CN106556526B - Tumor tissue three-dimensional space sampler and sampling method thereof - Google Patents
Tumor tissue three-dimensional space sampler and sampling method thereof Download PDFInfo
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Abstract
The invention relates to a tumor tissue three-dimensional space sampler and a sampling method thereof, which relate to the technical field of tumor sampling and mainly aim at improving the space sampling efficiency. The sampling method comprises the following steps: sampling: aligning a plurality of sampling needle tubes loaded on a sampling platform with tissue samples, inserting the sampling needle tubes into tumor tissue samples, and filling partial tissue samples into sampling channels of the sampling needle tubes; extracting the sampling needle tube from the tumor tissue sample; sample separation: the method comprises the steps of aligning a broken device with a sectional ring mark of a sampling needle tube for cutting, segmenting the sampling needle tube, segmenting and sampling different sampling needle tubes, and numbering and dividing samples according to the space position of the sampling needle tube where the segmented sampling needle tube is located so as to completely correspond to the space position inside a tumor. Compared with the existing manual single-point sampling technology, the method has the advantages that the time consumption is shorter, a plurality of samples on the tumor three-dimensional space can be taken at one time, and convenience is brought to the research of tumor space heterogeneity.
Description
Technical Field
The invention relates to the technical field of tumor sampling, in particular to a tumor tissue three-dimensional space sampler and a sampling method thereof.
Background
Tumors are abnormal lesions formed by the fact that under the action of various cancerogenic factors, a certain cell of local tissues loses the normal regulation of the growth of the cells at the gene level, and the cells cause the clonality abnormal hyperplasia of the cells.
Tumor heterogeneity is one of the characteristics of malignant tumors, and means that the tumor undergoes multiple division and proliferation in the growth process, and its daughter cells show changes in molecular biology or genes, so that the growth rate, invasion capacity, drug sensitivity, prognosis and other aspects of the tumor are different.
In practice, there are many kinds of tumors in different individuals (humans or animals), and the removed tumors in the same individual are different in different positions; in order to know the spatial structure and evolution process of the tumor in more detail and apply the most accurate treatment scheme to a tumor patient, the tumor sample taken from the body of the patient needs to be sampled at multiple points, then transcriptome, genome, proteome, pathological detection or other molecular detection is respectively carried out on the samples sampled at different points, the structure and evolution process of the tumor are researched from multiple levels, the problem of clinical tumor heterogeneity is solved, and the patient can take medicine more accurately.
In the multi-point sampling of tumor samples, tens or even hundreds of points are usually required to be taken, and in general, an operator needs to take down samples of each point one by one in operation, and then place the samples taken down by each point in corresponding squares. Wherein, because the tumor tissue is a precious sample with high requirements on time and preservation conditions, the point-by-point sampling mode wastes more time and adversely affects the freshness of the tumor tissue sampling.
Disclosure of Invention
In view of the above, the present invention provides a three-dimensional spatial sampler for tumor tissue and a sampling method thereof, which mainly aims to improve the efficiency of spatial sampling.
In order to achieve the above purpose, the present invention mainly provides the following technical solutions:
in one aspect, embodiments of the present invention provide a tumor tissue three-dimensional spatial sampler comprising:
a sampling assembly, comprising: the sampling platform is provided with a pressing part at the first end, a plurality of sampling needle tubes are arranged at the second end (loading) of the sampling platform, and two ends of a sampling channel in the sampling needle tubes are communicated with each other; wherein, in the length direction of the sampling needle tube, the outer wall of the sampling needle tube is provided with at least one section ring mark;
the sample separating assembly comprises a segment folder for cutting the segment ring mark.
The aim and the technical problems of the invention can be further realized by adopting the following technical measures.
Optionally, in the tumor tissue three-dimensional space sampler, the segment ring mark forms an annular groove on the outer wall of the sampling needle tube, and the annular groove is a continuous groove or an intermittent groove.
Optionally, the segments on the plurality of sampling needle tubes are arranged on horizontal planes at a plurality of height positions, and a plurality of segments are arranged in different horizontal planes, so that the segments on the plurality of sampling needle tubes are integrally arranged in a three-dimensional space.
Alternatively, the head of the sampling needle tube may have an inner horn shape.
Optionally, the aforementioned tumor tissue three-dimensional space sampler, wherein the sampling assembly further comprises:
and a sealing part of the port of the sampling needle tube.
Optionally, the tumor tissue three-dimensional space sampler described above, wherein the sealing component of the sampling needle tube port comprises a sealing pad.
Optionally, the tumor tissue three-dimensional space sampler is characterized in that the sealing backing plate is a silica gel plate.
Optionally, the aforementioned tumor tissue three-dimensional space sampler, wherein the sampling assembly further comprises:
an elastic backing plate.
Optionally, the tumor tissue three-dimensional space sampler, wherein the sample dividing assembly further comprises: pipe cutting pliers, segment holding pliers and sample separating spoon.
In another aspect, an embodiment of the present invention provides a sampling method of a tumor tissue three-dimensional space sampler, including:
sampling: aligning a plurality of sampling needle tubes at the second end of the sampling platform with a tissue sample, applying an external force to the pressing component, inserting the sampling needle tubes into the tissue sample (tumor tissue sample), and filling part of the tissue sample into sampling channels of the sampling needle tubes; then, applying external force to the pressing part, and pulling out the sampling needle tube from the tissue sample, so that part of the tissue sample poured into the sampling channel of the sampling needle tube is pulled out along with the sampling needle tube;
sample separation: the method comprises the steps of cutting a section ring mark fold of a (quasi) sampling needle tube by using a breaker, segmenting the sampling needle tube, sampling different sampling needle tube segments, and numbering and sample dividing according to the space position of the sampling needle tube where the segmented sampling needle tube is located.
The aim and the technical problems of the invention can be further realized by adopting the following technical measures.
Optionally, the foregoing sampling method, wherein the sampling assembly further comprises: the sampling needle tube port sealing component comprises a sealing backing plate;
the sampling step specifically comprises the following steps:
and (3) padding a sealing pad plate at the bottom of the tissue sample, aligning a plurality of sampling needle tubes at the second end of the sampling platform to the top of the tissue sample, applying external force to the pressing part, penetrating the end parts of the sampling needle tubes through the tissue sample, inserting the sealing pad plate into a sampling channel at the end parts of the sampling needle tubes, and sealing the tissue sample in the sampling channel of the sampling needle tubes.
Optionally, in the foregoing sampling method, the sample dividing assembly further includes: cutting pipe pliers, holding pliers and sample separating spoon;
the sample separation step specifically comprises the following steps:
shearing the rear end of the sampling needle tube by using a tube shearing pliers, and separating the sampling needle tube filled with the tissue sample part from the pressing part;
cutting the section ring mark fold of the sampling needle tube to a semi-separation state by using a breaker;
the section holding pliers are used for holding the folded section tube of the sampling needle tube in the semi-separated state and locking and picking the folded section tube;
the two ends of the folded section tube are respectively sampled by using a sample dividing spoon to serve as 2 samples, and the tissue sample remained in the middle of the folded section tube is taken as 1 sample.
By means of the technical scheme, the tumor tissue three-dimensional space sampler and the sampling method thereof provided by the technical scheme of the invention have at least the following advantages:
in the technical scheme provided by the embodiment of the invention, the first end of the sampling platform is provided with the pressing part, the second end of the sampling platform is provided with the plurality of sampling needle tubes, the outer wall of each sampling needle tube is provided with at least one section ring mark in the length direction of each sampling needle tube, and the sampling needle tubes can be divided into a plurality of sections by the section ring mark; in the process of sampling a tissue sample taken from an individual, applying an external force to the pressing component, and inserting a sampling needle tube into the tissue sample to enable part of the tissue sample to be poured into a sampling channel of the sampling needle tube; the invention has the advantages that compared with the existing manual single-point sampling technology, the time consumption is shorter, a plurality of samples on the tumor three-dimensional space can be taken at one time, and convenience is provided for researching the tumor space heterogeneity.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to designate like parts throughout the figures. In the drawings:
fig. 1 is a schematic top view of a sampling platform of a tumor tissue three-dimensional space sampler according to an embodiment of the present invention;
fig. 2 is a schematic diagram of a front view structure of a sampling platform of a three-dimensional tumor tissue sampler according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a schematic cross-sectional front view of a sampling platform of a three-dimensional tumor tissue sampler according to one embodiment of the present invention;
FIG. 4 is a schematic view of a sampling needle tube structure of a sampling platform of a three-dimensional tumor tissue sampler according to an embodiment of the present invention;
FIG. 5 is an enlarged view of the structure of FIG. 4A;
FIG. 6 is a schematic diagram of a front view of a segment folder of a tumor tissue three-dimensional space sampler according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of a three-dimensional space sampler for tumor tissue sampling according to an embodiment of the present invention;
fig. 8 is a schematic side view of a segment folding device of a tumor tissue three-dimensional space sampler according to an embodiment of the invention.
Detailed Description
In order to further describe the technical means and effects adopted by the invention to achieve the preset aim, the following detailed description refers to the specific implementation, structure, characteristics and effects of the tumor tissue three-dimensional space sampler and the sampling method thereof according to the invention by combining the accompanying drawings and the preferred embodiment. In the following description, different "an embodiment" or "an embodiment" do not necessarily refer to the same embodiment. Furthermore, the particular features, structures, or characteristics of one or more embodiments may be combined in any suitable manner.
Example 1
As shown in fig. 1, 2, 3, 4, 5 and 6, a three-dimensional tumor tissue sampler according to one embodiment of the present invention includes:
a sampling assembly, comprising: the sampling platform 10, a pressing part 11 is arranged at the first end of the sampling platform 10, a plurality of sampling needle tubes 12 are arranged at the second end of the sampling platform 10, the rear ends of the sampling needle tubes 12 are connected with the pressing part 11, the front ends of the sampling needle tubes 12 are used as needle heads sockets, and two ends of sampling channels in the sampling needle tubes 12 are communicated with each other; wherein, in the length direction of the sampling needle tube, the outer wall of the sampling needle tube is provided with at least one section ring mark 121, such as more than 2, more than 3, more than 4, etc.;
the sample separating assembly comprises a segment folder 21 for cutting the segment ring mark.
The process of sampling a tissue sample (cancer tissue removed from the patient's body) may be:
sampling: aligning a plurality of sampling needle tubes at the second end of the sampling platform with the tissue sample, applying external force to the pressing part, inserting the sampling needle tubes into the tissue sample, and filling part of the tissue sample into a sampling channel of the sampling needle tubes; then, applying external force to the pressing part, and pulling out the sampling needle tube from the tissue sample, so that part of the tissue sample poured into the sampling channel of the sampling needle tube is pulled out along with the sampling needle tube;
sample separation: the method comprises the steps of cutting a sectional ring mark fold of a sampling needle tube by using a breaker, segmenting the sampling needle tube, segmenting sampling different sampling needle tubes, and numbering and sampling according to the space position of the sampling needle tube where the segmented sampling needle tube is located.
In the technical scheme provided by the embodiment of the invention, the first end of the sampling platform is provided with the pressing part, the second end of the sampling platform is provided with the plurality of sampling needle tubes, the outer wall of each sampling needle tube is provided with at least one section ring mark in the length direction of each sampling needle tube, and the sampling needle tubes can be divided into a plurality of sections by the section ring mark; in the process of sampling a tissue sample taken from an individual, applying an external force to the pressing component, and inserting a sampling needle tube into the tissue sample to enable part of the tissue sample to be poured into a sampling channel of the sampling needle tube; the broken ring mark of the section of the sampling needle tube is cut by using the breaker, the sampling needle tube is segmented, different sampling needle tubes are sampled in a segmented mode, and the number and the sample separation are carried out according to the space position of the sampling needle tube where the segmented sampling needle tube is located. Meanwhile, the outer wall of the sampling needle tube is provided with the sectional ring marks, so that the whole sampling needle tube can be conveniently segmented, namely, the sectional ring marks on the whole sampling needle tube are broken one by one, and the whole sampling needle tube can be segmented. Compared with the mechanical cutting (such as using a machine tool, an electric cutting machine and a saw blade for cutting), a large amount of heat generated in the mechanical cutting process can be avoided, the high-temperature influence on the tissue sample in the sampling needle tube can be avoided, and meanwhile, the generated cutting dust can be prevented from being mixed into the tissue sample in the sampling needle tube in the mechanical cutting process.
At present, only one horizontal (two-dimensional) sample can be taken for tumor tissue sampling, and a spatial (three-dimensional) structure cannot be interpreted, so that three-dimensional sampling is realized:
the segments on the plurality of sampling needle tubes are arranged on horizontal planes at a plurality of height positions, and a plurality of segments are arranged in different horizontal planes, so that the segments on the plurality of sampling needle tubes are integrally arranged in a three-dimensional space. Wherein each segment represents a segment which is taken down after the ring mark of the segment on the sampling needle tube is broken, and 1 segment can be taken down after each broken ring mark of the segment on the sampling needle tube; in the implementation, the plurality of sampling needle tubes can be arranged horizontally and longitudinally at equal intervals, the corresponding pressing parts are rectangular, or the root sampling needle tubes can be arranged circumferentially, and the corresponding pressing parts are circular or semicircular; wherein the interval between each sampling needle tube can be set according to the sampling interval distance. Of course, the plurality of sampling needle tubes may be arranged in a non-equidistant manner, as desired. Similarly, the number of ring segments on each sampling needle tube may be multiple, and the distance between each ring segment may be equal or unequal. The number of segmented ring marks on different sampling needle tubes may be equal or unequal. In the tumor tissue sampling tool in the prior art, only one horizontal (two-dimensional) sampling operation can be realized, but in the invention, the segments on the plurality of sampling needle tubes are integrally arranged in a three-dimensional space, and in the process of sampling a tumor tissue sample, namely, the sampling needle tubes are inserted into the tissue sample, so that part of the tissue sample is poured into a sampling channel of the sampling needle tubes; and after the sampling needle tube is pulled out of the tissue sample, the three-dimensional space sampling operation of the tumor tissue sample can be performed at one time.
Wherein, many sample needle tubing are straight needle tubing, and are parallel each other.
In order to realize the convenient cutting of the section ring mark, the section ring mark is formed into an annular groove on the outer wall of the sampling needle tube, and the annular groove is a continuous groove or an intermittent groove. The grooves can be U-shaped grooves, V-shaped grooves and the like, and the intermittent grooves can be formed by enclosing point grooves or enclosing a plurality of grooves.
To facilitate alignment of the sampling platform on the tissue sample, the pressing member may have an alignment reference, such as a notch, on one side of the pressing member for alignment.
The pressing part and the plurality of sampling needle tubes can be connected in a separable way, the pressing part can be made of a transparent sub-force gram plate, the sampling needle tubes can be stainless steel needles, the pressing part and the plurality of sampling needle tubes can be connected in a fixed integrated way, and the pressing part and the plurality of sampling needle tubes are made of stainless steel materials.
The diameter of the sampling needle tube is determined according to the amount of sampling required, such as a needle with the diameter of 1.5-3 mm;
as shown in FIG. 5, in order to facilitate the sampling of the head of the sampling needle, the head of the sampling needle may be provided with an inner horn 122, the wall thickness of the head of the sampling needle may be gradually thickened from the end portion to the inner direction, and when the sampling needle is inserted into the tissue sample, a part of the tissue sample may be poured into the sampling passage of the sampling needle, and the horn may facilitate the cancer tissue to enter the sampling needle.
In addition, the head of the sampling needle tube can be in a bevel shape so as to facilitate insertion and increase the pressure of the sampling vacuum end.
After a portion of the tissue sample is poured into the sampling channel of the sampling needle cannula, in order to firmly hold the tissue sample in the sampling channel when the sampling needle cannula is pulled out of the tissue sample, the sampling assembly further comprises: and a sealing part of the port of the sampling needle tube. Before the sampling needle tube is pulled out from the tissue sample, the sealing part is plugged at any one end or two ends of the sampling needle tube.
As shown in FIG. 7, in particular, the sample needle cannula port seal assembly may include a seal backing plate 30. In the operation sampling step, the specific steps are as follows:
the sealing pad is padded at the bottom of the tissue sample, the plurality of sampling needle tubes at the second end of the sampling platform are aligned to the top of the tissue sample, external force is applied to the pressing component, the end parts of the sampling needle tubes penetrate through the tissue sample and are inserted into the sealing pad, so that the (partial) sealing pad is embedded into a sampling channel at the end part of the sampling needle tube, and the tissue sample in the sampling channel of the sampling needle tube is sealed (the sealing pad is embedded into the sampling channel at the end part of the sampling needle tube, and the tissue sample in the sampling channel of the sampling needle tube is sealed, namely, the partial sealing pad is sealed in the front end of the sampling needle tube). Specifically, the sealing backing plate can be a silica gel plate, a rubber plate and the like.
In order to reduce the likelihood of the sample needle buckling within the tissue sample z1 as a result of excessive force applied to the compression member, the sampling assembly further comprises: and an elastic pad 40.
The sampling step specifically comprises the following steps:
the sealing pad plate is arranged at the bottom of the tissue sample, the elastic pad plate is arranged at the bottom of the sealing pad plate, the plurality of sampling needle tubes at the second end of the sampling platform are aligned to the top of the tissue sample, external force is applied to the pressing part, the end parts of the sampling needle tubes penetrate through the tissue sample, the sealing pad plate is inserted, the sealing pad plate is embedded into a sampling channel at the end parts of the sampling needle tubes, and the tissue sample in the sampling channel of the sampling needle tubes is sealed.
Wherein, in exerting external force to the pressing component, the sampling needle tube passes through the tissue sample, the sealing pad and the elastic pad (penetrating sound is heard) and then stops pressing down.
The elastic pad can be a foam board (such as KT board, which is a board core formed by foaming PS particles, and the main component is polystyrene), a spring pad and the like.
Specifically, the sample separation assembly further comprises: pipe cutting pliers, segment holding pliers and sample separating spoon.
Pipe cutting pliers: the pipe cutting pliers adopt small-size nozzle pliers and have the function of separating the sampled sampling needle pipes from the pressing part one by utilizing shearing force.
As shown in fig. 6 and 8, the segment folder 21: the segment folder 21 is similar to a guillotine in structure and consists of a cutter frame 211 and a connecting handle 212 without a blade. When the sampling needle tube is used, the sampling needle tube separated from the pressed component is held on the left hand, the sampling needle tube is placed on the tool rest, the sectional ring mark on the sampling needle tube is aligned to the tool edge, the tool handle is pressed down by the right hand with proper force, after the breaking sound is heard, one section of the sampling needle tube is broken, and the section of the sampling needle tube is temporarily adhered to the whole held on the left hand.
Segment holding pliers: the holding section forceps can be hemostatic forceps with elbows. The method is used in two links of folding tube sample separation and scooping out sample tissue respectively. When the segment holding pliers are used in the step of sample separation, the segment holding pliers are held by the right hand, the bend is directed to the left, the segment is clamped, the segment is locked and lightly forced, and the extracted segment and the sample tissues therein are temporarily fixed on the segment holding pliers.
Sample separating spoon: the shape of the sample separating spoon is similar to that of a small spoon and an elbow. The purpose is to scoop out the sample tissue in the cut-off of the sampling needle tube.
The sample separation step can be specifically as follows:
shearing the rear end of the sampling needle tube by using a tube shearing pliers, and separating the sampling needle tube filled with the tissue sample part from the pressing part;
cutting the section ring mark fold of the sampling needle tube to a semi-separation state by using a breaker;
the section holding pliers are used for holding the folded section tube of the sampling needle tube in the semi-separated state and locking and picking the folded section tube;
the two ends of the folding section tube are respectively sampled to be 2 samples by using a sample dividing spoon, the rest tissue samples in the middle of the folding section tube are taken as 1 sample, and each folding section tube of the sampling needle tube is carved with an up-down azimuth mark so as to be convenient for distinguishing the up-down 2 samples.
Examples
A standard type common long tumor tissue three-dimensional space sampler: the sampling platform has 24 sampling needle tubes, and the sampling needle tube is the stainless steel pipe of diameter 1.5 mm, and sampling needle tube length 25 mm, front end mill out the blade, and the section ring trace has 7. The sampling needle tube can be folded into 7 sections (each section is about 3mm long, and 3 sample data points can be totally divided from the top to the bottom of each section), and each section is marked with an up-down azimuth mark. 3mm thick silica gel plate, 5mm thick KT plate.
Three-dimensional space sampler of standard extension formula tumor tissue: the sampling platform is provided with 24 sampling needle tubes, the sampling needle tubes are stainless steel tubes with the diameter of 1.5 mm, the sampling needle tubes are 38 mm long, cutting edges are ground at the front ends, and 12 sections of ring marks are formed. The sampling needle tube can be folded into 12 sections (each section is about 3mm long, and 3 sample data points can be totally divided from the top to the bottom of each section), and each section is marked with an up-down azimuth mark. 3mm thick silica gel plate, 5mm thick KT plate.
Special type 1 general long tumor tissue three-dimensional space sampler: the sampling platform has 15 sampling needle tubes, and the sampling needle tube is the stainless steel pipe of diameter 1.5 mm, and sampling needle tube length 25 mm, front end mill out the blade, and the section ring trace has 7. The sampling needle tube can be folded into 7 sections (each section is about 3mm long, and 3 sample data points can be totally divided from the top to the bottom of each section), and each section is marked with an up-down azimuth mark. 3mm thick silica gel plate, 5mm thick KT plate.
Special 2-type common long tumor tissue three-dimensional space sampler: the sampling platform has 20 sampling needle tubes, and the sampling needle tube is the stainless steel pipe of diameter 1.5 mm, and sampling needle tube length 25 mm, front end mill out the blade, and the section ring trace has 7. The sampling needle tube can be folded into 7 sections (each section is about 3mm long, and 3 sample data points can be totally divided from the top to the bottom of each section), and each section is marked with an up-down azimuth mark. 3mm thick silica gel plate, 5mm thick KT plate.
Example two
According to the sampling method provided by the second embodiment of the invention, the tumor tissue three-dimensional space sampler described in the first embodiment is adopted, and the method comprises the following steps:
sampling: aligning a plurality of sampling needle tubes at the second end of the sampling platform with the tissue sample, applying external force to the pressing part, inserting the sampling needle tubes into the tissue sample, and filling part of the tissue sample into a sampling channel of the sampling needle tubes; then, applying external force to the pressing part, and pulling out the sampling needle tube from the tissue sample, so that part of the tissue sample poured into the sampling channel of the sampling needle tube is pulled out along with the sampling needle tube;
the tissue samples are required to be distinguished in the directions of up, down, left and right, and when the tissue samples are placed, the tissue samples are required to be aligned and placed according to the directions required to be detected; and applying external force to the pressing part, wherein in the process of inserting the sampling needle tube into the tissue sample, bare-handed operation can be adopted, or an external pressure device can be adopted, pressure is applied to a handle of the pressure device, and the pressure is amplified through a gear and a rack of the pressure device and then acts on the pressing part, so that the sampling needle tube is smoothly inserted into the tissue sample.
Sample separation: the method comprises the steps of cutting a sectional ring mark fold of a sampling needle tube by using a breaker, segmenting the sampling needle tube, segmenting sampling different sampling needle tubes, and numbering and sampling according to the space position of the sampling needle tube where the segmented sampling needle tube is located.
Specific:
the sampling assembly further comprises: the sampling needle tube port sealing component comprises a sealing backing plate;
the sampling step specifically comprises the following steps:
and (3) padding a sealing pad plate at the bottom of the tissue sample, aligning a plurality of sampling needle tubes at the second end of the sampling platform to the top of the tissue sample, applying external force to the pressing part, penetrating the end parts of the sampling needle tubes through the tissue sample, inserting the sealing pad plate into a sampling channel at the end parts of the sampling needle tubes, and sealing the tissue sample in the sampling channel of the sampling needle tubes.
The tumor tissue three-dimensional space sampler can be provided with various types and various lengths of sampling needle tubes; when the model is selected, the sampling needle tube with the corresponding length can be selected according to the thickness of the tissue sample, so that the length of the sampling needle tube at one side of the sampling platform is 1-3cm greater than the thickness of the tissue sample.
Specific:
the sample separation assembly further comprises: cutting pipe pliers, holding pliers and sample separating spoon;
the sample separation step specifically comprises the following steps:
shearing the rear end of the sampling needle tube by using a tube shearing pliers, and separating the sampling needle tube filled with the tissue sample part from the pressing part;
specifically, in the process of separating each sampling needle tube from the pressing component, each separated sampling needle tube is placed in a low temperature for preservation, and quick freezing is carried out in the low temperature;
cutting the section ring mark fold of the sampling needle tube to a semi-separation state by using a breaker;
the section holding pliers are used for holding the folded section tube of the sampling needle tube in the semi-separated state and locking and picking the folded section tube;
the method comprises the steps of cutting a section ring mark fold of a sampling needle tube to a semi-separation state by using a breaker; the step of clamping the folded section tube of the sampling needle tube in the semi-separated state by using the section clamping pliers and locking and taking off is needed to be operated on ice so as to keep the freshness of the tissue sample.
The two ends of the folded section tube are respectively sampled by using a sample dividing spoon to serve as 2 samples, the tissue sample remained in the middle of the folded section tube serves as 1 sample, and 3 samples are respectively placed in corresponding centrifuge tubes and numbered (namely the centrifuge tubes are numbered).
The following numbering mode can be adopted for the multiple sampling needle tubes of the tumor tissue three-dimensional space sampler so as to distinguish each sampling needle tube, and the segment and the point position of each sampling needle tube:
numbering each sampling needle tube respectively;
the segments on each sampling needle tube are respectively numbered;
each segment is numbered up, down, and middle.
Taking 24 sampling needle tubes arranged at equal intervals in a 5-horizontal and 5-longitudinal mode as an example:
numbering structure: sampling needle row-sampling needle column-sampling needle section (segment) -sampling needle section point
Row: when numbering, the plurality of sampling needle tubes are aligned in a good plane direction according to the working state (the pressing part is arranged on the upper part, the direction alignment reference of the pressing part is aligned with an operator), the first transverse row in front of the sampling needle tubes is 1, the second transverse row is 2 … …, and the row farthest from the operator is 5.
The columns are: the right side of the first transverse row is provided with a sampling tube from 1 to 1, the second sampling tube from 1 to 2 is arranged from left to right, the first transverse row is not provided with a number of 1 to 3 due to structural reasons, and the leftmost sampling tube of the first transverse row is provided with a number of 1 to 4; the rightmost root of the second horizontal row is 2-1, and the leftmost root is 2-5; by analogy, the leftmost root of the row furthest from the operator is "5-5".
Section: starting from the lowest section of the sampling needle tube from bottom to top, the first section of the leftmost strip cutting edge of the first horizontal row is 1-4-1, and the second section is 1-4-2; by analogy, section 5 of the 2 nd root of the third row from right to left is "3-2-5".
The point: for scooping sample tissue from a section of a sampling tube using a dispensing spoon, imprinted on the surface of the section with the arrow pointing downwards, the scooped tissue being "a" on the lower side and "c" on the upper side, and finally "b" left in the tube.
Such as: the sample tissue is in the position from the nearest first row of the operator, from the right of row 4, from the bottom of row 3 to the top of section 5, and from the top of the spoon.
Of course, the numbering method is just a specific way, and the specific numbering can be performed in any way.
Specifically, the tumor tissue three-dimensional space sampler described in the second embodiment may directly use the tumor tissue three-dimensional space sampler provided in the first embodiment, and specific implementation structures may refer to the related contents described in the first embodiment, which are not described herein again.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to related descriptions of other embodiments.
It will be appreciated that the relevant features of the apparatus described above may be referred to with respect to each other. In addition, the "first", "second", and the like in the above embodiments are for distinguishing the embodiments, and do not represent the merits and merits of the embodiments.
In the description provided herein, numerous specific details are set forth. However, it is understood that embodiments of the invention may be practiced without these specific details. In some instances, well-known structures and techniques have not been shown in detail in order not to obscure an understanding of this description.
Similarly, it should be appreciated that in the foregoing description of exemplary embodiments of the invention, various features of the invention are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the disclosure and aiding in the understanding of one or more of the various inventive aspects. However, the disclosed apparatus should not be construed as reflecting the intention of: i.e., the claimed invention requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this invention.
Those skilled in the art will appreciate that the components of the apparatus of the embodiments may be adaptively changed and disposed in one or more apparatuses different from the embodiments. The components of the embodiments may be combined into one component, and furthermore they may be divided into a plurality of sub-components. All of the features disclosed in this specification (including any accompanying claims, abstract and drawings), and all of the elements of any apparatus so disclosed, may be combined in any combination, except combinations where at least some of such features are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, abstract and drawings), may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise.
Furthermore, those skilled in the art will appreciate that while some embodiments described herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments. For example, in the following claims, any of the claimed embodiments can be used in any combination. Various component embodiments of the present invention may be implemented in hardware, or in a combination thereof.
It should be noted that the above-mentioned embodiments illustrate rather than limit the invention, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference signs placed between parentheses shall not be construed as limiting the claim. The word "comprising" does not exclude the presence of elements or components not listed in a claim. The word "a" or "an" preceding a component or assembly does not exclude the presence of a plurality of such components or assemblies. The invention may be implemented by means of an apparatus comprising several distinct elements. In the claims enumerating several means, several of these means may be embodied by one and the same item of hardware. The use of the words first, second, third, etc. do not denote any order. These words may be interpreted as names.
The above description is only of the preferred embodiments of the present invention, and is not intended to limit the present invention in any way, but any simple modification, equivalent variation and modification made to the above embodiments according to the technical substance of the present invention still fall within the scope of the technical solution of the present invention.
Claims (11)
1. A tumor tissue three-dimensional spatial sampler, comprising:
a sampling assembly, comprising: the sampling platform is provided with a pressing part at the first end, a plurality of sampling needle tubes are arranged at the second end, the sections on the plurality of sampling needle tubes are arranged on horizontal planes at a plurality of height positions, and a plurality of sections are arranged in different horizontal planes, so that the sections on the plurality of sampling needle tubes are integrally arranged in a three-dimensional space, and the two ends of a sampling channel in the sampling needle tube are mutually communicated; wherein, in the length direction of the sampling needle tube, the outer wall of the sampling needle tube is provided with at least one section ring mark;
the sample separating assembly comprises a segment folder for cutting the segment ring mark.
2. The tumor tissue three-dimensional space sampler of claim 1 wherein,
the section ring mark is formed into an annular groove on the outer wall of the sampling needle tube, and the annular groove is a continuous groove or an intermittent groove.
3. The tumor tissue three-dimensional space sampler of claim 1 wherein,
the head of the sampling needle tube adopts an inner horn shape.
4. The tumor tissue three-dimensional space sampler of claim 1 wherein,
the sampling assembly further comprises:
and a sealing part of the port of the sampling needle tube.
5. The tumor tissue three-dimensional space sampler of claim 4 wherein,
the port sealing component of the sampling needle tube comprises a sealing backing plate.
6. The tumor tissue three-dimensional space sampler of claim 5 wherein,
the sealing backing plate is a silica gel plate.
7. The tumor tissue three-dimensional space sampler of claim 5 wherein,
the sampling assembly further comprises:
an elastic backing plate.
8. The tumor tissue three-dimensional space sampler of claim 1 wherein,
the sample separation assembly further comprises: pipe cutting pliers, segment holding pliers and sample separating spoon.
9. A sampling method using the tumor tissue three-dimensional space sampler of any one of claims 1-8, comprising:
sampling: aligning a plurality of sampling needle tubes at the second end of the sampling platform with the tissue sample, applying external force to the pressing part, inserting the sampling needle tubes into the tissue sample, and filling part of the tissue sample into a sampling channel of the sampling needle tubes; then, applying external force to the pressing part, and pulling out the sampling needle tube from the tissue sample, so that part of the tissue sample poured into the sampling channel of the sampling needle tube is pulled out along with the sampling needle tube;
sample separation: the method comprises the steps of cutting a sectional ring mark of a sampling needle tube by using a section folding device, segmenting the sampling needle tube, sampling different sampling needle tube segments, and numbering and sample dividing according to the space position of the sampling needle tube where the segmented sampling needle tube is located.
10. The sampling method according to claim 9, wherein the sampling method comprises,
the sampling assembly further comprises: the sampling needle tube port sealing component comprises a sealing backing plate;
the sampling step specifically comprises the following steps:
and (3) filling the sealing backing plate at the bottom of the tissue sample, aligning a plurality of sampling needle tubes at the second end of the sampling platform with the top of the tissue sample, applying external force to the pressing part, penetrating the end parts of the sampling needle tubes through the tissue sample, inserting the sealing backing plate into the sampling channels at the end parts of the sampling needle tubes, embedding the sealing backing plate into the sampling channels at the end parts of the sampling needle tubes, and sealing the tissue sample in the sampling channels of the sampling needle tubes.
11. The sampling method according to claim 9, wherein the sampling method comprises,
the sample separation assembly further comprises: cutting pipe pliers, holding pliers and sample separating spoon;
the sample separation step specifically comprises the following steps:
shearing the rear end of the sampling needle tube by using a tube shearing pliers, and separating the sampling needle tube filled with the tissue sample part from the pressing part;
cutting the section ring mark of the sampling needle tube to a semi-separation state by using a section folding device;
the section holding pliers are used for holding the folded section tube of the sampling needle tube in the semi-separated state and locking and picking the folded section tube;
the two ends of the folded section tube are respectively sampled by using a sample dividing spoon to serve as 2 samples, and the tissue sample remained in the middle of the folded section tube is taken as 1 sample.
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| CN111839596B (en) * | 2020-06-12 | 2022-05-13 | 珠海市人民医院 | Clamping type conveniently-folded multi-section sampling rod |
| CN113063620B (en) * | 2021-03-25 | 2022-06-10 | 陕西省肿瘤医院 | Tumor sampling device convenient for tumor separation |
| CN116296687B (en) * | 2023-03-31 | 2024-09-10 | 中国中医科学院医学实验中心 | Tissue sample preparation device and application thereof |
| CN118750045B (en) * | 2024-07-10 | 2025-01-24 | 南通大学附属医院 | A tumor sampling device for easily separating tumors |
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