CN106501132B - A kind of anti-fouling material anti-biofouling performance test method - Google Patents
A kind of anti-fouling material anti-biofouling performance test method Download PDFInfo
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- 239000000463 material Substances 0.000 title claims abstract description 120
- 230000003373 anti-fouling effect Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000011056 performance test Methods 0.000 title claims abstract description 12
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 77
- 239000000227 bioadhesive Substances 0.000 claims abstract description 74
- 238000012360 testing method Methods 0.000 claims abstract description 65
- 239000011230 binding agent Substances 0.000 claims abstract description 64
- 239000007864 aqueous solution Substances 0.000 claims abstract description 44
- 238000011156 evaluation Methods 0.000 claims abstract description 14
- 239000011248 coating agent Substances 0.000 claims abstract description 8
- 238000000576 coating method Methods 0.000 claims abstract description 8
- 230000008569 process Effects 0.000 claims abstract description 8
- 238000002474 experimental method Methods 0.000 claims abstract description 6
- 239000012496 blank sample Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
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- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000001179 sorption measurement Methods 0.000 claims description 5
- 238000006467 substitution reaction Methods 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000000502 dialysis Methods 0.000 claims description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 238000005096 rolling process Methods 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000000017 hydrogel Substances 0.000 abstract description 2
- 239000011347 resin Substances 0.000 abstract description 2
- 229920005989 resin Polymers 0.000 abstract description 2
- 241000195493 Cryptophyta Species 0.000 description 51
- 239000000523 sample Substances 0.000 description 6
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- 238000012216 screening Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 3
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- 239000002519 antifouling agent Substances 0.000 description 2
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- 239000002210 silicon-based material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 238000004140 cleaning Methods 0.000 description 1
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- 229910052802 copper Inorganic materials 0.000 description 1
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- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 description 1
- KRFJLUBVMFXRPN-UHFFFAOYSA-N cuprous oxide Chemical compound [O-2].[Cu+].[Cu+] KRFJLUBVMFXRPN-UHFFFAOYSA-N 0.000 description 1
- 229940112669 cuprous oxide Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 238000010327 methods by industry Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000010071 organism adhesion Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N13/00—Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects
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Abstract
The invention belongs to anti-fouling material technical field of performance test, more particularly to a kind of anti-fouling material anti-biofouling performance test method, pass through the contact angle and roll angle of experiment test 6-10 group various concentration bio-adhesive agent aqueous solution, bio-adhesive agent is calculated in the contact angle and roll angle on detected materials surface in linear fit respectively, then it is compared respectively with the contact angle of blank sample and roll angle, calculate the antibiont binder sticking coefficient of detected materials, and with antibiont binder sticking coefficient as a comparison with evaluation bio-adhesive agent detected materials surface attachment characteristic parameter；Its process flow is simple, principle is reliable, it is repeatable strong, the test process time is short, environmental protection and energy saving, and economic cost is low, strong operability, test result accuracy is high, applied to the nonpolluting coating of low-surface-energy, surface micro-structure, hydrogel and pollution release type and the Fast Evaluation of resin, has good promotion and application prospect.
Description
Technical field:
The invention belongs to anti-fouling material technical field of performance test, and in particular to a kind of anti-fouling material anti-biofouling performance
Test method is tested and is evaluated ship and ocean engineering in marine environment using bio-adhesive agent and set under water in room conditions
The anti-pollution for applying surface preventing material has effects that quickly screening and accurate evaluation.
Background technique:
Marine biofouling is adhesion marine organisms in ship and ocean engineering facility surface adhesion and causes damages
Phenomenon, marine biofouling increase ship resistance, cause maritime internal diameter of the pipeline to reduce and then block, cause Coastal Power Plant
The heat transfer efficiency of cooling equipment reduces, and can also make fishery cultivating net cage obstruction that the decline of nutrition circulation efficiency be caused to cause the underproduction；It is anti-
Except biodeterioration frequently with measure be to construct one layer of protective materials in open environments such as ship housing outer surfaces, including brush anti-
Dirty coating and plating copper protective layer, protective materials generate inhibiting effect to the attachment and growth of fouling organism, realize antifouling purpose；
The service life of anti-fouling material usually only has several years service lifes, and the phase effect of cuprous oxide anti-fouling paint in the prior art is general
It is 3 years, antifouling phase effect is for ship shortlyer, it is meant that dock repair interval is too short, reduces in boat rate, restricted voyage mileage；
For the facility of strand, then frequent cleaning work is needed, so extending anti-fouling material phase effect is the unremitting of marine anti-pollution field
It pursues, is based on this, screening and evaluation to anti-fouling material are essential links.Currently, the survey of anti-fouling material anti-pollution
Examination is mainly using real extra large hanging plate method, according to the antifouling template shallow sea soak test method of GB5370-2007 and GB7789-2007
The period that anti-fouling paint anti-pollution dynamic test method is tested is usually 1-3, slows down the screening of anti-fouling material
Speed limits the development speed of anti-fouling material, and is influenced by marine environment, the test knot of different location and Various Seasonal
There are great differences for fruit.A kind of side of fast simple screening and assessment marine antifoulant disclosed in Chinese patent 201310725873.4
Gelling agent is added in bacterial liquid culture medium using the marine bacteria for being isolated from ocean as biological subject and dissolves by heating, is cold by method
But after, test-compound is dispersed in gel solution, then will contain test-compound gel solution even spread glass
Gel test board is made on plate, gel test board is placed in the seawater of inoculation biological subject, after continuous culture, gel is tested
Plate takes out, elutes, and constant volume tests OD value (OD value) or absorbance value in volumetric flask, and OD value or absorbance value are converted
Screening at bacteriostasis rate, by comparing the anti-pollution of the size evaluation test-compound of bacteriostasis rate, for anti-fouling agent；It is Chinese special
A kind of device that simulation flowing Seawater influences nonpolluting coating performance disclosed in benefit 201120484136.6, including sink, connection
Sink outlet end water pump, be connected to pump outlet steady-flow tank, be connected in steady-flow tank in parallel outlet end and sink
Two sample testing circuits, sheet specimens installing pipe and at least two sections of tubulose sample installing pipes between input end, sink it is upper
Side is equipped with electric light, and sample testing circuit includes inlet tube and same outlet tube, is equipped with sample cock on outlet tube, for containing antifouling
The performance test of the nonpolluting coating of agent；201010531314.6 a kind of flow channel type marine organism adhesion testing device, box type
Upside right-to-left is successively formed respectively with water inlet, aperture stainless steel plate, honeycomb polycarbonate plate, no in the expansion chamber of structure
Rust steel sieve plate and exhaust valve, expansion chamber left end are laterally formed with test chamber, and test chamber left end is formed with differential pressure transmitter on side, test
Vacuum pump is equipped in chamber, the left-hand end of test chamber is equipped with sink, and the top of sink is formed with light source, and bottom of gullet introduction pipe passes through
The control of multiterminal valve is connected to water inlet, is successively formed respectively with variable frequency pump, flowmeter and pressure transmitter in the pipeline of connection,
The ability of fouling organism is removed for evaluating material under external force.Therefore, the indoor Fast Evaluation side of marine anti-pollution material is established
Method helps speed up the development rate of novel antifouling material, improves the development efficiency of new product, is efficient long-acting anti-fouling material
Invention provides technical support, a kind of anti-fouling material anti-biofouling performance test method is researched and developed, using bio-adhesive agent in solid
The touching act test bio-adhesive agent on surface detected materials surface wellability, it is micro- for evaluating low-surface-energy type, surface
The anti-pollution of the nonpolluting coating of structural type and pollution release type achievees the purpose that Fast Evaluation detected materials fouling resistance performance.
Summary of the invention:
It is an object of the invention to overcome disadvantage of the existing technology, a kind of anti-fouling material anti-biofouling of R & D design
Performance test methods utilize the anti-biofouling of the direct Fast Evaluation anti-fouling material of fouling organism binder indoors in environment
Energy.
To achieve the goals above, the technical process of anti-fouling material anti-biofouling performance test method of the present invention
Including preparing bio-adhesive agent, test contact angle, test roll angle, calculating anti-pollution five steps of anti-sticking coefficient and evaluation
It is rapid:
(1), bio-adhesive agent is prepared: in room conditions by fouling organism diatom body pure culture to 105-106A/mL, takes
500mL diatom body is placed in centrifuge tube remove salt therein with aseptic distillation water washing diatom body after, be placed in a centrifuge with
The speed of 12000r/min is centrifuged 10min, removes supernatant, then be centrifuged after being rinsed 1-3 times with sterile purified water and remove supernatant
Liquid；Diatom body 5-30min is extracted with the ethanol water that 20mL mass percent concentration is 80-95% again, after centrifugation in removal
Clear liquid repeats extraction, centrifugation and removes supernatant 3 times, obtains diatom body sediment, diatom body sediment is suspended in 20mL and is gone out
In bacterium distilled water, it is heated to 70-95 DEG C, flooding diatom body sediment 0.5-4h is distilled with 20mL, supernatant is collected after centrifugation,
Dialyse supernatant 24-48h in distilled water, replaces primary distilled water every 6h, and the supernatant low-temperature frozen after dialysis is real, obtains
Solid-state supernatant is placed in vacuum freeze drier and is freeze-dried 24-96h removing moisture content, it is glutinous to obtain biology by solid-state supernatant
Attached dose, freeze spare, the preparation of completion bio-adhesive agent；
(2), it tests and calculates contact angle: bio-adhesive agent being taken to be configured to the life of 6 to 10 parts of 0.1-1mg/mL respectively with water
Object binder aqueous solution rises the concentration of bio-adhesive agent aqueous solution successively in gradient, uses video optics Contact-angle measurement
Instrument tests 6 parts of bio-adhesive agent aqueous solutions in the contact angle on No.1 detected materials surface, every part of bio-adhesive agent aqueous solution respectively
5 parallel contact angle numbers are tested, are averaged, by the concentration conversion of bio-adhesive agent aqueous solution at mass percent concentration,
Using the mass percent concentration of bio-adhesive agent as abscissa, the contact angle with bio-adhesive agent on No.1 detected materials surface is
Ordinate, the contact angle corresponding to the mass percent concentration of bio-adhesive agent aqueous solution carry out linear fit, are connect
The mass percent concentration c of bio-adhesive agent is 100 substitution contact angle fit equations, calculates biology by feeler fit equation
Contact angle θ of the binder on No.1 detected materials surface；
(3), it tests and calculates roll angle: bio-adhesive agent being taken to be configured to the life of 6-10 parts of 0.1-1mg/mL respectively with water again
Object binder aqueous solution is risen the concentration of bio-adhesive agent aqueous solution successively in gradient, is surveyed respectively using inclined-plane with a scale
6 parts of bio-adhesive agent aqueous solutions are tried in the roll angle on No.1 detected materials surface, every part of bio-adhesive agent aqueous solution is tested 5 and put down
Row roll angle degree calculates average value, glutinous with biology by the concentration conversion of bio-adhesive agent aqueous solution at mass percent concentration
Attached dose of mass percent concentration be abscissa, using bio-adhesive agent No.1 detected materials surface roll angle as ordinate,
The roll angle corresponding to the mass percent concentration of bio-adhesive agent aqueous solution carries out linear fit, obtains roll angle fitting
The mass percent concentration c of bio-adhesive agent is 100 substitution roll angle fit equations, calculates bio-adhesive agent and exist by equation
The roll angle on No.1 detected materials surface
(4), it calculates anti-sticking coefficient: using the blank sample of glass slide or blank coating as compareing test material,
It is tested respectively using step (2) and step (3) and calculates bio-adhesive agent in the contact angle θ on control test material surfaceXWith
Roll angleBy bio-adhesive agent in No.1 detected materials and the contact angle θ and θ that compare test material surfaceXSubstitute into formula:Obtain contact angle coefficient of correlation f, by bio-adhesive agent No.1 detected materials with compare test material table
The roll angle in faceWithSubstitute into formula:Obtain roll angle coefficient of correlation fX, use f-fXNo.1 is calculated to wait for
Measure and monitor the growth of standing timber the antibiont binder sticking coefficient Aad of material；Or by bio-adhesive agent No.1 detected materials with compare test material
The contact angle θ and θ on surfaceXAnd bio-adhesive agent is in No.1 detected materials and the roll angle for compareing test material surfaceWith
Substitute into formula:The antibiont binder sticking coefficient of No.1 detected materials is calculated
Aad；
(5), it evaluates anti-pollution: experiment being compared to multiple detected materials simultaneously according to step (2), (3) and (4),
Obtain the antibiont binder sticking coefficient of each detected materials, Aad numerical value is smaller, and bio-adhesive agent is easier in detected materials
Adsorption shows that the antibiont binder Adhesion property of detected materials is weaker, and Aad numerical value is bigger, and bio-adhesive agent is not allowed more
Easily in detected materials adsorption, show that the antibiont binder Adhesion property of detected materials is stronger.
Compared with prior art, the present invention passing through the contact of experiment test 6-10 group various concentration bio-adhesive agent aqueous solution
Angle and roll angle, bio-adhesive agent is calculated in the contact angle and roll angle on detected materials surface, then in linear fit respectively
It is compared respectively with the contact angle of blank sample and roll angle, calculates the antibiont binder sticking coefficient of detected materials, and with
Antibiont binder sticking coefficient as a comparison with evaluation bio-adhesive agent detected materials surface attachment characteristic parameter, solve
The problem of bio-adhesive agent contact angle and roll angle in marine environment can not directly be tested, does not need long-term real sea experiment and indoor
Fouling organism is cultivated, mechanism is stained based on bio-adhesive agent and detected materials interface cohesion, utilizes the bio-adhesive agent of preservation
The anti-pollution for realizing quickly detected materials different with effective evaluation, close to the mechanism of the biodeterioration of nature；Its technique
Process is simple, and principle is reliable, and repeatability is strong, and the test process time is short, and environmental protection and energy saving, economic cost is low, strong operability, surveys
Test result accuracy is high, the nonpolluting coating and resin applied to low-surface-energy, surface micro-structure, hydrogel and pollution release type
Fast Evaluation has good promotion and application prospect.
Detailed description of the invention:
Fig. 1 be the present embodiments relate to boat-shaped algae binder surface micro-structure organosilicon material H2 and control test
Schematic diagram on material.
Fig. 2 be the present embodiments relate to boat-shaped algae binder mass percent concentration and contact angle homologous thread illustrate
Figure.
Fig. 3 be the present embodiments relate to boat-shaped algae binder mass percent concentration and roll angle homologous thread illustrate
Figure.
Specific embodiment:
The present invention is described further by way of example and in conjunction with the accompanying drawings.
Embodiment 1:
The process engineering for the anti-fouling material anti-biofouling performance test method that the present embodiment is related to includes that preparation biology is glutinous
Attached dose, test and calculate contact angle, test and calculate roll angle, calculate anti-sticking coefficient and evaluation five steps of anti-pollution:
(1), bio-adhesive agent is prepared: in room conditions by boat-shaped algae pure culture to 106Centrifugation is transferred to after a/mL
Pipe takes 500mL boat-shaped algae to remove salt therein with aseptic distillation water washing, is placed in a centrifuge the speed with 12000r/min
It is centrifuged 10min, supernatant is removed, then be centrifuged and remove supernatant after being rinsed 1 time with sterile purified water, with 20mL mass percent
The ethanol water that concentration is 90% extracts boat-shaped algae 10min, and supernatant is removed after centrifugation, and repetition extracts, is centrifuged and goes
Except supernatant 3 times, boat-shaped algae sediment is obtained, boat-shaped algae sediment is suspended in 20mL sterile purified water, is heated to 90 DEG C,
Flooding 1h is distilled with 20mL, supernatant is collected after centrifugation, supernatant is dialysed in distilled water for 24 hours, replaces primary steam every 6h
Supernatant after dialysis is freezed for 24 hours under the conditions of -25 DEG C, obtains solid-state supernatant, be then placed in solid-state supernatant by distilled water
It is freeze-dried 48h in vacuum freeze drier and removes moisture content, obtains boat-shaped algae binder, wherein the quality group of polysaccharide and protein
It is divided into 70 and 30, freezes spare, the preparation of completion boat-shaped algae binder；
(2), it tests and calculates contact angle: 6 parts of 3mg boat-shaped algae binders being taken to be distinguished in 6 5mL centrifuge tubes with water respectively
The boat-shaped algae that concentration is 1mg/mL, 0.8mg/mL, 0.6mg/mL, 0.4mg/mL, 0.2mg/mL and 0.1mg/mL is configured to stick
Agent aqueous solution, test 6 parts of boat-shaped algae binder aqueous solutions respectively using video optics contact angle measurement has in surface micro-structure
The concentration conversion of boat-shaped algae binder aqueous solution is mass percent concentration, with boat-shaped by the contact angle on the surface machine silicon materials H2
The mass percent concentration of algae binder is abscissa, with boat-shaped algae binder on the surface surface micro-structure organosilicon material H2
Contact angle is ordinate, and the contact angle corresponding to the mass percent concentration of boat-shaped algae binder aqueous solution carries out Linear Quasi
It closes, obtains contact angle fitting formula: y1=-0.386x1+ 142.7, it is 100 by the mass percent concentration c of boat-shaped algae binder
Contact angle fitting formula is substituted into, calculating contact angle θ of the boat-shaped algae binder on the surface surface micro-structure organosilicon material H2 is
104.1°；
Wherein contact angle test process are as follows: open video optics contact angle measurement, boat-shaped algae binder aqueous solution is filled
Enter in syringe, even curface micro-structure organosilicon material H2 is placed on sample stage, the position for adjusting sample stage makes surface
Micro-structure organosilicon material H2 is in the coverage of camera, water-soluble from the syringe needle 10 μ L boat-shaped algae binders that drip
Liquid records a contact angle to the surface surface micro-structure organosilicon material H2, at interval of 10s, and record total time is 3min, and every part
Boat-shaped algae binder aqueous solution tests 5 parallel contact angle numbers, and the contact angle degree after taking 3min clock calculates average value, according to
Boat-shaped algae binder concentration of aqueous solution successively tests the contact angle of 6 parts of boat-shaped algae binder aqueous solutions from low to high；
(3), it tests and calculates roll angle: 6 parts of 3mg boat-shaped algae binders being taken to be distinguished in 6 5mL centrifuge tubes with water respectively
The boat-shaped algae that concentration is 1mg/mL, 0.8mg/mL, 0.6mg/mL, 0.4mg/mL, 0.2mg/mL and 0.1mg/mL is configured to stick
Agent aqueous solution tests 6 parts of boat-shaped algae binder aqueous solutions in surface micro-structure organosilicon material using inclined-plane with a scale respectively
The concentration conversion of boat-shaped algae binder aqueous solution is mass percent concentration, with boat-shaped algae binder by the roll angle on the surface H2
Mass percent concentration be abscissa, the roll angle with boat-shaped algae binder on the surface surface micro-structure organosilicon material H2 is
Ordinate, the roll angle corresponding to the mass percent concentration of boat-shaped algae binder aqueous solution carry out linear fit, obtain
Roll angle fitting formula: y2=-0.051x2+ 60.69, it is that 100 substitutions roll by the mass percent concentration c of boat-shaped algae binder
Angle fitting formula calculates bio-adhesive agent in the roll angle of surface micro-structure organosilicon material H2It is 55.6 °；
Roll angle test process therein are as follows: boat-shaped algae binder aqueous solution is fitted into syringe, by even curface
Micro-structure organosilicon material H2 is placed on inclined-plane with a scale, from syringe needle drip 40 μ L boat-shaped algae binder aqueous solutions to
The surface surface micro-structure organosilicon material H2, slow spin canting record the roll angle of boat-shaped algae binder aqueous solution, every part of boat
Shape algae binder aqueous solution test 5 parallel rolling angle numbers, calculate average value, according to boat-shaped algae binder concentration of aqueous solution from
The low to high roll angle for successively testing 6 parts of boat-shaped algae binder aqueous solutions；
(4), it calculates anti-sticking coefficient: using smooth blank sample as control test material, using step (2) and step
(3) it tests respectively and calculates boat-shaped algae binder in the contact angle and roll angle on control test material surface, boat-shaped algae binder
Contact angle fit equation on control test material surface are as follows: y3=-0.171x3+ 107.7, boat-shaped algae binder is calculated and exists
Compare the contact angle θ on test material surfaceXIt is 90.6 °；Roll angle fit equation of the boat-shaped algae binder on control material surface
Are as follows: y4=0.159x4+ 48.78, roll angle of the boat-shaped algae binder on control test material surface, which is calculated, isFor
64.7°；By boat-shaped algae binder in surface micro-structure organosilicon material H2 and the contact angle θ and θ that compare test material surfaceXGeneration
Enter formula 1:Obtaining contact angle coefficient of correlation f is 1.11, and bio-adhesive agent is organic in surface micro-structure
Silicon materials H2 and the roll angle for compareing test material surfaceWithSubstitute into formula 2:Obtain roll angle comparison system
Number fXIt is 0.91, uses f-fXThe antibiont binder sticking coefficient Aad that surface micro-structure organosilicon material H2 is calculated is
0.20；Or by bio-adhesive agent in surface micro-structure organosilicon material H2 and the contact angle θ and θ that compare test material surfaceX
And bio-adhesive agent is in surface micro-structure organosilicon material H2 and the roll angle for compareing test material surfaceWithIt substitutes into public
Formula 3:The antibiont binder that surface micro-structure organosilicon material H2 is calculated, which sticks, is
Number Aad is 0.20；
(5), it evaluates anti-pollution: experiment being compared to multiple detected materials simultaneously according to step (2), (3) and (4),
The anti-boat-shaped algae binder sticking coefficient for obtaining each detected materials illustrates boat when the Aad numerical value of certain detected materials is less than 0.20
Shape algae binder is easier in the detected materials adsorption, shows the anti-boat-shaped algae binder Adhesion property ratio of the detected materials
The anti-boat-shaped algae binder Adhesion property of surface micro-structure organosilicon material H2 is weak, when the Aad numerical value of certain detected materials is greater than
0.20, illustrate that boat-shaped algae binder is less susceptible to show in the detected materials adsorption that the anti-boat-shaped algae of the detected materials is glutinous
The anti-boat-shaped algae binder Adhesion property of attached dose of Adhesion property specific surface micro-structure organosilicon material H2 is strong.
Claims (1)
1. a kind of anti-fouling material anti-biofouling performance test method, it is characterised in that technical process includes preparing bio-adhesive
Agent, test simultaneously calculate contact angle, test and calculate roll angle, calculate anti-sticking coefficient and evaluation five steps of anti-pollution:
(1), bio-adhesive agent is prepared: in room conditions by fouling organism diatom body pure culture to 105-106A/mL, takes
500mL diatom body is placed in centrifuge tube remove salt therein with aseptic distillation water washing diatom body after, be placed in a centrifuge with
The speed of 12000r/min is centrifuged 10min, removes supernatant, then be centrifuged after being rinsed 1-3 times with sterile purified water and remove supernatant
Liquid；Diatom body 5-30min is extracted with the ethanol water that 20mL mass percent concentration is 80-95% again, after centrifugation in removal
Clear liquid repeats extraction, centrifugation and removes supernatant 3 times, obtains diatom body sediment, diatom body sediment is suspended in 20mL and is gone out
In bacterium distilled water, it is heated to 70-95 DEG C, with 20mL aseptic distillation flooding diatom body sediment 0.5-4h, is collected after centrifugation
Clear liquid, dialyse supernatant 24-48h in sterile purified water, a sterile purified water is replaced every 6h, by the supernatant after dialysis
Low-temperature frozen is real, obtains solid-state supernatant, and solid-state supernatant is placed in vacuum freeze drier and is freeze-dried 24-96h removing water
Part, bio-adhesive agent is obtained, spare, the preparation of completion bio-adhesive agent is freezed；
(2), test and calculate contact angle: the biology for taking bio-adhesive agent to be configured to 6 to 10 parts of 0.1-1mg/mL respectively with water is glutinous
Attached dose of aqueous solution rises the concentration of bio-adhesive agent aqueous solution successively in gradient, uses video optics contact angle measurement point
Not Ce Shi contact angle of 6 to 10 parts of bio-adhesive agent aqueous solutions on No.1 detected materials surface, every part of bio-adhesive agent aqueous solution
5 parallel contact angle numbers are tested, are averaged, by the concentration conversion of bio-adhesive agent aqueous solution at mass percent concentration,
Using the mass percent concentration of bio-adhesive agent as abscissa, the contact angle with bio-adhesive agent on No.1 detected materials surface is
Ordinate, the contact angle corresponding to the mass percent concentration of bio-adhesive agent aqueous solution carry out linear fit, are connect
The mass percent concentration c of bio-adhesive agent is 100 substitution contact angle fit equations, calculates biology by feeler fit equation
Contact angle θ of the binder on No.1 detected materials surface；
(3), test and calculate roll angle: the biology for taking bio-adhesive agent to be configured to 6-10 parts of 0.1-1mg/mL respectively with water again is glutinous
Attached dose of aqueous solution rises the concentration of bio-adhesive agent aqueous solution successively in gradient, using inclined-plane with a scale test respectively 6 to
10 parts of bio-adhesive agent aqueous solutions No.1 detected materials surface roll angle, every part of bio-adhesive agent aqueous solution test 5 it is flat
Row roll angle degree calculates average value, glutinous with biology by the concentration conversion of bio-adhesive agent aqueous solution at mass percent concentration
Attached dose of mass percent concentration be abscissa, using bio-adhesive agent No.1 detected materials surface roll angle as ordinate,
The roll angle corresponding to the mass percent concentration of bio-adhesive agent aqueous solution carries out linear fit, obtains roll angle fitting
The mass percent concentration c of bio-adhesive agent is 100 substitution roll angle fit equations, calculates bio-adhesive agent and exist by equation
The roll angle on No.1 detected materials surface
(4), it calculates anti-sticking coefficient: using the blank sample of glass slide or blank coating as control test material, use
Step (2) and step (3) test respectively and calculate bio-adhesive agent in the contact angle θ on control test material surfaceXAnd rolling
AngleBy bio-adhesive agent in No.1 detected materials and the contact angle θ and θ that compare test material surfaceXSubstitute into formula:Obtain contact angle coefficient of correlation f, by bio-adhesive agent No.1 detected materials with compare test material table
The roll angle in faceWithSubstitute into formula:Obtain roll angle coefficient of correlation fX, use f-fXNo.1 is calculated to wait for
Measure and monitor the growth of standing timber the antibiont binder sticking coefficient Aad of material；Or by bio-adhesive agent No.1 detected materials with compare test material
The contact angle θ and θ on surfaceXAnd bio-adhesive agent is in No.1 detected materials and the roll angle for compareing test material surfaceWith
Substitute into formula:The antibiont binder sticking coefficient of No.1 detected materials is calculated
Aad；
(5), it evaluates anti-pollution: experiment being compared to multiple detected materials simultaneously according to step (2), (3) and (4), is obtained
The antibiont binder sticking coefficient of each detected materials, Aad numerical value is smaller, and bio-adhesive agent is easier on detected materials surface
Absorption, shows that the antibiont binder Adhesion property of detected materials is weaker, Aad numerical value is bigger, and bio-adhesive agent is less susceptible to
Detected materials adsorption shows that the antibiont binder Adhesion property of detected materials is stronger.
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