CN106511198A - Sanguis Draconis extract, and preparation method and application thereof - Google Patents

Abstract

The invention discloses a dragon's blood extract, a preparation method and application thereof. The preparation method of the dragon's blood extract comprises the following steps: using an organic solvent to extract the dragon's blood, and the obtained extract is the dragon's blood extract; the organic solvent is polyhydric alcohol or white oil. The present invention overcomes the problems in the prior art of using organic solvents such as ethanol, methanol, acetone, and ethyl acetate to extract dragon's blood, and the extraction rate of the extract is not high or needs to be concentrated before being added to cosmetics, which has poor compatibility with the cosmetic base and is stable. Poor sex, high allergenicity and other issues. The dragon's blood extract of the present invention has good compatibility with the cosmetic matrix, and can be directly added to cosmetics; the product has good stability, and is stable against high temperature, low temperature, light and other harsh conditions; the extraction rate is high, and the loss of the dragon's blood raw material is small , reaching the maximum utilization of the raw material of dragon's blood. In addition, in addition to anti-oxidation and anti-aging effects, it also has significant acne-removing and whitening effects.

Description

A kind of dragon's blood extract and its preparation method and application

technical field

The invention relates to a traditional Chinese medicine extract and its preparation method and application, in particular to a dragon's blood extract and its preparation method and application.

Background technique

Dracaena cochinchinensis (Lour.) S.C.Chen is a resin extracted from the lipid-containing wood of the Liliaceae plant, Dranaena cochinchinensis (Lour.) S.C. Chen. It is mainly produced in Guangxi and Yunnan. "Compendium of Materia Medica" records that it has the characteristics of warm and flat, sweet and salty, and non-toxic. Its main functions are promoting blood circulation, removing blood stasis, astringent and hemostasis. The chemical composition of dragon's blood is very complex, including flavonoids, simple phenols, steroids, terpenes, hydrocarbons and other types of compounds.

The contents of active ingredients in the extracts of dragon's blood obtained under different process conditions are different, and their medicinal effects and uses are all different. For example, CN 101559149 A discloses a kind of dragon's blood and its extracts for the preparation of radiation protection drugs The use of the dragon's blood extract refers to the total phenols of the dragon's blood and the total flavonoids of the dragon's blood; CN 1857570 A discloses a preparation process of the flavonoid extract of the traditional Chinese medicine dragon's blood, and the extract has a strong α- Glucosidase inhibitory activity and insulin secretion-stimulating function can play a role in lowering blood sugar.

In the research and development process of cosmetics, traditional Chinese medicine extracts, as functional additives, are an important material basis for the preparation of cosmetics with special effects. However, due to the imperfect extraction technology of various Chinese herbal medicine additives necessary for the production of these cosmetics, the low technological content and the lack of modern scientific basis for active ingredients, many functional cosmetics of Chinese medicine currently sold in the domestic market have no obvious use effect. . The existing/reported preparation process of Dracaena extract for cosmetics also has many problems such as poor compatibility with cosmetic matrix, poor stability, high allergenicity, low extraction rate or need to be concentrated before adding to cosmetics.

Contents of the invention

The object of the present invention is to provide a dragon's blood extract and its preparation method and application. The method uses polyol or white oil as an organic solvent, and the extraction rate is high, and the prepared dragon's blood extract is less irritating to human skin , low allergenicity, good compatibility with cosmetic matrix, good stability, and has antibacterial, anti-acne, anti-oxidation, anti-aging, whitening effects.

The preparation method of the dragon's blood extract provided by the invention comprises the following steps: using an organic solvent to extract the dragon's blood, and the obtained extract is the dragon's blood extract; the organic solvent is a polyhydric alcohol or white Oil.

In the above preparation method, the dragon's blood can be the raw material drug of dragon's blood; the particle size of the dragon's blood can be 10 mesh to 200 mesh, specifically 40 to 60 mesh, 60 to 100 mesh, 50 to 100 mesh. 80 mesh, 40 mesh, 50 mesh, 60 mesh, 80 mesh or 100 mesh.

In the above-mentioned preparation method, the polyol is preferably used in cosmetics and is less irritating to the skin, such as 1-2-propanediol, 1,3-propanediol, 1,3-butanediol, glycerin, etc. .

In the above-mentioned preparation method, the grade of the white oil can be 15# or 26#. The grades are classified according to the kinematic viscosity at 40°C.

In the above preparation method, the extraction can be immersion extraction or reflux extraction; the specific steps of the immersion extraction are as follows: add the organic solvent to the dragon's blood for soaking, and after filtering, collect the filtrate to obtain The dragon's blood extract.

In the above preparation method, 1-1000 g of the organic solvent is added to every 1 g of the dragon's blood, that is, the solid-liquid ratio can be 1: (1-1000), specifically, 20-100 g of the above-mentioned dragon's blood can be added to each 1 g of the dragon's blood. The organic Solvent, that is, the ratio of solid to liquid can be 1: (20-500), 1: (20-200), 1: (20-100), 1: (20-50), 1: (50-500), 1 :(50～200), 1:(50～100), 1:(100～500), 1:(100～200), 1:(200～500), 1:20, 1:50, 1:100 , 1:200 or 1:500.

In the above preparation method, the extraction temperature can be 25-180°C, specifically 80-150°C, 80-95°C, 80-100°C, 80-110°C, 95-150°C, 95-110°C , 95-100°C, 100-110°C, 100-150°C, 110-150°C, 80°C, 95°C, 100°C, 110°C or 150°C, the time for each extraction can be 10-1440 minutes. 30 to 120 minutes, 30 to 60 minutes, 30 to 90 minutes, 60 to 90 minutes, 60 to 120 minutes, 90 to 120 minutes, 30 minutes, 60 minutes, 90 minutes or 120 minutes.

In the above preparation method, the number of extractions may be 1-3 times, specifically 1-2 times, 2-3 times, 1 time, 2 times or 3 times.

The dragon's blood extract prepared by the above preparation method is also within the protection scope of the present invention. The mass percent content of total phenols in the dragon's blood extract is 0.01-25%.

The above-mentioned dragon's blood extract is used directly as a product with at least one function in the following 1)-8) or in the preparation of a product with at least one function in the following 1)-8), Also within the protection scope of the present invention:

1) Inhibit acne pathogenic bacteria;

2) acne;

3) Scavenge DPPH free radicals;

4) Remove ABTS free radicals;

5) anti-oxidation;

6) anti-aging;

7) inhibit the activity of tyrosinase;

8) Whitening.

In the above-mentioned applications, the product may be cosmetics, and the cosmetics may be in the form of various acceptable preparations such as cream, gel or water.

The dragon's blood extract of the present invention has good compatibility with cosmetic bases, and can be directly added to cosmetics. No matter it is added to water, gel or cream, there is no viscosity reduction or demulsification. In addition to anti-oxidation, Anti-aging effect, also has significant acne and whitening effect.

The present invention has following beneficial effect:

The present invention overcomes the use of organic solvents such as ethanol, methanol, acetone, and ethyl acetate (non-commonly used solvents for cosmetics) to extract dragon's blood in the prior art, and the extraction rate of the extract is not high or needs to be concentrated before being added to cosmetics. Poor matrix compatibility, poor stability, high allergenicity and other issues.

The dragon's blood extract of the present invention has good compatibility with cosmetic bases, and can be directly added to cosmetics. No matter it is added to water, gel or cream, there is no viscosity reduction or demulsification; the product has good stability. , is very stable against harsh conditions such as high temperature, low temperature, and light; the extraction rate is high (reaching more than 95%), and the raw material loss of dragon's blood is small, and the maximum utilization of the raw material of dragon's blood is reached. In addition, in addition to anti-oxidation and anti-aging effects, it also has significant acne-removing and whitening effects.

detailed description

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

Carbopol U20 (Carbopol Ultrez 20 polymer), INCI name: Acrylates/C10-30 AlkylAcrylate Crosspolymer.

MTI is a product purchased from Guangdong Zhongpeng Chemical Co., Ltd., the product name is MTI cosmetic preservative, and its active components are methylisothiazolinone and iodopropynyl butyl carbamate.

NMF-50, the chemical name is trimethylglycine.

HEC (Hydroxyethyl Cellulose), the chemical name is hydroxyethyl cellulose, and the specification is hydroxyethyl cellulose HHR250.

DM100, the chemical name is polydimethylsiloxane.

Mixed alcohol, the chemical name is cetostearyl alcohol.

LGP is a product purchased from Guangdong Zhongpeng Chemical Co., Ltd., the product name is LGP cosmetic preservative, and the active ingredients are DMDM hydantoin, methylisothiazolinone (MIT), iodopropynyl butylcarbamate (IPBC)

Transparent xanthan gum, the chemical name is xanthan gum.

In the following examples, the assay method of total phenolic content in the extract of Dragon's blood is as follows:

a. Preparation of reference substance solution

Precisely weigh 8.82 mg of the standard product of dragon blood B, place it in a 100 mL volumetric flask, add absolute ethanol to constant volume, and shake well to obtain a solution with the content of dragon blood B of 0.0882 mg/ml.

b. Standard curve drawing

Take 0.5, 0.6, 0.75, 1.0, 1.3, and 1.5ml of the reference substance of ascarin B (0.0882mg/ml), place them in 10ml volumetric flasks, add absolute ethanol to volume, and shake well. Absorbance was measured at a selected wavelength of 280 nm with absolute ethanol as a blank. With absorbance as the abscissa and reference substance concentration C (mg/mL) as the ordinate, draw a standard curve.

c. Determination of total phenol content

Precisely take samples to prepare a suitable concentration solution, measure the absorbance at a selected wavelength of 280nm, and calculate the total phenol content according to the established standard curve.

In the following examples, the yield of dragon's blood extract is calculated according to the total phenol content of the dragon's blood extract, the feeding amount and the total amount of the extract after extraction. Calculated as follows:

In formula (1), the meanings and units of each physical quantity are as follows:

C: total phenol content of the extract, mg/mL

V: total volume of extract, mL

M: the mass of total phenols in the raw drug of dragon's blood used in making the extract, g.

Embodiment 1, preparation dragon's blood extract

Crush dragon's blood to 60 mesh, add 1,2-propanediol according to the ratio (mass ratio) of 1:50, soak at 95°C for 1 hour, after the extraction is completed, filter with filter paper, and the obtained filtrate is dragon's blood Extract.

In this example, the ratio of solid to liquid is 1:50, the extraction temperature is 95° C., the extraction time is 1 hour, and the extraction frequency is 1 time. The total phenol content of the obtained extract is 0.91%, and the extraction rate is 95%.

Embodiment 2, preparation dragon's blood extract

Crush dragon's blood to 100 mesh, add 1,3-propanediol according to the ratio (mass ratio) of 1:20, soak at 80°C for 1.5 hours, after the extraction is completed, filter with filter paper, collect the filtrate, and filter Repeat the last operation, and combine all the filtrates to obtain the dragon's blood extract.

In this example, the ratio of solid to liquid is 1:20, the extraction temperature is 80°C, the extraction time is 1.5 hours, and the extraction frequency is 2 times. The total phenol content of the obtained extract is 1.2%, and the extraction rate is 96%.

Embodiment 3, preparation dragon's blood extract

Crush dragon's blood to 40 mesh, add 1,3-butanediol according to the ratio (mass ratio) of 1:100, soak at 100°C for 0.5 hours, after the extraction is completed, filter with filter paper, and the obtained filtrate is dragon Blood extract.

In this example, the ratio of solid to liquid is 1:100, the extraction temperature is 100° C., the extraction time is 0.5 hour, and the extraction frequency is 1 time. The total phenol content of the obtained extract was 0.32%, and the extraction rate was 92%.

Embodiment 4, preparation dragon's blood extract

Crush dragon's blood to 80 mesh, add glycerol according to the ratio (mass ratio) of 1:200, soak at 110°C for 1.5 hours, after the extraction is completed, filter with filter paper to collect the filtrate; repeat the above steps for the obtained filter residue Operation, the combined filtrate is the dragon's blood extract.

In this example, the ratio of solid to liquid is 1:200, the extraction temperature is 110° C., the extraction time is 1.5 hours, and the extraction frequency is 2 times. The total phenol content of the obtained extract is 0.10%, and the extraction rate is 90%.

Embodiment 5, preparation dragon's blood extract

Crush dragon's blood to 50 mesh, add 15# white oil according to the ratio (mass ratio) of 1:500, soak at 150°C for 2 hours, after the extraction is completed, filter with filter paper to collect the filtrate; repeat the obtained filter residue The above operation, the obtained filter residue repeats the above operation again, and all the filtrates are combined to obtain the dragon's blood extract.

In this example, the ratio of solid to liquid is 1:500, the extraction temperature is 150° C., the extraction time is 2 hours, and the extraction frequency is 3 times. The total phenol content of the obtained extract is 0.03%, and the extraction rate is 85%.

Embodiment 6, the application of dragon's blood extract in the preparation of cosmetics

(1) Stability test of dragon's blood extract

Get 100 g of the dragon's blood extract prepared in Example 1 and put them in four transparent sample bottles respectively and place them in different environments, and take four bottles of samples regularly to observe the state simultaneously. The four bottles of samples were placed in a sunny place indoors, in a 38-degree incubator, in a -20-degree refrigerator freezer, and in a cool place at room temperature. After one week, two weeks, and one month, the state changes were observed to be minimal, indicating that the extraction of dragon's blood material has good stability.

Get 100 g of the dragon's blood extract prepared in Example 2 and put them in four transparent sample bottles respectively under different environments, and regularly take four bottles of samples to observe the state simultaneously. The four bottles of samples were placed in a sunny place indoors, in a 38-degree incubator, in a -20-degree refrigerator freezer, and in a cool place at room temperature. After one week, two weeks, and one month, the state changes were observed to be minimal, indicating that the extraction of dragon's blood material has good stability.

Get 100 g of the dragon's blood extract prepared in Example 3 and put them in four transparent sample bottles respectively under different environments, and regularly take four bottles of samples to observe the state simultaneously. The four bottles of samples were placed in a sunny place indoors, in a 38-degree incubator, in a -20-degree refrigerator freezer, and in a cool place at room temperature. After one week, two weeks, and one month, the state changes were observed to be minimal, indicating that the extraction of dragon's blood material has good stability.

Get 100 g of the dragon's blood extract prepared in Example 4 and put them in four transparent sample bottles respectively under different environments, and take four bottles of samples regularly to observe the state simultaneously. The four bottles of samples were placed in a sunny place indoors, in a 38-degree incubator, in a -20-degree refrigerator freezer, and in a cool place at room temperature. After one week, two weeks, and one month, the state changes were observed to be minimal, indicating that the extraction of dragon's blood material has good stability.

Get 100 g of the dragon's blood extract prepared in Example 5 and put them in four transparent sample bottles respectively under different environments, and take four bottles of samples regularly to observe the state simultaneously. The four bottles of samples were placed in a sunny place indoors, in a 38-degree incubator, in a -20-degree refrigerator freezer, and in a cool place at room temperature. After one week, two weeks, and one month, the state changes were observed to be minimal, indicating that the extraction of dragon's blood material has good stability.

(2) In vitro antibacterial test of dragon's blood extract

The acne pathogenic bacteria, Propionibacterium acnes and Staphylococcus aureus, were selected as test strains. Propionibacterium acnes has the ATCC number 10312 and is commercially available from the ATCC. Staphylococcus aureus has ATCC number 10384 and is commercially available from ATCC.

Prepare a bacterial suspension containing approximately 1×10 ⁶ CFU/mL of bacteria. Cut the filter paper into discs with a diameter of 6mm and sterilize them by dry heat at 160°C for 2 hours, and put the filter paper into the antibacterial stock solution (the extract of dragon’s blood prepared in Example 1-Example 5) with sterile tweezers Take out after soaking for 1 hour. Take 0.2mL of bacterial suspension in the corresponding sterile solidified medium, and use a sterile glass triangle to evenly spread it into a flat plate containing bacteria, place the soaked filter paper in the center of the plate, and use it as a blank control, and carry out three Groups of parallel experiments were placed in an anaerobic or biochemical incubator at 37°C for 24 hours.

Take out and observe the inhibition zone, the results are shown in Table 1. The appearance of the antibacterial zone proves that the extract of dragon's blood has a certain inhibitory effect on Propionibacterium acnes and Staphylococcus aureus, and the larger the diameter of the antibacterial zone, the stronger the antibacterial activity.

Table 1. Data table of antibacterial zone of dragon's blood extract

Note: "<6" means no inhibition zone.

(3) Antioxidant efficacy evaluation of dragon's blood extract

1) Determination of DPPH free radical scavenging ability

DPPH is an early synthesized organic free radical, which is often used to evaluate the hydrogen-donating ability of antioxidants. It is very stable in organic solvents, it is purple in color, and has a characteristic absorption peak at 517nm. When it encounters a free radical scavenger When , the lone pair of electrons of DPPH is paired to make it fade, that is, the light absorption value at the maximum absorption wavelength becomes smaller. Therefore, the scavenging effect of the sample on DPPH free radicals can be evaluated by measuring the change of the absorbance value.

Solution to be tested: use sterile water to prepare dilutions containing 0.30% of the stock solution of Dracaena jacquardii stock solution prepared in Examples 1-5. Sterile water was used as the blank control, and 15 μg/mL vitamin C solution was used as the control.

Mix 0.1mL of the solution to be tested with 5.8mL of DPPH solution to form A; mix 0.2mL of absolute ethanol with 5.8mL of DPPH solution to form B; mix 5.8mL of absolute ethanol with 0.2mL of the solution to be tested It is C; after reacting for 30 minutes, measure the absorbance values of A, B, and C respectively at 517 nm.

Calculation method:

Table 3 shows the scavenging rate of 0.3% dragon's blood extract obtained by diluting the dragon's blood extract prepared in different examples and the control to DPPH free radicals.

Table 3. DPPH free radical scavenging rate of dragon's blood extract

2) Determination of ABTS free radical scavenging ability

ABTS is oxidized to green ABTS+ under the action of an appropriate oxidant, and the production of ABTS+ will be inhibited in the presence of antioxidants. The total antioxidant capacity of the sample can be determined and calculated by measuring the absorbance of ABTS at 734nm.

Add 500 μL of the test solution to the test tubes of group A in turn, add 500 μL of ethanol aqueous solution to the test tubes of group B, cover the caps in time after adding the reagents, and vortex to mix. Add 500 μL of ABTS free radical stock solution to test tube A and test tube B in turn, cover the cap in time after adding the reagent, and vortex to mix. After 10 min of reaction, the absorbance was detected at 734 nm. Add 500 μL of the solution to be tested and 500 μL of ethanol aqueous solution to the test tubes of group C in sequence, vortex and mix well, and measure the absorbance at 734 nm.

Calculation method:

Solution to be tested: the Dracaena dracaena stock solutions prepared in Examples 1-5 were prepared with sterile water to a dilution of 0.10%. Sterile water was used as blank control. A 0.001% vitamin C solution was used as a control.

Table 4 shows the scavenging rate of ABTS free radicals of the 0.10% Dracaena extract obtained by diluting the Dracaena extract prepared in different examples and the control.

Table 4. ABTS free radical scavenging rate of dragon's blood extract

From the above experimental data, it can be seen that the extract of dragon's blood has strong antioxidant capacity, can scavenge free radicals, promote cell metabolism, enhance cell vitality, improve the structure and function of the body, and increase the vitality of the body, thereby delaying cell aging and exerting its potential. Anti-aging effect.

(4) Whitening effect of dragon's blood extract

Tyrosinase is an oxidoreductase, which is the main rate-limiting enzyme for melanin synthesis. Its activity determines the amount of melanin formed. Many whitening and freckle products currently on the market achieve whitening effects by inhibiting tyrosinase activity. Therefore, the strength of the inhibitory effect on tyrosinase is the main indicator for evaluating whitening cosmetics.

L-tyrosine generates dopa under the action of tyrosinase, and dopa is further acted by tyrosinase to generate dopaquinone, and finally melanin is generated through a series of biochemical processes. Among them, dopaquinone has a maximum absorption peak at a wavelength of 475nm, and its absorbance is measured per unit time, and the activity of the indirect reaction enzyme is calculated to obtain the inhibition rate of tyrosinase.

The experiment was divided into four groups. In each group, phosphate buffer solution, L-tyrosinase solution and test solution were accurately added according to the dosage of the components in Table 5, mixed evenly, and kept at 37°C for 10 minutes.

Phosphate buffer solution: Weigh 71.63g Na ₂ HPO ₄ 12H ₂ O, fully dissolve it with deionization and set the volume in a 1000mL volumetric flask, and prepare a 0.2mol/L Na ₂ HPO ₄ solution; weigh 31.20g NaH ₂ PO ₄ . 2H ₂ O was fully dissolved in deionized water and settled in a 1000mL volumetric flask to prepare a 0.2mol/L NaH ₂ PO ₄ solution; the two were mixed at a ratio of 1:1 to prepare a phosphate buffer solution with a pH value of 6.8. Tyrosinase solution: Weigh a certain amount of tyrosinase according to the enzyme activity unit of 200U/mL, dissolve it in phosphate buffer, dilute it in a volumetric flask of required volume, and store it in the refrigerator for later use. Among them, when the temperature is 25°C, the pH is 6.5, and the wavelength is 280nm, the decrease of absorbance by 0.001 per minute is an activity unit (U).

Add tyrosinase solution to group A and group C and mix well, react for 10 minutes, measure the absorbance value of four groups at 475nm immediately after the reaction.

Table 5, test solution component dosage table

Calculation method:

Solution to be tested: the stock solution of Dracaena japonicus extract prepared in Examples 1-5 was respectively prepared with sterile water into dilutions containing 1.0% of the stock solution. Sterile water was used as blank control. A 0.1% arbutin solution was used as a control.

Table 6 shows the tyrosinase inhibition rate of the 1.0% dragon's blood extract obtained by diluting the dragon's blood extract prepared in different examples and the control.

Table 6. Inhibition rate of tyrosinase in dragon's blood extract

It can be seen from the above results that within the appropriate concentration range of cosmetics, the extract of dragon's blood exhibits good tyrosinase inhibitory ability, and the extract of dragon's blood is an excellent plant natural whitening ingredient.

(5) Safety evaluation of dragon's blood extract

1) Toxicology testing (skin irritation test)

In this experiment, 5% dragon's blood extract (respectively obtained by diluting the stock solution of dragon's blood extract prepared in Examples 1-5 with sterile water) was used as a test product, and skin Stimulation test, the experimental results are shown in Table 7.

Table 7, skin irritation test data (embodiment 1)

Table 8, skin irritation test data (embodiment 2)

Table 9, skin irritation test data (embodiment 3)

Table 10, skin irritation test data (embodiment 4)

Table 11, skin irritation test data (embodiment 5)

The experimental data showed that the 5% dragon's blood extract had no irritating reaction, passed the skin irritation test smoothly, and met the requirements.

2) Human body safety testing (human skin patch test)

This experiment uses 5% concentration of dragon's blood extract (respectively obtained by diluting the stock solution of dragon's blood extract prepared in Examples 1-5 with sterile water) as a test product, according to the requirements of "Hygienic Standards for Cosmetics" 2007 edition In the skin-occlusive patch test, the number of participants in the experiment was 30, and the test data are shown in Table 8.

Table 12, human skin patch test data (embodiment 1)

Table 13, human skin patch test data (embodiment 2)

Table 14, human skin patch test data (embodiment 3)

Table 15, human skin patch test data (embodiment 4)

Table 16, human skin patch test data (embodiment 5)

Experimental data show that the 5% dragon's blood extract has no adverse reactions on human skin, and successfully passed the human skin patch test, meeting the requirements.

Therefore, through toxicology testing and human safety testing, it has been proved that the extract of dragon's blood has no stimulation or any adverse reaction to the human body when used in an appropriate concentration range, and it is safe and reliable to add the extract of dragon's blood to cosmetics.

Embodiment 7, the preparation of dragon's blood extract cosmetic (aqueous form)

1. Formula

Formula 1: Dragon's blood extract (experiment 1) 1.0%, deionized water 87.5%, NMF-50 5.0%, sorbitol 2.0%, allantoin 0.1%, glycerol 4.0%, transparent xanthan gum 0.1% , LGP 0.3%.

Formula 2: Dragon's blood extract (experiment 2) 1.5%, deionized water 87%, NMF-50 5.0%, sorbitol 2.0%, allantoin 0.1%, glycerol 4.0%, transparent xanthan gum 0.1% , LGP 0.3%.

Formula 3: Dragon's Blood Extract (Experiment 3) 2%, Deionized Water 87%, NMF-50 4.5%, Sorbitol 2.0%, Allantoin 0.1%, Glycerol 4.0%, Transparent Xanthan Gum 0.1% , LGP 0.3%.

Formula 4: Dragon's Blood Extract (Experiment 4) 2.5%, Deionized Water 87%, NMF-50 4.5%, Sorbitol 2.0%, Allantoin 0.1%, Glycerol 3.5%, Transparent Xanthan Gum 0.1% , LGP 0.3%.

Formula 5: Dragon's Blood Extract (Experiment 5) 3%, Deionized Water 87%, NMF-50 4.5%, Sorbitol 1.5%, Allantoin 0.1%, Glycerol 3.5%, Transparent Xanthan Gum 0.1% , LGP 0.3%.

2. Preparation method

Table 16. Raw material formula of water-like dragon's blood extract cosmetics

As shown in Table 16, weigh all the ingredients of phase A in proportion, mix them, stir and heat to 95°C for 30 minutes, then cool down to 60°C and add phase B, stir evenly, and fill.

Embodiment 8, preparation of dragon's blood extract cosmetic (gel)

1. Formula

Formula 1: Dragon's Blood Extract (Experiment 1) 1.0%, Deionized Water 88.5%, Sorbitol 2%, Glycerol 4.0%, 1,3-Butanediol 4.0%, Kappa U20 0.3%, MTI0. 15%, NAON 0.05%.

Formula 2: Dragon's Blood Extract (Experiment 2) 1.5%, Deionized Water 88.2%, Sorbitol 2%, Glycerol 3.7%, 1,3-Butanediol 4.0%, Kappa U20 0.3%, MTI0. 2%, NAON 0.1%.

Formula 3: Dragon's Blood Extract (Experiment 3) 2%, Deionized Water 88.2%, Sorbitol 2%, Glycerol 3.7%, 1,3-Butanediol 3.5%, Kappa U20 0.3%, MTI0. 2%, NAON 0.1%.

Formula 4: Dragon's Blood Extract (Experiment 4) 2.5%, Deionized Water 88%, Sorbitol 2%, Glycerol 3.5%, 1,3-Butanediol 3.3%, Kappa U20 0.3%, MTI0. 2%, NAON 0.2%.

Formula 5: Dragon's Blood Extract (Experiment 5) 3%, Deionized Water 88%, Sorbitol 2%, Glycerol 3.3%, 1,3-Butanediol 3.0%, Kappa U20 0.3%, MTI0. 2%, NAON 0.2%.

2. Preparation method

Table 17. Raw material formula of gel-like dragon's blood extract cosmetics

As shown in Table 17, weigh all the ingredients of phase A in proportion, mix them, stir, heat to 95°C and keep it warm for 30 minutes, then cool down to 60°C and add phase B, stir well, add phase C, stir well, vacuumize, and fill That's it.

Embodiment 9, the preparation of dragon's blood extract cosmetic (cream)

1. Formula

Formula 1: Dragon's blood extract (experiment 1) 1.0%, deionized water 73.6%, glycerin 5.0%, NMF-50 5.0%, HEC 0.1%, sorbitol 2.0%, D-panthenol 1.0%, monoglycerin 2.0% ester, 7.0% white oil, 2.0% DM100, 1.0% mixed alcohol, 0.3% LGP.

Formula 2: Dragon's blood extract (experiment 2) 1.5%, deionized water 73%, glycerol 5.5%, NMF-50 4.5%, HEC 0.2%, sorbitol 2.0%, D-panthenol 1.0%, mono Glyceride 2.0%, white oil 7.0%, DM100 2.0%, mixed alcohol 1.0%, LGP 0.3%.

Formula 3: Dragon's blood extract (experiment 3) 2%, deionized water 73%, glycerol 5.5%, NMF-50 4.0%, HEC 0.2%, sorbitol 2.0%, D-panthenol 1.0%, mono Glyceride 2.0%, white oil 7.0%, DM100 2.0%, mixed alcohol 1.0%, LGP 0.3%.

Formula 4: Dragon's blood extract (experiment 4) 2.5%, deionized water 73%, glycerol 5.0%, NMF-50 4.0%, HEC 0.2%, sorbitol 2.0%, D-panthenol 1.0%, mono Glyceride 2.0%, white oil 7.0%, DM100 2.0%, mixed alcohol 1.0%, LGP 0.3%.

Formula 5: Dragon's blood extract (experiment 5) 3.0%, deionized water 73%, glycerol 4.5%, NMF-50 4.0%, HEC 0.2%, sorbitol 2.0%, D-panthenol 1.0%, mono Glyceride 2.0%, white oil 7.0%, DM100 2.0%, mixed alcohol 1.0%, LGP 0.3%.

2. Preparation method

Table 18. Raw material formula of cream-like dragon's blood extract cosmetics

As shown in Table 18, weigh all the ingredients of phase A and phase B in proportion, mix them, stir and heat to 95°C for 30 minutes, homogenize for 30 minutes, stir and cool down to 60°C, add phase C, stir evenly, and vacuum , can be filled.

Embodiment 10, clinical trial

(1) For the cosmetics prepared in the above examples, 192 volunteers aged 18-25 were selected, randomly divided into 16 groups, 12 people in each group, and tried continuously for 30 days (each person only used the corresponding embodiment or comparative example cosmetics, Do not use other acne-removing products), comparative analysis and evaluation of the acne-removing effect of the cosmetics prepared in each of the above examples, the results are shown in Table 19.

Test area: Acne on the face.

Evaluation criteria: markedly effective, effective, and ineffective. Among them, the basic elimination of acne is judged to be markedly effective; the partial elimination of acne is judged to be effective; the acne is not reduced or the reduction is not obvious, and it is judged to be invalid.

Comparative example 1: A commercially available acne-removing cosmetic of a certain brand.

Table 19, the acne-removing effect of the dried dragon's blood extract cosmetics prepared in each embodiment

group efficient markedly effective invalid Embodiment 7 (recipe 1) 3 8 1 Embodiment 7 (recipe 2) 3 9 0 Embodiment 7 (recipe 3) 3 8 1 Embodiment 7 (recipe 4) 3 6 3 Embodiment 7 (recipe 5) 3 5 4 Embodiment 8 (recipe 1) 6 6 0 Embodiment 8 (recipe 2) 5 5 2 Embodiment 8 (recipe 3) 5 5 2 Embodiment 8 (recipe 4) 5 4 3 Embodiment 8 (recipe 5) 4 4 4 Embodiment 9 (recipe 1) 4 7 1 Embodiment 9 (recipe 2) 4 7 1 Embodiment 9 (recipe 3) 4 6 2 Embodiment 9 (recipe 4) 4 6 2 Embodiment 9 (recipe 5) 3 5 4 Comparative example 1 2 4 6

(2) For the cosmetics prepared in the above-mentioned examples, 160 persons aged 25-55 were selected, randomly divided into 16 groups, 10 people in each group, and tried for 30 days in a row (each person only used the corresponding embodiment or comparative example cosmetics, No longer using other whitening and anti-oxidation products), comparative analysis and evaluation of the whitening and anti-oxidation effects of the cosmetics prepared in each of the above examples. Among them, the whitening and anti-oxidation effects refer to the improvement of dark skin, pigmentation, rough skin, etc., and the results are shown in Table 20.

Test area: facial skin.

Evaluation criteria: The degree of skin improvement is identified through the patient's self-reported feelings and computer comparison with the skin texture map. Effective: brighten skin tone, lighten pigmentation spots, and improve skin fineness. Invalid: Skin color, pigmentation and fineness have no change from before the test.

Comparative Example 2: Whitening and antioxidant cosmetics of a certain brand on the market.

Table 20, the whitening and anti-oxidation effects of the dragon's blood extract cosmetics prepared in each embodiment

group efficient invalid Embodiment 7 (recipe 1) 10 0 Embodiment 7 (recipe 2) 9 1 Embodiment 7 (recipe 3) 9 1 Embodiment 7 (recipe 4) 8 2 Embodiment 7 (recipe 5) 7 3 Embodiment 8 (recipe 1) 10 0 Embodiment 8 (recipe 2) 9 1 Embodiment 8 (recipe 3) 9 1 Embodiment 8 (recipe 4) 9 1 Embodiment 8 (recipe 5) 8 2 Embodiment 9 (recipe 1) 10 0 Embodiment 9 (recipe 2) 10 0 Embodiment 9 (recipe 3) 9 1 Embodiment 9 (recipe 4) 8 2 Embodiment 9 (recipe 5) 7 3 Comparative example 2 4 6

Claims (10)

1. A preparation method for a dragon's blood extract, comprising the steps of: adopting an organic solvent to extract the dragon's blood, and the obtained extract is the described dragon's blood extract; the organic solvent is polyhydric alcohol or white oil . 2. The preparation method according to claim 1, characterized in that: the particle size of the dragon's blood is 10 mesh to 200 mesh. 3. The preparation method according to claim 1 or 2, characterized in that: the polyhydric alcohol is any one of 1-2-propanediol, 1,3-propanediol, 1,3-butanediol and glycerol . 4. The preparation method according to any one of claims 1-3, characterized in that: the grade of the white oil is 15# or 26#. 5. The preparation method according to any one of claims 1-4, characterized in that: the extraction is immersion extraction or reflux extraction. 6. The preparation method according to any one of claims 1-5, characterized in that 1-1000 g of the organic solvent is added to 1 g of the dragon's blood. 7. The preparation method according to any one of claims 1-6, characterized in that: the temperature of the extraction is 25-180° C., and the time of each extraction is 10-1440 minutes. 8. The preparation method according to any one of claims 1-7, characterized in that: the number of extractions is 1-3 times. 9. The dragon's blood extract prepared by the preparation method described in any one of claims 1-8. 10. The application of the dragon's blood extract described in claim 9 directly as a cosmetic having at least one function in the following 1)-8) or in the preparation of at least one of the following 1)-8) Functional Cosmetic Applications: 1) Inhibit acne pathogenic bacteria; 2) acne; 3) Scavenge DPPH free radicals; 4) Scavenge ABTS free radicals; 5) anti-oxidation; 6) anti-aging; 7) inhibit the activity of tyrosinase; 8) Whitening.