CN106492110A - A kind of sobering-up composition, the relieving alcoholism and protecting the liver preparation comprising which and its application - Google Patents

Abstract

The present invention provides a kind of sobering-up composition, the relieving alcoholism and protecting the liver preparation comprising which and its application, and the sobering-up composition includes following component：Maca extract, Radix Puerariae extract, Rhizoma Curcumae Longae extract and Japanese raisintree fruit extract.Sobering-up composition of the present invention carries out scientific and reasonable compounding through selecting specified raw material, can promote ethanol quick absorption in vivo, decomposition, be conducive to ethanol to take the photograph quickly relieving the effect of alcohol for taker, prevent from being still drank after a night, with preferably liver-protective effect.Sobering-up composition of the present invention can be prepared into relieving alcoholism and protecting the liver preparation or be applied in hepatoprotective functional food.

Description

A hangover-relief composition, a hangover-protection preparation containing the same and application thereof

technical field

The invention belongs to the technical field of traditional Chinese medicine, and relates to a hangover-relief composition, a hangover-relief and liver-protecting preparation containing the same and an application thereof.

Background technique

The liver is the central hub of carbohydrate metabolism, lipid metabolism and protein metabolism in the human body, and has important functions such as detoxification and bile secretion. Alcohol is mainly absorbed through the stomach (20%) and duodenum (80%) after drinking, and about 90% of the alcohol is metabolized in the liver. Alcohol has cytotoxicity, peroxidizes the lipids on the surface of the liver cell membrane, destroys the liver cell membrane, and further develops the structure of the microtubules and mitochondria in the liver cells, which makes the metabolism in the cells disordered and leads to cytotoxic metabolites resulting in swelling and necrosis of liver cells. Nowadays, under the influence of wine table culture, many people drink excessively, which exceeds the limit of human alcohol metabolism, which causes liver damage and decline of liver function, which poses a great threat to human health.

Western medicine has a good effect in a short period of time, but it cannot treat and restore the human body systematically, and it also has great side effects. The essence of health functional food is food, which contains a certain amount of functional ingredients, which can regulate the function of the human body, maintain health or prevent certain diseases. It is safe and non-toxic, and benefits a wide range of people; Health products have broad market prospects.

CN101716001A discloses a hangover-protecting liver drink and its production method. The raw materials are composed according to the following weight ratio: 15% kudzu root, 10% capillary, 10% kudzu flower, 10% Luo Han Guo, 10% tamarind, and 10% husk , Chess pieces 10%, licorice 10%, tangerine peel 15%, pectin 5%, mint 3%, the raw materials are washed, crushed, soaked, heated, filtered, clarified, flavored, gummed and filtered, sterilized and filled Finished product; it has the effects of promoting gallbladder, protecting liver, diuresis, inhibiting gastrointestinal absorption, and detoxification.

CN101972462A discloses a hangover health care product that can relieve alcohol and eliminate dampness, replenish qi and invigorate the spleen, and promote qi and diuresis. 50 parts, 10-30 parts of chrysanthemum, 5-20 parts of coriander root, 5-20 parts of red bean, 5-10 parts of black plum, 5-10 parts of dried ginger, 5-20 parts of hawthorn, 5-20 parts of fried malt; Added: 5-10 parts of Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizoma 5-10 parts; this formula is through compatibility, and comprehensively uses methods such as promoting qi, promoting water, nourishing deficiency, harmonizing the stomach, and refreshing the spleen to regulate the functions of viscera and viscera, and the effect of hangover is rapid and the effect is obvious.

In this field, the development of traditional Chinese medicine anti-alcoholic drugs has a good application prospect, and it is expected to obtain more safe and effective traditional Chinese medicine compositions that have protective and auxiliary therapeutic effects on alcoholic liver damage.

Contents of the invention

Aiming at the deficiencies of the prior art, the object of the present invention is to provide a hangover-relief composition, a hangover-relief and liver-protecting preparation containing the same, and its application. The anti-alcoholic composition of the invention has protective function and auxiliary therapeutic effect on alcoholic liver injury, and has good pharmacological effects.

To achieve this purpose of the invention, the present invention adopts the following technical solutions:

In one aspect, the present invention provides a hangover hangover composition, which comprises the following raw material extract components: Maca extract, Pueraria root extract, Turmeric extract and Hovenia dulcis fruit extract.

The anti-alcoholic composition of the present invention is compatible with the herbal plants such as maca extract, kudzu root extract, turmeric extract and hovenia dulcis extract as basic raw materials, and has protective and therapeutic effects on alcoholic liver damage. Solve the real problem of increasing liver damage caused by drinking.

In the present invention, maca extract, kudzu root extract, turmeric extract and Hovenia dulcis fruit extract are scientifically and rationally compounded, and each component cooperates with each other within the dosage range, so that the effects of hangover and liver protection can be exerted , the lack of any one or two components, or the change in the dosage range of one or some components will affect its anti-alcohol and liver-protecting effect.

Preferably, the anti-alcohol composition comprises the following raw material extract components in parts by weight:

In the hangover composition of the present invention, the amount of the maca extract can be 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts, 18 parts, 20 parts, 22 or 25 servings.

In the anti-alcohol composition of the present invention, the amount of the kudzu root extract can be 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts, 18 parts or 20 parts.

In the hangover composition of the present invention, the dosage of the turmeric extract can be 0.3 parts, 0.6 parts, 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts, 18 parts servings, 20 servings, 22 servings or 25 servings.

In the hangover composition of the present invention, the dosage of the Hovenia dulcis fruit extract can be 0.5 parts, 0.8 parts, 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 14 parts, 16 parts , 18, 20, 22 or 25 copies.

Preferably, the anti-alcohol composition comprises the following raw material extract components in parts by weight:

Further preferably, the hangover hangover composition comprises the following raw material extract components in parts by weight:

In the present invention, the anti-alcoholic composition further includes any one of tangerine peel extract, cistanche extract, medlar extract, yam extract, hawthorn extract, jujube extract, licorice extract or Poria cocos extract. one or a combination of at least two. The combination can be the combination of tangerine peel extract and cistanche extract, the combination of tangerine peel extract and medlar extract, the combination of tangerine peel extract and yam extract, the combination of tangerine peel extract and hawthorn extract, jujube extract and licorice Combination of extracts, combination of licorice extract and Poria cocos extract, combination of cistanche extract and wolfberry extract and yam extract, tangerine peel extract and wolfberry extract, yam extract, hawthorn extract, jujube extract Combination of licorice extract, licorice extract and Poria cocos extract.

Preferably, the hangover hangover composition further comprises 1-7 parts by weight (for example, 1 part by weight, 1.5 parts by weight, 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight or 7 parts by weight) Tangerine Peel Extract.

Preferably, the anti-hangover composition comprises 1-8 parts by weight (for example, 1 part by weight, 2 parts by weight, 3 parts by weight, 4 parts by weight, 5 parts by weight, 6 parts by weight, 7 parts by weight or 8 parts by weight) of Herba Cistanche Extract.

Preferably, the anti-alcohol composition further comprises 1-6 parts by weight (for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts or 6 parts) of Lycium barbarum extract.

Preferably, the hangover-relief composition further comprises 1-5 parts by weight (for example, 1 part by weight, 2 parts by weight, 3 parts by weight, 4 parts by weight or 5 parts by weight) of Chinese yam extract.

Preferably, the hangover-relief composition further comprises 1-7 parts by weight (eg, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts or 7 parts) of hawthorn extract.

Preferably, the hangover composition further comprises 1-3 parts by weight (eg 1 part by weight, 1.3 parts by weight, 1.5 parts by weight, 1.8 parts by weight, 2 parts by weight, 2.5 parts by weight, 2.8 parts by weight or 3 parts by weight) Jujube Extract.

Preferably, the hangover composition further comprises 1-3 parts by weight (eg 1 part by weight, 1.3 parts by weight, 1.5 parts by weight, 1.8 parts by weight, 2 parts by weight, 2.5 parts by weight, 2.8 parts by weight or 3 parts by weight) Licorice extract.

Preferably, the hangover hangover composition further comprises 1-5 parts by weight (for example, 1 part, 2 parts, 3 parts, 4 parts or 5 parts by weight) of Poria cocos extract.

A typical but non-limiting example is: the hangover composition of the present invention comprises the following raw material extract components in parts by weight:

Another typical but non-limiting example is: the anti-alcoholic composition of the present invention comprises the following raw material extract components in parts by weight:

Another typical but non-limiting example is: the anti-alcoholic composition of the present invention comprises the following raw material extract components in parts by weight:

Another typical but non-limiting example is: the anti-alcoholic composition of the present invention comprises the following raw material extract components in parts by weight:

The hangover hangover composition of the present invention is prepared by mixing the above-mentioned raw material extract components.

The extract components of the raw materials mentioned above can be obtained by extracting the corresponding raw materials separately to obtain the above-mentioned extract components. It is also possible to mix the raw materials corresponding to the above-mentioned components for extraction, and the amount of the raw materials can be adjusted according to the formula ratio of the desired hangover composition to obtain the expected hangover composition.

Preferably, the mixed extraction process is as follows: mix and pulverize the raw materials, add water to soak for 1-2h (for example, 1h, 1.2h, 1.4h, 1.6h, 1.8h or 2h), and extract; Concentrate to a concentrated solution with a density of 1.02-1.06g/mL (such as 1.02g/mL, 1.03g/mL, 1.04g/mL, 1.05g/mL or 1.06g/mL) to obtain the hangover hangover composition;

Preferably, the process of mixed extraction is as follows: mixing and pulverizing the raw materials, soaking for 1-2h (such as 1h, 1.2h, 1.4h, 1.6h, 1.8h or 2h), and performing ultrasonic circulation extraction. Compression concentration to a density of 1.02-1.06g/mL (such as 1.02g/mL, 1.03g/mL, 1.04g/mL, 1.05g/mL or 1.06g/mL) to obtain a concentrated solution to obtain the hangover composition;

Preferably, the ultrasonic power during the ultrasonic cycle extraction is 300-2000W (such as 300W, 350W, 400W, 450W, 500W, 600W, 700W, 800W, 900W, 1000W, 1200W, 1500W, 1800W or 2000W), and the extraction temperature is 20-30°C (eg, 20°C, 22°C, 24°C, 26°C, 28°C or 30°C).

In another aspect, the present invention provides a hangover-relief and liver-protection preparation, which comprises the above-mentioned hangover-relief composition.

Preferably, the dosage form of the anti-alcoholic and liver-protecting preparation may be capsules, tablets, pills, oral liquids, ointments, gels or aerosols.

In the present invention, the anti-alcohol composition (ie the concentrated solution prepared above) can be directly prepared into oral liquid, ointment, gel or aerosol.

Preferably, the hangover-relief composition (ie the concentrated solution prepared above) is dried into powder and then prepared into capsules, tablets or pills. Preferably, the drying method is spray drying.

Preferably, the capsules are soft capsules. The shape of the soft capsule is ellipse, circle, sphere or drop shape, preferably ellipse. In the anti-alcoholic and liver-protecting preparation of the present invention, the soft capsule can overcome the shortcomings of the tablet. After the traditional tablet is swallowed by people, the drug ingredients go through processes such as disintegration, dissolution, and suction, which takes a long time and the drug effect is lost. Higher; at the same time, it is easy to cause moisture absorption and deterioration of ingredients. The soft capsule dosage form can be directly absorbed in the intestinal tract after disintegration without dissolving, and can effectively avoid the acid erosion of gastric acid and the digestion and absorption of the gastric wall, so it has precise content, high bioavailability, good pharmacological effect, and good stability of functional substances. It has good sealing performance, covers the bad smell of medicine, and is convenient to take.

In the present invention, the preparation method of the soft capsule comprises the following steps:

(1) Take each component according to the above formula, and mix to obtain a component mixture;

(2) Filling the component mixture into soft capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection and outer packaging to obtain the soft capsule.

In another aspect, the present invention provides the application of the hangover-relief composition in liver-protecting functional food. The anti-alcoholic composition of the present invention can be used in a liver-protecting and liver-protecting functional food in an appropriate dosage, so as to play a health care effect on the human body.

Compared with the prior art, the present invention has the following beneficial effects:

The anti-alcoholic composition of the present invention is scientifically and rationally compounded by selecting specific raw materials, and the results of animal experiments and human food trials show that the product can promote the rapid absorption and decomposition of alcohol in the body, which is beneficial to the rapid anti-alcoholic alcohol intake , to prevent hangovers, and has a better effect of protecting the liver.

Description of drawings

Fig. 1 is the change curve of each treatment group mouse body weight in embodiment 10;

FIG. 2 is a diagram showing the results of changes in liver organ coefficients of mice in each treatment group measured in Example 10. FIG.

Fig. 3 is the result figure of the serum ALT level of each treatment group mouse measured in embodiment 10;

Fig. 4 is the result figure of the serum AST level of each treatment group mouse measured in embodiment 10;

Fig. 5 is the result figure of the serum TG level of each treatment group mouse measured in embodiment 10;

Fig. 6 is the result figure of the serum LDL-c level of each treatment group mouse measured in embodiment 10;

Fig. 7 is the result figure of the serum CHO level of mice of each treatment group measured in embodiment 10;

Fig. 8 is the result figure of the serum gamma-GT level of mice of each treatment group measured in embodiment 10;

Fig. 9 is the result figure of the serum TBIL level of mice of each treatment group measured in embodiment 10;

Fig. 10 is the result figure of the serum ACP level of each treatment group mouse measured in embodiment 10;

Fig. 11 is the hepatic TG level result figure of each treatment group mouse measured in embodiment 10;

Fig. 12 is the hepatic SOD level result figure of each treatment group mouse measured in embodiment 10;

Fig. 13 is the hepatic MDA level result figure of each treatment group mouse measured in embodiment 10;

Fig. 14 is the liver GST level result figure of each treatment group mouse measured in embodiment 10;

Fig. 15 is the result figure of the liver GSHPX level of mice of each treatment group measured in embodiment 10;

Fig. 16 is the result figure of the liver GSH level of mice of each treatment group measured in embodiment 10;

Fig. 17 is the hepatic CAT level result figure of each treatment group mouse measured in embodiment 10;

FIG. 18 is a graph showing the results of liver LDH levels of mice in each treatment group measured in Example 10. FIG.

detailed description

The technical solutions of the present invention will be further described below through specific embodiments. It should be clear to those skilled in the art that the embodiments are only for helping to understand the present invention, and should not be regarded as specific limitations on the present invention.

Example 1

In this embodiment, the hangover composition consists of the following components in parts by weight:

Maca extract 1 part, kudzu root extract 3 parts, turmeric extract 5 parts and Hovenia dulcis extract 2 parts.

The preparation method is as follows: extracting maca, kudzu root, turmeric and hovenia dulcis respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula quantity to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 2

In this embodiment, the hangover composition consists of the following components in parts by weight:

Maca extract 5 parts, kudzu root extract 4 parts, turmeric extract 1 part and Hovenia dulcis seed extract 4 parts.

The preparation method is as follows: extracting maca, kudzu root, turmeric and hovenia dulcis respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula quantity to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 3

In this embodiment, the hangover composition consists of the following components in parts by weight:

10 parts of maca extract, 2 parts of kudzu root extract, 8 parts of turmeric extract and 6 parts of Hovenia dulcis seed extract.

The preparation method is as follows: extracting maca, kudzu root, turmeric and hovenia dulcis respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula quantity to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 4

In this embodiment, the hangover composition consists of the following components in parts by weight:

20 parts of maca extract, 8 parts of kudzu root extract, 10 parts of turmeric extract and 10 parts of Hovenia dulcis seed extract.

The preparation method is as follows: extracting maca, kudzu root, turmeric and hovenia dulcis respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula quantity to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 5

In this embodiment, the hangover composition consists of the following components in parts by weight:

25 parts of maca extract, 1 part of kudzu root extract, 5 parts of turmeric extract and 20 parts of Hovenia dulcis seed extract.

The preparation method is as follows: extracting maca, kudzu root, turmeric and hovenia dulcis respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula quantity to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 6

In this embodiment, the hangover composition consists of the following components in parts by weight:

Maca extract 3 parts, kudzu root extract 2 parts, turmeric extract 2 parts, Hovenia dulcis seed extract 1 part, Cistanche deserticola extract 1 part.

The preparation method is as follows: extracting maca, kudzu root, turmeric, Hovenia dulcis and Cistanche respectively to obtain the above-mentioned extracts, and then mixing the above components according to the formula amount to obtain the anti-alcohol composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 7

In this embodiment, the hangover composition consists of the following components by weight:

Maca extract 4 parts, kudzu root extract 3 parts, turmeric extract 1 part, Hovenia dulcis seed extract 2 parts, tangerine peel extract 2 parts and cistanche deserticola extract 2 parts.

The preparation method is as follows: the raw materials corresponding to the above-mentioned components are mixed for extraction, that is, the raw materials of each component in the anti-alcohol composition (ie maca, kudzu root, turmeric, Hovenia dulcis, tangerine peel and cistanche) are mixed and pulverized , add water and soak for 2 hours, and extract; after the extraction, concentrate under vacuum and reduce pressure to a concentrated solution with a density of 1.02g/mL. , inspection, and outer packaging steps to carry out capsule filling to obtain soft capsules.

Example 8

In this embodiment, the hangover composition consists of the following components in parts by weight:

Maca extract 3 parts, kudzu root extract 2 parts, turmeric extract 2 parts, Hovenia dulcis fruit extract 4 parts, licorice extract 4 parts, Poria cocos extract 1 part and wolfberry fruit extract 3 parts.

The preparation method is as follows: mixing and pulverizing the raw materials of each component in the anti-alcohol composition, soaking for 2 hours, and performing ultrasonic circulation extraction; the ultrasonic extraction conditions are ultrasonic power 1000W, temperature 25°C, and extraction for 1.5 hours; after the extraction, vacuum concentration The concentrated solution is obtained until the density is 1.04g/mL, and the concentrated solution is spray-dried to obtain a powder, which is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 9

In this embodiment, the hangover composition consists of the following components by weight:

Maca extract 1 part, kudzu root extract 4 parts, turmeric extract 1 part, Hovenia dulcis fruit extract 2 parts, licorice extract 3 parts, Poria cocos extract 3 parts, wolfberry fruit extract 2 parts, yam extract 1 part 1 part, hawthorn extract 1 part, jujube extract 1 part, tangerine peel extract 1 part.

The preparation method is as follows: each extract mentioned above is obtained by extracting maca, kudzu root, turmeric, Hovenia dulcis, licorice, poria cocos, medlar, yam, hawthorn, jujube and tangerine peel respectively, and then the above components are prepared according to The formula amounts are mixed to obtain a hangover-relief composition.

The above-mentioned anti-alcohol composition is filled into capsules through the steps of batching, pill pressing, shaping, drying, pill inspection, packaging, inspection, and outer packaging to obtain soft capsules.

Example 10

In this example, the hepatoprotective effect of the anti-alcohol composition prepared in Example 1 on mice with alcoholic liver injury was investigated. The specific experimental process is as follows:

Establish the alcoholic liver injury mouse model by intragastric administration of ethanol to ICR mice, and simultaneously intragastrically administer the anti-alcohol composition preparation (test sample E) prepared in Example 1, investigate the effect of the test sample on alcoholic liver injury mice Hepatoprotection in a model. After the end of the experiment, the mice were killed, and the biochemical and enzymatic indicators of hematology and liver tissue were measured, and the mechanism of action of the tested samples was preliminarily explored.

Experimental materials: A total of 60 ICR mice, female, weighing 22-25g, were raised in an SPF grade animal room. The animal room was illuminated for 12 hours with alternating light and dark, with a temperature of 22-26°C and a humidity of 40-60%. The mice were housed in IVC cages, 5 per cage, and the mice had free access to water and food during the experiment. After the mice were purchased, they were adaptively raised in the animal room for one week and then the formal experiment began. The operation and treatment of experimental animals complied with the requirements of the guidelines for ethical review of experimental animals.

Experimental method: Establishment of mouse model of alcoholic liver injury: 6-8 weeks old female ICR mice, after one week of adaptive feeding, were randomly divided into 6 groups according to body weight, 10 mice in each group, respectively: normal control group, model control group, positive drug bicyclol group, three high, middle and low dose groups of test sample E. From the first day of the experiment, mice were given 56-degree Erguotou wine orally at 2:00PM every day, and the ethanol dose in Erguotou wine was increased with time. The specific plan was ethanol 2g/kg×3 days, 4g/kg kg×4 days, 6g/kg×24 days. During the experiment, the mice in the normal control group were not given Erguotou wine, and the animals in this group were given the same volume of distilled water.

Pharmacodynamic evaluation based on the alcoholic liver injury mouse model: The pharmacodynamic evaluation of the test samples is carried out simultaneously with the establishment of the alcoholic liver injury mouse model. At 9 o'clock in the morning on the day when the mouse model of alcoholic liver injury was established (by gavage with Erguotou wine), the mice were gavaged with the test sample (the anti-alcoholic composition preparation prepared in Example 1). Among them, the normal control group and the model control group were given distilled water orally, and the positive control group was given bicyclol, with a dose of 300 mg/kg. and 1800mg/kg, all mice were gavaged with a volume of 10mL/kg.

The body weight of the mice was recorded twice a week during the experiment, and the body weight change data was summarized after the experiment. During the experiment, the general state of the mice was observed every day, and any abnormalities such as listlessness, bowed back, erect hair, obvious weight loss and death were recorded in time. Four hours after the last gavage of Erguotou wine (the 31st day of administration), blood was collected from the orbit of the mice, the serum was separated, and ALT, AST, ACP, GGT, TBIL, TG, CHO and LDL- c: The mice were sacrificed after blood collection, the abdominal cavity was opened to separate the liver, the total weight of the liver was measured, and the organ coefficient of the liver was calculated for statistical analysis. The organ coefficient of the liver = liver weight/body weight × 100%.

After the liver was weighed, a piece of liver tissue from the left lobe was fixed in formalin for tissue sectioning and HE staining, and then two small pieces of liver tissue were weighed separately, and one piece of liver tissue was homogenized to determine SOD, MDA, CAT, GSHPX, GST, LDH and GSH were weighed and then homogenized to determine the liver TG content (see Table 1 for animal experiment groups and dosing regimens).

Liver TG content determination method: Add 50 μL of 10% liver homogenate tissue, 50 μL of distilled water, 200 μL of methanol, and 400 μL of chloroform to a 1.5mL EP tube, mix well, take 200 μL of the organic phase liquid, add it to a new EP tube, and volatilize. Then fully dissolve with 500 μL isopropanol. Finally, a kit was used to determine the TG content.

Table 1

Statistical analysis: SPSS 22.0 was used for statistical analysis of data, One-way ANOVA was used for comparison of measurement data between groups, homogeneity of variance analysis and normal distribution test were performed before comparison, LSD test was used when variances were homogeneous, and Dunnett T3 was used when variances were not homogeneous test.

Experimental results: (1) Changes in general conditions, body weight and liver organ coefficients of animals

In the initial stage of the experiment, when the mice were given ethanol 2g/kg orally, none of the mice showed obvious drunkenness, and some mice became excited, and the running movement in the cage increased. When the amount of ethanol was increased to 4g/kg, some mice showed signs of drunkenness and central nervous system depression, such as unsteady gait, shortness of breath, scratching the cage wall, lying on their side and falling asleep until the righting reflex disappeared. When the amount of ethanol was added to 6g/kg, all the mice showed the above drunkenness, and the duration ranged from 30 minutes to 2 hours.

In this experiment, one mouse in the middle-dose group and the high-dose group of the test sample died on the 21st and 22nd day of the experiment, and no significant abnormality was found in the anatomy. No animals died in the other 4 groups of mice during the experiment. During the experiment, the weight of the mice in the normal control group increased gradually, and at the end of the experiment, the body weight increased by 28.9% compared with the initial body weight. Except for the normal control group, the body weight of the mice in the other groups increased slowly. After the ethanol gavage dose was increased to 6g/kg for one week, the mice in the other groups except the normal control group all lost weight. By the end of the experiment, the body weight of mice in each medication group increased by 2.2%-3.5% compared with the initial body weight (Fig. 1). In addition, the mice in the ethanol gavage group also had dull and untidy fur, and their spirits were worse than those in the normal control group.

After the experiment, the organ coefficient of the liver was measured, and the results showed that compared with the normal control group, the organ coefficient of the mouse liver in the model control group increased (4.4% vs 4.1%, P=0.109), and the liver organ coefficient of the mice in each group of test samples Compared with the model control group, the organ coefficient was lower (4.2% and 4.3%), but there was no statistical difference (Figure 2).

(2) Changes of hematological indicators in mice

After the experiment, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (CHO), γ-glutamyl transpeptidase (γ-GT), low-density lipid Protein cholesterol (LDL-c), acid phosphatase (ACP) and total bilirubin (TBIL), the results showed:

①Compared with the normal control group, the serum ALT and AST levels of the mice in the model control group were significantly increased (83.6U/L vs 53.5U/L and 235.3U/L vs 140.9U/L, respectively), indicating that the liver injury model was successful. The serum ALT of the mice in the medium and high dose groups of the test sample E was significantly lower than that of the model group (Figure 3), and the three doses of the sample E all reduced the serum AST of the mice (Figure 4).

②In the blood lipid index, compared with the normal control group, the serum TG and LDL-c levels of the model control group were significantly increased, and the three doses of sample E showed different degrees of TG reduction, but the dose-effect relationship was not significant (Figure 5) . In the LDL-c index, sample E significantly reduced the serum LDL-c level of mice, and showed a dose-dependent effect (Figure 6), and all samples had no obvious effect on the serum CHO level (Figure 7).

③In other indicators, compared with the normal control group mice, the serum γ-GT level of the model control group mice was significantly increased, and all the tested samples made the mouse serum γ-GT level (ie, the GGT level in Figure 8) decreased. decreased, but there was no statistical difference (Figure 8). In this experiment, there was no significant difference in serum TBIL and ACP levels of mice in each group (Figure 9 and Figure 10).

(3) Biochemical and enzymatic changes of mouse liver tissue

After the experiment, the mice were sacrificed, and a small piece of liver was accurately weighed to prepare a 10% homogenate, which was diluted to different concentrations according to the instructions of the kit, and TG, SOD, MDA, CAT, GSHPX, GST, LDH and GSH in the liver tissue were measured. The results show:

① Liver TG: Compared with the normal control group, the liver TG level of the mice in the model group was significantly increased (41.8mg/g liverweight vs 23.7mg/g liver weight), while all the tested samples decreased the liver TG level to varying degrees, Compared with the model control group, there was a significant statistical difference (Figure 11).

②Hepatic SOD and MDA: Compared with the normal control group, the liver SOD level of the model control group was significantly reduced (305.4U/mgprot vs 396.1U/mgprot). The medium and high doses of test sample E slightly increased the liver SOD level compared with the model group, but there was no statistical difference (Figure 12). Compared with the normal control group, the liver MDA level in the model control group was significantly increased (2.7nmol/mgprot vs 1.7nmol/mgprot). Sample E in the high-dose group reduced the level of MDA in the liver, which was statistically different from that in the model group (Fig. 13).

③ Liver GST, GSHPX, GSH and CAT: Compared with the normal control group, the liver GST and GSHPX levels of the mice in the model control group were significantly reduced. The three dose groups of sample E increased the level of GST in mice after administration, and the low dose group increased significantly ( FIG. 14 ). The level of GSHPX in the mice in the three dose groups of sample E was slightly increased after administration, but there was no statistical difference compared with the model group ( FIG. 15 ). Although the liver GSH and CAT levels of mice in the model control group were significantly lower than those in the normal control group, only the positive drug bicyclol showed an effect of increasing GSH and CAT in this experiment. Compared with the model control group, there was no statistical difference (Figure 16 and Figure 17).

④ Liver LDH: There was no significant difference in the level of liver LDH among the experimental groups (including model control and normal control group) (Figure 18).

The above experimental results prove that the anti-alcoholic composition preparation prepared in Example 1 has a protective effect on alcoholic liver injury and has an auxiliary therapeutic effect. The pharmacological effect of the anti-alcoholic composition is good, non-toxic, safe and effective.

The anti-alcoholic composition preparation prepared in Example 2-9 was subjected to the same experiment as in Example 10, and it was found that the anti-alcoholic composition preparation prepared in Example 2-9 also had a significant protective effect on the liver of alcoholic liver injury mice And auxiliary therapeutic effect, good pharmacological effect, safe and non-toxic.

Example 11

In this experiment, the soft capsules of the anti-alcoholic composition prepared in Examples 1-9 were carried out to a human body food trial test, and were given to 80 routine drinkers respectively, keeping other conditions of each group of experiments the same, observing and recording the effect of anti-alcoholism. The proportion of the effect, the results are shown in Table 2.

Table 2

As can be seen from Table 2, through the human food test, the results show that the anti-alcoholic composition preparation of the present invention can promote the rapid absorption and decomposition of alcohol in the body, which is conducive to the rapid anti-alcoholism of alcohol intake and prevent hangovers. It can also be added to food in an appropriate amount to obtain functional food, so as to protect the liver.

The applicant declares that the present invention illustrates the anti-alcoholic composition of the present invention and the anti-alcoholic liver-protecting preparation containing it and its application through the above-mentioned examples, but the present invention is not limited to the above-mentioned examples, that is, it does not mean that the present invention must Rely on the above-mentioned embodiment to implement. Those skilled in the art should understand that any improvement of the present invention, the equivalent replacement of the selected raw materials in the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.

Claims (10)

1. a kind of sobering-up composition, it is characterised in that the sobering-up composition includes the following raw material extract components：Lepidinm meyenii Walp is extracted Thing, Radix Puerariae extract, Rhizoma Curcumae Longae extract and Japanese raisintree fruit extract.

2. sobering-up composition according to claim 1, it is characterised in that the sobering-up composition is comprising following weight portion Raw extract component：

3. sobering-up composition according to claim 1 and 2, it is characterised in that the sobering-up composition includes following weight The raw extract component of part：

Preferably, raw extract component of the sobering-up composition comprising following weight portion：

4. the sobering-up composition according to any one of claim 1-3, it is characterised in that the sobering-up composition also includes Pericarpium Citri Reticulatae extract, Herba Cistanches extract, Fructus Lycii extract, Rhizoma Dioscoreae extract, Fructus Crataegi extract, Fructus Jujubae extract, Radix Glycyrrhizae carry Take in thing or Poria extract any one or at least two combination.

5. the sobering-up composition according to any one of claim 1-4, it is characterised in that the sobering-up composition also includes 1-7 weight portion Pericarpium Citri Reticulatae extract；

Preferably, the sobering-up composition also includes 1-8 weight portion Herba Cistanches extracts；

Preferably, the sobering-up composition also includes 1-6 weight portion Fructus Lycii extracts；

Preferably, the sobering-up composition also includes 1-5 weight portion Rhizoma Dioscoreae extracts；

Preferably, the sobering-up composition also includes 1-7 weight portion Fructus Crataegi extracts；

Preferably, the sobering-up composition also includes 1-3 weight portion Fructus Jujubae extracts；

Preferably, the sobering-up composition also includes 1-3 weight portion Radix Glycyrrhizae extracts；

Preferably, the sobering-up composition also includes 1-5 weight portion Poria extracts.

6. the sobering-up composition according to any one of claim 1-5, it is characterised in that the raw extract component is led to Cross and carry out mixed extraction and obtain after individually being extracted to raw material or mix raw material；

Preferably, the process of the mixed extraction is：Raw material mixed powder is broken, 1-2h is soaked, is extracted；Extraction terminates Afterwards, vacuum-concentrcted is the concentrated solution of 1.02-1.06g/mL to density, obtains the sobering-up composition；

Preferably, the process of the mixed extraction is：Raw material mixed powder is broken, 1-2h is soaked, ultrasonic and circulated extraction is carried out, is extracted After end, vacuum-concentrcted obtains concentrated solution to density for 1.02-1.06g/mL, obtains the sobering-up composition；

Preferably, the ultrasonic power during ultrasonic and circulated extraction is 300-2000W, and Extracting temperature is 20-30 DEG C.

7. a kind of relieving alcoholism and protecting the liver preparation, it is characterised in that the relieving alcoholism and protecting the liver preparation includes such as any one of claim 1-6 institute The sobering-up composition that states；

Preferably, the dosage form of the relieving alcoholism and protecting the liver preparation is capsule, tablet, pill, oral liquid, ointment, gel or gas Mist agent.

8. relieving alcoholism and protecting the liver preparation according to claim 7, it is characterised in that the sobering-up composition is directly prepared into mouth Take liquid, ointment, gel or aerosol；

Preferably, the sobering-up composition drying is prepared into capsule, tablet or pill into after powder；

Preferably, the mode of the drying is spray drying.

9. the relieving alcoholism and protecting the liver preparation according to claim 7 or 8, it is characterised in that the capsule is soft capsule；

Preferably, the soft capsule is prepared by the following method and obtains：

(1) each component is weighed according to as above formula, be mixed to get component mixture；

(2) component mixture is carried out through dispensing, pelleting, sizing, drying, inspection ball, packaging, inspection, outer package step Soft capsule fill, obtains the soft capsule.

10. application of the sobering-up composition according to any one of claim 1-6 in hepatoprotective functional food.