment of the present invention, rather than a limitation;Although it should be pointed out that with reference to above-mentioned each
Embodiment has been described in detail to the present invention, it will be understood by those within the art that, it still can be to above-mentioned each
Technical scheme described in embodiment is modified, or carries out equivalent to which part or all technical characteristic;And this
A little modifications and replacement, do not make the scope of the essence disengaging various embodiments of the present invention technical scheme of corresponding technical scheme.
Claims (7)
1. a kind of fermentation method makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, it is characterised in that by aspergillus oryzae
Fermentation Semen Ormosiae Hosiei, using the means of fermentable functional peptide and DPP-4 are obtained, and Semen Phaseoli peptide oral liquid is obtained.
2. a kind of fermentation method according to claim 1 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, Semen Ormosiae Hosiei is made into Semen Ormosiae Hosiei mud before fermentation.
3. a kind of fermentation method according to claim 2 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, makes comprising the following steps that for Semen Ormosiae Hosiei mud:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, 100-150 DEG C of autoclave, 20-40min sterilizings is placed on.
4. a kind of fermentation method according to claim 3 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, aspergillus oryzae is carried out into process activation before fermentation.
5. a kind of fermentation method according to claim 4 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, activates comprising the following steps that for aspergillus oryzae:Aspergillus oryzae is activated under conditions of 30-35 DEG C 40-50h.
6. a kind of fermentation method according to claim 5 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, the aspergillus oryzae after activation is diluted in the case of without miscellaneous bacteria and is sprayed on Semen Ormosiae Hosiei mud, 20-35 DEG C of fermentation 72h,
Take 50-80 parts fermented product and mix with 120-180 part distilled water and stir evenly, 80-100 DEG C of heating 20-45min, cold filtration must contain red
The solution of bean peptide, wherein, fermented product and distilled water are measured using parts by weight.
7. a kind of fermentation method according to claim 6 makes the Semen Phaseoli peptide oral liquid method with reducing pressure and sugar effect, its
It is characterised by, comprises the following steps that:
Semen Ormosiae Hosiei is cleaned into steaming and decocting after remove impurity, Semen Phaseoli paste is made, pH value is adjusted to 6.0,121 DEG C of autoclave is placed on, 30min goes out
Bacterium, water-bath be cooled to 30 DEG C it is standby, using PDA slant culture, aspergillus oryzae strain is taken into 3 inoculating loops and is inoculated in culture medium, 31
DEG C, it is inoculated in triangular flask after culture 48h and is enlarged culture, then the aspergillus oryzae for having activated is used into nothing in the case of without miscellaneous bacteria
The lower spore of bacterium physiology salt washing, and its concentration is adjusted to into 108Individual/mL, sprays on Semen Ormosiae Hosiei mud, and spray flux is every 1000g
Semen Ormosiae Hosiei mud sprays 3mL bacterium solutions, 30 DEG C of Jing fermentation 72h, takes 75 parts of fermented products and mixes with 150 parts of distilled water and stirs evenly, and 90 DEG C are heated
30min, cold filtration must contain the solution of Semen Phaseoli peptide, wherein, fermented product and distilled water are measured using parts by weight.
Priority Applications (1)
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CN201611146609.5A CN106578961A (en) | 2016-12-13 | 2016-12-13 | Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method |
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CN201611146609.5A CN106578961A (en) | 2016-12-13 | 2016-12-13 | Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method |
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CN201611146609.5A Pending CN106578961A (en) | 2016-12-13 | 2016-12-13 | Method for preparing red bean peptide oral liquid with effect of reducing blood pressure and blood glucose by using fermentation method |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111067108A (en) * | 2019-12-31 | 2020-04-28 | 北京农学院 | A kind of rose tartary buckwheat polypeptide oral liquid and preparation method thereof |
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CN1786182A (en) * | 2005-11-09 | 2006-06-14 | 天津科技大学 | Method of preparing product containing sogabean peptide by microorganism fermentation process |
CN101250575A (en) * | 2008-03-07 | 2008-08-27 | 秋生堂生物科技(北京)有限公司 | Method for preparing vanadium-containing peptides and products produced thereby |
CN102470166A (en) * | 2009-08-03 | 2012-05-23 | 株式会社钟化 | Dipeptidyl peptidase-4 inhibitor |
CN104293869A (en) * | 2014-09-10 | 2015-01-21 | 内蒙古天奇生物科技有限公司 | Bitter-free red bean polypeptide and preparation method thereof |
-
2016
- 2016-12-13 CN CN201611146609.5A patent/CN106578961A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1786182A (en) * | 2005-11-09 | 2006-06-14 | 天津科技大学 | Method of preparing product containing sogabean peptide by microorganism fermentation process |
CN101250575A (en) * | 2008-03-07 | 2008-08-27 | 秋生堂生物科技(北京)有限公司 | Method for preparing vanadium-containing peptides and products produced thereby |
CN102470166A (en) * | 2009-08-03 | 2012-05-23 | 株式会社钟化 | Dipeptidyl peptidase-4 inhibitor |
CN104293869A (en) * | 2014-09-10 | 2015-01-21 | 内蒙古天奇生物科技有限公司 | Bitter-free red bean polypeptide and preparation method thereof |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111067108A (en) * | 2019-12-31 | 2020-04-28 | 北京农学院 | A kind of rose tartary buckwheat polypeptide oral liquid and preparation method thereof |
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