CN106538531B - The application of Fusarium oxysporum fungicide and citral - Google Patents
The application of Fusarium oxysporum fungicide and citral Download PDFInfo
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- CN106538531B CN106538531B CN201610917551.3A CN201610917551A CN106538531B CN 106538531 B CN106538531 B CN 106538531B CN 201610917551 A CN201610917551 A CN 201610917551A CN 106538531 B CN106538531 B CN 106538531B
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- citral
- fusarium oxysporum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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Abstract
本发明涉及一种尖孢镰刀菌杀菌剂，活性成分包括柠檬醛。本发明还涉及一种柠檬醛的应用，将该柠檬醛作为活性成分，用于防治尖孢镰刀菌。
The invention relates to a fungicide for Fusarium oxysporum, and the active ingredient includes citral. The invention also relates to an application of citral, which is used as an active ingredient for preventing and treating Fusarium oxysporum.
Description
Technical field
The present invention relates to root rot prevention and control field, in particular to the application of a kind of Fusarium oxysporum fungicide and citral.
Background technique
Root-rot disease is most one of universal, the maximum disease of harmfulness in arable farming, by Chinese medicine root rot disease
After opportunistic pathogen carries out identification research, discovery Fusarium oxysporum is main pathogenic bacteria.Fusarium oxysporum (fusarium oxysporium)
For Fungi Imperfecti Fusarium fungi.Cause of disease is overwintering in the soil or in old complaint with mycelia, becomes source of infection again.Root rot master
Cause harm seedling root or basal part of stem, can start to fall ill after pathogen field planting, plant catches an illness initial stage, only in individual supporting roots and fibrous root
It catches an illness, and water stain shape occurs.Subsequent lesion is gradually extended to main root, after main root is susceptible, plant early stage performance symptom it is unobvious or
Asymptomatic appearance.Thereafter with the aggravation of butt rot degree, epiblem presentation is brown to rot to dark brown, and historrhexis is only stayed
Lower filiform brown vascular bundle causes its absorption moisture and nutrient miopragia, ground position to obtain not since root epidermis is destroyed
To abundant nutrient, plant shows the micro- volume of the tip of a root and upwarps, slow growth, the symptoms such as young leaves jaundice, to directly affect Chinese medicine
Yield and quality, cause huge economic loss.Currently, chemical pesticide is quick-acting to harmful organism, easy to operate and suitable because of it
The problems such as the features such as Ying Xingguang is widely used in the prevention and treatment of root rot, opposite, Pesticide use confusion, high poison high residue is increasingly prominent
Out.
Summary of the invention
In view of this, it is necessory to provide a kind of Fusarium oxysporum fungicide and citral safely, effectively, less toxic
Application.
A kind of Fusarium oxysporum fungicide, active constituent include citral.
A kind of application of citral, using the citral as active constituent, for preventing and treating Fusarium oxysporum.
The invention proposes the new applications of citral prevention and treatment Chinese medicine root-rot pathogen Fusarium oxysporum, which can be with
For Fusarium oxysporum fungicide.Citral is a kind of terpenoid, colourless or yellowish liquid, is in citrus scented, easily mentions
It takes, is naturally present in lemongrass oil, litsea citrate oil, in lemon oil, citrus leaf oil, in addition, from industrial geraniol (and flores aurantii
Alcohol) with ketone catalyst decompression gas-phase dehydrogenation can draw citral.Citral is dissolved in oils, propylene glycol and ethyl alcohol, does not dissolve in glycerol
And water.Citral is nontoxic, edible, thus using citral prevent and treat Pathogens Causing Root Rot Disease Fusarium oxysporum, have safely, have
The effect of effect, low toxicity.
Detailed description of the invention
Fig. 1 is the test experience schematic diagram to Fusarium oxysporum bacteriostasis.
Fig. 2 is fungistatic effect test data figure of the citral solution to Fusarium oxysporum.
Fig. 3 to Fig. 7 is fungistatic effect photo of the citral solution to Fusarium oxysporum of various concentration.
Specific embodiment
Below with reference to the application of attached drawing the present invention will be described in detail embodiment Fusarium oxysporum fungicide and citral.
The embodiment of the present invention provides a kind of Fusarium oxysporum fungicide, and the active constituent of the fungicide includes citral.?
In the fungicide, citral can be separately as active constituent.In other embodiments, which can also wrap
Other active components are included, which can carry out compatibility with the other active components and prevent and treat Fusarium oxysporum jointly.
The Fusarium oxysporum fungicide is specifically as follows citral solution, including citral and solvent, citral dissolution
Or it is dispersed in the solvent.As long as the solvent can dissolve or disperse the citral, and for citral to Fusarium oxysporum
Bacteriostasis not adversely affect, can be common solvent, such as water, ethyl alcohol, methanol and dimethyl sulfoxide (DMSO)
At least one of.In a preferred embodiment, it is fullyd shake using concussion instrument, mixes citral and water, form dispersion liquid, it should
Fusarium oxysporum fungicide is that citral is dispersed in water the dispersion liquid to be formed.Since citral is insoluble and volatile in water,
Solution is formed in organic solvent relative to dissolution, experiments have shown that there is citral dispersion liquid the sterilization of better Fusarium oxysporum to make
With.
Concentration of the citral in the fungicide is preferably greater than 4gL-1, more preferably higher than 10gL-1。
The embodiment of the present invention also provides a kind of Fusarium oxysporum fungicide, and the Fusarium oxysporum fungicide is by the citral
And the solvent composition.
The embodiment of the present invention also provides a kind of application of citral, specially using the citral as active constituent, is used for
Prevent and treat Fusarium oxysporum.In application citral prevention and treatment Fusarium oxysporum, which dissolves in a solvent, forms citral
Solution.
The citral solution includes citral and solvent, which is dissolved or dispersed in the solvent.As long as the solvent
The citral can be dissolved or be dispersed, and bacteriostasis of the citral to Fusarium oxysporum is not adversely affected,
It can be common solvent, such as at least one of water, ethyl alcohol, methanol and dimethyl sulfoxide (DMSO).The citral is as work
Property ingredient, the concentration for preventing and treating Fusarium oxysporum is preferably greater than 4gL-1, more preferably higher than 10gL-1。
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.In the embodiment of the present invention
Used material and reagent, are commercially available unless otherwise specified.
Embodiment 1-8
It is 4,6,8,10gL that citral is made into concentration in water-1Dispersion liquid, respectively obtain 4gL-1Citral point
Dispersion liquid (fungicide 1), 6gL-1Citral dispersion liquid (fungicide 2), 8gL-1Citral dispersion liquid (fungicide 3), 10gL-1Citral dispersion liquid (fungicide 4).
Fusarium oxysporum used is saved by Institute Of Chinese Materia Medica Of China Academy of Chinese Medical Sciences.Using fungicide 1-4 to sharp spore reaping hook
Bacterium carries out bacteriostasis test experience, verifies influence of the citral solution of various concentration to Fusarium oxysporum.
Specifically, potato dextrose agar (PDA) medium liquid for taking 20mL to sterilize is added in sterile petri dish, to
The 1 × 10 of 400 μ L are drawn after liquid solidification8cfu·ml-1Fusarium oxysporum uniform suspension is coated on the PDA of solidification, simultaneously
The sterilizing filter paper disk that 4 diameters are 8mm is uniformly sticked in culture dish.Referring to Fig. 1, A indicates sterilizing filter paper disk, B table
Show Fusarium oxysporum applying area.4 kinds of culture dishes are made using fungicide 1-4, are dripped on 4 sterilizing filter paper disks of every kind of culture dish
Add identical fungicide.
Comparative example
Culture dish of the production for control, production method is identical as embodiment 1-4, and difference is only that, 4 of culture dish are gone out
Water is only added dropwise on bacterium filter paper dick.
Experimental result
Embodiment 1-4 and the culture dish of comparative example are placed in 28 DEG C of incubator culture same times, and specially 2 to 3 days.To right
Ratio bacterium colony covers with the plate of culture dish, carries out experimental result record.Every the retest of clock culture dish 3 times.It has observed and recorded
No antibacterial band generates and measures Fusarium oxysporum colony diameter, and experimental result is as shown in table 1 and Fig. 2-Fig. 3.An average column in table 1
For the average value of the antibacterial circle diameter of 3 repeated experiments.
Fungistatic effect of the 1. citral dispersion liquid of table to Fusarium oxysporum
Fig. 3 to Fig. 7 is please referred to, the culture dish pickup light to embodiment 1-4 and comparative example after culture same time is passed through
Photo is learned, fungistatic effect of the citral solution to Fusarium oxysporum of various concentration can be more intuitively observed.Fig. 3 to Fig. 7 with
The corresponding relationship of culture dish is as shown in table 2.
2. culture dish of table and Fig. 3 are to Fig. 7 mapping table
It can see from above-mentioned experimental data, fungicide provided in an embodiment of the present invention is to Pathogens Causing Root Rot Disease point spore reaping hook
Bacterium has bacteriostasis, and inhibitory effect is related with the concentration of citral.The criterion of antibacterial effect is arranged are as follows: antibacterial circle is straight
It is extremely sensitive that diameter, which is greater than 20mm,；15-20mm is high sensitive；10-15mm is middle sensitivity；7-9mm is muting sensitive sense；It is not less than 7mm
It is sensitive.From experimental result it can be seen that, when in fungicide lemon aldehyde concentration be greater than or equal to 4gL-1And it is less than 10gL-1When,
Antibacterial circle diameter is about 10-12mm, shows as middle sensitivity.When lemon aldehyde concentration is greater than or equal to 10gL in fungicide-1When, training
It is sterile on feeding ware to drop out now, it shows as extremely sensitive.Comparative example water shows as pathogen insensitive, the results showed that citral is to root
Maize ear rot opportunistic pathogen Fusarium oxysporum has inhibiting effect.
Root-rot disease fungus Fusarium oxysporum, efficient, low toxicity, when the concentration of citral is greater than are prevented and treated using citral solution
Or it is equal to 4gL-1And it is less than 10gL-1When, antibacterial circle diameter 10-12mm shows as middle sensitivity.When the concentration of citral
More than or equal to 10gL-1When, it is sterile on culture dish to drop out existing, it shows as extremely sensitive.
The embodiment of the present invention proposes the new application of citral prevention and treatment Chinese medicine root-rot pathogen Fusarium oxysporum, the lemon
Aldehyde can be used for Fusarium oxysporum fungicide.Citral is a kind of terpenoid, colourless or yellowish liquid, is in lemonene
Taste easily extracts, is naturally present in lemongrass oil, litsea citrate oil, in lemon oil, citrus leaf oil, in addition, from industrial geraniol
(and nerol), which depressurizes gas-phase dehydrogenation with ketone catalyst, can draw citral.Citral is nontoxic, edible, therefore uses citral
Prevent and treat Pathogens Causing Root Rot Disease Fusarium oxysporum, have the effect of safely, effectively, it is less toxic.Citral solution is to root-rot pathogen point
Fusarium oxysporum has inhibiting effect.When lemon aldehyde concentration is greater than 10gL-1When, it is extremely sensitive to pathogen, it completely inhibits on culture dish
Bacterium colony growth, has good control efficiency.
In addition, those skilled in the art can also do other variations in spirit of that invention, certainly, these are smart according to the present invention
The variation that mind is done, all should be comprising within scope of the present invention.
Claims (2)
1. a kind of Fusarium oxysporum fungicide, which is characterized in that including solvent and active constituent, the active constituent is lemon
Aldehyde, the solvent are water, and the citral is dispersed in the water, and the concentration of the citral is greater than or equal to 10gL-1。
2. a kind of Fusarium oxysporum fungicide as described in claim 1 is for preventing and treating Fusarium oxysporum.
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| CN201610917551.3A CN106538531B (en) | 2016-10-20 | 2016-10-20 | The application of Fusarium oxysporum fungicide and citral |
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| CN106538531B true CN106538531B (en) | 2019-04-16 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520258A (en) * | 2001-04-17 | 2004-08-11 | ������������ʽ���� | Bactericide compsn. |
| CN102369956A (en) * | 2011-10-18 | 2012-03-14 | 浙江农林大学 | Plant essential oil containing agricultural fungicide and sterilization applications thereof |
| CN103494886A (en) * | 2013-08-19 | 2014-01-08 | 浙江农林大学 | Spray type beriberi liquid containing vinegar liquid and essential oil of bamboo and wood, nano-grade manufacturing method, and applications thereof |
| CN103609667A (en) * | 2013-12-10 | 2014-03-05 | 浙江师范大学 | Comprehensive preservation method for waxberries by using plant extracts |
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2016
- 2016-10-20 CN CN201610917551.3A patent/CN106538531B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520258A (en) * | 2001-04-17 | 2004-08-11 | ������������ʽ���� | Bactericide compsn. |
| CN102369956A (en) * | 2011-10-18 | 2012-03-14 | 浙江农林大学 | Plant essential oil containing agricultural fungicide and sterilization applications thereof |
| CN103494886A (en) * | 2013-08-19 | 2014-01-08 | 浙江农林大学 | Spray type beriberi liquid containing vinegar liquid and essential oil of bamboo and wood, nano-grade manufacturing method, and applications thereof |
| CN103609667A (en) * | 2013-12-10 | 2014-03-05 | 浙江师范大学 | Comprehensive preservation method for waxberries by using plant extracts |
Non-Patent Citations (1)
| Title |
|---|
| 山鸡椒挥发油成分分析及其抗真菌保鲜作用的研究;黄粱绮龄等;《天然产物研究与开发》;19941231;第6卷(第4期);第1-5页 * |
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