CN106577593A - High purity and high survival rate live ant acquisition method - Google Patents

Abstract

The invention belongs to the field of biology, and especially relates to a biological acquisition method. The method comprises: firstly, using the habit that live ants fear water, slowing filling water into a container which holds an ant nest, to separate the live ants from the ant nest, to obtain high purity live ants, and then using the characteristic that diethyl ether and other volatile general anesthesia liquid drugs can dizzy creatures by low dosage, and putting the live ant individuals into the clean container under the condition of swoon. The method can obtain high purity and high survival rate live ants, and is suitable for acquisition of red imported fire ants, white ants, and polyrhachis dives, and other medical ants.

Description

A live ant collection method with high purity and high survival rate

technical field

The invention belongs to the field of biology, in particular to a biological collection method.

Background technique

Red fire ants and termites are two kinds of organisms that are seriously harmful to humans. Both of these creatures live in underground ant nests, each containing hundreds of thousands to millions of individuals, and are social insects.

When conducting an ant-killing efficacy test on red imported fire ants or termites in the laboratory, experimenters usually use a brush to gently pick live red imported fire ants or termites from the ant nest one by one, and put them into wide-mouthed glass bottles. Drugs were administered for observation. However, because live red fire ants or termites can crawl freely, it takes time and effort to pick them into the jar, and the insect body is soft, so it is easy to crush the insect body to death if you are not careful, or when you pick it into the jar, the insect body will be damaged. It is still alive, but it has been stabbed by a brush, and it will die soon, which will affect the accuracy of the drug efficacy test. In addition, when picking fire ants or termites directly from the ant nest, they will often be picked into the jar together with the soil particles, and the insects Body purity is low. Moreover, RIFAs also have the habit of actively attacking humans, and experimenters are often bitten by RIFAs when operating. Therefore, this experimental method has great defects.

The black thorn ant ( Polyrhachis vicina Roger) and the double-toothed thorn ant ( Polyrhachis dives Smith) are two main types of medicinal ants.

In nature, the ants mainly live in forests with sufficient water sources and high tree density and miscellaneous forests by rivers, pine forests, bamboo forests, camellia oleifera forests, and fruit forests. They are social insects that live in colonies and often build nests in trees. Up or underground, camp social life. Pseudomonas spp. (black ants) has medicinal value of nourishing kidney, strengthening bones, anti-aging, and preventing diseases, and can be used as a raw material for medicines or health products. According to the 1996 edition of Yunnan Provincial Standards for Medicinal Materials: This product is the dried body of the Formicidae insect, Pseudomonas spp. It can be harvested in four seasons, and the ant nests are put into a sealed bag to suffocate, or put into boiling water to scald to death; take out, remove impurities such as ant nests, leaves and soil, and dry the ant bodies in the sun or below 60°C.

The spiny ant is an insect belonging to the family Formicaceae. Prickly-toothed ants mostly build nests in trees, and a few build nests in grass and under stones. Ant nests can be transferred from trees to the ground in winter. The ant nest is composed of tree branches and leaves, weed debris, spinning matter, etc. The nest is honeycomb-shaped with many small chambers. The spiny ant has anti-inflammatory, analgesic and sedative, anti-asthma and antispasmodic properties, and liver-protecting medicinal value, and can be used as a raw material for medicine or health care products. According to the Hunan Provincial Standards of Chinese Medicinal Materials (2009 edition), this product is the dried adult body of the Formicaceae insect, the spiny ants. Catch them from November to March of the next year, suffocate them in a suitable container, take them out and dry them, or scald them quickly with 60°C water and dry them in the air.

The collection methods of the above two kinds of medicinal ants are time-consuming and laborious, and the surface of the insect body will adhere to soil or microorganisms, and the purity is low.

Contents of the invention

In view of this, the technical problem to be solved by the present invention is to provide a method for collecting live ants with high purity and high survival rate.

The technical problem to be solved by the present invention is realized through the following technical solutions:

A live ant collection method with high purity and high survival rate is:

(1) Apply a layer of talcum powder on the edge of the container, dig out the entire ant nest with a shovel and put it in the container;

(2) Put multiple small wooden branches on the top of the ant nest;

(3) Slowly add water to the container, so that the water is added to 1/10~2/10 from the top of the ant nest, and the speed of adding water should be slow so that the living ants can evacuate upward in time;

(4) After the live ants gather on the wooden branches at the top of the ant nest, use a long branch to gently put the wooden branches full of live ants into another clean container prepared in advance, cover it well, and put it on protective bag;

(5) Bring the container to the operating room, quickly open the lid, and drop anesthetic doses of volatile general anesthetic liquid drugs into the container;

(6) After the live ants are stunned, immediately shake off the live ants from the wooden branch, take out the wooden branch, transfer it into another container with a medicine spoon, seal it, and set aside.

Further, the living ants are small reptiles that are afraid of water and live in nests.

Furthermore, the small water-afraid reptiles living socially in the nesting camp group include red fire ants, termites or medicinal ants.

Furthermore, the medicinal ants include Pseudomonas spp. or Psychia bidenta.

Further, the volatile general anesthesia liquid drug in step (5) includes at least one of ether, enflurane, isoflurane, desflurane, and sevoflurane.

Further, the volatile general anesthesia liquid drug in step (5) is ether, and the dosage in a 2L container is 2-4 drops.

The present invention has following beneficial effects:

1. The present invention first utilizes the habit of red fire ants or termites fearing water, and adopts the method of slowly injecting water into a container equipped with ant nests to separate live ants from the ant nests to obtain high-purity live ants, and then use them to Volatile general anesthetics such as ether have the habit of stunning organisms in low doses, so that live ant individuals are put into test containers or other containers while being stunned. The method into jars saves a lot of time and improves the survival rate significantly.

2. The living ants with high purity obtained in the present invention can reduce the soil brought by harvesting ants by traditional methods, and improve the quality of raw materials Cleanliness, reducing the content of impurities, especially heavy metals, in the production of medicines or health care products using it as raw materials, and avoiding microbial contamination caused by soil and dead ants, thereby improving product quality. Moreover, the survival rate of the obtained live ants reaches 100%, and the volatile general anesthesia liquid medicines such as ether are easy to volatilize and have no residue, which ensures that people or animals are edible or medicinally safe.

detailed description

The present invention will be described in detail below in conjunction with examples, which are only preferred implementations of the present invention, and are not limitations of the present invention.

Example 1

For an RIFA drug efficacy test, individual RIFAs need to be placed in jars and administered with drugs. The test preparations are as follows:

(1) Apply a layer of talcum powder on the edge of the plastic bucket, dig out the whole ant nest with a shovel and put it in the plastic bucket;

(2) Put multiple small wooden branches on the top of the ant nest;

(3) Slowly add water to the plastic bucket so that the water is 1/10 from the top of the ant nest, and the speed of adding water should be slow so that the live ants can evacuate upwards in time, and wait for about 1 hour;

(4) After the live ants gather on the wooden branches at the top of the ant nest, gently put the wooden branches full of live ants into the clean 2L milk powder tank prepared in advance with a long branch, cover it well, and put it in a upper protective bag;

(5) Take the milk powder can to the laboratory, quickly open the lid, and drop 2 drops of ether into the container;

(6) When the living ants are stunned, immediately shake them off the wooden branch, take out the wooden branch, transfer it into the corresponding jar with a medicine spoon, seal it, and set it aside. After waiting for a while, the living ants transferred into the jar can all be revived, and the survival rate is 100%.

This method first utilizes the habit of water-fearing RIFA, and slowly injects water into the container containing the ant nest to separate the living ants from the ant nest to obtain high-purity RIFA individuals. The dosage can stun the habits of organisms, so that the live ants are put into the test jar while being stunned. This process saves a lot of time compared with the existing method of using a brush to pick live ants into the jar , and significantly increased the survival rate.

Example 2

To collect wild Pseudomonas spiny ants, the method is as follows:

(1) Apply a layer of talcum powder on the edge of the plastic bucket, dig out the whole ant nest with a shovel and put it in the container;

(2) Put multiple small wooden branches on the top of the ant nest;

(3) Slowly add water to the plastic bucket, so that the water is added to 1/10~2/10 from the top of the ant nest, and the speed of adding water should be slow so that the live ants can evacuate upward in time;

(4) After the live ants gather on the wooden branches at the top of the ant nest, use a long branch to gently put the wooden branches full of live ants into the clean 2L milk powder tank prepared in advance, cover it well, and put it on protective bag;

(5) Take the milk powder tank to the operating room, quickly open the lid, and drop 3 drops of ether into the container;

(6) When the black spiny ants are stunned, immediately shake it off the wooden branch, take out the wooden branch, transfer it into another container with a medicine spoon, seal it, and set it aside.

Wait for a while, the wild Pseudomonas ant will recover, the survival rate is 100%, and then use the wild Pseudomonas ant as medicine or health care products.

This method is also applicable to the double-toothed spiny ants.

This method has high collection efficiency, high purity, and high survival rate, which can reduce the soil brought by the traditional method of harvesting wild Pseudomonas spp. Impurities in health care products, especially the content of heavy metals, can avoid microbial contamination caused by soil and dead ants, thereby improving product quality. Moreover, the survival rate of the obtained live ants reaches 100%, and the volatile general anesthesia liquid medicines such as ether are easy to volatilize and have no residue, which ensures that people or animals are edible or medicinally safe.

Claims (6)

1. the ant acquisition method living of a kind of high cleanliness and high-survival rate, it is characterised in that：

（1）Last layer Pulvis Talci is applied at the edge of container, is taken whole ant nest with spade and is put in container；

（2）The little wooden branch of multistage on the tip shelf of ant nest；

（3）Slowly add water toward container, water is added at ant nest top 1/10 ~ 2/10, the speed for adding water is slow, so as to the ant that lives Can up withdraw in time；

（4）After the ant that lives is gathered in the wooden branch on the top of ant nest, gently the wooden branch for gathering ant living are put in advance with long branch In ready another clean container, cover, and put protective bag；

（5）By container band to operating room, lid is opened rapidly, the volatility general anesthesia liquid of Deca anaesthesia dosage into container Medicine；

（6）Ant to be lived at once is shaken off ant living from wooden branch by daze, takes out wooden branch, is proceeded in another container with spoon, Sealing, it is standby.

2. according to the ant acquisition method living described in claim 1, it is characterised in that：The ant living is the social sexual life of the battalion lived on trees Tiny reptile afraid of water.

3. ant acquisition method living according to claim 2, it is characterised in that：It is thin that the battalion's mass society lived on trees is lived Little reptile afraid of water includes red fire ant or Coptotermes formosanus Shtrari. or medical ant.

4. ant acquisition method living according to claim 3, it is characterised in that：The medical ant include Polyhachis vicina Roger or Bitooth multi-ant.

5. ant acquisition method living according to claim 1, it is characterised in that：Step（5）The volatility general anesthesia liquid Body medicine includes at least one in ether, enflurane, isoflurane, desflurane, Sevoflurane.

6. ant acquisition method living according to claim 1, it is characterised in that：Step（5）The volatility general anesthesia liquid Body medicine is ether, and 2L containers consumption is 2 ~ 4 drops.